Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| an organic acid-tolerant haa1-overexpression mutant of an industrial bioethanol strain of saccharomyces cerevisiae and its application to the production of bioethanol from sugarcane molasses. | bacterial contamination is known as a major cause of the reduction in ethanol yield during bioethanol production by saccharomyces cerevisiae. acetate is an effective agent for the prevention of bacterial contamination, but it negatively affects the fermentation ability of s. cerevisiae. we have proposed that the combined use of organic acids including acetate and lactate and yeast strains tolerant to organic acids may be effective for the elimination of principally lactic acid bacterial (lab) co ... | 2013 | 24373204 |
| the fermentation of sugarcane molasses by dekkera bruxellensis and the mobilization of reserve carbohydrates. | the yeast dekkera bruxellensis is considered to be very well adapted to industrial environments, in brazil, usa, canada and european countries, when different substrates are used in alcoholic fermentations. our previous study described its fermentative profile with a sugarcane juice substrate. in this study, we have extended its physiological evaluation to fermentation situations by using sugarcane molasses as a substrate to replicate industrial working conditions. the results have confirmed the ... | 2014 | 24370978 |
| use of spectroscopic and imaging techniques to evaluate pretreated sugarcane bagasse as a substrate for cellulase production under solid-state fermentation. | the enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals. here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (scb) used for cellulase production was investigated by means of spectroscopic and imaging techniques. chemical composition and structural characteristics, such as crystallinity (determined by ... | 2014 | 24363237 |
| structure and function of a novel cellulase 5 from sugarcane soil metagenome. | cellulases play a key role in enzymatic routes for degradation of plant cell-wall polysaccharides into simple and economically-relevant sugars. however, their low performance on complex substrates and reduced stability under industrial conditions remain the main obstacle for the large-scale production of cellulose-derived products and biofuels. thus, in this study a novel cellulase with unusual catalytic properties from sugarcane soil metagenome (cele1) was isolated and characterized. the polype ... | 2013 | 24358302 |
| draft genome sequence of pseudozyma brasiliensis sp. nov. strain ghg001, a high producer of endo-1,4-xylanase isolated from an insect pest of sugarcane. | here, we present the nuclear and mitochondrial genome sequences of pseudozyma brasiliensis sp. nov. strain ghg001. p. brasiliensis sp. nov. is the closest relative of pseudozyma vetiver. p. brasiliensis sp. nov. is capable of growing on xylose or xylan as a sole carbon source and has great biotechnological potential. | 2013 | 24356824 |
| short and long-term antinutritional effect of the trypsin inhibitor apti for biological control of sugarcane borer. | plant-derived trypsin inhibitors have been shown to have potent anti-insect effects and are a promising alternative for the biological control of pests. in this work, we tested the anti-insect activity of adenanthera pavonina trypsin inhibitor (apti) against diatraea saccharalis larvae, a major insect pest in sugarcane. the addition of 0.1% apti in short-term assays resulted in 87% and 63% decreased trypsin and chymotrypsin activities respectively. apti was not digested after 60h incubation with ... | 2014 | 24355140 |
| a new species of burkholderia isolated from sugarcane roots promotes plant growth. | sugarcane is a globally important food, biofuel and biomaterials crop. high nitrogen (n) fertilizer rates aimed at increasing yield often result in environmental damage because of excess and inefficient application. inoculation with diazotrophic bacteria is an attractive option for reducing n fertilizer needs. however, the efficacy of bacterial inoculants is variable, and their effective formulation remains a knowledge frontier. here, we take a new approach to investigating diazotrophic bacteria ... | 2014 | 24350979 |
| a novel promising trichoderma harzianum strain for the production of a cellulolytic complex using sugarcane bagasse in natura. | brazil is a major producer of agro-industrial residues, such as sugarcane bagasse, which could be used as raw material for microbial production of cellulases as an important strategy for the development of sustainable processes of second generation ethanol production. for this purpose, this work aimed at screening for glycosyl hydrolase activities of fungal strains isolated from the brazilian cerrado. among 13 isolates, a trichoderma harzianum strain (l04) was identified as a promising candidate ... | 2013 | 24349958 |
| comparative genomic analysis of saccharomyces cerevisiae yeasts isolated from fermentations of traditional beverages unveils different adaptive strategies. | saccharomyces cerevisiae strains are the main responsible of most traditional alcohol fermentation processes performed around the world. the characteristics of the diverse traditional fermentations are very different according to their sugar composition, temperature, ph or nitrogen sources. during the adaptation of yeasts to these new environments provided by human activity, their different compositions likely imposed selective pressures that shaped the s. cerevisiae genome. in the present work ... | 2014 | 24334254 |
| field performance of transgenic sugarcane produced using agrobacterium and biolistics methods. | future genetic improvement of sugarcane depends, in part, on the ability to produce high-yielding transgenic cultivars with improved traits such as herbicide and insect resistance. here, transgenic sugarcane plants generated by different transformation methods were assessed for field performance over 3 years. agrobacterium-mediated (agro) transgenic events (35) were produced using four different agrobacterium tumefaciens strains, while biolistic (biol) transgenic events (48) were produced using ... | 2014 | 24330327 |
| a vigorous mutant sugarcane (saccharum sp.) clone co 527. | a vigorous fast growing mutant which ends vegetative growth sixty days earlier than the parent variety co 527 was isolated from gamma irradiated vegetative buds. the mutant initially segregated but stabilized in the vm4 generation. its growth rate was almost fifty per cent higher than co 527 beginning in the early stages of growth. it produced a significantly higher early shoot population which enabled it to yield a higher number of millable canes at maturity. economic characters like sucrose co ... | 1978 | 24317906 |
| a survey for isoenzymes of glucosephosphate isomerase, phosphoglucomutase, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in c3-, c 4-and crassulacean-acid-metabolism plants, and green algae. | two isoenzymes each of glucosephosphate isomerase (ec 5.3.1.9), phosphoglucomutase (ec 2.7.5.1), glucose-6-phosphate dehydrogenase (ec 1.1.1.49) and 6-phosphogluconate dehydrogenase (ec 1.1.1.43) were separated by (nh4)2so4 gradient solubilization and deae-cellulose ion-exchange chromatography from green leaves of the c3-plants spinach (spinacia oleracea l.), tobacco (nicotiana tabacum l.) and wheat (triticum aestivum l.), of the crassulacean-acid-metabolism plants crassula lycopodioides lam., b ... | 1979 | 24317570 |
