Publications

Pasteurella multocida involved in respiratory disease of wild chimpanzees.	Pasteurella multocida can cause a variety of diseases in various species of mammals and birds throughout the world but nothing is known about its importance for wild great apes. In this study we isolated P. multocida from wild living, habituated chimpanzees from Taï National Park, Côte d'Ivoire. Isolates originated from two chimpanzees that died during a respiratory disease outbreak in 2004 as well as from one individual that developed chronic air-sacculitis following this outbreak. Four isolate ...	2011	21931664
pasteurella multocida zoonotic ascending infection: an unusual cause of tubo-ovarian abscess.	abstract we report a tubo-ovarian abscess due to pasteurella multocida. this zoonotic infection was likely of ascending origin, as pasteurella was also isolated from vaginal swabs.	2011	21923258
efficacy and safety of a new 450 mg/ml florfenicol formulation administered intramuscularly in the treatment of bacterial bovine respiratory disease.	the objective of the study was the safety and efficacy evaluation of a new 450 mg/ml florfenicol formulation in the treatment of naturally occurring respiratory disease when administered intramuscularly, compared with a positive control group treated with the well-established 300 mg/ml formulation. a total of 174 calves, selected from five sites in france and spain, aged from 1 to 17 months, showing severe signs of respiratory disease, were randomly assigned to treatment with either the 300 mg/m ...	2011	21921060
genome sequence of pasteurella multocida subsp. gallicida anand1_poultry.	we report the finished and annotated genome sequence of pasteurella multocida gallicida strain anand1_poultry, which was isolated from the liver of a diseased adult female chicken. the strain causes a disease called "fowl cholera," which is a contagious disease in birds. we compared it with the published genome sequence of pasteurella multocida pm70.	2011	21914901
polymerase chain reaction-restriction fragment length polymorphism analysis of the genes involved in the biosynthesis of the lipopolysaccharide of pasteurella multocida.	the lipopolysaccharide, also known as the somatic antigen or o-antigen, is an important virulence factor of pasteurella multocida. in the current study, the genes involved in the biosynthesis of the outer core region of the lipopolysaccharide, which were obtained from somatic type reference strains and field strains of p. multocida, were subjected to polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) analysis. the pcr-rflp analysis classified 11 out of the 16 serotypes ...	2011	21908287
polymerase chain reaction-based identification of clinically relevant pasteurellaceae isolated from cats and dogs in poland.	a set of polymerase chain reaction (pcr) assays for identification of the most important pasteurellaceae species encountered in cats and dogs were developed. primers for pasteurella multocida were designed to detect a fragment of the kmt, a gene encoding the outer-membrane protein. primers specific to pasteurella canis, pasteurella dagmatis, and pasteurella stomatis were based on the manganese-dependent superoxide dismutase gene (soda) and those specific to [haemophilus] haemoglobinophilus on sp ...	2011	21908285
associations between biovar and virulence factor genes in pasteurella multocida isolates from pigs in spain.	two hundred and five isolates of pasteurella multocida from pigs were phenotypically and genetically characterised by determining their biovar, capsular type, virulence-associated genes and pulsed-field gel electrophoresis (pfge) profiles. all isolates were identified as p multocida subspecies multocida and most were assigned to biovar 3 (58 per cent) and biovar 2 (39.5 per cent). biovar 1 represented 2.4 per cent of the isolates. according to the capsular type, the great majority of the isolate ...	2011	21868435
one-pot three-enzyme synthesis of udp-glcnac derivatives.	a pasteurella multocida n-acetylglucosamine 1-phosphate uridylyltransferase (pmglmu) was cloned and used efficiently with an n-acetylhexosamine 1-kinase (nahk_atcc55813) and an inorganic pyrophosphatase (pmppa) for one-pot three-enzyme synthesis of udp-glcnac derivatives with or without further chemical diversification.	2011	21863157
Decreasing the sialidase activity of multifunctional Pasteurella multocida a2-3-sialyltransferase 1 (PmST1) by site-directed mutagenesis.	Pasteurella multocida a2-3-sialyltransferase 1 (PmST1) is a multifunctional enzyme which has a2-6-sialyltransferase, a2-3-sialidase, and a2-3-trans-sialidase activities in addition to its major a2-3-sialyltransferase activity. The presence of the a2-3-sialidase activity of PmST1 complicates its application in enzymatic synthesis of a2-3-linked sialosides as the product formed can be hydrolyzed by the enzyme. Herein we show that the a2-3-sialidase activity of PmST1 can be significantly decreased ...	2011	21858283
prevalence of respiratory pathogens in diseased, non-vaccinated, routinely medicated veal calves.	the prevalence of respiratory pathogens in diseased veal calves was determined in 24 respiratory disease outbreaks in 15 herds in belgium. bacteria were cultured from nasopharyngeal swabs and seroconversion against viruses and mycoplasma bovis was determined on paired sera. at the individual calf level, mycoplasma species, mannheimia haemolytica and pasteurella multocida, were isolated from 70.5 per cent, 21.5 per cent and 26.0 per cent of swabs, respectively. at the herd level, the presence of ...	2011	21831999
comparative analysis of histophilus somni ibpa with other fic enzymes reveals differences in substrate and nucleotide specificities.	a new family of adenylyltransferases, defined by the presence of a fic domain, was recently discovered to catalyze the addition of adenosine mono-phosphate (amp) to rho gtpases (yarbrough et al., 2009, science, 323:269; worby et al., 2009, mol. cell, 34:93). this adenylylation event inactivates rho gtpases by preventing them from binding to their downstream effectors. we reported that the fic domain(s) of the protein ibpa from the pathogenic bacterium histophilus somni adenylylates mammalian rho ...	2011	21795713
mucoadhesive and ph-sensitive thiolated eudragit microspheres for oral delivery of pasteurella multocida antigens containing dermonecrotoxin.	in this study, cysteine was conjugated to the eudragit to have mucoadhesive and ph-sensitive properties. pasteurella multocida dermonecrotoxin (pmt) is a major virulence factor as a causative agent of atrophic rhinitis (ar) in swine and, therefore, inactivated p. multocida was used as a candidate vaccine in the current study. pmt-loaded thiolated eudragit microspheres (tems) prepared using w/o/w emulsion-solvent evaporation method were characterized to assess their efficacy in oral vaccination. ...	2011	21780423
inhibition of capsular protein synthesis of pasteurella multocida strain p-1059.	a mutant strain pba322 was constructed by electroporation of phagemid containing coding region of antisense rna of the omph gene, encoding 39 kda capsular protein or omph, into parental strain p-1059 (serovar a:3) of pasteurella multocida and determined the pathogenicity in mice and chickens. grayish colonies of the mutant, indicating loss of capsule synthesis, were observed by the obliquely-transmitted light stereomicroscope when compared to iridescent colonies of the parental strain. moreover, ...	2011	21747216
