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root associated bacillus sp. improves growth, yield and zinc translocation for basmati rice (oryza sativa) varieties.plant associated rhizobacteria prevailing in different agro-ecosystems exhibit multiple traits which could be utilized in various aspect of sustainable agriculture. two hundred thirty four isolates were obtained from the roots of basmati-385 and basmati super rice varieties growing in clay loam and saline soil at different locations of punjab (pakistan). out of 234 isolates, 27 were able to solubilize zinc (zn) from different zn ores like zinc phosphate [zn3 (po4)2], zinc carbonate (znco3) and z ...201526635754
quaternary ammonium biocides as antimicrobial agents protecting historical wood and brick.quaternary ammonium compounds (qacs) are widely used in disinfection of water, surfaces and instruments as well as in textile, leather and food industries because of their relatively low toxicity, broad antimicrobial spectrum, non-volatility and chemical stability. due to these advantages, qacs are also used in restoration and can be applied on historical material. the aim of this study was to determine the usefulness of biocides based on quaternary ammonium salts and containing various excipien ...201626629794
silver nanoparticles cause complications in pregnant mice.silver nanoparticles (agnps) have attracted much interest and have been used for antibacterial, antifungal, anticancer, and antiangiogenic applications because of their unique properties. the increased usage of agnps leads to a potential hazard to human health. however, the potential effects of agnps on animal models are not clear. this study was designed to investigate the potential impact of agnps on pregnant mice.201526622177
effect of seed pelleting with biocontrol agents on growth and colonisation of roots of mungbean by root-infecting fungi.mungbean (vigna radiata (l.) wilczek) is a leguminous pulse crop that is a major source of proteins, vitamins and minerals. root-infecting fungi produce severe plant diseases like root rot, charcoal rot, damping-off and stem rot. the soil-borne pathogens can be controlled by chemicals, but these chemicals have several negative effects. use of microbial antagonist such as fungi and bacteria is a safe, effective and eco-friendly method for the control of many soil-borne pathogens. biological contr ...201626619828
streptomyces strains producing mitochondriotoxic antimycin a found in cereal grains.reasons for mammalian cell toxicity observed in barley and spring wheat grains were sought. streptomyces sp. isolates from wheat and barley produced heat-stable methanol-soluble substances which inhibited the motility of exposed porcine spermatozoa used as a toxicity indicator. several barley isolates produced antimycin a (2 to 5 ng/mg wet wt of biomass), a macrolide antibiotic known to block oxygen utilization in mitochondria. the antimycin-producing isolates were members of the streptomyces al ...201626619316
bacillus cereus ar156 activates pamp-triggered immunity and induces a systemic acquired resistance through a npr1-and sa-dependent signaling pathway.induced resistance responses play a potent role in plant defense system against pathogen attack. bacillus cereus ar156 is a plant growth promoting rhizobacterium (pgpr) that installs induced systemic resistance (isr) to pseudomonas syringae pv. tomato (pst) in arabidopsis. here, we show that ar156 leaf infiltration enhances disease resistance in arabidopsis through the activation of a systemic acquired resistance (sar). pr1 protein expression and reactive oxygen species (ros) burst are strongly ...201626616055
application of a rapid knowledge synthesis and transfer approach to assess the microbial safety of low-moisture foods.low-moisture foods (lmf) are increasingly implicated in outbreaks of foodborne illness, resulting in a significant public health burden. to inform the development of a new codex alimentarius code of hygienic practice for lmf, we applied a rapid knowledge synthesis and transfer approach to review global research on the burden of illness, prevalence, and interventions to control nine selected microbial hazards in eight categories of lmf. knowledge synthesis methods included an integrated scoping r ...201526613924
changes in the microbial composition of microbrewed beer during the process in the actual manufacturing line.this study investigated changes in the microbial composition of microbrewed beer during the manufacturing processes and identified potential microbial hazards, effective critical quality control points, and potential contamination routes. comprehensive quantitative (aerobic plate count, lactic acid bacteria, fungi, acetic acid bacteria, coliforms, and bacillus cereus) and qualitative (escherichia coli and eight foodborne pathogens) microbiological analyses were performed using samples of raw mat ...201526613919
dual-recognition detection of staphylococcus aureus using vancomycin-functionalized magnetic beads as concentration carriers.vancomycin, which has a strong antibacterial effect to gram-positive bacteria, was adopted as one molecular recognition agent for bacterial detection. magnetic beads (mbs) were functionalized with this antibiotic to effectively concentrate staphylococcus aureus (s. aureus). in addition, alkaline phosphatase (alp)-tagged rabbit immunoglobulin g (alp-igg) was used as the second recognition agent to improve the specificity based on the binding between the fc region of rabbit igg and protein a in th ...201626606309
in vitro evaluation of antimicrobial efficacy of extracts obtained from raw and fermented wild macrofungus, lenzites quercina.in recent time, there is a major concern about antibiotic resistance displayed by some pathogenic microorganisms and this had involved a continuous search for natural antimicrobial products. the phytochemistry as well as antimicrobial activity of extracts obtained from lenzites quercina was investigated. the extracts and purified fractions were, respectively, tested against indicator organisms using agar well diffusion and disc diffusion methods. the quantity of phytochemicals found in the extra ...201526604928
molecular dynamics simulations of bczbp, a deacetylase from bacillus cereus: active site loops determine substrate accessibility and specificity.bczbp is an lmbe-like, homohexameric, zinc-dependent deacetylase from the opportunistic pathogen bacillus cereus with three, thus far uncharacterized, homologues in b. anthracis. although its specific substrate is still unknown, the enzyme has been shown to preferentially deacetylate n-acetylglucosamine and diacetylchitobiose via an active site based on a zinc-binding motif of the type hxddxnh. in the crystal structure, the active site is located at a deep and partially blocked cleft formed at t ...200926602510
characterization and antimicrobial application of biosynthesized gold and silver nanoparticles by using microbacterium resistens.various microorganisms were found to be cable of synthesizing gold and silver nanoparticles when gold and silver salts were supplied in the reaction system. the main objective of this study was to evaluate the extracellular synthesis of gold and silver nanoparticles by the type strain microbacterium resistens(t) [kacc14505]. the biosynthesized gold and silver nanoparticles were characterized by ultraviolet-visible spectroscopy (uv-vis), field emission transmission electron micrograph (fe-tem), e ...201626597594
