Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| cross-reactive adaptive immune response to oral commensal bacteria results in an induction of receptor activator of nuclear factor-kappab ligand (rankl)-dependent periodontal bone resorption in a mouse model. | the present study examined whether induction of an adaptive immune response to orally colonizing non-pathogenic pasteurella pneumotropica by immunization with the phylogenetically closely related bacterium, actinobacillus actinomycetemcomitans, can result in periodontal bone loss in mice. | 2007 | 17488448 |
| crystal structures of pasteurella multocida sialyltransferase complexes with acceptor and donor analogues reveal substrate binding sites and catalytic mechanism. | sialyltransferases are key enzymes involved in the biosynthesis of biologically and pathologically important sialic acid-containing molecules in nature. binary x-ray crystal structures of a multifunctional pasteurella multocida sialyltransferase (delta24pmst1) with a donor analogue cmp-3f(a)neu5ac or cmp-3f(e)neu5ac were determined at 2.0 and 1.9 a resolutions, respectively. ternary x-ray structures of the protein in complex with cmp or a donor analogue cmp-3f(a)neu5ac and an acceptor lactose ha ... | 2007 | 17487984 |
| outer membrane protein h for protective immunity against pasteurella multocida. | pasteurella multocida, a gram-negative facultative anaerobic bacterium, is a causative animal pathogen in porcine atrophic rhinitis and avian fowl cholera. for the development of recombinant subunit vaccine against p. multocida, we cloned and analyzed the gene for outer membrane protein h (omph) from a native strain of pasteurella multocida in korea. the omph had significant similarity in both primary and secondary structure with those of other serotypes. the full-length, and three short fragmen ... | 2007 | 17483806 |
| comparative sequence analysis of 16s rrna gene of pasteurella multocida serogroup b isolates from different animal species. | the phylogenetic relationships of five isolates of pasteurella multocida serotype b:2 belonging to buffalo, cattle, pig, sheep and goat were investigated by comparative sequence analysis of 16s rrna gene. the 1468bp fragment of 16s rrna gene sequence comparison showed that the isolates of cattle (pm75), pig (pm49) and sheep (pm82) shared 99.9% homology with the buffalo isolate (vaccine strain p52) whereas, the goat isolate (pm86) shared 99.8% homology with the vaccine strain. the 16s rrna gene s ... | 2007 | 17459437 |
| successful treatment of cervical spinal epidural empyema secondary to grass awn migration in a cat. | spinal epidural empyema (see) represents a severe pyogenic infection of the epidural space. clinical signs of the disease are non-specific--increased body temperature, intense neck pain, neurological signs of a transverse myelopathy--and can lead to severe and permanent neurological deficits. this report describes the diagnosis and successful surgical treatment of cervical see secondary to grass awn migration in a cat. although it is uncommon, this disease should be suspected in cats with progre ... | 2007 | 17449314 |
| protective immunity conferred by recombinant pasteurella multocida lipoprotein e (plpe). | the genes encoding pasteurella multocida lipoprotein e (plpe) and lipoprotein b (plpb) were cloned from p. multocida strain x-73 (serotype a:1) and expressed in escherichia coli. the protective immunity conferred by recombinant plpe (r-plpe) and plpb (r-plpb) on mice and chickens was evaluated. the results showed that mice immunized with 10microg of purified r-plpe were protected (80-100% survival rate) against challenge infection with 10 or 20 ld(50) of p. multocida strains x-73 (serotype a:1), ... | 2007 | 17449151 |
| pasteurella meningo-encephalitis--a risk of household pets. | 2007 | 17442396 | |
| the tad locus: postcards from the widespread colonization island. | the tad (tight adherence) macromolecular transport system, which is present in many bacterial and archaeal species, represents an ancient and major new subtype of type ii secretion. the tad genes are present on a genomic island named the widespread colonization island (wci), and encode the machinery that is required for the assembly of adhesive flp (fimbrial low-molecular-weight protein) pili. the tad genes are essential for biofilm formation, colonization and pathogenesis in the genera aggregat ... | 2007 | 17435791 |
| ultrastructural characteristics of the external surfaces of pasteurella pneumotropica from mice and pasteurella multocida from rabbits. | the surface structures of the cells of pasteurella pneumotropica from mice and pasteurella multocida from rabbits were examined by transmission electron microscopy after ruthenium red staining and polycationic ferritin labelling. p. pneumotropica strains atcc 35149 and k 79114 had slight extracellular fibrous materials associated with cell walls with ruthenium red staining. ferritin labelling method revealed thick strands or sparsely ferritin-labelled materials on the cell surface of the strains ... | 2007 | 17430628 |
| pasteurella multocida empyema. | 1996 | 17429265 | |
| the effect of route and dosage of immunization on the serological response to a pasteurella haemolytica and haemophilus somnus vaccine in feedlot calves. | the effect of route and dosage of administration on the serological response to a vaccine containing genetically attenuated leukotoxin of pasteurella haemolytica combined with bacterial extracts of p. haemolytica and haemophilus somnus (somnu-star ph, biostar inc., saskatoon, saskatchewan) was evaluated in a controlled field trial in 301 feedlot calves. vaccination of calves on arrival at the feedlot with somnu-star ph significantly (p < 0.05) increased p. haemolytica and h. somnus serum antibod ... | 1993 | 17424338 |
| quebec. isolation of pasteurella caballi in a horse. | 1993 | 17424294 | |
| the effect of subunit or modified live bovine herpesvirus-1 vaccines on the efficacy of a recombinant pasteurella haemolytica vaccine for the prevention of respiratory disease in feedlot calves. | the efficacy of a pasteurella haemolytica vaccine (phv) administered once to calves within 24 hours of arrival at a feedlot was tested for the ability to prevent morbidity and mortality from all bovine respiratory disease (brd) and specifically from fibrinous pneumonia mortality. the phv consisted of two immunizing ingredients: outer membrane proteins extracted from p. haemolytica, plus genetically attenuated leukotoxin produced by recombinant dna technology. this double blind study was conducte ... | 1992 | 17424116 |
