Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| immunodiagnosis of ehrlichia canis infection with recombinant proteins. | ehrlichia canis causes a potentially fatal rickettsial disease of dogs that requires rapid and accurate diagnosis in order to initiate appropriate therapy leading to a favorable prognosis. we recently reported the cloning of two immunoreactive e. canis proteins, p28 and p140, that were applicable for serodiagnosis of the disease. in the present study we cloned a new immunoreactive e. canis surface protein gene of 1,170 bp, which encodes a protein with a predicted molecular mass of 42.6 kda (p43) ... | 2001 | 11136790 |
| coinfection with three ehrlichia species in dogs from thailand and venezuela with emphasis on consideration of 16s ribosomal dna secondary structure. | as part of a larger study to investigate tick-borne infections in dogs from thailand and venezuela, documentation of coinfection with three ehrlichia species in two dogs, one from each country, became the focus of the present study. although neither dog had clinical signs attributable to ehrlichiosis, both dogs were anemic and neutropenic and the thai dog was thrombocytopenic. genus- and species-specific pcr targeting the 16s rrna genes indicated that both dogs were coinfected with ehrlichia can ... | 2001 | 11136754 |
| enzyme-linked immunosorbent assays for detection of antibodies to ebola and marburg viruses using recombinant nucleoproteins. | the full-length nucleoprotein (np) of ebola virus (ebo) was expressed as a his-tagged recombinant protein (his-ebo-np) by a baculovirus system. carboxy-terminal halves of nps of ebo and marburg virus (mbg) were expressed as glutathione s-transferase-tagged recombinant proteins in an escherichia coli system. the antigenic regions on the nps of ebo and mbg were determined by both western blotting and enzyme-linked immunosorbent assay (elisa) to be located on the c-terminal halves. the c-terminal 1 ... | 2001 | 11136739 |
| hepatozoon canis infection associated with dog ticks of rural areas of rio de janeiro state, brazil. | hepatozoon canis is a tick-borne protozoan that infects dogs and has been reported throughout the world. manifestation of h. canis infection varies from being sub-clinical in apparently healthy dogs to severe illness. the main vector of the infection is the dog tick, rhipicephalus sanguineus although other species may also transmit this agent. h. canis has been reported previously in brazil, but mostly as an occasional finding during laboratory exams and always associated with other diseases. th ... | 2001 | 11113545 |
| canine ehrlichiosis caused simultaneously by ehrlichia canis and ehrlichia platys. | to identify the causative agent of canine ehrlichiosis that has occurred in the suburbs of guangzhou, china, since 1998, the 16s rrna gene was amplified and sequenced. two sequences of 1,482 and 1,483 base pairs were obtained and named as gzh981 and gzh982, respectively. the level of similarity of these two was 91.50%, and gzh981 closely resembled the 16s rrna gene of ehrlichia canis, whereas gzh982 resembled ehrlichia platys. we therefore conclude that e. canis and e. platys together caused rec ... | 2000 | 11092236 |
| infection of small ruminants with ehrlichia spp. in sicily. | in 1991, the experimental infection of a goat with pooled blood from goats that were positive for anti-ehrlichia canis, e. risticii, e. equi and e. phagocytophila antibodies was monitored (physical examination, cell blood count, microscopical examination of blood smears, serology) for 180 days. the infection produced a clinical condition characterized by intermittent fever, anaemia and leukopenia with neutropenia during the first 40 days. recurrent leukocytosis with lymphocytosis was noticed aft ... | 1999 | 11071551 |
| detection of platelet-bound antibodies in beagle dogs after artificial infection with ehrlichia canis. | six dogs were infected with ehrlichia canis by intravenous injection of heavily infected dh82 cells. all dogs developed typical signs of canine monocytic ehrlichiosis. using flow cytometric technology, platelet-bound igg (pbigg) were detected in 5 of the 6 dogs after experimental infection with e. canis over a period of 3-10 days post infection (pi). the first detection of pbigg was made as early as day 3 pi in 2 out of 6 dogs, and on day 5 pi in 1 dog. on day 7 pi, pbigg was detected in 2 dogs, ... | 2000 | 11068072 |
| monoclonal antibody binding to a surface-exposed epitope on cowdria ruminantium that is conserved among eight strains. | monoclonal antibodies (mab) binding to cowdria ruminantium elementary bodies (eb) were identified by enzyme-linked immunosorbent assay, and surface binding of one mab (446.15) to intact eb was determined by immunofluorescence, immunogold labeling, and transmission electron microscopy. mab 446.15 bound an antigen of approximately 43 kda in immunoblots of eight geographically distinct strains. the mab did not react with ehrlichia canis antigens or uninfected bovine endothelial cell lysate and may ... | 2000 | 11063511 |
| efficacy of a modified polymerase chain reaction assay for detection of ehrlichia canis infection. | detection of ehrlichia canis in acutely infected and convalescent dogs is important for effective treatment and control. however, accurate detection has been difficult to achieve, in part because dogs that have been treated therapeutically often remain seropositive for extended periods. a new method, polymerase chain reaction (pcr) assay using biotinylated e. canis-specific primers (pcr-bp), was developed for detection of e. canis. four dogs experimentally infected with e. canis by intravenous i ... | 2000 | 11021435 |
| a conserved, transcriptionally active p28 multigene locus of ehrlichia canis. | antigenic diversity of ehrlichia chaffeensis and ehrlichia canis may involve independent or differential expression of the p28 outer membrane proteins genes, enabling persistent infections of the natural hosts. in this study, we analyzed the transcriptional activity of a five gene locus in e. canis encoding homologous, but non-identical, p28 genes. the p28 multigene locus contained three previously identified complete gene sequences and one partial gene sequence. a new p28 gene was identified an ... | 2000 | 10974556 |
| cyclic cd8+ lymphopenia in dogs experimentally infected with bartonella vinsonii subsp. berkhoffii. | until recently, it was presumed that bartonella vinsonii only infected voles, a species of north american rodents. in april of 1993, however, our laboratory isolated a novel subspecies of b. vinsonii (b. vinsonii subsp. berkhoffii) from the blood of a dog diagnosed with vegetative valvular endocarditis. subsequently, based on a seroepidemiologic survey of dogs from north carolina and virginia presenting for a variety of medical problems, we found evidence supporting a potentially important assoc ... | 2000 | 10889298 |
| canine monocytic ehrlichiosis presenting as acute blindness 36 months after importation into the uk. | a four-year-old labrador retriever developed sudden-onset blindness, associated with bilateral uveitis, intraocular haemorrhage and retinal detachment. it had been imported into the uk from sardinia 36 months before presentation. haematological abnormalities included non-regenerative anaemia, thrombocytopenia and neutropenia. serum and urine protein electrophoresis demonstrated a monoclonal gammopathy. an immunofluorescent antibody test for ehrlichia canis was positive, with a titre of 1:320, co ... | 2000 | 10879405 |