production of rhamnolipids and diesel oil degradation by bacteria isolated from soil contaminated by petroleum.biosurfactants are microbial secondary metabolites. the most studied are rhamnolipids, which decrease the surface tension and have emulsifying capacity. in this study, the production of biosurfactants, with emphasis on rhamnolipids, and diesel oil degradation by 18 strains of bacteria isolated from waste landfill soil contaminated by petroleum was analyzed. among the studied bacteria, gram-positive endospore forming rods (39%), gram positive rods without endospores (17%), and gram-negative rods ...201626588432
the response of maize (zea mays l.) plant assisted with bacterial consortium and fertilizer under oily sludge.the objective of this study was to evaluate the role of pgpr consortium and fertilizer alone and in combination on the physiology of maize grown under oily sludge stress environment as well on the soil nutrient status. consortium was prepared from bacillus cereus (acc kr232400), bacillus altitudinis (acc kf859970), comamonas (delftia) belonging to family comamonadacea (acc kf859971) and stenotrophomonasmaltophilia (acc kf859973). the experiment was conducted in pots with complete randomized desi ...201626587972
erratum for erill and caruso, complete genome sequence of bacillus cereus group phage tsarbomba. 201526586903
fibrinolytic enzyme production by newly isolated bacillus cereus srm-001 with enhanced in-vitro blood clot lysis potential.the discovery of plasmin-like microbial fibrinolytic enzymes having high specificity and negligible side effects is crucial for thrombolytic therapy. herein, we report one such extra-cellular fibrinolytic enzyme producing bacillus cereus srm-001 isolated from the blood-laden soil of a chicken dump yard. the potency of the enzyme was established with fibrin plate assay and in-vitro blood clot lysis assay. the shake-flask operating parameters and media composition were optimized for maximizing the ...201526582284
phenalenone derivatives and the unusual tricyclic sesterterpene acid from the marine fungus lophiostoma bipolare bcc25910.ten compounds including nine phenalenone derivatives (five bipolarides and four bipolarols) and a sesterterpene acid (bipolarenic acid), were isolated from a marine isolated of the fungus lophiostoma bipolare (bcc25910), along with the known compounds, (-)-scleroderolide, (-)-sclerodin, and oxasetin. chemical structures were elucidated based on nmr spectroscopic data and hresims analysis. in addition, the absolute configurations of the phenalenones were resolved using specific rotations and chem ...201526582262
multiplex detection of nine food-borne pathogens by mpcr and capillary electrophoresis after using a universal pre-enrichment medium.routine microbiological quality analyses in food samples require, in some cases, an initial incubation in pre-enrichment medium. this is necessary in order to ensure that small amounts of pathogenic strains are going to be detected. in this work, a universal pre-enrichment medium has been developed for the simultaneous growth of bacillus cereus, campylobacter jejuni, clostridium perfringens, cronobacter sakazakii, escherichia coli, enterobacteriaceae family (38 species, 27 genera), listeria mono ...201526579100
[screening probiotic endophytic bacteria from medicinal plant flex cornuta and the phytopathogen-inhibiting effect].culturable endophytic bacteria were isolated from medicinal plant ilex cornuta by plate-spreading method, strains with strong inhibitory effect on phytopathogen were screened by confrontation culture and fermentation filtrate culture methods, and the morphological changes of phytopathogen hyphae treated with endophytic bacteria were examined by microscopy and micrograph. their phylogenetic relationships were determined by homology analysis of the 16s rdna sequences of pcr products and the taxono ...201526571677
microgravity-driven remodeling of the proteome reveals insights into molecular mechanisms and signal networks involved in response to the space flight environment.space is a hostile environment characterized by high vacuum, extreme temperatures, meteoroids, space debris, ionospheric plasma, microgravity and space radiation, which all represent risks for human health. a deep understanding of the biological consequences of exposure to the space environment is required to design efficient countermeasures to minimize their negative impact on human health. recently, proteomic approaches have received a significant amount of attention in the effort to further s ...201626571091
the mutation glu151asp in the b-component of the bacillus cereus non-hemolytic enterotoxin (nhe) leads to a diverging reactivity in antibody-based detection systems.the ability of bacillus cereus to cause foodborne toxicoinfections leads to increasing concerns regarding consumer protection. for the diarrhea-associated enterotoxins, the assessment of the non-hemolytic enterotoxin b (nheb) titer determined by a sandwich enzyme immunoassay (eia) correlates best with in vitro cytotoxicity. in general, the regulation of enterotoxin expression of b. cereus is a coordinately-regulated process influenced by environmental, and probably also by host factors. as long ...201526569304
design and synthesis of new 2-aryl-4,5-dihydro-thiazole analogues: in vitro antibacterial activities and preliminary mechanism of action.sixty 2-aryl-4,5-dihydrothiazoles were designed and synthesized in yields ranging from 64% to 89% from cysteine and substituted-benzonitriles via a novel metal- and catalyst-free method. the structures of the title compounds were confirmed mainly by nmr spectral data analysis. antibacterial activity assays showed that the compounds (s)-2-(2'-hydroxyphenyl)-4-hydroxy-methyl- 4,5-dihydrothiazole (7h) and (r)-2-(2'-hydroxyphenyl)-4-hydroxymethyl-4,5-dihydro-thiazole (7h') exhibited significant inhi ...201526569197
binary interactions of antagonistic bacteria with candida albicans under aerobic and anaerobic conditions.we used both aerobic and anaerobic liquid co-cultures, prepared with luria bertani broth, to study the effect of bacteria on the survival of candida albicans in the external environment, away from an animal host. the bacteria were represented by aeromonas hydrophila, bacillus cereus, bacillus subtilis, clostridium, enterobacter, klebsiella pneumoniae, kluyvera ascorbata and serratia marcescens. under aerobic conditions, the yeast's growth was inhibited in the presence of bacterial growth; howeve ...201626566932
complete genome sequence of bacillus cereus forc_005, a food-borne pathogen from the soy sauce braised fish-cake with quail-egg.due to abundant contamination in various foods, the pathogenesis of bacillus cereus has been widely studied in physiological and molecular level. b. cereus forc_005 was isolated from a korean side dish, soy sauce braised fish-cake with quail-egg in south korea. while 21 complete genome sequences of b. cereus has been announced to date, this strain was completely sequenced, analyzed, and compared with other complete genome sequences of b. cereus to elucidate the distinct pathogenic features of a ...201526566422
