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microbial aetiologic agents associated with pneumonia in immunocompromised hosts.pulmonary infections are a major cause of morbidity and mortality in the immunosuppressed patients. the aim of this study was to determine the etiologic agents and predisposing factors associated with pneumonia infections in immunocompromised patients. cross-sectional survey of 100 immunocompromised patients due to hiv and mycobacterium tuberculosis infections were enlisted for the study. the patients completed a structured questionnaire to abstract information on demographic features and risk f ...201023878695
epidemiology and bacterial colonization of burn injuries in blantyre.forty-nine patients from the burns unit at the qech had swabs taken from various sites in order to determine the bacterial profile and antibiotic susceptibilities in burn wounds colonized by bacteria. the mean age was 16 years (range 1-70 years); 27 (55 %) of the study population were female and 22 (45%) were male. twenty-four (49%) patients were epileptic. open fire (41%) was the most common cause of burn injuries among epileptics while hot water burns (29%) were commonest among non-epileptics. ...200723878628
anti-proteus activity of some south african medicinal plants: their potential for the prevention of rheumatoid arthritis.a wide variety of herbal remedies are used in traditional african medicine to treat rheumatoid arthritis (ra) and inflammation. thirty-four extracts from 13 south african plant species with a history of ethnobotanical usage in the treatment of inflammation were investigated for their ability to control two microbial triggers for ra (proteus mirabilis and proteus vulgaris). twenty-nine of the extracts (85.3 %) inhibited the growth of p. mirabilis and 23 of them tested (67.7 %) inhibited the growt ...201423877712
extended-spectrum β-lactamase- and ampc-producing enterobacteria in healthy broiler chickens, germany.during 2010, we evaluated the presence of extended-spectrum β-lactamase- and ampc-producing enterobacteria in broiler chickens at slaughter. samples (70 carcasses and 51 ceca) from 4 flocks were analyzed by direct plating and after enrichment. extended-spectrum β-lactamase producers were found in 88.6% and 72.5% of carcasses and ceca, respectively; ampc producers were found in 52.9% and 56.9% of carcasses and ceca, respectively. most isolates were identified as escherichia coli; enterobacter clo ...201323876576
emergence of new delhi metallo-β-lactamase 1 (ndm-1) producing and multidrug resistant uropathogens causing urinary tract infections in andaman islands, india.the present study was undertaken to understand the distribution and the antimicrobial susceptibility patterns of the uropathogens in the remote islands of india. patients' demographical data, urine culture results, and resistance rates were analyzed and multidrug resistant isolates were characterized. significant bacteriuria was found in 16 (17.7%, 95% confidence interval [ci] 8.47-23.53%) samples, which includes escherichia coli 9 (56.3%); klebsiella pneumoniae 3 (18.8%); enterobacter sp. 2 (12 ...201323862639
convenient syntheses of cyanuric chloride-derived nhc ligands, their ag(i) and au(i) complexes and antimicrobial activity.convenient syntheses of mono- and bis-imidazolium 1,3,5-triazine derivatives bearing piperidine and morpholine substituents are reported. in situ deprotonation of the mono-imidazolium salts and reaction with ag2o or au(tht)cl (tht = tetrahydrothiophene) precursors affords the corresponding ag(nhc)cl and au(nhc)cl carbene complexes. in the presence of ag(i) or au(i) salts the bis-imidazolium pincers eliminate the imidazolium group to afford -ome or -nme2 substituted triazines depending on the sol ...201323856977
"distribution and functional identification of complex class 1 integrons".the emergence of extended-spectrum β-lactamases and plasmid-mediated resistance to quinolones has been previously found to be associated with the dissemination of complex class 1 integrons in argentina. in this study, we analyzed their distribution through time and evaluated the functionality of the orf513 protein, which is the putative recombinase of the iscr1 mobile element. we investigated the presence of the orf513, blactx-m-2, dfra3b, qnrb10 and bladha-1 genes by pcr and dna sequencing as w ...201323838285
adverse effects of members of the enterobacteriaceae family on boar sperm quality.semen samples collected in 2012 from 1785 boars belonging to five different breeds were recruited from the quality control laboratory of magapor sl, spain. these samples came from 43 boar studs and resulted from diluting the ejaculates in commercial semen extenders. evaluation of the semen sample characteristics (color, smell, ph, osmolality, concentration, motility of sperm cells, agglutination, acrosome integrity, short hypoosmotic swelling test, and abnormal forms) revealed that they met the ...201323827823
microorganisms' colonization and their antibiotic resistance pattern in oro - tracheal tube.recently, nosocomial infections have been discussed as a critical issue among intubated patients leading to significant morbidity and mortality. hence, the pattern of microbiological colonization and antibiotic resistance are much valuable in this regard. we aimed to investigate the pattern of microorganism colonization and antibiotic resistance in patients with endotracheal tube or tracheostomy to propose a proper empirical antibiotic therapy in this setting.201323825725
