Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| development of gendine-coated cannula for continuous subcutaneous insulin infusion for extended use. | continuous subcutaneous insulin infusion (csii) using pumps is a widely used method for insulin therapy in patients with diabetes mellitus. among the major factors that usually lead to the discontinuation of csii are csii set-related issues, including infection at the infusion site. the american diabetic association currently recommends rotating sites every 2 to 3 days. this recommendation adds cost and creates inconvenience. therefore, in order to prevent infections and extend the duration betw ... | 2015 | 25941227 |
| induction of ros generation by fluconazole in candida glabrata: activation of antioxidant enzymes and oxidative dna damage. | in this study, we assessed the generation of reactive oxygen species (ros) induced by subinhibitory concentration of fluconazole in susceptible and resistant candida glabrata strains at stationary growth phase and measured their oxidative responses parameters: glutathione peroxidase (gpx), superoxide dismutase (sod), glutathione-s-transferase (gst), consumption of hydrogen peroxide, and total glutathione, as well as oxidative damage in lipids, proteins, and dna. data showed that fluconazole incr ... | 2015 | 25935629 |
| antifungal activity of ellagic acid in vitro and in vivo. | ellagic acid (ea) has been shown to have antioxidant, antibacterial, and anti-inflammatory activities. in uighur traditional medicine, euphorbia humifusa willd is used to treat fungal diseases, and recent studies suggest that it is the ea content which is responsible for its therapeutic effect. however, the effects of ea on antifungal activity have not yet been reported. this study aimed to investigate the inhibitory effect of ea on fungal strains both in vitro and in vivo. the minimal inhibitor ... | 2015 | 25919446 |
| urinary tract infections and candida albicans. | urinary tract candidiasis is known as the most frequent nosocomial fungal infection worldwide. candida albicans is the most common cause of nosocomial fungal urinary tract infections; however, a rapid change in the distribution of candida species is undergoing. simultaneously, the increase of urinary tract candidiasis has led to the appearance of antifungal resistant candida species. in this review, we have an in depth look into candida albicans uropathogenesis and distribution of the three most ... | 2015 | 25914847 |
| candida survival strategies. | only few candida species, e.g., candida albicans, candida glabrata, candida dubliniensis, and candida parapsilosis, are successful colonizers of a human host. under certain circumstances these species can cause infections ranging from superficial to life-threatening disseminated candidiasis. the success of c. albicans, the most prevalent and best studied candida species, as both commensal and human pathogen depends on its genetic, biochemical, and morphological flexibility which facilitates adap ... | 2015 | 25911234 |
| phytase-producing capacity of yeasts isolated from traditional african fermented food products and phypk gene expression of pichia kudriavzevii strains. | phytate is known as a strong chelate of minerals causing their reduced uptake by the human intestine. ninety-three yeast isolates from traditional african fermented food products, belonging to nine species (pichia kudriavzevii, saccharomyces cerevisiae, clavispora lusitaniae, kluyveromyces marxianus, millerozyma farinosa, candida glabrata, wickerhamomyces anomalus, hanseniaspora guilliermondii and debaryomyces nepalensis) were screened for phytase production on solid and liquid media. 95% were a ... | 2015 | 25910031 |
| candida glabrata fungaemia at an australian cancer centre: epidemiology, risk factors and therapy. | 2015 | 25907425 | |
| candida biomarkers in patients with candidaemia and bacteraemia. | microbiological strategies are necessary to help clinicians discontinue empirical antifungal therapy in patients with suspected invasive candidiasis. culture methods and biomarkers each show low sensitivity. we analysed the value of combining different biomarkers as a decision-making tool for discontinuing empirical antifungal treatment. | 2015 | 25900160 |
| antifungal susceptibilities of candida, cryptococcus neoformans and aspergillus fumigatus from the asia and western pacific region: data from the sentry antifungal surveillance program (2010-2012). | the sentry antifungal surveillance program monitors global susceptibility rates of newer and established antifungal agents. we report the in vitro activity of seven antifungal agents against 496 contemporary clinical isolates of yeasts and molds. the isolates were obtained from 20 laboratories in the asia-western pacific (apac) region during 2010 through 2012. anidulafungin, caspofungin, micafungin, fluconazole, itraconazole, posaconazole and voriconazole were susceptibility tested using clsi me ... | 2015 | 25899126 |
| antifungal susceptibility profiles of bloodstream yeast isolates by sensititre yeastone over nine years at a large italian teaching hospital. | sensititre yeastone (syo) is an affordable alternative to the clinical and laboratory standards institute (clsi) reference method for antifungal susceptibility testing. in this study, the mics of yeast isolates from 1,214 bloodstream infection episodes, generated by syo during hospital laboratory activity (january 2005 to december 2013), were reanalyzed using current clsi clinical breakpoints/epidemiological cutoff values to assign susceptibility (or the wild-type [wt] phenotype) to systemic ant ... | 2015 | 25896705 |
| epidemiology of oropharyngeal candidiasis in human immunodeficiency virus/acquired immune deficiency syndrome patients and cd4+ counts. | the present study was directed to evaluate the forms of oropharyngeal candidiasis (opc) and their correlation with cd4+ cell counts in human immunodeficiency virus (hiv) patients. | 2015 | 25878473 |
| the ever-evolving landscape of candidaemia in patients with acute leukaemia: non-susceptibility to caspofungin and multidrug resistance are associated with increased mortality. | the epidemiology and clinical course of candidaemia in patients with acute leukaemia, a population frequently exposed to antifungals, have not been extensively studied. in the present contemporary series of acute leukaemia patients, we describe patient characteristics, candida species and mic distributions and investigate the association between antifungal resistance and all-cause mortality. | 2015 | 25855759 |