characterization of wastewater effluents releasing from slaughterhouses in and around hyderabad city (india).the present study was undertaken to determine the pollution load to the environment causing by the wastewater effluents releasing from organized and unorganized slaughterhouses in and around hyderabad city. the wastewater effluents collected from three slaughterhouses, at the sites of releasing out to the surrounding environment,were characterized in terms of physico-chemical and microbiological parameters. the physico-chemical parameters, such as temperature, ph, alkalinity, turbidity, total su ...201426563081
massive horizontal gene transfer, strictly vertical inheritance and ancient duplications differentially shape the evolution of bacillus cereus enterotoxin operons hbl, cytk and nhe.bacillus cereus sensu lato comprises eight closely related species including the human pathogens bacillus anthracis and bacillus cereus. within b. cereus sensu lato, chromosomally and plasmid-encoded toxins exist. while plasmid-mediated horizontal gene transfer of the emetic toxin, anthrax and insecticidal toxins is known, evolution of enterotoxin genes within the group has not been studied.201526555390
antibacterial action against food-borne microorganisms and antioxidant activity of carvacrol-rich oil from lippia origanoides kunth.lippia origanoides kunth from northeast brazil is a plant of pleasant odor used by local people as a food seasoning in substitution the oregano where its carvacrol-rich oil has showed significant antimicrobial activity against human pathogens.201526552874
bacillus cereus adhesion to simulated intestinal mucus is determined by its growth on mucin, rather than intestinal environmental parameters.adhesion of pathogenic bacteria to intestinal mucus, the protective layer of the gastrointestinal epithelium, is often considered a virulence factor. the ability of food-poisoning bacillus cereus strains to attach to mucus and the factors affecting this interaction have not yet been investigated. therefore, the role of adhesion in pathogenesis of b. cereus still remains unknown. in the present study, an in vitro assay based on mucin agar was used to simulate adhesion of b. cereus to mucus. bacte ...201526545170
the bacillus anthracis exosporium: what's the big "hairy" deal?in some bacillus species, including bacillus subtilis, the coat is the outermost layer of the spore. in others, such as the bacillus cereus family, there is an additional layer that envelops the coat, called the exosporium. in the case of bacillus anthracis, a series of fine hair-like projections, also referred to as a "hairy" nap, extends from the exosporium basal layer. the exact role of the exosporium in b. anthracis, or for any of the bacillus species possessing this structure, remains uncle ...201526542035
[screening of molluscacidal microorganisms against oncomelania hupensis and their effect].fifteen soil samples were collected from oncomelania hupensis culture pond in miluo schistosomiasis control and prevention base, hunan province. four strains of bacteria were identified to have molluscacidal effects, numbered as b8, b27, b36 and b59. compared with the fermentation broth groups and bacteria suspension groups, the fermentation supernatant groups of the four strains showed the strongest molluscacidal effect. the fermentation supernatant of b59 strain showed the best molluscacidal e ...201526541047
detection of boron removal capacities of different microorganisms in wastewater and effective removal process.in this study boron removal capacities of different microorganisms were tested. candida tropicalis, rhodotorula mucilaginosa, micrococcus luteus, bacillus thuringiensis, bacillus cereus, bacillus megaterium, bacillus pumilus, pseudomonas aeruginosa and aspergillus versicolor were examined for their boron bioaccumulation capacities in simulated municipal wastewater. a. versicolor and b. cereus were found as the most boron-tolerant microorganisms in the experiments. also boron bioaccumulation yiel ...201526540546
comparison of primary models to predict microbial growth by the plate count and absorbance methods.the selection of a primary model to describe microbial growth in predictive food microbiology often appears to be subjective. the objective of this research was to check the performance of different mathematical models in predicting growth parameters, both by absorbance and plate count methods. for this purpose, growth curves of three different microorganisms (bacillus cereus, listeria monocytogenes, and escherichia coli) grown under the same conditions, but with different initial concentrations ...201526539483
bacteria associated with amblyomma cajennense tick eggs.ticks represent a large group of pathogen vectors that blood feed on a diversity of hosts. in the americas, the ixodidae ticks amblyomma cajennense are responsible for severe impact on livestock and public health. in the present work, we present the isolation and molecular identification of a group of culturable bacteria associated with a. cajennense eggs from females sampled in distinct geographical sites in southeastern brazil. additional comparative analysis of the culturable bacteria from an ...201526537602
evaluation of tools for environmental sampling of bacillus anthracis spores.this study describes the validation of sampling techniques used to detect biological warfare agents used in terror attacks. for this purpose, we tested the efficiencies of different sampling media and extraction solutions for the recovery of bacterial pathogens. we first used bacillus cereus atcc 4342 spores as a surrogate for highly pathogenic b. anthracis to compare recovery efficiencies of spores from four different surfaces. we used three different types of sampling swabs and four different ...201526528669
inhibition of bacillus cereus growth and toxin production by bacillus amyloliquefaciens rd7-7 in fermented soybean products.bacillus cereus is a gram-positive, rod-shaped, spore-forming bacterium that has been isolated from contaminated fermented soybean food products and from the environment. b. cereus produces diarrheal and emetic toxins and has caused many outbreaks of foodborne diseases. in this study, we investigated whether b. amyloliquefaciens rd7-7, isolated from rice doenjang (korean fermented soybean paste), a traditional korean fermented soybean food, shows antimicrobial activity against b. cereus and regu ...201626528531
ces locus embedded proteins control the non-ribosomal synthesis of the cereulide toxin in emetic bacillus cereus on multiple levels.the emetic toxin cereulide produced by bacillus cereus is synthesized by the modular enzyme complex ces that is encoded on a pxo1-like megaplasmid. to decipher the role of the genes adjacent to the structural genes cesa/cesb, coding for the non-ribosomal peptide synthetase (nrps), gene inactivation- and overexpression mutants of the emetic strain f4810/72 were constructed and their impact on cereulide biosynthesis was assessed. the hydrolase cesh turned out to be a part of the complex regulatory ...201526528255