| is the detection of anti-rhipicephalus sanguineus (rs24p) antibodies a valuable epidemiological tool of tick infestation in dogs? | antibodies against the 24 kda rhipicephalus sanguineus (rs24p) protein were detected by elisa to evaluate the relationship between antibodies and tick infestation. the mean titer of 3 dogs that underwent 2 experimental infestations with adult ticks was transiently increased after the second infestation. there was a significant difference in mean titers between positive control dogs naturally infested with ticks and tick-naive dogs. these results suggested that anti-rs24p antibodies detected by e ... | 2000 | 10863953 |
| comparison of a clinic-based elisa test kit with the immunofluorescence test for the assay of ehrlichia canis antibodies in dogs. | the "gold standard" for the detection of antibodies to ehrlichia canis, the cause of canine monocytic ehrlichiosis (cme), is the indirect immunofluorescence antibody (ifa) test. the ifa test however is generally available only in selected laboratories and requires extensive equipment and trained personnel. a double-blind study was conducted to compare the ability of an in-clinic standardized enzyme-linked immunosorbent assay (elisa) test kit to measure e. canis igg antibodies in dogs compared wi ... | 2000 | 10826837 |
| seroprevalence of ehrlichia canis, ehrlichia equi, and ehrlichia risticii in sick dogs from north carolina and virginia. | ehrlichia canis, e. equi, and e. risticii seroprevalence was determined by microimmunofluorescent antibody testing (ifa) in a sequential population of 1,845 sick dogs admitted during a 1-year period to the north carolina state university veterinary teaching hospital. a seroreactor was defined by a reciprocal ifa titer of > or =80 to e. canis, e. equi, or e. risticii antigens. of the 48 ifa seroreactors, 44 dogs were seroreactive to e. canis, 21 to e. equi, and 0 to e. risticii. seventeen dogs re ... | 2000 | 10668817 |
| molecular cloning and characterization of the 120-kilodalton protein gene of ehrlichia canis and application of the recombinant 120-kilodalton protein for serodiagnosis of canine ehrlichiosis. | the 120-kda outer membrane protein (p120) is a potential adhesin of ehrlichia chaffeensis, and recombinant p120 is very useful for serodiagnosis of human monocytotropic ehrlichiosis. the analogous gene of p120 in ehrlichia canis was cloned, sequenced, and expressed. like the e. chaffeensis p120, the e. canis p120 contains tandem repeat units. however, neither the repeat number nor the amino acid sequences in the repeats are identical in the two ehrlichia species. the repeat units are hydrophilic ... | 2000 | 10618118 |
| glycosylation of homologous immunodominant proteins of ehrlichia chaffeensis and ehrlichia canis. | the glycoprotein genes of ehrlichia chaffeensis (1,644 bp) and ehrlichia canis (2,064 bp) encode proteins of 548 to 688 amino acids with predicted molecular masses of only 61 and 73 kda but with electrophoretic mobilities of 120 kda (p120) and 140 kda (p140), respectively. the 120-kda protein gene of e. chaffeensis contains four identical 240-bp tandem repeat units, and the 140-kda protein gene of e. canis has 14 nearly identical, tandemly arranged 108-bp repeat units. conserved serine-rich moti ... | 2000 | 10603362 |
| serosurvey of ehrlichia canis and hepatozoon canis infection in dogs in yamaguchi prefecture, japan. | antibodies to ehrlichia canis and hepatozoon canis in dogs at the animal hospital in yamaguchi university were surveyed and potential risk factors for both pathogens were evaluated. among 430 dogs examined, 20 (4.7%) and 18 (4.2%) dogs showed positive findings for e. canis and h. canis, respectively. neither, sex nor age was associated with the seropositivity of either pathogen, but the positive rate in dogs kept outside was slightly higher than that in dogs kept inside for both pathogens. a hig ... | 1999 | 10563295 |
| emergence of anaplasma marginale antigenic variants during persistent rickettsemia. | anaplasma marginale is an ehrlichial pathogen of cattle, in the order rickettsiales, that establishes persistent cyclic rickettsemia in the infected host. within each rickettsemic cycle, a. marginale expressing antigenically variant major surface protein 2 (msp2) emerge. by cloning 17 full-length msp2 transcripts expressed during cyclic rickettsemia, we determined that emergent variants have a single, central hypervariable region encoding variant b-cell epitopes. the n- and c-terminal regions ar ... | 1999 | 10531237 |
| bartonella vinsonii subsp. berkhoffii and related members of the alpha subdivision of the proteobacteria in dogs with cardiac arrhythmias, endocarditis, or myocarditis. | cardiac arrhythmias, endocarditis, or myocarditis was identified in 12 dogs, of which 11 were seroreactive to bartonella vinsonii subspecies berkhoffii antigens. historical abnormalities were highly variable but frequently included substantial weight loss, syncope, collapse, or sudden death. fever was an infrequently detected abnormality. cardiac disease was diagnosed following an illness of short duration in most dogs, but a protracted illness of at least 6 months' duration was reported for fou ... | 1999 | 10523564 |
| efficacy of enrofloxacin for the treatment of experimentally induced ehrlichia canis infection. | 1999 | 10499737 | |
| variability in the 28-kda surface antigen protein multigene locus of isolates of the emerging disease agent ehrlichia chaffeensis suggests that it plays a role in immune evasion. | infections caused by rickettsial pathogens persist in vertebrate hosts for long periods of time, despite the active host immune response. we recently described the multigene locus encoding 28 kda surface antigen proteins (28 kda saps) for two closely related rickettsials, ehrlichia chaffeensis and ehrlichia canis (reddy, g. r., et al. (1998) biochem. biophys. res. commun. 247, 636-643), that share extensive structural homology to antigenic variant surface protein genes of neisseria and borrelia ... | 1999 | 10425222 |
| coinfection with multiple tick-borne pathogens in a walker hound kennel in north carolina. | both dogs and humans can be coinfected with various ehrlichia, bartonella, rickettsia, and babesia species. we investigated a kennel of sick walker hounds and their owners in southeastern north carolina for evidence of tick-borne infections and associated risk factors. a high degree of coinfection was documented in the dog population. of the 27 dogs, 26 were seroreactive to an ehrlichia sp., 16 to babesia canis, and 25 to bartonella vinsonii, and 22 seroconverted to rickettsia rickettsii antigen ... | 1999 | 10405413 |
| antibodies reactive with ehrlichia canis, ehrlichia phagocytophila genogroup antigens and the spotted fever group rickettsial antigens, in free-ranging jackals (canis aureus syriacus) from israel. | a seroepidemiological survey was conducted to investigate the prevalence of antibodies reactive with the ehrlichia canis and ehrlichia phagocytophila genogroup antigens, and the spotted fever group (sfg) rickettsiae antigens in jackals in israel (canis aureus syriacus), to assess the possible role of the jackal in the epidemiology of these diseases. fifty-three serum samples from jackals were assayed by the indirect immunofluorescence antibody test. antibodies to e. canis were detected in 35.8% ... | 1999 | 10321583 |