| neutrophil activation by candida glabrata but not candida albicans promotes fungal uptake by monocytes. | candida albicans and candida glabrata account for the majority of candidiasis cases worldwide. although both species are in the same genus, they differ in key virulence attributes. within this work, live cell imaging was used to examine the dynamics of neutrophil activation after confrontation with either c. albicans or c. glabrata. analyses revealed higher phagocytosis rates of c. albicans than c. glabrata that resulted in stronger pmn (polymorphonuclear cells) activation by c. albicans. furthe ... | 2015 | 25850517 |
| in vitro and in vivo antimicrobial evaluation of alkaloidal extracts of enantia chlorantha stem bark and their formulated ointments. | the in vitro and in vivo antimicrobial evaluation of the formulated ointment of alkaloidal extract of enantia chlorantha oliv. (annonaceae) was the concern of this study. the alkaloidal fraction of the stem bark extract was formulated into simple ointment using british pharmacopoeia formula for preparation of simple ointment. agar diffusion and agar dilution methods were used for the in vitro antimicrobial studies. ketoconazole 4000 μg/ml and tioconazole cream 1% were used as reference standards ... | 2015 | 25850210 |
| prevalence of albicans and non-albicans candiduria in a malaysian medical centre. | to determine the proportion of albicans and non-albicans candiduria in a hospital setting and to ascertain if fluconazole is still suitable as empirical antifungal therapy based on antifungal susceptibility patterns of candida species. | 2014 | 25842581 |
| maldi-tof typing highlights geographical and fluconazole resistance clusters in candida glabrata. | utilizing matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectra for candida glabrata typing would be a cost-effective and easy-to-use alternative to classical dna-based typing methods. this study aimed to use maldi-tof for the typing of c. glabrata clinical isolates from various geographical origins and test its capacity to differentiate between fluconazole-sensitive and -resistant strains.both microsatellite length polymorphism (mlp) and maldi-tof mass spectra of 5 ... | 2015 | 25841053 |
| evaluation of microbial load in oropharyngeal mucosa from tannery workers. | animal skin provides an ideal medium for the propagation of microorganisms and it is used like raw material in the tannery and footware industry. the aim of this study was to evaluate and identify the microbial load in oropharyngeal mucosa of tannery employees. | 2014 | 25830072 |
| sphingolipids mediate differential echinocandin susceptibility in candida albicans and aspergillus nidulans. | the cell wall synthesis-inhibiting echinocandins, including caspofungin and micafungin, play important roles in the treatment of candidiasis and aspergillosis. previous studies revealed that, in the haploid yeast candida glabrata, sphingolipid biosynthesis pathway mutations confer caspofungin reduced susceptibility (crs) but micafungin increased susceptibility (mis). here, we describe one candida albicans strain (of 10 tested) that similarly yields crs-mis mutants at relatively high frequency. m ... | 2015 | 25824222 |
| declining incidence of candidemia and the shifting epidemiology of candida resistance in two us metropolitan areas, 2008-2013: results from population-based surveillance. | recent reports have demonstrated a decline in bacterial bloodstream infections (bsis) following adherence to central line insertion practices; however, declines have been less evident for bsis due to candida species. | 2015 | 25822249 |
| early-onset candida glabrata interface keratitis after deep anterior lamellar keratoplasty. | fungal interface keratitis by candida species can occur several weeks to several months after deep anterior lamellar keratoplasty (dalk). here, we report a case of early-onset fungal interface keratitis by candida glabrata after dalk. | 2015 | 25822017 |
| mechanisms of azole resistance among clinical isolates of candida glabrata in poland. | candida glabrata is currently ranked as the second most frequently isolated aetiological agent of human fungal infections, next only to candida albicans. in comparison with c. albicans, c. glabrata shows lower susceptibility to azoles, the most common agents used in treatment of fungal infections. interestingly, the mechanisms of resistance to azole agents in c. albicans have been much better investigated than those in c. glabrata. the aim of the presented study was to determine the mechanisms o ... | 2015 | 25818698 |
| candida glabrata candidemia: an emerging threat in critically ill patients. | candidemia is an important nosocomial blood stream infection in critically ill patients. although several studies have addressed candidemia, very few have reviewed the impact of candida glabrata candidemia in intensive care unit (icu) patients. | 2015 | 25810610 |
| candida glabrata candidemia. | 2015 | 25810607 | |
| essential oils, silver nanoparticles and propolis as alternative agents against fluconazole resistant candida albicans, candida glabrata and candida krusei clinical isolates. | development of effective and safe therapeutic treatment of fungal infections remains one of the major challenge for modern medicine. the aim of presented investigation was to analyze the in vitro antifungal activity of selected essential oils, ethanolic extracts of propolis and silver nanoparticles dropped on tio2 against azole-resistant c. albicans (n = 20), c. glabrata (n = 14) and c. krusei (n = 10) clinical isolates. among tested essential oils, the highest activity has definitely been found ... | 2015 | 25805904 |
| in vitro fungicidal activities of anidulafungin, caspofungin, and micafungin against candida glabrata, candida bracarensis, and candida nivariensis evaluated by time-kill studies. | anidulafungin, caspofungin, and micafungin killing activities against candida glabrata, candida bracarensis, and candida nivariensis were evaluated by the time-kill methodology. the concentrations assayed were 0.06, 0.125, and 0.5 μg/ml, which are achieved in serum. anidulafungin and micafungin required between 13 and 26 h to reach the fungicidal endpoint (99.9% killing) against c. glabrata and c. bracarensis. all echinocandins were less active against c. nivariensis. | 2015 | 25801575 |
| significantly improved thermostability of a reductase cgkr1 from candida glabrata with a key mutation at asp 138 for enhancing bioreduction of aromatic α-keto esters. | the keto ester reductase from candida glabrata, designated as cgkr1, is a highly versatile biocatalyst with a broad substrate spectrum. its substrate preference was altered by rational design of the active pocket for bioreduction of aromatic α-keto esters in our previous work. however, its practical application is still hindered by its poor thermostability and high biocatalyst loading. in this work, random mutagenesis followed by a saturation mutagenesis was performed aiming to improve its therm ... | 2015 | 25795440 |
