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hepatitis e: an emerging global disease - from discovery towards control and cure.hepatitis e is a systemic disease affecting the liver predominantly and caused by infection with the hepatitis e virus (hev). hev has marked genetic heterogeneity and is known to infect several animal species including pigs, boar, deer, mongoose, rabbit, camel, chicken, rats, ferret, bats and cutthroat trout. hev is the sole member of the family hepeviridae and has been divided into 2 genera: orthohepevirus (mammalian and avian hev) and piscihepevirus (trout hev). human hevs included within the ...201626344932
chronically infected wild boar can transmit genotype 3 hepatitis e virus to domestic pigs.hepatitis e virus (hev) causes acute hepatitis e in humans in developing countries, but sporadic and autochthonous cases do also occur in industrialized nations. in europe, food-borne zoonotic transmission of genotype 3 (gt3) has been associated with the consumption of raw and undercooked products from domestic pig and wild boar. as shown recently, naturally acquired hev gt3 replicates efficiently in experimentally infected wild boar and is transmissible from a wild boar to domestic pigs. genera ...201526344041
identification and genetic characterization of two porcine astroviruses from domestic piglets in china.porcine astrovirus are divided into five genotypes. in this study, we identified two porcine astroviruses (astv-ll-1 and astv-ll-2) by using sequence-independent single-primer amplification (sispa) on faecal specimens of healthy domestic piglets younger than 15 days. the detection rate for both was 2.82% (14/497). astv-lls were then sequenced and characterised. phylogenetic analysis revealed that they have the characteristics of porcine astrovirus (pastv) 2 and 5 and have some unique genetic fea ...201526338091
on-pump nonrobotic total endoscopic coronary artery bypass grafting: an animal study.total endoscopic coronary artery bypass grafting (tecab) is currently performed exclusively with the help of a telemanipulator. after extensive practice in a biomechanical model, the operation was performed as a nonrobotic approach in an animal study.201626331233
urinary hepcidin levels in iron-deficient and iron-supplemented piglets correlate with hepcidin hepatic mrna and serum levels and with body iron status.among livestock, domestic pig (sus scrofa) is a species, in which iron metabolism has been most intensively examined during last decade. the obvious reason for studying the regulation of iron homeostasis especially in young pigs is neonatal iron deficiency anemia commonly occurring in these animals. moreover, supplementation of essentially all commercially reared piglets with iron entails a need for monitoring the efficacy of this routine practice followed in the swine industry for several decad ...201526323096
evidence of long-term gene flow and selection during domestication from analyses of eurasian wild and domestic pig genomes.traditionally, the process of domestication is assumed to be initiated by humans, involve few individuals and rely on reproductive isolation between wild and domestic forms. we analyzed pig domestication using over 100 genome sequences and tested whether pig domestication followed a traditional linear model or a more complex, reticulate model. we found that the assumptions of traditional models, such as reproductive isolation and strong domestication bottlenecks, are incompatible with the geneti ...201526323058
soil fungal community shift evaluation as a potential cadaver decomposition indicator.fungi metabolise organic matter in situ and so alter both the bio-/physico-chemical properties and microbial community structure of the ecosystem. in particular, they are responsible reportedly for specific stages of decomposition. therefore, this study aimed to extend previous bacteria-based forensic ecogenomics research by investigating soil fungal community and cadaver decomposition interactions in microcosms with garden soil (20 kg, fresh weight) and domestic pig (sus scrofa domesticus) carc ...201526322496
domestication and cereal feeding developed domestic pig-type intestinal microbiota in animals of suidae.intestinal microbiota are characterized by host-specific microorganisms, which have been selected through host-microbe interactions under phylogenetic evolution and transition of feeding behavior by the host. although many studies have focused on disease-related intestinal microbiota, the origin and evolution of host-specific intestinal microbiota have not been well elucidated. pig is the ideal mammal model to reveal the origin and evolution of host-specific intestinal microbiota because their d ...201626315488
high prevalence of tritrichomonas foetus 'bovine genotype' in faecal samples from domestic pigs at a farm where bovine trichomonosis has not been reported for over 30 years.bovine venereal trichomonosis caused by the flagellate tritrichomonas foetus is a notifiable disease in australia. while, t. foetus is pathogenic in both cattle and cats, it has long been established that the same t. foetus colonises the stomach, caecum and nasal cavity of pigs without apparent clinical significance. multi-locus genotyping grouped the non-pathogenic porcine t. foetus with the pathogenic 'bovine genotype', rather than with the 'feline genotype' t. foetus. bovine trichomonosis is ...201526315128
spontaneous dilated cardiomyopathy and right-sided heart failure as a differential diagnosis for hepatosis dietetica in a production pig.an experimentally naïve 37.7-kg yorkshire-crossbred gilt died unexpectedly 2 d after arrival. necropsy revealed severe dilated cardiomyopathy characterized grossly by markedly dilated ventricles and thinned ventricular walls and interventricular septum. histologically there was multifocal myofiber attenuation and patchy loss of myofiber cross striations. the liver contained submassive to massive, diffuse hepatic centrilobular hemorrhage and degeneration. these lesions supported a diagnosis of di ...026310462
assessing the potential role of pigs in the epidemiology of ebola virus in uganda.uganda has experienced 4 ebola outbreaks since the discovery of the virus. recent epidemiological work has shown pigs are hosts for ebola viruses. due to their high reproduction rates, rapid weight gain, potential to provide quick financial returns and rising demand for pork, pig production in uganda has undergone massive expansion. the combination of pork sector growth supported by development programmes and ebola virus risk prompted a foresight exercise using desk, interview and spatial method ...201726310206
one health, multiple challenges: the inter-species transmission of influenza a virus.influenza a viruses are amongst the most challenging viruses that threaten both human and animal health. influenza a viruses are unique in many ways. firstly, they are unique in the diversity of host species that they infect. this includes waterfowl (the original reservoir), terrestrial and aquatic poultry, swine, humans, horses, dog, cats, whales, seals and several other mammalian species. secondly, they are unique in their capacity to evolve and adapt, following crossing the species barrier, i ...026309905