bacillus cereus efflux protein bc3310 - a multidrug transporter of the unknown major facilitator family, umf-2.phylogenetic classification divides the major facilitator superfamily (mfs) into 82 families, including 25 families that are comprised of transporters with no characterized functions. this study describes functional data for bc3310 from bacillus cereus atcc 14579, a member of the "unknown major facilitator family-2" (umf-2). bc3310 was shown to be a multidrug efflux pump conferring resistance to ethidium bromide, sds and silver nitrate when heterologously expressed in escherichia coli dh5α δacrab. ...201526528249
bacillus cereus ar156 primes induced systemic resistance by suppressing mir825/825* and activating defense-related genes in arabidopsis.small rnas play an important role in plant immune responses. however, their regulatory function in induced systemic resistance (isr) is nascent. bacillus cereus ar156 is a plant growth-promoting rhizobacterium that induces isr in arabidopsis against bacterial infection. here, by comparing small rna profiles of pseudomonas syringae pv. tomato (pst) dc3000-infected arabidopsis with and without ar156 pretreatment, we identified a group of arabidopsis micrornas (mirnas) that are differentially regul ...201626526683
identification of novel tylosin analogues generated by a wbla disruption mutant of streptomyces ansochromogenes.streptomyces, as the main source of antibiotics, has been intensively exploited for discovering new drug candidates to combat the evolving pathogens. disruption of wbla, an actinobacteria-specific gene controlling major developmental transition, can cause the alteration of phenotype and morphology in many species of streptomyces. one wbla homologue was found in streptomyces ansochromogenes 7100 by using the basic local alignment search tool. it is interesting to identify whether novel secondary ...201526525981
heavy metal detoxification by different bacillus species isolated from solar salterns.the biosorption mechanism is an alternative for chemical precipitation and ultrafiltration which have been employed to treat heavy metal contamination with a limited success. in the present study, three species of bacillus which were isolated from solar salterns were screened for their detoxification potential of the heavy metals, lead, chromium, and copper, by biosorption. biosorption potential of each isolate was determined by atomic absorption spectroscopy (aas), inductively coupled plasma-op ...201526525498
bacillus cereus infection: 57 case patients and a literature review.we aimed to study the characteristics of patients presenting with a bacillus cereus infection in a university hospital.201626525185
the dna glycosylase alkd uses a non-base-flipping mechanism to excise bulky lesions.threats to genomic integrity arising from dna damage are mitigated by dna glycosylases, which initiate the base excision repair pathway by locating and excising aberrant nucleobases. how these enzymes find small modifications within the genome is a current area of intensive research. a hallmark of these and other dna repair enzymes is their use of base flipping to sequester modified nucleotides from the dna helix and into an active site pocket. consequently, base flipping is generally regarded a ...201526524531
high-level production of bacillus cereus phospholipase c in corynebacterium glutamicum.enzymatic oil degumming (removal of phospholipids) using phospholipase c (plc) is a well-established and environmentally friendly process for vegetable oil refining. in this work, we report the production of recombinant bacillus cereus plc in corynebacterium glutamicum atcc 13869 in a high cell density fermentation process and its performance in soybean oil degumming. a final concentration of 5.5g/l of the recombinant enzyme was achieved when the respective gene was expressed from the tac promot ...201526519562
production of co-polymers of polyhydroxyalkanoates by regulating the hydrolysis of biowastes.production of polyhydroxyalkanoate (pha) co-polymers by bacillus spp. was studied by feeding defined volatile fatty acids (vfas) obtained through controlled hydrolysis of various wastes. eleven mixed hydrolytic cultures (mhcs) each containing 6 strains could generate vfa from slurries of (2% total solids): pea-shells (ps), potato peels (pp), apple pomace (ap) and onion peels (op). ps hydrolysates (obtained with mhc2 and mhc5) inoculated with bacillus cereus egu43 and bacillus thuringiensis egu45 ...201626512866
the exosporium layer of bacterial spores: a connection to the environment and the infected host.much of what we know regarding bacterial spore structure and function has been learned from studies of the genetically well-characterized bacterium bacillus subtilis. molecular aspects of spore structure, assembly, and function are well defined. however, certain bacteria produce spores with an outer spore layer, the exosporium, which is not present on b. subtilis spores. our understanding of the composition and biological functions of the exosporium layer is much more limited than that of other ...201526512126
microbiological quality of raw dried pasta from the german market, with special emphasis on cronobacter species.the microbiological quality of 132 dried pasta products available on the german market, originating from 11 different countries, was studied. sample materials included soft or durum wheat products, some of which produced with other ingredients such as eggs, spices, or vegetables. parameters included hygiene indicators (aerobic plate count, mold count, the presence of enterobacteriaceae) and pathogenic/toxinogenic bacterial species (salmonella spp., staphylococcus aureus, presumptive bacillus cer ...201526509868
role of plant growth promoting rhizobacteria and ag-nano particle in the bioremediation of heavy metals and maize growth under municipal wastewater irrigation.the investigation evaluated the role of plant growth promoting rhizobacteria (pgpr) and ag-nano particle on the growth and metabolism of maize irrigated with municipal wastewater (mw). three pgpr isolated from mw were identified on the basis of 16s-rrna gene sequence analyses as pseudomonas sp., pseudomonas fluorescence, and bacillus cereus. the municipal waste water was used to irrigate the maize seeds inoculated with 3 isolated pgpr. the isolated pgpr had catalase and oxidase enzymes, solubili ...201626507686
the c-terminal zwitterionic sequence of cotb1 is essential for biosilicification of the bacillus cereus spore coat.silica is deposited in and around the spore coat layer of bacillus cereus, and enhances the spore's acid resistance. several peptides and proteins, including diatom silaffin and silacidin peptides, are involved in eukaryotic silica biomineralization (biosilicification). homologous sequence search revealed a silacidin-like sequence in the c-terminal region of cotb1, a spore coat protein of b. cereus. the negatively charged silacidin-like sequence is followed by a positively charged arginine-rich ...201526503850
phytochemical, sub-acute toxicity, and antibacterial evaluation of cordia sebestena leaf extracts.in nigeria, cordia sebestena (boraginaceae), an understudied medicinal plant, is used in traditional medicine for the treatment of gastrointestinal disorders. in this study, we investigated the chemical composition, antibacterial potential, and sub-acute toxicity of c. sebestena leaves.201626501159
