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winter survival of meloidogyne incognita in six soil types.winter survival of meloidogyne incognita in six soil types (fuquay sand, norfolk loamy sand, portsmouth loamy sand, muck, cecil sandy clay loam, and cecil sandy clay) was determined in microplots at one location from november 1981 to may 1982 and from november 1982 to march 1983. survival, based on second-stage juveniles (j2) of m. incognita, from november 1981 until may 1982 ranged from 1% in the muck soil to 6% in a cecil sandy clay loam, but survival rates were much higher the next year follo ...198819290193
interactions between meloidogyne incognita and pratylenchus brachyurus on soybean.interactions among meloidogyne incognita, pratylenchus brachyurus, and soybean genotype on plant growth and nematode reproduction were studied in a greenhouse. coker 317 (susceptible to both nematodes) and gordon (resistant to m. incognita, susceptible to p. brachyurus) were inoculated with increasing initial population densities (pi) of both nematodes individually and combined. m. incognita and p. brachyurus individually usually suppressed shoot growth of both cultivars, but only root growth on ...198819290187
histopathology of root-knot nematode (meloidogyne incognita) infection on white yam (dioscorea rotundata) tubers.white yam tissues naturally and artificially infected with root-knot nematodes were fixed, sectioned, and examined with a microscope. infective second-stage juveniles of meloidogyne incognita penetrated and moved intercellularly within the tuber. feeding sites were always in the ground tissue layer where the vascular tissues are distributed in the tubers. giant cells were always associated with xylem tissue. they were thin walled with dense cytoplasm and multinucleated. the nuclei of the giant c ...198819290181
production and partial characterization of stylet exudate from adult females of meloidogyne incognita.adult females of meloidogyne incognita were excised from tomato roots and incubated in 0.04 m phosphate buffered saline, ph 7.4 for 18-72 hours to allow accumulation of stylet exudate. twenty-four percent of the females produced exudate during the initial 18-hour incubation period; 70% of those females producing exudate initially produced additional exudate during the subsequent 54-hour incubation period. analysis of exudate by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed t ...198719290172
selective migration and root penetration by meloidogyne incognita and hoplolaimus columbus on soybean roots in vitro. 198719290161
sterol composition and ecdysteroid content of eggs of the root-knot nematodes meloidogyne incognita and m. arenaria.free and esterified sterols of eggs of the root-knot nematodes meloidogyne incognita races 2 and 3 and m. arenaria race 1 were isolated and identified by gas-liquid chromatography-mass spectrometry. the major sterols of eggs of each race were 24-ethylcholesterol (33.4-38.8% of total sterol), 24-ethylcholestanol (18.3-25.3%), 24-methylcholesterol (8.6-11.7%), 24-methylcholestanol (7.7-12.5%), and cholesterol (4.6-11.6%). consequently, the major metabolic transformation performed by meloidogyne fe ...198719290155
interaction between meloidogyne incognita and hoplolaimus columbus on davis soybean.greenhouse and laboratory experiments were performed to determine if an interaction exists between meloidogyne incognita and hoplolaimus columbus on davis soybean. greenhouse tests were performed with three population levels of m. incognita and h. columbus (0, 1,500, 6,000/1.5-liter pot) separately and in all combinations. dry root weight (drt) declined nonlinearly and dry shoot weight (dst) declined linearly with respect to increasing initial populations of m. incognita and h. columbus. when th ...198719290154
control of root-knot nematodes on tomato by lectins.significant control of tomato root knot was achieved by applications of the lectins concanavalin a (con a) and limax flavus agglutinin in greenhouse, growth chamber, and microplot trials. four consecutive weekly applications at lower concentrations of con a yielded better control than single applications at a higher total concentration. the present state of knowledge on binding of con a to soil nematodes and the in vitro effect of this lectin in chemotactic behavior are discussed. the mode of ac ...198719290152
effect of meloidogyne incognita on plant nutrient concentration and its influence on the physiology of beans.phaseolus vulgaris plants, 3, 8, 11, and 13 days old, were inoculated with 0, 2,000, 4,000, or 8,000 second-stage meloidogyne incognita larvae and maintained under controlled conditions. the photosynthetic rate and the shoot and root concentration of k, ca, mn, fe, cu, and zn were determined by destructive assay at 1-27-day intervals and by nondestructive assay of leaves, stems, and roots at 27 or 28 days after inoculation. in the destructive assay, the concentration of the elements in the plant ...198719290151
biological control of meloidogyne incognita by paecilomyces lilacinus and pasteuria penetrans.the root-knot nematode meloidogyne incognita was controlled more effectively and yields of host plants were greater when paecilomyces lilacinus and pasteuria penetrans were applied together in field microplots than when either was applied alone. yields of winter vetch from microplots inoculated with the nematode and with both organisms were not statistically different from yields from uninoculated control plots.198719290133
comparison of compatible and incompatible response of potato to meloidogyne incognita.one susceptible (d6) and two resistant (e2 and n4) clones of solanum sparsipilum x (s. phureja x haploid of s. tuberosum) were used to study the responses of potato roots and tubers to race 1 of meloidogyne incognita (kofoid &white) chitwood. the compatible response was characterized by rapid penetration of large numbers of second-stage juveniles (j2) into roots, cessation of root growth, and occasional curving of root tips. the life cycle of m. incognita in the susceptible clone was completed i ...198719290132
effective use of marine algal products in the management of plant-parasitic nematodes.algal extracts were ineffective against meloidogyne spp., panagrellus redivivus, and neoaplectana carpocapsae at 1.0% aqueous concentrations, with the exception of spatoglossum schroederi. s. schroederi killed meloidogyne incognita, m. javanica, m. acrita, and hoplolaimus galeatus at concentrations of 1.0, 0.75, and 0.50%. extracts from s. schroederi at a concentration of 1.0% were ineffective against hirschmanniella caudacrena and belonolaimus longicaudatus. spatoglossum schroederi, botryocladi ...198719290129
water absorbent polymer aids in the infestation of field sites with meloidogyne eggs. 198719290118
usefulness of egg assays in nematode population-density determinations. 198719290117