| molecular cloning of the gene for a conserved major immunoreactive 28-kilodalton protein of ehrlichia canis: a potential serodiagnostic antigen. | a gene encoding a 28-kda protein of ehrlichia canis was cloned, sequenced, and expressed, and a comparative molecular analysis with homologous genes of e. canis, cowdria ruminantium, and ehrlichia chaffeensis was performed. the complete gene has an 834-bp open reading frame encoding a protein of 278 amino acids with a predicted molecular mass of 30.5 kda. an n-terminal signal sequence was identified, suggesting that the protein undergoes posttranslational modification to a mature 27.7-kda protei ... | 1999 | 10225842 |
| the map1 gene of cowdria ruminantium is a member of a multigene family containing both conserved and variable genes. | heartwater is an economically important disease of ruminants caused by the tick-transmitted rickettsia cowdria ruminantium. the disease is present in africa and the caribbean and there is a risk of spread to the americas, particularly because of a clinically asymptomatic carrier state in infected livestock and imported wild animals. the causative agent is closely related taxonomically to the human and animal pathogens ehrlichia chaffeensis and ehrlichia canis. a dominant immune response of infec ... | 1999 | 10198207 |
| serological survey for antibodies reactive with ehrlichia canis and e. chaffeensis in dogs from the bloemfontein area, south africa. | sera from 161 dogs in the bloemfontein area in south africa were tested for the presence of antibodies reactive with ehrlichia canis and e. chaffeensis by indirect fluorescent antibody testing. overall, 68 (42%) of the dogs had significant antibody titres (> or = 1/64) against e. canis and 61 (38%) had significant titres (> or = 1/64) against e. chaffeensis. seven (11%) dogs had higher titres to e. chaffeensis than e. canis (1/2048 and 1/1024 (2 dogs); 1/1024 and 1/512 (2 dogs); 1/2048 and 1/512 ... | 1998 | 10192085 |
| serological evidence of infection with ehrlichia spp. in red foxes (vulpes vulpes) in switzerland. | serum samples from 1,550 red foxes in switzerland were tested for antibodies to the agents of canine granulocytic and monocytic ehrlichiosis by an indirect immunofluorescent technique. forty-four (2.8%) of the samples were positive for ehrlichia phagocytophila, which is an antigen marker for granulocytic ehrlichiosis. in contrast, none of the samples had antibodies specific to ehrlichia canis, the agent of monocytic ehrlichiosis. | 1999 | 10074544 |
| potential value of major antigenic protein 2 for serological diagnosis of heartwater and related ehrlichial infections. | cowdria ruminantium is the etiologic agent of heartwater, a disease causing major economic loss in ruminants in sub-saharan africa and the caribbean. development of a serodiagnostic test is essential for determining the carrier status of animals from regions where heartwater is endemic, but most available tests give false-positive reactions with sera against related erhlichia species. current approaches rely on molecular methods to define proteins and epitopes that may allow specific diagnosis. ... | 1999 | 10066656 |
| platelet-associated immunoglobulin (antiplatelet antibody) in canine rocky mountain spotted fever and ehrlichiosis. | antiplatelet antibodies were detected in the sera of dogs with naturally occurring and experimentally induced rickettsia rickettsii and ehrlichia canis infections. this is the first known report documenting elevated platelet-associated immunoglobulin (paig) titers in rocky mountain spotted fever (rmsf) infections. in the naturally occurring rmsf infections and ehrlichiosis, the antibodies persisted for weeks or months, even when the platelet counts had normalized. results of this study indicate ... | 1999 | 9934930 |
| kinetics of serologic cross-reactions between ehrlichia canis and the ehrlichia phagocytophila genogroups in experimental e. canis infection in dogs. | the serological cross-reactions between the ehrlichia canis and ehrlichia phagocytophila genogroups, and the kinetics of development of antibodies in dogs to the e. phagocytophila group after artificial infection with the israeli strain of e. canis was investigated. results of this study indicate that the development of antibodies to the e. phagocytophila genogroup in dogs after infection with e. canis is a time dependent event probably conditional on the continued propagation of the rickettsia ... | 1998 | 9880101 |
| [ehrlichia canis infections of dogs in germany. epidemiology, diagnosis, therapy and prophylaxis]. | between january and december 1997 infections with ehrlichia canis were detected in 211 dogs in germany. of the 53 epidemiologically evaluable dogs, 19 animals born and raised in germany had travelled with their owners abroad in endemic areas, 30 dogs originated from there and four dogs had never left germany. as regards to the possible location of infection it has been registered that most dogs had been taken to countries of the mediterranean sea (spain, france, italy, greece) or had been import ... | 1998 | 9857421 |
| a molecular and serologic survey of ehrlichia canis, e. chaffeensis, and e. ewingii in dogs and ticks from oklahoma. | polymerase chain reaction and southern hybridization were used to survey for the presence of ehrlichia canis, ehrlichia chaffeensis, and ehrlichia ewingii in blood samples of 65 dogs that harbored ticks from northcentral and northeastern oklahoma. dog blood samples were also examined for antibodies against e. canis and e. chaffeensis, using an immunofluorescent antibody test. ten of 65 dogs (15.4%) examined were positive for ehrlichia spp. by pcr. four (6.2%) were positive for e. ewingii, 2 (3.1 ... | 1998 | 9831955 |
| seroprevalence of ehrlichia canis and of canine granulocytic ehrlichia infection in dogs in switzerland. | serum samples from 996 dogs in switzerland were examined for antibodies to ehrlichia canis and to the agent causing canine granulocytic ehrlichiosis (cge). ehrlichiosis, borreliosis, and systemic illness not associated with ticks were suspected in 75, 122, and 157 of these dogs, respectively. the remainder of the serum samples were obtained from clinically healthy dogs which resided north (n = 235) or south (n = 407) of the alps. the serum samples were tested by an indirect immunofluorescence te ... | 1998 | 9817854 |
| characterization of monoclonal antibodies to the 44-kilodalton major outer membrane protein of the human granulocytic ehrlichiosis agent. | the major outer membrane proteins (omps) of the human granulocytic ehrlichiosis (hge) agent, with molecular sizes of 44 to 47 kda, are immunodominant antigens in human infection. monoclonal antibodies (mabs) to the omps were made by immunizing balb/c mice with the purified hge agent and then by fusing spleen cells with myeloma cells. the immunologic specificities of three mabs (3e65, 5c11, and 5d13) were examined with five human hge agent isolates and one tick isolate. by western blot analysis, ... | 1998 | 9774579 |
| lack of susceptibility of ehrlichia canis to imidocarb dipropionate in vitro. | in vitro antimicrobial susceptibility testing was used to compare the efficacy of imidocarb dipropionate and doxycycline on the growth of ehrlichia canis in dh82 cell cultures. over a 9-day period there were no significant differences (p < 0.01) in the growth of e. canis in untreated control wells and those to which imidocarb dipropionate was added at 1.2, 2.4, 4.8 or 12 micrograms/ml for the 1st 3 days. average infection rates rose from 50 to 55% on day 0 to 100% on day 5 or 6. doxycycline at 1 ... | 1998 | 9760397 |