expression of matrix metalloproteinase-9 and -12 in porcine lung infections.	matrix metalloproteinases (mmps) play a variety of roles during organogenesis, in the immune response and during acute and chronic diseases as well as in tissue remodelling. during the last decade, the pig has become used increasingly as a model for human diseases; however, studies on the expression of porcine mmps are limited. in the present study species-specific antibodies were produced to investigate the expression of mmp-9 and mmp-12 immunohistochemically in lungs from pigs infected with ac ...	2011	21737093
microbiological identification and analysis of swine tonsils collected from carcasses at slaughter.	the primary objective of this 7-month study was to determine the prevalence of porcine pathogens of the tonsil of the soft palate of swine at slaughter. additional objectives were to determine if sampling the carcasses of normal or abnormal hogs provided different microbiological profiles and if the slaughter plant provides a feasible sampling frame and environment for detecting and monitoring important pathogens in tonsils that have health implications for both swine and humans. a total of 395 ...	2011	21731180
relationship of in vitro minimum inhibitory concentrations of tilmicosin against mannheimia haemolytica and pasteurella multocida and in vivo tilmicosin treatment outcome among calves with signs of bovine respiratory disease.	objective-to determine associations between in vitro minimum inhibitory concentrations (mics) of tilmicosin against mannheimia haemolytica and pasteurella multocida and in vivo tilmicosin treatment outcome among calves with clinical signs of bovine respiratory disease (brd). design-observational, retrospective, cohort study. animals-976 feeder calves with clinical signs of brd enrolled in 16 randomized clinical trials. procedures-records of clinical trials from october 26, 1996, to november 15, ...	2011	21718206
deep sternal wound infection due to pasteurella multocida: the first case report and review of literature.	pasteurella multocida is a gram-negative bacterium recovered from a wide variety of wild and domestic animals and has mostly been associated with infection following animal bites. we present the first reported case of a patient who developed a postoperative sternal wound infection due to p. multocida complicated by bloodstream infection. the outcome was favorable following surgical debridement and antimicrobial therapy. we also review the literature regarding p. multocida postoperative wound inf ...	2011	21713430
investigations into an outbreak of corvid respiratory disease associated with pasteurella multocida.	the possible cause of disease and mortality in corvids on an outdoor pig unit in the north of england between august 2007 and march 2008 was investigated. nine carrion crows (corvus corone corone) and nine rooks (corvus frugilegus), comprising five live-caught birds with clinical signs of respiratory disease, one live-caught bird without respiratory disease, and 12 birds submitted dead were examined. clinical signs, gross and histopathological examination, microbiology and toxicology indicated t ...	2011	21711193
combinations of macrolide resistance determinants in field isolates of mannheimia haemolytica and pasteurella multocida.	respiratory tract infections in cattle are commonly associated with the bacterial pathogens mannheimia haemolytica and pasteurella multocida. these infections can generally be successfully treated in the field with one of several groups of antibiotics, including macrolides. a few recent isolates of these species exhibit resistance to veterinary macrolides with phenotypes that fall into three distinct classes. the first class has type i macrolide, lincosamide, and streptogramin b antibiotic resis ...	2011	21709086
investigating the candidacy of lipopolysaccharide-based glycoconjugates as vaccines to combat mannheimia haemolytica.	inner core lipopolysaccharide (lps) has been shown to be conserved in the majority of veterinary strains from the species mannheimia haemolytica, actinobacillus pleuropneumoniae and pasteurella multocida and as such is being considered as a possible vaccine antigen. the proof-in-principle that a lps-based antigen could be considered as a vaccine candidate has been demonstrated from studies with monoclonal antibodies raised to the inner core lps of mannheimia haemolytica, which were shown to be b ...	2011	21701793
pasteurella multocida septicaemia in a patient on haemodialysis.	this case report details the presentation of a 76-year-old man on haemodialysis who rapidly developed overwhelming sepsis over a 24 h period with isolation of the pathogen pasteurella multocida in blood cultures. despite treatment with antibiotics shown to have good activity against this pathogen, the patient died. during further questioning, it transpired that the patient had allowed his dog to lick him, including the neck haemodialysis cannula, though no direct trauma in the form of bites or s ...	2009	21687004
kinetics of the response of four positive acute phase proteins in pigs experimentally infected with toxigenic pasteurella multocida.	in the present study, acute phase proteins (app) responses in pigs after infection with toxigenic strain of pasteurella multocida (pm) were evaluated. twelve piglets from a herd free from toxigenic pm were used. six of them were infected intranasally with pm. crp, hp, saa and pig-map concentrations were measured using commercial elisa tests. crp and hp were significantly induced from 2 days post inoculation (dpi). the concentration of hp in inoculated pigs remained elevated until the end of the ...	2011	21676559
a review of hemorrhagic septicemia in cattle and buffalo.	hemorrhagic septicemia (hs), an acute, fatal and septicemic disease of cattle and buffaloes caused by pasteurella multocida, is important in tropical regions of the world, especially in african and asian countries. the prevalence of disease has been well documented with predominant isolation of p. multocida serotypes b:2 and e:2. conventional methods of identification such as serotyping, biotyping, antibiogram determination and pathogenicity as well as molecular methods (p. multocida-specific po ...	2011	21676341
pasteurella multocida lipopolysaccharide: the long and the short of it.	pasteurella multocida is a capsulated, gram-negative cocco-bacillus that can cause serious disease in a wide range of mammals and birds. p. multocida strains are classified into 16 serovars based on lipopolysaccharide (lps) antigens. lps is an essential virulence factor of p. multocida; mutants expressing severely truncated lps are completely attenuated in chickens. lps is also a major immunogen of p. multocida and protection against infections caused by p. multocida is generally considered to b ...	2011	21664074
