Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| bioaugmentation with butane-utilizing microorganisms to promote in situ cometabolic treatment of 1,1,1-trichloroethane and 1,1-dichloroethene. | a field study was performed to evaluate the potential for in-situ aerobic cometabolism of 1,1,1-trichloroethane (1,1,1-tca) through bioaugmentation with a butane enrichment culture containing predominantly two rhodococcus sp. strains named 179bp and 183bp that could cometabolize 1,1,1-tca and 1,1-dicholoroethene (1,1-dce). batch tests indicated that 1,1-dce was more rapidly transformed than 1,1,1-tca by both strains with 183bp being the most effective organism. this second in a series of bioaugm ... | 2009 | 19022526 |
| a novel nucleoside kinase from burkholderia thailandensis: a member of the phosphofructokinase b-type family of enzymes. | the genome of the mesophilic gram-negative bacterium burkholderia thailandensis contains an open reading frame (i.e. the bth_i1158 gene) that has been annotated as a putative ribokinase and pfk-b family member. notably, although the deduced amino acid sequence of the gene showed only 29% similarity to the recently identified nucleoside kinase from hyperthermophilic archaea methanocaldococcus jannaschii, 15 of 17 residues reportedly involved in the catalytic activity of m. jannaschii nucleoside k ... | 2008 | 19021762 |
| proteomic analysis and immunogenicity of secreted proteins from rhodococcus equi atcc 33701. | rhodococcus equi is one of the most important causes of mortality in foals between 1 and 6 months of age. although rare, infection also occurs in a variety of other mammals including humans, often following immunosuppression of various causes. secreted proteins are known to mediate important pathogen-host interactions and consequently are favored candidates for vaccine development as they are the most easily accessible microbial antigens to the immune system. here, we describe the results of a p ... | 2009 | 19019579 |
| cytokine expression by neutrophils of adult horses stimulated with virulent and avirulent rhodococcus equi in vitro. | rhodococcus equi is an intracellular pathogen of macrophages that causes rhodococcal pneumonia in foals and immunocompromised people. evidence exists that neutrophils play a vital role in resistance to infection with r. equi; however, the means by which neutrophils exert their effects have not been clearly defined. in addition to directly killing bacteria, neutrophils also may exert a protective effect by linking innate and adaptive immune responses. in the present study we evaluated the cytokin ... | 2009 | 19019457 |
| dbt degradation enhancement by decorating rhodococcus erythropolis igst8 with magnetic fe3o4 nanoparticles. | biodesulfurization (bds) of dibenzothiophene (dbt) was carried out by rhodococcus erythropolis igst8 decorated with magnetic fe3o4 nanoparticles, synthesized in-house by a chemical method, with an average size of 45-50 nm, in order to facilitate the post-reaction separation of the bacteria from the reaction mixture. scanning electron microscopy (sem) showed that the magnetic nanoparticles substantially coated the surfaces of the bacteria. it was found that the decorated cells had a 56% higher db ... | 2009 | 19012265 |
| a comparison of various methods to predict bacterial predilection for organic solvents used as reaction media. | bacterial predilection for organic solvents is important in whole-cell biocatalysis in organic media. although various methods of measuring bacterial hydrophobicity have been proposed, it is not fully determined whether they are applicable to the assessment of bacterial predilection for organic solvents in whole-cell biocatalytic processes. in this study, bacterial predilection for organic solvents was assessed by bacterial adhesion to hydrocarbon (bath), contact angle measurement (cam), hydroph ... | 2008 | 19000611 |
| a novel method to generate unmarked gene deletions in the intracellular pathogen rhodococcus equi using 5-fluorocytosine conditional lethality. | a novel method to efficiently generate unmarked in-frame gene deletions in rhodococcus equi was developed, exploiting the cytotoxic effect of 5-fluorocytosine (5-fc) by the action of cytosine deaminase (cd) and uracil phosphoribosyltransferase (uprt) enzymes. the opportunistic, intracellular pathogen r. equi is resistant to high concentrations of 5-fc. introduction of escherichia coli genes encoding cd and uprt conferred conditional lethality to r. equi cells incubated with 5-fc. to exemplify th ... | 2008 | 18984616 |
| assessing soil microbial populations responding to crude-oil amendment at different temperatures using phylogenetic, functional gene (alkb) and physiological analyses. | the effect of temperature as a determinant for selecting microbial populations associated with alkane-degradation was examined in crude oil-amended soil microcosms. after a 30-day incubation, >95% of n-alkane components in the crude-oil were depleted and approximately 40 and 60% of added [14c] hexadecane was converted to 14co2 at 4-10 and 25 degrees c, respectively. concomitant with crude-oil depletion, 16s rrna gene sequence analysis revealed the emergence of a prominent rhodococcus-like 16s rr ... | 2008 | 18983078 |
| if the rumor is tumor, the issue is tissue. | 2008 | 18981853 | |
| activation of peripheral blood monocytes results in more robust production of il-10 in neonatal foals compared to adult horses. | foals are particularly vulnerable to infection by rhodococcus equi during the first 2 weeks of life whereas mature horses are not. while an innate immunodeficiency likely accounts for this clinically relevant vulnerability, the factors that contribute to infection by r. equi have not been fully elucidated. in this study, we demonstrate that cells of the monocyte lineage, including monocytes, macrophages, and dendritic cells, that have been activated with lps and ifn-gamma, respond with a statist ... | 2009 | 18976818 |
| optimization of crucial reaction conditions for the production of nicotinamide by nitrile hydratase using response surface methodology. | the reaction conditions for the nicotinamide production by rhodococcus erythropolis mtcc 1526 have been optimized by statistical experimental design. application of this approach in the bioprocess can result in rallied product yield, reduced development time, and process variability. in this investigation, response surface methodology and central composite design were employed to predict the levels of variables such as reaction ph (6.5, 7, and 7.5), temperature (15, 20, and 25 degrees c), cell c ... | 2008 | 18975144 |
| bioluminescent flow sensor for l-phenylalanine determination in serum. | a highly sensitive and rapid bioluminescent flow sensor was developed for the determination of the content of l-phenylalanine (phe) in serum by monitoring the reduced form of nicotinamide adenine dinucleotide (nadh), produced by immobilized phenylalanine dehydrogenase (phedh), with bacterial bioluminescent enzymes immobilized on a separate nylon coil. the l-phedhs extracted from bacillus badius, bacillus sphaericus and rhodococcus sp. m 4 were investigated and the performances of the three immob ... | 1993 | 18965647 |
| the titanium binding protein of rhodococcus ruber gin1 (ncimb 40340) is a cell-surface homolog of the cytosolic enzyme dihydrolipoamide dehydrogenase. | rhodococcus ruber gin1 (formally rh. strain gin1) was previously isolated on the basis of its strong adherence to coal fly ash (cfa) and titanium dioxide particles from cfa sedimentation ponds of an electrical power plant in israel. the interaction of the bacterium with oxides has been shown to be mediated by a cell surface protein designated tibp (titanium binding protein) involving primarily strong, non-electrostatic forces. in this work, we set forward to identify this unique exocellular prot ... | 2009 | 18956442 |