| acute blindness associated with monoclonal gammopathy induced by ehrlichia canis infection. | ehrlichia canis infection was diagnosed in a labrador retriever presented with a primary complaint of acute blindness. ocular signs on admission included bilateral hyphema, retinal haemorrhage and retinal detachment. serum protein electrophoresis results revealed monoclonal gammopathy. this report discusses and suggests the pathogenesis of ocular bleeding in canine monocytic ehrlichiosis. blood hyperviscosity, elevation in oncotic pressure, vasculitis, thrombocytopenia and platelet dysfunction a ... | 1998 | 9735920 |
| cloning and characterization of multigenes encoding the immunodominant 30-kilodalton major outer membrane proteins of ehrlichia canis and application of the recombinant protein for serodiagnosis. | a 30-kda major outer membrane protein of ehrlichia canis, the agent of canine ehrlichiosis, is the major antigen recognized by both naturally and experimentally infected dog sera. the protein cross-reacts with a serum against a recombinant 28-kda protein (rp28), one of the outer membrane proteins of a gene (omp-1) family of ehrlichia chaffeensis. two dna fragments of e. canis were amplified by pcr with two primer pairs based on the sequences of e. chaffeensis omp-1 genes, cloned, and sequenced. ... | 1998 | 9705412 |
| sequential evaluation of dogs naturally infected with ehrlichia canis, ehrlichia chaffeensis, ehrlichia equi, ehrlichia ewingii, or bartonella vinsonii. | historically, disease manifestations in dogs seroreactive to ehrlichia canis antigens by indirect immunofluorescent antibody testing have been attributed to infection with either e. canis or ehrlichia ewingii. a 1996 study by dawson and colleagues provided pcr evidence that healthy dogs from southeastern virginia could be naturally infected with ehrlichia chaffeensis. this observation stimulated us to determine which ehrlichia spp. infected sick dogs that were referred to our hospital from the s ... | 1998 | 9705408 |
| survey of tick-borne diseases in dogs infested with rhipicephalus sanguineus at a kennel in okayama prefecture, japan. | serological tests were performed to investigate extent of tick-borne diseases in dogs infested with rhipicephalus sanguineus at a kennel in okayama prefecture. three of 22 dogs (13.6%) were positive for ehrlichia canis. two of 19 dogs (10.5%) were positive for rickettsia japonica. three of 22 dogs (13.6%) were positive for babesia gibsoni. none of these animals were positive for coxiella burnetii or hepatozoon canis. a microfilaria was detected in a drop smear of hemolymph from an engorged femal ... | 1998 | 9673953 |
| therapeutic effect of doxycycline in experimental subclinical canine monocytic ehrlichiosis: evaluation of a 6-week course. | the efficacy of doxycycline treatment (10 mg/kg of body weight every 24 h for 42 days) in eliminating ehrlichia canis from four subclinically infected dogs was evaluated. one dog remained pcr positive, suggesting that 6 weeks of doxycycline treatment may not be sufficient to clear e. canis parasites from all subclinically infected dogs. serology (indirect immunofluorescent antibody assay) was shown to be unreliable in assessing recovery from the carrier state, as anti-e. canis antibodies persist ... | 1998 | 9650986 |
| molecular characterization of a 28 kda surface antigen gene family of the tribe ehrlichiae. | antisera against different ehrlichiae recognize an immunodominant, cross-reacting approximately 28 kda surface antigen defined as the map1 in cowdria ruminantium. these antigens are considered valuable in developing serodiagnostic tests and recombinant vaccines for ehrlichiae infections. to evaluate the relationship in three closely related ehrlichiae, ehrlichia chaffeensis, ehrlichia canis, and c. ruminantium, the structure of the 28 kda antigen genes was analyzed. we describe the cloning and c ... | 1998 | 9647746 |
| investigation of splenic functions in canine monocytic ehrlichiosis. | in order to determine the role of the spleen in the pathogenesis of canine monocytic ehrlichiosis (cme), the effect of splenectomy on the course of the acute phase of experimental was investigated. intact and splenectomized dogs, sero-negative for ehrlichia canis antibodies, were infected with the israeli strain of e. canis. serology, clinical signs and haematological parameters were recorded prior to infection, and over a period of 60 days post infection, and were compared between the intact an ... | 1998 | 9618865 |
| antibody response to hepatozoon canis in experimentally infected dogs. | canine hepatozoonosis is a disease caused by the tick-borne protozoan hepatozoon canis. five puppies were inoculated by ingestion of rhipicephalus sanguineus ticks experimentally infected with h. canis, and all became infected with h. canis: gametocytes were detected in blood smears from four dogs and schizonts were observed in the spleen and bone marrow of the fifth. antibodies reactive with h. canis gametocytes were detected by the indirect fluorescent antibody test (ifa), with igm detected in ... | 1998 | 9561714 |
| experimental transmission of ehrlichia canis (rickettsiales: ehrlichieae) by dermacentor variabilis (acari: ixodidae). | four trials were conducted in which laboratory-reared dermacentor variabilis nymphs were exposed to ehrlichia canis by feeding on experimentally infected dogs as soon as classical morulae were detected in peripheral blood monocytes. after molting 25, 50 or 90 adult tick pairs were permitted to feed on 7 ehrlichia-naive dogs. transmission occurred in trials 1 (1/1 dog), 3 (1/1 dog) and 4 (2/2 dogs) but not in trial 2 (0/3 dogs), with 4 of 7 dogs becoming infected. successful transstadial transmis ... | 1998 | 9561712 |
| a survey of tick-borne bacteria and protozoa in naturally exposed dogs from israel. | antibody reactivity against seven bacterial or protozoal pathogens was measured in sera derived from 40 dogs suspected of a tick-borne disease. sera from 73% (29/40) of the dogs reacted with three or more test antigens. seroreactivity was most prevalent to babesia canis antigen (90%) followed by babesia gibsoni (75%), ehrlichia canis (63%), rickettsia conorii--moroccan strain (58%), rickettsia conorii--israeli strain no. 2 (28%), borrelia burgdorferi (10%) or bartonella vinsonii (berkhoffii) (10 ... | 1998 | 9561701 |
| granulocytic ehrlichiosis in dogs from north carolina and virginia. | medical records of 3 dogs from north carolina and 3 dogs from virginia with ehrlichial morulae in circulating neutrophils were studied retrospectively. two clinically distinct disease syndromes, including chronic, moderate to severe anemia (n = 3) and polyarthritis (n = 2) were associated with canine granulocytic ehrlichiosis (cge) in these dogs. one dog was clinically healthy, and abnormalities were not detected during physical examination. clinical signs were nonspecific and included fever, le ... | 1998 | 9560760 |
| [imported arthropod-borne parasites and parasitic arthropods in dogs. species spectrum and epidemiologic analysis of the cases diagnosed in 1995/96]. | between january 1995 and december 1996 nonendemic or only regionally occurring arthropodborne parasites including ehrlichiae and parasitic arthropods in germany were detected in 484 dogs, whereby at least 15 species were involved. listed in decreasing order, leishmania infections occurred most frequently, followed by infections/infestations with babesia canis, ehrlichia canis, rhipicephalus sanguineus, dirofilaria immitis and dermacentor reticulatus. the other species, namely babesia gibsoni, tr ... | 1998 | 9531673 |