| selection studies in sugarcane (saccharum sp. hybrids) : iii. a method to determine sample size for the estimation of population variance. | an approximate method to determine sample size for the estimation of population variance, σ(2), is given. the estimate of σ(2) is denoted as s(2) . based on the assumption of a normal distribution for (s(2)/σ(2)-1), the sample size is approximately equal to 20,000 z(2) p,/k(2); where z is a standard normal deviate, p is the probability that δs(2) (≡ 100¦s(2) - σ(2)¦/σ(2)) is less than, or equal to, a critical value k, and k (measured as gds(2)) is the desired precision of s(2) .the expected valu ... | 1977 | 24317535 |
| water stress reveals differential antioxidant responses of tolerant and non-tolerant sugarcane genotypes. | the biochemical responses of the enzymatic antioxidant system of a drought-tolerant cultivar (iacsp 94-2094) and a commercial cultivar in brazil (iacsp 95-5000) grown under two levels of soil water restriction (70% and 30% soil available water content) were investigated. iacsp 94-2094 exhibited one additional active superoxide dismutase (cu/zn-sod vi) isoenzyme in comparison to iacsp 95-5000, possibly contributing to the heightened response of iacsp 94-2094 to the induced stress. the total gluta ... | 2014 | 24308986 |
| combining ability and parental evaluation in five selected clones of sugarcane (saccharum sp. hybrids). | five hawaiian commercial sugarcane (saccharum sp. hybrids) clones were crossed in a full diallel. four morphological characters were studied in the progeny: number of millable stalks per plant, stalk diameter, stalk length, and sucrose content. a fifth character, plant volume, was calculated from stalk number, stalk diameter and stalk length. the five selected parental clones were treated as fixed variables in the analysis. general combining ability (gca) was significant for all five characters; ... | 1980 | 24305911 |
| brewer's yeast and sugarcane yeast as protein sources for dogs. | brewer's yeast (by), autolysed sugarcane yeast (ascy) and integral sugar cane yeast (iscy) were studied in two experiments as ingredients for dog diets. in the first experiment, 28 dogs were randomly assigned to four diets; one reference diet and three test diets containing 15% of by, ascy or iscy and 85% of the reference diet (as-fed basis). the digestibilities of the yeasts were calculated by the substitution method. in the second experiment, 35 dogs were randomized to five diets with similar ... | 2014 | 24304448 |
| analysis of mitochondrial dna from somatic hybrid cell lines of saccharum officinarum (sugarcane) and pennisetum americanum (pearl millet). | mitochondrial dna (mtdna) from cell suspension cultures of two intergeneric somatic hybrids of pennisetum americanum (pearl millet) + saccharum officinarum (sugarcane) was examined by restriction endonuclease digestion and hybridization with sorghum mtdna cosmids. the mtdna of one somatic hybrid was indistinguishable from that of pearl millet, while the second exhibited a combination of parental mtdnas, suggesting mitochondrial fusion. several novel, possibly recombinant, mtdna restriction fragm ... | 1987 | 24301313 |
| the determination of assay for laccase of bacillus subtilis wpi with two classes of chemical compounds as substrates. | ligninolytic enzyme complexes are involved in lignin degradation. among them laccases are outstanding because they use molecular oxygen as a co-substrate instead of hydrogen peroxide as used by peroxidases. bacterial laccase of bacillus genus was first reported in claus and filip (microbiol res 152:209-216, 1997), since then more bacterial laccases have been found. in this research, laccase-producing bacteria were screened from pulp and paper industry wastewater, bagass and sugarcane rhizosphere ... | 2012 | 24293734 |
| biohydrogen production from sugarcane bagasse by integrating dark- and photo-fermentation. | hydrogen production from sugarcane bagasse (scb) by integrating dark-fermentation by enterobacter aerogenes mtcc 2822 and photo-fermentation by rhodopseudomonas bhu 01 was investigated. the scb was hydrolysed by sulphuric acid and the hydrolysate detoxified by passing through adsorbent resin column (amberlite xad-4) to remove the inhibitory furfural, and subjected to dark-fermentation. the cellulosic residue from acid hydrolysis was hydrolysed by the new isolate cellulomonas fimi to release suga ... | 2014 | 24291314 |
| exploring grape marc as trove for new thermotolerant and inhibitor-tolerant saccharomyces cerevisiae strains for second-generation bioethanol production. | robust yeasts with high inhibitor, temperature, and osmotic tolerance remain a crucial requirement for the sustainable production of lignocellulosic bioethanol. these stress factors are known to severely hinder culture growth and fermentation performance. | 2013 | 24286305 |
| fungal pretreatment improves amenability of lignocellulosic material for its saccharification to sugars. | the sugarcane bagasse was biologically pretreated with three white-rot fungi; pleurotus florida, coriolopsis caperata rck 2011 and ganoderma sp. rckk-02, individually under solid-state fermentation. p. florida, c. caperata rck 2011 and ganoderma sp. rckk-02 degraded lignin up to 7.91, 5.48 and 5.58%, respectively. the lignocellulolytic enzymes produced by these fungi were also monitored during solid state fermentation of sugarcane bagasse. the fungal fermented sugarcane bagasse when hydrolyzed w ... | 2014 | 24274505 |
| photoautotrophic production of d-lactic acid in an engineered cyanobacterium. | the world faces the challenge to develop sustainable technologies to replace thousands of products that have been generated from fossil fuels. microbial cell factories serve as promising alternatives for the production of diverse commodity chemicals and biofuels from renewable resources. for example, polylactic acid (pla) with its biodegradable properties is a sustainable, environmentally friendly alternative to polyethylene. at present, pla microbial production is mainly dependent on food crops ... | 2013 | 24274114 |
| optimization, kinetics, and modeling of inulinase production by k. marxianus var. marxianus. | pressmud, a by-product from the sugarcane industry, was used as a carbon source for the production of inulinase in solid-state fermentation (ssf). statistical experimental designs were employed to screen the nutrients and optimize the media composition for the production of inulinase by kluyveromyces marxianus var. marxianus. eighteen various nutrients were selected for preliminary screening of production medium component by plackett-burman design (pbd) technique. five nutrients were found to be ... | 2014 | 24274017 |
| leaf vasculature in sugarcane (saccharum officinarum l.). | the vascular system of the leaves of saccharum officinarum l. is composed in part of a system of longitudinal strands that in any given transverse section may be divided into three types of bundle according to size and structure: small, intermediate, and large. virtually all of the longitudinal strands intergrade, however, from one type bundle to another. for example, virutually all of the strands having large bundle anatomy appear distally in the blade as small bundles, which intergrade into in ... | 1982 | 24272309 |
| in vitro propagation of a saccharum officinarum (l.) and sclerostachya fusca (roxb.) a. camus hybrid. | callus induction and plant differentiation were obtained in an intergeneric hybrid of saccharum officinarum and sclerostachya fusca. the sub clones showed morphological variation. chromosome numerical variation was not observed but structural aberrations were noticed in some sub clones. the study indicates the use of tissue culture technique for inducing intergeneric gene transfer in saccharum hybrids. | 1984 | 24258543 |