| the actinobacterial mce4 locus encodes a steroid transporter. | bioinformatic analyses have suggested that mce proteins in diverse actinobacteria are components of complex atp-binding cassette transporter systems, comprising more than eight distinct proteins. in mycobacterium tuberculosis, these proteins are implicated in interactions of this deadly pathogen with its human host. here, we provide direct evidence that the mce4 system of rhodococcus jostii rha1 is a steroid uptake system. transcriptional analyses indicate that the system is encoded by an 11-gen ... | 2008 | 18955493 |
| the gaf-like-domain-containing transcriptional regulator dfdr is a sensor protein for dibenzofuran and several hydrophobic aromatic compounds. | dibenzofuran (df) is one of the dioxin carbon skeletal compounds used as a model to study the microbial degradation of dioxins. this study analyzed the transcriptional regulation of the df dioxygenase genes dfda1 to dfda4 in the df-utilizing actinomycetes rhodococcus sp. strain yk2 and terrabacter sp. strain yk3. an open reading frame designated dfdr was detected downstream of the dfdc genes. the c-terminal part of the dfdr amino acid sequence has high levels of similarity to several luxr-type d ... | 2009 | 18952799 |
| catalytic mechanism of nitrile hydratase proposed by time-resolved x-ray crystallography using a novel substrate, tert-butylisonitrile. | nitrile hydratases (nhases) have an unusual iron or cobalt catalytic center with two oxidized cysteine ligands, cysteine-sulfinic acid and cysteine-sulfenic acid, catalyzing the hydration of nitriles to amides. recently, we found that the nhase of rhodococcus erythropolis n771 exhibited an additional catalytic activity, converting tert-butylisonitrile (tbunc) to tert-butylamine. taking advantage of the slow reactivity of tbunc and the photoreactivity of nitrosylated nhase, we present the first s ... | 2008 | 18948265 |
| specific detection of clavibacter michiganensis subsp. sepedonicus by amplification of three unique dna sequences isolated by subtraction hybridization. | abstract three single-copy, unique dna fragments, designated cms50, cms72, and cms85, were isolated from strain cs3 of clavibacter michiganensis subsp. sepedonicus by subtraction hybridization using driver dna from c. michiganensis subsp. insidiosus, c. michiganensis subsp. michiganensis, and rhodococcus facians. radio-labeled probes made of these fragments and used in southern blot analysis revealed each to be absolutely specific to all north american c. michiganensis subsp. sepedonicus strains ... | 1997 | 18945054 |
| characterization of the microbial community involved in the suppression of pythium aphanidermatum in cucumber grown on rockwool. | abstract the root pathogen pythium aphanidermatum induced lower levels of disease in cucumber (cucumis sativus) plants on unsterilized, re-used rockwool slabs than on heat-sterilized, re-used rockwool. several recolonization treatments of the sterilized rockwool enhanced the suppressiveness of the rockwool. microbial community structures in the different rockwool treatments were investigated by plate counts on selective media. disease suppressiveness in the different rockwool treatments showed t ... | 2005 | 18943014 |
| rhodococcus equi infection in a patient with spinocellular carcinoma of unknown origin. | a rhodococcus equi pulmonary infection in a 63-year-old man receiving chemotherapy and radiotherapy for spinocellular carcinoma is described. the patient, a knife-grinder, was promptly treated with levofloxacin plus amikacin followed by rifampicin for 2 months, and he is still in good clinical condition after an 8-month follow-up. | 2008 | 18927425 |
| trichloroethylene cometabolic degradation by rhodococcus sp. l4 induced with plant essential oils. | cometabolic degradation of tce by toluene-degrading bacteria has the potential for being a cost-effective bioremediation technology. however, the application of toluene may pose environmental problems. in this study, several plant essential oils and their components were examined as alternative inducer for tce cometabolic degradation in a toluene-degrading bacterium, rhodococcus sp. l4. using the initial tce concentration of 80 microm, lemon and lemongrass oil-grown cells were capable of 20 +/- ... | 2009 | 18846429 |
| [diversity of actinomycetes in wuling mountain]. | to isolate new actinomycetes for discovering compounds of pharmaceutical importance. | 2008 | 18837376 |
| isolation of rhodococcus sp. strain ecu0066, a new sulfide monooxygenase-producing strain for asymmetric sulfoxidation. | a new and efficient sulfide monooxygenase-producing strain, ecu0066, was isolated and identified as a rhodococcus sp. that could transform phenylmethyl sulfide (pms) to (s)-sulfoxide with 99% enantiomeric excess via two steps of enantioselective oxidations. its enzyme activity could be effectively induced by adding pms or phenylmethyl sulfoxide (pmso) directly to a rich medium at the early log phase (6 h) of fermentation, resulting in over 10-times-higher production of the enzyme. this bacterial ... | 2009 | 18836022 |
| characterization of a second rhodococcus erythropolis sq1 3-ketosteroid 9alpha-hydroxylase activity comprising a terminal oxygenase homologue, ksha2, active with oxygenase-reductase component kshb. | previously we have characterized 3-ketosteroid 9alpha-hydroxylase (ksh), a key enzyme in microbial steroid degradation in rhodococcus erythropolis strain sq1, as a two-component iron-sulfur monooxygenase, comprised of the terminal oxygenase component ksha1 and the oxygenase-reductase component kshb. deletion of the ksha1 gene resulted in the loss of the ability of mutant strain rg2 to grow on the steroid substrate 4-androstene-3,17-dione (ad). here we report characteristics of a close ksha1 homo ... | 2008 | 18836008 |
| mutual modulation between interleukin-10 and interleukin-6 induced by rhodococcus aurantiacus infection in mice. | the interaction between interleukin-10 (il-10) and interleukin-6 (il-6) was investigated in the inflammatory response to rhodococcus aurantiacus (r. aurantiacus) infection, in which both cytokines act as anti-inflammatory cytokines. compared with wild-type (wt) counterparts, il-6 gene-deficient (il-6(-)/(-)) mice mounted a more robust production of il-10 and tumor necrosis factor-alpha (tnf-alpha) during the initial phase of infection. administration of anti-il-10 antibody resulted in all the mi ... | 2008 | 18834949 |
| characterization of a mycothiol ligase mutant of rhodococcus jostii rha1. | mycothiol (1d-myo-inosityl 2-[n-acetyl-l-cysteinyl]amido-2-deoxy-alpha-d-glucopyranoside) is an important microbial thiol present only in actinomycetes. rhodococcus jostii rha1 degrades a wide range of xenobiotics, including polychlorinated biphenyls, nitriles and n-nitrosodimethylamine. analyses revealed that this strain produces two thiols, mycothiol and ergothioneine, found in the other actinomycetes. a mycothiol ligase mutant strain of r. jostii rha1 deficient in the production of mycothiol ... | 2008 | 18832026 |
| distinct specificities of mycobacterium tuberculosis and mammalian proteasomes for n-acetyl tripeptide substrates. | the proteasome of mycobacterium tuberculosis (mtb) is a validated and drug-treatable target for therapeutics. to lay ground-work for developing peptide-based inhibitors with a useful degree of selectivity for the mtb proteasome over those of the host, we used a library of 5,920 n-acetyl tripeptide-aminomethylcoumarins to contrast the substrate preferences of the recombinant mtb proteasome wild type and open gate mutant, the rhodococcus erythropolis proteasome, and the bovine proteasome with acti ... | 2008 | 18829465 |