root-knot nematode management and yield of soybean as affected by winter cover crops, tillage systems, and nematicides.management of meloidogyne incognita on soybean as affected by winter small grain crops or fallow, two tillage systems, and nematicides was studied. numbers of m. incognita did not differ in plots planted to wheat and rye. yields of soybean planted after these crops also did not differ. numbers of m. incognita were greater in fallow than in rye plots, but soybean yield was not affected by the two treatments. soybean yields were greater in subsoil-plant than in moldboard plowed plots. ethylene dib ...198719290104
nicotine content of tobacco roots and toxicity to meloidogyne incognita.the motility of meloidogyne incognita second-stage juveniles (j2) and their ability to induce root galls in tomato were progressively decreased upon exposure to nicotine at concentrations of 1-100 mug/ml. ec values ranged from 14.5 to 22.3 mug/ml, but j2 motility and root-gall induction were not eliminated at 100 mug/ml nicotine. nicotine in both resistant nc 89 and susceptible nc 2326 tobacco roots was increased significantly 4 days after exposure to m. incognita. the increase was greater in re ...198719290102
relationship between morphology and parasitism in two populations of meloidogyne incognita.the reliability of morphological characters and host differential plants for distinguishing between two populations of meloidogyne incognita was studied. population a (originally from north carolina) had incognita-type perineal patterns. a single egg mass subpopulation of population a had a mixture of incognita and acrita perineal patterns with 33% of the patterns atypical for either species. population b (from georgia) had predominantly acrita-type patterns with only about 5% atypical patterns. ...198719290101
interaction of three plant-parasitic nematodes on corn and soybean.interaction of belonolaimus longicaudatus, meloidogyne incognita, and pratylenchus brachyurus on corn and b. longicaudatus, m. incognita, and heterodera glycines on soybean was investigated in micropiots during two seasons for corn and one season for soybean. changes in population densities and effects on plant growth of each nematode on corn or soybean alone and in mixed culture were compared. no interactions occurred on corn in 1987. in 1988, midseason population densities of b. longicaudatus ...199019287797
control of nematodes and soil-borne diseases in florida potatoes with aldicarb and 1,3-d.yields of u.s. size a potato tubers were increased by 1,3-dichloropropene (1,3-d) (56 liters/ha) and 1,3-d (56 liters/ha) + aldicarb (3.4 kg/ha) in 1982 and 1983 and by aldicarb (3.4 kg/ha) in 1982. treatments that included aldicarb provided the best control of corky ringspot disease, whereas those including 1,3-d were more effective against bacterial wilt. the most effective nematode control (nematodes included meloidogyne incognita, belonolaimus longicaudatus, trichodorids, criconemella ornata ...199019287795
effects of soil fumigants and aldicarb on nematodes, tuber quality, and yield in potato.efficacy of the fumigants ethylene dibromide (edb), edb + chloropicrin, and 1,3-dichloropropene (1,3-d) applied with one or three chisels per row, singly or in combination with aldicarb, was evaluated in 1982 and 1983 on potato (solanum tuberosum) cultivars atlantic and sebago for control of several nematodes, including belonolaimus longicaudatus, meloidogyne incognita, and trichodorids. generally, nematode populations were lowest following application of fumigants with three chisels per row, fo ...199019287794
reproduction of meloidogyne incognita on open-pollinated maize varieties.forty-three open-pollinated maize varieties were tested for resistance to the southern root-knot nematode, meloidogyne incognita race 4, in greenhouse tests. an experiment repeated on five different planting dates assessed nematode reproduction 60 days after inoculation with 3,000 eggs per plant. tebeau and old raccoon showed consistently high levels of resistance in all plantings, with the lowest reproduction factor (rf) values (0.2 and 0.4) and low numbers of eggs per gram of fresh root (222 a ...199019287774
spring or fall fumigation for control of meloidogyne spp. on tobacco.tests were conducted in 1987-88 to compare the efficacy of spring or fall fumigant nematicide applications for control ofmeloidogyne arenaria and m. incognita on tobacco. chloropicrin, 1,3-d, methyl isothiocyanate, and a methyl isothiocyanate-l,3-d mixture were applied as row treatments. fenamiphos, fenamiphos + fensulfothion, or ethoprop were applied in the spring as nonfumigant nematicide standards. fumigant nematicides increased yields and reduced galling (p = 0.01) in all four tests. spring ...199019287773
resistance in lycopersicon peruvianum to isolates of mi gene-compatible meloidogyne populations.root-knot nematode resistance of f progeny of an intraspecific hybrid (lycopersicon peruvianum var. glandulosum acc. no. 126443 x l. peruvianum acc. no. 270435), l. esculentum cv. piersol (possessing resistance gene mi), and l. esculentum cv. st. pierre (susceptible) was compared. resistance to 1) isolates of two meloidogyne incognita populations artificially selected for parasitism on tomato plants possessing the mi gene, 2) the wild type parent populations, 3) four naturally occurring resistan ...199019287762
identification of single meloidogyne juveniles by polymerase chain reaction amplification of mitochondrial dna.polymerase chain reaction (pcr) was used to amplify a specific 1.8-kb sequence of mitochondrial dna from single juveniles and eggs from 17 populations of meloidogyne incognita, m. hapla, m. javanica, and m. arenaria. approximately 2 mug amplified product were produced per reaction. restriction digestion of the amplified product with hinfi permitted discrimination of clonal lineages of the four species. meloidogyne javanica, however, could not be separated from m. hapla by the enzymes used in the ...199019287752
effects of planting date, small grain crop destruction, fallow, and soil temperature on the management of meloidogyne incognita.the effects of planting date, rye (secale cereale cv. wren abruzzi) and wheat (triticura aestivum cv. coker 797), crop destruction, fallow, and soil temperature on managing meloidogyne incognita race 1 were determined in a 2-year study. more m. incognita juveniles (j2) and egg-producing adults were found in roots of rye planted 1 october than in roots of rye planted 1 november and wheat planted 1 november and 1 december. numbers of m. incognita adults with and without egg masses were near or bel ...199019287731
response of resistant soybean plant introductions to meloidogyne incognita in field microplots.the response of two soybean plant introductions, pi 96354 and pi 417444, highly resistant to meloidogyne incognita, to increasing initial soil population densities (pi) (0, 31, 125, and 500 eggs/100 cm(3) soil) of m. incognita was studied in field microplots for 2 years. the plant introductions were compared to the cultivars forrest, moderately resistant, and bossier, susceptible to m. incognita. averaged across years, the yield suppressions of bossier, forrest, pi 417444, and pi 96354 were 97, ...199019287716
