Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| ip-10 is a sensitive biomarker of antigen recognition in whole-blood stimulation assays used for the diagnosis of mycobacterium bovis infection in african buffaloes (syncerus caffer). | african buffaloes (syncerus caffer) are maintenance hosts of mycobacterium bovis, the causative agent of bovine tuberculosis. they act as reservoirs of this infection for a wide range of wildlife and domestic species, and the detection of infected animals is important to control the geographic spread and transmission of the disease. interferon gamma (ifn-γ) release assays (igras) utilizing pathogen-derived peptide antigens are highly specific tests of m. bovis infection; however, the diagnostic ... | 2015 | 26108287 |
| nkg2d is a key receptor for recognition of bladder cancer cells by il-2-activated nk cells and bcg promotes nk cell activation. | intravesical instillation of bacillus calmette-guérin (bcg) is used to treat superficial bladder cancer, either papillary tumors (after transurethral resection) or high-grade flat carcinomas (carcinoma in situ), reducing recurrence in about 70% of patients. initially, bcg was proposed to work through an inflammatory response, mediated by phagocytic uptake of mycobacterial antigens and cytokine release. more recently, other immune effectors such as monocytes, natural killer (nk), and nkt cells ha ... | 2015 | 26106390 |
| genetics of mycobacterial arabinogalactan and lipoarabinomannan assembly. | the cell wall of mycobacterium tuberculosis is unique in that it differs significantly from those of both gram-negative and gram-positive bacteria. the thick, carbohydrate- and lipid-rich cell wall with distinct lipoglycans enables mycobacteria to survive under hostile conditions such as shortage of nutrients and antimicrobial exposure. the key features of this highly complex cell wall are the mycolyl-arabinogalactan-peptidoglycan (magp)-based and phosphatidyl-myo-inositol-based macromolecular s ... | 2014 | 26104198 |
| age at mycobacterium bovis bcg priming has limited impact on anti-tuberculosis immunity boosted by respiratory mucosal adhu5ag85a immunization in a murine model. | tuberculosis (tb) remains a global pandemic despite the use of bacillus calmette-guérin (bcg) vaccine, partly because bcg fails to effectively control adult pulmonary tb. the introduction of novel boost vaccines such as the human adenovirus 5-vectored adhu5ag85a could improve and prolong the protective immunity of bcg immunization. age at which bcg immunization is implemented varies greatly worldwide, and research is ongoing to discover the optimal stage during childhood to administer the vaccin ... | 2015 | 26098423 |
| spray dried aerosol particles of pyrazinoic acid salts for tuberculosis therapy. [corrected]. | tuberculosis is the most serious infectious disease caused by a single organism, mycobacterium tuberculosis (mtb). the standard of care is a protracted and complex drug treatment regimen made more complicated and of longer duration by the incidence of multiple and extensively drug resistant disease. pulmonary delivery of aerosols as a supplement to the existing regimen offers the advantage of delivering high local drug doses to the initial site of infection and most prominent organ system involv ... | 2015 | 26098136 |
| using a prime and pull approach, lentivector vaccines expressing ag85a induce immunogenicity but fail to induce protection against mycobacterium bovis bacillus calmette-guérin challenge in mice. | although bacillus calmette-guérin (bcg) is an established vaccine with excellent efficacy against disseminated mycobacterium tuberculosis infection in young children, efficacy in adults suffering from respiratory tuberculosis (tb) is suboptimal. prime-boost viral vectored vaccines have been shown to induce effective immune responses and lentivectors (lv) have been shown to improve mucosal immunity in the lung. a mucosal boost to induce local immunogenicity is also referred to as a 'pull' in a pr ... | 2015 | 26095282 |
| infection of a549 human type ii epithelial cells with mycobacterium tuberculosis induces changes in mitochondrial morphology, distribution and mass that are dependent on the early secreted antigen, esat-6. | pulmonary infection by mycobacterium tuberculosis (mtb) involves the invasion of alveolar epithelial cells (aecs). we used mitotracker red(®) to assess changes in mitochondrial morphology/distribution and mass from 6 to 48 h post infection (hpi) by confocal microscopy and flow cytometry in mtb-infected a549 type ii aecs. during early infection there was no effect on mitochondrial morphology, however, by 48 hpi mitochondria appeared fragmented and concentrated around the nucleus. in flow cytometr ... | 2015 | 26092385 |
| bcg vaccines. | bcg is the collective name for a family of live attenuated strains of mycobacterium bovis that are currently used as the only vaccine against tuberculosis (tb). there are two major reasons for studying the genome of these organisms: (i) because they are attenuated, bcg vaccines provide a window into mycobacterium tuberculosis virulence, and (ii) because they have provided protection in several clinical trials and case-control studies, bcg vaccines may shed light on properties required of a tb va ... | 2014 | 26082111 |
| complications following intravesical bacillus calmette-guerin treatment for bladder cancer: a case series of 22 patients. | intravesical bacillus calmette-guerin (bcg) therapy is an effective and widely used treatment for superficial bladder carcinoma. local complications are frequent whereas systemic complications are rare but can be serious, and their management is not well known. | 2015 | 26077036 |
| effects of the fusion design and immunization route on the immunogenicity of ag85a-mtb32 in adenoviral vectored tuberculosis vaccine. | vaccines containing multiple antigens may induce broader immune responses and provide better protection against mycobacterium tuberculosis (mtb) infection as compared to a single antigen. however, strategies for incorporating multiple antigens into a single vector and the immunization routes may affect their immunogenicity. in this study, we utilized recombinant adenovirus type 5 (rad5) as a model vaccine vector, and ag85a (rv3804c) and mtb32 (rv0125) as model antigens, to comparatively evaluate ... | 2015 | 26076321 |
| phosphoproteomic analysis of bacillus calmette-guérin using gel-based and gel-free approaches. | post-translational modifications regulate many aspects of protein behavior and provide options for expanding protein functionality in organisms. protein phosphorylation is one of the major ptms observed in bacteria, which are involved in regulating a myriad of physiological processes. mycobacterium bovis bacillus calmette-guérin (bcg) has been recognized as an important weapon in the fight against tuberculosis (tb) worldwide for over 80 years. in this study, we conducted phosphoproteomic analysi ... | 2015 | 26070398 |
| fecal volatile organic ccompound profiles from white-tailed deer (odocoileus virginianus) as indicators of mycobacterium bovis exposure or mycobacterium bovis bacille calmette-guerin (bcg) vaccination. | white-tailed deer (odocoileus virginianus) serve as a reservoir for bovine tuberculosis, caused by mycobacterium bovis, and can be a source of infection in cattle. vaccination with m. bovis bacille calmette guerin (bcg) is being considered for management of bovine tuberculosis in deer. presently, no method exists to non-invasively monitor the presence of bovine tuberculosis in deer. in this study, volatile organic compound profiles of bcg-vaccinated and non-vaccinated deer, before and after expe ... | 2015 | 26060998 |
