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disease development and symptom expression of xanthomonas axonopodis pv. citri in various citrus plant tissues.abstract experimental inoculations of xanthomonas axonopodis pv. citri in different tissues of tahiti lime and pineapple sweet orange were conducted monthly under natural conditions on réunion island. the interactions between a set of environmental and epidemic variables associated with disease expression and 184 different factor combinations were investigated to determine the parameters needed to explain asiatic citrus canker (acc) disease expression. area under the disease progress curve (audp ...200318943164
chimeras in the pineapple; colchicine-induced tetraploids and diploid-tetraploids in the cayenne variety. 194718902547
flower formation in the pineapple plant by geotropic stimulation. 194818875244
multi-walled carbon nanotubes as efficient solid-phase extraction materials of organophosphorus pesticides from apple, grape, orange and pineapple fruit juices.multi-walled carbon nanotubes (mwcnts) have been used for the first time as solid-phase extraction (spe) sorbents for the extraction of eight organophosphorus pesticides (i.e. ethoprophos, diazinon, chlorpyriphos-methyl, fenitrothion, malathion, chlorpyriphos, fenamiphos and buprofezin) from different commercial fruit juices (i.e. apple, grape, orange and pineapple). the developed method, which involves spe and direct gas chromatography with nitrogen phosphorus detection analysis, is very fast, ...200818849040
the effect of different hormones and incubation periods on in vitro proliferation of pineapple (ananas comosus l.) merr cv. smooth cayenne) shoot-tip culture.seven different hormone treatments, namely 6-benzylaminopurine (bap) at 2, 3 mg l(-1) was applied singly and in combination with indole acetic acid (iaa) at 0.18, 0.8 and 1.8 mg l(-l), bap at 3.3 mg l(-l) in combination with iaa at 1.8 and 3.3 mg l(-l) and triple combination of bap at 2.3, iaa at 1.8 and gibberellic acid (ga3) at 1.0 mg l(-1) were tested, over four different incubation periods of 30, 45, 60 and 75 days, for their effect in the proliferation and growth of smooth cayenne pineapple ...200818817160
preparation of ferulic acid from agricultural wastes: its improved extraction and purification.ferulic acid (fa) is a phenolic antioxidant present in plants, which is widely used in the food and cosmetic industry. in the present study, various agricultural wastes such as maize bran, rice bran, wheat bran, wheat straw, sugar cane baggasse, pineapple peels, orange peels, and pomegranate peels were screened for the presence of esterified fa (efa). among the sources screened, maize bran was found to contain the highest amount of efa. pineapple peels, orange peels, and pomegranate peels were a ...200818707110
trace metal levels in fruit juices and carbonated beverages in nigeria.trace metal levels in selected fruit juices and carbonated beverages purchased in lagos, nigeria were determined using atomic absorption spectrophotometer (unicam model 969) equipped with solaar 32 windows software. fruit juices analysed were grape, pineapple, apple, orange, lemon juices and their brand names were used. some carbonated drinks were also evaluated for metal levels. trace metals investigated were cr, cu, pb, mn, ni, zn, sn, fe, cd and co. trace metal contents of fruit juices were f ...200918704729
evaluation of crude sesquiterpenoid extract of phellinus fastuosus as a natural preservative.it has been revealed that the use of synthetic food preservatives has variety of various side effects and hence search of safe and effective natural preservative has become a need. with the afore said objective the present study to screen the possibility of using sesquiterpenoid extract of local folk mushroom phellinus fastuosus as food preservative was undertaken. microbial growth inhibition by sesquiterpenoid extract of phellinus was evaluated in the media with natural ingredient such as milk ...201318697727
carotenoid production by xanthophyllomyces dendrorhous: use of pineapple juice as a production medium.to select a carotenoid-hyperproducing mutant and to formulate pineapple juice as a carotenoid production medium.200818673431
effect of bromelain on milk yield, milk composition and mammary health in dairy goats.a 7 month prospective cohort study was designed to determine if feeding bromelain to dairy goats influenced the mscc, milk yield, milk composition and the incidence of imi. forty-four clinically normal goats from 2nd to 6th parities were studied. daily bromelain dosage was 7.4 grams/animal (185-mg/kg weight). samples for diagnostic bacteriology were collected from each udder half every 2 weeks. samples for mscc and composition were obtained every 42 days. milk yield was also recorded every 42 da ...200918661260
the effects of concentrate added to pineapple (ananas comosus linn. mer.) waste silage in differing ratios to form complete diets, on digestion, excretion of urinary purine derivatives and blood metabolites in growing, male, thai swamp buffaloes.four, male, growing thai swamp buffaloes (197 +/- 5.3 kg and all 1 year old) were used to evaluate the effects of concentrate added to pineapple waste silage in differing ratios, to form a complete diet, studying in vivo digestion, the rate of passage, microbial protein synthesis and blood metabolites. animals were fed ad libitum with 4 diets, using four combinations of pineapple waste silage (p) and concentrate (c), in the proportions (on a dry matter basis) of 0.8:0.2 (p80:c20), 0.6:0.4 (p60:c ...200918649000
identification of viral and non-viral reverse transcribing elements in pineapple (ananas comosus), including members of two new badnavirus species.a previously published partial sequence of pineapple bacilliform virus was shown to be from a retrotransposon (family metaviridae) and not from a badnavirus as previously thought. two newly discovered sequence groups isolated from pineapple were associated with bacilliform virions and were transmitted by mealybugs. phylogenetic analyses indicated that they were members of new badnavirus species. a third caulimovirid sequence was also amplified from pineapple, but available evidence suggests that ...200818607672