physiological response of resistant and susceptible vitis vinifiera cultivars to meloidogyne incognita.the effect of meloidogyne incognita on growth, general physiological response, and the concentration of reducing and nonreducing sugars at the nematode feeding sites of french colombard (susceptible) and thompson seedless (moderately resistant) vitis vinifiera cultivars was studied up to 2,100 degree-days (dd-base 10 c). nematode stress dosage, measured as the product of cumulative number of juveniles and females and their total energy (calories) demand, accounted for up to 15 and 10% of the ene ...199019287714
association of verticillium chlamydosporium and paecilomyces lilacinus with root-knot nematode infested soil.population densities of meloidogyne incognita and the nematophagous fungi, paecilomyces lilacinus and verticillium chlamydosporium, were determined in 20 northern california tomato fields over two growing seasons. paecilomyces lilacinus was isolated from three fields, v. chlamydosporium was isolated from one field, and both fungi were isolated from 12 fields. verticillium chlamydosporium numbers were positively correlated with numbers of m. incognita and p. lilacinus. paecilomyces lilacinus numb ...199019287711
meloidogyne incognita survival in soil infested with paecilomyces lilacinus and verticillium chlamydosporium.meloidogyne incognita-infected tomato seedlings were transplanted into sterilized soil or unsterilized soil collected from 20 california tomato fields to measure suppression caused by paecilomyces lilacinus, verticillium chlamydosporium, and other naturally occurring antagonists. unsterilized soils q, a, and h contained 35, 39, and 55% fewer m. incognita second-stage juveniles (j2) than did sterilized soil 1 month after infected tomato seedlings were transplanted to these soils and placed in a g ...199019287707
effects of cowpea and maize root leachates on meloidogyne incognita egg hatch. 199019287701
vertical distribution of plant-parasitic nematodes in sandy soil under soybean.vertical distribution of five plant-parasitic nematodes was examined in two north florida soybean fields in 1987 and 1988. soil samples were collected from 0-15 cm, 15-30 cm, and 30-45 cm deep at each site. soil at the three depths consisted of approximately 96% sand. more than 50% of belonolaimus longicaudatus population densities occurred in the upper 15-cm soil layer at planting, but the species became more evenly distributed through the other depths as the season progressed. criconemella sph ...199019287693
development, distribution, and host studies of the fungus macrobiotophthoira vermicola (entomophthorales).the life cycle and host range of macrobiotophthora vermicola were studied. secondary spores produced from forcibly ejected primary spores adhered to the cuticle of cruznema tripartitum, germinated, and penetrated the cuticle within 30 minutes. new primary spores were produced within 24 hours of initial spore adhesion. in a host range study, species of rhabditidae, diplogasteridae, and aphelenchoidea were hosts, but not species of bunonematidae, tripylidae, cephalobida, or tylenchina. numbers of ...199019287687
host suitability of soybean cultivars for meloidogyne incognita and m. arenaria.the suitability of five maturity group (mg) iii and five mg iv soybean, glycine max, cultivars as hosts for meloidogyne incognita and m. arenaria was evaluated in a greenhouse. 'forrest', a mg v cultivar, was used as the standard of comparison for m. incognita resistance. with m. incognita, root-gall and egg-mass indices and reproductive factors for 'asgrow 3307', 'ffr 398', and 'pioneer 9442' were comparable with those found on forrest. meloidogyne arenaria reproduction was lower (p </= 0.05) o ...198919287669
suitability of small grains as hosts of meloidogyne species.seven cultivars of wheat, five of oat, one of rye, and four of barley were tested as hosts for meloidogyne incognita, m. javanica, or m. arenaria under greenhouse conditions where soil temperature ranged from 21 to 34 c. reproduction rates of all nematode species were high on all cultivars, except m. javanica and m. arenaria on 'brooks' and 'florida 501' oat. meloidogyne incognita and m. javanica produced more eggs on roots of 'rutgers' tomato than on cultivars of wheat, oat, rye, or barley.198919287666
managing root-knot on tobacco in the southeastern united states.root-knot nematodes suppress yields of flue-cured tobacco an estimated 0.1 to 4.8% annually in the southeastern united states, even though nematode management practices have been widely adopted. although meloidogyne incognita races 1 and 3 have predominated, m. arenaria, m. javanica, and m. incognita races 2 and 4 are increasingly important. seventy-five percent of the flue-cured tobacco hectarage in north carolina and virginia is rotated on 2-year or 3-year intervals. over half of the hectarage ...198919287655
encapsulation of meloidogyne incognita eggs in carrageenan. 198919287650
drip irrigation as a delivery system for infestation of field plots with nematodes.a drip irrigation delivery system was used to infest field sites with the plant-parasitic root-knot nematodes, meloidogyne incognita. juvenile or egg inocula passed through the system without blockage of emitters or harm to the nematodes. field sites so infested were available for experimentation. delivery of approximately 5 x 10 to 10 juveniles or 10 to 3 x 10 eggs per emitter through the drip system resulted in heavy root galling of tomatoes planted next to the drip emitters. nematodes feeding ...198919287648
effects and dynamics of a nematode community on soybean.the relationships between densities of all members of a plant-parasitic nematode community and yield of 'davis' soybean and between final and preplant population levels were examined in small plots on sandy soils in north-central florida. plant-parasitic nematodes present in the community included belonolaimus longicaudatus, criconemella sphaerocephala, meloidogyne incognita, paratrichodorus minor, pratylenchus brachyurus, and xiphinema sp. plant growth, including stand count, soybean yield (kg/ ...198919287643
effect of carbamate, organophosphate, and avermectin nematicides on oxygen consumption by three meloidogyne spp.second-stage juveniles (i2) of meloidogyne arenaria consumed more oxygen (p </= 0.05) than m. incognita j2, which in turn consumed more than m. javanica j2 (4,820, 4,530, and 3,970 mul per hour per g nematode dryweight, respectively). decrease in oxygen consumption depended on the nematicide used. except for aldicarb, there was no differential sensitivity among the three nematode species. meloidogyne javanica had a greater percentage decrease (p </= 0.05) in oxygen uptake when treated with aldic ...198919287640