| of mice, flies--and men? comparing fungal infection models for large-scale screening efforts. | studying infectious diseases requires suitable hosts for experimental in vivo infections. recent years have seen the advent of many alternatives to murine infection models. however, the use of non-mammalian models is still controversial because it is often unclear how well findings from these systems predict virulence potential in humans or other mammals. here, we compare the commonly used models, fruit fly and mouse (representing invertebrate and mammalian hosts), for their similarities and deg ... | 2015 | 25786415 |
| the mitogen-activated protein kinase cghog1 is required for iron homeostasis, adherence and virulence in candida glabrata. | candida glabrata has emerged as a major fungal pathogen over the last two decades, although our understanding of its survival strategies inside the mammalian host remains rudimentary. an important requirement for survival in vivo is the ability to acquire critical nutrients such as iron from host niches of varied iron content. in the present study, we demonstrate for the first time that c. glabrata cells respond to high external iron levels via activation of two stress-responsive mitogen-activat ... | 2015 | 25772226 |
| epidemiology, antifungal susceptibilities, and risk factors for invasive candidiasis from 2011 to 2013 in a teaching hospital in southwest china. | invasive candidiasis (ic) is the most common cause of invasive fungal infections. identification of risk factors for such infection may help in the empirical therapeutic decision-making process. we conducted this study to characterize the clinical epidemiology of such infection and to differentiate risk factors between candida albicans and candida non-albicans species. | 2017 | 25769664 |
| effects of bovine lactoferrin to oral candida albicans and candida glabrata isolates recovered from the saliva in elderly people. | the effects of bovine lactoferrin (blf) on the growth of candida species and on inflammatory cytokine production in gingival keratinocytes, ndusd-1 co-cultured with candida strains were investigated. the results showed that blf at 10 and 100 lg/ml significantly inhibits the growth of two c. albicans strains and two c. glabrata strains isolated from the saliva of elderly people requiring nursing care, respectively. the levels of inflammatory cytokines, interleukin (il)-6, and il-8 in ndusd-1 cocu ... | 2015 | 25756168 |
| structural and mechanistic analysis of the slx1-slx4 endonuclease. | the slx1-slx4 endonuclease required for homologous recombination and dna repair in eukaryotic cells cleaves a variety of branched dna structures. the nuclease subunit slx1 is activated by association with a scaffolding protein slx4. at the present time, little is known about the structure of slx1-slx4 or its mechanism of action. here, we report the structural insights into slx1-slx4 by detailing the crystal structure of candida glabrata (cg) slx1 alone and in combination with the c-terminal regi ... | 2015 | 25753413 |
| avoiding the ends: internal epitope tagging of proteins using transposon tn7. | peptide tags fused to proteins are used in a variety of applications, including as affinity tags for purification, epitope tags for immunodetection, or fluorescent protein tags for visualization. however, the peptide tags can disrupt the target protein function. when function is disrupted by fusing a peptide to either the n or c terminus of the protein of interest, identifying alternative ways to create functional tagged fusion proteins can be difficult. here, we describe a method to introduce p ... | 2015 | 25745023 |
| yap7 is a transcriptional repressor of nitric oxide oxidase in yeasts, which arose from neofunctionalization after whole genome duplication. | flavohemoglobins are the main detoxifiers of nitric oxide (no) in bacteria and fungi and are induced in response to nitrosative stress. in fungi, the flavohemoglobin encoding gene yhb1 is positively regulated by transcription factors which are activated upon no exposure. in this study, we show that in the model yeast saccharomyces cerevisiae and in the human pathogen candida glabrata, the transcription factor yap7 constitutively represses yhb1 by binding its promoter. consequently, yap7 deletion ... | 2015 | 25732006 |
| [fungal infections and ureteral material: how to manage?]. | urinary tract infections due to candida species are mostly encountered in hospital environment. the management of candiduria on ureteral catheter is not consensual. the objective of our work was to make a review of medical literature related to definition, physiopathology, management and prevention of candiduria on ureteral catheter. | 2015 | 25724861 |
| design, synthesis and in vitro bactericidal/fungicidal screening of some vanadyl(iv)complexes with mono- and di-substituted ons donor triazoles. | a new series of anti-bacterial and anti-fungal mono- and di-substituted triazoles (l(1))-(l(6)) have been synthesized and characterized on the basis of their physical, spectral and analytical data. the ligands (l(1))-(l(6)) on reaction with vanadyl(iv) sulphate led to the formation of vanadyl(iv) metal complexes (1)-(4). the structure of the complexes has been established on the basis of their physical, spectral and elemental analyses data. the synthesized ligands and their vanadyl(iv) complexes ... | 2015 | 25716124 |
| guideline: vulvovaginal candidosis (awmf 015/072), s2k (excluding chronic mucocutaneous candidosis). | the oestrogenised vagina is colonised by candida species in at least 20% of women; in late pregnancy and in immunosuppressed patients, this increases to at least 30%. in most cases, candida albicans is involved. host factors, particularly local defence mechanisms, gene polymorphisms, allergies, serum glucose levels, antibiotics, psycho-social stress and oestrogens influence the risk of candidal vulvovaginitis. non-albicans species, particularly candida glabrata, and in rare cases also saccharomy ... | 2015 | 25711406 |
| anti-candida activity of quercus infectoria gall extracts against candida species. | galls of quercus infectoria have been traditionally used to treat common ailments, including yeast infections caused by candida species. | 2017 | 25709331 |
| mitochondrial engineering of the tca cycle for fumarate production. | microbial fumarate production from renewable feedstock is a promising and sustainable alternative to petroleum-based chemical synthesis. here, mitochondrial engineering was used to construct the oxidative pathway for fumarate production starting from the tca cycle intermediate α-ketoglutarate in candida glabrata. accordingly, α-ketoglutarate dehydrogenase complex (kgd), succinyl-coa synthetase (suclg), and succinate dehydrogenase (sdh) were selected to be manipulated for strengthening the oxidat ... | 2015 | 25708514 |