| serotypes of rhodococcus equi isolated from horses, immunocompromised human patients and soil in hungary. | two hundred and twelve rhodococcus equi strains were isolated from soil, nasal and rectal swabs of horses and immunocompromised human patients in hungary and serotyped using prescott's serotyping system. one hundred and forty-seven strains (69.3%) belonged to serotype 1, 22 strains (10.4%) to serotype 2, 6 strains (2.8%) to serotype 3 and 1 strain (0.5%) to serotype 4. serotypes 5, 6 and 7 were not found and 36 strains (17%) could not be typed. serotype 1 (72%) was the type most commonly isolate ... | 2008 | 18828479 |
| lack of galectin-3 alters the balance of innate immune cytokines and confers resistance to rhodococcus equi infection. | galectin-3 is a beta-galactoside-binding lectin implicated in the fine-tuning of innate immunity. rhodococcus equi, a facultative intracellular bacterium of macrophages, causes severe granulomatous bronchopneumonia in young horses and immunocompromised humans. the aim of this study is to investigate the role of galectin-3 in the innate resistance mechanism against r. equi infection. the bacterial challenge of galectin-3-deficient mice (gal3-/-) and their wild-type counterpart (gal3+/+) revealed ... | 2008 | 18825751 |
| single nucleotide polymorphisms of interleukin-1 beta related genes and their associations with infection in the horse. | in previous work, we found significant associations of horse chromosome 15 (eca15) microsatellite markers hmso1 and htg06 with two horse infections, rhodococcus equi and lawsonia intracellularis, respectively. interleukin-1 beta subunit and interleukin-1 receptor antagonist encoding genes (il1b and il1rn) could be considered as candidate genes underlying the associations reported. therefore, we identified single nucleotide polymorphisms (snps) within three interleukin-1 beta functionally related ... | 2008 | 18817325 |
| microtitre plate assay for biofilm formation, production and utilization of hydroxybiphenyl by rhodococcus sp. isolated from gasoline-contaminated soil. | gasoline-contaminated soil from isfahan, iran was selected to isolate a bacterium capable of desulfurizing dibenzothiophene (dbt). the isolated strain was named r1 and identified as rhodococcus erythropolis through biochemical tests as well as sequencing of 16s rrna gene. this strain could efficiently produce 2-hydroxybiphenyl (hbp) from dbt via the 4s metabolic pathway. the highest hbp amount was produced at 2 mm dbt with addition of glucose (10 g l(-1)), ethanol (3 g l(-1)), glycerol (2 g l(-1 ... | 2008 | 18811008 |
| tsukamurella: a cause of catheter-related bloodstream infections. | tsukamurellae are strictly aerobic gram-positive rods that can be easily misidentified as corynebacterium species, rhodococcus species, nocardia species, mycobacterium species, or other gram-positive aerobic rods. they have been uncommonly reported as a cause of different human infections, including bloodstream infections. we describe 2 new cases of catheter-related bloodstream infections (cr-bsi) caused by tsukamurella species and review 12 similar cases reported in the literature. conventional ... | 2009 | 18810513 |
| discovery of posttranslational maturation by self-subunit swapping. | several general mechanisms of metallocenter biosynthesis have been reported and reviewed, and in all cases, the components or subunits of an apoprotein remain in the final holoprotein. here, we first discovered that one subunit of an apoenzyme did not remain in the functional holoenzyme. the cobalt-containing low-molecular-mass nitrile hydratase (l-nhase) of rhodococcus rhodochrous j1 consists of beta- and alpha-subunits encoded by the nhlba genes, respectively. an orf, nhle, just downstream of ... | 2008 | 18809911 |
| mechanism of 4-nitrophenol oxidation in rhodococcus sp. strain pn1: characterization of the two-component 4-nitrophenol hydroxylase and regulation of its expression. | 4-nitrophenol (4-np) is a toxic product of the hydrolysis of organophosphorus pesticides such as parathion in soil. rhodococcus sp. strain pn1 degrades 4-np via 4-nitrocatechol (4-nc) for use as the sole carbon, nitrogen, and energy source. a 5-kb ecori dna fragment previously cloned from pn1 contained a gene cluster (nphra1a2) involved in 4-np oxidation. from sequence analysis, this gene cluster is expected to encode an arac/xyls family regulatory protein (nphr) and a two-component 4-np hydroxy ... | 2008 | 18805976 |
| expression of rhodococcus opacus alkb genes in anhydrous organic solvents. | rhodococcus opacus b-4 is a benzene-tolerant bacterium which was isolated from a gasoline-contaminated soil sample. we previously demonstrated that this organism was able to survive and exhibit biocatalytic activity in anhydrous organic solvents for at least 5 d. in the present study, we cloned the alkb1 and alkb2 genes encoding alkane hydroxylases from r. opacus b-4. heterologous expression of the alkb1 and alkb2 genes in escherichia coli jm109 showed that they encode functional alkane hydroxyl ... | 2008 | 18804065 |
| novel catalytic activity of nitrile hydratase from rhodococcus sp. n771. | nitrile hydratase (nhase) from rhodococcus sp. n771 is a non-heme iron enzyme catalyzing the hydration of various nitriles to the corresponding amides. we report a novel catalytic activity of nhase. when nhase was incubated with an large excess of commercially available isovaleronitrile, the charge transfer band from the sulfur ligand to the fe atom shifted from 710 nm to 820 nm, but recovered within 4 min. similar uv-vis absorption changes were observed after the addition of isobutylisonitrile ... | 2008 | 18804061 |
| nitrile-inducible gene expression in mycobacteria. | the ability to ectopically control gene expression is a fundamental tool for the study of bacterial physiology and pathogenesis. while many efficient inducible expression systems are available for gram-negative bacteria, few are useful in phylogenetically distant organisms, such as mycobacteria. we have adapted a highly-inducible regulon of rhodococcus rhodochrous to artificially regulate gene expression in both rapidly-growing environmental mycobacteria and slow-growing pathogens, such as mycob ... | 2009 | 18801704 |
| biodegradation potential of the genus rhodococcus. | a large number of aromatic compounds and organic nitriles, the two groups of compounds covered in this review, are intermediates, products, by-products or waste products of the chemical and pharmaceutical industries, agriculture and the processing of fossil fuels. the majority of these synthetic substances (xenobiotics) are toxic and their release and accumulation in the environment pose a serious threat to living organisms. bioremediation using various bacterial strains of the genus rhodococcus ... | 2009 | 18789530 |
| degradation of various alkyl ethers by alkyl ether-degrading actinobacteria isolated from activated sludge of a mixed wastewater treatment. | various substrate specificity groups of alkyl ether (ae)-degrading actinobacteria coexisted in activated sewage sludge of a mixed wastewater treatment. there were substrate niche overlaps including diethyl ether between linear ae- and cyclic ae-degrading strains and phenetole between monoalkoxybenzene- and linear ae-degrading strains. representatives of each group showed different substrate specificities and degradation pathways for the preferred substrates. determining the rates of initial reac ... | 2008 | 18783815 |