| doxycycline hyclate treatment of experimental canine ehrlichiosis followed by challenge inoculation with two ehrlichia canis strains. | dogs were experimentally inoculated with ehrlichia canis florida to assess the efficacy of doxycycline hyclate for the treatment of acute ehrlichiosis. treatment with doxycycline eliminated infection in eight of eight dogs. untreated infected control dogs appeared to eliminate the infection or, alternatively, suppress the degree of ehrlichiemia to a level not detectable by tissue culture isolation or pcr or by transfusion of blood into recipient dogs. prior infection did not infer protection aga ... | 1998 | 9527787 |
| amplification of ehrlichial dna from dogs 34 months after infection with ehrlichia canis. | in order to determine whether dogs in the subclinical phase of canine monocytic ehrlichiosis (cme) are carriers of ehrlichia canis and to determine the significance of persistent indirect immunofluorescent anti-e. canis antibody titers during this phase, pcr was performed with blood, bone marrow, and splenic aspirates collected 34 months postinoculation from six clinically healthy beagle dogs experimentally infected with e. canis. at least one of the three samples (spleen, bone marrow, and blood ... | 1998 | 9431923 |
| immunodominant major outer membrane proteins of ehrlichia chaffeensis are encoded by a polymorphic multigene family. | several immunodominant major proteins ranging from 23 to 30 kda were identified in the outer membrane fractions of ehrlichia chaffeensis and ehrlichia canis. the n-terminal amino acid sequence of a 28-kda protein of e. chaffeensis (one of the major proteins) was determined. the gene (p28), almost full length, encoding the 28-kda protein was cloned by pcr with primers designed based on the n-terminal sequence of the e. chaffeensis 28-kda protein and the consensus sequence between the c termini of ... | 1998 | 9423849 |
| western immunoblotting analysis of the antibody responses of patients with human monocytotropic ehrlichiosis to different strains of ehrlichia chaffeensis and ehrlichia canis. | in order to evaluate the relative sensitivity of the detection of antibodies against various antigenic proteins of ehrlichia chaffeensis for the diagnosis of the emerging infectious disease human monocytotropic ehrlichiosis, western immunoblotting was performed with 27 serum samples from convalescent patients with antibodies, as demonstrated by indirect immunofluorescence assay. among 22 patients with antibodies reactive with the 120-kda protein, 15 showed reactivity with the 29/28-kda protein(s ... | 1997 | 9384299 |
| canine monocytic ehrlichiosis: a retrospective study of 100 cases, and an epidemiological investigation of prognostic indicators for the disease. | one hundred cases of monocytic ehrlichiosis diagnosed in israeli dogs were confirmed by the presence of anti-ehrlichia canis indirect immunofluorescent antibody titres greater than 1:40. the disease occurred in all age groups and there was no sex predilection. german shepherd dogs were significantly over-represented whereas crossbreed dogs were significantly under-represented (p > 0.0005). the most common clinical signs were depression, lethargy, lymphadenomegaly, fever, anorexia, panting, pale ... | 1997 | 9351183 |
| thrombocytopathia and light-chain proteinuria in a dog naturally infected with ehrlichia canis. | a 6-year-old dog was presented for evaluation of recurrent epistaxis. platelet counts, biochemical tests, and coagulation tests were within the normal range, but a mucosal bleeding time was prolonged; there was hyperproteinemia and a monoclonal gammopathy. heterogeneity of light chains appeared in urine, however, thus suggesting that the gammopathy was polyclonal. platelet aggregation tests showed decreased responsiveness to collagen. an ehrlichia canis indirect fluorescent-antibody titer was po ... | 1997 | 9348500 |
| different organisms associated with heartwater as shown by analysis of 16s ribosomal rna gene sequences. | cowdria ruminantium is a rickettsial parasite which causes heartwater, a economically important disease of domestic and wild ruminants in tropical and subtropical africa and parts of the caribbean. because existing diagnostic methods are unreliable, we investigated the small-subunit ribosomal rna (srrna) gene from heartwater-infected material to characterise the organisms present and to develop specific oligonucleotide probes for polymerase chain reaction (pcr) based diagnosis. dna was obtained ... | 1997 | 9299697 |
| [hepatozoon canis infection of dogs in germany: case report and epidemiology]. | this paper reports on a case of hepatozoonosis of a dog imported from italy to germany being additionally infected with ehrlichia canis and babesia canis. furthermore, a survey of the epidemiology of the hepatozoon canis infection is presented. | 1997 | 9289886 |
| pcr amplification and comparison of nucleotide sequences from the groesl heat shock operon of ehrlichia species. | degenerate pcr primers derived from conserved regions of the eubacterial groesl heat shock operon were used to amplify groesl sequences of ehrlichia equi, ehrlichia phagocytophila, the agent of human granulocytic ehrlichiosis (hge), ehrlichia canis, bartonella henselae, and rickettsia rickettsii. the groesl nucleotide sequences were less conserved than the previously determined 16s rrna gene sequences of these bacteria. a phylogenetic tree derived from deduced groel amino acid sequences was simi ... | 1997 | 9230387 |
| development and evaluation of a recombinant antigen, monoclonal antibody-based competitive elisa for heartwater serodiagnosis. | cowdria ruminantium is the etiologic agent of heartwater, a tick-transmitted foreign animal disease with considerable potential for entrance into the usa. a competitive enzyme-linked immunosorbent assay (celisa) was developed to detect serologic responses to c. ruminantium infection. the celisa utilized a recombinant form of the c. ruminantium major antigenic protein (map-1) as the antigen and an anti-map-1 monoclonal antibody as the competing indicator reagent. experimental antisera to c. rumin ... | 1997 | 9211230 |
| comparison of nested pcr with immunofluorescent-antibody assay for detection of ehrlichia canis infection in dogs treated with doxycycline. | a partial 16s rrna gene was amplified in ehrlichia canis-infected cells by nested pcr. the assay was specific and did not amplify the closely related ehrlichia chaffeensis, ehrlichia muris, neorickettsia helminthoeca, and sf agent 16s rrna genes. the assay was as sensitive as southern hybridization, detecting as little as 0.2 pg of e. canis dna. by this method, all blood samples from four dogs experimentally infected with e. canis were positive as early as day 4 postinoculation, which was before ... | 1997 | 9196207 |
| characterization of the subclinical phase of canine ehrlichiosis in experimentally infected beagle dogs. | beagle dogs were examined during the subclinical phase of canine ehrlichiosis under controlled conditions. emphasis was placed on gathering data before artificial inoculation with ehrlichia canis, and comparing these data with those of the subclinical phase of the disease. in this study all dogs were clinically healthy throughout the 6 month examination period. all subclinically infected dogs had ifa antibody titers to e. canis at a dilution varying from 1:2560 to 1:20480. the most prominent hae ... | 1997 | 9195740 |
| application of the polymerase chain reaction for the detection of ehrlichia canis in tissues of dogs. | doxycycline treatment resulted in a carrier status in 3 dogs and complete clearance of ehrlichia canis in 2 dogs (iqbal and rikihisa, 1994). using specimens obtained during that study applicability of polymerase chain reactions (pcrs) in detecting e. canis dna in tissue specimens and correlation of pcr results with our previous cell culture isolation results were evaluated. pcrs using a pair of primers specific to e. canis 16srrna gene sequence were used to detect dna of e. canis in tissues of 5 ... | 1994 | 9133053 |