| inherited card9 deficiency in otherwise healthy children and adults with candida species-induced meningoencephalitis, colitis, or both. | invasive infections of the central nervous system (cns) or digestive tract caused by commensal fungi of the genus candida are rare and life-threatening. the known risk factors include acquired and inherited immunodeficiencies, with patients often displaying a history of multiple infections. cases of meningoencephalitis, colitis, or both caused by candida species remain unexplained. | 2015 | 25702837 |
| terminal-stage prognostic analysis in candidemia. | candidemia has an extremely high mortality rate but is not always the direct cause of death. therefore, determining the effect of candidemia on death is extremely difficult. | 2015 | 25697387 |
| probiotic lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing candida glabrata isolates. | this study investigates the antagonistic effects of the probiotic strains lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 against vulvovaginal candidiasis (vvc)-causing candida glabrata. | 2015 | 25688886 |
| synthesis, qsar and anticandidal evaluation of 1,2,3-triazoles derived from naturally bioactive scaffolds. | in the present study, we used eight natural precursors (1a-h) with most of them having promising antimicrobial activities and synthesised their novel 1,2,3-triazole derivatives (3a-h). in the reaction sequences, the precursor compounds (1a-h) were converted to their respective alkyne (2a-h) followed by addition of benzyl azide freshly prepared by the reaction of benzyl bromide with sodium azide using [3 + 2] azide-alkyne cycloaddition strategy. structural elucidation of all the triazole derivati ... | 2015 | 25686593 |
| [evaluation of the rapid trehalose test for the identification of the candida glabrata]. | to explore a rapid and cost-effective method for identification of candida glabrata through the comparison of two different methods, using molecular methods of sequencing as gold standard. | 2015 | 25686354 |
| the effect of antifungal combination on transcripts of a subset of drug-resistance genes in clinical isolates of candida species induced biofilms. | biofilm formation is often associated with increased candida resistance toward antifungal agents. therefore, the current study aimed to assess the incidence of biofilm formation among candida isolates and to investigate the effect of high doses of fluconazole {flc}, voriconazole {voc} and amphotericin b {amb}, singly and in combination on mature biofilms. moreover, it aimed to assess the expression of selected genes (cdr1, kre1 and skn1) responsible for candida biofilm resistance. the study incl ... | 2015 | 25685044 |
| digestive tract mycobiota: a source of infection. | the human mycobiome includes 390 fungal species detected on the skin, in the vagina, in the oral cavity, and in the digestive tract that includes 335 species and 158 genera. among these, 221 species are found only in the digestive tract, 88 only in the oral cavity, and 26 in both. these species belong to 126 genera of yeast and filamentous fungi, of the ascomycota, basidiomycota, and zygomycota phyla. forty species were identified only by culture, 188 species by molecular techniques, and 19 spec ... | 2015 | 25684583 |
| patterns of candida biofilm on intrauterine devices. | biofilms are colonies of microbial cells encased in a self-produced organic polymeric matrix and represent a common mode of microbial growth. microbes growing as biofilm are highly resistant to commonly used antimicrobial drugs. we aimed to screen and characterize biofilm formation by different isolates of candida on removed intrauterine devices (iuds), to perform experimental biofilm formation with isolated strains, and to examine biofilm by the crystal violet and xtt reduction assays and scann ... | 2015 | 25681320 |
| site-specific mesenchymal control of inflammatory pain to yeast challenge in vulvodynia-afflicted and pain-free women. | fibroblast strains were derived from 2 regions of the lower genital tract of localized provoked vulvodynia (lpv) cases and pain-free controls. sixteen strains were derived from 4 cases and 4 controls, age and race matched, after presampling mechanical pain threshold assessments. strains were challenged with 6 separate stimuli: live yeast species (candida albicans, candida glabrata, candida tropicalis, and saccharomyces cerevisiae), yeast extract (zymosan), or inactive vehicle. production of pros ... | 2015 | 25679469 |
| design of micelle nanocontainers based on pdmaema-b-pcl-b-pdmaema triblock copolymers for the encapsulation of amphotericin b. | the clinical application of amphotericin b (amb), a broad spectrum antifungal agent, is limited by its poor solubility in aqueous medium and also by its proven renal toxicity. in this work, amb was encapsulated in micelles obtained from the self-assembly of pdmaema-b-pcl-b-pdmaema triblock copolymers. the amount of encapsulated amb depended on the copolymer composition, and short blocks of polycaprolactone (pcl) and poly(2-dimethylaminoethyl methacrylate) (pdmaema) showed better performance. all ... | 2015 | 25669917 |
| current methods for identifying clinically important cryptic candida species. | in recent years, the taxonomy of the most important pathogenic candida species (candida albicans, candida parapsilosis and candida glabrata) has undergone profound changes due to the description of new closely-related species. this has resulted in the establishment of cryptic species complexes difficult to recognize in clinical diagnostic laboratories. the identification of these novel candida species seems to be clinically relevant because it is likely that they differ in virulence and drug res ... | 2015 | 25659326 |
| susceptibility characterisation of candida spp. to four essential oils. | in the present investigation, anti-candida activity of four essential oils i.e. black cumin (nigella sativa), curry leaf (murraya koienigii), ajwain (trachiyspirum ammi), and betel leaf (piper betel) were screened against four human pathogenic species of candida viz. candida albicans, candida tropicalis, candida glabrata, and candida parapsilosis. the minimum inhibitory concentration (mic) values of the oils ranged between 15.62 and 250 μl/ml while studied through tube dilution method. the oils ... | 2015 | 25657164 |