multiresidue method for fast determination of pesticides in fruit juices by ultra performance liquid chromatography coupled to tandem mass spectrometry.a new analytical method for the simultaneous determination of 90 pesticides in fruit juices by ultra performance liquid chromatography coupled to tandem mass spectrometry (uplc-ms/ms) has been developed and validated. extraction was performed with acetonitrile, applying quechers methodology, and the extracts were analyzed without any further clean-up step, providing better results than solid phase extraction (spe) procedure. before chromatographic step, extracts were diluted with water (1:1) in ...200818585265
assessment of citrate concentrations in citrus fruit-based juices and beverages: implications for management of hypocitraturic nephrolithiasis.dietary intake of citrate in the form of citrus juices (eg, lemonade, orange juice) will enhance urinary citrate excretion, a valuable benefit for patients with hypocitraturic calcium oxalate nephrolithiasis. while information on citrate concentrations in select citrus juices is available, data on citrate concentrations of commercially available beverages (juice and otherwise) are limited. using nuclear magnetic resonance spectroscopy (1h nmr), we report citrate concentrations of several beverag ...200818578663
a novel agricultural waste adsorbent for the removal of cationic dye from aqueous solutions.in this paper, pineapple stem (ps) waste, an agricultural waste available in large quantity in malaysia, was utilized as low-cost adsorbent to remove basic dye (methylene blue, mb) from aqueous solution by adsorption. batch mode experiments were conducted at 30 degrees c to study the effects of initial concentration of methylene blue, contact time and ph on dye adsorption. equilibrium adsorption isotherms and kinetic were investigated. the experimental data were analyzed by the langmuir and freu ...200918573607
continuous growth kinetics of candida utilis in pineapple cannery effluent.candida utilis was grown on a pineapple cannery effluent as the sole carbon and energy source in a chemostat at dilution rates between 0.10 and 0.62 h(-1) to determine the growth kinetics. the principal sugars in the effluent were sucrose, glucose, and fructose. the cell yield coefficient on carbohydrate varied with dilution rate and a maximum value of 0.63 was observed at a dilution rate of 0.33 h(-1). the steady-state concentrations of carbohydrate, reducing sugar, and chemical oxygen demand ( ...198418553441
crystal structures of saturn-like c50cl10 and pineapple-shaped c64cl4: geometric implications of double- and triple-pentagon-fused chlorofullerenes. 200818548468
survival of sa11 rotavirus in fresh fruit juices of pineapple, papaya, and honeydew melon.survival of rotavirus in fresh fruit juices of papaya (caraca papaya l.), honeydew melon (cucumis melo l.), and pineapple (ananas comosus [l.] merr.) was studied. clarified juices were prepared from pulps of ripe fruits and sterilized by ultrafiltration. one milliliter of juice from each fruit was inoculated with 20 microl of 1 x 10(6) pfu of sa11 rotavirus and sampled immediately (0-h exposure) and 1 and 3 h later at 28 degrees c. mean viral titers in juices of papaya (ph 5.1) and honeydew melo ...200818522042
profilin sensitization detected in the office by skin prick test: a study of prevalence and clinical relevance of profilin as a plant food allergen.profilin, a pan-allergen present in all eukaryotic cells, is one of the main causes of cross-sensitization between pollen and plant-derived foods, but its clinical relevance as a food allergen is still debated.200818489029
bromelain treatment decreases neutrophil migration to sites of inflammation.bromelain, a mixture of proteases derived from pineapple stem, has been reported to have therapeutic benefits in a variety of inflammatory diseases, including murine inflammatory bowel disease. the purpose of this work was to understand potential mechanisms for this anti-inflammatory activity. exposure to bromelain in vitro has been shown to remove a number of cell surface molecules that are vital to leukocyte trafficking, including cd128a/cxcr1 and cd128b/cxcr2 that serve as receptors for the n ...200818482869
ultrasound as pre-treatment for drying of pineapple.dehydration of fruits is an alternative to reduce post-harvest loss of fruits and also a process to produce dried fruits, which can be directly consumed or become part of foodstuffs like cakes, pastries and many others. the effect of ultrasonic pre-treatment and ultrasound-assisted osmotic dehydration, before air-drying, on dehydration of pineapple (ananas comosus) was investigated. this study allowed estimating the water diffusivity in the air-drying process for pineapples submitted to ultrasou ...200818462985
inactivation of escherichia coli and listeria innocua in kiwifruit and pineapple juices by high hydrostatic pressure.escherichia coli and listeria innocua in kiwifruit and pineapple juices were exposed to high hydrostatic pressure (hhp) at 300 mpa for 5 min. both bacteria showed equal resistance to hhp. using low (0 degrees c) or sub-zero (-10 degrees c) temperatures instead of room temperature (20 degrees c) during pressurization did not change the effectiveness of hhp treatment on both bacteria in studied juices. pulse pressure treatment (multiple pulses for a total holding time of 5 min at 300 mpa) instead ...200818455820
bacterial cellulose production by acetobacter xylinum strains from agricultural waste products.bacterial cellulose is a biopolysaccharide produced from the bacteria, acetobacter xylinum. static batch fermentations for bacterial cellulose production were studied in coconut and pineapple juices under 30 degrees c in 5-l fermenters by using three acetobacter strains: a. xylinum tistr 998, a. xylinum tistr 975, and a. xylinum tistr 893. experiments were carried out to compare bacterial cellulose yields along with growth kinetic analysis. results showed that a. xylinum tistr 998 produced a bac ...200818418756
cloning, localization and expression analysis of vacuolar sugar transporters in the cam plant ananas comosus (pineapple).in photosynthetic tissues of the cam plant pineapple (ananas comosus), storage of soluble sugars in the central vacuole during the daytime and their remobilization at night is required to provide carbon skeletons for nocturnal co(2) fixation. however, soluble sugars produced photosynthetically must also be exported to support growth processes in heterotrophic tissues. to begin to address how vacuolar sugar storage and assimilate partitioning are regulated in a. comosus, degenerate pcr and cdna l ...200818408220