| crystal structure of sco6571 from streptomyces coelicolor a3(2). | sco6571 protein from streptomyces coelicolor a3(2) was overexpressed and purified using rhodococcus erythropolis as an expressing host. crystals of selenomethionine-substituted sco6571 have been obtained by vapor diffusion method. sco6571 crystals diffract to 2.3 a and were found to belong to the orthorhombic space group p2(1)2(1)2(1) with unit cell parameters a = 84.5, b = 171.6, c = 184.8 a. six molecules in the asymmetric unit give a crystal volume per protein mass (v(m)) of 2.97 a (3) da(-1) ... | 2008 | 18782066 |
| hydrophobic bacteria at the hexadecane-water interface: examination of micrometre-scale interfacial properties. | hydrophobic bacteria, like colloidal solids, can spontaneously adsorb onto fluid-fluid interfaces and modify their mechanical properties. in this study, two strains of bacteria--acinetobacter venetianus rag-1 and rhodococcus erythropolis 20s-e1-c--were prepared in their stationary (i.e. non-dividing) phase in the absence of biosurfactants; the cells were then used as emulsifiers to stabilize n-hexadecane droplets in aqueous environments. using the micropipette technique, colloidal stability of t ... | 2008 | 18778923 |
| optimization of nutrient component for diesel oil degradation by rhodococcus erythropolis. | a novel bacterium t7-2 was isolated from the oil-polluted sea-bed mud of bohai sea, northern china, which can degrade diesel oil at 15 degrees c. this bacterium was identified as a strain of rhodococcus erythropolis according to its 16s rdna gene. in order to enhance degradation efficiency, a five-level, three-factor central composite design was employed to optimize the nutrition supplied to artificial seawater. the results indicate that a supplement of 2.53 g (nh(4))(2)so(4)l(-1), 2.75 g na(2)h ... | 2008 | 18778839 |
| update and actual trends on bacterial infections following liver transplantation. | recent advances in effective antimicrobial prophylactic strategies have led to a decline in the incidence of opportunistic infections in liver transplant recipients. however, morbidity and mortality due to infectious diseases remain as major problems. bacterial infections occurring early after transplant are mainly related to the technical aspects of the procedure. by contrast, after the first postoperative days and beyond, the nature and variety of infectious complications change. opportunistic ... | 2008 | 18763277 |
| biodegradation of 2,4,6-trinitrophenol by rhodococcus sp. isolated from a picric acid-contaminated soil. | a picric acid-degrading bacterium, strain njust16, was isolated from a soil contaminated by picric acid and identified as a member of rhodococcus sp. based on 16s rrna sequence. the degradation assays suggested that the strain njust16 could utilize picric acid as the sole source of carbon, nitrogen and energy. the isolate grew optimally at 30 degrees c and initial ph 7.0-7.5 in the mineral salts medium supplemented with picric acid. it was basically consistent with degradation of picric acid by ... | 2009 | 18762376 |
| trisindoline synthesis and anticancer activity. | expression of a rhodococcus-derived oxygenase gene in escherichia coli yielded indigo metabolites with cytotoxic activity against cancer cells. bioactivity-guided fractionation of these indigo metabolites led to the isolation of trisindoline as the agent responsible for the observed in vitro cytotoxic activity against cancer cells. while the cytotoxicity of etoposide, a common anticancer drug, was dramatically decreased in multidrug-resistant (mdr) cancer cells compared with treatment of parenta ... | 2008 | 18760995 |
| fate of the nitrilotriacetic acid-fe(iii) complex during photodegradation and biodegradation by rhodococcus rhodochrous. | aminopolycarboxylic acids are ubiquitous in natural waters and wastewaters. they have the ability to form very stable water-soluble complexes with many metallic di- or trivalent ions. the iron complex nitrilotriacetic acid-fe(iii) (fenta) has been previously shown to increase drastically the rate of photo- and biodegradation of 2-aminobenzothiazole, an organic pollutant, by rhodococcus rhodochrous. for this paper, the fate of fenta was investigated during these degradation processes. first, it w ... | 2008 | 18757580 |
| a novel gene-samfr involved in early stage of streptomyces ansochromogenes differentiation. | a 4.6 kb dna fragment was cloned from the dna library of streptomyces ansochromogenes using a partial dna fragment located in the downstream of promoter-p(th4) as probe. the experiments revealed that this dna fragment consists of saw d gene and a 1.4 kb pvu ii fragment which can accelerate mycelium formation o fs. ansochromogenes. the nucleotide sequence of 1.4 kb dna fragment was determined and analysed; the result indicated that the fragment contains one complete open reading frame (orf) which ... | 1999 | 18726478 |
| role of surfactants in optimizing fluorene assimilation and intermediate formation by rhodococcus rhodochrous vkm b-2469. | biodegradation of fluorene by rhodococcus rhodochrous vkm b-2469 was investigated and optimized by adding non-ionic surfactants to the liquid media. the utilization of 1-1.5% tween 60 or 1% triton x100 allowed to solubilize 1 mm fluorene over 150 times more than in water medium (from 9-11 microm to above 1.5 mm at 28 degrees c). we observed that tween 60 was useful to enhance the fluorene biodegradation rates further supporting r. rhodochrous vkm b-2469 growth as an additional carbon source and ... | 2009 | 18723343 |
| unusual production of glutathione in actinobacteria. | most actinobacteria produce mycothiol as the major thiol. in addition to mycothiol rhodococcus ad45 generates a substantial level of glutathione possibly using genes acquired in a lateral transfer. instead of mycothiol, rubrobacter radiotolerans and rubrobacter xylanophilus produce glutathione, whose synthesis appears to involve enzymes substantially different from those in other organisms. | 2009 | 18719892 |
| biodegradation of p-nitrophenol by rhodococcus sp. cn6 with high cell surface hydrophobicity. | rhodococcus sp. cn6, isolated from a pesticide industry's effluent-sediment, was able to completely degrade and utilize 100mg/l p-nitrophenol (pnp) as the sole carbon, nitrogen and energy sources for growth in the minimal salt media (msm) within 12h. to study the applicability of the strain for bioremediation of pnp, its degradation potential was examined in the presence of different supplemented carbon and nitrogen sources in msm with 100mg/l pnp. dextrin was experienced as the best supplemente ... | 2009 | 18718714 |
| interactions of a rhodococcus sp. biosurfactant trehalose lipid with phosphatidylethanolamine membranes. | trehalose lipids are an important group of glycolipid biosurfasctants mainly produced by rhodococci. beside their known industrial applications, there is an increasing interest in the use of these biosurfactants as therapeutic agents. we have purified a trehalose lipid from rhodococcus sp. and made a detailed study of the effect of the glycolipid on the thermotropic and structural properties of phosphatidylethanolamine membranes of different chain length and saturation, using differential scanni ... | 2008 | 18706388 |
| [images in medicine. cavitary pneumonia due to rhodococcus equi in a patient with aids]. | 2008 | 18689156 | |