porcine survival model to simulate acute upper gastrointestinal bleedings.the existing animal models used for the simulation of acute gastrointestinal bleedings are usually non-survival models. we developed and evaluated a new porcine model (domestic pig, german landrace) in which the animal remains alive and survives the artificial bleeding without any cardiovascular impairment. this consists of a bleeding catheter which is implanted into the stomach, then subcutaneously tunnelled from the abdomen to the neck where it is exteriorized and fixed with sutures. using the ...201626306615
first molecular assessment of the african swine fever virus status of ornithodoros ticks from swaziland.african swine fever (asf) is an economically significant haemorrhagic disease of domestic pigs. it is caused by the african swine fever virus (asfv), a deoxyribonucleic acid (dna)arbovirus. argasid ticks of the genus ornithodoros, which are widely distributed throughout southern africa, play a primary role in virus maintenance and spread within the endemic sylvatic cycle. the asf status of swaziland is unknown, but this land-locked country is surrounded by asf-positive countries, has a burgeonin ...201426304186
dynamics of virus shedding and antibody responses in influenza a virus-infected feral swine.given their free-ranging habits, feral swine could serve as reservoirs or spatially dynamic 'mixing vessels' for influenza a virus (iav). to better understand virus shedding patterns and antibody response dynamics in the context of iav surveillance amongst feral swine, we used iav of feral swine origin to perform infection experiments. the virus was highly infectious and transmissible in feral swine, and virus shedding patterns and antibody response dynamics were similar to those in domestic swi ...201526297148
distribution of porcine endogenous retrovirus in different organs of the hybrid of a landrace and a jeju domestic pig in korea.xenotransplantation offers a solution to the shortage of available organs for transplantation, and the pig represents an ideal source of such organs. however, porcine endogenous retrovirus (perv), whose genome is integrated in pigs, has been suggested to pose a potential risk of xenotransmission. expression of pervs in different organs of pigs was carefully measured at dna, mrna, and protein levels, providing information valuable for the application of pig organs in xenotransplantation. an analy ...201526293098
exploring evidence of positive selection reveals genetic basis of meat quality traits in berkshire pigs through whole genome sequencing.natural and artificial selection following domestication has led to the existence of more than a hundred pig breeds, as well as incredible variation in phenotypic traits. berkshire pigs are regarded as having superior meat quality compared to other breeds. as the meat production industry seeks selective breeding approaches to improve profitable traits such as meat quality, information about genetic determinants of these traits is in high demand. however, most of the studies have been performed u ...201526289667
hypoxanthine-guanine phosphoribosyltransferase and inosine 5'-monophosphate dehydrogenase activities in three mammalian species: aquatic (mirounga angustirostris), semi-aquatic (lontra longicaudis annectens) and terrestrial (sus scrofa).aquatic and semiaquatic mammals have the capacity of breath hold (apnea) diving. northern elephant seals (mirounga angustirostris) have the ability to perform deep and long duration dives; during a routine dive, adults can hold their breath for 25 min. neotropical river otters (lontra longicaudis annectens) can hold their breath for about 30 s. such periods of apnea may result in reduced oxygen concentration (hypoxia) and reduced blood supply (ischemia) to tissues. production of adenosine 5'-tri ...201526283971
clonal transmission of a colistin-resistant escherichia coli from a domesticated pig to a human in laos. 201526283671
effects of dietary protein/energy ratio on growth performance, carcass trait, meat quality, and plasma metabolites in pigs of different genotypes.the protein/energy ratio is important for the production performance and utilization of available feed resources by animals. increased protein consumption by mammals leads to elevated feed costs and increased nitrogen release into the environment. this study aimed to evaluate the effects of dietary protein/energy ratio on the growth performance, carcass traits, meat quality, and plasma metabolites of pigs of different genotypes.201526279834
increasing venoarterial extracorporeal membrane oxygenation flow negatively affects left ventricular performance in a porcine model of cardiogenic shock.the aim of this study was to assess the relationship between extracorporeal blood flow (ebf) and left ventricular (lv) performance during venoarterial extracorporeal membrane oxygenation (va ecmo) therapy.201526275717
a serosurvey for brucellosis in wild boar (sus scrofa) in sardinia, italy.porcine brucellosis is a zoonotic disease caused by brucella suis and hosted by pigs (sus scrofa). both domestic pigs and wild boars are affected. we measured the prevalence of antibody to brucella spp. in wild boars in sardinia, italy. during 1 november 2009 to 31 january 2010, we collected 570 serum samples from legally hunted wild boars and tested them using a commercial competitive enzyme-linked immunosorbent assay. sex and age class of the sampled wild boars were also recorded. thirty-five ...201526267463
a large-scale study of the trichinella genus in the golden jackal (canis aureus) population in serbia.over the last decades the golden jackal (canis aureus) has significantly expanded its range throughout southeast and central europe, and the balkan peninsula is considered to be a core area of the species distribution in this part of the range. due to its increasing number, ability of long distance movement through a wide range of landscapes and opportunistic feeding habits, the golden jackal may represent an important reservoir and transmitter of a variety of zoonotic agents, including parasite ...201526260409
disease risks associated with free-ranging wild boar in saskatchewan.this study investigated the disease status of saskatchewan's feral wild boar population. whole carcasses, tissue samples, and/or serum from 81 hunter-killed boars from saskatchewan were submitted to the canadian wildlife health cooperative (cwhc) between 2009 and 2014. serological tests were negative for prrs, h1n1, and h3n2 swine influenza, pcv-2, and tge/prcv in 22/22 boars and for toxoplasma gondii and mycoplasma hyopneumoniae in 20/20 boars. of 20 boars whose sera were tested 20 were positiv ...026246630
genomic diversity and differentiation of a managed island wild boar population.the evolution of island populations in natural systems is driven by local adaptation and genetic drift. however, evolutionary pathways may be altered by humans in several ways. the wild boar (wb) (sus scrofa) is an iconic game species occurring in several islands, where it has been strongly managed since prehistoric times. we examined genomic diversity at 49 803 single-nucleotide polymorphisms in 99 sardinian wbs and compared them with 196 wild specimens from mainland europe and 105 domestic pig ...201526243137