| fatal herpesvirus infection in commercial rabbits. | acute mortality occurred in two unrelated rabbitries. in the rabbits examined, an unidentified herpesvirus caused lesions that have not been reported previously in this species. the primary lesions were multifocal hemorrhagic dermatitis on the face and back, localized pneumonia, and severe splenic necrosis. large eosinophilic, intranuclear inclusion bodies that were observed in tissue sections of skin, spleen, and lung were identified as herpes-like viral particles by electron microscopy, and he ... | 1992 | 17424062 |
| a field trial to evaluate the efficacy of a commercial pasteurella haemolytica bacterial extract in preventing bovine respiratory disease. | a double blind, random, controlled field trial was conducted to ascertain the efficacy of a pasteurella haemolytica bacterial extract (presponse, langford inc., guelph, ontario) in the prevention of bovine respiratory disease and/or its effects. calves from 13 ranches (n = 1140 calves) were assigned to one of four groups, namely: vaccinated at the ranch three weeks prior to shipping to the feedlot; vaccinated only on arrival at the feedlot; vaccinated at both locations; or not vaccinated at eith ... | 1990 | 17423645 |
| saskatchewan. association of actinobacillus ureae (pasteurella ureae) with porcine abortion. | 1990 | 17423496 | |
| efficacy of a pasteurella haemolytica vaccine/bacterial extract. | 1989 | 17423278 | |
| efficacy of a pasteurella haemolytica vaccine/bacterial extract in the prevention of bovine respiratory disease in recently shipped feedlot calves. | 1988 | 17423146 | |
| comparison of penicillin, oxytetracycline, and trimethoprim-sulfadoxine in the treatment of acute undifferentiated bovine respiratory disease. | penicillin, oxytetracycline, and a trimethoprimsulfadoxine combination were compared as first choice antibiotics for the treatment of acute bovine respiratory disease in weaned beef calves. there was no statistical difference in the mortality losses due to respiratory disease; however, the case fatality rate in the trimethoprim-sulfadoxine treatment group (3%) was markedly lower than in the penicillin (10%) and oxytetracycline (8%) treatment groups. the trimethoprim-sulfadoxine group also had st ... | 1988 | 17423045 |
| clinical and pathological evaluation of sulbactam/ampicillin for treatment of experimental bovine pneumonic pasteurellosis. | an experiment was conducted to evaluate the efficacy of sulbactam/ampicillin for treatment of bovine pneumonic pasteurellosis. twenty-one hereford calves were experimentally infected with bovine herpesvirus-1 and an ampicillin-resistant strain of pasteurella haemolytica, then treated for three days with either sulbactam/ampicillin, chloramphenicol, or a placebo. the treatments were evaluated by comparing clinical illness scores, total sick days, weight changes, mortality rates, and postmortem lu ... | 1988 | 17422967 |
| efficacy of sulbactam, a b-lactamase inhibitor, combined with ampicillin in the therapy of ampicillin-resistant pneumonic pasteurellosis in veal calves. | two field efficacy studies, involving a total of 92 naturally infected, pneumonic veal calves, were conducted to compare the efficacy of the beta-lactamase inhibitor sulbactam plus ampicillin to ampicillin alone in the treatment of bacterial pneumonia. cultures from nasal swabs and lung tissue during the 10 or 11 day studies were predominantly ampicillin-resistant pasteurella multocida. ampicillin (6.6 mg/kg) or sulbactam-ampicillin (3.3 mg/kg sulbactam + 6.6 mg/kg ampicillin) was injected intra ... | 1987 | 17422867 |
| efficacy of sulbactam, a b-lactamase inhibitor, combined with ampicillin in the therapy of ampicillin-resistant pneumonic pasteurellosis in feedlot calves. | two field efficacy studies, involving a total of 80 naturally infected feedlot calves, were conducted to compare the efficacy of sulbactam-ampicillin with that of penicillin-dihydrostreptomycin in the treatment of pneumonic pasteurellosis. cultures from pretreatment nasal swabs were predominantly ampicillin/ penicillin resistant pasteurella haemolytica. clinical observations revealed that cattle treated with penicillin-dihydrostreptomycin responded poorly, whereas those treated with sulbactam-am ... | 1987 | 17422866 |
| a field trial of preshipment vaccination of calves. | a field trial to investigate the efficacy of vitamins ade, a haemophilus somnus bacterin, a pasteurella bacterin, and two intranasal infectious bovine rhinotracheitis-parainfluenza type 3 vaccines administered to beef calves at least three weeks prior to weaning and shipment was conducted.over 1000 calves were vaccinated, but of the 692 calves shipped from the ranch of origin, only 276 calves were located in ontario, or quebec, feedlots. the average treatment rate was 30%. neither vitamins ade, ... | 1984 | 17422377 |
| therapeutic and prophylactic effects of some antibiotics on experimental pneumonic pasteurellosis. | pneumonic pasteurellosis was produced in cattle seronegative for bovine herpes virus-1 and pasteurella haemolytica using their respective aerosols four days apart. when treated with four daily intravenous oxytetracycline injections one day prior, same day as and 24 hours after p. haemolytica aerosols cattle experienced a reduced mortality. prophylactic sustained action antibiotics given 24 hours prior to the p. haemolytica aerosol also reduced mortality, however there appeared to be a variation ... | 1984 | 17422363 |
| serotypes and antimicrobial susceptibility of pasteurella multocida isolated from cattle and pigs in ontario. | 1984 | 17422356 | |
| an epizootic of swine influenza in ontario. | a swine influenza virus (h(1)n(1)) was isolated for the first time in ontario from pigs one week to one and one-half years old during an epizootic which occurred between january and may 1981. each herd outbreak was characterized by the sudden onset of marked respiratory distress, usually affecting the entire herd, accompanied by paroxysmal coughing, anorexia, prostration and temperatures as high as 41.5 degrees c and lasting for five to seven days. morbidity was nearly 100%; mortality was less t ... | 1983 | 17422266 |
| a discussion of recent developments relating to pasteurella hemolytica with special reference to strains pathogenic for cattle. | 1963 | 17421616 | |
