Publications

a serodiagnostic assay by microenzyme-linked immunosorbent assay for human anisakiasis using a monoclonal antibody specific for anisakis larvae antigen.	a serodiagnostic assay system to detect human anisakiasis is described. this assay uses a microenzyme-linked immunosorbent assay with monoclonal antibody an2 that recognizes the antigen specifically found in anisakis simplex larvae. the data showed that sera from infected patients reacted strongly with the a. simplex larvae antigen that was immobilized with monoclonal antibody an2 on microplates. patients' igg, iga, igm, and ige reacted strongly with an2-defined antigen at 4 or 5 weeks and at 1 ...	1990	2324550
anisakis simplex: histopathological changes in experimentally infected cba/j mice.	third-stage juveniles (l3) of anisakis simplex, surgically implanted into the abdominal cavity of cba/j mice and necropsied at 7, 14, or 21 days postinfection (pi), embedded in the gut mesentery and only rarely invaded viscera. histologically, intense aggregations of neutrophils adjacent to the parasites were noted at day 7 pi. at day 14 pi, mature granulomata consisting mostly of eosinophils and large numbers of fibroblasts and associated collagen were observed. granulocytes and occasionally mu ...	1990	2311712
immunoblot analysis of serum igg, iga and ige responses against larval excretory-secretory antigens of anisakis simplex in patients with gastric anisakiasis.	to increase our understanding of the immune response to anisakis infection, antigen specific igg, iga and ige responses were identified using an immunoblot technique after polyacrylamide gel electrophoresis of excretory-secretory products from the larval stage of anisakis simplex. nine sera were drawn from proven cases of gastric anisakiasis within 3 days after symptoms had developed. the molecular weight of the major antigenic bands were distributed between 50 kda and 120 kda of the antigens. i ...	1990	2283472
identification of the secreted neutral proteases from anisakis simplex.	ingestion of larval nematodes (family: anisakidae) can cause the human disease known as anisakiasis. after ingestion, anisakis larvae can be invasive, penetrating host stomach or intestinal wall. observation of larvae penetrating the tissue layers of human stomach in vitro by sem showed tunnels and burrows were formed in the mucosa and submucosa. based on these observations, we hypothesized that secreted proteases may be involved in the degradation of host tissue macromolecules to allow tunnel f ...	1990	2213405
human anisakiasis transmitted by marine food products.	seafood-transmitted parasitic diseases represent an emerging area of interest to the u.s. food and drug administration. human infections with marine parasites are generally the result of ingesting uncooked seafood products. over 50 species of helminthic parasites are known to infect humans worldwide. recently, the number of infections with one of these helminths, the juvenile stage of the marine nematode, anisakis simplex, has increased in the united states. raw fish dishes such as lomi lomi sal ...	1991	2022472
anisakis simplex, a co-factor of gastric cancer?		1990	1976977
[seroepidemiological data on anisakiasis: prophylactic consequences in fish products].	the western blotting method allowed us to confirm the diagnosis in 7 cases of acute anisakiasis, to assess it in 5 cases of chronic anisakiasis, and to follow up the evolution. these chronic frames according to the question of their long term evolution, and the part played by anisakis simplex in fish nutritional allergy increase the negative score of that parasite. this situation should leave to discuss preventive rules.	1991	1863866
[detection of antibodies against the larva of anisakis simplex in the pollock pollachius virens using elisa].	from the fact that older saithes (more than 5 years old) are showing significantly lower prevalence of attack by anisakis larvae in lateral muscle than younger saithes (3-4 years old), the question arises if this phenomenon is based on a specific immune response. therefore we have investigated serum samples from saithes of different ages by using an indirect elisa to estimate the antibody-titer against excretory-secretory anisakis antigen. results showing a moderate correlation (r = 0.66) betwee ...	1991	1858459
changes of igm and igg antibody levels in experimental rabbit anisakiasis as observed by elisa and sds-page/immunoblot.	antibody changes in experimental anisakiasis were observed in 10 rabbits which were infected each with 10 anisakis simplex larvae. the sera were collected before and on the 6th to the 95th day after the infection. using crude saline extract of anisakis larvae as antigen, specific igm and igg antibody levels were observed by elisa and sds-polyacrylamide gel electrophoresis/immunoblot. levels of specific-igm antibody were elevated from the 6th day, reached their peaks on the 11th day after the inf ...	1991	1804303