mucosal immunization provides better protection than subcutaneous immunization against pasteurella multocida (b:2) in mice preimmunized with the outer membrane proteins.	to investigate the effect of boosting immunity via mucosal route vis-a-vis parenteral route in the mouse model of haemorrhagic septicaemia, mice preimmunized with omp of pasteurella multocida (b:2) were immunized with 10(2) cfu of p. multocida via intranasal and subcutaneous routes. mice were challenged through intranasal route (natural route of infection) with 10(8) cfu 14 days after immunization. group of mice which were immunized intranasally showed significant protection (p < 0.05) of 88% as ...	2011	21633791
enzymatic actions of pasteurella multocida toxin detected by monoclonal antibodies recognizing the deamidated alpha subunit of the heterotrimeric gtpase g(q).	pasteurella multocida toxin (pmt) is a virulence factor responsible for the pathogenesis of some pasteurellosis. pmt exerts its toxic effects through the activation of heterotrimeric gtpase (g(q) , g(12/13) , and g(i) )-dependent pathways, by deamidating a glutamine residue in the α subunit of these gtpases. however, the enzymatic characteristics of pmt are yet to be analyzed in detail, because the deamidation has only been observed in cell-based assays. in this study, we developed rat monoclona ...	2011	21624053
multilocus sequence typing of a global collection of pasteurella multocida isolates from cattle and other host species demonstrates niche association.	abstract: background: pasteurella multocida causes disease in many host species throughout the world. in bovids, it contributes to bovine respiratory disease (brd) and causes haemorrhagic septicaemia (hs). previous studies have suggested that brd-associated p. multocida isolates are of limited diversity. a multilocus sequence typing (mlst) scheme for p. multocida was used to determine whether the low levels of diversity reported are due to the limited discriminatory power of the typing method us ...	2011	21612618
comamonas testosteronan synthase, a bifunctional glycosyltransferase that produces a unique heparosan polysaccharide analog.	glycosaminoglycans [gags] are linear hexosamine-containing polysaccharides. these polysaccharides are synthesized by some pathogenic bacteria to form an extracellular coating or capsule. this strategy forms the basis of molecular camouflage since vertebrates possess naturally-occurring gags that are essential for life. a recent sequence database search identified a putative protein from the opportunistic pathogen comamonas testosteroni that exhibits similarity to the pasteurella multocida glycos ...	2011	21610195
capsular serotyping of pasteurella multocida from various animal hosts - a comparison of phenotypic and genotypic methods.	one hundred and fourteen strains of pasteurella multocida were isolated from different domestic animals species (cattle, buffalo, sheep, goat, pig, rabbit, dog, cat), avian species (chicken, duck, turkey) and wild animals (deer, tiger, orang utan, marmoset). the serogroups of p. multocida were determined by both conventional capsular serotyping and a multiplex pcr assay targeting specific capsular genes. based on the conventional serotyping method, the 114 strains of p. multocida were subtyped i ...	2011	21602769
structural analyses of the core oligosaccharide from the lipopolysaccharide of bovine and ovine strains of mannheimia haemolytica serotype 2.	previous structural studies in our laboratory on lipopolysaccharide derived core oligosaccharide had identified a conserved inner core structure in several strains of the veterinary pathogens mannheimia haemolytica, actinobacillus pleuropneumoniae and pasteurella multocida. in this study we describe the elucidation of the core oligosaccharide structure of two strains from m. haemolytica serotype 2. structural information was established by a combination of monosaccharide and methylation analyses ...	2011	21601179
chemoenzymatic synthesis of c8-modified sialic acids and related α2-3- and α2-6-linked sialosides.	naturally occurring 8-o-methylated sialic acids, including 8-o-methyl-n-acetylneuraminic acid and 8-o-methyl-n-glycolylneuraminic acid, along with 8-o-methyl-2-keto-3-deoxy-d-glycero-d-galacto-nonulosonic acid (kdn8me) and 8-deoxy-kdn were synthesized from corresponding 5-o-modified six-carbon monosaccharides and pyruvate using a sialic acid aldolase cloned from pasteurella multocida strain p-1059 (pmnana). in addition, α2-3- and α2-6-linked sialyltrisaccharides containing neu5ac8me and kdn8deox ...	2011	21592790
cellular and molecular action of the mitogenic protein-deamidating toxin from pasteurella multocida.	the mitogenic toxin from pasteurella multocida (pmt) is a member of the dermonecrotic toxin family, which includes toxins from bordetella, escherichia coli and yersinia. members of the dermonecrotic toxin family modulate g-protein targets in host cells through selective deamidation and/or transglutamination of a critical active site gln residue in the g-protein target, which results in the activation of intrinsic gtpase activity. structural and biochemical data point to the uniqueness of pmt amo ...	2011	21569202
association of porcine circovirus type 2 with vascular lesions in porcine pneumonia.	the aim of this study was to evaluate the vasculature in porcine circovirus type 2-infected (pcv2-infected) lungs and to identify the pcv2 subtypes involved in porcine pneumonia. pulmonary samples from 140 pigs, 2 weeks to 7 months of age, from 36 hungarian commercial herds with clinical signs of respiratory disease were examined for the presence of respiratory pathogens, with bacterial culture, pathologic evaluation, and immunohistochemistry for pcv2, porcine reproductive respiratory syndrome v ...	2011	21551427
pmst2 - a novel pasteurella multocida glycolipid {alpha}2-3-sialyltransferase.	pasteurella multocida is a multi-species pathogen that causes diseases in animals and humans. sialyltransferase activity has been detected in multiple pasteurella multocida strains and sialylation has been shown to be important for the pathogenesis of pasteurella multocida. three putative sialyltransferase genes have been identified in pasteurella multocida genomic strain pm70. we have reported previously that a pm0188 gene homolog in p. multocida strain p-1059 (atcc 15742) encodes a multifuncti ...	2011	21515586
molecular epidemiology of pasteurella multocida in dairy and beef calves.	the molecular epidemiology of pasteurella multocida has rarely been studied at the farm level in cattle. the aim of this study was to determine whether single or multiple strains of p. multocida tend to exist within farms. molecular characterisation was carried out on isolates obtained from nasal swabs from 105 calves from 32 randomly selected beef and dairy farms located throughout scotland, and from 131 calves from 20 farms in the mayenne region of france, where sampling occurred in response t ...	2011	21511409
enzymatic placement of 6-o-sulfo groups in heparan sulfate.	heparan sulfate is a highly sulfated polysaccharide that exhibits important physiological and pathological functions. the glucosamine residue of heparan sulfate can carry sulfo groups at the 2-n, 3-o, and 6-o positions, leading to diverse polysaccharide structures. 6-o-sulfation at the glucosamine residue contributes to a wide range of biological functions. here, we report a method for controlling the positioning of 6-o-sulfo groups in oligosaccharides. this was achieved by synthesizing oligosac ...	2011	21506605