| plant regeneration via somatic embryogenesis in sugarcane. | plants, regenerated from callus cultures of sugarcane (saccharum officinarum l.) clone ij76-316, originated through somatic embryogenesis. callus cultures were established from primordial leaves and apical meristems on murashige and skoog medium (ms) supplemented with 3 mg 1(-1) 2,4-dichlorophenoxy acetic acid and 100 ml 1(-1) coconut water (msc3). nodular calli formed within 2 weeks of culture. calli were maintained on msc3 medium by transfer every 3 to 4 weeks. somatic embryogenesis occurred a ... | 1983 | 24257849 |
| transient expression of electroporated dna in monocotyledonous and dicotyledonous species. | transient expression of electroporated dna was monitored in protoplasts of several monocot and dicot species by assaying for expression of chimeric chloramphenicol acetyltransferase (cat) gene constructions. expression was obtained in the dicot species of daucus carota, glycine max and petunia hybrida and the monocot species of triticum monococcum, pennisetum purpureum, panicum maximum, saccharum officinarum, and a double cross, trispecific hybrid between pennisetum purpureum, p. americanum, and ... | 1987 | 24248756 |
| constituents of wing gland and abdominal hair pencil secretions of male african sugarcane borer,eldana saccharina walker (lepidoptera: pyralidae). | in addition totrans-3,7-dimethyl-6-octen-4-olide (eldanolide), vanillin, and 4-hydroxybenzaldehyde, identified by french workers in the wing gland and abdominal hair pencil secretions of the male african sugarcane borer,eldana saccharina, we have, in an earlier note, reported the presence of several other terpenoid, aromatic, and unbranched-chain compounds such as, (z)-3,7-dimethylocta-2,6-dienoic acid, 6,10,14-trimethyl-2-pentadecanol, 4-hydroxy-3-methoxybenzyl alcohol, 1-octadecane thiol, 16-h ... | 1993 | 24248574 |
| evaluation of cultivar-testing locations in sugarcane. | selection of test locations, representative of conditions and practices of an area can be a challenging process in a breeding program. data from two groups of sugarcane (trispecies hybrids of saccharum sp.) cultivar experiments in florida were analyzed to determine if relative cultivar response at any two of six current locations was sufficiently similar so that at least one location could be replaced by a location with a different environment. the parameter analyzed was metric tons per ha of su ... | 1985 | 24247333 |
| evidence for the involvement of a udp-glucose-dependent group translocator in sucrose uptake into vacuoles of storage roots of red beet. | vacuoles isolated from the storage roots of red beet (beta vulgaris l.) accumulate sucrose via two different mechanisms. one mechanism transports sucrose directly, and its rate is increased by the addition of mgatp. the other mechanism utilizes uridine diphosphate glucose (udp-glucose) to synthesize and simultaneously transport sucrose phosphate and sucrose into the vacuole. this group translocation mechanism has also been found in sugarcane vacuoles. as in sugarcane, the beet group translocator ... | 1986 | 24240312 |
| the oxidation of extracellular nadh by sugarcane cells: coupling to ferricyanide reduction, oxygen uptake and ph change. | suspension-cultured cells of sugarcane (saccharum sp. hybrids) did not oxidize exogenously supplied nadh in the absence of ferricyanide (potassium hexacyanoferrate [iii]), whereas they did at a low rate in the presence of ferricyanide. concomitantly, ferricyanide was reduced at a slow rate. neither a ph change nor a change in respiration was caused by the addition of nadh and-or ferricyanide, but ferricyanide was a strong inhibitor of sugar transport. in contrast to cells, protoplasts rapidly ox ... | 1987 | 24232839 |
| effect of several environmental parameters on carbon metabolism in histosols. | high specific activity(14)c-labeled glucose, succinate, acetate, salicylate, and amino acids were used to examine carbon metabolism by the microbial community of pahokee muck (alithic medisaprist), a drained, cultivated soil of the florida everglades. variations in carbon oxidation were observed from the end of the wet season through the dry season in a fallow (bare) field. evolution of(14)co2 varied with the substrate added and time. calculation of(14)co2 evolution for each substrate as a propo ... | 1980 | 24232520 |
| evidence for a uridine-5'-diphosphate-glucose-protectedp-chloromercuribenzene sulfonic acid-binding site in sugarcane vacuoles. | the uptake of uridine-5'-diphosphate (udp) glucose into vacuoles isolated fromsaccharum sp. cells was fully inhibited by pretreatment with 50 μmp-chloromercuribenzenesulfonic acid (pcmbs) and was not affected by n-ethylmaleimide up to a concentration of 5 mm. the addition of 10 mm udp-glucose during the pretreatment partially protected the uptake mechanism from pcmbs inhibition, while the presence of adenosine-5'-diphosphate (adp) glucose or of various hexose-phosphates had no protective effect. ... | 1986 | 24232430 |
| plant regeneration from a cryopreserved embryogenic cell suspension of a commercial sugarcane hybrid (saccharum sp.). | efficient plant regeneration was obtained from a cryopreserved embryogenic cell suspension of sugarcane established from leaf derived callus. pregrowing the cells for three days in ms basal medium supplemented with 0.33 m sorbitol was essential to the process. the cells were cooled at a rate of 0.5°c/min to -40°c and then stored in liquid nitrogen. thawing was carried out rapidly in water at +40°c, and the cells were then plated without washing onto filter paper discs placed on a semi-solid rege ... | 1990 | 24227168 |
| protein polymorphism in sugarcane revealed by two-dimensional gel analysis. | in order to identify molecular markers for the analysis of the sugarcane genome, proteins extracted from apical segments of shoot tissues were resolved by a combination of equilibrium (ief) and nonequilibrium (nephge) two-dimensional polyacrylamide gel electrophoresis. a number of taxa of the "saccharum complex" group (saccharum species and the related genera of andropogoneae) with presumed contributions to the sugarcane genome were surveyed. protein profiles were compared to a reference map con ... | 1990 | 24226346 |
| uridine 5'-diphospho-d-glucose-dependent vectorial sucrose synthesis in tonoplast vesicles of the storage hypocotyl of red beet (beta vulgaris l. ssp. conditiva). | tonoplast vesicles were prepared from red-beet (beta vulgaris l. ssp. conditiva) hypocotyl tubers ("beetroot") known to store sucrose. uptake experiments, employing uridine 5'-diphospho-[(14)c]glucose (udp-[(14)c]glucose) showed the operation of an udp-glucose-dependent group translocator for vectorial synthesis and accumulation of sucrose, recently described for sugarcane and red-beet vacuoles and for tonoplast vesicles prepared from sugarcane suspension cells. characterization of the kinetic p ... | 1988 | 24226185 |
| design and optimization of a process for sugarcane molasses fermentation by saccharomyces cerevisiae using response surface methodology. | a statistical model was developed in this study to describe bioethanol production through a batch fermentation process of sugarcane molasses by locally isolated saccharomyces cerevisiae y-39. response surface methodology rsm based on central composite face centered design ccfd was employed to statistically evaluate and optimize the conditions for maximum bioethanol production and study the significance and interaction of incubation period, initial ph, incubation temperature, and molasses concent ... | 2013 | 24222769 |
| culture-independent analysis of endophytic bacterial communities associated with brazilian sugarcane. | sugarcane is an economically important culture in brazil. endophytic bacteria live inside plants, and can provide many benefits to the plant host. we analyzed the bacterial diversity of sugarcane cultivar rb-72454 by cultivation-independent techniques. total dna from sugarcane stems from a commercial plantation located in paraná state was extracted. partial 16s rrna genes were amplified and sequenced for library construction. of 152 sequences obtained, 52% were similar to 16s rrna from pseudomon ... | 2013 | 24222230 |