malate metabolism in hoya carnosa mitochondria and its role in photosynthesis during cam phase iii.this study investigated the respiratory properties and the role of the mitochondria isolated from one phosphoenolpyruvate carboxykinase (pck)-cam plant, hoya carnosa, in malate metabolism during cam phase iii. the mitochondria showed high malate dehydrogenase (mmdh) and aspartate amino transferase (mast), and a significant amount of malic enzyme (mme) activities. h. carnosa readily oxidized malate via mme and mmdh in the presence of some cofactors such as thiamine pyrophosphate (tpp), coenzyme a ...200818403382
movement of bromacil in a hawaii soil under pineapple cultivation - a field study.recent discovery of low concentrations of bromacil in drinking water prompted the state of hawaii to examine the leaching behavior of bromacil in pineapple fields. this study is a follow up to earlier work on bromacil concentrations in soil profiles in a pineapple field in central oahu, hawaii. soil samples were collected for bromacil analysis at different times prior to and after application from a pineapple field that was previously surveyed by other research workers. the leaching pattern of b ...200818374966
glycemic index of common malaysian fruits.the objective of the present study was to measure the glycemic index of durian, papaya, pineapple and water-melon grown in malaysia. ten (10) healthy volunteers (5 females, 5 males; body mass index 21.18+/-1.7 kg/m2) consumed 50 g of available carbohydrate portions of glucose (reference food) and four test foods (durian, papaya, pineapple and watermelon) in random order after an overnight fast. glucose was tested on three separate occasions, and the test foods were each tested once. postprandial ...200818364324
profiling of different bioactive compounds in functional drinks by high-performance liquid chromatography.in the present work, an hplc method is proposed to simultaneously detect and quantify water- and fat-soluble vitamins, phenolic compounds, carotenoids and chlorophylls in a single run, by using an ultradeactivated c18 column and gradient separation using trifluoroacetic acid, water and methanol. it is shown that the hplc method provides baseline separation of all these compounds with good resolution values in 40 min. moreover, other figures of merit of the method show a good linear response and ...200818325525
evaluation of selection strategies alternative to nptii in genetic transformation of citrus.the neomycin phosphotransferase (nptii) selection system has proved successful in citrus transformation; however, it may be recommendable to replace it given the pressure exerted against antibiotic-resistance selectable marker genes in transgenic plants. the present work investigates three different selection alternatives, comparing them to nptii selection in two citrus genotypes, carrizo citrange and pineapple sweet orange. the first method used the beta-glucuronidase (uida) reporter marker gen ...200818317775
oral bromelain attenuates inflammation in an ovalbumin-induced murine model of asthma.bromelain, a widely used pineapple extract with cysteine protease activity, has been shown to have immunomodulatory effects in a variety of immune system models. the purpose of the present study was to determine the effects of orally administered bromelain in an ovalbumin (ova)-induced murine model of acute allergic airway disease (aad). to establish aad, female c57bl/6j mice were sensitized with intraperitoneal (i.p.) ova/alum and then challenged with ova aerosols for 3 days. mice were gavaged ...200818317550
just noticeable differences in component concentrations modify the odor quality of a blending mixture.the odors we perceive are mainly the result of mixtures of odorants that, however, are commonly perceived as single undivided entities; nevertheless, the processes involved remain poorly explored. it has been recently reported that perceptual blending based on configural olfactory processing can cause odorant mixtures to give rise to an emergent odor not present in the components. the present study examined whether specific component proportions are required to elicit an emergent odor. starting ...200818304991
effect of ionizing radiation on furan formation in fresh-cut fruits and vegetables.furan, a possible carcinogen, is commonly induced by thermal processing in a wide variety of foods. the possible formation of furan from fresh-cut fruits and vegetables due to irradiation was studied. nineteen fresh-cut fruits and vegetables were irradiated by 5 kgy gamma rays at 4 degrees c. furan was analyzed from the irradiated samples using a number of preparation methods. the results showed that almost all tested fruits and vegetables, upon irradiation, produced nondetectable levels, or les ...200818298720
complete nucleotide sequence and genome organization of pineapple mealybug wilt-associated virus-1.pineapple mealybug wilt-associated virus-1 (pmwav-1; family closteroviridae, genus ampelovirus) belongs to a complex of mealybug-transmissible viruses found in pineapple worldwide. in this study, the complete genome of pmwav-1 was sequenced and found to be 13.1 kb in length, making it the smallest in the family. the genome encoded seven open reading frames (orfs) and was unusual for an ampelovirus due to the lack of an intergenic region between the rdrp and p6 orfs, an orf encoding a relatively ...200818283409
inhibitory effects of fruit juices on cytochrome p450 2c9 activity in vitro.there is limited information on the effect of fruits on human cytochrome p450 (cyp) 2c9 activity. the objective of this study was to determine the effect of fruit juice on cyp2c9-mediated drug metabolism. nine citrus fruits and eight tropical fruits were chosen. we investigated effects of the fruits on diclofenac 4'-hydroxylation and tolbutamide hydroxylation by human liver microsomes. among the fruits, pineapple juice showed potent inhibition of cyp2c9 activity. the addition of 25 microl (5.0% ...200818256496