demonstration of the metaphylactic use of gamithromycin against bacterial pathogens associated with bovine respiratory disease in a multicentre farm trial.	on five commercial cattle rearing sites across europe, a total of 802 young cattle at high risk of developing bovine respiratory disease (brd) associated with the bacterial pathogens mannheimia haemolytica or pasteurella multocida and/or mycoplasma bovis were enrolled into a multicentre, controlled field trial. half were treated with a single dose of gamithromycin at 6 mg/kg bodyweight by subcutaneous injection and half received an injection of a saline placebo as the control. all animals were o ...	2011	21493573
influence of a pig respiratory disease on the pharmacokinetic behaviour of amoxicillin after oral ad libitum administration in medicated feed.	the pharmacokinetic properties of amoxicillin in healthy and respiratory-diseased pigs were studied, after ad libitum administration of medicated feed. in addition, amoxicillin dose linearity and drug penetration into respiratory tract tissues were evaluated in diseased animals. the respiratory disease involves porcine reproductive and respiratory syndrome virus and bacterial agents such as pasteurella multocida, bordetella bronchiseptica and streptococcus suis. typical clinical signs and gross ...	2011	21492192
structural modification of sanguinarine and chelerythrine and their antibacterial activity.	in this study, five derivatives of sanguinarine (1) and chelerythrine (2) were prepared, with 1 and 2 as starting materials, by reduction, oxidation and nucleophilic addition to the iminium bond c=n(+). the structures of all compounds were elucidated on account of their ms, (1)h-nmr and (13)c-nmr data. the antibacterial activities of all compounds were screened, using staphylococcus aureus, escherichia coli, aeromonas hydrophila and pasteurella multocida as test bacteria. the minimum bacteriosta ...	2011	21491327
the forgotten giants behind louis pasteur: contributions by the veterinarians toussaint and galtier.		2010	21466009
plasma concentrations resulting from florfenicol preparations given to pigs in their drinking water.	florfenicol administered through the drinking water has been recommended as a metaphylactic antibacterial drug to control outbreaks of respiratory diseases in pigs caused by strains of actinobacillus pleuropneumoniae and pasteurella multocida, yet it is difficult to pinpoint in practice when the drug is given metaphylactically or therapeutically. further, pigs are likely to reject florfenicol-medicated water, and plasma concentrations of the drug are likely to be marginal for diseases caused by ...	2011	21454858
[fatal clarkson syndrome mimicking a septic shock].		2011	21440390
genetic diversity and associated pathology of pasteurella multocida isolated from porcine pneumonia.	pasteurella multocida is a widespread respiratory pathogen in pigs associated with atrophic rhinitis and contributing to aggravation of the pulmonary lesions. the aims of the present study were to characterize isolates of p. multocida from porcine bronchopneumonia by pulsed-field gel electrophoresis (pfge), pcr based capsular typing and multilocus sequence typing (mlst) and to compare clonal complexes outlined with the type of histological lung lesions to investigate if a correlation between clo ...	2011	21439738
haemolytic and cytotoxic activities of the tween 80-extracted putative haemolysin of pasteurella multocida b:2.	the objective of this study was to investigate the haemolytic and cytotoxic activity of pasteurella multocida b:2 strains, originally from cases of haemorrhagic septicaemia in cattle. all six p. multocida b:2 strains were non-haemolytic on sheep blood agar (sba) and horse blood agar (hba) when grown aerobically and on sba anaerobically but they were haemolytic on hba when grown anaerobically. no haemolytic activity against horse red blood cells was detected in culture supernates from aerobically ...	2011	21420800
medical microbiology	the genus pasteurella was originally proposed and described by trevisan in 1887. it consisted of a group of nonmotile, small (0. 7 μm by 0. 5 μm), gram-negative coccobacilli often exhibiting a characteristic type of bipolar staining (fig. 29-1). most members of this genus are associated with severe, life-threatening systemic diseases involving both hemorrhagic pneumonia and septicemia. the first pathogen to be ...	1996	21413268
molecular basis of macrolide, triamilide, and lincosamide resistance in pasteurella multocida from bovine respiratory disease.	the mechanism of macrolide-triamilide resistance in pasteurella multocida has been unknown. during whole-genome sequencing of a multiresistant bovine p. multocida isolate, three new resistance genes, the rrna methylase gene erm(42), the macrolide transporter gene msr(e), and the macrolide phosphotransferase gene mph(e), were detected. the three genes were pcr amplified, cloned into suitable plasmid vectors, and shown to confer either macrolide-lincosamide resistance [erm(42)] or macrolide-triami ...	2011	21402855
cloning, expression and protective capacity of 37 kda outer membrane protein gene (omph) of pasteurella multocida serotype b:2.	the major outer membrane protein (omph) of 4 local malaysian strains of pasteurella multocida serotype b:2 were characterized in comparison to atcc strains. three major peptide bands of mw 26, 32 and 37 kda were characterized using sdspage. two of these fragments, the 32 kda and 37 kda were observed to be more reactive with a mouse polyclonal antiserum in all of the local isolates as well as the atcc strains in a western blot. however, the 32 kda fragment was found to cross react with other gram ...	2010	21399583
penetration of oxytetracycline into the nasal secretions and relationship between nasal secretions and plasma oxytetracycline concentrations after oral and intramuscular administration in healthy pigs.	the penetration of oxytetracycline (otc) in plasma and nasal secretions of healthy pigs was evaluated during the first study, in response to oral dose of 20ôçâmg of otc per kg of body weight (bwt) per day as a 400ôçâmg/kg feed medication (nôçâ=ôçâ5) and to intramuscular (i.m.)-administered formulations at 10ôçâmg/kg bwt (nôçâ=ôçâ5), 20ôçâmg/kg bwt (nôçâ=ôçâ5), 40ôçâmg/kg bwt (nôçâ=ôçâ5). concentrations of otc in plasma and nasal secretions were determined by a validated ultra-high performance li ...	2011	21395609
pathology, tissue metalloproteinase transcription and haptoglobin responses in mice after experimental challenge with different isolates of pasteurella multocida obtained from cases of porcine pneumonia.	pasteurella multocida is a major cause of porcine pneumonia, but the pathogenesis of the disease is poorly defined. the aim of this study was to further understand the host response to infection by use of a mouse model of p. multocida pneumonia. twenty female mice were divided into four groups (n=5). three groups were infected with one of three isolates of p. multocida isolated from clinical cases of chronic porcine pneumonia with necrotizing, suppurative and non-suppurative lesions, respectivel ...	2011	21388634