| a refinery sludge deposition site: presence of nahh and alkj genes and crude oil biodegradation ability of bacterial isolates. | 204 bacterial isolates from four greek refinery sludge deposition sites were investigated for the presence of nahh and alkj genes encoding key enzymes of both aromatic and aliphatic hydrocarbon degradation pathways by pcr and dna hybridisation. members of pseudomonas, acinetobacter, bacillus, rhodococcus and arthrobacter play important role in bioremediation processes in sandy/loam soil contaminated with oil and nahh and alkj genes were present in the 73% of the isolates. consortia of bacterial ... | 2008 | 18688575 |
| bacterial metabolism of polychlorinated biphenyls. | microbial metabolism is responsible for the removal of persistent organic pollutants including pcbs from the environment. anaerobic dehalogenation of highly chlorinated congeners in aquatic sediments is an important process, and recent evidence has indicated that dehalococcoides and related organisms are predominantly responsible for this process. such anaerobic dehalogenation generates lower chlorinated congeners which are easily degraded aerobically by enzymes of the biphenyl upper pathway (bp ... | 2008 | 18685266 |
| the screening, characterization, and use of omega-laurolactam hydrolase: a new enzymatic synthesis of 12-aminolauric acid. | several omega-laurolactam degrading microorganisms were isolated from soil samples. these strains were capable of growing in a medium containing omega-laurolactam as sole source of carbon and nitrogen. among them, five strains (t7, t31, u124, u224, and u238) were identified as cupriavidus sp. t7, acidovorax sp. t31, cupriavidus sp. u124, rhodococcus sp. u224, and sphingomonas sp. u238, respectively. the omega-laurolactam hydrolyzing enzyme from rhodococcus sp. u224 was purified to homogeneity, a ... | 2008 | 18685217 |
| novel methylotrophic bacteria isolated from the river thames (london, uk). | enrichment and elective culture for methylotrophs from sediment of the river thames in central london yielded a diversity of pure cultures representing several genera of gram-negative and gram-positive bacteria, which were mainly of organisms not generally regarded as typically methylotrophic. substrates leading to successful isolations included methanol, monomethylamine, dimethylamine, trimethylamine, methanesulfonate and dimethylsulfone. several isolates were studied in detail and shown by the ... | 2008 | 18681896 |
| site-specific integration of streptomyces phic31 integrase-based vectors in the chromosome of rhodococcus equi. | streptomyces phic31-based site-specific integration was used to transform the facultative intracellular pathogen rhodococcus equi. the transformation efficiency of vectors incorporating the phic31 integrase and attp sites was comparable to that of replication plasmids using the same electroporation procedure. a single attb integration site was identified within an orf encoding a pirin-like protein, which deviates slightly from the consensus sequence of streptomyces attb sites. vector integration ... | 2008 | 18680524 |
| adp competes with fad binding in putrescine oxidase. | putrescine oxidase from rhodococcus erythropolis ncimb 11540 (puo(rh)) is a soluble homodimeric flavoprotein of 100 kda, which catalyzes the oxidative deamination of putrescine and some other aliphatic amines. the initial characterization of puo(rh) uncovered an intriguing feature: the enzyme appeared to contain only one noncovalently bound fad cofactor per dimer. here we show that this low fad/protein ratio is the result of tight binding of adp, thereby competing with fad binding. ms analysis r ... | 2008 | 18678871 |
| gene cloning, overexpression, and characterization of the nitrilase from rhodococcus rhodochrous tg1-a6 in e. coli. | a dna fragment containing the entire coding sequence of nitrilase gene was amplified from rhodococcus rhodochrous tg1-a6 with high nitrilase activity using pcr and sequenced. the open reading frame of the nitrilase gene contains 1,101 base pairs, which encodes a putative polypeptide of 366 amino acid residues. the nitrilase gene was cloned into an expression vector pet-28a and expressed in an escherichia coli strain bl21(de3). the enzymatic activity of nitrilase, which converts various nitriles ... | 2010 | 18677653 |
| [surfactant production by the rhodococcus erythropolis sh-5 bacterium grown on various carbon sources]. | it has been shown that the rhodococcus erythropolis sh-5 strain can produce surfactants associated and not associated with the cell wall. their content depends on medium composition, the nature of the carbon source, and oxygen supply. the highest biosurfactant (biosf) yield is achieved by growing r. erythropolis sh-5 in medium with 2% kerosene at neutral ph. it has been found that the biosf yield and emulsification index for various hydrocarbons depend on the kind of the nitrogen source used by ... | 2008 | 18669264 |
| cloning and characterization of a gene involved in triacylglycerol biosynthesis and identification of additional homologous genes in the oleaginous bacterium rhodococcus opacus pd630. | the oleaginous bacterium rhodococus opacus strain pd630 serves as a model organism to investigate the metabolism of storage triacylglycerols (tags) in bacteria. the key enzyme catalysing the last step of tag biosynthesis in bacteria is a promiscuous acyltransferase (atf), exhibiting acyl-coa acyltransferase activity to both diacylglycerols (dgat activity) and fatty alcohols (wax ester synthase, ws activity). an 800 bp pcr product was obtained from chromosomal dna of strain pd630 by using degener ... | 2008 | 18667565 |
| [study on the lake baikal microbial community in the areas of the natural oil seeps]. | we studied the composition of a natural microbial community, the distribution of different groups of microorganisms (including those able to degrade oil hydrocarbons) within the areas of natural oil seeps in the lake baikal. it was revealed that, in the bottom sediments, the oil-degrading microorganisms dominating the community have included the bacteria of g. bacillus, while in the water column, dominating microbes are presented by species of genera rhodococcus pseudomonas, and micrococcus. und ... | 2008 | 18663956 |
| [change of oil-degrading activity in microorganisms stored under laboratory conditions]. | change of the oil-degrading activity was studied in psychrophilic microbial strains rhodococcus spp. ds-07, ds-21 and pseudomonas spp. ds-09, ds-22 maintained on various media: rich and synthetic with a selective agent. after 2.5 years of storage on rich medium, the oil-degrading activity decreased by 50-60%, whereas this decrease was insignificant in the medium with oil. passages to selective medium with oil after the storage partly restored the activity. it was found that storage of oil-degrad ... | 2008 | 18663955 |
| biodesulfurization of dibenzothiophene by recombinant gordonia alkanivorans ripi90a. | the dszabc genes from newly reported dibenzothiophene biodesulfurizing bacterium, gordonia alkanivorans ripi90a were cloned and sequenced. the overall nucleotide sequence similarity between the dszabc genes of g. alkanivorans ripi90a and those of rhodococcus erythropolis igts8 and gordonia nitida were 83.1% and 83.2%, respectively. a gene transfer system for g. alkanivorans ripi90a was established employing the escherichia coli-rhodococcus shuttle vector prsg43 as suitable cloning vector, result ... | 2009 | 18653330 |