a novel c-type lysozyme from mytilus galloprovincialis: insight into innate immunity and molecular evolution of invertebrate c-type lysozymes.a c-type lysozyme (named as mgclyz) gene was cloned from the mussel mytilus galloprovincialis. blast analysis indicated that mgclyz was a salivary c-type lysozyme which was mainly found in insects. the nucleotide sequence of mgclyz was predicted to encode a polypeptide of 154 amino acid residues with the signal peptide comprising the first 24 residues. the deduced mature peptide of mgclyz was of a calculated molecular weight of 14.4 kd and a theoretical isoelectric point (pi) of 8.08. evolution ...201323818979
cytotoxic, antioxidant, antimicrobial properties and chemical composition of rose petals.rosa rugosa petals are used for production of teas, jams, wines and juices. despite the wide availability of rose cultivars, comprehensive information on petal chemical composition and healthful properties is still lacking. therefore, the aim of this study was analysis of cytotoxic, antioxidant and antimicrobial activity and chemical composition of rugosa rose petals.201423818393
innate immune responses to proteus mirabilis flagellin in the urinary tract.flagella are bacterial virulence factors allowing microorganisms to move over surfaces. flagellin, the structural component of flagella, is sensed by the host via toll and nod-like receptors and triggers pro-inflammatory responses. the use of toll-like receptors agonists to modulate innate immune responses has aroused great interest as an alternative to improve the treatment of diverse infectious diseases. proteus mirabilis is a gram negative bacterium that causes urinary tract infections in hum ...201623817034
community-acquired urinary tract infection: age and gender-dependent etiology.choosing the antimicrobial agent for initial therapy of urinary tract infection (uti) is usually empirical and should consider the prevalence of uropathogens in different age groups and gender.201323812565
double trouble: preseptal cellulitis due to two species with multidrug resistance.a 2 year old boy presented with painful ballooning of both eyes with the 2 days history of trauma to the head while playing. his vaccination was complete. on examination he was afebrile. the eyes were ballooned with blackish crust over both lids. on local examination, eye swelling was tense with severe tenderness. the diagnosis of preseptal cellulitis was made .we did an emergency drainage and pus was sent for culture that came out to be positive for pseudomonas aeruginosa and proteus mirabilis ...201323803519
chronic otorrhoea: spectrum of microorganisms and antibiotic sensitivity in a south african cohort.chronic otorrhoea is difficult to treat, with treatment in south africa (sa) being protocol driven and generally initiated at the primary healthcare level. there is a lack of local studies that focus on the bacteriology and antimicrobial sensitivities of chronic otorrhoea, which underpins the management advice offered.201323802211
effects of ceftazidime and ciprofloxacin on biofilm formation in proteus mirabilis rods.proteus mirabilis rods are one of the most commonly isolated species of the proteus genus from human infections, mainly those from the urinary tract and wounds. they are often related to biofilm structure formation. the bacterial cells of the biofilm are less susceptible to routinely used antimicrobials, making the treatment more difficult. the aim of this study was to evaluate quantitatively the influence of ceftazidime and ciprofloxacin on biofilm formation on the polyvinyl chloride surface by ...201323801185
antimicrobial activity of willowherb (epilobium angustifolium l.) leaves and flowers.since the aetiology of benign prostatic hyperplasia (bhp) is still unknown, the use of medicinal herb extracts and products prepared thereof are recommended due to their antimicrobial activity, especially during early stages of bhp. a comparison was performed of the in vitro antimicrobial activity (using broth microdilution assay) of flowers and leaves of willowherb (epilobium angustifolium l., onagraceae) from mt. velebit (croatia). the strains (standard atcc and clinical isolates) of staphyloc ...201323796429
genome sequence of proteus mirabilis strain pr03, isolated from a local hospital in malaysia.proteus mirabilis is one of the pathogenic agents that commonly causes urinary tract infections among elderly individuals and long-term catheterized patients. here, we report a draft genome sequence of proteus mirabilis strain pr03 (3,932,623 bp, with a g+c content of 38.6%) isolated from a local hospital in malaysia.201323792750
synthesis and biological evaluation of some novel thiazole compounds as potential anti-inflammatory agents.in the present investigation, furo[2,3-d]thiazol-5(2h)-one 5 was obtained from reaction of thiosemicarbazone derivative 2 with diethyl acetylene dicarboxylate. a series of newly synthesized 2-(hydrazinyl)thiazol-4(5h)-one 6, 7 &8 and 2-(4-(substituted)-thiazol-2-yl)hydrazono derivatives 9a, b &10 were synthesized from treatment of thiosemicarbazone derivative 2 with appropriate α-halogenated compounds. also, a one pot synthesis of thiazole derivatives 13 &15 was achieved from three components re ...201323787438