proteomics identifies bacillus cereus entd as a pivotal protein for the production of numerous virulence factors.bacillus cereus is a gram-positive pathogen that causes a wide variety of diseases in humans. it secretes into the extracellular milieu proteins that may contribute directly or indirectly to its virulence. entd is a novel exoprotein identified by proteogenomics of b. cereus atcc 14579. we constructed a δentd mutant and analyzed the impact of entd disruption on the cellular proteome and exoproteome isolated from early, late, and stationary-phase cultures. we identified 308 and 79 proteins regulat ...201526500610
antioxidant, antibacterial activity, and phytochemical characterization of melaleuca cajuputi extract.the threat posed by drug-resistant pathogens has resulted in the increasing momentum in research and development for effective alternative medications. the antioxidant and antibacterial properties of phytochemical extracts makes them attractive alternative complementary medicines. therefore, this study evaluated the phytochemical constituents of melaleuca cajuputi flower and leaf (gf and gl, respectively) extracts and their antioxidant and antibacterial activities.201526497742
bacillus cereus nvh 0500/00 can adhere to mucin but cannot produce enterotoxins during gastrointestinal simulation.adhesion to the intestinal epithelium could constitute an essential mechanism of bacillus cereus pathogenesis. however, the enterocytes are protected by mucus, a secretion composed mainly of mucin glycoproteins. these may serve as nutrients and sites of adhesion for intestinal bacteria. in this study, the food poisoning bacterium b. cereus nvh 0500/00 was exposed in vitro to gastrointestinal hurdles prior to evaluation of its attachment to mucin microcosms and its ability to produce nonhemolytic ...201526497468
sporulation temperature reveals a requirement for cote in the assembly of both the coat and exosporium layers of bacillus cereus spores.the bacillus cereus spore surface layers consist of a coat surrounded by an exosporium. we investigated the interplay between the sporulation temperature and the cote morphogenetic protein in the assembly of the surface layers of b. cereus atcc 14579 spores and on the resulting spore properties. the cote deletion affects the coat and exosporium composition of the spores formed both at the suboptimal temperature of 20°c and at the optimal growth temperature of 37°c. transmission electron microsco ...201526497467
inactivation of bacillus cereus spores in a tsuyu sauce using continuous ohmic heating with five sequential elbow-type electrodes.the effect of ohmic heating (oh) in a pilot plant system which had a zig-zag shaped (elbow-type) ohmic heater with five sequential voltage electrodes was investigated on bacillus cereus spores in a commercial tsuyu sauce.201626497155
structural characterization and antimicrobial activity of a biosurfactant obtained from bacillus pumilus dsvp18 grown on potato peels.biosurfactants constitute a structurally diverse group of surface-active compounds derived from microorganisms. they are widely used industrially in various industrial applications such as pharmaceutical and environmental sectors. major limiting factor in biosurfactant production is their production cost.201526495106
endophthalmitis caused by bacillus cereus: a devastating ophthalmological emergency. 201526493084
bioavailability and mobility of organic contaminants in soil: new three-step ecotoxicological evaluation.a novel approach was developed for rapid assessment of bioavailability and potential mobility of contaminants in soil. the response of the same test organism to the organic extract, water extract and solid phase of soil was recorded and compared. this approach was designed to give an initial estimate of the total organic toxicity (response to organic extractable fraction), as well as the mobile (response to water extract) and bioavailable fraction (response to solid phase) of soil samples. eight ...201626490898
inactivation of bacterial pathogens in yoba mutandabota, a dairy product fermented with the probiotic lactobacillus rhamnosus yoba.mutandabota is a dairy product consumed as a major source of proteins and micronutrients in southern africa. in this study the microbial safety of traditional and a variant of mutandabota fermented with the probiotic lactobacillus rhamnosus yoba (yoba mutandabota) was investigated by challenging the products with five important food pathogens: listeria monocytogenes, salmonella spp., campylobacter jejuni, escherichia coli o157:h7 and bacillus cereus. pasteurized full-fat cow's milk was used for ...201626490648
evaluation of bacterial contamination in raw milk, ultra-high temperature milk and infant formula using single molecule, real-time sequencing technology.the pacific biosciences (menlo park, ca) single molecule, real-time sequencing technology (smrt) was reported to have some advantages in analyzing the bacterial profile of environmental samples. in this study, the presence of bacterial contaminants in raw milk, uht milk, and infant formula was determined by smrt sequencing of the full length 16s rrna gene. the bacterial profiles obtained at different taxonomic levels revealed clear differences in bacterial community structure across the 16 analy ...201526476945
synthesis, evaluation and molecular docking studies of amino acid derived n-glycoconjugates as antibacterial agents.six amino acid derived n-glycoconjugates of d-glucose were synthesized, characterized and tested for antibacterial activity against g(+)ve (bacillus cereus) as well as g(-)ve (escherichia coli and klebsiella pneumoniae) bacterial strains. all the tested compounds exhibited moderate to good antibacterial activity against these bacterial strains. the results were compared with the antibacterial activity of standard drug chloramphenicol, where results of a5 (tryptophan derived glycoconjugates) agai ...201526476390
purification and characterization of bacteriocin produced by weissella confusa a3 of dairy origin.a dramatic increase in bacterial resistance towards currently available antibiotics has raised worldwide concerns for public health. therefore, antimicrobial peptides (amps) have emerged as a promisingly new group of therapeutic agents for managing infectious diseases. the present investigation focusses on the isolation and purification of a novel bacteriocin from an indigenous sample of cow milk and it's mode of action. the bacteriocin was isolated from weissella confusa a3 that was isolated fr ...201526474074
enteric bacteria isolated from diarrheal patients in korea in 2014.the aim of this study was to characterize the pathogens responsible for causing diarrhea according to season, region of isolation, patient age, and sex as well as to provide useful data for the prevention of diarrheal disease.201526473090
genome sequences of two bacillus cereus group bacteriophages, eyuki and avesobmore.the genomes of two double-stranded dna (dsdna) bacteriophages isolated on bacillus thuringiensis show similarity to previously sequenced phages and provide evidence of the mosaicism of phage genomes.201526472840
complete genome sequence of bacillus cereus group phage tsarbomba.the bacillus cereus group bacteriophage tsarbomba, a double-stranded dna myoviridae, was isolated from soil collected in saratov, russia. tsarbomba was found to be similar to bacillus phages bcp78 and bcu4, and to have a wide host range among bacillus cereus group species.201526472830