bromelain induces cardioprotection against ischemia-reperfusion injury through akt/foxo pathway in rat myocardium.bromelain (br), a proteolytic enzyme extracted from the stem of the pineapple, is known to possess anti-inflammatory activity and has been shown to reduce blood viscosity, prevent the aggregation of blood platelets, and improve ischemia-reperfusion (i/r) injury in a skeletal muscle model. we investigated the capacity of br to limit myocardial injury in a global i/r model. adult male sprague-dawley rats were divided into two groups: control (pbs) and br at 10 mg/kg in pbs administered via intrape ...200818192224
the biosynthesis of auxin in the vegetative pineapple; the precursors of indoleacetic acid. 194918108931
the biosynthesis of auxin in the vegetative pineapple; nature of the active auxin. 194918108930
[confirmation of a woodborer as a pest of pineapple]. 194818099936
perceptual processing strategy and exposure influence the perception of odor mixtures.in flavor perception, both experience with the components of odor/taste mixtures and the cognitive strategy used to examine the interactions between the components influence the overall mixture perception. however, the effect of these factors on odor mixtures perception has never been studied. the present study aimed at evaluating whether 1) previous exposure to the odorants included in a mixture or 2) the synthetic or analytic strategy engaged during odorants mixture evaluation determines odor ...200818071197
[pineapple juice as a negative oral contrast agent in magnetic resonance cholangiopancreatography].the quality of magnetic resonance cholangiopancreatography (mrcp) images is frequently degraded by high signal from the gastrointestinal tract on heavily t2w images. the purpose of this study is to evaluate pineapple juice (pj) as an oral negative contrast agent in mrcp.200718065928
carotenoids and color of fruit juice and milk beverage mixtures.seventeen commercially available, "ready to drink" fortified beverages consisting of mixtures of fruit juices and milk were analyzed to evaluate their carotenoid profile (including their z/e stereoisomers) and color during their commercial shelf life. lightness (l*) was found to be correlated with the content of milk in the mixtures (r= 0.649) whereas red-yellow colors were correlated with the contents of alpha-carotene, beta-cryptoxanthin, and beta-carotene supplied by the fruit. the beverages ...200718034704
in vitro anthelmintic effects of cysteine proteinases from plants against intestinal helminths of rodents.infections with gastrointestinal (gi) nematodes are amongst the most prevalent worldwide, especially in tropical climates. control of these infections is primarily through treatment with anthelmintic drugs, but the rapid development of resistance to all the currently available classes of anthelmintic means that alternative treatments are urgently required. cysteine proteinases from plants such as papaya, pineapple and fig are known to be substantially effective against three rodent gi nematodes, ...200718005461
inhibitory effect of fruit extracts on the formation of heterocyclic amines.natural extracts have attracted considerable attention for development into effective inhibitors against the formation of genotoxic heterocyclic amines (has) in processed foods. in this study, four fruit extracts (apple, elderberry, grape seed, and pineapple) were evaluated for their effects on ha formation in fried beef patties. apple and grape seed extracts were found to be the most effective in both the degree of inhibition in the formation of individual has (2-amino-3,8-dimethylimidazo [4,5- ...200718004801
effectiveness of radiation processing for elimination of salmonella typhimurium from minimally processed pineapple (ananas comosus merr.).the microbiological quality of market samples of minimally processed (mp) pineapple was examined. the effectiveness of radiation treatment in eliminating salmonella typhimurium from laboratory inoculated ready-to-eat pineapple slices was also studied. microbiological quality of minimally processed pineapple samples from mumbai market was poor; 8.8% of the samples were positive for salmonella. d(10) (the radiation dose required to reduce bacterial population by 90%) value for s. typhimurium inocu ...200717995808
a comparison of nutrient density scores for 100% fruit juices.the 2005 dietary guidelines for americans recommend that consumers choose a variety of nutrient-dense foods. nutrient density is usually defined as the quantity of nutrients per calorie. food and nutrition professionals should be aware of the concept of nutrient density, how it might be quantified, and its potential application in food labeling and dietary guidance. this article presents the concept of a nutrient density score and compares nutrient density scores for various 100% fruit juices. o ...200717995788
quantification of selected aroma-active compounds in strawberries by headspace solid-phase microextraction gas chromatography and correlation with sensory descriptive analysis.selected aroma-active compounds in strawberries were quantified using headspace solid-phase microextraction and gas chromatography. ten strawberry cultivars grown in california and oregon were studied. the standard curves were built in a synthetic matrix and quantification was achieved using multiple internal standards. odor activity values (oavs) of the aroma compounds were calculated to understand their contribution to the overall aroma. although the concentrations of the aroma compounds varie ...200717995662
use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes.reverse micellar systems of ctab/isooctane/hexanol/butanol and aot/isooctane are used for the extraction and primary purification of bromelain from crude aqueous extract of pineapple wastes (core, peel, crown and extended stem). the effect of forward as well as back extraction process parameters on the extraction efficiency, activity recovery and purification fold is studied in detail for the pineapple core extract. the optimized conditions for the extraction from core resulted in forward and ba ...200817964777