| immunoblot analysis of the immunoglobulin g response to ehrlichia canis in dogs: an international survey. | historically, considerable variation has been reported in the type and severity of clinical and hematologic abnormalities associated with canine ehrlichiosis. because of difficulties associated with the isolation of intracellular monocytic ehrlichia species in tissue culture systems, few e. canis isolates are available for comparative microbiologic studies. to address the issue of potential e. canis antigenic diversity in different regions of the world, dog sera reactive by indirect fluorescent ... | 1997 | 9087922 |
| clinical and clinicopathologic abnormalities in greyhounds with cutaneous and renal glomerular vasculopathy: 18 cases (1992-1994) | to determine clinical signs and clinicopathologic abnormalities in greyhounds with cutaneous and renal glomerular vasculopathy and to determine whether there were any differences between dogs with and without renal azotemia. | 1997 | 9074681 |
| suspected ehrlichial infection in five cats from a household. | ehrlichiosis is a poorly recognized condition of cats that may be associated with anemia, leukopenia, thrombocytopenia, or dysproteinemia. affected cats may have indirect fluorescent antibody titers to ehrlichia canis and e risticii. we reviewed the clinical evaluation and response to treatment of 5 cats in a household where ehrlichial disease was suspected as the cause of recurrent leukopenias and thrombocytopenias. all of the cats had e risticii indirect fluorescent antibody titers and western ... | 1997 | 9018358 |
| serum protein alterations in canine ehrlichiosis. | serum protein electrophoresis was performed in 42 dogs with naturally occurring ehrlichia canis infection and in 15 clinically healthy dogs (control dogs). the infected dogs were found to have a significant hypoalbuminaemia, hyperglobulinaemia and hypergammaglobulinaemia compared to the control dogs (p < 0.001). a polyclonal gammopathy was found in all but one of the infected dogs which presented a monoclonal gammopathy. alpha-1 globulin was lower while alpha-2 and beta-2 globulin concentrations ... | 1996 | 9017886 |
| detection of ehrlichial antigen in plasma of beagle dogs with experimental acute ehrlichia canis infection. | six beagles were experimentally infected with ehrlichia canis. all dogs developed typical clinical signs of ehrlichiosis and sero-converted. ehrlichial antigenemia in the plasma of the infected dogs was detected using a sandwich enzyme-linked immunosorbent assay (elisa). ehrlichial antigen was present starting 15-20 days post-infection, after the development of clinical signs and antibody titre to ehrlichia canis. the appearance of ehrlichial antigen in the plasma for a relatively short and vari ... | 1996 | 8966999 |
| pcr detection of acute ehrlichia canis infection in dogs. | a polymerase chain reaction (pcr)-based detection assay that specifically detected ehrlichia canis in dogs with acute infections was developed. a region of the 16s ribosomal rna gene of e. canis was targeted for pcr amplification and chemiluminescent hybridization (ch) with a complementary internal 287-base pair (bp) oligonucleotide probe. the ch improved the pcr assay sensitivity 1,000-fold as compared with visualization on ethidium bromide-stained agarose gels. the pcr assay with ch (pcr/ch) d ... | 1996 | 8953528 |
| specific amplification of ehrlichia platys dna from blood specimens by two-step pcr. | a two-step pcr method for diagnosis of canine infectious cyclic thrombocytopenia was established. three primers derived from the 16s and rrna gene sequence were used to amplify genomic dna specifically from ehrlichia platys. two-round amplification with dna templates prepared from e. platys-infected blood specimens produced 742- and 385-bp fragments, but these products were not found when an ehrlichia canis-infected blood sample and escherichia coli were used. this method, for which the minimum ... | 1996 | 8940461 |
| attempted transmission of ehrlichia canis by rhipicephalus sanguineus after passage in cell culture. | to compare the transmissibility by the brown dog tick, rhipicephalus sanguineus, of a recent isolate of ehrlichia canis (ebony) with that of another isolate (oklahoma) that had been passaged in cell culture, and to assess the genetic similarity of the 2 isolates as reflected in the nucleotide (nt) sequence of 16s rdna. | 1996 | 8915436 |
| platelet dysfunction associated with experimental acute canine ehrlichiosis. | to determine whether platelet dysfunction occurs in canine ehrlichiosis, platelet aggregation studies in response to collagen/epinephrine, thrombin and adenosine diphosphate (adp) were carried out by the indirect method, using sera from six dogs experimentally infected with ehrlichia canis. samples of serum taken before infection and four and 20 days after infection were tested by incubation with platelet-rich plasma from a seronegative healthy dog. platelet aggregation was significantly inhibit ... | 1996 | 8890464 |
| ehrlichia canis-like agent isolated from a man in venezuela: antigenic and genetic characterization. | we report the first isolation and molecular and antigenic characterization of a human ehrlichial species in south america. a retrospective study was performed with serum specimens from 6 children with clinical signs suggestive of human ehrlichiosis and 43 apparently healthy adults who had a close contact with dogs exhibiting clinical signs compatible with canine ehrlichiosis. the evaluation was performed by the indirect fluorescent-antibody assay with ehrlichia chaffeensis arkansas, ehrlichia ca ... | 1996 | 8862572 |
| specific, nonradioactive detection of the nhp bacterium in penaeus vannamei by in situ hybridization. | necrotizing hepatopancreatitis (nhp) is a disease of farm-raised pacific white shrimp (penaeus vannamei) caused by a pleomorphic intracellular bacterium. a dna probe that is specific for the etiologic agent of necrotizing hepatopancreatitis was devised and tested in an in situ hybridization assay. a procedure was developed for labeling a single-stranded dna probe with digoxigenin by the polymerase chain reaction. the dna probe encompasses the v1 and v2 variable regions of the 16s ribosomal rna ( ... | 1996 | 8844575 |
| a recombinant antigen from the heartwater agent (cowdria ruminatium) reactive with antibodies in some southeastern united states white-tailed deer (odocoileus virginianus), but not cattle, sera. | recombinant baculovirus techniques were used to express the 260 amino acid carboxyterminal portion of the 32 kilodalton (kda) major antigenic protein (map 1) of cowdria ruminantium, the heartwater agent, as a fusion protein. the recombinant map 1 was fused to an aminoterminal independently antigenic octapeptide sequence (flag peptide). recombinant map 1 was used as an immunoblotting antigen to evaluate numerous reference antisera against organisms of the tribe ehrlichieae. monoclonal and polyclo ... | 1996 | 8827667 |