| invasive candida infections in surgical patients in intensive care units: a prospective, multicentre survey initiated by the european confederation of medical mycology (ecmm) (2006-2008). | a prospective, observational, multicentre study of invasive candidosis (ic) in surgical patients in intensive care units (icus) was conducted from 2006 to 2008 in 72 icus in 14 european countries. a total of 779 patients (62.5% males, median age 63 years) with ic were included. the median rate of candidaemia was 9 per 1000 admissions. in 10.8% the infection was already present at the time of admission to icu. candida albicans accounted for 54% of the isolates, followed by candida parapsilosis 18 ... | 2015 | 25636940 |
| early initiation of appropriate treatment is associated with increased survival in cancer patients with candida glabrata fungaemia: a potential benefit from infectious disease consultation. | in patients with malignancies, candida glabrata is one of the most frequent non-albicans candida clinical isolates. as antifungal resistance in c. glabrata is common, we investigated the relationship between early appropriate antifungal treatment, infectious disease (id) consultation and mortality in a contemporary cohort of cancer patients with c. glabrata fungaemia. we included patients with at least one c. glabrata-positive blood culture and symptoms or signs of infection seen at the md ander ... | 2015 | 25636931 |
| force nanoscopy of hydrophobic interactions in the fungal pathogen candida glabrata. | candida glabrata is an opportunistic human fungal pathogen which binds to surfaces mainly through the epa family of cell adhesion proteins. while some epa proteins mediate specific lectin-like interactions with human epithelial cells, others promote adhesion and biofilm formation on plastic surfaces via nonspecific interactions that are not yet elucidated. we report the measurement of hydrophobic forces engaged in epa6-mediated cell adhesion by means of atomic force microscopy (afm). using singl ... | 2015 | 25621738 |
| determination of micing: a new assay for assessing minimal inhibitory concentration for invasive growth. | our work was focused on a new assay for characterising clinically important yeast. this assay was developed due to the need for new diagnostic methods for recognising potentially virulent strains of increasingly important non-albicans yeast pathogens, such as saccharomyces cerevisiae and candida glabrata. with the great diversity among strains for virulence and virulence factors, identification to the species level is not sufficient; therefore, testing for specific virulent traits remains the be ... | 2015 | 25620781 |
| a novel candida glabrata cell wall associated serine protease. | we set out to identify the candida glabrata cell wall attached proteases which may play a role as virulence factors in candidosis, particularly in the immunocompromized host. we studied a clinical c. glabrata strain t-1639, which was isolated from a patient from the helsinki university central hospital. with non-reducing 2-d electrophoresis using parallel fluorogenic gels and mass spectrometry we identified a novel appr. 25 kda (192 aa in length) cell wall located protease with an estimated pi o ... | 2015 | 25617734 |
| detection and quantification of fluconazole within candida glabrata biofilms. | candida infections are often associated with biofilms and consequent high resistance to most common drugs (e.g. azoles). these resistance mechanisms are not only associated with the biofilm yeast physiology, but also with the presence of a diffusional barrier imposed by the biofilm matrix; however, the real biochemical role of the biofilm components remains very unclear. so, in order to further clarify this issue, we intend to determine, for the first time, fluconazole in biofilms within both su ... | 2015 | 25614085 |
| candida glabrata and candida tropicalis in an immunocompetent patient: a case report. | to report a case of candida glabrata and tropicalis pneumonia in an immunocompetent patient. | 2015 | 25613052 |
| persistence of nosocomial pathogens on various fabrics. | fabrics can become contaminated with high numbers of microorganisms that may be pathogenic to patients in a hospital setting and can play an important role in the chain of infection. the aim of this study was to investigate the survival of several clinical bacterial and fungal isolates on several fabrics commonly used in hospitals. | 2012 | 25610201 |
| candida glabrata perinephric abscess. | perinephric abscess is a rare complication of urinary tract infections, and the etiology is usually a gram negative bacillus. we report a case of candida glabrata perinephric abscess in a patient with diabetes mellitus who had a recent episode of pyelonephritis that was treated with antibiotics. percutaneous drainage and fluconazole treatment led to resolution of the infection. yeast perinephric abscess should be considered when symptoms of acute or chronic urinary tract infection occur in elder ... | 2011 | 25610164 |
| investigating biofilm production, coagulase and hemolytic activity in candida species isolated from denture stomatitis patients. | oral candidiasis, in the form of candida-associated denture stomatitis, represents a common disease in a large percentage of denture wearers, and candida albicans remains the most commonly isolated species. in this study, we aimed to evaluate biofilm production, coagulase and hemolytic activity of candida species isolated from denture stomatitis patients. | 2011 | 25610156 |
| in vitro activity of 23 tea extractions and epigallocatechin gallate against candida species. | in this study, we investigate the susceptibility of candida albicans, candida glabrata, candida krusei, candida parapsilosis, candida tropicalis, and aspergillus fumigatus using the eucast microdilution minimum inhibitory concentration (mic) method (final tea supernatant concentration range 5.0-0.005 mg/ml) to 23 different teas and tea catechins including epigallocatechin gallate (egcg) isolated from green tea. all teas exhibited potent in vitro antifungal activity against c. glabrata. six out o ... | 2015 | 25605775 |
| analysis of the essentiality of rom2 genes in the pathogenic yeasts candida glabrata and candida albicans using temperature-sensitive mutants. | to analyse the essentiality of the rom2 genes originating from the pathogenic yeasts candida glabrata and candida albicans by using temperature-sensitive (ts) mutants. | 2015 | 25604069 |
| candida albicans opi1 regulates filamentous growth and virulence in vaginal infections, but not inositol biosynthesis. | scopi1p is a well-characterized transcriptional repressor and master regulator of inositol and phospholipid biosynthetic genes in the baker's yeast saccharomyces cerevisiae. an ortholog has been shown to perform a similar function in the pathogenic fungus candida glabrata, but with the distinction that cgopi1p is essential for growth in this organism. however, in the more distantly related yeast yarrowia lipolytica, the opi1 homolog was not found to regulate inositol biosynthesis, but alkane oxi ... | 2015 | 25602740 |