| lindane degradation by candida vitjzn04, a newly isolated yeast strain from contaminated soil: kinetic study, enzyme analysis and biodegradation pathway. | a new yeast strain was isolated from sugarcane cultivation field which was able to utilize lindane as sole carbon source for growth in mineral medium. the yeast was identified and named as candida sp. vitjzn04 based on a polyphasic approach using morphological, biochemical and 18s rdna, d1/d2 and its sequence analysis. the isolated yeast strain efficiently degraded 600 mg l⁻¹ of lindane within 6 days in mineral medium under the optimal conditions (ph 7; temperature 30 °c and inoculum dosage 0.06 ... | 2014 | 24217897 |
| effects of sugarcane waste-products on cd and zn fractionation and their uptake by sugarcane (saccharum officinarum l.). | the effects of three sugarcane waste-products from an ethanol production plant on the fractionation of cd and zn in high cd and zn contaminated soil and metal accumulation in sugarcane (saccharum officinarum l.) were studied, using the bcr sequential extraction and aqua regia extraction procedures. a pot experiment was performed for 4 months with four treatments: no-amendments (control), boiler ash (3% w/w), filter cake (3% w/w) and a combination of boiler ash and vinasse (1.5% + 1.5%, w/w). the ... | 2014 | 24217524 |
| phenolic profile, antioxidant potential and dna damage protecting activity of sugarcane (saccharum officinarum). | the present study investigated the antioxidant and phenolic composition of sugarcane. the leaves and juices of thirteen varieties of sugarcane were studied for their antioxidant activity and protective effect on dna damage. 2,2-diphenyl-1-picrylhydrazyl radical (dpph) assay was used to determine the radical scavenging activities in leaves and juices. different varieties of sugarcane showed good antioxidant properties, ic50 values ranged from 20.82 to 27.47 μg/ml for leaves and from 63.95 to high ... | 2014 | 24206679 |
| effects of tissue type and promoter strength on transient gus expression in sugarcane following particle bombardment. | effects of tissue type and promoter strength on transient gus expression in the sugarcane (saccharum spp. hybrids) cultivar nco 310 were evaluated following microprojectile bombardment of leaf explants. gus expression was histochemically or fluorometrically measured 48 h after delivery of the uida gene. high levels of gus expression were obtained in leaf segments isolated from young, expanding sugarcane leaves cultured for 1, 3, or 6 d prior to bombardment. the promoter derived from the maize ub ... | 1993 | 24201961 |
| complete genome sequence of leifsonia xyli subsp. cynodontis strain dsm46306, a gram-positive bacterial pathogen of grasses. | we announce the complete genome sequence of leifsonia xyli subsp. cynodontis, a vascular pathogen of bermuda grass. the species also comprises leifsonia xyli subsp. xyli, a sugarcane pathogen. since these two subspecies have genome sequences available, a comparative analysis will contribute to our understanding of the differences in their biology and host specificity. | 2013 | 24201198 |
| homo- and heterofermentative lactobacilli differently affect sugarcane-based fuel ethanol fermentation. | bacterial contamination during industrial yeast fermentation has serious economic consequences for fuel ethanol producers. in addition to deviating carbon away from ethanol formation, bacterial cells and their metabolites often have a detrimental effect on yeast fermentative performance. the bacterial contaminants are commonly lactic acid bacteria (lab), comprising both homo- and heterofermentative strains. we have studied the effects of these two different types of bacteria upon yeast fermentat ... | 2014 | 24198118 |
| cryopreservation of apices of in vitro plantlets of sugarcane (saccharum sp. hybrids) using encapsulation/dehydration. | a cryopreservation process using encapsulation/dehydration was set up for apices sampled on in vitro plantlets of sugarcane. after dissection, apices were cultured for one day on standard medium and then encapsulated in medium with 3% alginate. optimal conditions comprised preculture for 2 days in liquid medium with 250 g.l(-1) sucrose, desiccation for 6 hours under the laminar flow or for 10-11 hours with silicagel followed by rapid freezing and slow thawing. survival after freezing in liquid n ... | 1993 | 24196115 |
| enhanced gus gene expression in cereal/grass cell suspensions and immature embryos using the maize uhiquitin-based plasmid pahc25. | transient gus (β-glucuronidase) expression was visualized in cell suspensions of triticum aestivum, zea mays, pennisetum glaucum, saccharum officinarum, pennisetum purpureum and panicum maximum after microprojectile bombardment with pbargus and pahc25 plasmid dnas. pbargus contains the gus (uida) gene coding region driven by the adh1 promoter and the adh1 intron 1, as well as the bar gene coding region driven by the camv 35s promoter and the adh1 intron 1. pahc25 contains the gus and bar gene co ... | 1993 | 24196107 |
| sucrose storage in cell suspension cultures of saccharum sp. (sugarcane) is regulated by a cycle of synthesis and degradation. | we have investigated the regulation of sucrose storage in cell-suspension cultures of sugarcane. when grown in batch culture, sucrose accumulation commences after about 5 d, when the nitrogen supply is exhausted. sucrose storage is also induced by decreasing the nitrogen supply to cells growing in a chemostat. the measured activity of sucrose-phosphate synthase is high enough to account for the rate of sucrose accumulation, provided precautions are taken to avoid the hydrolysis of udp during the ... | 1991 | 24193530 |
| protein variation accompanies leaf dedifferentiation in sugarcane (sacchamm officinarum) and is influenced by genotype. | tissue culture lines (morphogenic and nonmorphogenic) were established in five genotypes of saccharum officinarum l. changes in protein expression after dedifferentiation of leaf tissue into callus were investigated by two-dimensional gel electrophoresis of cellular proteins. the findings demonstrated that protein expression was regulated both quantitatively and qualitatively in all five genotypes of sugarcane. sixty-three dedifferention-proteins were identified, of which thirty-three were commo ... | 1994 | 24193522 |
| phylogenetic analysis of chloroplast restriction enzyme site mutations in the saccharinae griseb. subtribe of the andropogoneae dumort. tribe. | chloroplast (cp) dna from 32 genotypes representing eight genera and 19 species from the andropogoneae tribe was analyzed using 15 restriction enzymes and southern hybridization with 12 cpdna probes that span the complete rice chloroplast genome. six of the genera, saccharum, miscanthus, erianthus, narenga, eccoilopus, and sclerostachya, are part of the saccharinae subtribe, whereas the other two, zea and sorghum, were used as outgroups. narenga, miscanthus, erianthus, and sclerostachya are pres ... | 1994 | 24190471 |
| potential of sugarcane bagasse (agro-industrial waste) for the production of bacillus thuringiensis israelensis. | sugarcane bagasse is a renewable resource that can be used to produce biopesticide for the control of mosquito vectors. in the present study, we demonstrated that cane processed bagasse could be used to produce bacillus thuringiensis serovar israelensis (bti) for control of mosquito vectors viz: culex quinquefasciatus, anopheles stephensi and aedes aegypti. biochemical studies indicated that the bti spore/crystal toxins produced from the test culture medium (bagasse, bg + soybean, sb) are higher ... | 2013 | 24189680 |