| mycolic acids, a promising mycobacterial ligand for targeting of nanoencapsulated drugs in tuberculosis. | the appearance of drug-resistant strains of mycobacterium tuberculosis (mtb) poses a great challenge to the development of novel treatment programmes to combat tuberculosis. since innovative nanotechnologies might alleviate the limitations of current therapies, we have designed a new nanoformulation for use as an anti-tb drug delivery system. it consists of incorporating mycobacterial cell wall mycolic acids (ma) as targeting ligands into a drug-encapsulating poly dl-lactic-co-glycolic acid poly ... | 2015 | 26055640 |
| testing eurasian wild boar piglets for serum antibodies against mycobacterium bovis. | animal tuberculosis (tb) caused by infection with mycobacterium bovis and closely related members of the m. tuberculosis complex (mtc), is often reported in the eurasian wild boar (sus scrofa). tests detecting antibodies against mtc antigens are valuable tools for tb monitoring and control in suids. however, only limited knowledge exists on serology test performance in 2-6 month-old piglets. in this age-class, recent infections might cause lower antibody levels and lower test sensitivity. we exa ... | 2015 | 26051843 |
| computed tomographic findings in cats with mycobacterial infection. | the objective of this study was to describe the ct imaging findings associated with confirmed mycobacterial infection in cats. | 2016 | 26045480 |
| performance of a noninvasive test for detecting mycobacterium bovis shedding in european badger (meles meles) populations. | the incidence of mycobacterium bovis, the causative agent of bovine tuberculosis, in cattle herds in the united kingdom is increasing, resulting in substantial economic losses. the european badger (meles meles) is implicated as a wildlife reservoir and is the subject of control measures aimed at reducing the incidence of infection in cattle populations. understanding the epidemiology of m. bovis in badger populations is essential for directing control interventions and understanding disease spre ... | 2015 | 26041891 |
| intravenous mycobacterium bovis bacillus calmette-guérin ameliorates nonalcoholic fatty liver disease in obese, diabetic ob/ob mice. | inflammation and immune response profoundly influence metabolic syndrome and fatty acid metabolism. to analyze influence of systemic inflammatory response to metabolic syndrome, we inoculated an attenuated vaccine strain of mycobacterium bovis bacillus calmette-guérin (bcg) into leptin-deficient ob/ob mice. bcg administration significantly decreased epididymal white adipose tissue weight, serum insulin levels, and a homeostasis model assessment of insulin resistance. serum high molecular weight ... | 2015 | 26039731 |
| increased vancomycin susceptibility in mycobacteria: a new approach to identify synergistic activity against multidrug-resistant mycobacteria. | mycobacterium tuberculosis is wrapped in complex waxes, impermeable to most antibiotics. comparing mycobacterium bovis bcg and m. tuberculosis mutants that lack phthiocerol dimycocerosates (pdim) and/or phenolic glycolipids with wild-type strains, we observed that glycopeptides strongly inhibited pdim-deprived mycobacteria. vancomycin together with a drug targeting lipid synthesis inhibited multidrug-resistant (mdr) and extensively drug-resistant (xdr) clinical isolates. our study puts glycopept ... | 2015 | 26033733 |
| correction: genomic analysis of a mycobacterium bovis bacillus calmette-guérin strain isolated from an adult patient with pulmonary tuberculosis. | 2015 | 26024365 | |
| ifn-γ differentially regulates subsets of gr-1(+)cd11b(+) myeloid cells in chronic inflammation. | during chronic inflammation, prolonged over-reactive immune response may lead to tissue destruction, while immune suppression hinders tissue repair and pathogen elimination. therefore, precise regulation of the immune response is needed to avoid immuno-pathology. interferon-gamma (ifn-γ) is widely used in clinical treatment of inflammatory diseases. however, the underlying mechanism remains unclear. here, we evaluated the role of ifn-γ on cd11b(+)gr-1(+) myeloid cell differentiation and function ... | 2015 | 26021804 |
| an inducible expression system for high-level expression of recombinant proteins in slow growing mycobacteria. | a novel protein expression vector utilising the inducible hspx promoter of mycobacterium tuberculosis was constructed and evaluated in this study. high-level induction of three mycobacterial antigens, comprising up to 9% of bacterial sonicate, was demonstrated in recombinant mycobacterium bovis bcg when grown under low-oxygen tension, which serves to enhance hspx promoter activity. recombinant proteins were efficiently purified from bacterial lysates in a soluble form by virtue of a c-terminal 6 ... | 2015 | 26021569 |
| mycobacterium bovis enterocolitis in an immunocompromised host. | 2016 | 26020480 | |
| engineering new mycobacterial vaccine design for hiv-tb pediatric vaccine vectored by lysine auxotroph of bcg. | in this study, we have engineered a new mycobacterial vaccine design by using an antibiotic-free plasmid selection system. we assembled a novel escherichia coli (e. coli)-mycobacterial shuttle plasmid p2auxo.hiva, expressing the hiv-1 clade a immunogen hiva. this shuttle vector employs an antibiotic resistance-free mechanism for plasmid selection and maintenance based on glycine complementation in e. coli and lysine complementation in mycobacteria. this plasmid was first transformed into glycine ... | 2014 | 26015961 |
| draft genome sequence of mycobacterium bovis strain sp38, a pathogenic bacterium isolated from a bovine in brazil. | we report a draft genome sequence of mycobacterium bovis strain sp38, isolated from the lungs of a cow in brazil. the assembly of reads resulted in 36 contigs in a total of approximately 4.37 mb. comparison of m. bovis strains sequenced to date will aid in understanding bovine tuberculosis in brazil. | 2015 | 25999553 |
| transgenic cattle produced by nuclear transfer of fetal fibroblasts carrying ipr1 gene at a specific locus. | this study aimed to assess the effects of the intracellular pathogen resistance 1 (ipr1) transgene on preventing infection of mycobacterium bovis in cattle. a specific expression vector for the ipr1 gene was constructed and inserted in the genome between surfactant protein a and methionine adenosyltransferase i of bovine fetal fibroblasts. after scnt, cleavage (86.9% vs. 87.4%, p > 0.05) and blastocyst developmental rates (34.6% vs. 33.5%, p > 0.05) were similar between transgenic and nontransge ... | 2015 | 25998271 |
| mycobacterium bovis infection of cattle and white-tailed deer: translational research of relevance to human tuberculosis. | tuberculosis (tb) is a premier example of a disease complex with pathogens primarily affecting humans (i.e., mycobacterium tuberculosis) or livestock and wildlife (i.e., mycobacterium bovis) and with a long history of inclusive collaborations between physicians and veterinarians. advances in the study of bovine tb have been applied to human tb, and vice versa. for instance, landmark discoveries on the use of koch's tuberculin and interferon-γ release assays for diagnostic purposes, as well as ca ... | 2015 | 25991696 |