heavy metals removal from contaminated sewage sludge by naturally fermented raw liquid from pineapple wastes.the large amount of unutilised pineapple wastes produced every year in tropical countries, particularly in thailand, adds to the existing environmental pollution problems of the country. this study investigated the utilisation of pineapple wastes to treat another form of waste (sludge) from wastewater treatment facilities in thailand. laboratory scale studies were carried out to determine the potential of using naturally fermented raw liquid from pineapple wastes as a source of citric acid in th ...200717951878
application of statistical experimental designs for the optimization of medium constituents for the production of citric acid from pineapple waste.statistical experimental designs were applied for the optimization of medium constituents for citric acid production by yarrowia lipolytica ncim 3589 in solid state fermentation (ssf) using pineapple waste as the sole substrate. using plackett-burman design, yeast extract, moisture content of the substrate, kh(2)po(4) and na(2)hpo(4) were identified as significant variables which highly influenced citric acid production and these variables were subsequently optimized using a central composite de ...200817936623
in vivo antitumoral activity of stem pineapple (ananas comosus) bromelain.stem bromelain (ec 3.4.22.32) is a major cysteine proteinase, isolated from pineapple ( ananas comosus) stem. its main medicinal use is recognized as digestive, in vaccine formulation, antitumoral and skin debrider for the treatment of burns. to verify the identity of the principle in stem fractions responsible for the antitumoral effect, we isolated bromelain to probe its pharmacological effects. the isolated bromelain was obtained from stems of adult pineapple plants by buffered aqueous extrac ...200717893836
anthelmintic inactivity of fresh pineapple juice in vivo. 194017831178
flower formation in the pineapple plant as controlled by 2,4-d and naphthaleneacetic acid. 194517818200
anthelmintic activity of fresh pineapple juice. 193917809477
report on wax from several species of tillandsia and from ananas comosus (l.) merr. 195517801092
toxicity to pineapple plants of biuret found in urea fertilizers from different sources. 195417753560
photosynthetic pathway and biomass energy production.the current interest in locating new or alternative sources of energy has focused attention on solar energy capture by crops that can be subsequently utilized as a substitute for fossil fuels. the very high productivity of sugarepane and the fact that it accumulates sugars that are directly fermentable to alcohol may have caused seemingly less productive crops to be overlooked. we show here that recoverable alcohol from achievable commercial yields of pineapple can actually equal that of sugarca ...197917729660
characterization of active phenolic components in the ethanolic extract of ananas comosus l. leaves using high-performance liquid chromatography with diode array detection and tandem mass spectrometry.hplc-dad-ms was utilized to investigate the phytochemical constituents in ethanolic extract of ananas comosus l. leaves (eeacl) responsible for antidiabetic, antihyperlipidemic and antioxidative effects. eight phenylpropane diglycerides, together with two hydroxycinnamic acids, three hydroxycinnamoyl quinic acids, four phenylpropane monoglycerides, three flavones and six phenylpropanoid glycosides were detected, and their proposed structures were elucidated based on hplc retention time, uv and m ...200717698075
role of bromelain in the treatment of patients with pityriasis lichenoides chronica.pityriasis lichenoides chronica (plc) is a skin disease of unknown etiology. uncertainty about the etiopathogenesis of this skin disease is the reason for the unpredictable and non optimal efficacy of therapies available for its treatment. the aim of the present study was to evaluate the efficacy of bromelain, a crude aqueous extract of the stems and immature fruit of pineapple, in the treatment of plc.200717671882
determination of neutral carbohydrates by cze with direct uv detection.a new cze method relying on in-capillary reaction and direct uv detection at the wavelength 270 nm is presented for the simultaneous separation of the neutral carbohydrates xylitol, d-(-)-mannitol, sucrose, d-(+)-fucose, d-(+)-cellobiose, d-(+)-galactose, d-(+)-glucose, l-rhamnose, d-(+)-mannose, d-(-)-arabinose, d-(+)-xylose, and d-(-)-ribose. the alkaline electrolyte solution was prepared of 130 mm sodium hydroxide and 36 mm disodium hydrogen phosphate dihydrate. separation of the sample mixtu ...200717661315
nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes.cysteine proteinases from the fruit and latex of plants, such as papaya, pineapple and fig, have previously been shown to have substantial anthelmintic efficacy, in vitro and in vivo, against a range of animal parasitic nematodes. in this paper, we describe the in vitro effects of these plant extracts against 2 sedentary plant parasitic nematodes of the genera meloidogyne and globodera. all the plant extracts examined caused digestion of the cuticle and decreased the activity of the tested nemat ...200717640402
[determination of 1, 3-o-dicaffeoylglycerides from leaves of pineapple by hplc].to establish a hplc method for a new compound 1,3-o-dicaffeoylglycerides determination.200717639981
functional characterization of enone oxidoreductases from strawberry and tomato fruit.fragaria x ananassa enone oxidoreductase (faeo), earlier putatively assigned as quinone oxidoreductase, is a ripening-induced, negatively auxin-regulated enzyme that catalyzes the formation of 4-hydroxy-2,5-dimethyl-3(2h)-furanone (hdmf), the key flavor compound in strawberry fruit by the reduction of the alpha,beta-unsaturated bond of the highly reactive precursor 4-hydroxy-5-methyl-2-methylene-3(2h)-furanone (hmmf). here we show that recombinant faeo does not reduce the double bond of straight ...200717636940
microbial control of arthropod pests of tropical tree fruits.a multitude of insects and mites attack fruit crops throughout the tropics. the traditional method for controlling most of these pests is the application of chemical pesticides. growing concern on the negative environmental effects has encouraged the development of alternatives. inundatively and inoculatively applied microbial control agents (virus, bacteria, fungi, and entomopathogenic nematodes) have been developed as alternative control methods of a wide variety of arthropods including tropic ...200717607448