| immunological differentiation of type b and e strains of pasteurella multocida. | 1963 | 17421584 | |
| [bacteriological and virological status in upper respiratory tract infections of cats (cat common cold complex)]. | between october 2002 and january 2005,460 bacteriological samples from cats with an acute upper respiratory tract infection were analysed in clinical field studies in two accredited laboratories in germany. oropharyngeal swabs were taken from these cats and sent to the laboratories for routine diagnostics. in the swab samples of 460 cats 382 bacteria strains were isolated. the following bacteria were isolated most frequently: pasteurella spp. (32.5 %), staphylococcus spp. (18.5 %), escherichia c ... | 2007 | 17416134 |
| one-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities. | chemoenzymatic synthesis, which combines the flexibility of chemical synthesis and the high selectivity of enzymatic synthesis, is a powerful approach to obtain complex carbohydrates. it is a preferred method for synthesizing sialic acid-containing structures, including those with diverse naturally occurring and non-natural sialic acid forms, different sialyl linkages and different glycans that link to the sialic acid. starting from n-acetylmannosamine, mannose or their chemically or enzymatical ... | 2006 | 17406495 |
| reclassification of [pasteurella] trehalosi as bibersteinia trehalosi gen. nov., comb. nov. | [pasteurella] trehalosi is an important pathogen of sheep, being primarily associated with serious systemic infections in lambs but also having an association with pneumonia. the aim of the present investigation was to characterize a broad collection of strains tentatively identified as [p.] trehalosi in order to reclassify and rename this taxon to support improvements in our understanding of the pathogenesis and epidemiology of this important organism. the type strain for [p.] trehalosi, strain ... | 2007 | 17392184 |
| [pasteurella dagmatis: an unusual cause of vertebral osteomyelitis]. | vertebral osteomyelitis is a quite rare but severe cause of back pain in adults. various causative organisms have been reported. pasteurella species have rarely been isolated. we report here a case of vertebral osteomyelitis and consecutive cauda equina syndrome due to pasteurella dagmatis in a 60-year-old diabetic man. | 2007 | 17391865 |
| pasteurella multocida and its role in porcine pneumonia. | pasteurella multocida has been recognized as a contributor to debilitating and fatal porcine pneumonia for at least 120 years and there continues to be sustained, unabated high prevalence of the organism in cases submitted for diagnostic work up. understanding of its role in disease has been limited, in part because of difficulty in reproducing the disease experimentally with capsular type a strains of p. multocida, the predominant type associated with porcine pneumonia. this limitation has stym ... | 2006 | 17389051 |
| experimental transmission of pasteurella multocida 6:b in goats. | haemorrhagic septicaemia (hs) is an acute disease of cattle and buffaloes caused by pasteurella multocida 6:b. outbreaks of the disease have been closely associated with carrier animals that transmit the organism to susceptible animals during stressful condition. this study was conducted to determine whether goats exposed intranasally to p. multocida 6:b can transmit the organism to contact goats. thirty-six healthy local katjang goats were divided into four groups and goats of groups 1 and 3 we ... | 2007 | 17381677 |
| [presence of the microbiological risk in umbrian sawmills]. | the purpose of this study was to quantify and identify the airborne microbial contamination in umbria sawmills. in this paper we reported the preliminary results of our analysis. microbial contaminants (fungi and bacteria) were assessed with passive (ima standard) and active (sas microbial sampler) methods. there were significant differences of bacterial and/or fungal cfu/m3 values between the outdoor and indoor environments during the normal sawmills activity. staphylococcus, sphingomonas, past ... | 2006 | 17380948 |
| effect of ivermectin on the cellular and humoral immune responses of rabbits. | the objective of this paper is to determine the effect of ivermectin administration on cell mediated (cmi) and humoral immunity (hi) of rabbits. cmi against dinitrochlorobenzene (dncb) and sheep red blood cells (srbc) in rabbits was determined by delayed-type hypersensitivity and macrophage engulfment assay (mea), respectively; whereas, hi to pasteurella multocida b2 vaccine and srbc was determined by indirect haemagglutination assay (iha) and jerne hemolytic plaque formation assay (jhpfa), resp ... | 2007 | 17379254 |
| [evaluation of two techniques of molecular subtyping to study pasteurella multocida]. | typeability, reproducibility, and discriminatory power of eric-pcr and apal-pfge to establish the genetic relation of p. multocida strains were determined. forty-nine strains of different source, biotype, capsular group, somatic serotype, and resistance to antimicrobials were studied. by eric-pcr, 31 patterns were defined with 10 to 14 bands in a rank of 0.2 and 1.2 kb. by apal-pfge, 37 restriction patterns were established with 7 to 15 bands of 34 to 450 kb. typeability was 100% (t=1) for eric- ... | 2006 | 17373089 |
| mutations in 16s rrna and ribosomal protein s5 associated with high-level spectinomycin resistance in pasteurella multocida. | pasteurella multocida isolates with high-level spectinomycin resistance in which no adenyltransferase genes could be demonstrated exhibited a c1192g transversion in the 16s rrna of all six or five of the six rrna operons and/or two different types of 3-bp deletions in the rpse gene that codes for the ribosomal protein s5. | 2007 | 17371823 |
| mannheimia haemolytica leukotoxin-induced cytolysis of ovine (ovis aries) leukocytes is mediated by cd18, the beta subunit of beta2-integrins. | mannheimia (pasteurella) haemolytica causes severe pneumonia in cattle, sheep and goats. leukotoxin (lkt) is the most important virulence determinant produced by this organism. previously, we identified cd18, the beta subunit of beta(2)-integrins, as the receptor for lkt on bovine leukocytes. since lkt is specific for leukocytes of cattle, sheep and goats, we hypothesized that lkt utilizes cd18 as its receptor on ovine leukocytes as well. therefore, the objective of this study was to transfect a ... | 2007 | 17368825 |