| production of transgenic sugarcane (saccharum officinarum l.) plants by intact cell electroporation. | we describe an efficient procedure for genetic transformation of commercial sugarcane varieties poj 2878 and ja 60-5. the transformation protocol is based on electroporation of a plasmid conferring gus activity into cell clusters isolated from embryogenic calli. six to eight weeks after electroporation, ja 60-5 plants regenerated from electroporated tissues were tested and confirmed to be transgenic using histochemical glucuronidase and southern hybridization analysis. electroporation of intact ... | 1995 | 24186765 |
| solute distribution between vacuole and cytosol of sugarcane suspension cells: sucrose is not accumulated in the vacuole. | the compartmentation of solutes in suspension cells of saccharum sp. during different growth phases in batch culture was determined using cucl2 to permeabilize the plasma membrane of the cells. the efflux of cytosolic and vacuolar pools of sugars, cations and phosphate was monitored, and the efflux data for phosphate were compared and corrected using data from compartmentation analysis of phosphate as determined by (31)p-nuclear magnetic resonance spectroscopy. the results show that sucrose is n ... | 1992 | 24186659 |
| sucrose uptake into vacuoles of sugarcane suspension cells. | uptake of sucrose into vacuoles of suspension cells of saccharum sp. (sugarcane) was investigated using a vacuole-isolation method based on osmotic- and ph-dependent lysis of protoplasts. vacuoles took up sucrose at high rates without the influence of tonoplast energization on sucrose transport. neither addition of atp or pyrophosphate nor dissipation of the membrane potential or the ph gradient by ionophores changed uptake rates appreciably. generation of an atp-dependent ph gradient across the ... | 1991 | 24186582 |
| phylogenetic analysis of organellar dna sequences in the andropogoneae: saccharinae. | to study the phylogenetics of sugarcane (saccharum officinarum l.) and its relatives we sequenced four loci on cytoplasmic genomes (two chloroplast and two mitochondrial) and analyzed mitochondrial rflps generated using probes for coxi, coxii, coxiii, cob, 18s+5s, 26s, atpase 6, atpase 9, and atpase α (d'hont et al. 1993). approximately 650 bp of dna in the intergenic spacer region between rbcl and atpb and approximately 150 bp from the chloroplast 16s rdna through the intergenic spacer region t ... | 1994 | 24186245 |
| superoxide dismutase and ascorbate peroxidase improve the recovery of photosynthesis in sugarcane plants subjected to water deficit and low substrate temperature. | the physiological responses of c4 species to simultaneous water deficit and low substrate temperature are poorly understood, as well as the recovery capacity. this study investigated whether the effect of these abiotic stressors is cultivar-dependent. the differential responses of drought-resistant (iacsp94-2094) and drought-sensitive (iacsp97-7065) sugarcane cultivars were characterized to assess the relationship between photosynthesis and antioxidant protection by apx and sod isoforms under st ... | 2013 | 24184453 |
| molecular variability of sugarcane streak mosaic virus in china based on an analysis of the p1 and cp protein coding regions. | sequences of the protein 1 (p1) and coat protein (cp) coding regions of 22 sugarcane streak mosaic virus (scsmv) isolates were determined. phylogenetic analysis showed that scsmv had at least three major lineages, and the lineages seemed to reflect geographical origin. the sudden expansions of the chinese and indian subpopulations were supported by calculations showing deviations from the neutral equilibrium model for the individual lineages with an overall lack of nucleotide diversity. our stud ... | 2014 | 24178307 |
| chromosome assortment in saccharum. | recent work has revealed random chromosome pairing and assortment in saccharum spontaneum l., the most widely distributed, and morphologically and cytologically variable of the species of saccharum. this conclusion was based on the analysis of a segregating population from across between s. spontaneum 'ses 208' and a spontaneously-doubled haploid of itself, derived from anther culture. to determine whether polysomic inheritance is common in saccharum and whether it is observed in a typical bipar ... | 1994 | 24178110 |
| the capability of endophytic fungi for production of hemicellulases and related enzymes. | there is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. one of the most successful options is ethanol obtained mainly from sugarcane and corn fermentation. the foremost residue from sugarcane industry is the bagasse, a rich lignocellulosic raw material uses for the production of ethanol second generation (2g). new cellulolytic and hemicellulytic enzymes are needed, in order to optimize the degradation of bagasse and production of etha ... | 2013 | 24175970 |
| optimization of endoglucanase and xylanase activities from fusarium verticillioides for simultaneous saccharification and fermentation of sugarcane bagasse. | enzymatic hydrolysis is an important but expensive step in the production of ethanol from biomass. thus, the production of efficient enzymatic cocktails is of great interest for this biotechnological application. the production of endoglucanase and xylanase activites from f. verticillioides were optimized in a factorial design (2(5)) followed by a ccdr design. endoglucanase and xylanase activities increased from 2.8 to 8.0 u/ml and from 13.4 to 114 u/ml, respectively. the optimal ph and temperat ... | 2014 | 24170331 |
| identification and characterisation of sugarcane intergeneric hybrids, saccharum officinarum x erianthus arundinaceus, with molecular markers and dna in situ hybridisation. | molecular markers were used to characterise sugarcane intergeneric hybrids between s. officinarum and e. arundinaceus. very simple diagnostic tools for hybrid identification among the progeny were derived from isozyme electrophoresis and a sequence-tagged pcr. two enzyme systems (got and mdh b) and pcr amplification revealing spacer-size variation in the 5s-rdna cluster were found most convenient. specific characterisation of the two genomic components was possible using rflp and in situ hybridi ... | 1995 | 24169780 |
| continuous ethanol production from sugarcane molasses using a newly designed combined bioreactor system by immobilized saccharomyces cerevisiae. | continuous ethanol fermentation using polyvinyl alcohol (pva), immobilized yeast, and sugarcane molasses (22 and 35°bx) with 8 g/l urea was run in a combined bioreactor system consisting of three-stage tubular bioreactors in series. the effect of the dilution rate (d) at 0.0037, 0.0075, 0.0117, 0.0145, 0.018, and 0.0282 h(-1) on continuous ethanol fermentation was investigated in this study. the results showed that d had a significant effect on fermentation efficiency, sugar-utilized rate, ethan ... | 2014 | 24164318 |
| agricultural case studies of classification accuracy, spectral resolution, and model over-fitting. | this paper describes the relationship between spectral resolution and classification accuracy in analyses of hyperspectral imaging data acquired from crop leaves. the main scope is to discuss and reduce the risk of model over-fitting. over-fitting of a classification model occurs when too many and/or irrelevant model terms are included (i.e., a large number of spectral bands), and it may lead to low robustness/repeatability when the classification model is applied to independent validation data. ... | 2013 | 24160886 |