effects and dynamics of a nematode community on maize.relationships between nematode density and yield and between final and preplant population levels were examined in small maize plots on sandy soils in north-central florida. plant-parasitic nematodes present in the community included belonolaimus longicaudatus, criconemella sphaerocephala, meloidogyne incognita, paratrichodorus minor, pratylenchus brachyurus, and a xiphinema sp. plant growth--including stand count, grain yield, stalk weight, and size of young plants--often was inversely correlat ...198919287639
neoplastic growths in preparasitic juveniles of meloidogyne incognita. 198919287632
monoclonal antibodies to secretory granules in esophageal glands of meloidogyne species.monoclonal antibodies to secretory granules in the dorsal or subventral esophageal glands were generated by injecting balb/c mice with immunogens from preparasitic second-stage juveniles (j2) of meloidogyne incognita. antibodies specific for secretory granules in the j2 subventral esophageal glands or the dorsal gland were identified by indirect immunofluorescence microscopy. only antibodies that reacted with granules in the j2 dorsal gland reacted with the esophageal gland lobe ofm. incognita a ...198919287625
accelerated movement of nematodes from soil in baermann funnels with temperature gradients.baermann funnels were modified to eliminate or reverse the small temperature gradient (1-2 c/cm) across the soil layer that normally results from water evaporation. effects of modifications on extraction efficiency were examined at various ambient temperatures and after overnight adaptation of three nematode species at 20 and 30 c. extraction of meloidogyne incognita from sandy loam, tylenchulus semipenetrans from sandy clay loam, and rotylenchulus reniformis from silt was greatly accelerated si ...198919287622
endoparasitic nematodes in maize roots in the western transvaal as related to soil texture and rainfall.eight endoparasitic nematode species were recovered from 170 maize root samples in western transvaal, republic of south africa. pratylenchus zeae had the highest average population density (17,454/5 g roots), followed by p. neglectus (5,827/5 g roots), p. penetrans (5,617/5 g roots), p. brachyurus (3,060/5 g roots), meloidogyne incognita plus m. javanica (301 juveniles/5 g roots), p. crenatus (130/5 g roots), and rotylenchutus parvus (64/5 g roots). the 17 reasonably homogeneous farming areas (r ...198919287620
influence of environmental factors on the hatch and survival of meloidogyne incognita.the influence of soil temperature and moisture on meloidogyne incognita (kofoid and white) chitwood was examined in relation to hatching and survival of second-stage juveniles (j2). nematodes were cultured on cotton (gossypium hirsutum l. cv. acala sj2) under field conditions to provide populations similar to those found in the field in late autumn. egg masses were placed in a temperature range (9-12 c and 21 c), and hatch was measured over a period equivalent to 20 degree days > 10 c (dd10). ha ...198919287616
influence of four nematodes on root and shoot growth parameters in grape.two grape cultivars, susceptible french colombard and tolerant rubired, and four nematodes, meloidogyne incognita, pratylenchus vulnus, tylenchulus semipenetrans, and xiphinema index, were used to quantify the equilibrium between root (r) and shoot (s) growth. root and shoot growth of french colombard was retarded by m. incognita, p. vulnus, and x. index but not by t. semipenetrans. although the root growth of rubired was limited by all the nematodes, the shoot growth was limited only by x. inde ...198919287609
dna complexity of the root-knot nematode (meloidogyne spp.) genome.cot curves derived from renaturation kinetics of sheared denatured dna indicated that the genome of six populations representing the four most common root-knot nematode species (meloidogyne incognita, m. arenaria, m. javanica, and m. hapla) is composed of 20% repetitive and 80% nonrepetitive sequences of dna. cot curves were almost identical, indicating that all populations had a haploid genome of approximately the same size. calculations from an average cot curve gave an estimate of 0.51 x 108 ...198919287606
progression of root-knot nematode symptoms and infection on resistant and susceptible cottons.progressive development in cotton root morphology of resistant a623 and susceptible m-8 cotton (gossypium hirsutum l.) lines following infection by the root-knot nematode meloidogyne incognita was studied in glass front boxes. symptom development and radicle growth were observed; degree of galling, gall and egg mass diameter, and number of eggs per egg mass were recorded; and root segments were examined histologically. small cracks caused by m. incognita appeared in the root epidermis and cortex ...198919287602
root tissue response of two related soybean cultivars to infection by lectin-treated meloidogyne spp.treatment of second-stage juveniles (j2) of meloidogyne incognita race 1 and m. javanica with soybean agglutinin, concanavalin a, wheat germ agglutinin, lotus tetragonolobus agglutinin, or limax flavus agglutinin or the corresponding competitive sugars for each of these lectins did not alter normal root tissue response of soybean cultivars centennial and pickett 71 to infection by m. incognita race 1 or m. javanica. giant cells were frequently induced in centennial and pickett 71 roots 5 and 20 ...198919287600
growth of isolates of paecilomyces lilacinus and their efficacy in biocontrol of meloidogyne incognita on tomato.the potential of 13 paecilomyces lilacinus isolates from various geographic regions as biocontrol agents against meloidogyne incognita, the effects of temperature on their growth, and the characterization of the impact of soil temperature on their efficacy for controlling this nematode were investigated. maximum fungal growth, as determined by dry weight of the mycelium, occurred from 24 to 30 c; least growth was at 12 and 36 c. the best control of m. incognita was provided by an isolate from pe ...198919287594
structure-activity studies on nematicidal activity of dialkyl carbamates and thiocarbamates.in laboratory tests, 129 dialkyl carbamates of types roc(o)nhr', rsc(o)nhr', and roc(s)nhr' were tested in a screening bioassay against panagrellus redivivus. the 10 most active were lethal at concentrations from 5 ppm down to ca. 1 ppm. eight of these (the only ones active below 2.5 ppm) were thiolcarbamates (rsc(o)nhr'). decyl n-methyhhiolcarbamate was also lethal to meloidogyne incognita at approximately 1 ppm in direct contact tests.198919287593
in situ observations of root-gall formation using nuclear magnetic resonance imaging. 198919287587
survival of paecilomyces lilacinus in selected carriers and related effects on meloidogyne incognita on tomato.laboratory and microplot experiments were conducted to determine the influence of carrier and storage of paecilomyces lilacinus on its survival and related protection of tomato against meloidogyne incognita. spores of p. lilacinus were prepared in five formulations: alginate pellets (pellets), diatomaceous earth granules (granules), wheat grain, soil, and soil plus chitin. fungal viability was high in wheat and granules, intermediate in pellets, and low in soil and chitin-amended soil stored at ...198919287586