anisakidae larvae found in marine fishes and squids from the gulf of tongking, the east china sea and the yellow sea.	marine fishes and squids caught in the gulf of tongking, the east china sea and the yellow sea were dissected, and many anisakidae larvae were found in them. the larvae of anisakis simplex (anisakis type i larvae), the most important causative agent of anisakiasis, was found in about 30% of fishes and squids caught in the gulf of tongking and in about 60% of those caught in the east china sea and the yellow sea. larvae belonging to three other genera than anisakis--raphidascaris or raphidascaroi ...	1991	1784084
[seroepidemiologic data on anisakiasis. prophylactic consequences for fish products].	the western blotting method allowed us to confirm the diagnosis in 7 cases of acute anisakiasis, to assess it in 5 cases of chronic anisakiasis, and to follow up the evolution. these chronic frames according to the question of their long term evolution, and the part played by anisakis simplex in fish nutritional allergy increase the negative score of that parasite. this situation should lead to discuss preventive rules.	1991	1772241
biological tags for stock separation in pacific herring clupea harengus pallasi in california.	a survey of the parasites of pacific herring (clupea harengus pallasi) off northern california identified 1 species of acanthocephala, 1 species of cestoda, 2 species of copepoda, 1 species and 1 family of digenea, 3 species of nematoda, and 3 species of protozoa. from this survey, lacistorhynchus dollfusi (cestoda), parahemiurus merus (digenea), and anisakis simplex, contracaecum sp., hysterothylacium sp. (nematoda) were selected as potential tags. herring were collected in tomales, san francis ...	1992	1738068
gastrointestinal helminths of the cetaceans phocoena spinipinnis (burmeister, 1865) and cephalorhynchus eutropia (gray, 1846) from the southern coast of chile.	gastrointestinal helminths of the cetaceans cephalorhynchus eutropia and phocoena spinipinnis accidentally entangled in gillnets off the coast of queule, chile, were identified from 1989 to 1990. pseudoterranova sp., polymorphus (polymorphus) cetaceum and synthesium tursionis occurred in both cetaceans. additionally, anisakis sp. and braunina cordiformis were found in c. eutropia, and anisakis simplex was identified from p. spinipinnis. the species with the highest prevalence and mean intensity ...	1992	1602588
a case of abdominal syndrome caused by the presence of a large number of anisakis larvae.	a 58-year-old woman living in shizuoka prefecture, japan, complained of severe epigastric pain and nausea about 8 h after having eaten 'sashimi'. a gastrocamera examination undertaken as a result of her clinical history of gastric anisakiasis indicated that a large number of worms had penetrated the posterior walls. fifty-six larval nematodes were directly removed from the greater curvature of the stomach with a gastroendoscopic biopsy clipper, and were identified as the larvae of anisakis simpl ...	1992	1587691
[morphological and taxonomical studies on anisakidae larvae found in marine fishes of china. ii. gulf of tong king].	a survey on anisakidae larvae in 29 species (134 specimens) of marine fishes in the gulf of tong king has been carried out. anisakidae larvae were detected in 15 out of 29 species. the detected specimens were identified as larvae of anisakis simplex, hysterothylacium and pseudoterranova. the parasitization rate of anisakis simplex larvae, the main pathogen of anisakiasis, in fishes was 30.6% (41/134), while the parasitization rates of hysterothylacium and pseudoterranova larvae were comparativel ...	1992	1394905
radiolabelling of the excretory-secretory and somatic antigens of anisakis simplex larvae.	anisakis simplex larvae were cultured in vitro in medium containing 35s-methionine for ten days. the medium and the larval tissues were analysed for biosynthetically labelled polypeptide by sodium dodecyl sulphate polyacrylamide gel electrophoresis and autoradiography. immunoprecipitates with positive and negative human antisera were similarly analysed, using staphylococcus aureus to absorb immuno-complexes. es products of anisakis larvae contained many polypeptides with molecular weights of les ...	1992	1293198
molecular cloning of the cdna encoding a 42 kda antigenic polypeptide of anisakis simplex larvae.	the gene encoding an antigenic polypeptide of anisakis simplex larvae was studied using recombinant dna techniques. cdna synthesized from poly(a)-rich mrna from a. simplex larvae was ligated into phage vector lambda gtll dna and packaged in vitro. the phages were propagated on escherichia coli and a lambda gtll expression library was constructed. a cdna clone encoding a 42 kda antigenic polypeptide was selected by immunoscreening of the library and identified by the epitope selection method. a c ...	1992	1281853
[pseudotumor form of herring worm disease].		1975	1220077
anisakis simplex and anisakis physeteris: physicochemical properties of larval and adult hemoglobins.		1979	573208
response of mink (mustela vison) to larval anisakis simplex (nematoda: ascaridida).		1977	562861
[the herring worm disease (anisakiasis)].		1979	452757