| identification and validation of sugarcane streak mosaic virus-encoded micrornas and their targets in sugarcane. | plants have developed several defense mechanisms to cope with various pathogens (bacteria, fungi, virus, and phytoplasma). among these, rna interference (rnai)-mediated defense against viral infection was found to be a major innate immune response. as a counter attack strategy against the host defense, viruses produce suppressors of host rnai pathway. micrornas (mirnas) are an abundant class of short (~18-22 nucleotide) non-coding single-stranded rnas involved in rnai pathway leading to post-tra ... | 2014 | 24145912 |
| centesimal composition and physical-chemistry analysis of the edible mushroom lentinus strigosus occurring in the brazilian amazon. | the centesimal composition and the physical and chemical analyses of lentinus strigosus, an edible mushroom occurring in the brazilian amazon and produced in alternative substrates based on wood and agroindustrial residues, were evaluated. for this purpose, the c, n, ph, soluble solids, water activity, protein, lipids, total fiber, ash, carbohydrate, and energy levels were determined. the substrates were formulated from simarouba amara aubl. ("marupá"), ochroma piramidale cav. ex. lam. ("pau-de- ... | 2013 | 24141410 |
| combined linkage and association mapping reveals candidates for scmv1, a major locus involved in resistance to sugarcane mosaic virus (scmv) in maize. | sugarcane mosaic virus (scmv) disease causes substantial losses of grain yield and forage biomass in susceptible maize cultivars. maize resistance to scmv is associated with two dominant genes, scmv1 and scmv2, which are located on the short arm of chromosome 6 and near the centromere region of chromosome 3, respectively. we combined both linkage and association mapping to identify positional candidate genes for scmv1. | 2013 | 24134222 |
| cloning, expression and characterization of sugarcane (saccharum officinarum l.) transketolase. | pentose phosphate pathway (ppp) composed of two functionally-connected phases, the oxidative and non-oxidative phase. both phases catalysed by a series of enzymes. transketolase is one of key enzymes of non-oxidative phase in which transfer two carbon units from fructose-6-phosphate to erythrose-4-phosphate and convert glyceraldehyde-3-phosphate to xylulose-5-phosphate. in plant, erythrose-4-phosphate enters the shikimate pathway which is produces many secondary metabolites such as aromatic amin ... | 2013 | 24132392 |
| saccharification of biomass using whole solid-state fermentation medium to avoid additional separation steps. | the enzymatic hydrolysis of steam-exploded sugarcane bagasse (sesb) was investigated using enzymatic extracts (ee) and whole fermentation media (wm), produced in-house, from aspergillus niger 3t5b8 and trichoderma reesei rut-c30 cultivated on wheat bran under solid-state fermentation (ssf). a detailed and quantitative comparison of the different hydrolysis conditions tested was carried out using the chrastil approach for modeling enzymatic reactions by fitting the experimental data of total redu ... | 2013 | 24115639 |
| emissions generated by sugarcane burning promote genotoxicity in rural workers: a case study in barretos, brazil. | to determine the possible genotoxic effect of exposure to the smoke generated by biomass burning on workers involved in manual sugar cane harvesting. | 2013 | 24112819 |
| efficient succinic acid production from lignocellulosic biomass by simultaneous utilization of glucose and xylose in engineered escherichia coli. | to enhance succinic acid formation during xylose fermentation in escherichia coli, overexpression of atp-forming phosphoenolpyruvate carboxykinase (pepck) from bacillus subtilis 168 in an ldha, pflb, and ppc deletion strain resulted in a significant increase in cell mass and succinic acid production. however, ba204 displays a low yield of glucose fermentation and sequential glucose-xylose utilization under regulation by the phosphotransferase system (pts). to improve the capability of glucose fe ... | 2013 | 24096277 |
| bioelectricity versus bioethanol from sugarcane bagasse: is it worth being flexible? | sugarcane is the most efficient crop for production of (1g) ethanol. additionally, sugarcane bagasse can be used to produce (2g) ethanol. however, the manufacture of 2g ethanol in large scale is not a consolidated process yet. thus, a detailed economic analysis, based on consistent simulations of the process, is worthwhile. moreover, both ethanol and electric energy markets have been extremely volatile in brazil, which suggests that a flexible biorefinery, able to switch between 2g ethanol and e ... | 2013 | 24088415 |
| sugarcane vinasse: environmental implications of its use. | the inadequate and indiscriminate disposal of sugarcane vinasse in soils and water bodies has received much attention since decades ago, due to environmental problems associated to this practice. vinasse is the final by-product of the biomass distillation, mainly for the production of ethanol, from sugar crops (beet and sugarcane), starch crops (corn, wheat, rice, and cassava), or cellulosic material (harvesting crop residues, sugarcane bagasse, and wood). because of the large quantities of vina ... | 2013 | 24084103 |
| agro-ecosystems impact malaria prevalence: large-scale irrigation drives vector population in western ethiopia. | development strategies in ethiopia have largely focused on the expansion of irrigated agriculture in the last decade to reduce poverty and promote economic growth. however, such irrigation schemes can worsen the socio-economic state by aggravating the problem of mosquito-borne diseases. in this study, the effect of agro-ecosystem practices on malaria prevalence and the risk of malaria transmission by the primary vector mosquito, anopheles arabiensis, in ethiopia were investigated. | 2013 | 24083353 |
| engineering and adaptive evolution of escherichia coli w for l-lactic acid fermentation from molasses and corn steep liquor without additional nutrients. | the d-lactic acid producing strain, escherichia coli hbut-d, was reengineered for l(+)-lactic acid fermentation by replacing the d-lactate dehydrogenase gene (ldha) with an l(+)-lactate dehydrogenase gene (ldhl) from pedicoccus acidilactici, followed by adaptive evolution in sucrose. the resulting strain, wyz-l, has enhanced expression of the sucrose operon (csca and csckb). in 100 g l(-1) of sucrose fermentation using mineral salt medium, wyz-l produced 97 g l(-1) of l(+)-lactic acid, with a yi ... | 2013 | 24063823 |
| occurrence of fungal species and mycotoxins from decayed sugarcane (saccharrum officinarum) in egypt. | seventy-three fungal species belonging to forty-three genera were isolated from 40 samples of saccharrum officinarum (collected from naage-hamadi canal in qena governorate, egypt). aspergillus, trichoderma, mucor and pythium were the most common genera on the two isolation media. the dominant species of aspergillus were a. niger, a. flavus, a. ustus, a. terreus and a. wentii. some species were dominant on 40 g/l sucrose such as aspergillus niger, a. flavus, emericella nidulans, trichoderma virid ... | 2005 | 24049478 |
| isolation and characterization of novel phorate-degrading bacterial species from agricultural soil. | based upon 16s rdna sequence homology, 15 phorate-degrading bacteria isolated from sugarcane field soils by selective enrichment were identified to be different species of bacillus, pseudomonas, brevibacterium, and staphylococcus. relative phorate degradation in a mineral salt medium containing phorate (50 μg ml(-1)) as sole carbon source established that all the bacterial species could actively degrade more than 97 % phorate during 21 days. three of these species viz. bacillus aerophilus strain ... | 2014 | 24046230 |