pasteurella multocida heddleston serovars 1 and 14 express different lipopolysaccharide structures but share the same lipopolysaccharide biosynthesis outer core locus.	pasteurella multocida strains are classified using the heddleston lipopolysaccharide (lps) serotyping scheme into 16 serovars. understanding the structural and genetic basis for this lps typing scheme is important because protection against infections caused by p. multocida is generally considered to be serovar specific. here we show that the serovar 14 type strain p2225 and the serovar 1 strains x73 and vp161 express similar lps structures. however, the serovar 14 lps lacks the terminal phospho ...	2011	21371830
crystal structure and immunogenicity of the class c acid phosphatase from pasteurella multocida.	pasteurella multocida is a pathogen of veterinary and medical importance. here, we report the 1.85å resolution crystal structure of the class c acid phosphatase from this organism (denoted rpmccap). the structure shows that rpmccap exhibits the same haloacid dehalogenase fold and dimeric assembly as the class c enzyme from haemophilus influenzae. formation of the dimer in solution is demonstrated using analytical ultracentrifugation. the active site is devoid of a magnesium ion due to the presen ...	2011	21371420
a novel erm monomethyltransferase in antibiotic-resistant isolates of mannheimia haemolytica and pasteurella multocida.	mannheimia haemolytica and pasteurella multocida are aetiological agents commonly associated with respiratory tract infections in cattle. recent isolates of these pathogens have been shown to be resistant to macrolides and other ribosome-targeting antibiotics. direct analysis of the 23s rrnas by mass spectrometry revealed that nucleotide a2058 is monomethylated, consistent with a type i erm phenotype conferring macrolide-lincosamide resistance. the erm resistance determinant was identified by fu ...	2011	21371136
epidemiological characteristics of pulmonary pneumocystosis and concurrent infections in pigs in jeju island, korea.	epidemiological characteristics of swine pulmonary pneumocystis (p.) carinii and concurrent infections were surveyed on jeju island, korea, within a designated period in 172 pigs submitted from 54 farms to the department of veterinary medicine, jeju national university. the submitted cases were evaluated by histopathology, immunohistochemistry, pcr/rt-pcr, and bacteriology. p. carinii infection was confirmed in 39 (22.7%) of the 172 pigs. histopathologically, the lungs had moderate to severe lym ...	2011	21368558
domestic sheep (ovis aries) pasteurellaceae isolates from diagnostic submissions to the caine veterinary teaching center (1990-2004).	a retrospective study of pasteurellaceae isolated from domestic sheep (ovis aries) was conducted. the aim was to identify pasteurellaceae present in animals that were clinically healthy and others with evidence of respiratory disease. the bacteria had been isolated from samples submitted to the university of idaho caine veterinary teaching center as part of disease diagnostic testing. the 844 isolates identified mainly three species of pasteurellaceae: mannheimia haemolytica, pasteurella multoci ...	2011	21345625
outbreaks of pleuritis and peritonitis in calves associated with pasteurella multocida capsular type b strain.	three dairy calf-rearing properties experienced high mortality in calves during 2008 and 2009. affected calves were aged 13-18 weeks (farm i), 6 months (farm ii), and 2-11 weeks (farm iii), and the mortality rate was 22/175 (13%), 5/80 (6%), and 60/900 (7%), respectively. clinical and laboratory findings: affected calves rapidly became moribund, were in respiratory distress, and had a fever (40-41°c). post-mortem examination of nine calves revealed fibrinopurulent pleuritis, pericarditis, and pe ...	2011	21328156
causes of mortality in commercial organic layers in denmark.	a longitudinal study investigated the courses of mortality in commercial free-range organic layer flocks in denmark. in total, 15 organic egg-producing flocks from 11 farms were randomly selected among 80 farms registered in denmark. four farms with confined egg production on deep litter were included for comparison. flock sizes ranged from 2260 to 5940 layers. the flocks were monitored from introduction to the layer farm until slaughter. flock mortalities ranged from approximately 2% to 91%, wi ...	2010	21313846
pasteurella multocida bacteremia, spontaneous bacterial peritonitis and septic arthritis in a cirrhotic patient.		2010	21311647
occurrence and associated lesions of pasteurella multocida in porcine bronchopneumonia.	with the aim to extend the present knowledge on possible systemic spreading of pasteurella multocida in pigs with bronchopneumonia, the occurrence and associated lesions of p. multocida were described by comparing cultural detection, pathological evaluation and in situ hybridization of p. multocida in lungs, hearts and kidneys from cases of porcine bronchopneumonia. p. multocida was cultivated from the lung lesions in 114 out of a total of 148 cases of porcine bronchopneumonia. among the 114 cas ...	2011	21296510
necrotizing fasciitis complicated by fatal multiple organ dysfunction due to pasteurella multocida infection: a case report.	necrotizing fasciitis is rarely caused by pasteurella multocida, a facultative anaerobic gram-negative coccobacillus found in the flora of the oro-gastrointestinal tract of many animals. we describe a rare case with overwhelming septicaemia resulting in multiple organ failure.	2010	21268955
outbreak of polyarthritis in lambs attributed to pasteurella multocida.		2010	21257563
prevalence of pasteurella multocida and other respiratory pathogens in the nasal tract of scottish calves.	the prevalence of pasteurella multocida, a cause of bovine respiratory disease, was studied in a random sample of beef suckler and dairy farms throughout scotland, by means of a cross-sectional survey. a total of 637 calves from 68 farms from six geographical regions of scotland were sampled between february and june 2008. deep nasal swabs were taken, and samples that were culture-positive for p multocida were confirmed by pcr. prevalence of p multocida was 17 per cent (105 of 616 calves); 47 pe ...	2010	21257416
multiplex pcr assay for detection of actinobacillus pleuropneumoniae, pasteurella multocida and haemophilus parasuis in lungs of pigs from a slaughterhouse.	multiplex pcr has been developed for parallel identification of actinobacillus pleuropneumoniae, pasteurella multocida and haemophilus parasuis, important pathogens of swine, responsible for considerable economic losses in swine industry. multiplex pcr and bacteriological cultivation was used to analyze lung samples from slaughterhouse pigs. from a total of 219 lung samples, 164 (74.9 %) were positive for p. multocida, 45 (20.5 %) for a. pleuropneumoniae and 4 (1.83 %) for h. parasuis. bacteriol ...	2010	21253912
immunogenicity of iron-regulated outer membrane proteins of pasteurella multocida b:2 in mice model.	the present study was conducted to investigate the role of iron-regulated outer membrane proteins (iromp) of pasteurella multocida b:2 in mice as potential immunogens. outer membrane proteins extracted from p. multocida b:2 grown under normal (omp) and iron-deficient (iromp) conditions were subjected to discontinuous sds-page. nine polypeptides of mw ranging from 85.1 to 16.7 kda from omp preparations and two additional polypeptides of mw 95.4 and 89.1 kda from iromp preparations were observed w ...	2010	21250599