| fatal pasteurella multocida septicemia and necrotizing fasciitis related with wound licked by a domestic dog. | a 68-y-old male had necrotizing fasciitis and bacteremia due to pasteurella multocida. saliva culture from his dog grew p. multocida and pseudomonas aeruginosa. the human and dog p. multocida strains were of the same antibiogram but not identical tested with ribotyping. the wound licked by his dog was the only risk factor. | 2007 | 17366037 |
| successful treatment of pasteurella multocida meningitis with aztreonam. | this is the first reported case of the successful treatment of pasteurella multocida meningitis with aztreonam in a patient with multiple antibiotic allergies. | 2007 | 17366017 |
| crystal structures reveal a thiol protease-like catalytic triad in the c-terminal region of pasteurella multocida toxin. | pasteurella multocida toxin (pmt), one of the virulence factors produced by the bacteria, exerts its toxicity by up-regulating various signaling cascades downstream of the heterotrimeric gtpases gq and g12/13 in an unknown fashion. here, we present the crystal structure of the c-terminal region (residues 575-1,285) of pmt, which carries an intracellularly active moiety. the overall structure of c-terminal region of pmt displays a trojan horse-like shape, composed of three domains with a "feet"-, ... | 2007 | 17360394 |
| specific identification of gallibacterium by a pcr using primers targeting the 16s rrna and 23s rrna genes. | gallibacterium was recently established as a new genus including organisms previously reported as pasteurella anatis, [actinobacillus] salpingitidis and avian pasteurella haemolytica-like organisms. the aim of the present study was to develop a pcr method allowing unambiguous identification of gallibacterium. pcr primers positioned in the 16s rrna (1133fgal) and 23s rrna (114r) genes were defined and their specificity was subsequently tested on 122 strains. twenty-five of the strains represented ... | 2007 | 17350770 |
| mannheimia (pasteurella) haemolytica leukotoxin utilizes cd18 as its receptor on bighorn sheep leukocytes. | pneumonia caused by mannheimia (pasteurella) haemolytica is a highly fatal disease of bighorn sheep (ovis canadensis). leukotoxin (lkt), secreted by m. haemolytica, is an important virulence factor of this organism, and is cytolytic to bighorn sheep leukocytes. previously, we have shown that cd18, the beta subunit of beta2 integrins, serves as the receptor for lkt on bovine leukocytes. furthermore, anti-cd18 antibodies inhibit lkt-induced cytotoxicity of bighorn sheep leukocytes. therefore, we h ... | 2007 | 17347395 |
| pasteurella multocida detection by 5' taq nuclease assay: a new tool for use in diagnosing fowl cholera. | a 5' taq nuclease assay utilising minor groove binder technology and targeting the 16s rrna gene was designed to detect pasteurella multocida (the causative agent of fowl cholera) in swabs collected from poultry. the assay was first evaluated using pure cultures. the assay correctly identified four p. multocida taxonomic type strains, 18 p. multocida serovar reference strains and 40 australian field isolates (17 from poultry, 11 from pigs and 12 from cattle). representatives of nine other pasteu ... | 2007 | 17346833 |
| a specific polymerase chain reaction based on the gyrb gene sequence and subsequent restriction fragment length polymorphism analysis of pasteurella pneumotropica isolates from laboratory mice. | for a molecular identification and typing tool, we developed a specific polymerase chain reaction (pcr) based on the gyrb gene sequence and a subsequent restriction fragment length polymorphism (rflp) analysis using the products amplified from the specific pcr to facilitate discrimination of biotypes of pasteurella pneumotropica from laboratory mice. appropriate pcr products, a 1039-basepair fragment for biotype jawetz and a 1033-basepair fragment for biotype heyl, were amplified by use of the p ... | 2007 | 17343354 |
| detection of respiratory pathogens in porcine lung tissue and lavage fluid. | the objective of this study was to compare the detection rate of bacterial agents in bronchoalveolar lavage fluid (balf), taken without visual control, to that in affected lung tissue obtained from the same pig at necropsy. balf and affected lung tissue were examined for mycoplasma hyopneumoniae using pcr, and standard cultural methods were used for actinobacillus pleuropneumoniae, bordetella bronchiseptica, haemophilus parasuis, pasteurella multocida and streptococcus suis. all pigs with a hist ... | 2008 | 17339121 |
| pasteurella multocida sepsis, due to a scratch from a pet cat, in a post-chemotherapy neutropenic patient with non-hodgkin lymphoma. | pasteurella (p) multocida exists in a variety of animals and causes diverse infections in humans due to animal bites and scratches, usually by cats or dogs, and oral and respiratory infection. we report a case of p multocida sepsis due to a scratch from a pet cat, complicated with disseminated intravascular coagulation in a post-chemotherapy neutropenic patient with non-hodgkin lymphoma. the patient was a febrile 79-year-old woman with disturbed consciousness and subcutaneous abscess in her righ ... | 2007 | 17321993 |
| communicable disease and health protection quarterly review: july to september 2006. from the health protection agency. | 2007 | 17311831 | |
| [pneumonia with septicemia caused by pasteurella multocida in an immunocompetent patient]. | pneumonia with septicemia caused by pasteurella multocida was diagnosed in an immunocompetent patient exposed to a dog. this case is remarkable by two aspects: first the absence of visible cutaneous lesion, and second the localization and severity of the infection caused by p. multocida even though the patient was immunocompetent. p. multocida can cause respiratory and systemic infection, and it is a possible diagnosis in case of exposure to animals, even without history of bite or scratch. furt ... | 2007 | 17303362 |
| [influence of cultivation time and composition of growth medium on formation of capsule, quantity and immunological properties of pasteurella multocida capsular antigen]. | results of study of capsule formation and immunologic properties of capsular antigen of attenuated strain la-25 of pasteurella multocida grown on liquid medium from non-food materials and hottinger's broth depending on cultivation time are presented. | 2006 | 17297891 |