a triple culture model of the blood-brain barrier using porcine brain endothelial cells, astrocytes and pericytes.in vitro blood-brain barrier (bbb) models based on primary brain endothelial cells (becs) cultured as monoculture or in co-culture with primary astrocytes and pericytes are useful for studying many properties of the bbb. the becs retain their expression of tight junction proteins and efflux transporters leading to high trans-endothelial electric resistance (teer) and low passive paracellular permeability. the becs, astrocytes and pericytes are often isolated from small rodents. larger species as ...201526241648
a pharmacologic activator of endothelial kca channels increases systemic conductance and reduces arterial pressure in an anesthetized pig model.ska-31, an activator of endothelial kca2.3 and kca3.1 channels, reduces systemic blood pressure in mice and dogs, however, its effects in larger mammals are not well known. we therefore examined the hemodynamic effects of ska-31, along with sodium nitroprusside (snp), in anesthetized, juvenile male domestic pigs. experimentally, continuous measurements of left ventricular (lv), aortic and inferior vena cava (ivc) pressures, along with flows in the ascending aorta, carotid artery, left anterior d ...201626239885
new insights into the molecular epidemiology of trichinella infection in domestic pigs, wild boars, and bears in romania.trichinellosis is a food-borne zoonosis caused by the parasitic nematode trichinella, characterized by an extremely wide host range and geographical distribution. in romania, it is recognized as one of the most serious zoonotic diseases. a cross-sectional study, covering all regions of romania, was conducted in 2014 to investigate and update the prevalence of trichinella infection among domestic pigs, wild boars, and bears. additional, molecular identification of trichinella species circulating ...201526238657
characterization of a porcine model for associating liver partition and portal vein ligation for a staged hepatectomy.publications using the alpps (associating liver partition and portal vein ligation for a staged hepatectomy) procedure have demonstrated a future liver remnant growth of 40-160% in only 6-9 days. the present study aimed to develop and describe the first large animal model of alpps that can be used for future studies.201526234167
segmental and age differences in the elastin network, collagen, and smooth muscle phenotype in the tunica media of the porcine aorta.the porcine aorta is often used in studies on morphology, pathology, transplantation surgery, vascular and endovascular surgery, and biomechanics of the large arteries. using quantitative histology and stereology, we estimated the area fraction of elastin, collagen, alpha-smooth muscle actin, vimentin, and desmin within the tunica media in 123 tissue samples collected from five segments (thoracic ascending aorta; aortic arch; thoracic descending aorta; suprarenal abdominal aorta; and infrarenal ...201526232584
mechanistic insights into glucan phosphatase activity against polyglucan substrates.glucan phosphatases are central to the regulation of starch and glycogen metabolism. plants contain two known glucan phosphatases, starch excess4 (sex4) and like sex four2 (lsf2), which dephosphorylate starch. starch is water-insoluble and reversible phosphorylation solubilizes its outer surface allowing processive degradation. vertebrates contain a single known glucan phosphatase, laforin, that dephosphorylates glycogen. in the absence of laforin, water-soluble glycogen becomes insoluble, leadi ...201526231210
quantitative risk assessment of human trichinellosis caused by consumption of pork meat sausages in argentina.in argentina, there are three known species of genus trichinella; however, trichinella spiralis is most commonly associated with domestic pigs and it is recognized as the main cause of human trichinellosis by the consumption of products made with raw or insufficiently cooked pork meat. in some areas of argentina, this disease is endemic and it is thus necessary to develop a more effective programme of prevention and control. here, we developed a quantitative risk assessment of human trichinellos ...201626227185
the evolution of stomach acidity and its relevance to the human microbiome.gastric acidity is likely a key factor shaping the diversity and composition of microbial communities found in the vertebrate gut. we conducted a systematic review to test the hypothesis that a key role of the vertebrate stomach is to maintain the gut microbial community by filtering out novel microbial taxa before they pass into the intestines. we propose that species feeding either on carrion or on organisms that are close phylogenetic relatives should require the most restrictive filter (meas ...201526222383
influence of internal fixation systems on radiation therapy for spinal tumor.in this study, the influence of internal fixation systems on radiation therapy for spinal tumor was investigated in order to derive a theoretical basis for adjustment of radiation dose for patients with spinal tumor and internal fixation. based on a common method of internal fixation after resection of spinal tumor, different models of spinal internal fixation were constructed using the lumbar vertebra of fresh domestic pigs and titanium alloy as the internal fixation system. variations in radia ...201526219011
lymphatic territories (lymphosomes) in swine: an animal model for future lymphatic research.the swine is a common preclinical large-animal model for medical research because of the resemblance of its tissue structures to those of humans. however, the lymphatic system in swine is poorly understood. the authors investigated the lymphatic system and defined territories (lymphosomes) in swine using the microinjection technique.201526218378
using bacterial and necrophagous insect dynamics for post-mortem interval estimation during cold season: novel case study in romania.considering the biogeographical characteristics of forensic entomology, and the recent development of forensic microbiology as a complementary approach for post-mortem interval estimation, the current study focused on characterizing the succession of necrophagous insect species and bacterial communities inhabiting the rectum and mouth cavities of swine (sus scrofa domesticus) carcasses during a cold season outdoor experiment in an urban natural environment of bucharest, romania. we monitored the ...201526217916
a novel porcine bocavirus harbors a variant np gene.porcine bocavirus is classified within the genus bocaparvovirus, family parvoviridae. unlike other parvoviruses, the members of genus bocaparvovirus (bocaparvoviruses) encode an additional open reading frame (np1). many strains of pbovs have been identified in domestic pigs and recognized as a potential emerging pathogen causing respiratory and gastrointestinal disease.201526217547
effect of body position on ventilation distribution during peep titration in a porcine model of acute lung injury using advanced respiratory monitoring and electrical impedance tomography.lung failure after acute lung injury remains a challenge in different clinical settings. various interventions for restoration of gas exchange have been investigated. recruitment of collapsed alveoli by positive end expiratory pressure (peep) titration and optimization of ventilation-perfusion ratio by prone positioning have been extensively described in animal and clinical trials. this animal study was conducted to investigate the effects of peep and positioning by means of advanced respiratory ...201526215805