impact of paecilomyces lilacinus inoculum level and application time on control of meloidogyne incognita on tomato.microplot experiments were conducted to evaluate the effects of inoculum level and time of application of paecilomyces lilacinus on the protection of tomato against meioidogyne incognita. the best protection against m. incognita was attained with 10 and 20 g of fungus-infested wheat kernels per microplot which resulted in a threefold and fourfold increase in tomato yield, respectively, compared with tomato plants treated with this nematode alone. greatest protection against this pathogen was att ...198919287585
preferred temperature of meloidogyne incognita.in laboratory thermal gradients, newly hatched infective juveniles of the plant-parasitic root-knot nematode meloidogyne incognita migrated toward a preferred temperature that was several degrees above the temperature to which they were acclimated. after shifting egg masses to a new temperature, the preferred temperature was reset in less than a day. possible functions of this type of thermotaxis are discussed, including the use of thermal gradients around plant roots to locate hosts and to main ...198919287582
overwintering stages of meloidogyne incognita in vitis vinifera.the overwintering of meloidogyne incognita in and around vitis vinifera cv. french colombard roots was studied in a naturally infested vineyard at the kearney agricultural center, in a growth chamber, in inoculated vines in microplots at the university of california, davis, and in a greenhouse. infected roots were sampled at intervals from onset of vine dormancy until plants accumulated about 800 degree days (dd - base 10 c). embryogenesis within eggs, classified as less than or more than 16 cel ...198919287581
impact of meloidogyne incognita on physiological efficiency of vitis vinifera.four-week-old french colombard plants rooted from green cuttings were inoculated with 0, 1,000, 2,000, 4,000, or 8,000 meloidogyne incognita second-stage juveniles and maintained at 25 c night and 30 c day. leaf area and dry weight and the rates of photosynthesis, stomatal conductance, and internal leaf co concentration were measured at intervals up to 59 days after inoculation. nematode stress dosage, measured as the product of cumulative number of juveniles and females and their total energy ( ...198919287578
influence of glomus intraradices and soil phosphorus on meloidogyne incognita infecting cucumis melo.the interaction among glomus intraradices, meloidogyne incognita, and cantaloupe was studied at three soil phosphorus (p) levels in a greenhouse. all plants grew poorly in soil not amended with p, regardless of mycorrhizal or nematode status. in soil amended with 50 mug p /g soil, m. incognita suppressed the growth of nonmycorrhizal plants by 84%. in contrast, growth of mycorrhizal plants inoculated with m. incognita was retarded by only 21%. a similar trend occurred in plants grown in soil with ...198919287577
resistance to meloidogyne spp. in allohexaploid wheat derived from triticum turgidum and aegilops squarrosa.expression of resistance to meloidogyne incognita and m. javanica from aegilops squarrosa was studied in a synthetic allohexaploid produced from triticum turgidum var. durum cv. produra and ae. squarrosa g 3489. the reproductive rate of different races of m. incognita and m. javanica, expressed in eggs per gram of fresh root, was low (p < 0.05) on the synthetic allohexaploid and the resistant parent, ae. squarrosa g 3489, compared with different bread and durum wheat cultivars. reproduction of r ...198919287575
effects of rapeseed and vetch as green manure crops and fallow on nematodes and soil-borne pathogens.in a rapeseed-squash cropping system, meloidogyne incognita race 1 and m. javanica did not enter, feed, or reproduce in roots of seven rapeseed cultivars. both nematode species reproduced at low levels on roots of the third crop of rapeseed. reproduction of m. incognita and m. javanica was high on squash following rapeseed, hairy vetch, and fallow. the application of fenamiphos suppressed (p = 0.05) root-gall indices on squash following rapeseed, hairy vetch, and fallow; and on dwarf essex and c ...199219283212
counting nematodes with a microplate reader.the feasibility of counting plant-parasitic nematodes in aqueous suspensions by measuring light transmittance through aqueous suspensions with an elisa microplate reader was explored. absorbance readings for eggs or vermiform stages of three species were linearly related (r(2) > 0.99) to concentrations between 0 and 10,000 nematodes/ml. coefficients of variation ranged from 12-23%, depending on the species and developmental stage used. the method, therefore, was at least as accurate as direct co ...199219283207
parasitism of helicotylenchus lobus by pasteuria penetrans in naturally infested soil.the population density of helicotylenchus lobus and the percentage of the population with spores of pasteuria penetrans were determined for 10 monthly intervals in naturally infested turf grass soil at riverside, california. the percentage of nematodes with attached spores ranged from 40% to 67%. no relationship was found between nematode density and the percentage of nematodes with spores. the mean and maximum numbers of spores adhering per nematode with at least one spore ranged from 2 to 8 an ...199219283198
invasion of tomato roots and reproduction of meloidogyne incognita as affected by raw sewage sludge.the antagonistic effects of raw sewage sludge on infection of tomato by meloidogyne incognita were tested in greenhouse pot experiments. sludge was mixed with the soil or added on its surface before and after inoculation of tomato plants with nematode eggs. juvenile penetration was determined 1 and 10 days after inoculation, and 6 weeks later root systems were assessed for nematode reproduction. fewer juveniles penetrated roots in pots with sludge added to the soil than in unamended control pots ...199119283192
evaluation of two soybean cultivars and aldicarb treatment in soil infested with plant-parasitic nematodes.the soybean cultivars davis and leflore were grown in a northern florida experimental site infested with eight species of plant-parasitic nematodes. meloidogyne incognita appeared to become the predominant pathogen, suppressing davis soybean yield regardless of aldicarb treatment. soybean yields of leflore were 1.4-fold to 3.5-fold greater than yields of davis, but aldicarb did not affect yields of either cultivar. davis soybean yield was negatively correlated with the at-plant density of m. inc ...199119283184
nematode population changes and forage yields of six corn and sorghum cultivars.two temperate corn (zea mays) hybrids (pioneer 3320 and northrup king 508), two tropical corn cultivars (pioneer x304c hybrid and florida syn-1 experimental open pollinated cultivar), the sorghum (sorghum bicolor) x sudangrass (sorghum sudanense) hybrid dekalb sx-17, and the sorghum hybrid dekalb fs25e were compared for effect on nematode densities and forage yield in three plantings (one single-crop and one double-crop system) in florida. final population densities of meloidogyne incognita in t ...199119283183