enhanced separation of membranes during free flow zonal electrophoresis in plants.free flow zonal electrophoresis (ffze) is a versatile technique that allows for the separation of cells, organelles, membranes, and proteins based on net surface charge during laminar flow through a thin aqueous layer. we have been optimizing the ffze technique to enhance separation of plant vacuolar membranes (tonoplast) from other endomembranes to pursue a directed proteomics approach to identify novel tonoplast transporters. addition of atp to a mixture of endomembranes selectively enhanced e ...200717566980
cytokinins and auxin communicate nitrogen availability as long-distance signal molecules in pineapple (ananas comosus).this work aimed at identifying a possible role of phytohormones in long-distance (root-shoot) signaling under nitrogen deficiency. three-months old pineapple plants were transferred from murashige and skoog (ms) medium to nitrogen-free ms (-n). during the first 24h on -n, 20 plants were harvested every 4h. after 30 days in -n, the remaining plants were transferred back to regular ms (+n) and 20 plants harvested every 4h for the first 24h. following the harvests, endogenous levels of nitrate (no( ...200717566606
removal of heavy metals from contaminated sewage sludge using aspergillus niger fermented raw liquid from pineapple wastes.the environmental benefits derived from using citric acid in the removal of heavy metals from contaminated sewage sludge have made it promising as an extracting agent in the chemical extraction process. at present, citric acid is produced commercially by fermentation of sucrose using mutant strains of aspergillus niger (a. niger), and chemical synthesis. in recent years, various carbohydrates and wastes (such as pineapple wastes) have been considered experimentally, to produce citric acid by a. ...200817512728
[new cerebroside from leaves of pineapple].to study the chemical constituents of the leaves of pineapple.200717511144
detection of some safe plant-derived foods for ltp-allergic patients.lipid transfer protein (ltp) is a widely cross-reacting plant pan-allergen. adverse reactions to rosaceae, tree nuts, peanut, beer, maize, mustard, asparagus, grapes, mulberry, cabbage, dates, orange, fig, kiwi, lupine, fennel, celery, tomato, eggplant, lettuce, chestnut and pineapple have been recorded.200717505138
production of butyl acetate ester by lipase from novel strain of rhizopus oryzae.a new lipase preparation from rhizopus oryzae was used to catalyze the esterification reaction between acetic acid and butanol to produce butyl acetate ester (pineapple flavor). this flavor compound can be used in food, cosmetic and pharmaceutical industries. only 3% of butyl acetate was obtained when free lipase was used in the synthesis containing only the substrates. in contrast, the conversion yield reached 25% when immobilized lipase was used under the same conditions. the synthesis of buty ...200717502279
pineapple juice and its fractions in enzymatic browning inhibition of banana [musa (aaa group) gros michel].the effectiveness of pineapple juice in enzymatic browning inhibition was evaluated on the cut surface of banana slices. after storage of banana slices at 15 degrees c for 3 days, pineapple juice showed browning inhibition to a similar extent as 8 mm ascorbic acid but less than 4 mm sodium metabisulfite. fractionation of pineapple juice by a solid-phase c18 cartridge revealed that the directly eluted fraction (de fraction) inhibited banana polyphenol oxidase (ppo) about 100% when compared to the ...200717439237
disease regression in malignant melanoma: spontaneous resolution or a result of treatment with antioxidants, green tea, and pineapple cores? a case report. 200717393612
hypolipidemic mechanisms of ananas comosus l. leaves in mice: different from fibrates but similar to statins.in this study, we investigated hypolipidemic mechanisms of the ethanolic extract of ananas comosus l. leaves (ac) in mice and then determined its activities in related enzymes. the results showed that ac (0.40 g/kg) significantly inhibited the increase in serum triglycerides by 40% in fructose-fed mice. in mice induced by alloxan and high-fat diets, serum total cholesterol remained at a high level (180 - 220 mg/dl) within 7 days of removing high-fat diets but reached normal level (120 - 140 mg/d ...200717380035
biological detoxification of cr(vi) using wood-husk immobilized acinetobacter haemolyticus.acinetobacter haemolyticus, a gram-negative aerobic locally isolated bacterium, immobilized on wood-husk showed the ability to detoxify cr(vi) to cr(iii). wood-husk, a natural cellulose-based support material, packed in an upward-flow column was used as support material for bacterial attachment. around 97% of the cr(vi) in wastewater containing 15 mg l(-1) of cr(vi) was reduced at a flow rate of 8.0 ml min(-1). the wastewater containing cr(vi) was added with liquid pineapple wastewater as nutrie ...200717368716
inhibition of tumour growth in vitro by bromelain, an extract of the pineapple plant (ananas comosus). 198517345291
[occupational allergies to bromelain].the protease bromelain originating from the pineapple fruit (ananas comosus) finds frequent use in industry. exposure to enzyme dusts has long been known to cause occupational allergies. the present paper reviews the results of the evaluation of literature data concerning occupational airway sensitization due to bromelain. cases of specific airway sensitization caused by bromelain could be shown clearly by the presented studies. since the symptoms, results of skin prick tests, detection of speci ...200717342576
genetic component of identification, intensity and pleasantness of odours: a finnish family study.although potential odorant receptor genes have been identified, the precise genetic component of perception of odours is still obscure. although there is some evidence for heritability of a few olfactory-related traits, no genome-wide search for loci harboring underlying genes has been published to date. we performed a genome-wide scan to identify loci affecting the identification, intensity and pleasantness of 12 odours (cinnamon, turpentine, lemon, smoke, chocolate, rose, paint thinner, banana ...200717342154