epitope recognition in the human-pig comparison model on fixed and embedded material.the conditions and the specificity by which an antibody binds to its target protein in routinely fixed and embedded tissues are unknown. direct methods, such as staining in a knock-out animal or in vitro peptide scanning of the epitope, are costly and impractical. we aimed to elucidate antibody specificity and binding conditions using tissue staining and public genomic and immunological databases by comparing human and pig-the farmed mammal evolutionarily closest to humans besides apes. we used ...201526209082
1ppab5. acoustic radiation force to reposition kidney stones.our group has introduced transcutaneous ultrasound to move kidney stones in order to expel small stones or relocate an obstructing stone to a nonobstructing location. human stones and metalized beads (2-8 mm) were implanted ureteroscopically in kidneys of eight domestic swine. ultrasonic propulsion was performed using a diagnostic imaging transducer and a verasonics ultrasound platform. stone propulsion was visualized using fluoroscopy, ultrasound, and the ureteroscope. successful stone movement ...201726207161
safety and efficacy of new sirolimus-eluting stent models in a preclinical study.initial preclinical studies are required during the process of improving polymers, platforms, and drug-eluting systems for new coronary stent designs. our objective was to analyze the efficacy and safety of new drug-eluting stent models compared with a conventional stent and commercialized drug-eluting stents in an experimental model with healthy porcine coronary arteries.201526206247
the first report of trichinella pseudospiralis presence in domestic swine and t. britovi in wild boar in bosnia and herzegovina.the balkans is endemic for nematodes of the genus trichinella in both domestic and wild animals. the high prevalence of these zoonotic pathogens in animals linked with the food habits to consume raw meat and meat derived products resulted in a very high prevalence of trichinellosis in humans living in this european region. in spite of numerous epidemiological investigations carried out in this region, very few information is available on the trichinella species circulating in bosnia and herzegov ...201526204186
topical antibiotic ointment versus silver-containing foam dressing for second-degree burns in swine.second-degree burns are very common but their management is controversial. these burns may be treated with either topical antimicrobial agents or advanced occlusive dressings; however, there is no established treatment comparator for preclinical studies. this study was designed to determine which of two commonly used comparator therapies (a silver-containing advanced dressing or a topical antibiotic ointment) resulted in faster reepithelialization and less scarring. the hypothesis was that secon ...201526202791
serologic and molecular survey for hepatitis e virus in wild boar (sus scrofa) in central italy.the aim of this study was to further investigate the role of wild boar (sus scrofa) as a reservoir for hepatitis e virus (hev). sixty-four blood and faecal samples collected from wild boar hunted in central italy in 2011-2012 were examined by indirect enzyme-linked immunosorbent assay and rt-pcr analysis. positive rt-pcr samples were further examined by nucleotide sequence determination and subsequent phylogenetic analysis. thirty-six sera (56.2%) were positive for hev-specific antibodies, and s ...201526199731
statistical exploration of local transmission routes for african swine fever in pigs in the russian federation, 2007-2014.african swine fever (asf) is a devastating viral disease of swine that is present in both pigs and wild boar in the western part of the russian federation and the eastern part of the european union. it represents a significant threat for the european pig production industry as neither treatment nor vaccine is available. this study analysed the spatial and spatio-temporal distributions of asf cases that were reported in domestic pigs and wild boar for assessing the likelihood of wild boar-to-dome ...201726192820
experimental evaluation of faecal escherichia coli and hepatitis e virus as biological indicators of contacts between domestic pigs and eurasian wild boar.domestic pigs and eurasian wild boar (sus scrofa) share several important viral and bacterial pathogens. therefore, direct and indirect contacts between domestic pigs and wild boar present a risk of pathogen spillover and can lead to long-term perpetuation of infection. biological indicators could be a powerful tool to understand and characterize contacts between wild boar and domestic pigs. here, faecal escherichia coli and hepatitis e virus (hev) were explored as potential biological indicator ...201726190581
the red fox (vulpes vulpes) plays a minor role in the epidemiology of the domestic cycle of trichinella in romania.nematode worms of the genus trichinella are zoonotic parasites with a worldwide distribution. the majority of the biomass of these nematodes circulates among wildlife, but when humans fail in the proper management of domestic animals and wildlife, trichinella infections are transmitted from the sylvatic to the domestic environment. such failures occur in romania, where a high prevalence of trichinella spiralis has been detected in domestic pigs. the aim of the present study was to provide data a ...201526185060
molecular evidence of ebola reston virus infection in philippine bats.in 2008-09, evidence of reston ebolavirus (restv) infection was found in domestic pigs and pig workers in the philippines. with species of bats having been shown to be the cryptic reservoir of filoviruses elsewhere, the philippine government, in conjunction with the food and agriculture organization of the united nations, assembled a multi-disciplinary and multi-institutional team to investigate philippine bats as the possible reservoir of restv.201526184657
introducer development for coronary sinus access from parasternal mediastinotomy.right parasternal mediastinotomy with right atriotomy has been used clinically for pacemaker insertion. a similar approach might facilitate access to the coronary sinus for biventricular pacing and other manipulations when more conventional approaches are not feasible. the primary barrier to this is lack of appropriate introducers and techniques.201526181586
evaluation of an erns-based enzyme-linked immunosorbent assay to distinguish classical swine fever virus-infected pigs from pigs vaccinated with cp7_e2alf.infections with classical swine fever virus (csfv) are a major economic threat to pig production. to combat csf outbreaks and to maintain trade, new marker vaccines were developed that allow differentiation of infected from vaccinated animals (diva principle). the chimeric pestivirus cp7_e2alf was shown to be safe and efficacious. its diva strategy is based on the detection of csfv e(rns)-specific antibodies that are only developed on infection. however, for the new marker vaccine to be consider ...201526179095