| detection and identification of bacteria intimately associated with fungi of the order sebacinales. | because of their beneficial impact on plants, the highly diverse mycorrhizal fungi grouped in the order sebacinales lay claim to high ecological and agricultural significance. here, we describe for the first time associations of sebacinoid members with bacteria. using quantitative pcr, denaturating gradient gel electrophoresis and fluorescence in situ hybridization, we detected an intimate association between piriformospora indica and rhizobium radiobacter, an alpha-proteobacterium. the stabilit ... | 2008 | 18637023 |
| biodegradation of paint stripper solvents in a modified gas lift loop bioreactor. | paint stripping wastes generated during the decontamination and decommissioning of former nuclear facilities contain paint stripping organics (dichloromethane, 2-propanol, and methanol) and bulk materials containing paint pigments. it is desirable to degrade the organic residues as part of an integrated chemical-biological treatment system. we have developed a modified gas lift loop bioreactor employing a defined consortium of rhodococcus rhodochrous strain ofs and hyphomicrobium sp. dm-2 that d ... | 1997 | 18636454 |
| enzyme engineering aspects of biocatalysis: cofactor regeneration as example. | reaction engineering is an important tool in the case of cofactor depending enzyme-catalyzed reactions. it allows the establishment of conditions resulting in lower product specific cofactor costs as compared with product-specific enzyme costs. this is shown for the stereospecific reduction of carbonyl compounds yielding chiral amino acids and alcohols. in continuous processes, cofactor costs can be reduced if the cofactor can be retained within the bioreactor or recycled into it after separatio ... | 1996 | 18629898 |
| clinical and microbiological characteristics of community-acquired pneumonia among human immunodeficiency virus-infected patients in northern thailand. | human immunodeficiency virus (hiv) infections are prevalent in thailand. however, the clinical and microbiological characteristics of community-acquired pneumonia (cap) in such patients are not completely clear at present. in the present study, we analyzed the characteristics of cap in 191 hiv-infected patients (192 episodes, 130 males and 61 females, mean age 32.9 years, range: 20-62) who had been admitted to nakornping hospital in northern thailand between december 1996 and january 2002. the m ... | 2008 | 18622672 |
| [isolation, identification and degradation characteristics of a quinoline-degrading bacterium rhodococcus sp ql2]. | a quinoline-degrading bacterium ql2, which utilizes quinoline as sole source of carbon, nitrogen and energy, was isolated from activated sludge in a coke-plant wastewater biological treatment system. according to the morphological characteristics, physiological and biochemical characteristics, and sequence analysis of 16s rrna, the strain was identified as rhodococcus sp.. the optimal temperature, initial ph, and shaker rotary speed for strain ql2 utilizing quinoline are 35-42 degrees c, ph 8-9, ... | 2008 | 18613525 |
| respiratory infections in the immunocompromised patient. | damage to local and systemic defences of the lungs makes the immunocompromised host vulnerable to inhaled microorganisms. the type of underlying disease and its associated immunodeficiency allow a high degree of accurate pathogen prediction. neutropenia is associated with gram-negative bacilli pneumonia. prolonged neutropenia increases the risk of aspergillosis and mucormycosis. cellular immunodeficiency is associated with pneumocystis carinii, legionella spp. and intracellular parasites includi ... | 1993 | 18611585 |
| [alkb homologues in thermophilic bacteria of the genus geobacillus]. | screening of alkane hydroxylase genes (alkb) was performed in the thermophilic aerobic bacteria of the genus geobacillus. total dna was extracted from the biomass of 11 strains grown on the mixture of saturated c10-c20 hydrocarbons, pcr amplification of fragments of alkb genes was performed with degenerate oligonucleotide primers, pcr products were cloned and sequenced. for the first time in the genome of thermophilic bacteria the presence of a set of alkb gene homologues was revealed. the strai ... | 2008 | 18610833 |
| evolution of the rhodococcus equi vap pathogenicity island seen through comparison of host-associated vapa and vapb virulence plasmids. | the pathogenic actinomycete rhodococcus equi harbors different types of virulence plasmids associated with specific nonhuman hosts. we determined the complete dna sequence of a vapb(+) plasmid, typically associated with pig isolates, and compared it with that of the horse-specific vapa(+) plasmid type. pvapb1593, a circular 79,251-bp element, had the same housekeeping backbone as the vapa(+) plasmid but differed over an approximately 22-kb region. this variable region encompassed the vap pathoge ... | 2008 | 18606735 |
| biodesulfurization of water-soluble coal-derived material by rhodococcus rhodochrous igts8. | rhodococcus rhodochrous igts8 was previously isolated because of its ability to use coal as its sole source of sulfur for growth. subsequent growth studies have revealed that igts8 is capable of using a variety of organosulfur compounds as sources of sulfur but not carbon. in this article, the ability of igts8 to selectively remove organic sulfur from water-soluble coal-derived material is investigated. the microbial removal of organic sulfur from coal requires microorganisms capable of cleaving ... | 1992 | 18601220 |
| epoxidation of propene by microbial cells immobilized on inorhanic supports. | immobilization of gas-utilizing microorganism strains (mycobacteria, rhodococcus, methane-utilizers) on inorganic supports based on alumina, silicates, and carbon was carried out to develop heterogeneous biocatalysts for the biotechnologic processes, including the process of propene epoxidation. adsorption ability of these microorganisms, biocatalytic properties of resting and immobilized bacterial cells, and effect of immobilization techniques on biocatalysis were studied. an approach of double ... | 1992 | 18600978 |
| ultrasound enhanced bioprocesses: cholesterol oxidation by rhodococcus erythropolis. | cholesterol oxidation to cholestenone by resting cells of rhodococcus erythropolis atcc25544 was investigated under a computer-controlled ultrasonic irradiation at a frequency of 20 khz. the optimization of the ultrasound intensity and its mode of application to a stirred bioreactor was first established at a level which preserved the structural integrity of the cells and enabled their metabolic activity. a significant enhancement in the kinetic rates of the biotransformation was observed in mic ... | 1988 | 18587766 |
| purification, characterization, and substrate specificity of two 2,3-dihydroxybiphenyl 1,2-dioxygenase from rhodococcus sp. r04, showing their distinct stability at various temperature. | the genes of two 2,3-dihydroxybiphenyl 1,2-dioxygenases (bphc1 and bphc2) were obtained from the gene library of rhodococcus sp. r04. the enzymes have been purified to apparent electrophoretic homogeneity from the cell extracts of the recombinant harboring bphc1 and bphc2. both bphc1 and bphc2 were hexamers, consisting of six subunits of 35 and 33 kda as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, respectively. the enzymes had similar optimal ph (ph 9.0), but differe ... | 2008 | 18585431 |
| cloning and expression of the l-1-amino-2-propanol dehydrogenase gene from rhodococcus erythropolis, and its application to double chiral compound production. | the gene encoding nadp(+)-dependent l-1-amino-2-propanol dehydrogenase (aadh) of rhodococcus erythropolis mak154 was cloned and sequenced. a 780-bp nucleotide fragment was confirmed to be the gene encoding aadh by agreement of the n-terminal and internal amino acid sequences of the purified aadh. the gene (aadh) codes a total of 259 amino acid residues, and the deduced amino acid sequence shows similarity to several short-chain dehydrogenase/reductase family proteins. an expression vector, pkkaa ... | 2008 | 18584170 |