| mycobacterium bovis in panama, 2013. | panama remains free of zoonotic tuberculosis caused by mycobacterium bovis. however, dna fingerprinting of 7 m. bovis isolates from a 2013 bovine tuberculosis outbreak indicated minimal homology with strains previously circulating in panama. m. bovis dispersion into panama highlights the need for enhanced genotype testing to track zoonotic infections. | 2015 | 25988479 |
| standing of nucleic acid testing strategies in veterinary diagnosis laboratories to uncover mycobacterium tuberculosis complex members. | nucleic acid testing (nat) designate any molecular approach used for the detection, identification, and characterization of pathogenic microorganisms, enabling the rapid, specific, and sensitive diagnostic of infectious diseases, such as tuberculosis. these assays have been widely used since the 90s of the last century in human clinical laboratories and, subsequently, also in veterinary diagnostics. most nat strategies are based in the polymerase chain reaction (pcr) and its several enhancements ... | 2014 | 25988157 |
| selective breeding: the future of tb management in african buffalo? | the high prevalence of bovine tuberculosis (btb) in african buffalo (syncerus caffer) in regions of southern african has a negative economic impact on the trade of animals and animal products, represents an ecological threat to biodiversity, and poses a health risk to local communities through the wildlife-cattle-human interface. test and cull methods may not be logistically feasible in many free-range wildlife systems, and with the presence of co-existing btb hosts and the limited effectiveness ... | 2015 | 25985909 |
| solution structure of the ndh-1 complex subunit cups from thermosynechococcus elongatus. | the cyanobacterial multi-subunit membrane protein complex ndh-1 is both structurally and functionally related to complex i of eubacteria and mitochondria. in addition to functions in respiration and cyclic electron transfer around photosystem i (psi), the cyanobacterial ndh-1 complex is involved in a unique mechanism for inorganic carbon concentration. although the crystal structures of the similar respiratory complex i from thermus thermophilus and escherichia coli are known, atomic structural ... | 2015 | 25982357 |
| inflammasomes-dependent regulation of il-1β secretion induced by the virulent mycobacterium bovis beijing strain in thp-1 macrophages. | mycobacterium bovis is the causative agent of tuberculosis in cattle. infection of macrophages with m. bovis leads to the activation of the "nucleotide binding and oligomerization, leucine-rich repeat and pyrin domains-containing protein 3" (nlrp3) and "absent in melanoma 2" (aim2) inflammasomes, which in turn triggers release of the proinflammatory cytokine interleukin-1β (il-1β) that contributes to bacterial clearance and plays a crucial role in the host defense. however, nlrp3 and aim2 inflam ... | 2015 | 25980833 |
| differentiation of malignant tumours from granulomas by using dynamic [(18)f]-fluoro-l-α-methyltyrosine positron emission tomography. | previous clinical studies have revealed the potential of [(18)f]-fluoro-l-α-methyltyrosine ((18)f-famt) for the differential diagnosis of malignant tumours from sarcoidosis. however, one concern regarding the differential diagnosis with (18)f-famt is the possibility of false negatives given the small absolute uptake of (18)f-famt that has been observed in some malignant tumours. the aim of this study was to evaluate a usefulness of dynamic (18)f-famt positron emission tomography (pet) for differ ... | 2015 | 25977883 |
| bayesian analysis to evaluate tests for the detection of mycobacterium bovis infection in free-ranging wild bison (bison bison athabascae) in the absence of a gold standard. | we estimated the sensitivity and specificity of the caudal-fold skin test (cft), the fluorescent polarization assay (fpa), and the rapid lateral-flow test (rt) for the detection of mycobacterium bovis in free-ranging wild wood bison (bison bison athabascae), in the absence of a gold standard, by using bayesian analysis, and then used those estimates to forecast the performance of a pairwise combination of tests in parallel. in 1998-99, 212 wood bison from wood buffalo national park (canada) were ... | 2015 | 25973619 |
| prime-boost vaccination with bacillus calmette guerin and a recombinant adenovirus co-expressing cfp10, esat6, ag85a and ag85b of mycobacterium tuberculosis induces robust antigen-specific immune responses in mice. | tuberculosis (tb) remains to be a prevalent health issue worldwide. at present, mycobacterium bovis bacillus calmette guerin (bcg) is the singular anti-tb vaccine available for the prevention of disease in humans; however, this vaccine only provides limited protection against mycobacterium tuberculosis (mtb) infection. therefore, the development of alternative vaccines and strategies for increasing the efficacy of vaccination against tb are urgently required. the present study aimed to evaluate ... | 2015 | 25962477 |
| characterization of genome-wide ordered sequence-tagged mycobacterium mutant libraries by cartesian pooling-coordinate sequencing. | reverse genetics research approaches require the availability of methods to rapidly generate specific mutants. alternatively, where these methods are lacking, the construction of pre-characterized libraries of mutants can be extremely valuable. however, this can be complex, expensive and time consuming. here, we describe a robust, easy to implement parallel sequencing-based method (cartesian pooling-coordinate sequencing or cp-cseq) that reports both on the identity as well as on the location of ... | 2015 | 25960123 |
| comparison of the tuberculin test, histopathological examination, and bacterial culture for the diagnosis of tuberculosis (mycobacterium bovis) in buffaloes (bubalus bubalis) in brazil. | tuberculosis is a disease with a great zoonotic potential. it is considered a major obstacle to cattle production and is responsible for severe losses in several production systems. a comparative cervical test (cct) was performed in 1140 buffaloes from different mesoregions of the state of pará, brazil, with the aim of comparing the sensitivity and specificity of cct with histopathological examination and bacterial culture. of the animals tested using cct, 4.65% (53/1140) were positive, 2.98% (3 ... | 2015 | 25958174 |
| risk factors for visible lesions or positive laboratory tests in bovine tuberculosis reactor cattle in northern ireland. | an observational case-control study was conducted to investigate risk factors for confirmed bovine tuberculosis (btb) infection in cattle reacting positively to the single intradermal comparative cervical test (sicct) in northern ireland in the years 1998, 2002 and 2006. macroscopic lesions were detected at slaughter (positive visible lesion (vl) status) in 43.0% of reactor cattle, whilst 45.3% of those sampled were confirmed as btb positive due to the presence of lesions or positive histopathol ... | 2015 | 25957973 |
| the use of pcr technique in the identification of mycobacterium species responsible for bovine tuberculosis in cattle and buffaloes in pakistan. | bovine tuberculosis is one of the important diseases of dairy and wild animals. the disease is prevalent all over the world, though developed countries have tremendously reduced the prevalence through eradication campaigns. the prevalence of disease in pakistan on the basis of tuberculin testing or culture isolation of the organism has been reported previously. it is, however, important to use the latest diagnostic tools, i.e. pcr to confirm the type of mycobacterium infecting the animals in pak ... | 2015 | 25953024 |