pbmc transcriptomic responses to primary and secondary vaccination differ due to divergent lean growth and antibody titers in a pig model.the genetic relationship between immune responsiveness and performance is not well understood, but a major topic of the evolution of resource allocation and of relevance in human medicine and livestock breeding, for instance. this study aims to show differences of transcript abundance changes during the time intervals before and after two tetanus toxoid (tt) vaccinations in domestic pigs differing in lean growth (lg) and anti-tt-antibody titers (ab) parameters of performance and immunocompetence ...201526175500
increased b and t cell responses in m. bovis bacille calmette-guérin vaccinated pigs co-immunized with plasmid dna encoding a prototype tuberculosis antigen.the only tuberculosis vaccine currently available, bacille calmette-guérin (bcg) is a poor inducer of cd8(+) t cells, which are particularly important for the control of latent tuberculosis and protection against reactivation. as the induction of strong cd8(+) t cell responses is a hallmark of dna vaccines, a combination of bcg with plasmid dna encoding a prototype tb antigen (ag85a) was tested. as an alternative animal model, pigs were primed with bcg mixed with empty vector or codon-optimized ...201526172261
experimental study of temperature-sensitive chitosan/β-glycerophosphate embolic material in embolizing the basicranial rete mirabile in swines.the aim of the present study was to evaluate the feasibility of the non-adhesive temperature-sensitive liquid embolic material, chitosan/β-glycerophosphate (c/gp), in embolizing the basicranial rete mirabile (rem) in a swine model of cerebral arteriovenous malformation (cavm). a total of 24 domestic swines were used as the experimental animals, among which 12 pigs underwent direct embolization of one side of the rem, while the other 12 pigs underwent embolization of the bilateral rem following a ...201526170955
radionuclide therapy of unresectable tumors with avidinox and (90)y-biotindota: tongue cancer paradigm.local treatment of unresectable tumors is challenging, particularly with radioactivity. current practice relies on external beam irradiation or on a variety of medical devices for brachytherapy. both approaches proved useful in controlling tumor growth, but are characterized by poor compliance of the patient, significant side-effects, high costs, and technological complexity, which hamper widespread use. the authors recently described a novel form of radionuclide therapy based on the oxidized fo ...201526167947
new porcine epidemic diarrhoea virus variant with a large deletion in the spike gene identified in domestic pigs.from october 2013 to date, approximately 1,000 outbreaks of porcine epidemic diarrhoea virus (pedv) have occurred in japan. porcine epidemic diarrhoea with non-lethal effects in piglets was identified in tottori prefecture in october 2014. complete genome analysis revealed that the causative pathogen, tottori2, is a new pedv variant with a large (582 nt) deletion in the spike gene. phylogenetic analysis indicated that the tottori2 pedv strain might have been derived from the current pedv strains ...201526162305
evaluation of a commercial elisa for the detection of antibodies to sarcoptes scabiei in wild boar (sus scrofa).sarcoptic mange occurs in free-ranging wild boar (sus scrofa) but has been poorly described in this species. we evaluated the performance of a commercial indirect enzyme-linked immunosorbent assay (elisa) for serodiagnosis of sarcoptic mange in domestic swine when applied to wild boar sera. we tested 96 sera from wild boar in populations without mange history ("truly noninfected") collected in switzerland between december 2012 and february 2014, and 141 sera from free-ranging wild boar presentin ...201526161723
genome wide distributions and functional characterization of copy number variations between chinese and western pigs.copy number variations (cnvs) refer to large insertions, deletions and duplications in the genomic structure ranging from one thousand to several million bases in size. since the development of next generation sequencing technology, several methods have been well built for detection of copy number variations with high credibility and accuracy. evidence has shown that cnv occurring in gene region could lead to phenotypic changes due to the alteration in gene structure and dosage. however, it stil ...201526154170
submerged flapless technique vs. conventional flap approach for implant placement: experimental domestic pig study with 12-month follow-up.the aim of our study was to compare osseointegration and peri-implant crestal bone resorption in submerged flapless and conventional flap surgery over a 12-month follow-up.201626147852
alpha-crystallin-derived peptides as therapeutic chaperones.the demonstration of chaperone-like activity in peptides (mini-chaperones) derived from α-crystallin's chaperone region has generated significant interest in exploring the therapeutic potential of peptide chaperones in diseases of protein aggregation. recent studies in experimental animals show that mini-chaperones could reach intended targets and alter the disease phenotype. although mini-chaperones show potential benefits against protein aggregation diseases, they do tend to form aggregates on ...201526141743
validation of respiratory inductance plethysmography for measuring tidal volume in swine.measuring tidal volume (vt) in nonintubated swine or swine with leaking breathing circuits is challenging. the aim of this study was to validate respiratory inductance plethysmography (rip) for measuring vt in swine that are comparable in size to adult humans. to determine calibration curves, vt and rip readings were obtained from anesthetized swine (n = 8; weight, 46-50 kg) during positive-pressure (mechanical) ventilation and spontaneous breathing. for positive-pressure ventilation, 6 pigs wer ...026141447
optical coherence tomography of pulmonary arterial walls in humans and pigs (sus scrofa domesticus).pulmonary arterial hypertension (pah) is a devastating disorder characterized by progressive elevation of the pulmonary pressures that, in the absence of therapy, results in chronic right-heart failure and premature death. the vascular pathology of pah is characterized by progressive loss of small (diameter, less than 50 μm) peripheral pulmonary arteries along with abnormal medial thickening, neointimal formation, and intraluminal narrowing of the remaining pulmonary arteries. vascular pathology ...026141446
hepatitis e virus in domestic pigs, wild boars, pig farm workers, and hunters in estonia.while hepatitis e is a growing health concern in europe, epidemiological data on hepatitis e virus (hev) in estonia are scarce. along with imported hev infections, autochthonous cases are reported from european countries. both domestic and wild animals can be a source of human cases of this zoonosis. here, we investigated the presence of anti-hev antibodies and hev rna in domestic pigs and wild boars, as well as in pig farm workers and hunters in estonia. anti-hev antibodies were detected in 234 ...201526141050
cross-reactivity between aeroallergens and food allergens.in patients with respiratory allergy, cross-reactivity between aeroallergens and foods may induce food allergy, symptoms ranging from oral allergy syndrome to severe anaphylaxis. clinical entities due to ige sensitization to cross-reactive aeroallergen and food allergen components are described for many sources of plant origin (pollen-food syndromes and associations, such as birch-apple, cypress-peach and celery-mugwort-spice syndromes, and mugwort-peach, mugwort-chamomile, mugwort-mustard, ragw ...201526140270