total phenolic and flavonoid content and antibacterial activity of punica granatum l. var. pleniflora flowers (golnar) against bacterial strains causing foodborne diseases.flowers of punica granatum l. (punicaceae) var. pleniflora, known as "golnar" in iranian traditional medicine have been used for the prevention and treatment of foodborne diseases. in this study, antibacterial activities of ethanol extract of golnar and its fractions were scientifically evaluated against bacteria causing foodborne diseases including staphylococcus aureus, bacillus cereus, listeria monocytogenes, escherichia coli, shigella dysantriae, and salmonella typhi. the total phenolic and ...201526470879
antioedematogenic activity, acetylcholinesterase inhibition and antimicrobial properties of jacaranda oxyphylla.jacaranda oxyphylla cham. (bignoniaceae) is a shrub found in the brazilian cerrado and used in folk medicine to treat microbial infections. the aim of this study was to carry out a phytochemical screening and evaluate antioedematogenic, antimicrobial and antiacetylcholinesterase properties of j. oxyphylla crude extracts. all extracts analysed showed presence of terpenoids, which are potentially active chemical substances. a high ache inhibitory activity for hexane extract from leaves and for the ...201626469996
biomineralization of pb(ii) into pb-hydroxyapatite induced by bacillus cereus 12-2 isolated from lead-zinc mine tailings.the remediation of pb(ii) through biomineralization is rergarded as a promising technique as well as an interesting phenomenon for transforming heavy metals from mobile species into very stable minerals in the environment. studies are well needed for in-depth understanding the mechanism of pb(ii) immobilized by bacteria. in the present study, we investigated the uptake and biomineralization of pb(ii) using bacillus cereus 12-2 isolated from lead-zinc mine tailings. the maximum pb(ii) uptake capa ...201626468754
characterization of microfouling and corrosive bacterial community of a firewater distribution system.this investigation provides generic information on the culturable corrosive and the microfouling bacterial community in a firewater distribution system that uses freshwater. conventional microbiological methods were used for the selective isolation of the major microfouling bacteria. the isolates were characterized by 16s rrna gene sequencing and the biofilm as well as the corrosion characteristics of the isolates were evaluated. pseudomonas aeruginosa and bacillus cereus were predominantly obse ...201626467696
effects of temperature and ph on the activities of catechol 2,3-dioxygenase obtained from crude oil contaminated soil in ilaje, ondo state, nigeria.enrichment technique was employed for the isolation of the crude oil degrading bacteria. the isolated bacteria were screened for their degradative ability and the best degrading bacteria were selected based on their growth. specific activities of catechol-2,3-dioxygenase and effects of temperature and ph and their stabilities on the enzyme relative activities were observed. bacteria isolated from the soil sample include; bacillus cereus, b. amyloliquficiens, b. firmus, acinetobacter calcoaceticu ...201526464607
differential oxidative stress responses in castor semilooper, achaea janata.balance between reactive oxygen species (ros) and the antioxidant (ao) defense mechanisms is vital for organism survival. insects serve as an ideal model to elucidate oxidative stress responses as they are prone to different kinds of stress during their life cycle. the present study demonstrates the modulation of ao enzyme gene expression in the insect pest, achaea janata (castor semilooper), when subjected to different oxidative stress stimuli. antioxidant enzymes' (catalase (cat), superoxide d ...201526455997
diversity, antimicrobial and antioxidant activities of culturable bacterial endophyte communities in aloe vera.twenty-nine culturable bacterial endophytes were isolated from surface-sterilized tissues (root, stem and leaf) of aloe vera and molecularly characterized to 13 genera: pseudomonas, bacillus, enterobacter, pantoea, chryseobacterium, sphingobacterium, aeromonas, providencia, cedecea, klebsiella, cronobacter, macrococcus and shigella. the dominant genera include bacillus (20.7%), pseudomonas (20.7%) and enterobacter (13.8%). the crude and ethyl acetate fractions of the metabolites of six isolates, ...201526454221
novel diesel-oil-degrading bacteria and fungi from the ecuadorian amazon rainforest.isolating new diesel-oil-degrading microorganisms from crude-oil contaminated sites and evaluating their degradation capacities are vitally important in the remediation of oil-polluted environments and crude-oil exploitation. in this research, new hydrocarbon-degrading bacteria and fungi were isolated from the crude-oil contaminated soil of the oil-fields in the amazon rainforest of north-east ecuador by using a soil enrichment technique. degradation analysis was tracked by gas chromatography an ...201526442498
rapid enhanced photocatalytic degradation of dyes using novel n-doped zro2.a novel n-doped zro2 (n-zro2) photocatalyst is synthesized through thermal decomposition of zirconium hydroxide-urea complex and is characterized using various techniques, including xrd, ftir, tga, sem, tem, uv-drs, xps, xanes, and bet. the n-zro2 possesses pure monoclinic structure with high crystallinity. by using the proposed facile route of synthesis, both interstitial and substitutional n doping with high dopant stability can be realized. the optical properties of the catalyst are significa ...201626439860
recent research progress with phospholipase c from bacillus cereus.phospholipase c (plc) catalyzes the hydrolysis of phospholipids to produce phosphate monoesters and diacylglycerol. it has many applications in the enzymatic degumming of plant oils. plc bc , a bacterial plc from bacillus cereus, is an optimal choice for this activity in terms of its wide substrate spectrum, high activity, and approved safety. unfortunately, its large-scale production and reliable high-throughput screening of plc bc remain challenging. herein, we summarize the research progress ...201626437973
[noma/cancrum oris: a neglected disease].noma is an aggressive orofacial gangrenous pathology that damages hard and soft tissues of the mouth and the face. throughout the centuries it has been present around the globe, but nowadays it has practically disappeared from developed countries and mainly affects children from the most disadvantaged places, especially in africa. noma disease is a multifactorial process; malnutrition, debilitating diseases (bacterial or viral systemic diseases, hiv-associated immunosuppression, etc.) and intrao ...201526437752
short communication: elucidation of bacterial community structure on thin-spined porcupine (chaetomys subspinosus) spines by denaturing.thin-spined porcupines (chaetomys subspinosus) are threatened with extinction and are categorized as vulnerable. this is because of alteration to and loss of their habitat and possible hunting activities in their distribution area. their spines constitute one of their defense mechanisms, which can be fomites for pathogens to humans. however, little is known about such pathogens. the present study aimed to detect bacteria on spines of c. subspinosus, from the una biological reserve, south of bahi ...201526436511