nematode densities associated with corn and sorghum cropping systems in florida.final densities (pf) of meloidogyne incognita and pratylenchus spp. increased more than ten-fold over initial densities (pi) on corn (zea mays) cultivars grown at three sites in north florida. the pf of m. incognita following sorghum (sorghum bicolor) were much lower (p </=0.001) than pf following corn at the two sites in which sorghum was grown. at one of these sites, pf of m. incognita was </= 5/100 cm(3) soil, and at the other site pf < pi. at one site, population densities of criconemella sp ...199119283182
meloidogyne arenaria populations on soybean.the distribution of meloidogyne spp. was determined in the piedmont and coastal plains soybean production areas of south carolina. meloidogyne arenaria, m. incognita, and m. javanica were found in six of seven counties surveyed, with some populations consisting of two or more species. because m. arenaria populations did not reproduce on peanut (arachis hypogaea cv. florunner), they were designated as host race 2. severity of root galling, shoot and root growth, seed yield, and nematode reproduct ...199119283177
race composition of meloidogyne incognita and m. arenaria populations in vegetable fields in uttar pradesh.a total of 1,256 populations of meloidogyne incognita and 442 populations of m. arenaria were collected from vegetables in eight districts of uttar pradesh, india. host differentials were used to identify the host race of each population. all four host races of m. incognita were present in six of the eight districts. in the other two districts, only host races 1, 2, and 4 were found. although frequencies of occurrence of the races differed among districts, races 1 and 2 comprised 62% of all m. i ...199119283172
resistance to root-knot nematodes in cucumber and horned cucumber.two experiments were conducted in the greenhouse. in one experiment, cucumber (cucumis sativus) and horned cucumber (c. metuliferus) cultigens were evaluated for resistance to four root-knot nematode species (meloidogyne arenaria, m. hapla, m. incognita, and m. javanica), and, in a second experiment, a standard (12-week) test was compared with a rapid (6-week) test. in the first experiment, horned cucumber cultigens varied in response to the meloidogyne species. 'sumter' cucumber was more suscep ...199119283171
response of tomato cultigens to meloidogyne javanica and races of meloidogyne incognita.thirty-six cultigens of tomato were evaluated for resistance against meloidogyne javanica and four races of m. incognita with standards and parameters adopted by the international meloidogyne project. most cultigens were susceptible to the nematodes, including some that were previously reported to be resistant to these nematodes. ten accessions, namely pusa-120, calmart vfn, panjab 6.nr-7, ec173898 (72t6), ec173897 (cal-mart), ec173896 (kewalo), cln363bcf-167-1-0, cln363bcf-190-1-0, cln363bcf-34 ...199119283169
reaction of trifolium repens cultivars and germplasms to meloidogyne incognita.ten cultivars and 13 germplasms of white clover (trifolium repens) were evaluated in the greenhouse for resistance to the southern root-knot nematode, meloidogyne incognita race 4. one hundred plants of each cultivar or germplasm were rated for percentage of the root system galled (prsg) at 60 days after inoculation with root-knot nematode eggs. tillman (9%) and srvr (19%) had the highest percentage of resistant plants (prsg = 0 or 1 on a scale of 0-5 ) for the cultivars and germplasms, respecti ...199119283168
resistance in soybean cultivars from maturity groups v-viii to soybean cyst and root-knot nematodes.one hundred thirty-nine cultivars of soybean (glycine max) in maturity groups v, vi, vii, and viii were evaluated in a greenhouse for resistance to heterodera glycines races 3 and 14 and meloidogyne incognita, m. arenaria, and m. javanica. of the cultivars tested, 37% had resistance to h. glycines race 3 alone, 12% had resistance to both races 3 and 14, and 69% exhibited a moderate or high level of resistance to one or more of the meloidogyne spp. however, 24% were susceptible to each race of h. ...199119283166
reaction of prunus rootstocks to meloidogyne incognita and m. arenaria in spain.prunus rootstocks were evaluated for their reaction to meloidogyne incognita and m. arenaria. most rootstocks were peach-almond hybrids of spanish origin. in one experiment three selections of garfi x nemared (g x n) and hansen-5 were highly resistant to m. incognita, but four other rootstocks were susceptible showing high galling indices and population increases. in two experiments with m. arenaria, the hybrid selections g x n nos. 1 and 9 were immune, gf-305 and hansen-5 were resistant, but ni ...199119283164
interaction of meloidogyne incognita and water stress in two cotton cultivars.a series of controlled-environment experiments were conducted to elucidate the effects of meloidogyne incognita on host physiology and plant-water relations of two cotton (gossypium hirsutum) cultivars that differed in their susceptibility to nematode infection. inoculation of m. incognita-resistant cultivar auburn 634 did not affect growth, stomatal resistance, or components of plant-water potential relative to uninoculated controls. however, nematode infection of the susceptible cultivar stone ...199119283156
comparisons of isozyme phenotypes in five meloidogyne spp. with isoelectric focusing.meloidogyne incognita race 1, m. javanica, m. arenaria race 1, m. hapla, and an undescribed meloidogyne sp. were analyzed by comparing isozyme phenotypes of esterase, malate dehydrogenase, phosphoglucomutase, isocitrate dehydrogenase, and alpha-glycerophosphate dehydrogenase. isozyme phenotypes were obtained from single mature females by isoelectric focusing electrophoresis. of these five isozymes, only esterase and phosphoglucomutase could be used to separate all five meloidogyne spp.; however, ...199119283155
infection of cultured thin cell layer roots of lycopersicon esculentum by meloidogyne incognita.a new aseptic culture system for studying interactions between tomato (lycopersicon esculentum) and meloidogyne incognita is described. epidermal thin cell layer explants from peduncles of tomato produced up to 20 adventitious roots per culture in 4-9 days on murashige &scoog medium plus kinetin and indole acetic acid. rooted cultures were transferred to gamborg's b-5 medium and inoculated with infective second-stage juveniles. gall formation was apparent 5 days after inoculation and egg product ...199119283152
evidence against amplification of four genes in giant cells induced by meloidogyne incognita.giant-cell dna was isolated from pea (pisum sativum) inoculated with meloidogyne incognita and used in slot blots to test for selective sequence amplification. four sequences representing low (ribulose 1,5-bisphosphate carboxylase and actin), mid-level (histone 3), and highly repetitive (large ribosomal repeat) sequence dna were used as probes. known amounts of root-tip dna and giant-cell dna were blotted onto hybridization membranes and probed. the signal strength on autoradiographs containing ...199119283148