allogenic ipsc-derived rpe cell transplants induce immune response in pigs: a pilot study.stem cell strategies focused on replacement of rpe cells for the treatment of geographic atrophy are under intense investigation. although the eye has long been considered immune privileged, there is limited information about the immune response to transplanted cells in the subretinal space of large animals. the purpose of this study was to evaluate the survival of allogenic induced pluripotent stem cell-derived rpe cells (ipsc-rpe) delivered to the subretinal space of the pig as well as determi ...201526138532
the porcine tspy gene is tricopy but not a copy number variant.the testis-specific protein y-encoded (tspy) gene is situated on the mammalian y-chromosome and exhibits some remarkable biological characteristics. it has the highest known copy number (cn) of all protein coding genes in the human and bovine genomes (up to 74 and 200, respectively) and also shows high individual variability. although the biological function of tspy has not yet been elucidated, its specific expression in the testis and several identified binding domains within the protein sugges ...201526133983
comparisons and uncertainty in fat and adipose tissue estimation techniques: the northern elephant seal as a case study.fat mass and body condition are important metrics in bioenergetics and physiological studies. they can also link foraging success with demographic rates, making them key components of models that predict population-level outcomes of environmental change. therefore, it is important to incorporate uncertainty in physiological indicators if results will lead to species management decisions. maternal fat mass in elephant seals (mirounga spp) can predict reproductive rate and pup survival, but no one ...201526121044
[18f]fdg accumulation in early coronary atherosclerotic lesions in pigs.inflammation is an important contributor to atherosclerosis progression. a glucose analogue 18f-fluorodeoxyglucose ([18f]fdg) has been used to detect atherosclerotic inflammation. however, it is not known to what extent [18f]fdg is taken up in different stages of atherosclerosis. we aimed to study the uptake of [18f]fdg to various stages of coronary plaques in a pig model.201526120829
chemical and metabolic profiling of si-ni decoction analogous formulae by high performance liquid chromatography-mass spectrometry.along with an indispensable role in healthcare system of china for centuries, traditional chinese medicine (tcm) shows increasing usages as complementary therapy in western countries. to improve our understanding on their therapeutic effects, it's critical to unveil chemical compositions of tcm formula, the predominant form of therapy in tcm. however, intrinsic chemical complexity makes it a challenging task to perform analysis on each individual tcm formula even with most current state-of-art a ...201526118924
electrophysiological, haemodynamic, and mitochondrial alterations induced by levobupivacaine during myocardial ischemia in a pig model: protection by lipid emulsions?accidental intravascular or high-dose injection of local anesthetics (la) can result in serious, potentially life-threatening complications. indeed, adequate supportive measures and the administration of lipid emulsions are required in such complications. the study's objectives were threefold: (i) evaluate the myocardial toxicity of levobupivacaine when administered intravenously; (ii) investigate levobupivacaine toxicity on cardiomyocytes mitochondrial functions and cellular structure; (iii) as ...201526118736
chinese border disease virus strain jsls12-01 infects piglets and down-regulates the antibody responses of classical swine fever virus c strain vaccination.during 2012 and 2013, several border disease virus (bdv) strains were identified from chinese goat and sheep herds. at the same time, pigs from the same areas were found to be seropositive to bdv by elisa, without showing clinical signs (unpublished data). to examine the susceptibility of pigs to the chinese bdv strains, bdv isolate jsls12-01, isolated from naturally infected sheep, was used to infect pigs. antibody responses, viremia, clinical signs and pathological changes of the infected anim ...201526117151
isolation and characterization of a novel rhabdovirus from a wild boar (sus scrofa) in japan.a novel rhabdovirus was isolated from the serum of a healthy japanese wild boar (sus scrofa leucomystax) and identified using the rapid determination system for viral nucleic acid sequences (rdv), next-generation sequencing, and electron microscopy. the virus was tentatively named wild boar rhabdovirus 1 (wbrv1). phylogenetic analysis of the entire genome sequence indicated that wbrv1 is closely related to tupaia rhabdovirus (trv), which was isolated from cultured cells of hepatocellular carcino ...201526116524
poly-d-l-lactic acid membranes for bone regeneration.to histologically and histomorphometrically evaluate the biocompatibility and the biological properties of perforated and nonperforated poly-d-l-lactic acid (pdlla) resorbable membranes, using a model of calvarial monocortical bone defects in domestic pigs.201526114511
vasopressin infusion with small-volume fluid resuscitation during hemorrhagic shock promotes hemodynamic stability and survival in swine.current management of hemorrhagic shock (hs) in the battlefield and civilian settings favors small-volume fluid resuscitation before controlling the source of bleeding. we investigated in a swine model of hs the effects of vasopressin infusion along with small-volume fluid resuscitation; with erythropoietin (epo) and hs severity as additional factors.201526107942
hepatitis e virus of subtype 3a in a pig farm, south-eastern france.hepatitis e virus (hev) has emerged during the past decade as a causative agent of autochthonous hepatitis and is a clinical concern in western developed countries. it has been increasingly recognized that pigs are a major reservoir of hev of genotypes 3 and 4 worldwide and pig-derived food items represent a potential source of infections by these viruses in humans. hepatitis e virus rna testing was performed here on faeces from rectal swabs sampled in 2012 from 50 3-month-old farm pigs from the ...201526102074
xromm analysis of tooth occlusion and temporomandibular joint kinematics during feeding in juvenile miniature pigs.like humans, domestic pigs are omnivorous and thus are a common model for human masticatory function. prior attempts to characterize food-tooth interactions and jaw movements associated with mastication have been limited to aspects of the oral apparatus that are visible externally (with videography) and/or to 2d movements of oral structures (with monoplanar videofluoroscopy). we used xromm, a 3d technique that combines ct-based morphology with biplanar videofluoroscopy, to quantify mandibular ki ...201526089531