| in vitro activity of a hos2 deacetylase inhibitor, mgcd290, in combination with echinocandins against echinocandin-resistant candida species. | mgcd290, a hos2 fungal histone deacetylase inhibitor, showed modest activity when tested alone (mic range, 0.12-4 μg/ml; mic50/90, 0.5/4 μg/ml) against candida glabrata (n=15; 14 fks mutants; 5 also fluconazole resistant), candida albicans (8 fks mutants; 2 also fluconazole resistant), candida tropicalis (4 fks mutants), and candida krusei (3 fks mutants). however, mgcd290 showed synergy or partial synergy for 33.3%, 30.1%, 36.7%, and 80.0% of the isolates when tested with anidulafungin, caspofu ... | 2015 | 25600842 |
| molecular identification and antifungal susceptibility of 186 candida isolates from vulvovaginal candidiasis in southern china. | there is limited information regarding the molecular epidemiology and antifungal susceptibilities of candida isolates using the neo-sensitabs method in patients with vulvovaginal candidiasis (vvc). from august 2012 to march 2013, 301 non-pregnant patients aged 18-50 years with suspected vvc were prospectively screened at a teaching hospital in southern china. the vaginal isolates were identified by dna sequencing of internal transcribed spacer and the d1/d2 domain. antifungal susceptibility test ... | 2015 | 25596116 |
| t2 magnetic resonance assay for the rapid diagnosis of candidemia in whole blood: a clinical trial. | microbiologic cultures, the current gold standard diagnostic method for invasive candida infections, have low specificity and take up to 2-5 days to grow. we present the results of the first extensive multicenter clinical trial of a new nanodiagnostic approach, t2 magnetic resonance (t2mr), for diagnosis of candidemia. | 2015 | 25586686 |
| the epa2 adhesin encoding gene is responsive to oxidative stress in the opportunistic fungal pathogen candida glabrata. | candida glabrata has emerged as an important opportunistic pathogen in both mucosal and bloodstream infections. c. glabrata contains 67 adhesin-like glycosylphosphatidylinositol-cell-wall proteins (gpi-cwps), which are classified into seven groups and the largest is the epa family. epa proteins are very diverse and their expression is differentially regulated. like many of the epa genes, epa2 is localized in a subtelomeric region where it is subject to chromatin-based transcriptional silencing a ... | 2015 | 25586543 |
| defining the transcriptomic landscape of candida glabrata by rna-seq. | candida glabrata is the second most common pathogenic candida species and has emerged as a leading cause of nosocomial fungal infections. its reduced susceptibility to antifungal drugs and its close relationship to saccharomyces cerevisiae make it an interesting research focus. although its genome sequence was published in 2004, little is known about its transcriptional dynamics. here, we provide a detailed rna-seq-based analysis of the transcriptomic landscape of c. glabrata in nutrient-rich me ... | 2015 | 25586221 |
| in vitro evaluation of the acquisition of resistance, antifungal activity and synergism of brazilian red propolis with antifungal drugs on candida spp. | to evaluate the ability of candida parapsilosis and candida glabrata to develop phenotypic resistance to a benzophenone enriched fraction obtained from brazilian red propolis (bzp-brp) as compared to fluconazole (flc). to investigate possible synergy between bzp-brp and flc and anidulafungin (and). | 2015 | 25565139 |
| chemical composition and antimicrobial evaluation of the essential oils of bocageopsis pleiosperma maas. | essential oils from the leaves, twigs and barks of bocageopsis pleiosperma maas were obtained by using hydrodistillation and analysed by using gas chromatography coupled to mass spectrometry. several compounds (51) were detected and identified, being β-bisabolene the main component in all aerial parts of the plant, with higher concentration in the leaves (55.77%), followed by barks (38.53%) and twigs (34.37%). in order to increase the biological knowledge about the essential oil of bocageopsis s ... | 2015 | 25562370 |
| emergence of multiple resistance profiles involving azoles, echinocandins and amphotericin b in candida glabrata isolates from a neutropenia patient with prolonged fungaemia. | 2015 | 25550394 | |
| candida glabrata esophagitis: are we seeing the emergence of a new azole-resistant pathogen? | background. candida glabrata (c. glabrata) has become a recognized pathogen in fungal esophagitis. a proportion of these isolates are azole-resistant which may have treatment implications. variability in the prevalence of this organism exists in the limited data available. objective. to determine the incidence of c. glabrata esophagitis in a north american hospital setting and to highlight factors that may predispose patients to this condition. methods. patient charts were collected from january ... | 2014 | 25544843 |
| [yeast species in vulvovaginitis candidosa]. | vulvovaginal candidiasis is the most common mycosis, however, the available information about antifungal susceptibilities of these yeasts is limited. | 2015 | 25544052 |
| [oral candida species distribution in patients receiving radiotherapy for head and neck cancer]. | to investigate the distribution of oral candida species in head and neck cancer (hnc) patients treated with radiation therapy, and provide the basis for planning of clinical preventive measures. | 2014 | 25543608 |
| maldi-tof mass spectrometry for rapid identification of clinical fungal isolates based on ribosomal protein biomarkers. | this study aimed to evaluate the identification of clinical fungal isolates (yeast and molds) by protein profiling using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof/ms). a total of 125 clinical fungal culture isolates (yeast and filamentous fungi) were collected. the test set included 88 yeast isolates (candida albicans, candida glabrata, candida guilliermondii, candida kefyr, candida krusei, candida parapsilosis, candida rugosa, candida tropicalis and ... | 2015 | 25541362 |
| surveillance of antifungal susceptibilities in clinical isolates of candida species at 36 hospitals in china from 2009 to 2013. | the purpose of this study was to determine the species distribution and to monitor the antifungal susceptibility profiles of clinical candida isolates collected in china from 2009 to 2013. | 2015 | 25541294 |
| species spectrum and antifungal susceptibility profile of vaginal isolates of candida in kuwait. | the study was undertaken to determine the prevalence of vulvovaginal candidiasis (vvc) among patients with vaginitis, frequency of different candida species, and their susceptibility profile. | 2015 | 25534676 |