| detection of mycobacterium tuberculosis and mycobacterium avium complexes by real-time pcr in bovine milk from brazilian dairy farms. | foodborne diseases are a public health problem worldwide. the consumption of contaminated raw milk has been recognized as a major cause of transmission of bovine tuberculosis to humans. other mycobacteria that may be present in raw milk and may cause diseases are those belonging to the mycobacterium avium complex. in this study, molecular biology tools were applied to investigate raw milk contamination with mycobacterium spp. in family dairy farms from rio grande do sul, southern brazil. further ... | 2015 | 25951404 |
| mycobacterium bovis bcg vaccination induces divergent proinflammatory or regulatory t cell responses in adults. | mycobacterium bovis bacillus calmette-guérin (bcg), the only currently available vaccine against tuberculosis, induces variable protection in adults. immune correlates of protection are lacking, and analyses on cytokine-producing t cell subsets in protected versus unprotected cohorts have yielded inconsistent results. we studied the primary t cell response, both proinflammatory and regulatory t cell responses, induced by bcg vaccination in adults. twelve healthy adult volunteers who were tubercu ... | 2015 | 25947145 |
| proposal of a screening miru-vntr panel for the preliminary genotyping of mycobacterium bovis in mexico. | mycobacterium bovis is the major causative agent of bovine tuberculosis, one of the most relevant zoonoses in the world, and affects a wide range of wild and domesticated animals. development of screening panels in mycobacterial genotyping, according to specific geographical regions, is strongly needed. the aim of this study is to select a panel, constituted by highly polymorphic miru-vntr loci, to discriminate clinical isolates of m. bovis in mexico. in this study, 65 isolates of m. bovis obtai ... | 2015 | 25945333 |
| essential role of the esx-5 secretion system in outer membrane permeability of pathogenic mycobacteria. | mycobacteria possess different type vii secretion (t7s) systems to secrete proteins across their unusual cell envelope. one of these systems, esx-5, is only present in slow-growing mycobacteria and responsible for the secretion of multiple substrates. however, the role of esx-5 substrates in growth and/or virulence is largely unknown. in this study, we show that esx-5 is essential for growth of both mycobacterium marinum and mycobacterium bovis. remarkably, this essentiality can be rescued by in ... | 2015 | 25938982 |
| transcriptional and proteomic analyses of two-component response regulators in multidrug-resistant mycobacterium tuberculosis. | two-component systems (tcss) have been reported to exhibit a sensing and responding role under drug stress that induces drug resistance in several bacterial species. however, the relationship between tcss and multidrug resistance in mycobacterium tuberculosis has not been comprehensively analysed to date. in this study, 90 m. tuberculosis clinical isolates were analysed using 15-loci mycobacterial interspersed repetitive unit (miru)-variable number tandem repeat (vntr) typing and repetitive extr ... | 2015 | 25937537 |
| epstein-barr virus-induced gene 3 (ebi3) polymorphisms and expression are associated with susceptibility to pulmonary tuberculosis. | tuberculosis (tb) remains a major global health problem and host genetic factors play a critical role in susceptibility and resistance to tb. the aim of this study was to identify novel candidate genes associated with tb susceptibility. we performed a population-based case-control study to genotype 13 tag snps spanning epstein-barr virus-induced gene 3 (ebi3), colony stimulating factor 2 (csf2), il-4, interferon beta 1 (ifnb1), chemokine (c-x-c motif) ligand 14 (cxcl14) and myeloid differentiati ... | 2015 | 25937126 |
| a new lymphocyte proliferation assay for potency determination of bovine tuberculin ppds. | the tuberculin skin test is the method of choice for tuberculosis surveillance in livestock ruminants. the exact definition of the biological activity of bovine tuberculin purified protein derivatives (bovine tuberculin ppds) is essential for the reliability of a test system. ppds consist of heterogeneous mixtures of mycobacterial antigens, making it difficult to determine their potency in vitro. the commonly used batch potency test is therefore based on the evaluation of skin reactions in mycob ... | 2015 | 25935213 |
| anti-dormant mycobacterial activity and target analysis of nybomycin produced by a marine-derived streptomyces sp. | in the course of our search for anti-dormant mycobacterial substances, nybomycin (1) was re-discovered from the culture broth of a marine-derived streptomyces sp. on the bioassay-guided separation. compound 1 showed anti-microbial activity against mycobacterium smegmatis and mycobacterium bovis bcg with the mic of 1.0μg/ml under both actively growing aerobic conditions and dormancy inducing hypoxic conditions. compound 1 is also effective to mycobacterium tuberculosis including the clinically is ... | 2015 | 25934225 |
| granuloma coinfection with mycobacterium bovis, mycobacterium avium subsp. paratuberculosis, and corynebacterium pseudotuberculosis in five hunted red deer (cervus elaphus) in portugal. | we report granulomatous lymphadenitis in red deer (cervus elaphus) in portugal caused by coinfection with corynebacterium pseudotuberculosis, mycobacterium bovis, and mycobacterium avium subsp. paratuberculosis, as demonstrated by molecular methods. | 2015 | 25932668 |
| stable expression of lentiviral antigens by quality-controlled recombinant mycobacterium bovis bcg vectors. | the well-established safety profile of the tuberculosis vaccine strain, mycobacterium bovis bacille calmette-guérin (bcg), makes it an attractive vehicle for heterologous expression of antigens from clinically relevant pathogens. however, successful generation of recombinant bcg strains possessing consistent insert expression has encountered challenges in stability. here, we describe a method for the development of large recombinant bcg accession lots which stably express the lentiviral antigens ... | 2015 | 25924766 |
| comparative ser/thr/tyr phosphoproteomics between two mycobacterial species: the fast growing mycobacterium smegmatis and the slow growing mycobacterium bovis bcg. | ser/thr/tyr protein phosphorylation plays a critical role in regulating mycobacterial growth and development. understanding the mechanistic link between protein phosphorylation signaling network and mycobacterial growth rate requires a global view of the phosphorylation events taking place at a given time under defined conditions. in the present study we employed a phosphopeptide enrichment and high throughput mass spectrometry-based strategy to investigate and qualitatively compare the phosphop ... | 2015 | 25904896 |
| [new insight into bacterial zoonotic pathogens posing health hazards to humans]. | this article presents the problem of evolutionary changes of zoonotic pathogens responsible for human diseases. everyone is exposed to the risk of zoonotic infection, particularly employees having direct contact with animals, i.e. veterinarians, breeders, butchers and workers of animal products' processing industry. the article focuses on pathogens monitored by the european centre for disease prevention and control (ecdc), which has been collecting statistical data on zoonoses from all european ... | 2014 | 25902698 |