the interactive effect of the degradation of cotton clothing and decomposition fluid production associated with decaying remains.textiles are a commonly encountered source of evidence in forensic cases. in the past, most research has been focused on how textiles affect the decomposition process while little attention has been paid to how the decomposition products interact with the textiles. while some studies have shown that the presence of remains will have an effect on the degradation of clothing associated with a decaying body, very little work has been carried out on the specific mechanisms that prevent or delay text ...201526074165
comparison of ketamine-dexmedetomidine-methadone and tiletamine-zolazepam-methadone combinations for short-term anaesthesia in domestic pigs.cardiorespiratory effects, quality of induction, depth of anaesthesia and quality of recovery were compared in pigs anaesthetised with 8 mg/kg ketamine, 20 µg/kg dexmedetomidine and 0.2 mg/kg methadone (kdm, n = 18) or 8 mg/kg tiletamine-zolazepam and 0.2 mg/kg methadone (tzm, n = 9). anaesthesia with kdm was partially reversed in nine animals with 0.2 mg/kg atipamezole (kdmat). sedation was observed earlier in the tzm group (47.2 ± 25.3 s) than the kdm group (91.5 ± 37.4 s). sternal and lateral ...201526070949
using genome-wide measures of coancestry to maintain diversity and fitness in endangered and domestic pig populations.conservation and breeding programs aim at maintaining the most diversity, thereby avoiding deleterious effects of inbreeding while maintaining enough variation from which traits of interest can be selected. theoretically, the most diversity is maintained using optimal contributions based on many markers to calculate coancestries, but this can decrease fitness by maintaining linked deleterious variants. the heterogeneous patterns of coancestry displayed in pigs make them an excellent model to tes ...201526063737
african swine fever virus georgia 2007 with a deletion of virulence-associated gene 9gl (b119l), when administered at low doses, leads to virus attenuation in swine and induces an effective protection against homologous challenge.african swine fever virus (asfv) is the etiological agent of an often lethal disease of domestic pigs. disease control strategies have been hampered by the unavailability of vaccines against asfv. since its introduction in the republic of georgia, a highly virulent virus, asfv georgia 2007 (asfv-g), has caused an epizootic that spread rapidly into eastern european countries. currently no vaccines are available or under development to control asfv-g. in the past, genetically modified asfvs harbor ...201526063424
genetic variation of the porcine nr5a1 is associated with meat color.because of the central role of steroidogenic factor 1 in the regulation of the development and function of steroidogenic tissues, including the adrenal gland, we chose the encoding gene nr5a1 as a candidate for stress response, meat quality and carcass composition in the domestic pig. to identify polymorphisms of the porcine nr5a1 we comparatively sequenced the coding, untranslated and regulatory regions in four commercial pig lines. single nucleotide polymorphisms could be found in the 3' utr a ...201626055431
expansion of the hsfy gene family in pig lineages : hsfy expansion in suids.amplified gene families on sex chromosomes can harbour genes with important biological functions, especially relating to fertility. the y-linked heat shock transcription factor (hsfy) family has become amplified on the y chromosome of the domestic pig (sus scrofa), in an apparently independent event to an hsfy expansion on the y chromosome of cattle (bos taurus). although the biological functions of hsfy genes are poorly understood, they appear to be involved in gametogenesis in a number of mamm ...201526055083
detection of porcine circovirus type 2 and its association with pmws in wild boars and domestic pigs in germany: a histopathological, immunohistochemical and molecular biological study.beside domestic pigs wild boars can also be affected by postweaning multisystemic wasting syndrome (pmws). for the first time a nationwide survey of wild boars (n = 356) and domestic pigs (n = 340) was carried out in germany by histopathology, immunohistochemistry (ihc) and quantitative pcr (qpcr). whereas 102/340 domestic pigs were immunoreactive for pcv2 antigen in at least one examined tissue, only 8/356 wild boars reacted positively. similar findings could be found in qpcr: all domestic pigs ...201526054225
[on the situation of african swine fever and the biological characterization of recent virus isolates].african swine fever (asf), a disease notifiable to the world organization of animal health (oie), is characterized by severe, unspecific clinical signs and high mortality rates. hosts for asf virus (asfv) are only members of the family suidae and soft ticks of the genus ornithodoros. currently, no vaccine is available and therefore, the control is primarily based on strict sanitary measures. the most important part is the early detection of the disease within affected animal holdings and the fas ...201526054220
evaluation of the zoonotic potential of a novel reassortant h1n2 swine influenza virus with gene constellation derived from multiple viral sources.in 2011-2012, contemporary north american-like h3n2 swine influenza viruses (sivs) possessing the 2009 pandemic h1n1 matrix gene (h3n2pm-like virus) were detected in domestic pigs of south korea where h1n2 siv strains are endemic. more recently, we isolated novel reassortant h1n2 sivs bearing the eurasian avian-like swine h1-like hemagglutinin and korean swine h1n2-like neuraminidase in the internal gene backbone of the h3n2pm-like virus. in the present study, we clearly provide evidence on the ...201526051886
intermediate veins in swine (sus scrofa domestica) kidney: authors' own anatomical classification.the use of domestic swine as an experimental animal is increasing steadily. swine organs are the best animal model for urological experiments. the aim of the study was to evaluate the course and size of intermediate veins in a swine kidney. the research results were compared with the results obtained from studies on venous vascularisation of human kidneys. the knowledge of the above-mentioned vessels is important both in human and veterinary medicine and will enable researchers to compare and no ...201526050812
anaplasma sp. and hemoplasma infection in leopard cats (prionailurus bengalensis euptilurus) from korea.this study examined the occurrence of anaplasma spp. and hemoplasma infection in leopard cats, prionailurus bengalensis euptilurus, in korea. twenty-nine biological samples were tested by molecular analysis. two (6.9%) and eight (27.6%) tested specimens were positive for anaplasma bovis and hemoplasma infection, respectively. based on our results, anaplasma/ehrlichia spp. and hemoplasma are regularly infecting leopard cat populations of korea. considering their endangered status, regular monitor ...201526040618
prevalence and characterization of influenza viruses in diverse species in los llanos, colombia.while much is known about the prevalence of influenza viruses in north america and eurasia, their prevalence in birds and mammals in south america is largely unknown. to fill this knowledge gap and provide a baseline for future ecology and epidemiology studies, we conducted 2 years of influenza surveillance in the eastern plains (los llanos) region of colombia. real-time reverse transcriptase polymerase chain reaction (rt-pcr) identified influenza viruses in wild birds, domestic poultry, swine a ...201326038461