| lab-scale preparations of candida albicans and dual candida albicans-candida glabrata biofilms on the surface of medical-grade polyvinyl chloride (pvc) perfusion tube using a modified gravity-supported free-flow biofilm incubator (gs-ffbi). | the assembly of a man-made gravity-supported free-flow biofilm incubator (gs-ffbi) was described, which was composed of a gas cushion injector and four incubators. the gs-ffbi had the characteristics of (i) a bottom-up flow direction, and (ii) lab-scale biofilm preparation without the use of a multichannel pump. two opportunistic fungal strains, namely candida albicans and candida glabrata, were employed to incubate c. albicans and dual c. albicans-c. glabrata biofilms on the surface of medical- ... | 2015 | 25528294 |
| contribution of (1,3)-beta-d-glucan to diagnosis of invasive candidiasis after liver transplantation. | invasive candidiasis (ic) causes high morbidity and mortality rates after liver transplantation, in part due to delayed diagnosis. the fungal cell wall component (1,3)-beta-d-glucan (bg) could be an early biomarker of ic. this preliminary prospective study was designed to evaluate the contribution of bg measurements to the diagnosis of ic after liver transplantation. all consecutive patients who underwent liver transplantation at henri mondor hospital in france between january and june 2013 were ... | 2015 | 25520448 |
| gastric trichobezoar associated with perforated peptic ulcer and candida glabrata infection. | bezoars are accumulations of human or plant fiber located in the gastrointestinal tract of both humans and animals. patients remain asymptomatic for several years, and the symptoms develop as these accumulations increase in size to the point of obstruction or perforation. we report the case of a 21-year-old patient at 10 d postpartum, who presented with acute abdomen associated with sepsis. given the urgency of the clinical picture, at no point was the presence of a giant bezoar at gastric level ... | 2014 | 25516871 |
| lupin peptone as a replacement for animal-derived peptone in rich culture media for yeast. | lupin peptone was shown to be a suitable replacement for traditional bacteriological peptone in the culture of candida glabrata, candida albicans and saccharomyces cerevisiae. this new medium formulation allows yeast researchers to increase safety and to eliminate the use of animal products for the culture of yeast in rich medium. | 2015 | 25514068 |
| candida glabrata sepsis associated with chorioamnionitis in an ivf twin pregnancy: should we deliver? | we report a case of in vitro fertilization (ivf)-acquired candida glabrata chorioamnionitis successfully treated through systemic maternal antifungal treatment prior to delivery. to the best of our knowledge, this is the first case of its kind in the literature. c. glabrata chorioamnionitis in pregnancy is rare, but the current literature suggests a very high fetal fatality in such cases. it is known to have an association with cervical stitch, amniocentesis, chorionic villous sampling, and assi ... | 2015 | 25510957 |
| [trend of susceptibility testing of clinical isolates of candida species from aseptic samples in hospitals in saitama prefecture]. | we investigated the susceptibility of candida species from clinical aseptic samples, including blood, at some hospitals in saitama prefecture. candida spp. detected from aseptic samples in the 6 institutes in saitama prefecture from november 2007 to july 2011 were studied. the number of isolates was 85, which are 43 (50.6%) of candida albicans, 24 (28.2%) of candida parapsilosis, 5 (5.9%) of candida glabrata, 5 (5.9%) of candida tropicalis, 4 (4.7%) of candida guilliermondii, 2 (2.4%) of candida ... | 2014 | 25507301 |
| the cdc13-stn1-ten1 complex stimulates pol α activity by promoting rna priming and primase-to-polymerase switch. | emerging evidence suggests that cdc13-stn1-ten1 (cst), an rpa-like ssdna-binding complex, may regulate primase-pol α (pp) activity at telomeres constitutively, and at other genomic locations under conditions of replication stress. here we examine the mechanisms of pp stimulation by cst using purified complexes derived from candida glabrata. while cst does not enhance isolated dna polymerase activity, it substantially augments both primase activity and primase-to-polymerase switching. cst also si ... | 2014 | 25503194 |
| in vitro anti-yeast activity of chloramphenicol: a preliminary report. | chloramphenicol is a bacteriostatic antimicrobial agent but its antifungal activity is not known. the present study aimed to investigate the activity of chloramphenicol against 30 representative yeasts. the antimicrobial assay of chloramphenicol (50mg/ml; 100mg/ml and 200mg/ml) was determined by the disc diffusion method using mueller-hinton agar against 30 representative yeast strains. zone of inhibition was read after 48-72h incubation at 37°c and results were compared with some standard antif ... | 2015 | 25497707 |
| pretreatment of chemically-synthesized aβ42 affects its biological activity in yeast. | the tendency of amyloid β (aβ42) peptide to misfold and aggregate into insoluble amyloid fibrils in alzheimer's disease (ad) has been well documented. accumulation of aβ42 fibrils has been correlated with abnormal apoptosis and unscheduled cell division which can also trigger the death of neuronal cells, while oligomers can also exhibit similar activities. while investigations using chemically-synthesized aβ42 peptide have become common practice, there appear to be differences in outcomes from d ... | 2014 | 25495906 |
| the superoxide dismutases of candida glabrata protect against oxidative damage and are required for lysine biosynthesis, dna integrity and chronological life survival. | the fungal pathogen candida glabrata has a well-defined oxidative stress response, is extremely resistant to oxidative stress and can survive inside phagocytic cells. in order to further our understanding of the oxidative stress response in c. glabrata, we characterized the superoxide dismutases (sods) cu,znsod (sod1) and mnsod (sod2). we found that sod1 is the major contributor to total sod activity and is present in cytoplasm, whereas sod2 is a mitochondrial protein. both sods played a central ... | 2015 | 25479837 |
| compartmentalizing metabolic pathway in candida glabrata for acetoin production. | acetoin, a valuable compound, has high potential as a biochemical building block. in this study, subcellular metabolic engineering was applied to engineer the mitochondrion of candida glabrata for acetoin production. with the aid of mitochondrial targeting sequences, a heterologous acetoin pathway was targeted into the mitochondria to increase the enzyme concentrations and level of intermediate, followed by coupling with the mitochondrial pyruvate carrier (mpc) to increase the availability of mi ... | 2015 | 25479455 |