| isolated endocarditis of the pulmonary valve caused by pasteurella multocida. | we describe the rare case of a patient with an isolated endocarditis of the pulmonary valve caused by pasteurella multocida. the bacterium was cultured from blood as well as from the excised valve after pulmonary valve replacement. risk factors were contact with animals and concurrent intravenous drug use. | 2007 | 17297591 |
| differentiation of avian pasteurella multocida strains by single-primer pcr. | 2007 | 17294263 | |
| evaluation of subgingival bacteria in the dog and susceptibility to commonly used antibiotics. | the aim of the present investigation was to evaluate the subgingival aerobic and anaerobic flora of 13 dogs with periodontal disease and the susceptibility of these bacteria to antibiotics currently approved in italy for treatment of canine infections. of the anaerobic bacteria, bacteroides fragilis was most frequently isolated, followed by peptostreptococcus + porphyromonas gingivalis and prevotella intermedia. of the aerobic bacteria, alpha-hemolytic streptococcus was most frequently isolated, ... | 2006 | 17286127 |
| quantification of mannheimia haemolytica leukotoxin by indirect elisa. | an indirect enzyme-linked immunosorbent assay (elisa) was developed for the quantification of extracellular leukotoxin (lkt) produced in chemostat culture of mannheimia haemolytica in a serum-free culture medium. leukotoxin purified with preparative sds-page was used for the production of chicken polyclonal antibodies (pab) that served as the primary detecting antibody. excising the lkt protein from an analytical sds-page gel proved an efficient technique for the purification of the toxin. conse ... | 2006 | 17283723 |
| cloning and characterisation of the ahpa gene of pasteurella multocida serogroup b:2 (strain p52): short communication. | pasteurella multocida b:2 is responsible for haemorrhagic septicaemia in cattle and buffaloes, causing severe economic losses in the developing countries. in the present study, the ahpa gene of p. multocida b:2 (p52) was cloned, sequenced and compared with the previously reported ahpa gene sequence in p. multocida a:1, which is responsible for its haemolytic phenotype. e. coli dh5a cells were further transformed with recombinant plasmid carrying the ahpa gene from p. multocida b:2 (p52) but sds- ... | 2006 | 17278717 |
| efficacy of haemorrhagic septicaemia alum-precipitated vaccine. | 2007 | 17277309 | |
| protective effect following intranasal exposure of goats to live pasteurella multocida b:2. | this study aimed to determine the effect of intranasal exposure to low doses of pasteurella multocida b:2 on survival of goats challenged with high doses of the same organism. eighteen goats were selected and divided into three groups. goats of group 1 were exposed intranasally twice, with a two-week interval, to 7 x 10(6) cfu/ml of live p. multocida b:2. goats of group 2 were not exposed to p. multocida b:2 but were kept together with the exposed group 1. goats of group 3 remained as unexposed ... | 2006 | 17265769 |
| acute tissue-type plasminogen activator release in human microvascular endothelial cells: the roles of galphaq, plc-beta, ip3 and 5,6-epoxyeicosatrienoic acid. | the acute physiologic release of tissue-type plasminogen activator (t-pa) from the endothelium is critical for vascular homeostasis. this process is prostacyclin- and nitric oxide (no)-independent in humans. it has been suggested that calcium signaling and endothelial-derived hyperpolarizing factors (edhf) may play a role in t-pa release. g-protein-coupled receptor-dependent calcium signaling is typically galphaq-dependent. edhfs have been functionally defined and in various tissues are believed ... | 2007 | 17264956 |
| using capacitance measurements as the detection method in antigen-containing layer-by-layer films for biosensing. | the layer-by-layer technique is employed here to immobilize antigen-containing liposomes, so-called proteoliposomes, onto au-interdigitated substrates, which are capable of molecular recognition of anti-pasteurellosis antibodies. detection was carried out using a novel strategy entirely based upon capacitance measurements, and to enhance sensitivity, we combine the response of three different sensing units in a similar procedure used for taste sensors. with the three-electrode array immunoglobul ... | 2007 | 17263514 |
| pasteurella multocida urinary tract infection in a pediatric patient with end-stage renal disease. | pasteurella multocida is a gram-negative bacillus that is part of the normal oral flora of cats and dogs. most infections involving p. multocida are soft tissue infections after animal bites or scratches. we present a case of p. multocida urinary tract infection in a 13-year-old boy with end-stage renal disease receiving peritoneal dialysis. he was successfully treated with intravenous ampicillin-sulbactam followed by oral amoxicillin-clavulanate. thirteen additional cases of p. multocida urinar ... | 2007 | 17259887 |
| comparative functional genomic analysis of pasteurellaceae adhesins using phage display. | the pasteurellaceae contain a number of important animal pathogens. although related, the various members of this family cause a diversity of pathology in a wide variety of organ systems. adhesion is an important virulence factor in bacterial infections. surprisingly little is known about the adhesins of the pasteurellaceae. to attempt to identify the genes coding for adhesins to some key components of the hosts extracellular matrix molecules, phage display libraries of fragmented genomic dna fr ... | 2007 | 17258409 |
| association of bovine respiratory disease with clinical status and acute phase proteins in calves. | eighty-four calves with respiratory disease from 18 herds in different parts of finland were chosen for a study evaluating the capacity of different respiratory pathogens to cause changes in different acute phase protein concentrations, white blood cell (wbc) count and clinical signs. the selected acute phase proteins were fibrinogen, haptoglobin, serum amyloid-a, lipopolysaccharide binding protein and alpha1-acid glycoprotein. from each calf, a paired blood sample was obtained for serological s ... | 2007 | 17258318 |