composition and bioactivities of an (e)-β-farnesene chemotype of chamomile (matricaria chamomilla) essential oil from nepal.the essential oil of matricaria chamomilla, collected from nepal, was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. the major components in nepalese chamomile oil were (e)-β-famesene (42.2%), α-bisabolol oxide a (22.3%), (e,e)-α-famesene (8.3%), cis-bicycloether (5.0%), α-bisabolol oxide b (4.5%), and α-bisabolone oxide a (4.0%). a cluster analysis based on the chemical compositions of 48 samples of chamomile oil reported in the literature has revealed seven ...201526434140
transcription factors wrky70 and wrky11 served as regulators in rhizobacterium bacillus cereus ar156-induced systemic resistance to pseudomonas syringae pv. tomato dc3000 in arabidopsis.the activation of both the sa and ja/etsignalling pathways may lead to more efficient general and broad resistance to pst dc3000 by non-pathogenic rhizobacteria. however, the mechanisms that govern this simultaneous activation are unclear. using arabidopsis as a model system, two transcription factors, wrky11 and wrky70, were identified as important regulators involved in induced systemic resistance (isr) triggered by bacillus cereus ar156. the results revealed that ar156 treatment significantly ...201626433201
single-step purification of chitosanases from bacillus cereus using expanded bed chromatography.a chitosanase-producing strain was isolated and identified as bacillus cereus c-01. the purification and characterization of two chitosanases were studied. the purification assay was accomplished by ion exchange expanded-bed chromatography. experiments were carried out in the presence and in the absence of cells through different expansion degree to evaluate the process performance. the adsorption experiments demonstrated that the biomass does not affect substantially the adsorption capacity of ...201626433174
identification of novel nitroreductases from bacillus cereus and their interaction with the cb1954 prodrug.directed enzyme prodrug therapy is a form of cancer chemotherapy in which bacterial prodrug-activating enzymes, or their encoding genes, are directed to the tumour before administration of a prodrug. the prodrug can then be activated into a toxic drug at the tumour site, reducing off-target effects. the bacterial nitroreductases are a class of enzymes used in this therapeutic approach and although very promising, the low turnover rate of prodrug by the most studied nitroreductase enzyme, nfnb fr ...201526415543
identification and characterization of bacillus cereus sw7-1 in bombyx mori (lepidoptera: bombycidae).the bacterial diseases of silkworms cause significant reductions in sericulture and result in huge economic loss. this study aimed to identify and characterize a pathogen from diseased silkworm. sw7-1, a pathogenic bacterial strain, was isolated from the diseased silkworm. the strain was identified on the basis of its bacteriological properties and 16s rrna gene sequence. the colony was round, slightly convex, opaque, dry, and milky on a nutrient agar medium, the colony also exhibited jagged edg ...201526411789
composition, in vitro cytotoxic, and antimicrobial activities of the flower essential oil of diospyros discolor from taiwan.this study investigated the chemical composition, in vitro cytotoxic, and antimicrobial activities of the essential oil isolated from the flower of diospyros discolor from taiwan. the essential oil was isolated using hydrodistillation in a clevenger-type apparatus, and characterized by gc-fid and gc-ms. twenty-eight compounds were identified, representing 100% of the oil. the main components identified were (2z,6e)-farnesol (35.0%), α-cadinol (10.9%), (e)-nerolidol (6.9%), α-humulene (6.0%), τ-c ...201526411038
isolation and characterization of bacteriocinogenic lactic bacteria from m-tuba and tepache, two traditional fermented beverages in méxico.mexican tuba (m-tuba) and tepache are mexican fermented beverages prepared mainly with pineapple pulp and coconut palm, respectively. at present, reports on the microbiota and nutritional effects of both beverages are lacking. the purpose of this study was to determine whether m-tuba and tepache contain cultivable lactic acid bacteria (lab) capable of producing bacteriocins. tepache and m-tuba contain mesophilic aerobic bacteria, lab, and yeast. bacillus subtilis, listeria monocytogenes, listeri ...201526405529
chemical composition and antimicrobial activity of hexane leaf extract of anisopus mannii (asclepiadaceae).the aim was to determine the chemical constituents and antimicrobial activity of the hexane leaf extract of anisopus mannii against a wide range of human pathogenic microorganisms.201526401399
antibacterial effect and mechanism of high-intensity 405 ± 5 nm light emitting diode on bacillus cereus, listeria monocytogenes, and staphylococcus aureus under refrigerated condition.this study investigated the antibacterial effect of 405 ± 5 nm light emitting diode (led) on bacillus cereus, listeria monocytogenes, and staphylococcus aureus, and examined its antibacterial mechanism by determining the bacterial membrane and dna damages. a 405 ± 5 nm led illuminated the gram-positive pathogens until 486 j/cm(2) at 4 °c. weibull model was used to calculate reliable life (tr) to compare bacterial sensitivities to led illumination. the membrane damage was determined by nacl and l ...201526398810
health-promoting value and food applications of black cumin essential oil: an overview.black cumin (nigella sativa l.) seeds and its essential oil have been widely used in functional foods, nutraceuticals and pharmaceutical products. analysis of nigella sativa essential oil using gc and gc-ms resulted in the identification of many bioactive compounds representing ca. 85 % of the total content. the main compounds included p-cymene, thymoquinone, α-thujene, longifolene, β-pinene, α-pinene and carvacrol. nigella sativa essential oil exhibited different biological activities including ...201526396361
complete genome sequence of bacillus thuringiensis strain hd521.bacillus thuringiensis is the most widely used biological pesticide in the world. it belongs to the bacillus cereus sensu lato group, which contains six species. among these six species, b. thuringiensis, b. anthracis, and b. cereus have a low genetic diversity. b. thuringiensis strain hd521 shows maroon colony which is different from most of the b. thuringiensis strains. strain hd521 also displays an ability to inhibit plant sheath blight disease pathogen (rhizoctonia solani ag1 ib) growth and ...201526380647
methylatable signaling helix coordinated inhibitory receiver domain in sensor kinase modulates environmental stress response in bacillus cereus.σb, an alternative transcription factor, controls the response of the cell to a variety of environmental stresses in bacillus cereus. previously, we reported that rsbm negatively regulates σb through the methylation of rsbk, a hybrid sensor kinase, on a signaling helix (s-helix). however, rsbk comprises a c-terminal receiver (rec) domain whose function remains unclear. in this study, deletion of the c-terminal rec domain of rsbk resulted in high constitutive σb expression independent of environm ...201526379238