| antifungal susceptibilities of candida glabrata species complex, candida krusei, candida parapsilosis species complex and candida tropicalis causing invasive candidiasis in china: 3 year national surveillance. | to define the antifungal susceptibility patterns of the most common non-albicans candida spp. in china. | 2015 | 25473027 |
| the mannoprotein tir3 (cagl0c03872g) is required for sterol uptake in candida glabrata. | sterol uptake in the pathogenic fungus, candida glabrata, occurs via the sterol transporter, cgaus1p. azole inhibition of sterol biosynthesis can under certain circumstances be reversed by adding exogenously sterol. here we demonstrate that the cgtir3 (cagl0c03872g) gene product is also required for sterol uptake, since cgtir3δ strains fail to take up sterol both aerobically and under hypoxic conditions. western analysis using an ha-tagged tir3 strain showed that cgtir3p localizes to the cell wa ... | 2015 | 25463012 |
| candida glabrata meningitis and endocarditis: a late severe complication of candidemia. | we report an unusual case of candida glabrata meningitis and endocarditis in a young caucasian woman with a prosthetic aortic valve and suffering from a dissecting thoraco-abdominal aortic aneurysm. c. glabrata was isolated from culture of the cerebrospinal fluid. candida infection of the central nervous system is an uncommon manifestation of disseminated infection due to candida species. our case report also highlights the intrinsic resistance of c. glabrata to azoles. | 2014 | 25449253 |
| [catheterization and fungal infection risk in the university hospital of tlemcen: epidemiology and susceptibility to antifungals]. | fungal infections are a major cause of morbidity and mortality, and are frequently associated with the implantation of vascular catheters, especially in immune-compromised patients. unfortunately, the therapeutic arsenal available for the treatment of these infections, caused generally by the yeasts of the genus candida is still limited because of the toxicity and/or of the emergence of resistance against some antifungal agents. that is why we have undertaken this study, which is to determine th ... | 2014 | 25442921 |
| comparative effects of hypoxia and hypoxia mimetic cobalt chloride on in vitro adhesion, biofilm formation and susceptibility to amphotericin b of candida glabrata. | candida glabrata has emerged as potent pathogen in nosocomial infections. the objective of this study is to investigate the effects of hypoxia (an important host factor) and hypoxia mimetic cobalt chloride upon growth, in vitro adhesion, biofilm formation and susceptibility to amphotericin b of candida glabrata. | 2014 | 25442919 |
| in vitro susceptibility profile of 200 recent clinical isolates of candida spp. to topical antifungal treatments of vulvovaginal candidiasis, the imidazoles and nystatin agents. | topical antifungal treatment of vulvovaginal candidiasis is widely recommended. the most commonly recommended topical antifungals (the imidazoles clotrimazole, miconazole and econazole and the polyene nystatin) have been on the market for more than 30 years. there are only a few recent data available on the susceptibility of different candida species to these antifungals, especially of non-albicans candida species which appear to be less responsive to treatment with imidazoles. the study aimed t ... | 2014 | 25442913 |
| failed reverse total shoulder arthroplasty caused by recurrent candida glabrata infection with prior serratia marcescens coinfection. | this report describes a 58-year-old insulin-dependent diabetic male patient who initially sustained a proximal humerus fracture from a fall. the fracture fixation failed and then was converted to a humeral hemiarthroplasty, which became infected with candida glabrata and serratia marcescens. after these infections were believed to be cured with antibacterial and antifungal treatments and two-stage irrigation and debridement, he underwent conversion to a reverse total shoulder arthroplasty. unfor ... | 2014 | 25431708 |
| genotypic, phenotypic, and proteomic characterization of candida glabrata during sequential fluconazole exposure. | candida glabrata is a major pathogen in humans known to be intrinsically resistant to fluconazole. however, genotypic, phenotypic, and proteomic changes associated with reduced susceptibility to fluconazole are not properly understood. the aim of this study was to observe specific phenotypic, chromosomal, and proteomic alterations in a candida glabrata strain sequentially exposed to fluconazole. | 2011 | 25426605 |
| [trend of susceptibility testing of clinical isolates of candida species from aseptic samples in hospitals in saitama prefecture]. | we investigated the susceptibility of candida species from clinical aseptic samples, including blood, at some hospitals in saitama prefecture. candida spp. detected from aseptic samples in the 6 institutes in saitama prefecture from november 2007 to july 2011 were studied. the number of isolates was 85, which are 43 (50.6%) of candida albicans, 24 (28.2%) of candida parapsilosis, 5 (5.9%) of candida glabrata, 5 (5.9%) of candida tropicalis, 4 (4.7%) of candida guilliermondii, 2 (2.4%) of candida ... | 2014 | 25420317 |
| management of persistent vaginitis. | with vaginitis remaining a common condition that leads women to seek care, it is not surprising that some women develop chronic vulvovaginal problems that are difficult to diagnose and treat. with a differential diagnosis that encompasses vulvar disorders and infectious and noninfectious causes of vaginitis, accurate diagnosis is the cornerstone of choosing effective therapy. evaluation should include a symptom-specific history, careful vulvar and vaginal examination, and office-based tests (vag ... | 2014 | 25415165 |
| in vivo candida glabrata biofilm development on foreign bodies in a rat subcutaneous model. | biofilm studies have been mostly dedicated to the major human fungal pathogen candida albicans, whereas much less is known about this virulence factor in candida glabrata, certainly under in vivo conditions. this study provides a deeper understanding of the biofilm development of c. glabrata, its architecture and susceptibility profile to fluconazole and echinocandins. | 2015 | 25406296 |
| use of candida-specific chicken egg yolk antibodies to inhibit the adhering of candida to denture base materials: prevention of denture stomatitis. | polyclonal anti-candida chicken egg yolk antibodies (anti-igy) were used to investigate the prevention of adherence of candida species to denture base material in vitro. | 2016 | 25393605 |