| post-therapy antibody titers in dogs with ehrlichiosis: follow-up study on 68 patients treated primarily with tetracycline and/or doxycycline. | the clinical and serological responses to therapy were evaluated for at least 1 year in 68 dogs with antibody titers positive for ehrlichia canis. treatments were of variable periods with primarily tetracycline hydorchloride and/or doxycycline. sixteen dogs had initial titers of 1:20 and, at the end of the year, were asymptomatic, no longer receiving medication, and had negative serology. the average length of treatment with tetracycline hcl and/or doxycycline was 85 days (range, 14 to 360 days) ... | 1996 | 8819054 |
| kinetics of serum antiplatelet antibodies in experimental acute canine ehrlichiosis. | the pattern of appearance of serum antiplatelet antibodies during the acute phase of experimental canine ehrlichiosis (ehrlichia canis) was investigated in six beagles and correlated with the development of thrombocytopenia. the earliest detection of serum antiplatelet antibodies was made on day 7 post-inoculation in one dog, on day 13 in three out of six dogs, and on day 17 post-inoculation in the remaining two dogs. thrombocytopenia developed in all infected dogs. the results of this study sug ... | 1996 | 8797272 |
| recombinant expression and use in serology of a specific fragment from the cowdria ruminantium map1 protein. | the major antigenic protein (map1) of cowdria ruminantium was screened for immunogenic regions by expression of overlapping recombinant dna clones of the gene encoding the map1 protein. two regions, designated map1-a and map1-b, were recognized by all antisera to 9 different isolates of c. ruminantium. map1-a contained one or more epitopes responsible for false-positive reactions with ehrlichia antisera in several serological tests for cowdriosis. cross-reactivity with map1-b was limited to anti ... | 1996 | 8784484 |
| [the brown dog tick, rhipicephalus sanguineus (ixodidae), in germany: an epidemiologic study and control measures]. | between january and december 1995 infestations with rhipicephalus sanguineus were established in 22 dogs in germany. out of these 22 animals 2 originated from the endemic area of this tick species, spain and the dominican republic and 11 dogs accompanied their owner to the mediterranean countries, 5 to italy, 3 to france, 2 to spain and 1 animal to greece. six dogs had never left germany and with 3 dogs the way of infestation could not be reconstructed. in 16 cases an abundant occurrence of r. s ... | 1996 | 8765542 |
| geographic, clinical, serologic, and molecular evidence of granulocytic ehrlichiosis, a likely zoonotic disease, in minnesota and wisconsin dogs. | seventeen minnesota and wisconsin dogs with granulocytic ehrlichosis were studied. the diagnoses were made by finding ehrlichia morulae in peripheral blood neutrophils. eight dogs were studied retrospectively, and nine dogs were studied prospectively. the medical records of all dogs were reviewed. eighty-eight percent of the dogs were purebred and 76% were spayed females. the median age was 8 years. sixty-five percent of the cases were diagnosed in october and november. fever and lethargy were t ... | 1996 | 8748270 |
| antibodies in cat sera from southern africa react with antigens of ehrlichia canis. | 1996 | 8737264 | |
| isolation of ehrlichia canis from dogs following subcutaneous inoculation. | subcutaneous inoculation of dogs with ehrlichia canis was investigated as a more appropriate model of canine ehrlichiosis, which is naturally transmitted by arthropod vectors. a dose-dependent response occurred following subcutaneous inoculation of seven groups of dogs with log concentrations of e. canis-infected canine-origin cells. ehrlichial infection in dogs was defined as concurrence of an increased titer of anti-e. canis immunoglobulin g (igg) antibody in serum, a decreased platelet concen ... | 1996 | 8735093 |
| survey of ehrlichia canis antibodies among dogs in israel. | a seroepidemiological survey was made of the prevalence of antibodies reactive with ehrlichia canis in stray and pet dogs from five geographical regions of israel. serum samples from 410 dogs were assayed by an indirect fluorescent antibody test and serum reactive at a 1:20 dilution or greater was considered positive. the overall seroprevalence was 30 per cent with 23.9 per cent in pet dogs and 37.5 per cent in stray dogs. the seroprevalence in apparently healthy pets (17.6 per cent) was lower b ... | 1996 | 8734508 |
| the first isolation, in vitro propagation, and genetic characterization of ehrlichia canis in israel. | ehrlichia canis, the etiologic agent of canine ehrlichiosis, was isolated in israel from a naturally infected dog with acute signs of the disease. the organism designated e. canis 611, was passaged experimentally to a beagle, from which it was propagated in primary canine monocytes. the organism was then grown in vitro in a continuous canine cell line, dh82. nine beagles subsequently injected with whole e. canis-infected blood all developed typical symptoms of ehrlichiosis. an indirect immunoflu ... | 1996 | 8686178 |
| hemocytic rickettsia-like organisms in ticks: serologic reactivity with antisera to ehrlichiae and detection of dna of agent of human granulocytic ehrlichiosis by pcr. | ixodid ticks were collected from connecticut, massachusetts, missouri, pennsylvania, rhode island, and british columbia (canada) during 1991 to 1994 to determine the prevalence of infection with hemocytic (blood cell), rickettsia-like organisms. hemolymph obtained from these ticks was analyzed by direct and indirect fluorescent antibody (fa) staining methods with dog, horse, or human sera containing antibodies to ehrlichia canis, ehrlichia equi, or rickettsia rickettsii. of the 693 nymphal and a ... | 1995 | 8567911 |
| canine ehrlichiosis in egypt: sero-epidemiological survey. | a total of 374 dogs, 252 from five military kennels and 122 privately owned, were tested for ehrlichia canis antibody. sera were tested at a 1:20 dilution by indirect fluorescent antibody with the use of e. canis cell-culture antigen slides. the overall prevalence of e. canis antibody was 33%. antibody prevalence among military dogs (29%) was significantly lower than among privately owned dogs (41%; p < 0.05). the e. canis seroprevalence among dogs infested with ticks (rhipicephalus sanguineus) ... | 1995 | 8539033 |
| demonstration of serum antiplatelet antibodies in experimental acute canine ehrlichiosis. | this report presents evidence for the presence of antiplatelet antibodies in sera of dogs experimentally infected with ehrlichia canis, during the acute phase of the disease. six healthy adult male beagle dogs were inoculated intravenously with blood from a longstanding infected dog with the israel strain 611 of e. canis. thrombocytopenia and concurrent increase in mean platelet volume were the most consistent haematological signs of the disease. the dogs developed an antibody titre to e. canis ... | 1995 | 8533312 |
| characterization of ehrlichial organisms isolated from a wild mouse. | an infectious agent was isolated from the enlarged spleen of a wild mouse, eothenomys kageus, by intraperitoneal inoculation of the spleen homogenate into laboratory mice. the laboratory mice developed splenomegaly, and the agent was maintained by serial passage of spleen homogenates in laboratory mice. the agent in the spleen homogenate was inactivated after incubation at 37 or 50 degrees c. tetracyclines were effective in preventing infection of mice with this agent, but penicillin and sulfona ... | 1993 | 8380184 |