| effect of acid hydrolysis and fungal biotreatment on agro-industrial wastes for obtainment of free sugars for bioethanol production. | this study was designed to evaluate selected chemical and microbiological treatments for the conversion of certain local agro-industrial wastes (rice straw, corn stalks, sawdust, sugar beet waste and sugarcane bagasse) to ethanol. the chemical composition of these feedstocks was determined. conversion of wastes to free sugars by acid hydrolysis varied from one treatment to another. in single-stage dilute acid hydrolysis, increasing acid concentration from 1 % (v/v) to 5 % (v/v) decreased the con ... | 2012 | 24031984 |
| ethanol fermentation of sugarcane molasses by zymomonas mobilis mtcc 92 immobilized in luffa cylindrica l. sponge discs and ca-alginate matrices. | bio-ethanol production from cane molasses (diluted to 15 % sugar w/v) was studied using the bacterium, zymomonas mobilis mtcc 92 entrapped in luffa (luffa cylindrica l.) sponge discs and ca-alginate gel beads as the immobilizing matrices. at the end of 96 h fermentation, the final ethanol concentrations were 58.7 ± 0.09 and 59.1 ± 0.08 g/l molasses with luffa and ca-alginate entrapped z. mobilis cells, respectively exhibiting 83.25 ± 0.03 and 84.6 ± 0.02 % sugar conversion. there was no statisti ... | 2012 | 24031981 |
| selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation. | twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45°c on medium containing corn straw and cardboard as carbon sources. these fungi were identified in the genera aspergillus, thermomyces, myceliophthora, thermomucor and candida. the majority of the isolated strains produced xylanase and cellulases under solid state fermentation (ssf). the highest cellulase and xylanase productions wer ... | 2012 | 24031929 |
| specific plant induced biofilm formation in methylobacterium species. | two endophytic strains of methylobacterium spp. were used to evaluate biofilm formation on sugarcane roots and on inert wooden sticks. results show that biofilm formation is variable and that plant surface and possibly root exudates have a role in methylobacterium spp. host recognition, biofilm formation and successful colonization as endophytes. | 2011 | 24031703 |
| quantification of natural populations of gluconacetobacter diazotrophicus and herbaspirillum spp. in sugar cane (saccharum spp.) using differente polyclonal antibodies. | the species gluconacetobacter diazotrophicus, herbaspirillum seropedicae and h. rubrisubalbicans are endophytic n2-fixing [diazotrophic] bacteria which colonise not only roots, but also the aerial tissue of sugar cane. however, the technique most commonly used to quantify the populations of these microbes in plants is by culturing serial dilutions of macerates of plant tissues in n free semi-solid media which are only semi-selective for the species/genera [the most probable number (mpn) techniqu ... | 2009 | 24031435 |
| polyphasic characterization of gluconacetobacter diazotrophicus isolates obtained from different sugarcane varieties. | a polyphasic approach was applied to characterize 35 g. diazotrophicus isolates obtained from sugarcane varieties cultivated in brazil. the isolates were analyzed by phenotypic (use of different carbon sources) and genotypic tests (ardra and risa-rflp techniques). variability among the isolates was observed in relation to the carbon source use preference. glucose and sucrose were used by all isolates in contrast to myo-inositol, galactose and ribose that were not metabolized. the results of the ... | 2008 | 24031296 |
| molecular characterization of nitrogen-fixing bacteria isolated from brazilian agricultural plants at são paulo state. | fourteen strains of nitrogen-fixing bacteria were isolated from different agricultural plant species, including cassava, maize and sugarcane, using nitrogen-deprived selective isolation conditions. ability to fix nitrogen was verified by the acetylene reduction assay. all potentially nitrogen-fixing strains tested showed positive hybridization signals with a nifh probe derived from azospirillum brasilense. the strains were characterized by rapd, ardra and 16s rdna sequence analysis. rapd analyse ... | 2008 | 24031239 |
| dynamic distribution of potassium in sugarcane. | in this work the distribution of potassium in sugarcane has been studied during its growth. the soil was prepared with natural fertilizers prepared with sugarcane bagasse. for the measurement of potassium concentration in each part of the plant, gamma-ray spectrometry was used to measure gamma-rays emitted from the radioisotope (40)k. the concentrations of potassium in roots, stems and leaves were measured every two to three months beginning about five months after planting the sugarcane. the re ... | 2013 | 24016872 |
| phenolic content, antioxidant and antibacterial activity of selected natural sweeteners available on the polish market. | seventeen natural sweeteners available on the polish market were screened for total phenolic content, by the folin-ciocalteu method, and for antioxidant activity, using the ferric reducing antioxidant power (frap) assay and the 2,2'-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) radical cation decolorization assay (abts(·+)). in addition, we analyzed antibacterial activities against staphylococcus aureus strains: both those susceptible and those resistant to methicillin (mrsa). the results of ... | 2013 | 24007486 |
| metabolic engineering of saccharomyces cerevisiae for increased bioconversion of lignocellulose to ethanol. | the absence of pentose-utilizing enzymes in saccharomyces cerevisiae is an obstacle for efficiently converting lignocellulosic materials to ethanol. in the present study, the genes coding xylose reductase (xyl1) and xylitol dehydrogenase (xyl2) from pichia stipitis were successfully engineered into s. cerevisae. as compared to the control transformant, engineering of xyl1 and xyl2 into yeasts significantly increased the microbial biomass (8.1 vs. 3.4 g/l), xylose consumption rate (0.15 vs. 0.02 ... | 2012 | 23997337 |
| effect of unconventional carbon sources on biosurfactant production and its application in bioremediation. | the potential of an alkaliphilic bacterium klebsiella sp. strain rj-03, to utilize different unconventional carbon sources for the production of biosurfactant was evaluated. the biosurfactant produced using corn powder, potato peel powder, madhuca indica and sugarcane bagasse containing medium, exhibited significantly higher viscosity and maximum reduction in surface tension as compared to other substrates. among several carbon substrates tested, production of biosurfactant was found to be the h ... | 2013 | 23994788 |
| a simple and effective set of pcr-based molecular markers for the monitoring of the saccharomyces cerevisiae cell population during bioethanol fermentation. | one of the defining features of the fermentation process used in the production of bioethanol from sugarcane feedstock is the dynamic nature of the yeast population. minisatellite molecular markers are particularly useful for monitoring yeast communities because they produce polymorphic pcr products that typically display wide size variations. we compared the coding sequences derived from the genome of the sugarcane bioethanol strain jay270/pe-2 to those of the reference saccharomyces cerevisiae ... | 2013 | 23994268 |
| predicting greenhouse gas emissions and soil carbon from changing pasture to an energy crop. | bioenergy related land use change would likely alter biogeochemical cycles and global greenhouse gas budgets. energy cane (saccharum officinarum l.) is a sugarcane variety and an emerging biofuel feedstock for cellulosic bio-ethanol production. it has potential for high yields and can be grown on marginal land, which minimizes competition with grain and vegetable production. the daycent biogeochemical model was parameterized to infer potential yields of energy cane and how changing land from gra ... | 2013 | 23991028 |