transmission of swine pathogens: different means, different needs.	there seems to be two main types of pathogens that cause diseases in swine: those that are mainly introduced through direct pig contacts, and those that are often, and in some situations mainly introduced by indirect transmission means. in this review, the mange mite (sarcoptes scabiei), toxigenic pasteurella multocida and brachyspira hyodysenteriae will be used as examples of the first type, and foot and mouth disease virus, mycoplasma hyopneumoniae and porcine reproductive and respiratory synd ...	2011	21241537
plasma and tissue pharmacokinetics of danofloxacin in healthy and in experimentally infected chickens with pasteurella multocida.		2010	21219355
influence of methylation on the antibacterial properties of triclosan in pasteurella multocida and pseudomonas aeruginosa variant strains.	the opportunistic bacterium pasteurella multocida is extremely susceptible to the hydrophobic biocide triclosan by virtue of its markedly permeable outer membrane, while the nosocomial pathogen pseudomonas aeruginosa is intrinsically resistant to levels far exceeding the triclosan aqueous solubility limit. widespread incorporation of triclosan in health and personal care products has resulted in its concomitant accumulation with metabolites such as methyl triclosan in environmental and biologica ...	2010	21194793
antigenicity of partial fragments of recombinant pasteurella multocida toxin.	pasteurella multocida serogroup d strain, which produces p. multocida toxin (pmt), is a widespread and harmful pathogen of respiratory diseases such as pneumonia and progressive atrophic rhinitis (par) in swine. vaccination has been considered the most desirable and effective approach for controlling the diseases caused by toxigenic p. multocida. to investigate the antigenicity and immunogenicity of partial fragments of recombinant pmt, recombinant proteins of the n-terminal (pmt-a), middle (pmt ...	2010	21193834
pasteurella multocida epiglottitis: a review and report of a new case with associated chronic lymphocytic leukemia.	pasteurella multocida is a gram-negative coccobacillus that primarily affects animals. p multocida infections in humans are usually associated with animal contact. to the best of our knowledge, only 7 cases of p multocida epiglottitis have been previously reported in the english-language literature; none of these cases occurred in a patient with chronic lymphocytic leukemia. we describe what we believe is the first reported case of p multocida epiglottitis in a patient with chronic lymphocytic l ...	2010	21174269
interleukin-6 expression in response to innate immune regulatory factor stimulation.	innate immune regulatory factor (iirf) is a serum-derived biological response modifier, or agent that modifies/bolsters the pathogenic immune response. iirf has been shown to provide protection (e.g., increase host survival) against a variety of pathogenic challenges including salmonella typhimurium and pasteurella multocida. in this report, we analyzed cytokine production in mice, whole blood and cultured cells in response to iirf challenge. when iirf was administered to mice, it stimulated the ...	2010	21131162
pig trotters lung--novel domestic transmission of pasteurella multocida.	pasteurella multocida is a non-motile, faculatively-anaerobic, gram-negative bacillus associated with a spectrum of human disease. direct and indirect zoonotic transmission is recognised with animal bites being most frequently encountered as a result of salivary colonisation in farm and domestic animals. despite the prevalence of p. multocida in swine herds, the relationship between porcine colonisation and human disease is poorly established. this lesson reports a previously unrecognised mode o ...	2010	21117392
immunomodulatory activity of methanolic leaf extract of moringa oleifera in animals.	aim: the aim of the present study was to investigate the immunomodulatory action of methanolic extract of moringa oleifera (memo) in an experimental model of immunity. the cellular immunity was evaluated using neutrophil adhesion test, cyclophosphamide induced neutropenia and carbon clearance assay, whereas, humoral immunity was tested by mice lethality test, serum immunoglobulin estimation and indirect haemagglutination assay in animals. administration of memo (250 and 750 mg/kg, po) and ocimum ...	2010	21090530
analysis of the polymerization initiation and activity of pasteurella multocida heparosan synthase pmhs2, an enzyme with glycosyltransferase and udp-sugar hydrolase activity.	heparosan synthase catalyzes the polymerization of heparosan (-4glcuaβ1-4glcnacα1-)(n) by transferring alternatively the monosaccharide units from udp-glcua and udp-glcnac to an acceptor molecule. details on the heparosan chain initiation by pasteurella multocida heparosan synthase pmhs2 and its influence on the polymerization process have not been reported yet. by site-directed mutagenesis of pmhs2, the single action transferases pmhs2-glcua(+) and pmhs2-glcnac(+) were obtained. when incubated ...	2010	21084307
characterization of outer membrane proteins participating in iron transport in pasteurella multocida serotype a3.	iron-regulated outer membrane proteins (iromps) of p. multocida serotype a3, which function as receptors for complexes containing iron ions, are induced by iron deficiency in the bacterial growth environment. analysis of an electrophoresis image of proteins isolated from bacteria grown on medium supplemented with 2,2'-dipyridyl revealed expression of 16 new proteins that were not noted in the case of the bacteria grown in standard conditions, with molecular weights from 30 to 160 kda. induction ...	2010	21077440
antibiotic susceptibility of bacteria isolated from respiratory tract of pigs in poland between 2004 and 2008.	antibiotic susceptibility of bacteria isolated from nasal swabs and lungs of pigs, to 16 commonly used antibiotics, was determined by disc diffusion test. beta-lactams showed the best activity against streptococcus suis (s. suis) (> 99% of susceptible strains). the lowest sensitivity of s. suis was evidenced to: tylosin, tetracycline and neomycin (50%, 40% and 25%, respectively). isolates of escherichia coli (e. coli) demonstrated the highest susceptibility to cephalosporin (85% strains), gentam ...	2010	21077428
genetic diversity of pasteurella species isolated from european vespertilionid bats.	pasteurella are an important cause of fatal infections in free-ranging bats, but the genetic diversity of bat-derived strains is unclear. in the current study, 81 pasteurella strains associated with pneumonia, severe organ necroses and systemic infection in free-ranging european vespertilionid bats were characterized by biochemical and molecular typing methods. genetic relationships and subspecies status of pasteurella multocida strains were determined by comparative 16s rdna and rpob gene seque ...	2010	21050683