carcass mass has little influence on the structure of gravesoil microbial communities.little is known about how variables, such as carcass mass, affect the succession pattern of microbes in soils during decomposition. to investigate the effects of carcass mass on the soil microbial community, soils associated with swine (sus scrofa domesticus) carcasses of four different masses were sampled until the 15th day of decomposition during the month of june in a pasture near lincoln, nebraska. soils underneath swine of 1, 20, 40, and 50 kg masses were investigated in triplicate, as well ...201626024793
vegf-c and vegf-c156s in the pro-lymphangiogenic growth factor therapy of lymphedema: a large animal study.vegf-c156s, a lymphangiogenesis-specific form of vascular endothelial growth factor c (vegf-c), has been considered as a promising candidate for the experimental pro-lymphangiogenic treatment, as it lacks potential angiogenic effects. as a precursor to future clinical trials, the therapeutic efficacy and blood vascular side effects of vegf-c and vegf-c156s were compared in a large animal model of secondary lymphedema. combination of lymphatic growth factor treatment and autologous lymph node tra ...201526018927
chest compression synchronized ventilation versus intermitted positive pressure ventilation during cardiopulmonary resuscitation in a pig model.guidelines recommend mechanical ventilation with intermitted positive pressure ventilation (ippv) during resuscitation. the influence of the novel ventilator mode chest compression synchronized ventilation (ccsv) on gas exchange and arterial blood pressure compared with ippv was investigated in a pig model.201526011525
east african pigs have a complex indian, far eastern and western ancestry.in this study, we have characterized the mitochondrial diversity of 81 swine from uganda. median-joining network analysis of d-loop sequences from these individuals and others characterized in previous studies allowed us to determine that ugandan pigs cluster with populations from the west (europe/north africa), far east and india. in addition, partial sequencing of the y-chromosome uty locus in 18 ugandan domestic pigs revealed the segregation of a single hy1 lineage that has a cosmopolitan dis ...201526011180
the synergy biodegradable polymer everolimus eluting coronary stent: porcine vascular compatibility and polymer safety study.synergy is a novel platinum chromium alloy stent that delivers abluminal everolimus from an ultrathin poly-lactide-co-glycide (plga) biodegradable polymer. this study evaluated the in vivo degradation of the polymer coating, everolimus release time course, and vascular compatibility of the synergy stent.201526009986
hepatitis e virus genotype 3 diversity: phylogenetic analysis and presence of subtype 3b in wild boar in europe.an increasing number of indigenous cases of hepatitis e caused by genotype 3 viruses (hev-3) have been diagnosed all around the word, particularly in industrialized countries. hepatitis e is a zoonotic disease and accumulating evidence indicates that domestic pigs and wild boars are the main reservoirs of hev-3. a detailed analysis of hev-3 subtypes could help to determine the interplay of human activity, the role of animals as reservoirs and cross species transmission. although complete genome ...201526008708
a quantitative ethnozoological study of traditionally used animals in pachamalai hills of tamil nadu, india.the purpose of this study was designed to gather primary folk knowledge on different animal based therapies used by malayalis in pachamalai hills. this is the first ethnozoological study in pachamalai hills; the data regarding the medicinal animals/animal products were documented and their usages were analyzed quantitatively.201526002766
first detection of sarcoptes scabiei from domesticated pig (sus scrofa) and genetic characterization of s. scabiei from pet, farm and wild hosts in israel.in this report we describe for the first time the detection of sarcoptes scabiei type suis mites on domestic pigs in israel and examine its genetic variation compared with s. sabiei from other hosts. microscopic examination of skin samples from s. scabiei-infested pigs (sus scrofa domesticus) revealed all developmental stages of s. scabiei. to detect genetic differences between s. scabiei from different hosts, samples obtained from pig, rabbits (orictolagus cuniculus), fox (vulpes vulpes), jacka ...201526002310
human microbiota-associated swine: current progress and future opportunities.gnotobiotic (gn) rodent models have provided insight into the contributions of the gut microbiota to host health and preventing disease. however, rodent models are limited by several important physiological and metabolic differences from humans, and many rodent models do not dependably replicate the clinical manifestations of human diseases. due to the high degree of similarity in anatomy, physiology, immunology and brain growth, the domestic pig (sus scrofa) is considered a clinically relevant ...201525991699
experimental pig-to-pig transmission dynamics for african swine fever virus, georgia 2007/1 strain.african swine fever virus (asfv) continues to cause outbreaks in domestic pigs and wild boar in eastern european countries. to gain insights into its transmission dynamics, we estimated the pig-to-pig basic reproduction number (r 0) for the georgia 2007/1 asfv strain using a stochastic susceptible-exposed-infectious-recovered (seir) model with parameters estimated from transmission experiments. models showed that r 0 is 2·8 [95% confidence interval (ci) 1·3-4·8] within a pen and 1·4 (95% ci 0·6- ...201625989921
dose-dependent vascular response following delivery of sirolimus via fast releasing, biodegradable polymer stent matrix: an experimental study in the porcine coronary model of restenosis.fast releasing, rapamycin-eluting stents, although safe, showed inferior results with regard to inhibition of restenosis.201525985728
genetic characterisation of porcine circovirus type 2 (pcv2) strains from feral pigs in the brazilian pantanal: an opportunity to reconstruct the history of pcv2 evolution.since its discovery, porcine circovirus type 2 has emerged as one of the most relevant swine infectious diseases, causing relevant economic losses for the pig industry. while four genotypes were identified, only three (pcv2a, pcv2b and pcv2d) are currently circulating and display a worldwide distribution. another genotype, pcv2c, has been described only once in danish archive samples collected between 1980 and 1990. in addition to commercial pigs, pcv2 has been demonstrated to infect wild boars ...201525975522
characterization of porcine parvovirus type 3 and porcine circovirus type 2 in wild boars (sus scrofa) in slovakia.as the number of free-living wild boars (sus scrofa l.) continues to rise in slovakia, the probability of pathogen transmission between susceptible species increases. we investigated the distribution and genetic characterization of porcine parvovirus type 3 (ppv3), porcine circovirus type 2 (pcv2), and their coinfection in wild boars. among 194 animals tested, 19.1% were positive for ppv3 and 43.8% for pcv2. similar rates of coinfection with both viruses reaching 11.0% and 11.8% were observed in ...201525973618
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