prooxidant action of furanone compounds: implication of reactive oxygen species in the metal-dependent strand breaks and the formation of 8-hydroxy-2'-deoxyguanosine in dna.prooxidant properties of furanone compounds including 2,5-furanone (furaneol, 4-hydroxy-2,5-dimethyl-furan-3-one), 4,5-furanone (4,5-dimethyl-3-hydroxy-2(5h)-furanone) (sotolone) and cyclotene (2-hydroxy-3-methyl-2-cyclopenten-1-one) were analyzed in relation to the metal-reducing activity. only 2.5-furanone known as a "strawberry or pineapple furanone" inactivated aconitase the most sensitive enzyme to active oxygen in the presence of ferrous sulfate, suggesting the furaneol/iron-mediated gener ...200717316945
removal of necrotic tissue with an ananain-based enzyme-debriding preparation.an enzymatic debriding preparation was formulated with purified enzyme derived from a crude pineapple stem extract. the primary component of this preparation was the sulfhydryl protease ananain which represented >/=85% of the proteolytic activity. the remaining proteolytic activity in the preparation was contributed by a co-purifying homologous cysteine protease comosain. taken together these two proteases provided a protein purity of greater than 95% as judged by sodium dodecyl sulfate polyacry ...199617309694
a synthetic analog of plantaricin 149 inhibiting food-borne pathogenic bacteria:evidence for alpha-helical conformation involved in bacteria-membrane interaction.plantaricin-149 is a bacteriocin produced by lactobacillus plantarum nric 149 (a lab isolated from pineapple), which consists of a peptidic chain made up of 22 amino acid residues [kato et al. j. ferment. bioeng. 1994; 77: 277-282]. in this work, a synthetic c-terminal amidated peptide analog denoted pln149a was prepared by spps-fmoc chemistry and the antagonistic activity against gram-positive and gram-negative bacteria was tested. the secondary structure was studied by circular dichroism (cd) ...200717266050
quantification of spiroether isomers and herniarin of different parts of matricaria matricarioides and flowers of chamaemelum nobile.a simple hplc-pad-ms method was established to quantitatively analyse two spiroether isomers (cis-en-yn-dicycloether and trans-en-yn-dicycloether) and the main coumarin, herniarin, in chamomile herbs, simultaneously. by using this method, the contents of these three compounds in the flowers of two chamomile species, roman chamomile (chamaemelum nobile) and pineapple weed (matricaria matricarioides), as well as in different parts of pineapple weed, were investigated. it was found that the flowers ...201217260697
[role of green tea in oxidative stress prevention].oxidative stress is a condition caused by an increase of reactive oxygen species (ros) or by a shortage of the mechanisms of cellular protection and antioxidant defence. ros have a potential oxidative effect towards various cellular macromolecules: proteins, nucleic acids, proteoglycans, lipids, with consequent damages in several cellular districts and promotion of the ageing process of the organism. however, some substances are able to prevent and/or reduce the damages caused by ros; therefore, ...200717228849
improved detection of sugar addition to maple syrup using malic acid as internal standard and in 13c isotope ratio mass spectrometry (irms).stable carbon isotope ratio mass spectrometry (delta13c irms) was used to detect maple syrup adulteration by exogenous sugar addition (beet and cane sugar). malic acid present in maple syrup is proposed as an isotopic internal standard to improve actual adulteration detection levels. a lead precipitation method has been modified to isolate quantitatively malic acid from maple syrup using preparative reversed-phase liquid chromatography. the stable carbon isotopic ratio of malic acid isolated fro ...200717227042
herbal remedy in the treatment of malaria: cross sectional survey of residents of lagos state, nigeria.semi structured questionnaires. designed to capture information on the type. composition, method of preparation. dosage, mode of administration. and frequency of use of herbal preparations in malaria treatment, were administered to 1,593 adults of the 3 main ethnic groups and a forth group comprising other smaller ethnic groups designated as "others", all resident in lagos metropolis in a cross sectional survey. the 1,593 respondents were made up of 892 males and 701 females and their ages range ...200617209310
the growth of propionibacterium cyclohexanicum in fruit juices and its survival following elevated temperature treatments.this study investigated the growth of propionibacterium cyclohexanicum in orange juice over a temperature range from 4 to 40 degrees c and its ability to multiply in tomato, grapefruit, apple, pineapple and cranberry juices at 30 and 35 degrees c. survival after 10 min exposure to 50, 60, 70, 80, 85, 90 and 95 degrees c in culture medium and in orange juice was also assessed. in orange juice the organism was able to multiply by 2 logs at temperatures from 4 to 35 degrees c and survived for up to ...200717189756
oral immunogenicity of the plant proteinase bromelain.bromelain is a natural mixture of proteolytic enzymes derived from pineapple stem that has been shown to have anti-inflammatory activity when administered orally. although most proteins given orally without adjuvant (e.g., food) result in tolerance, we previously reported that long-term oral exposure to bromelain stimulated the development of high serum anti-bromelain antibody titers. the purpose of these studies was to further investigate the mechanisms responsible for the immunogenicity of ora ...200617161360
sensory-specific satiety with simple foods in humans: no influence of bmi?olfacto-gustatory sensory-specific satiety plays an important role in the termination of food ingestion. a defect in this mechanism, by increasing food intake, could be a factor in development of overweight. the present study was conducted to explore whether sensory-specific satiety in the overweight may be different from that in normal-weight subjects.200717160089
transcriptional regulation of a pineapple polyphenol oxidase gene and its relationship to blackheart.two genes encoding polyphenol oxidase (ppo) were isolated from pineapple (ananas comosus[l.] merr. cv. smooth cayenne). sequence analyses showed that both contained a single intron and encoded typical chloroplast-localized ppo proteins, the sequences of which corresponded to two pineapple ppo cdnas, pinppo1 and pinppo2, recently described by stewart et al. (2001). southern blot analyses suggested that pineapple contained only two ppo genes. analysis of expression of pinppo1 promoter gus fusion c ...200317134404