the impact of environmental contamination with antibiotics on levels of resistance in soil bacteria.	this study examined the effects of tetracycline and streptomycin on microorganisms in three different soil habitats: forest soil, agricultural soil, and compost. these antibiotics are commonly used in both medical and veterinary therapy as well as in the production of plant biomass and until quite recently, the production of animal biomass. microcosms were used as model systems in which the number of microorganisms in environments containing different amounts of antibiotics was analyzed. the min ...	2010	21043273
loop-mediated isothermal amplification assay targeting the ompa gene for rapid detection of riemerella anatipestifer.	a novel loop-mediated isothermal amplification (lamp) assay was developed and evaluated for the detection of riemerella anatipestifer (ra) infection. the lamp assay exhibited a higher sensitivity than conventional polymerase chain reaction (pcr) and microbial isolation. the specificity of the assay was determined by restriction enzyme digestion of the lamp products and detection of escherichia coli, salmonella enterica and pasteurella multocida. the lamp assay was able to detect ra effectively i ...	2010	21040782
[pasteurella multocida meningoencephalitis].		2010	21036520
treatment of pasteurella multocida (fowl cholera) infection in wild ducks with autogenous bacterin and penicillin.		1946	21013347
cloning and characterization of a viral α2-3-sialyltransferase (vst3gal-i) for the synthesis of sialyl lewisx.	sialyl lewis(x) (sle(x), siaα2-3galβ1-4(fucα1-3)glcnacβor) is an important sialic acid-containing carbohydrate epitope involved in many biological processes such as inflammation and cancer metastasis. in the biosynthetic process of sle(x), α2-3-sialyltransferase-catalyzed sialylation generally proceeds prior to α1-3-fucosyltransferase-catalyzed fucosylation. for the chemoenzymatic synthesis of sle(x) containing different sialic acid forms, however, it would be more efficient if diverse sialic ac ...	2010	20978012
enzymatic synthesis and properties of glycoconjugates with legionaminic acid as a replacement for neuraminic acid.	in addition to sialic acid, bacteria produce several other nonulosonic acids, including legionaminic acid (leg). this has exactly the same stereochemistry as sialic acid, with the added features of 9-deoxy and 7-amino groups. in order to explore the biological effects of replacing sialic acid residues (neu5ac) in glycoconjugates with leg in its diacetylated form, diacetyllegionaminic acid (leg5ac7ac), we tested cmp-leg5ac7ac as a donor substrate with a selection of bacterial and mammalian sialyl ...	2010	20978010
contribution of rob-1 and pbp3 mutations to the resistance phenotype of a β-lactamase-positive amoxicillin/clavulanic acid-resistant haemophilus influenzae carrying plasmid pb1000 in italy.	plasmid pb1000 bearing bla(rob-1) is responsible for high-level β-lactam resistance in haemophilus influenzae as well as in pasteurella multocida and haemophilus parasuis isolates from spain. here, we explore the presence of rob-1 in italy and investigate the relative contribution of penicillin-binding protein 3 (pbp3) mutations and rob-1 to the β-lactam resistance phenotype in h. influenzae.	2010	20978001
a bighorn sheep die-off in southern colorado involving a pasteurellaceae strain that may have originated from syntopic cattle.	we investigated a pasteurellosis epizootic in free-ranging bighorn sheep (ovis canadensis) wherein a pasteurellaceae strain carried by syntopic cattle (bos taurus) under severe winter conditions appeared to contribute to pneumonia in affected bighorns. twenty-one moribund or dead bighorn sheep were found on the "fossil ridge" herd's winter range, colorado, usa, between 13 december 2007 and 29 february 2008. eight carcasses examined showed gross or microscopic evidence of acute to subacute fibrin ...	2010	20966277
pasteurella multocida toxin-stimulated osteoclast differentiation is b cell dependent.	pasteurella multocida is a gram-negative bacillus that infects a number of wild and domestic animals, causing respiratory diseases. toxigenic pasteurella multocida strains produce a protein toxin (pmt) that leads to atrophic rhinitis in swine due to enhanced osteoclastogenesis and the inhibition of osteoblast function. we show that pmt-induced osteoclastogenesis is promoted by an as-yet-uncharacterized b-cell population. the toxin, however, is not acting at the level of hematopoietic stem cells, ...	2010	20956572
monitoring the health and production of household jinding ducks on hatia island of bangladesh.	duck rearing is an important component of sustainable living in poor rural communities, especially as a source of subsistence. a study was conducted on 118 households (n = 1,373 jinding ducks, anas platyrhynchus) from december 2002 to february 2004 on hatia island in bangladesh with the aim of identifying the factors that limit the health and production of jinding ducks. overall duck mortality was 29.3%, with disease (19.7%) being a more significant factor than predation (9.6%; p = 0.001). duck ...	2010	20936346
bactericidal activity of porcine neutrophil secretions.	antimicrobial proteins in neutrophil granules exert their bactericidal activity both within the neutrophil phagolysosome and as components of neutrophil extracellular traps. this study evaluated the bactericidal activity of porcine neutrophil secretions against four bacterial pathogens of swine. porcine neutrophils were treated with or without phorbol myristate acetate (pma), then the resulting supernatants were incubated with escherichia coli k-12, streptococcus suis, actinobacillus suis, or pa ...	2010	20932586
impact of early versus later fluoroquinolone treatment on the clinical; microbiological and resistance outcomes in a mouse-lung model of pasteurella multocida infection.	the early curative uses of antimicrobial drugs such as fluoroquinolones before the onset of symptoms in veterinary medicine may be regarded as irrational antibiotic consumption. however, it should be stressed that in early curative antimicrobial treatment as in metaphylaxis, the bacterial burden at the infection site is often very low, and so the rapid eradication of the bacterial population could result. we investigated the impact of early versus later curative administrations of 1 or 40 mg/kg ...	2010	20888712
immunolocalization of pulmonary intravascular macrophages, tlr4, tlr9 and il-8 in normal and pasteurella multocida-infected lungs of water buffalo (bubalus bubalis).	water buffalo are of considerable economic significance in south east asia, but these animals suffer from many bacterial respiratory diseases including haemorrhagic septicaemia caused by pasteurella multocida. bacterial respiratory diseases of animals cause lung inflammation that is characterized by the activation of toll-like receptors (tlrs) expressed on macrophages, expression of chemokines and recruitment of neutrophils and monocytes. pulmonary intravascular macrophages (pims) present in the ...	2010	20880542
identification of bacteria associated with feline chronic gingivostomatitis using culture-dependent and culture-independent methods.	feline chronic gingivostomatitis (fcgs) is a chronic inflammatory disease of the oral cavity that causes severe pain and distress. there are currently no specific treatment methods available and little is known regarding its aetiology, although bacteria are thought to play a major role. the purpose of this study was to identify the oral bacterial flora in normal and diseased cats. oral swabs were obtained from the palatoglossal folds of eight cats (three normal and five fcgs) and were subjected ...	2011	20828946