| mycobacterium bovis infection in the lion (panthera leo): current knowledge, conundrums and research challenges. | mycobacterium bovis has global public-health and socio-economic significance and can infect a wide range of species including the lion (panthera leo) resulting in tuberculosis. lions are classified as vulnerable under the iucn red list of threatened species and have experienced a 30% population decline in the past two decades. however, no attempt has been made to collate and critically evaluate the available knowledge of m. bovis infections in lions and potential effects on population. in this r ... | 2015 | 25891424 |
| evaluation of miru-vntr for typing of mycobacterium bovis isolated from sika deer in northeast china. | bovine tuberculosis has led to serious economic losses for sika deer producers in china. strategies for controlling the spread of mycobacterium bovis are often hampered by a lack of epidemiological data. specifically, tracing infections requires the ability to trace back infections, which, in turn, requires the ability to determine isolates with a common source. this study was planned to assess the discriminatory power of each mycobacterial interspersed repetitive unit (miru)-variable number tan ... | 2015 | 25889075 |
| investigation of the high rates of extrapulmonary tuberculosis in ethiopia reveals no single driving factor and minimal evidence for zoonotic transmission of mycobacterium bovis infection. | ethiopia, a high tuberculosis (tb) burden country, reports one of the highest incidence rates of extra-pulmonary tb dominated by cervical lymphadenitis (tbln). infection with mycobacterium bovis has previously been excluded as the main reason for the high rate of extrapulmonary tb in ethiopia. | 2015 | 25886866 |
| characterization of effector and memory t cell subsets in the immune response to bovine tuberculosis in cattle. | cultured ifn-γ elispot assays are primarily a measure of central memory t cell (tcm) responses with humans; however, this important subset of lymphocytes is poorly characterized in cattle. vaccine-elicited cultured ifn-γ elispot responses correlate with protection against bovine tuberculosis in cattle. however, whether this assay measures cattle tcm responses or not is uncertain. the objective of the present study was to characterize the relative contribution of tcm (ccr7+, cd62lhi, cd45ro+), t ... | 2015 | 25879774 |
| genomic analysis of a mycobacterium bovis bacillus [corrected] calmette-guérin strain isolated from an adult patient with pulmonary tuberculosis.. | for years, bacillus calmette-guérin (bcg) has served as the unique vaccine against tuberculosis and has generally been regarded as safe. however, a clinical strain labeled 3281 that was isolated from a tb patient was identified to be bcg. via the combination of next-generation sequencing (ngs) and comparative genomic analysis, unique 3281 genetic characteristics were revealed. a region containing the dnaa and dnan genes that is closely related to the initial chromosome replication was found to r ... | 2015 | 25876043 |
| increased protective efficacy of recombinant bcg strains expressing virulence-neutral proteins of the esx-1 secretion system. | mycobacterium bovis bcg is presently the only available anti-tuberculosis vaccine used, worldwide. while bcg protects against miliary tuberculosis (tb) and tuberculoid meningitis in children, it often fails to protect against adult pulmonary tb. it is thus imperative that new improved anti-tb vaccines are developed. the integration of the esx-1 secretion system, absent from bcg due to the deletion of region of difference 1 (rd1), into the genome of bcg has been shown to confer to bcg::esx-1 enha ... | 2015 | 25869896 |
| a case of infectious endocarditis due to bcg. | the occurrence of bacillus calmette-guérin (bcg) disease following instillation for bladder cancer is commonly documented. the intravesical administration of bcg is generally safe, but may present severe complications. a fatal case of native aortic valve infectious endocarditis with septicemia due to bcg in a patient treated with intravesical instillation is reported herein. | 2015 | 25869076 |
| the cyclic di-gmp phosphodiesterase gene rv1357c/bcg1419c affects bcg pellicle production and in vivo maintenance. | bacteria living in a surface-attached community that contains a heterogeneous population, coated with an extracellular matrix, and showing drug tolerance (biofilms) are often linked to chronic infections. in mycobacteria, the pellicle mode of growth has been equated to an in vitro biofilm and meets several of the criteria mentioned above, while tuberculosis infection presents a chronic (latent) phase of infection. as mycobacteria lack most genes required to control biofilm production by other mi ... | 2015 | 25865678 |
| molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis. | to develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. | 2015 | 25865192 |
| evaluation of the efficiency of nested q-pcr in the detection of mycobacterium tuberculosis complex directly from tuberculosis-suspected lesions in post-mortem macroscopic inspections of bovine carcasses slaughtered in the state of mato grosso, brazil. | bovine tuberculosis (btb) is a zoonotic disease caused by mycobacterium bovis, a member of the mycobacterium tuberculosis complex (mtc). the quick and specific detection of this species is of extreme importance, since btb may cause economic impacts, in addition to presenting imminent risks to human health. in the present study a nested real-time pcr test (nested q-pcr) was used in post-mortem evaluations to assess cattle carcasses with btb-suspected lesions. a total of 41,193 cattle slaughtered ... | 2015 | 25863190 |
| some south african rubiaceae tree leaf extracts have antimycobacterial activity against pathogenic and non-pathogenic mycobacterium species. | tuberculosis (tb) caused by mycobacterium tuberculosis remains an ongoing threat to human health. many plant species contain antimycobacterial compounds, which may serve as template molecules for new anti-tb drugs. the rubiaceae family is the largest family of trees in southern africa, and preliminary evidence revealed antimycobacterial activity in several species of the genus, motivating further studies. leaf extracts of 15 tree species from the rubiaceae family were screened for antimycobacter ... | 2015 | 25857273 |
| effects of serial skin testing with purified protein derivative on the level and quality of antibodies to complex and defined antigens in mycobacterium bovis-infected cattle. | several serological tests designed to detect antibodies to immunodominant mycobacterium bovis antigens have recently emerged as ancillary tests for the detection of bovine tuberculosis in cattle, particularly when used after the injection of purified protein derivative (ppd) for skin testing, which significantly boosts m. bovis-specific antibody responses. the present findings demonstrate the onset and duration of boosted antibody responses after the injection of m. bovis ppd for the caudal fold ... | 2015 | 25855555 |