| effects of nicotinamide and riboflavin on the biodesulfurization activity of dibenzothiophene by rhodococcus erythropolis ustb-03. | rhodococcus erythropolis ustb-03 is a promising bacterial strain for the biodesulfurization of dibenzothiophene (dbt) via a sulfur-specific pathway in which dbt is converted to 2-hydroxybiphenyl (2hbp) as an end product. the effects of nicotinamide and riboflavin on the sulfur specific activity (sa) of dbt biodesulfurization by r. erythropolis ustb-03 were investigated. both nicotinamide and riboflavin were found to enhance the expression of sa, which was not previously reported. when r. erythro ... | 2008 | 18575116 |
| rapid detection of microorganisms with nanoparticles and electron microscopy. | rapid detection of microorganisms is highly desirable. a procedure has been developed based on interactions between gold nanoparticles and proteins of microorganisms (escherichia coli, rhodococcus rhodochrous, and candida sp.) followed by scanning electron microscopy (sem). the nanoparticle-cell interaction was confirmed by ultraviolet resonance raman spectroscopy (uvrs) in the sem focus. cell suspensions in a buffer were interacted with gold nanoparticles (<10 nm in diameter) prepared from tetr ... | 2008 | 18570338 |
| biosurfactant production by rhodococcus erythropolis grown on glycerol as sole carbon source. | the production of biosurfactant by rhodococcus erythropolis during the growth on glycerol was investigated. the process was carried out at 28 degrees c in a 1.5-l bioreactor using glycerol as carbon source. the bioprocess was monitored through measurements of biosurfactant concentration and glycerol consumption. after 51 h of cultivation, 1.7 g/l of biosurfactant, surface, and interfacial tensions values (with n-hexadecane) of 43 and 15 mn/m, respectively, 67% of emulsifying index (e (24)), and ... | 2006 | 18563662 |
| improved reactor performance and operability in the biotransformation of carveol to carvone using a solid-liquid two-phase partitioning bioreactor. | in an effort to improve reactor performance and process operability, the microbial biotransformation of (-)-trans-carveol to (r)-(-)-carvone by hydrophobic rhodococcus erythropolis dcl14 was carried out in a two phase partitioning bioreactor (tppb) with solid polymer beads acting as the partitioning phase. previous work had demonstrated that the substrate and product become inhibitory to the organism at elevated aqueous concentrations and the use of an immiscible second phase in the bioreactor w ... | 2008 | 18546419 |
| isolation of alkali-tolerant benzene-degrading bacteria from a contaminated aquifer. | to isolate benzene-degrading strains from neutral and alkaline groundwaters contaminated by benzene, toluene, ethylbenzene, xylenes (btex) from the siren aquifer, uk, and to test their effective ph range and ability to degrade tex. | 2008 | 18544140 |
| high benzene concentrations can favour gram-positive bacteria in groundwaters from a contaminated aquifer. | exposure to pollution exerts strong selective pressure on microbial communities, which may affect their potential to adapt to current or future environmental challenges. in this microcosm study, we used dna fingerprinting based on 16s rrna genes to document the impact of high concentrations of benzene on two bacterial communities from a benzene-contaminated aquifer situated below a petrochemical plant (siren, uk). the two groundwaters harboured distinct aerobic benzene-degrading communities able ... | 2008 | 18540887 |
| rhodococcus kunmingensis sp. nov., an actinobacterium isolated from a rhizosphere soil. | a gram-positive, aerobic, non-motile actinobacterium strain, designated yim 45607(t), was isolated from a rhizosphere soil sample in kunming, south-west china. chemotaxonomically, the isolate contained chemical markers that supported its assignment to the genus rhodococcus. on the basis of 16s rrna gene sequence similarity analysis, strain yim 45607(t) formed a new subline within the genus rhodococcus, with rhodococcus equi as its closest phylogenetic neighbour (98.2 % 16s rrna gene sequence sim ... | 2008 | 18523196 |
| selection and identification of bacterial strains with methyl-tert-butyl ether, ethyl-tert-butyl ether, and tert-amyl methyl ether degrading capacities. | nine bacterial strains isolated from two hydrocarbon-contaminated soils were selected because of their capacity for growth in culture media amended with 200 mg/l of one of the following gasoline oxygenates: methyl-tert-butyl ether (mtbe), ethyl-tert-butyl ether (etbe), and tert-amyl methyl ether (tame). these strains were identified by amplification of their 16s rrna gene, using fdl and rd1 primers, and were tested for their capacity to grow and biotransform these oxygenates in both mineral and ... | 2008 | 18522454 |
| improved biodesulfurization of hydrodesulfurized diesel oil using rhodococcus erythropolis and gordonia sp. | substituted benzothiophenes (bts) and dibenzothiophenes (dbts) remain in diesel oil following conventional desulfurization by hydrodesulfurization. a mixture of washed cells (13.6 g dry cell wt l(-1)) of rhodococcus erythropolis ds-3 and gordonia sp. c-6 were employed to desulfurize hydrodesulfurized diesel oil; its sulfur content was reduced from 1.26 g l(-1) to 180 mg l(-1), approx 86% (w/w) of the total sulfur was removed from diesel oil after three cycles of biodesulfurization. the average d ... | 2008 | 18516503 |
| use of response surface optimization for the production of biosurfactant from rhodococcus spp. mtcc 2574. | the production of biosurfactant from rhodococcus spp. mtcc 2574 was effectively enhanced by response surface methodology (rsm). rhodococcus spp. mtcc 2574 was selected through screening of seven different rhodococcus strains. the preliminary screening experiments (one-factor at a time) suggested that carbon source: mannitol, nitrogen source: yeast extract and meat peptone and inducer: n-hexadecane are the critical medium components. the concentrations of these four media components were optimize ... | 2008 | 18511269 |
| a pulmonary mass caused by rhodococcus equi infection in a renal transplant recipient. | a 62-year-old male kidney transplant recipient was admitted to hospital with a 14-day history of fever, hemoptysis and left-sided pleuritic chest pain. he had suffered malaise, weight loss, night sweats and exertional dyspnea over the previous 3 months. imaging studies of the patient's chest revealed a noncavitated mass measuring 5 x 8 cm in the anterior segment of the left upper lobe of the lung and a left-sided pleural effusion with septa, and bacterial cultures revealed the presence of rhodoc ... | 2008 | 18506169 |
| isolation and characterisation of rhodococcus equi from submaxillary lymph nodes of wild boars (sus scrofa). | rhodococcus equi has been isolated from the submaxillary lymph nodes of domesticated pigs, but little is known about the presence of r. equi in wild boars. the aim of the study was the evaluation of the incidence of r. equi in wild boars and the characterisation of them. of 482 submaxillary lymph nodes of wild boars shot in 39 settlements throughout hungary, r. equi was isolated from 60 specimens, and plasmid types of 82 isolates were examined. the isolates were tested for the presence of 15-17- ... | 2008 | 18499361 |