plant protection with inorganic ions.gradients of salts of the specific ion repellents for meloidogyne incognita -- nh, k, cl, and no -- have been demonstrated to shield tomato roots from infestation in soil. the strategy of these greenhouse experiments was to interpose a salt barrier in a soil column between the plant roots and the nematodes. the relative effectiveness of the salts as a barrier to infective second-stage juveniles in a sandy loam was nhno, nhcl > kno > kcl. some of these ions are beneficial to plant growth, and the ...199119283146
yield-loss models for tobacco infected with meloidogyne incognita as affected by soil moisture.yield-loss models were developed for tobacco infected with meloidogyne incognita grown in microplots under various irrigation regimes. the rate of relative yield loss per initial nematode density (pi), where relative yield is a proportion of the value of the harvested leaves in uninfected plants with the same irrigation treatment, was greater under conditions of water stress or with high irrigation than at an intermediate level of soil moisture. the maximum rate of plant growth per degree-day (b ...199119283140
isolation of a repeated dna probe showing polymorphism among meloidogyne incognita populations.several meloidogyne incognita geographic populations were characterized by analysis of the restriction fragment length polymorphisms (rflp) obtained after digestion of their total dna and hybridization with a [(3)(2)p]-labeled probe. the probe consisted of a 1.7-kb-repeated dna sequence, isolated from a m. incognita genomic library, that hybridized to multiple bamh i fragments in the genome of each isolate. the patterns showed sufficient polymorphism to enable the accurate differentiation of all ...199119283132
interaction of meloidogyne javanica with different races of meloidogyne incognita.the interspecific interactions of meloidogyne javanica with races 1, 2, 3, and 4 of m. incognita on tomato were determined. impacts of the interactions on fecundity and morphometrics of females were also examined. mutually inhibitory interactions occurred between m. javanica and the races of m. incognita, but the negative interactions did not reflect in plant growth. numbers of root galls, egg masses, mature females, total population, fecundity, and reproduction factor declined in concomitant tr ...199119283130
biomass partitioning in tomato plants infected with meloidogyne incognita.tomato plants were inoculated with meloidogyne incognita at initial populations (pi) of 0, 1, 10, 50, 100, and 200 (x 1,000) eggs per plant and maintained in a growth chamber for 40 days. total fresh biomass (roots + shoots) at harvest was unchanged by nematode inoculation with pi of 1 x 10 eggs or less. reductions in fresh shoot weight with increasing pi coincided with increases in root weight. total fresh biomass declined with pi above 1 x 10 eggs, whereas total dry biomass declined at pi abov ...199119283129
characteristics and efficacy of a sterile hyphomycete (arf18), a new biocontrol agent for heterodera glycines and other nematodes.a filamentous, nonsporulating fungus, designated arkansas fungus 18 (arf18), was isolated from 9 of 95 populations of heterodera glycines, the soybean cyst nematode, in arkansas. in petri dishes, arf18 parasitized 89% of h. glycines eggs in cysts. the fungus also infected eggs of meloidogyne incognita and eggs in cysts of cactodera betulae, h. graminophila, h. lespedezae, h. leuceilyma, h. schachtii, and h. trifolii. in pot tests, reproduction of scn was 70% less in untreated field soil that was ...199119283127
stage-specific differences in lectin binding to the surface of anguina tritici and meloidogyne incognita.the occurrence and distribution of several lectin binding sites on the outer surfaces of eggs, preparasitic second-stage juveniles (j2), parasitic second-stage juveniles (pj2), females, and males of two tylenchid nematodes, anguina tritici and meloidogyne incognita race 3, were compared. in both species, a greater variety of lectins bound to the eggs than to other life stages; lectin binding to eggs was also more intense than it was to other life stages. species-specific differences also occurre ...199119283123
induction of isoperoxidases in resistant and susceptible tomato cultivars by meloidogyne incognita.isoperoxidases were detected in resistant rossol and susceptible roma vf tomato roots uninfected and infected by meloidogyne incognita. syringaldazine, guaiacol, p-phenylenediamine-pyrocatechol (ppd-pc), and indoleacetic acid (iaa) were used as substrates, and the corresponding peroxidative activities were detected either in cytoplasmic or in cell wall fractions, except for iaa oxidase, which was measured in soluble and microsomal fractions. isoperoxidase activities and cellular locations were i ...199119283122
size differences among root-knot nematodes on resistant and susceptible alyceclover genotypes.the influence of plant resistance on the size of individual root-knot nematodes was determined in greenhouse experiments. five genotypes of alyceclover were inoculated with second-stage juveniles of meloidogyne incognita race 3 or m. arenaria race 1. plants were harvested at selected intervals and stained for detection of the nematodes, which were dissected from the roots. length, width, and sagittal-sectional area of each animal were measured using an image-analysis system, and areas of nematod ...199119283120
interaction between meloidogyne incognita and agrobacterium tumefaciens or fusarium oxysporum f. sp. lycopersici on tomato.agrobacterium tumefaciens stimulated and fusarium oxysporum f. sp. lycopersici inhibited development and reproduction of meloidogyne incognita when applied to the opposite split root of tomato, lycopersicon esculentum cv. tropic, plants. the lowest rate of nematode reproduction occurred after 2,000 juveniles were applied and the fungus was present in the opposite split root. the effects of all three pathogens alone on the growth of roots and shoots of tomato plants were evident, but m. incognita ...199119283119
penetration of susceptible and resistant tobacco cultivars by meloidogyne juveniles.rates of penetration of meloidogyne incognita, m. arenaria, and m. javanica into tobacco cultivars nc2326 (susceptible to all three species) and k399 (resistant to m. incognita) and a breeding line that had been selected for resistance to m. incognita were compared. meloidogyne incognita penetrated nc2326 rapidly during the first 24 hours after inoculation. numbers of m. incognita continued to increase gradually through the 14-day experiment. higher numbers of m. incognita were observed in the r ...199119283116