| an evaluation of the use of immunoglobulin a antibody response against mycobacterial antigens for the diagnosis of mycobacterium bovis infection in cattle. | antibody responses are useful indicators of mycobacterium bovis infection in cattle. many studies have evaluated the ability of immunoglobulin g (igg) to serodiagnose bovine tuberculosis (tb). in the current study, immunoglobulin a (iga) and igg responses against the mpb70 and mpb83 antigens of m. bovis, the 38 kda phosphate-binding lipoprotein (psts1) that is a well-known serodiagnostic m. tuberculosis antigen, and a newly identified protein, termed rv1483c, were compared in m. bovis-infected a ... | 2015 | 25855362 |
| [when will the new tuberculosis vaccine appear?]. | the problem of tuberculosis prophylaxis remains actual for many countries of the world including russia. the search of candidates for substitution of the only authorized bcg vaccine has been ongoing for some time, because it does not prevent reactivation of the causative agent in the latent stage and causes generalized bcg-infection in individuals with pronounced immune deficiency. in october 2013 in lille at the european congress "world vaccine 2013" results of multi-year projects and trials of ... | 2015 | 25842959 |
| the buffalo co-infection conundrum. | parasitic worms modulate host immunity, affecting co-infection with mycobacterium bovis (btb). ezenwa and jolles suggested that anthelmintic treatment reduced btb-induced mortality, but increased btb spread. reduced mortality should be weighed against the risk of increasing pathogen spread when adopting this strategy for human treatment of mycobacterium tuberculosis, btb, and hiv. | 2015 | 25818850 |
| guillain-barré syndrome associated with mycobacterium bovis lymphadenitis. | guillain-barré syndrome (gbs) is an autoimmune disease that can be triggered by different infectious agents. here we report the case of a 26-year-old algerian woman who developed gbs associated with a mycobacterium bovis cervical lymphadenitis. following intravenous immunoglobulin therapy, the patient's neurologic state returned to normal after 3 months. the lymphadenitis responded more slowly to the antituberculous treatment and an excision of necrotic cervical lymph nodes had to be performed f ... | 2015 | 25814190 |
| assessment of t cell response to novel mycobacterium tuberculosis synthetic overlapping peptides mixtures (rv2659 and rv2660) and esat-6 in egyptian patients. | tuberculosis (tb) remains one of the major challenges to the global public health. the most powerful tools in any tb control program are prompt diagnosis and successful treatment of patients with active contagious disease. for almost 100 years the tuberculin skin test (tst) has been used to support the diagnosis of active and latent tb infection. the tst has a number of limitations, most notable low specificity in bcg vaccinated individuals due to cross-reactive components in ppd and the mycobac ... | 2014 | 25812354 |
| role for nod2 in mycobacterium tuberculosis-induced inos expression and no production in human macrophages. | m.tb, which causes tb, is a host-adapted intracellular pathogen of macrophages. macrophage intracellular prrs, such as nod proteins, regulate proinflammatory cytokine production in response to various pathogenic organisms. we demonstrated previously that nod2 plays an important role in controlling the inflammatory response and viability of m.tb and mycobacterium bovis bcg in human macrophages. various inflammatory mediators, such as cytokines, ros, and rns, such as no, can mediate this control. ... | 2015 | 25801769 |
| expression of autocrine prolactin and the short isoform of prolactin receptor are associated with inflammatory response and apoptosis in monocytes stimulated with mycobacterium bovis proteins. | increased levels of prolactin (prl) have recently been associated with carcinogenesis and the exacerbation of autoimmune diseases, and might be involved in the progression of tuberculosis (tb). to investigate the relationship between prl and prolactin receptor (prlr) expression with inflammatory response and apoptosis in monocytes, we used thp-1 cells stimulated with antigens of the mycobacterium bovis an5 strain culture filtrate protein (cfp-m. bovis). western blot (wb), real-time polymerase ch ... | 2015 | 25797370 |
| recombinant mycobacterium bovis bcg for immunotherapy in nonmuscle invasive bladder cancer. | in the past three decades, intravesical instillation of mycobacterium bovis bacille calmette-guérin (bcg) has been used for treating bladder cancer and it still remains at the forefront of immunotherapy for cancer patients. although bcg-based therapy is the most effective intravesical therapy for this kind of tumor and represents the only agent known to reduce progression into muscle invasive bladder cancer, bcg is ineffective in approximately 30-40 % of cases and disease recurs in up to 50 % of ... | 2015 | 25794874 |
| childhood bcg vaccination does not influence control of mycobacterium tuberculosis growth by human bronchoalveolar lavage cells. | childhood vaccination with mycobacterium bovis bacille calmette-guérin (bcg) reduces the risk of infection with mycobacterium tuberculosis and the risk of severe forms of tuberculosis in children. the protection of adults from pulmonary tuberculosis is doubtful. this study evaluated the effect of the vaccination on the growth of m. tuberculosis human bronchoalveolar mononuclear cells (balmc). | 2015 | 25794470 |
| methyl-hydroxylamine as an efficacious antibacterial agent that targets the ribonucleotide reductase enzyme. | the emergence of multidrug-resistant bacteria has encouraged vigorous efforts to develop antimicrobial agents with new mechanisms of action. ribonucleotide reductase (rnr) is a key enzyme in dna replication that acts by converting ribonucleotides into the corresponding deoxyribonucleotides, which are the building blocks of dna replication and repair. rnr has been extensively studied as an ideal target for dna inhibition, and several drugs that are already available on the market are used for ant ... | 2015 | 25782003 |
| mycobacterium tuberculosis mce3e suppresses host innate immune responses by targeting erk1/2 signaling. | crucial to the pathogenesis of the tuberculosis (tb)-causing pathogen mycobacterium tuberculosis is its ability to subvert host immune defenses to promote its intracellular survival. the mammalian cell entry protein 3e (mce3e), located in the region of difference 15 of the m. tuberculosis genome and absent in mycobacterium bovis bacillus calmette-guérin, has an essential role in facilitating the internalization of mammalian cells by mycobacteria. however, relatively little is known about the rol ... | 2015 | 25780035 |
| [infection due to mycobacterium bovis in common variable immunodeficiency]. | common variable immunodeficiency (cvid) is an heterogeneous group of disorders characterized by impaired antibody production. it shows a wide spectrum of manifestations including severe and recurrent respiratory infections (streptococcus pneumoniae, haemophilus) and gastrointestinal (campylobacter jejuni, rotavirus and giardia lamblia). viral infections caused by herpes zoster, cytomegalovirus (cmv) and hepatitis c are rare. the opportunistic agents such as cmv, pneumocystis jirovecii, cryptococ ... | 2017 | 25758115 |
| protein energy malnutrition during vaccination has limited influence on vaccine efficacy but abolishes immunity if administered during mycobacterium tuberculosis infection. | protein energy malnutrition (pem) increases susceptibility to infectious diseases, including tuberculosis (tb), but it is not clear how pem influences vaccine-promoted immunity to tb. we demonstrate that pem during low-level steady-state tb infection in a mouse model results in rapid relapse of mycobacterium tuberculosis, as well as increased pathology, in both mycobacterium bovis bcg-vaccinated and unvaccinated animals. pem did not change the overall numbers of cd4 t cells in bcg-vaccinated ani ... | 2015 | 25754202 |