'complementary ent': a systematic review of commonly used supplements.to assess the evidence surrounding the use of certain complementary supplements in otolaryngology. we specifically focussed on four commonly used supplements: spirulina, ginkgo biloba, vertigoheel and nutritional supplements (cod liver oil, multivitamins and pineapple enzyme).200717125579
bromelain as an adjunctive treatment for moderate-to-severe osteoarthritis of the knee: a randomized placebo-controlled pilot study.osteoarthritis (oa) of the knee is the most prevalent joint disorder. previous studies suggest that bromelain, a pineapple extract, may be a safer alternative/adjunctive treatment for knee oa than current conventional treatment.200617121765
cine magnetic resonance imaging of the small bowel: comparison of different oral contrast media.to evaluate several substances regarding small bowel distension and contrast on balanced steady-state free precession (bssfp) cine magnetic resonance (mr) images.200617077038
deforestation alters phytotelm habitat availability and mosquito production in the peruvian amazon.we quantified the effects of deforestation, and subsequent cultivation and forest regeneration, on the abundance and composition of mosquito larval habitats, specifically phytotelmata (plant-held waters), in the western amazon basin. recently deforested sites were characterized by increased phytotelm density (1.6 phytotelmata/m2) and greater relative abundance of fallen-plant-part phytotelmata (76%) compared to intact forests (0.9 phytotelmata/m2 and 25% fallen plant parts). as a result, the tot ...200617069377
[studies on phenolic constituents from leaves of pineapple (ananas comosus)].to study the phenolic constituents of the leaves of pineapple.200617048566
silencing of the acc synthase gene acacs2 causes delayed flowering in pineapple [ananas comosus (l.) merr.].flowering is a crucial developmental stage in the plant life cycle. a number of different factors, from environmental to chemical, can trigger flowering. in pineapple, and other bromeliads, it has been proposed that flowering is triggered by a small burst of ethylene production in the meristem in response to environmental cues. a 1-amino-cyclopropane-1-carboxylate synthase (acc synthase) gene has been cloned from pineapple (acacs2), which is induced in the meristem under the same environmental c ...200617046980
pineapple [ananas comosus (l.) merr].a procedure for pineapple [ananas comosus (l.) merr.] genetic transformation is described, which involves temporary immersion bioreactors (tib) for selection of transgenic plants. success in the production of transgenic pineapple plants combines tissue culture factors. firstly, the use of regenerable pineapple callus as starting material for transformation whose cells shown to be competent for agrobacterium infection. secondly, the used of filtered callus, resulting in homogeneously sized cluste ...200617033065
anthelmintic action of plant cysteine proteinases against the rodent stomach nematode, protospirura muricola, in vitro and in vivo.cysteine proteinases from the fruit and latex of plants, including papaya, pineapple and fig, were previously shown to have a rapid detrimental effect, in vitro, against the rodent gastrointestinal nematodes, heligmosomoides polygyrus (which is found in the anterior small intestine) and trichuris muris (which resides in the caecum). proteinases in the crude latex of papaya also showed anthelmintic efficacy against both nematodes in vivo. in this paper, we describe the in vitro and in vivo effect ...200717032468
the complete chloroplast genome sequence of citrus sinensis (l.) osbeck var 'ridge pineapple': organization and phylogenetic relationships to other angiosperms.the production of citrus, the largest fruit crop of international economic value, has recently been imperiled due to the introduction of the bacterial disease citrus canker. no significant improvements have been made to combat this disease by plant breeding and nuclear transgenic approaches. chloroplast genetic engineering has a number of advantages over nuclear transformation; it not only increases transgene expression but also facilitates transgene containment, which is one of the major impedi ...200617010212
determination of isopropyl thioxanthone (itx) in fruit juices by pressurized liquid extraction and liquid chromatography-mass spectrometry.a rapid lc-ms method, which compares different mass analyzers-single quadrupole, ion trap, and triple quadrupole-was developed for the quantitative determination of isopropyl thioxanthone (itx) in fruit juices. itx, a photoinitiator in uv-cured inks that can reach foods from the cartons for beverages in which they are used, was extracted from fruit juice samples with acetone/hexane (50:50) using pressurized liquid extraction. this method gave detection limits of 3, 3, and 0.01 microg/l and quant ...200617002475
on the proteolytic action of bromelin, the ferment of pineapple juice. 189316992096
moulds and yeasts in fruit salads and fruit juices.thirty-eight fruit salad samples including cantaloupe, citrus fruits, honeydew, pineapple, cut strawberries and mixed fruit salads, and 65 pasteurized fruit juice samples (apple, carrot, grapefruit, grape and orange juices, apple cider, and soy milk) were purchased from local supermarkets in the washington, dc area and tested for fungal contamination. the majority of fruit salad samples (97%) were contaminated with yeasts at levels ranging from <2.0 to 9.72 log10 of colony forming units per gram ...200616943069
efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination.the presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. in citrus, selection using the selectable marker gene nptii, that confers resistance to the antibiotic kanamycin, is in general very effective. an attractive alternative is offered by the mat system (multi-auto-transformation), which combines the ipt gene for positive selection with the recombinase system r/rs for removal of ma ...200716927091
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