| cytopathic effect, plaque formation, and lysis of ehrlichia chaffeensis grown on continuous cell lines. | ehrlichiae are strict intracellular bacterial pathogens that parasitize leukocytes or other blood cells. only six agents of the tribe ehrlichieae, namely, cowdria ruminantium, neorickettsia helminthoeca, ehrlichia risticii, ehrlichia sennetsu, ehrlichia canis, and ehrlichia chaffeensis, have been adapted to growth in continuous cell lines. e. chaffeensis, the agent of human ehrlichiosis, has been cultured only in a cell line of canine origin. we adapted purified cell-free e. chaffeensis for grow ... | 1994 | 8300201 |
| serologic evidence of canine and equine ehrlichiosis in northeastern united states. | in a retrospective study, indirect fluorescent-antibody staining methods were used to detect immunoglobulins to ehrlichia canis and ehrlichia risticii in canine and equine sera that had originally been analyzed for antibodies to borrelia burgdorferi. analyses of 60 dog serum specimens collected in connecticut and new york state during 1986 revealed antibodies to e. canis in 7 (11.7%) specimens; titration endpoints ranged from 1:40 to 1:320. three of these dogs had anemia. of the 187 equine serum ... | 1993 | 8263167 |
| infections with babesia canis and ehrlichia canis in dogs in zimbabwe. | of 105 dogs examined at a veterinary hospital in harare, zimbabwe, 52 per cent had antibodies reactive with ehrlichia canis in indirect fluorescent antibody tests, 26 per cent had babesia canis parasites in peripheral blood smears and 17 per cent had both infections. none of the dogs with serological evidence of ehrlichiosis had typical e canis morulae detectable in blood smears. the infections were regarded as incidental findings not readily related to the reasons for examination in 46 per cent ... | 1993 | 8236678 |
| serological evidence for antigenic relationships between ehrlichia canis and cowdria ruminantium. | the sera from dogs experimentally infected with ehrlichia canis reacted at very similar titres in indirect fluorescent antibody (ifa) tests with e canis and cowdria ruminantium antigen. in western blots these sera reacted strongly with the immunodominant antigens of e canis (27 kda) and c ruminantium (25 and 32 kda). the dogs experimentally infected with c ruminantium showed no clinical evidence of infection, though the presence of organisms in the blood was demonstrable, for up to two weeks aft ... | 1994 | 8191006 |
| western blot and indirect fluorescent antibody testing for antibodies reactive with ehrlichia canis in sera from apparently healthy dogs in zimbabwe. | sera were collected from apparently healthy dogs in 3 major centres in zimbabwe to establish the prevalence of antibodies reactive with ehrlichia canis by indirect fluorescent antibody (ifa) testing at titres of 1/20 or greater. the overall seroprevalence was 42% (39/93) with dogs from mutare (40%) and harare (33%) having similar rates which were lower than those for bulawayo (68%). the majority of the ifa positive dogs from harare (67%) had no haematological or biochemical evidence of canine eh ... | 1993 | 8176682 |
| human ehrlichiosis in the united states, 1985 to 1990. | to describe the epidemiology, clinical features, laboratory manifestations, response to therapy, and factors related to morbidity and mortality in a large group of patients with ehrlichiosis. | 1994 | 8147546 |
| cultural, molecular, and immunological characterization of the etiologic agent for atypical canine ehrlichiosis. | more than 100 cases of canine ehrlichiosis, with three fatalities, were serologically negative by the indirect immunofluorescent antibody (ifa) test with ehrlichia canis or e. sennetsu antigen but were reactive at titers of 10 to 640 with e. risticii. ehrlichia-like agents were isolated from three such cases. the agents isolated from those cases were morphologically indistinguishable from each other and from a prototype, e. risticii, the etiologic agent of equine monocytic ehrlichiosis, in terms ... | 1994 | 8126175 |
| a rickettsia-like organism from ixodes uriae ticks collected on the kerguelen islands (french subantarctic territories). | a rickettsia-like microorganism was isolated in suckling mice from ixodes uriae ticks collected from penguins breeding on mayes island, kerguelen archipelago, french subantarctic territories. at isolation, this agent mimicked a tick-borne arbovirus. finally, electron microscopy studies of infected suckling mouse livers showed the presence of inclusions filled with pleomorphic microorganism in the cytoplasm of some hepatocytes, sometimes dividing by binary fission and thus of obviously non-viral ... | 1993 | 8105647 |
| reactivity of sera collected from dogs in mutare, zimbabwe, to antigens of ehrlichia canis and cowdria ruminantium. | six of 20 sera from apparently healthy dogs in mutare, zimbabwe, contained antibodies which were reactive with cowdria ruminantium and ehrlichia canis in indirect fluorescent antibody tests at similar titres. in western blots these sera recognised the immunodominant antigen bands of the two organisms. sera with high titres (> 1/80) recognised additional antigen bands of each organism. it was not possible to determine whether these dogs had been exposed to c ruminantium, e canis or other ehrlichi ... | 1994 | 8073593 |
| c-reactive protein and alpha 1-acid glycoprotein levels in dogs infected with ehrlichia canis. | to elucidate whether acute-phase protein responses occur in dogs infected with ehrlichia canis, c-reactive protein (crp) and alpha 1-acid glycoprotein (aag) levels were serially measured in the plasma of five dogs experimentally inoculated with e. canis and 10 sham-inoculated or noninoculated control dogs. the crp concentration was measured by a canine-specific capture enzyme-linked immunosorbent assay, and the aag concentration was measured by a canine-specific radial immunodiffusion method. in ... | 1994 | 8027343 |
| absence of antinuclear antibodies in dogs infected with ehrlichia canis. | 1994 | 8009803 | |
| comparison of pcr with other tests for early diagnosis of canine ehrlichiosis. | the purpose of the study was to compare the sensitivity of pcr with those of cell culture reisolation of ehrlichia canis, the indirect fluorescent antibody test (ifa), and western immunoblotting (wi) in the early diagnosis of canine ehrlichiosis. five german shepherd dogs were intravenously inoculated with 10(7) e. canis-infected dh82 cells. blood was collected on alternate days during a 2-week postinoculation period. mononuclear cell fractions were harvested and used for e. canis reisolation an ... | 1994 | 7929754 |
| reisolation of ehrlichia canis from blood and tissues of dogs after doxycycline treatment. | we present evidence that supports the carrier status of dogs experimentally infected with ehrlichia canis after treatment with doxycycline. canine ehrlichiosis was induced in five dogs by intravenous inoculation with e. canis-infected dh82 cells. all animals developed mild clinical signs of transient fever, body weight loss, thrombocytopenia, and increased gamma globulin levels in plasma. an indirect fluorescent-antibody test (ifa) revealed that all dogs had seroconverted (titer, 5,120) by day 1 ... | 1994 | 7929751 |
| osmotic fragility of erythrocytes in clinically normal dogs and dogs infected with parasites. | the osmotic fragility of the erythrocytes was measured in blood samples collected from randomly selected healthy and infected dogs at a dogs' rescue shelter. the dogs were classified into six groups on the basis of the final diagnoses from clinical, post mortem and laboratory findings. the minimum (less than 5 per cent) and maximum (more than 90 per cent) haemolysis of the erythrocytes of the clinically normal dogs (group 1), occurred in 0.60 per cent and 0.30 per cent solutions of sodium chlori ... | 1994 | 7871255 |