| cpg oligodeoxynucleotides as mucosal adjuvants. | bacterial dna comprising palindromic sequences and containing unmethylated cpg is recognized by toll-like receptor 9 of plasmacytoid dendritic cells (pdcs) and induces the production of interferon-α and chemokines, leading to the activation of a th1 immune response. therefore, synthetic equivalents of bacterial dna (cpg oligodeoxynucleotides) have been developed for clinical applications. they are usually phosphorothioated for in vivo use; this approach also leads to adverse effects as reported ... | 2015 | 25751765 |
| diagnosis of bovine tuberculosis using a metal oxide-based electronic nose. | electronic noses (e-noses) have been used for environmental monitoring, standardization of medicinal flavourings, food safety tests and diagnosis of infectious diseases based on the statistical analysis of volatile organic compounds (vocs). bovine tuberculosis (btb) is officially diagnosed using the intradermal skin test (ist), which is time-consuming and labour-intensive. therefore, a more convenient and rapid test with greater sensitivity would be advantageous as prescreening test. in this stu ... | 2015 | 25739902 |
| evaluation of mycobacterium tuberculosis early secreted antigenic target 6 recombinant protein as a diagnostic marker in skin test. | tuberculosis (tb) is the leading infectious disease in the developing world. delayed-type hypersensitivity skin test diagnoses tb using tuberculin purified protein derivative (ppd), but this test is incapable of distinguishing mycobacterium tuberculosis (mtb) infection from bacillus calmette-guérin (bcg) vaccination or an infection caused by nontuberculous mycobacteria (ntm). this study was performed to evaluate the use of recombinant early secretory antigenic target 6 (resat-6), a secretory pro ... | 2015 | 25737829 |
| pulmonary tuberculosis caused by mycobacterium bovis in china. | the epidemiology of mycobacterium bovis infection in humans in china is unknown. in this study, pulmonary tuberculosis caused by m. bovis in china was studied. a total of 4069 clinical strains isolated from sputa during the 2007-2009 nationwide surveillance of drug-resistant tuberculosis in china were analyzed. m. bovis was identified by para-nitrobenzoic acid and thiophen-2-carboxylic acid hydrazide growth tests, spoligotyping and multiplex pcr amplification. in addition, a total of 1828 clinic ... | 2015 | 25736338 |
| successful treatment of a mycotic multifocal thoracoabdominal aortic aneurysm as a late sequelae of intravesical bacillus calmette-guerin therapy: case report and literature review. | bacillus calmette-guerin (bcg) is a live, attenuated strain of mycobacterium bovis commonly used for the treatment of superficial bladder carcinoma. intravesical bcg is well tolerated despite side effects that range from hematuria to sepsis syndrome. a very rare complication is vascular infection developing a mycotic bcg aneurysm, especially in the thoracoabdominal aorta (taaa). these are challenging to diagnose because of their scarcity, nontraditional culture media, slow growth, and are often ... | 2015 | 25736202 |
| tale nickase-mediated sp110 knockin endows cattle with increased resistance to tuberculosis. | transcription activator-like effector nuclease (talen)-mediated genome modification has been applied successfully to create transgenic animals in various species, such as mouse, pig, and even monkey. however, transgenic cattle with gene knockin have yet to be created using talens. here, we report site-specific knockin of the transcription activator-like effector (tale) nickase-mediated sp110 nuclear body protein gene (sp110) via homologous recombination to produce tuberculosis-resistant cattle. ... | 2015 | 25733846 |
| tuberculosis origin: the neolithic scenario. | this paper follows the dramatic changes in scientific research during the last 20 years regarding the relationship between the mycobacterium tuberculosis complex and its hosts - bovids and/or humans. once the m. tuberculosis and mycobacterium bovis genomes were sequenced, it became obvious that the old story of m. bovis evolving into the human pathogen should be reversed, as m. tuberculosis is more ancestral than m. bovis. nevertheless, the timescale and geographical origin remained an enigma. i ... | 2015 | 25726364 |
| release of mycobacterial antigens. | mycobacterium tuberculosis has evolved from a mycobacterium canettii-like progenitor pool into one of the most successful and widespread human pathogens. the pathogenicity of m. tuberculosis is linked to its ability to secrete/export/release selected mycobacterial proteins, and it is also established that active release of mycobacterial antigens is a prerequisite for strong immune recognition. recent research has enabled mycobacterial secretion systems and vesicle-based release of mycobacterial ... | 2015 | 25703550 |
| a novel dengue virus serotype-2 nanovaccine induces robust humoral and cell-mediated immunity in mice. | dengue virus (denv), a member of the flaviviridae family, can be transmitted to humans through the bite of infected aedes mosquitoes. the incidence of dengue has increased worldwide over the past few decades. inadequate vector control, changing global ecology, increased urbanization, and faster global travel are factors enhancing the rapid spread of the virus and its vector. in the absence of specific antiviral treatments, the search for a safe and effective vaccine grows more imperative. many s ... | 2015 | 25701315 |
| card stabilizes mycobacterial open complexes via a two-tiered kinetic mechanism. | card is an essential and global transcriptional regulator in mycobacteria. while its biological role is unclear, card functions by interacting directly with rna polymerase (rnap) holoenzyme promoter complexes. here, using a fluorescent reporter of open complex, we quantitate rpo formation in real time and show that mycobacterium tuberculosis card has a dramatic effect on the energetics of rnap bound complexes on the m. tuberculosis rrnap3 ribosomal rna promoter. the data reveal that mycobacteriu ... | 2015 | 25697505 |
| spillover of mycobacterium bovis from wildlife to livestock, south africa. | during august 2012-february 2013, bovine tuberculosis was detected in communal livestock bordering the greater kruger national park complex (gknpc) in south africa. using spacer oligonucleotide and variable number tandem repeat typing, we identified the mycobacterium bovis strain endemic in gknpc wildlife. our findings indicate bovine tuberculosis spillover from gknpc wildlife to neighboring livestock. | 2015 | 25695846 |
| potential benefits of cattle vaccination as a supplementary control for bovine tuberculosis. | vaccination for the control of bovine tuberculosis (btb) in cattle is not currently used within any international control program, and is illegal within the eu. candidate vaccines, based upon mycobacterium bovis bacillus calmette-guérin (bcg) all interfere with the action of the tuberculin skin test, which is used to determine if animals, herds and countries are officially btb-free. new diagnostic tests that differentiate infected from vaccinated animals (diva) offer the potential to introduce v ... | 2015 | 25695736 |