| development of a novel pcr assay specific for riemerella anatipestifer. | riemerella anatipestifer is a significant pathogen of waterfowl and turkeys. due to their similar ecology and morphological and cultural characteristics it is important to differentiate r. anatipestifer infections from those caused by pasteurella multocida. present study describes a novel pcr assay that is capable of rapid and species-specific identification of r. anatipestifer from bacterial cultures. | 2007 | 17257252 |
| cytochemical and ultrastructural study of anoikis and secondary necrosis in enterocytes detached in vivo. | detachment-induced apoptosis of enterocytes (anoikis) has not been investigated in vivo. here we describe anoikis of fish enterocytes following detachment in a septicemia by photobacterium damselae subsp. piscicida, or following injection of its exotoxin. the in vivo study was complemented with an ex vivo time-lapse analysis using conditions duplicating the in vivo situation. linings of enterocytes detached from intestine mucosa dissociate into isolated enterocytes which undergo caspase 3-mediat ... | 2007 | 17245641 |
| the response of mannheimia haemolytica to iron limitation: implications for the acquisition of iron in the bovine lung. | mannheimia haemolytica is the major causative agent of shipping fever, a severe pneumonia in cattle causing high morbidity and mortality. a prerequisite of successful lung colonization by m. haemolytica is the necessity to adapt to the paucity of iron. the lack of genome information has precluded an assessment of the genetic repertoire available to m. haemolytica to adapt to low iron environments. to close this knowledge-gap, we have determined 90% of a virulent m. haemolytica serotype a1 genome ... | 2007 | 17240088 |
| identification of novel immunogens in pasteurella multocida. | p. multocida is a gram-negative pathogen responsible for causing diseases in animals of economic significance to livestock industries throughout the world. current vaccines include bacterins, which provide only limited protection against homologous serotypes. therefore there is a need for more effective vaccines to control diseases caused by p. multocida. as a step towards developing vaccines against fowl cholera, a genomics based approach was applied for the identification of novel immunogens. ... | 2007 | 17233917 |
| [cat scratch pneumonia: a case report]. | pasteurella multocida infections can be caused by scratches or bites of many animals including pets. an unrecognized infection can lead to severe complications such as arthritis, osteomyelitis, respiratory infections and even meningitis or endocarditis. we present the case of a woman affected by soft tissue infection caused by pasteurella multocida complicated by bacteremia and pneumonia. | 2006 | 17228851 |
| cpg oligodeoxynucleotides augment the immune responses of piglets to swine pasteurella multocida living vaccine in vivo. | oligodeoxynucleotides containing unmethylated cpg motifs (cpg odn) prevent development of t-helper type 2 (th2) immune response and reverse established allergic responses in mouse models. however, little work on immune responses in piglets has been conducted in vivo. in this report, the ability of a porcine-specific cpg odn to act as an immunostimulant and enhance immune responses of piglets to swine pasteurella multocida living vaccine (spml vaccine) was determined. the titre of igg and igg1/ig ... | 2007 | 17223146 |
| cloning and characterization of type 4 fimbrial gene (ptfa) of pasteurella multocida serogroup b:2 (strain p52). | 2007 | 17216317 | |
| [pasteurella multocida as a rare cause of neonatal meningitis]. | pasteurella multocida (p. multocida), a gram-negative bacillus is a typical commensal in the oropharynx of animals such as dogs and cats. human diseases caused by p. multocida are rare and include respiratory infections, sepsis and meningitis. p. multocida infections are described predominantly in patients with underlying chronic disorders, in elderly patients or in infants < or = 1 year. we describe the case of a 3-week-old boy with meningitis due to pasteurella multocida. p. multocida was also ... | 2006 | 17206557 |
| quantitative continuous assay for hyaluronan synthase. | a rapid, continuous, and convenient three-enzyme coupled uv absorption assay was developed to quantitate the glucuronic acid and n-acetylglucosamine transferase activities of hyaluronan synthase from pasteurella multocida (pmhas). activity was measured by coupling the udp produced from the pmhas-catalyzed transfer of udp-glcnac and udp-glcua to a hyaluronic acid tetrasaccharide primer with the oxidation of nadh. using a fluorescently labeled primer, the products were characterized by gel electro ... | 2007 | 17173853 |
| new consensus guidelines from the clinical and laboratory standards institute for antimicrobial susceptibility testing of infrequently isolated or fastidious bacteria. | the clinical and laboratory standards institute (clsi) recently published a new laboratory guideline for antimicrobial susceptibility testing of infrequently encountered or fastidious bacteria not covered in previous clsi publications. the organisms include aeromonas species, bacillus species, and vibrio species that may cause infections following environmental exposure. fastidious organisms that may cause endocarditis or medical device infections include abiotrophia and granulicatella species; ... | 2007 | 17173232 |
| cloning of the gene and characterization of the enzymatic properties of the monomeric alkaline phosphatase (phox) from pasteurella multocida strain x-73. | we have identified a new phox gene encoding the monomeric alkaline phosphatase from pasteurella multocida x-73. this gene was not found in the published genome sequence of pasteurella multocida pm70. characterization of the recombinant phox of pasteurella multocida x-73 showed that it is a monomeric enzyme, activated by ca(2+) and possibly secreted by the tat pathway. these features distinguish phosphatases of the phox family from those of the phoa family. all proteins of the phox family were fo ... | 2007 | 17156125 |
| [identification, biotypification and characterization of pasteurella multocida strains isolated in argentina]. | thirty pasteurella multocida strains isolated in argentina from human and animal samples were identified, biotypified and characterized. twenty-two (73%) strains were identified as p. multocida subsp. multocida, 5 (17%) as p. multocida subsp. gallicida, and 3 (10%) as p. multocida subsp. septica. all strains were grouped in 8 biotypes, and 70% of the strains presented capsular type a. the most frequent somatic serotypes were 1 (n:11) and 3 (n:9). p. multocida strains from swine source were resis ... | 2006 | 17152652 |