the synthesis of paleic acid, an antimicrobial agent effective against mannheimia and pasteurella, and its structurally related derivatives.	a synthetic route to paleic acid 1, antimicrobial agent effective against mannheimia haemolytica and pasteurella multocida, has been established. the absolute configuration of the secondary hydroxyl group was controlled by a catalytic asymmetric alkylation of an aldehyde using a chiral titanium sulfonamide complex and the cis double bond was installed using a wittig reaction. this synthetic route was also applied to the preparation of structurally related analogs, which were used in structure-ac ...	2010	20728353
disease patterns and immune responses in the offspring to sows with high or low antibody levels to actinobacillus pleuropneumoniae serotype 2.	the serum antibody responses to actinobacillus pleuropneumoniae and the secondary invader pasteurella multocida were monitored from birth until slaughter in the offspring to sows with high or low levels of serum antibodies to a. pleuropneumoniae. serum antibody concentrations to a. pleuropneumoniae were higher from birth to the age of 9weeks in piglets delivered by high responding sows. in contrast, antibody levels to p. multocida were similar in both groups during this period. from the age of 2 ...	2010	20728191
recent insights into pasteurella multocida toxin and other g-protein-modulating bacterial toxins.	over the past few decades, our understanding of the bacterial protein toxins that modulate g proteins has advanced tremendously through extensive biochemical and structural analyses. this article provides an updated survey of the various toxins that target g proteins, ending with a focus on recent mechanistic insights in our understanding of the deamidating toxin family. the dermonecrotic toxin from pasteurella multocida (pmt) was recently added to the list of toxins that disrupt g-protein signa ...	2010	20722598
development of a novel lamp diagnostic method for visible detection of swine pasteurella multocida.	a set of four specific primers for six regions of kmt1 gene from a species specific region was designed for developing the loop-mediated isothermal amplification diagnostic method of swine pasteurella multocida (pm-lamp). after the pm-lamp was carried out at 63°c for 1 h, the lamp products could be visually confirmed using fluorescent dyes as detection reagent under uv-illumination. in sensitivity, the detection limit of the pm-lamp was 10 cfu/ml, and was 1 log less than that of the pcr method. ...	2010	20717843
molecular heterogeneity of plpe gene in indian isolates of pasteurella multocida and expression of recombinant plpe in vaccine strain of p. multocida serotype b: 2.	outer membrane proteins of pasteurella (p.) multocida have been known to be protective immunogens. pasteurella lipoprotein e (plpe) has been reported to be an important cross reactive outer membrane protein in p. multocida. the gene encoding the plpe of p. multocida serotypes a: 3, b: 2 and d: 1 was amplified from the genomic dna. the amplified products were cloned and the nucleotide sequence was determined. sequence analysis of the recombinant clones revealed a single open reading frame of 1,01 ...	2010	20706030
efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of pasteurella multocida b:2 against hemorrhagic septicemia in goats.	this study was carried out to determine the antibody responses and protective capacity of an inactivated recombinant vaccine expressing the fimbrial protein of pasteurella multocida b:2 following intranasal vaccination against hemorrhagic septicemia in goats. goats were vaccinated intranasal with 10(6) cfu/ml of the recombinant vaccine (vaccinated group) and 10(6) cfu/ml of pet32/lic vector without fimbrial protein (control group). all three groups were kept separated before all goats in the thr ...	2011	20697957
longitudinal study of respiratory infection patterns of breeding sows in five farrow-to-finish herds.	a longitudinal study was carried out in five french farrow-to-finish herds differently affected by respiratory diseases to describe the carrying and infection patterns of batches of sows to various respiratory pathogens during gestation and lactation. an entire batch of sows was followed during two successive reproduction cycles. nasal, tonsillar and oro-pharyngeal swabs and blood samples were taken from each sow 9 and 4 weeks before farrowing and 1 and 4 weeks after farrowing. mycoplasma hyopne ...	2011	20696539
microbial communities in the tonsils of healthy pigs.	the tonsils of mammals such as humans and pigs are colonized with an extensive microbiota and are frequently the site for asymptomatic carriage of bacterial pathogens. the goal of this study was to determine the composition of the microbial community of the tonsils in healthy pigs. tonsils were collected from eight pigs from two different healthy herds. samples of the tonsils from each pig were used for culture dependent and culture independent identification of the microbial community. aerobic ...	2011	20663617
molecular epidemiology of japanese avian pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis.	molecular epidemiology analyses of the 36 clinical isolates of pasteurella multocida from various avian hosts in japan between 1976 to 2007 including 5 reference strains from the u.s.a., taiwan and indonesia were performed by employing the single-enzyme amplified fragment length polymorphism (se-aflp) comparison with the classical apai-based pulsed-field gel electrophoresis (pfge). as the results, se-aflp gave 21 profiles while pfge gave 20 profiles. the simpson's index of diversity analysis ind ...	2010	20644339
pasteurella multocida toxin-induced pim-1 expression disrupts suppressor of cytokine signalling (socs)-1 activity.	pasteurella multocida toxin (pmt) is a mitogenic protein toxin that manipulates signal transduction cascades of mammalian host cells and upregulates janus kinase (jak) and signal transducers of transcription (stat) activity. here we show that in the presence of pmt, increased levels of suppressors of cytokine signalling-1 (socs-1) proteins significantly enhance stat activity. this occurs via pmt-induced expression of the serine/threonine kinase pim-1 and subsequent threonine phosphorylation of s ...	2010	20633028
aerobic bacteria from mucous membranes, ear canals, and skin wounds of feral cats in grenada, and the antimicrobial drug susceptibility of major isolates.	in a 2-year period 54 feral cats were captured in grenada, west indies, and a total of 383 samples consisting of swabs from rectum, vagina, ears, eyes, mouth, nose and wounds/abscesses, were cultured for aerobic bacteria and campylobacters. a total of 251 bacterial isolates were obtained, of which 205 were identified to species level and 46 to genus level. a commercial bacterial identification system (api/biomerieux), was used for this purpose. the most common species was escherichia coli (n=60) ...	2011	20627391