modification of resistance expression of phaseolus vulgaris to meloidogyne incognita by elevated soil temperatures.the effect of temperature on the reaction of susceptible (canario divex) and resistant (a 211) bean pure lines to meloidogyne incognita was studied with soil temperature tanks housed in a growth chamber at 22 or 24 c. soil temperature remained constant at 16, 22, 24, 26, 30, or 32 c in several trials. bean line a 211 was resistant at 16 and 22 c but was susceptible at 24 c and above. resistance to root-knot nematode reproduction was affected by a lower temperature (24 c) than was resistance to r ...199119283110
biological control of soil pests by mixed application of entomopathogenic and fungivorous nematodes.in greenhouse experiments, massive application of the fungivorous nematode, aphelenchus avenae, in summer at 26-33 c (1 x l0 nematodes/500 cm(3) autoclaved soil) or in autumn at 18-23 c (5 x 10 nematodes/500 cm(3) autoclaved soil) suppressed pre-emergence damping-off of cucumber seedlings due to rhizoctonia solani ag-4 by 67% or 87%, respectively. application of 2 x l0 a. avenae to sterilized soil infested with r. solani caused leafminer-like symptom on the cotyledons, which did not occur in mix ...199119283109
penetration and development of meloidogyne incognita on roots of resistant soybean genotypes.meloidogyne incognita penetration and development were studied in roots of highly resistant (pi 96354, pi 417444), resistant (forrest), and susceptible (bossier) soybean genotypes. although more second-stage juveniles (j2) had penetrated roots of pi 96354 and pi 417444 than roots of forrest and bossier by 2 days after inoculation, fewer j2 were present in roots of pi 96354 at 4 days after inoculation. juvenile development in all genotypes was evident by 6 days after inoculation, with the highest ...199119283106
relationships between soil and levels of meloidogyne incognita and tobacco yield and quality.a 2-year study with six soils and four levels of meloidogyne incognita in microplots was designed to determine the effects of these parameters on nematode activity and tobacco yield and quality. key components under study were affected by soil, nematode level, and season (year-cultivar). in 1980, low initial nematode numbers (1,250) enhanced tobacco yield in cecil clay loam, but caused slight to moderate yield losses in the other soils. yield losses to m. incognita were generally greatest in san ...199119283097
survey of plant-parasitic nematodes in missouri cotton fields.during september 1990, 30 cotton fields in each of three missouri counties were surveyed for plant-parasitic nematodes. soil samples for nematode analysis consisted of a composite of 20 cores collected in a zig-zag pattern within a 1-ha block in each field. cores were taken from within weed-free cotton rows. nine genera of plant-parasitic nematodes were found (rotylenchulus, helicotylenchus, hoplolaimus, meloidogyne, paratylenchus, pratylenchus, tylenchorhynchus, heterodera, and trichodorus), an ...199219283062
survey of nematodes on coffee in hawaii.surveys of coffee fields in hawaii during 1989-1991 indicated the presence of 10 nematode species in 8 genera. after coffee was planted in fields previously in sugarcane, populations of criconemella sp. and pratylenchus zeae gradually decreased, while rotylenchulus reniformis and, in one field, meloidogyne incognita, increased in numbers. coffee is a poor host of r. reniformis, but weeds in coffee plantations may support this nematode. at present, nematodes pose no serious threat to hawaii's exp ...199219283060
occurrence of meloidogyne spp. in argentina.a record of 84 plant species in 32 families that are hosts to the root-knot nematode species found in argentina is presented. the genus meloidogyne appears to be widely distributed in the country, with meloidogyne incognita and m. javanica the most frequently detected species. other species found in argentina include m. arenaria, m. cruciani, m. decalineata, m. hapla, and m. ottersoni. the present survey is supplemented with existing published information.199219283059
host suitability of ixora spp. for the root-knot nematodes meloidogyne incognita race 1 and m. javanica.eight commonly cultivated ixora species or cultivars were tested for their suitability as hosts and their level of tolerance to meloidogyne incognita race 1 and m. javanica in a greenhouse study. twenty weeks postinoculation with 5,000 eggs per pot, m. incognita race 1 and m. javanica produced galls and formed egg masses on roots of all eight ixora species or cultivars tested. however, only m. javanica-infected 'petite yellow' and 'maui' had decreases (p </= 0.05) in root wet weights, suggesting ...199219283052
reproduction of four races of meloidogyne incognita on hibiscus cannabinus.the feasibility of cultivation of kenaf (hibiscus cannabinus) in the united states is receiving a multifaceted evaluation. among the factors being evaluated is kenafs susceptibility to nematodes. in this investigation, four races of meloidogyne incognita reproduced extensively on each of the several kenaf genotypes examined in greenhouse tests. some genotypes of kenaf, however, demonstrated limited resistance to certain races of m. incognita.199219283051
effects of root decay on the relationship between meloidogyne spp. gall index and egg mass number in cucumber and horned cucumber.a greenhouse study was conducted to determine if root necrosis had an effect on the relationship between root-knot nematode gall index and egg mass number. thirty-four cultigens of cucumis (14 accessions, 12 cultivars, and six breeding lines of c. sativus, and two accessions of c. metuliferus) were evaluated against four root-knot species (meloidogyne arenaria race 2, m. incognita race 1, m. incognita race 3, and m. javanica) measuring gall index, root necrosis, and egg mass number. root necrosi ...199219283049
comparison of nematode population densities on six summer crops at seven sites in north florida.densities of plant-parasitic nematodes were compared on six crops grown for forage during the summer of 1991 at seven sites in north central florida. the cropping treatments were 'howard' soybean (glycine max), 'deltapine 105' soybean, velvetbean (mucuna deeringiana), 'california blackeye #5' cowpea (vigna unguiculata), 'pioneer 3098' tropical corn (zea mays), and 'asgrow chaparral' sorghum (sorghum bicolor). highest final densities (pf) of meloidogyne incognita and criconemella spp. were obtain ...199219283048
establishment of orchards with black polyethylene film mulching: effect on nematode and fungal pathogens, water conservation, and tree growth.placement of a 3-m-wide, black, polyethylene film mulch down rows of peach (prunus persica 'red haven' on 'lovell' rootstock) and almond (prunus dulcis 'nonpareil' on 'lovell') trees in the san joaquin valley of california resulted in irrigation water conservation of 75%, higher soil temperature in the surface 30 cm, a tendency toward greater root mass, elimination of weeds, and a greater abundance of meloidogyne incognita second-stage juveniles in soil but reduced root galling when compared to ...199219283045
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