genomic insights into the taxonomic status of the bacillus cereus group.the identification and phylogenetic relationships of bacteria within the bacillus cereus group are controversial. this study aimed at determining the taxonomic affiliations of these strains using the whole-genome sequence-based genome blast distance phylogeny (gbdp) approach. the gbdp analysis clearly separated 224 strains into 30 clusters, representing eleven known, partially merged species and accordingly 19-20 putative novel species. additionally, 16s rrna gene analysis, a novel variant of mu ...201526373441
molecular characterisation of bacillus chitinase for bioconversion of chitin waste.in this work chitin was extracted chemically from shrimp shells. seventeen bacillus isolates were screened for chitinolytic activity. the chitinolytic strains of bt. were screened at different temperatures and phs for their hydrolytic potentials. by using a pair of specific primers, endochitinase gene was amplified from sbs bt-5 strain through pcr, and then cloned into ptz57 ta cloning vector and transferred in escherichia coli dh5α strain. the sequenced gene (genbank accession no: he995800) con ...201626373305
structural and computational analysis of peptide recognition mechanism of class-c type penicillin binding protein, alkaline d-peptidase from bacillus cereus df4-b.alkaline d-peptidase from bacillus cereus df4-b, called adp, is a d-stereospecific endopeptidase reacting with oligopeptides containing d-phenylalanine (d-phe) at n-terminal penultimate residue. adp has attracted increasing attention because it is useful as a catalyst for synthesis of d-phe oligopeptides or, with the help of substrate mimetics, l-amino acid peptides and proteins. structure and functional analysis of adp is expected to elucidate molecular mechanism of adp. in this study, the crys ...201526370172
clinical characteristics and antimicrobial susceptibility of bacillus cereus blood stream infections.bacillus cereus is one of the pathogens causing nosocomial bloodstream infections (bsis). however, few reports have documented the antimicrobial susceptibility and clinical characteristics of bacillus cereus bsi and the importance of empirical therapy. the aim of this study was to investigate the clinical characteristics and antimicrobial susceptibility of b. cereus isolates from patients with bsi and to analyze the impact of appropriate empirical therapy on the outcome of patients with b. cereu ...201526370137
high-rate biological denitrification in the cyclic rotating-bed biological reactor: effect of cod/no3(-), nitrate concentration and salinity and the phylogenetic analysis of denitrifiers.the effects of cod/no3(-) ratio, nitrate concentration and salinity was tested on the performance of the crbr in denitrification with catechol as carbon source. the maximum nitrate reduction attained at cod/no3(-) ratio of 1. the crbr operated at optimum cod/no3(-) ratio could completely denitrify the nitrate at inlet concentration up to 1250mg/l without nitrite accumulation. the maximum denitrification rate in the crbr was 3.56kgno3(-)/m(3)d with a nitrate reduction efficiency of 99% when the b ...201526369277
merosesquiterpenoids and ten-membered macrolides from a soft coral-derived lophiostoma sp. fungus.one new merosesquiterpenoid, craterellin d (1), along with one known analog, craterellin a (2), and five known ten-membered macrolides, 3-7, were isolated from a soft coral-derived lophiostoma sp. fungus. the absolute configuration of 1 was established based on biogenetic consideration with the co-isolated analog 2, whose configuration was determined by modified mosher's method and single-crystal x-ray diffraction analysis using cukα radiation. the absolute configuration of 3 was determined by x ...201526363884
essential oils and diethyl ether extracts of serbian xeranthemum cylindraceum and x. annum: chemical composition, antimicrobial activity, and chemotaxonomic implications.detailed gc and gc-ms analyses of the essential oils and et2 o extracts of two xeranthemum species - x. cylindraceum and x. annum - resulted in the identification of 254 components, in total. terpenoids constituted the major part of both x. cylindraceum and x. annum essential oils and extracts (51.8-65.7%, and 50.7%, resp.). among the sesquiterpenoids, the extracts of both investigated taxa contained the guaianolide xerantholide, its 11,13-dihydro derivatives, and two additional sesquiterpene la ...201526363882
brown algae (phaeophyceae) from the coast of madagascar: preliminary bioactivity studies and isolation of natural products.eight species of brown algae (phaeophyceae) from the coast of madagascar have been investigated for their chemical constituents. fucosterol (3) was obtained as the most abundant compound. the brown alga sargassum ilicifolium was the source for the first isolation of the terpenoid c27-alcohol 1,1',2-trinorsqualenol (1) from marine sources. from s. incisifolium we isolated the highly unsaturated glycolipid 1-o-palmitoyl-2-o-stearidonoyl-3-o-β-d-galactopyranosylglycerol (4) and we report the first ...201526358714
antimicrobial susceptibility and the in vitro postantibiotic effects of vancomycin and ciprofloxacin against bacillus cereus isolates.vancomycin and ciprofloxacin were often used in the therapy of infections associated with bacillus cereus.201626358183
a cluster of cns infections due to b. cereus in the setting of acute myeloid leukemia: neuropathology in 5 patients.bacillus cereus typically causes a self-limited foodborne gastrointestinal (gi) illness. severe invasive infection occurs rarely, mainly among immunocompromised hosts. we describe a cluster of b. cereus infections among 5 patients with acute myeloid leukemia and chemotherapy-induced neutropenia. the initial case presented with occipital lobe abscess and was found on biopsy to have organisms consistent with bacillus species. within 1 week, a second patient died of fulminant brain swelling and hem ...201526352989
multiparametric quantitation of the bacillus cereus toxins cereulide and isocereulides a-g in foods.consumption of food products contaminated with cereulide (1), a toxin produced by bacillus cereus, might cause intoxications with symptoms reported to range from indigestion pain and emesis to death. recently, a series of structural variants, coined isocereulides a-g (2-8), were identified for the first time to be produced along with cereulide (1). the observation that isocereulide a (2) shows an ∼ 8-fold increased cytotoxicity when compared to 1 urges the development of analytical tools enablin ...201526352912
chemical composition and in vitro antibacterial activity of mentha spicata essential oil against common food-borne pathogenic bacteria.the aim of the present study was to investigate chemical composition and antibacterial activity of essential oil from the leaf of mentha spicata plant against common food-borne pathogenic bacteria (staphylococcus aureus, bacillus subtilis, bacillus cereus, listeria monocytogenes, salmonella typhimurium, and escherichia coli o157:h7). chemical composition of the essential oil was identified by gas chromatography coupled with mass spectrometer detector (gc-ms). the antibacterial activity of the es ...201526351584
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