| chemical composition and antimicrobial activity of the essential oils of the amazon guatteriopsis species. | the essential oils of guatteriopsis blepharophylla, guatteriopsis friesiana and guatteriopsis hispida were obtained by hydrodistillation and analysed by gc and gc/ms. the main compound found in the leaf oil of g. blepharophylla was caryophyllene oxide (1) (69.25%). the leaf oil of g. friesiana contained predominantly beta-eudesmol (2) (51.60%), gamma-eudesmol (3) (23.70%), and alpha-eudesmol (4) (14.56%). the major constituents identified in the leaf of g. hispida were beta-pinene (38.18%), alph ... | 2008 | 18495186 |
| the explosive-degrading cytochrome p450 system is highly conserved among strains of rhodococcus spp. | hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) is a widely used explosive and a serious environmental pollutant. nineteen strains of rhodococcus spp. capable of utilizing rdx as the sole nitrogen source have been isolated. the cytochrome p450 system xpla-xplb, which is responsible for rdx breakdown, is present in 18 of these strains. | 2008 | 18487400 |
| rhodococcus cercidiphylli sp. nov., a new endophytic actinobacterium isolated from a cercidiphyllum japonicum leaf. | an endophytic actinobacterium, designated yim 65003(t), was isolated from a surface sterilized leaf sample of cercidiphyllum japonicum collected from yunnan province, south-west china. the morphological and chemotaxonomic properties of the isolate were typical of members of the genus rhodococcus. analysis of the 16s rrna gene sequence revealed that the isolate was most closely related to rhodococcus fascians dsm 20669(t) (99.6%) and rhodococcus yunnanensis yim 70056(t) (99.0%). dna-dna hybridiza ... | 2008 | 18485653 |
| applicability of the functional gene catechol 1,2-dioxygenase as a biomarker in the detection of btex-degrading rhodococcus species. | catechol 1,2-dioxygenase is a key enzyme in the degradation of monoaromatic pollutants. the detection of this gene is in focus today but recently designed degenerate primers are not always suitable. rhodococcus species are important members of the bacterial community involved in the degradation of aromatic contaminants and their specific detection could help assess functions and activities in the contaminated environments. to reach this aim, specific pcr primer sets were designed for the detecti ... | 2008 | 18479346 |
| enhanced bioproduction of carvone in a two-liquid-phase partitioning bioreactor with a highly hydrophobic biocatalyst. | the microbial biotransformation of (-)-trans-carveol to the flavor and fragrance compound (r)-(-)-carvone by rhodococcus erythropolis dcl14 was carried out in a 3 l two phase partitioning bioreactor with an immiscible liquid second phase in an effort to improve upon the reactor performance achieved in a single aqueous phase system. the purpose of employing the liquid second phase is to minimize biotransformation rate inhibition due to the accumulation of the toxic substrate (cis-carveol) and pro ... | 2008 | 18478563 |
| defective production of interferon-gamma and tumour necrosis factor-alpha by aids mononuclear cells after in vitro exposure to rhodococcus equi. | the production of interferon-gamma and tumour necrosis factor-alpha was evaluated in the peripheral blood mononuclear cells (pbmcs) from healthy donors and aids patients after rhodococcus equi infection in vitro. pbmcs from healthy donors secreted elevated levels of ifn-gamma and tnf-alpha when challenged in vitro with killed r. equi, whereas the release of both cytokines was impaired in supernatant cultures from aids patients. we conclude that the failure of ifn-gamma generation in aids patient ... | 1995 | 18475656 |
| a common soil flagellate (cercomonas sp.) grows slowly when feeding on the bacterium rhodococcus fascians in isolation, but does not discriminate against it in a mixed culture with sphingopyxis witflariensis. | flagellates are very important predators on bacteria in soil. because of their high growth rates, flagellate populations respond rapidly to changes in bacterial numbers. previous results indicate that actinobacteria are generally less suitable than proteobacteria as food for flagellates. in this study, we investigated the growth of the flagellate cercomonas sp. (atcc 50334) on each of the two bacteria sphingopyxis witflariensis (alphaproteobacteria) and rhodococcus fascians (actinobacteria) sepa ... | 2008 | 18462400 |
| nitrile biotransformations for the synthesis of highly enantioenriched beta-hydroxy and beta-amino acid and amide derivatives: a general and simple but powerful and efficient benzyl protection strategy to increase enantioselectivity of the amidase. | biotransformations of a number of racemic beta-hydroxy and beta-amino nitrile derivatives were studied using rhodococcus erythropolis aj270, the nitrile hydratase and amidase-containing microbial whole cell catalyst, under very mild conditions. the overall enantioselectivity of nitrile biotransformations was governed predominantly by the amidase whose enantioselectivity was switched on remarkably by an o- and a n-benzyl protection group of the substrates. while biotransformations of beta-hydroxy ... | 2008 | 18459810 |
| in vitro immunomodulating activity of biosurfactant glycolipid complex from rhodococcus ruber. | the biosurfactant glycolipid complex synthesized by rhodococcus ruber actinobacteria is not toxic and exhibits no appreciable effect on proliferative activity of peripheral blood leukocytes. in the monocyte fraction, the biosurfactant activates the production of il-1beta and tnf-alpha cytokines without modifying the production of il-6. in the mononuclear fraction, the glycolipid biosurfactant exhibited no effects on the production of il-1beta, tnf-alpha, and il-6. these results indicate good pro ... | 2007 | 18457028 |
| [isolation, identification and biodegradation characteristics of a bacterial strain able to degrade nonylphenol]. | a bacteria strain f-10 was isolated from activated carbon, which was processed with sand filtered water from songhua river for a long time, pre-oxidized with ozone, ppc and ferrate. the bacterial strain was capable of utilizing nonylphenol (np) as sole carbon source and identified as rhodococcus erythropolis with the sherlock microbial identification system (mis) from the midi corporation. the optimal conditions for np biodegradation in the shaking flasks were at 30 degrees c and ph 6.0. under t ... | 2008 | 18441946 |
| two angular dioxygenases contribute to the metabolic versatility of dibenzofuran-degrading rhodococcus sp. strain ha01. | rhodococcus sp. strain ha01, isolated through its ability to utilize dibenzofuran (dbf) as the sole carbon and energy source, was also capable, albeit with low activity, of transforming dibenzo-p-dioxin (dd). this strain could also transform 3-chlorodibenzofuran (3cdbf), mainly by angular oxygenation at the ether bond-carrying carbon (the angular position) and an adjacent carbon atom, to 4-chlorosalicylate as the end product. similarly, 2-chlorodibenzofuran (2cdbf) was transformed to 5-chlorosal ... | 2008 | 18441103 |
| chimeric vapa/groel2 dna vaccines enhance clearance of rhodococcus equi in aerosol challenged c3h/he mice. | rhodococcus equi remains a significant bacterial pathogen, causing severe pyogranulomatous pneumonia in foals aged 1-3 months. there is no effective vaccine currently available for the prevention of r. equi pneumonia. dna vaccines are known to offer specific advantages over conventional vaccines. the aim of this study was to demonstrate efficacy of our recombinant dna vaccine candidates, namely pcdna3-re1, pcdna3-re3 and pcdna3-re5 by combining a heat shock protein groel2 to a virulence-associat ... | 2008 | 18423949 |