| mycobacterium bovis infection in humans and cats in same household, texas, usa, 2012. | mycobacterium bovis infection of cats is exceedingly rare in regions where bovine tuberculosis is not endemic. we describe the diagnosis and clinical management of pulmonary m. bovis infection in 2 indoor-housed cats and their association with at least 1 m. bovis-infected human in texas, usa, in september 2012. | 2015 | 25695666 |
| mycobacterium bovis bcg-associated osteomyelitis/osteitis, taiwan. | 2015 | 25695272 | |
| epidemiology of human mycobacterium bovis disease, california, usa, 2003-2011. | we conducted a retrospective review of california tuberculosis (tb) registry and genotyping data to evaluate trends, analyze epidemiologic differences between adult and child case-patients with mycobacterium bovis disease, and identify risk factors for m. bovis disease. the percentage of tb cases attributable to m. bovis increased from 3.4% (80/2,384) in 2003 to 5.4% (98/1,808) in 2011 (p = 0.002). all (6/6) child case-patients with m. bovis disease during 2010-2011 had >1 parent/guardian who wa ... | 2015 | 25693687 |
| liposome-encapsulated cpg enhances antitumor activity accompanying the changing of lymphocyte populations in tumor via intratumoral administration. | although oligodeoxynucleotides containing cpg motifs (cpg-odn) are potent immune stimulators, the use of natural cpg-odn--phosphodiester-backbone cpg--has been limited due to its instability by nuclease in vivo. the aim of this study is to investigate the anticancer efficiency of cpg-odn capsulated using liposome, which enhances the stability of cpg-odn. we formulated lipoplex, encapsulated natural cpg-odn from mycobacterium bovis with liposome, and tested its immune stimulatory activity in vitr ... | 2015 | 25692533 |
| microrna profiling of the bovine alveolar macrophage response to mycobacterium bovis infection suggests pathogen survival is enhanced by microrna regulation of endocytosis and lysosome trafficking. | mycobacterium bovis, the causative agent of bovine tuberculosis, a major problem for global agriculture, spreads via an airborne route and is taken up by alveolar macrophages (am) in the lung. here, we describe the first next-generation sequencing (rna-seq) approach to temporally profile mirna expression in primary bovine ams post-infection with m. bovis. one, six, and forty mirnas were identified as significantly differentially expressed at 2, 24 and 48 h post-infection, respectively. the diffe ... | 2015 | 25692199 |
| bovine tuberculosis in rwanda: prevalence and economic impact evaluation by meat inspection at société des abattoirs de nyabugogo-nyabugogo abattoir, kigali. | despite the significant public health burden of bovine tuberculosis (btb) in rwanda, the prevalence of btb is poorly documented. this study was conducted to estimate the prevalence of btb in cattle using gross examination of granulomatous lesions, to identify mycobacteria species in suspected samples, and to evaluate the economic impact of meat condemnation based on btb-like lesions in the meat industry in rwanda. routine meat inspection was conducted at société des abattoirs de nyabugogo (saban ... | 2014 | 25685979 |
| mycobacterium bovis infection in a horse with granulomatous enterocolitis. | a 2-year-old dappled percheron horse had a wasting condition that did not respond to antibiotic treatments and ultimately resulted in death. thickening of the wall of the large colon and enlargement of the mesenteric lymph nodes were observed at postmortem examination, along with the presence of pinpoint whitish foci in the liver. microscopic examination of affected tissues revealed diffuse chronic granulomatous enterocolitis, granulomatous mesenteric lymphadenitis, and multifocal granulomatous ... | 2015 | 25677270 |
| extent of mycobacterium bovis transmission among animals of dairy and beef cattle and deer farms in south korea determined by variable-number tandem repeats typing. | identifying sources of mycobacterium bovis transmission would be essential for establishing effective control programs of bovine tuberculosis (tb), a major zoonosis threatening human health worldwide. as an effort to determine the extent of m. bovis transmission among dairy and beef cattle and deer populations, a mycobacterial interspersed repetitive units (miru)-variable-number tandem repeats (vntr) typing method was employed for analysis of 131 m. bovis isolates from 59 holstein dairy cattle, ... | 2015 | 25676210 |
| protective capacity of proteoliposomes from mycobacterium bovis bcg in a mouse model of tuberculosis. | tuberculosis (tb) is one of the most important causes of mortality and morbidity due to infectious diseases. bcg, the vaccine in use, is not fully protective against tb. in a previous study, we have shown that proteoliposomes (outer membrane extracts), obtained from bcg (plbcg) were able to induce humoral immune responses against mycobacterium tuberculosis (mtb) antigens. with the objective to evaluate the protective capability of plbcg alone or as a booster with bcg, a murine model of progressi ... | 2015 | 25671612 |
| quantitative proteomics reveals novel insights into isoniazid susceptibility in mycobacteria mediated by a universal stress protein. | tuberculosis (tb) is caused by the ancient pathogen, mycobacterium tuberculosis, and is one of the most serious infectious diseases in the world. isoniazid (inh) is an important first-line drug for the treatment of active and latent tb. inh resistance is an increasing problem in the treatment of tb. phenotypic resistance to inh, however, is poorly understood. in this study, we constructed a strain of mycobacterium bovis bcg that overexpresses the latency-related universal stress protein (usp), b ... | 2015 | 25664397 |
| genetic evolution of mycobacterium bovis causing tuberculosis in livestock and wildlife in france since 1978. | to study the dynamics of bovine tuberculosis (btb) in france, 4,654 m. bovis strains isolated mainly from livestock and wildlife since 1978 were characterized by spoligotyping and mlva based on miru-vntr. in our study spoligotyping allowed the discrimination of 176 types although 3 spoligotypes are predominant and account for more than half of the total strain population: sb0120 (26%), sb0134 (11%) and sb0121 (6%). in addition, 11% of the isolates, principally from southern france, showing close ... | 2015 | 25658691 |
| mycobacterium bovid infection of total knee arthroplasty after bacille calmette-guérin therapy for bladder cancer. | we present a case of mycobacterium bovis infection of a total knee arthroplasty. this infection developed after use of bacillus calmette-guérin immunotherapy used to treat superficial bladder cancer. the patient presented with joint stiffness. radiographs and inflammatory markers were normal. six weeks after arthroscopy for synovectomy and biopsy, cultures showed m bovis infection of the knee joint. the patient was switched to antitubercular chemotherapy treatment, which resulted in the successf ... | 2015 | 25658082 |
| suppurative supraclavicular bacille calmette-guerine lymphadenitis--a case report, awareness and management options. | diagnosis of bacille calmette-guerine (bcg) adenitis is clinical. conventional laboratory tests do not differentiate bcg adenitis from tuberculous adenitis. we report a case of a 3-month-old healthy baby presenting with suppurative bcg adenitis. fnac revealed afb on zn-staining, later confirmed to be mycobacterium bovis by multiplex pcr. the treatment of suppurative bcg adenitis is needle aspiration. anti-tubercular treatment is unwarranted. | 2015 | 25657133 |