| screening of trichoderma isolates as a biological control agent against ceratocystis paradoxa causing pineapple disease of sugarcane. | in this study, dual culture, poison agar, and direct methods were used to assess the ability of trichoderma virens imi-392430, t. pseudokoningii imi-392431, t. harzianum imi-392432, t. harzianum imi-392433, and t. harzianum imi-392434 to control ceratocystis paradoxa, which causes the pineapple disease of sugarcane. the highest percentage inhibition of radial growth (pirg) values were observed with t. harzianum imi-392432 using two dual culture methods, 63.80% in method i and 80.82% in method ii ... | 2009 | 23983548 |
| physiology and gene expression profiles of dekkera bruxellensis in response to carbon and nitrogen availability. | the assimilation of nitrate, a nitrogenous compound, was previously described as an important factor favoring dekkera bruxellensis in the competition with saccharomyces cerevisiae for the industrial sugarcane substrate. in this substrate, nitrogen sources are limited and diverse, and a recent report showed that amino acids enable d. bruxellensis to grow anaerobically. thus, understanding the regulation of nitrogen metabolism is one fundamental aspect to comprehend the competiveness of d. bruxell ... | 2013 | 23959165 |
| effect of different substrates and casing materials on the growth and yield of calocybe indica. | calocybe indica, a tropical edible mushroom, is popular because it has good nutritive value and it can be cultivated commercially. the current investigation was undertaken to determine a suitable substrate and the appropriate thickness of casing materials for the cultivation of c. indica. optimum mycelial growth was observed in coconut coir substrate. primordia initiation with the different substrates and casing materials was observed between the 13th and 19th day. the maximum length of stalk wa ... | 2010 | 23956634 |
| characterization and transcriptional analyses of cdnas encoding three trypsin- and chymotrypsin-like proteinases in cry1ab-susceptible and cry1ab-resistant strains of sugarcane borer, diatraea saccharalis. | diatraea saccharalis is a major corn borer pest. midgut serine proteinases are essential for insect growth and development. alteration of midgut proteinases is responsible for bt resistance development in some species. to clone midgut trypsin and chymotrypsin cdnas and to test if the cry1ab resistance in d. saccharalis is associated with changes in midgut proteinases, total midgut tryptic and chymotryptic activities, cdna sequences, and gene expressions of three trypsin and three chymotrypsin ge ... | 2013 | 23955944 |
| selection of reference genes for gene expression studies in virus-infected monocots using quantitative real-time pcr. | both genome-wide transcriptomic surveys of the mrna expression profiles and virus-induced gene silencing-based molecular studies of target gene during virus-plant interaction involve the precise estimation of the transcript abundance. quantitative real-time pcr (qpcr) is the most widely adopted technique for mrna quantification. in order to obtain reliable quantification of transcripts, identification of the best reference genes forms the basis of the preliminary work. nevertheless, the stabilit ... | 2013 | 23954326 |
| direct ethanol production from glucose, xylose and sugarcane bagasse by the corn endophytic fungi fusarium verticillioides and acremonium zeae. | production of ethanol with two corn endophytic fungi, fusarium verticillioides and acremonium zeae, was studied. the yield of ethanol from glucose, xylose and a mixture of both sugars were 0.47, 0.46 and 0.50g/g ethanol/sugar for f. verticillioides and 0.37, 0.39 and 0.48g/g ethanol/sugar for a. zeae. both fungi were able to co-ferment glucose and xylose. ethanol production from 40g/l of pre-treated sugarcane bagasse was 4.6 and 3.9g/l for f. verticillioides and a. zeae, respectively, yielding 0 ... | 2013 | 23942376 |
| an acidic thermostable recombinant aspergillus nidulans endoglucanase is active towards distinct agriculture residues. | aspergillus nidulans is poorly exploited as a source of enzymes for lignocellulosic residues degradation for biotechnological purposes. this work describes the a. nidulans endoglucanase a heterologous expression in pichia pastoris, the purification and biochemical characterization of the recombinant enzyme. active recombinant endoglucanase a (reg a) was efficiently secreted as a 35 kda protein which was purified through a two-step chromatography procedure. the highest enzyme activity was detecte ... | 2013 | 23936633 |
| circadian rhythms of sense and antisense transcription in sugarcane, a highly polyploid crop. | commercial sugarcane (saccharum hybrid) is a highly polyploid and aneuploid grass that stores large amounts of sucrose in its stem. we have measured circadian rhythms of sense and antisense transcription in a commercial cultivar (rb855453) using a custom oligoarray with 14,521 probes that hybridize to sense transcripts (ss) and 7,380 probes that hybridize to antisense transcripts (as).we estimated that 32% of ss probes and 22% as probes were rhythmic. this is a higher proportion of rhythmic prob ... | 2013 | 23936527 |
| involvement of anthocyanins in the resistance to chilling-induced oxidative stress in saccharum officinarum l. leaves. | whether anthocyanins elevate resistance to chilling-induced oxidative stress in saccharum officinarum l. cv badila seedlings is investigated. plants with four fully expanded leaves were exposed to chilling stress (8 °c/4 °c, 11 h photoperiod) for 3 days and then transferred to rewarming condition (25 °c/20 °c, 11 h photoperiod) for another 2 days. at the end of the chilling period, h2o2 and superoxide radical (o2-) levels increased sharply and were near the same in the central (cl) and the final ... | 2013 | 23932150 |
| deciphering the synergism of endogenous glycoside hydrolase families 1 and 9 from coptotermes gestroi. | termites can degrade up to 90% of the lignocellulose they ingest using a repertoire of endogenous and symbiotic degrading enzymes. termites have been shown to secrete two main glycoside hydrolases, which are gh1 (ec 3.2.1.21) and gh9 (ec 3.2.1.4) members. however, the molecular mechanism for lignocellulose degradation by these enzymes remains poorly understood. the present study was conducted to understand the synergistic relationship between gh9 (cgeg1) and gh1 (cgbg1) from coptotermes gestroi, ... | 2013 | 23917163 |
| conversion of c6 and c5 sugars in undetoxified wet exploded bagasse hydrolysates using scheffersomyces (pichia) stipitis cbs6054. | sugarcane bagasse is a potential feedstock for cellulosic ethanol production, rich in both glucan and xylan. this stresses the importance of utilizing both c6 and c5 sugars for conversion into ethanol in order to improve the process economics. during processing of the hydrolysate degradation products such as acetate, 5-hydroxymethylfurfural (hmf) and furfural are formed, which are known to inhibit microbial growth at higher concentrations. in the current study, conversion of both glucose and xyl ... | 2013 | 23895663 |
| proteomic and phytohormone analysis of the response of maize (zea mays l.) seedlings to sugarcane mosaic virus. | sugarcane mosaic virus (scmv) is an important virus pathogen in crop production, causing serious losses in grain and forage yields in susceptible cultivars. control strategies have been developed, but only marginal successes have been achieved. for the efficient control of this virus, a better understanding of its interactions and associated resistance mechanisms at the molecular level is required. | 2013 | 23894637 |