| modulation of host cell gene expression through activation of stat transcription factors by pasteurella multocida toxin. | the pasteurella multocida toxin (pmt) is highly mitogenic and has potential carcinogenic properties. pmt causes porcine atrophic rhinitis that is characterized by bone resorption and loss of nasal turbinates, but experimental nasal infection also leads to excess proliferation of bladder epithelial cells. pmt acts intracellularly and activates phospholipase c-linked signals and mapk pathways via the heterotrimeric galpha(q) and galpha(12/13) proteins. we found that pmt induces activation of stat ... | 2007 | 17150962 |
| rep-pcr analysis of pasteurella multocida isolates from wild and domestic animals in india. | repetitive extragenic palindromic sequence-based pcr (rep-pcr) was used to characterize 67 field isolates of pasteurella multocida originating from different animal species and geographical regions of india. rep-pcr was found to be rapid and reproducible (three repeats were done). these isolates yielded different 23 profiles which were clustered into eight groups. the discrimination index was moderate (d value 0.83). somatic and antigenic typing of the isolates did not reveal any correlation wit ... | 2006 | 17139535 |
| seppic vaccine adjuvants for poultry. | two inactivated antigens (newcastle and pasteurella multocida) were formulated with different adjuvants and tested in two separate experiments in poultry. oil formulations constituting water in oil (w/o) or water in oil in water (w/o/w) emulsions were assessed for antibody response, protection, local reactions, and vaccine physicochemical parameters. robust, efficacious, and safe formulations were obtained with w/o formulations whereas w/o/w was especially safe with maintained efficacy. results ... | 2006 | 17135512 |
| synthesis and biological study of medicinally important mannich bases derived from 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a pentahydroxy naphthacene carboxamide. | the paper describes synthesis and antibacterial study of biologically active mannich bases of carboxamide derivative employing mannich reaction of 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a pentahydroxy naphthacene carboxamide with various sulfonamides/secondary amines . they were analysed by elemental analysis and characterized by uv, ir and (1)h nmr spectroscopic studies. the mannich bases were screened for antibacterial activity against various gram-negative bacteria at va ... | 2007 | 17110110 |
| pasteurella multocida pathogenesis: 125 years after pasteur. | pasteurella multocida was first shown to be the causative agent of fowl cholera by louis pasteur in 1881. since then, this gram-negative bacterium has been identified as the causative agent of many other economically important diseases in a wide range of hosts. the mechanisms by which these bacteria can invade the mucosa, evade innate immunity and cause systemic disease are slowly being elucidated. key virulence factors identified to date include capsule and lipopolysaccharide. the capsule is cl ... | 2006 | 17107417 |
| immunization with outer membrane proteins of pasteurella multocida (6:b) provides protection in mice. | the immunoprotective efficacy of pasteurella multocida (6:b) outer membrane proteins (omps) was examined in the mouse model. bacterial omps were extracted using sarkosyl method and analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting. prototype vaccines were prepared using omps with adjuvants including dioleoyl phosphatidyl choline-based liposome and montanide isa206 water-in oil-in water emulsion. antibody response to the vaccine was monitored using indirect ... | 2006 | 17105574 |
| endotoxin and mammalian host responses during experimental disease. | endotoxin is an integral component of the outer membrane of gram-negative bacteria and a prime example of unique and highly conserved bacterial surface molecules that engage with the innate immune system of the mammalian host via pattern recognition receptors on a range of host cells. the results of this interaction, which may be beneficial or detrimental to the development and welfare of the host, are reviewed, focusing on the different sensitivities and consequences in a range of hosts of expe ... | 2006 | 17101336 |
| acceptor specificity of the pasteurella hyaluronan and chondroitin synthases and production of chimeric glycosaminoglycans. | the hyaluronan (ha) synthase, pmhas, and the chondroitin synthase, pmcs, from the gram-negative bacterium pasteurella multocida polymerize the glycosaminoglycan (gag) sugar chains ha or chondroitin, respectively. the recombinant escherichia coli-derived enzymes were shown previously to elongate exogenously supplied oligosaccharides of their cognate gag (e.g. ha elongated by pmhas). here we show that oligosaccharides and polysaccharides of certain noncognate gags (including sulfated and iduronic ... | 2007 | 17099217 |
| full genome sequence and some biological properties of reticuloendotheliosis virus strain apc-566 isolated from endangered attwater's prairie chickens. | reticuloendotheliosis virus (rev) causes runting, high mortality, immunosuppression, and chronic neoplasia associated with t and/or b cell lymphomas in a variety of domestic and wild birds, including attwater's prairie chickens (apc) (tympanuchus cupido attwateri). the complete proviral sequence of a recent rev isolate from apc (rev apc-566) was determined. this virus was isolated from an apc maintained in captivity in a reproduction program intended to avoid its extinction. rev apc-566 was dete ... | 2007 | 17098316 |
| the functional molecular mass of the pasteurella hyaluronan synthase is a monomer. | hyaluronan (ha), a linear polysaccharide composed of beta1,3-glcnac-beta1,4-glcua repeats, is found in the extracellular matrix of vertebrate tissues as well as the capsule of several pathogenic bacteria. all known ha synthases (hass) are dual-action glycosyltransferases that catalyze the addition of two different sugars from udp-linked precursors to the growing ha chain. the bacterial hyaluronan synthase, pmhas from gram-negative pasteurella multocida, is a 972-residue membrane-associated prote ... | 2007 | 17095162 |
| phylogenetic analysis of isolates of pasteurella pneumotropica from laboratory animals based on the gyrb gene sequence. | phylogenetic analysis using the gyrb sequence was performed to investigate the genetic relevance among 49 isolates of p. pneumotropica. in the phylogeny, the isolates were clearly classified into three groups as follows: group a for the isolates of biotype jawetz derived from mice, group b for the isolates of biotype jawetz derived from rats, and group c for the isolates of biotype heyl. these results suggest that the gyrb sequence of p. pneumotropica differs between the isolates of two biotypes ... | 2006 | 17090967 |
| etiology of respiratory disease in non-vaccinated, non-medicated calves in rearing herds. | the aim of this study was to examine the occurrence of bacterial, mycoplasmal and viral pathogens in the lower respiratory tract of calves in all-in all-out calf-rearing units. according to clinical status, non-medicated calves with and without respiratory disease signs were selected of the 40 herds investigated to analyse the micro-organisms present in healthy and diseased calves. tracheobronchial lavage (tbl) and paired serum samples were analysed for bacteria, mycoplasmas, respiratory syncyti ... | 2007 | 17084565 |
| pulmonary disposition of tilmicosin in foals and in vitro activity against rhodococcus equi and other common equine bacterial pathogens. | the objectives of this study were to determine the serum and pulmonary disposition of tilmicosin in foals and to investigate the in vitro activity of the drug against rhodococcus equi and other common bacterial pathogens of horses. a single dose of a new fatty acid salt formulation of tilmicosin (10 mg/kg of body weight) was administered to seven healthy 5- to 8-week-old foals by the intramuscular route. concentrations of tilmicosin were measured in serum, lung tissue, pulmonary epithelial linin ... | 2006 | 17083461 |
| pharmacokinetics of cefovecin in cats. | the pharmacokinetics of the novel cephalosporin cefovecin were investigated in a series of in vivo, ex vivo and in vitro studies following administration to adult cats at 8 mg/kg bodyweight. bioavailability and pharmacokinetic parameters were determined in a cross-over study after intravenous (i.v.) and subcutaneous (s.c.) injections. [14c]cefovecin was used to evaluate excretion for 21 days after s.c. administration. protein binding was determined in vitro in feline plasma and ex vivo in transu ... | 2006 | 17083455 |
| pasteurella multocida septicemia in a newborn without scratches, licks or bites. | 2006 | 17058424 | |
| an unusual cause of sepsis and meningitis in a neonate. | 2006 | 17055368 | |
| intrapleural heparin or heparin combined with human recombinant dnase is not effective in the treatment of empyema in a rabbit model. | the aim of this study was to investigate the effectiveness of intrapleural heparin or heparin combined with human recombinant dnase in the treatment of empyema. | 2006 | 17052304 |
| neonatal septicemia due to pasteurella multocida: the first case report in thailand. | neonatal septicemia acquired by vertical transmission of pasteurella multocida is very rare. the authors report a case of pasteurella multocida septicemia in a 2-day-old male infant. his mother had a history of prolonged premature rupture of membranes and subsequently developed fever. the patient had fever and lethargy at 36 hours of age, then developed severe pneumonia, sepsis, persistent pulmonary hypertension, renal failure and liver failure. although the appropriate antibiotics were given, h ... | 2006 | 17048443 |
| antimicrobial susceptibility patterns of respiratory tract pathogens from sheep and goats. | to determine the antimicrobial susceptibility of common respiratory tract pathogens from sheep and goats. | 2006 | 17042733 |
| evolution of competence and dna uptake specificity in the pasteurellaceae. | many bacteria can take up dna, but the evolutionary history and function of natural competence and transformation remain obscure. the sporadic distribution of competence suggests it is frequently lost and/or gained, but this has not been examined in an explicitly phylogenetic context. additional insight may come from the sequence specificity of uptake by species such as haemophilus influenzae, where a 9 bp uptake signal sequence (uss) repeat is both highly overrepresented in the genome and neede ... | 2006 | 17038178 |
| etiological agents of dairy cows' mastitis in western part of poland. | the purpose of the study was to determine the etiological mastitis agents in cows from herds located in the western part of poland in years 2003-2005. bacteriological examinations of 18,713 samples taken from california mastitis test (cmt) positive quarters were performed according to standard methods. some gram-negative bacilli and gram-positive cocci were examined with the use of api tests (analytical profile index). it was found that 32.7% of samples that were cmt positive were culture negati ... | 2006 | 17020014 |
| detection of tetracycline-resistant and susceptible pasteurellaceae in the nasopharynx of loose group-housed calves. | the aim of the present study was to determine which pasteurella and mannheimia species are present in the upper respiratory tract of healthy calves with no history of antimicrobial treatment prior to sampling. the presence of subpopulations of tetracycline-resistant pasteurellaceae was also investigated. nasal swabs from 61 loose group-housed, clinically healthy calves, 1 to 4 months old, from 16 dairy herds were inoculated aerobically on a selective medium (columbia agar with 5% ovine blood and ... | 2006 | 17004035 |
| antimicrobial activity of cathelicidins bmap28, smap28, smap29, and pmap23 against pasteurella multocida is more broad-spectrum than host species specific. | the antimicrobial activity of linear, cationic alpha-helical peptides from cattle (bmap28), sheep (smap28 and smap29), and pigs (pmap23) were assessed to determine if activity was selective for pasteurella multocida from a particular animal species or broad-spectrum against all p. multocida tested. the antimicrobial activities of synthetic peptides were determined for p. multocida isolated from cattle (10 isolates), sheep (10 isolates), and pigs (10 isolates) in a broth microdilution assay. all ... | 2007 | 16997510 |
| complications of a cat bite. | 2006 | 16988179 | |
| [pasteurella dagmatis spondylodiscitis in a diabetic patient]. | healthy pets contact are able to induce unusual and severe diseases. | 2006 | 16978746 |