in vitro assessment of antioxidant and antibacterial activities of six edible plants from iran.the antioxidant and antibacterial activities of six edible plants were evaluated. the active constituents of the edible plants were extracted using boiling water or 80% methanol. results demonstrated that extraction of antioxidants by boiling water was more efficient. lycium depressum and berula angustifolia had the highest antioxidant activities and, therefore, could be rich sources of natural antioxidants. the antibacterial activities of the extracts were tested against staphylococcus aureus, ...201323787285
macromolecular oxidation in planktonic population and biofilms of proteus mirabilis exposed to ciprofloxacin.diverse chemical and physical agents can alter cellular functions associated with the oxidative metabolism, thus stimulating the production of reactive oxygen species (ros). proteins and lipids may be important targets of oxidation, and this may alter their functions in planktonic bacterial physiology. however, more research is necessary to determine the precise role of cellular stress and macromolecular oxidation in biofilms. the present study was designed to evaluate whether ciprofloxacin (cip ...201423771722
chemical composition, antimicrobial properties and toxicity evaluation of the essential oil of cupressus lusitanica mill. leaves from cameroon.the leaves of cupressus lusitanica mill. are used in the western highlands of cameroon for their medicinal property.201323758765
cranberry impairs selected behaviors essential for virulence in proteus mirabilis hi4320.proteus mirabilis is an etiological agent of complicated urinary tract infections. north american cranberries (vaccinium macrocarpon) have long been considered to have protective properties against urinary tract infections. this work reports the effects of cranberry powder (cp) on the motility of p. mirabilis hi4320 and its expression of flaa, flhd, and ured. our results show that swimming and swarming motilities and swarmer-cell differentiation were inhibited by cp. additionally, transcription ...201323750959
prevalence of quinolone resistance determinant qnra6 among broad- and extended-spectrum beta-lactam-resistant proteus mirabilis and morganella morganii clinical isolates with sul1-type class 1 integron association in a tunisian hospital.the aim of this study was to investigate the prevalence and the emergence of plasmid-mediated quinolone resistance among broad-spectrum beta-lactam-resistant proteus mirabilis and morganella morganii clinical isolates recovered in the military hospital in tunisia.201323746343
comparison of double disk synergy test, vitek 2 and check-mdr ct102 for detection of esbl producing isolates.this study is to define the statistical significance for detection of esbl producers by the double disk synergy test and molecular test (check-mdr ct102), microdilution test (vitek 2 with aes) and double disk synergy test (ddst), as well as the microdilution test and molecular test.201323735062
inhibition of bacterial motility and spreading via release of cranberry derived materials from silicone substrates.the motility of bacteria plays a key role in their colonization of surfaces during infection. derivatives of cranberry fruit have been shown to interfere with bacterial motility. herein, we report on the incorporation of cranberry derived materials (cdms) into silicone substrates with the aim of impairing bacterial pathogen motility and spreading on the substrate surface. the release of cdms from the silicone substrates when soaking in an aqueous medium was quantified for a period of 24h. next, ...201323732805
occurrence of the ctx-m, shv and the tem genes among the extended spectrum β-lactamase producing isolates of enterobacteriaceae in a tertiary care hospital of north india.the present study was carried out to assess the prevalence of the extended spectrum beta lactamases (esbls) and to characterize the esbl types which were prevalent in our hospital.201323730637
the joint in vitro action of polymyxin b and miconazole against pathogens associated with canine otitis externa from three european countries.canine otitis externa, an inflammation of the external ear canal, can be maintained and worsened by bacterial or fungal infections. for topical treatment, combinations of anti-inflammatory and antimicrobial ingredients are mainly used.201323721182
linezolid desensitization for a patient with multiple medication hypersensitivity reactions.to describe a case in which a linezolid desensitization protocol was successfully used for a polymicrobial surgical wound infection in a patient with multiple drug hypersensitivity reactions.201523715068
plant-based hydrocarbon esters from tragia involucrata possess antimicrobial and anti-inflammatory activities.antimicrobial and anti-inflammatory activities of hydrocarbon esters obtained from tragia involucrata were evaluated by disk-diffusion (250 µg/ml), and broth-dilution (500-7.8 µg/ml), methods against bacteria. among the compounds, shellsol showed the most potent activity against burkholderia pseudomallei (khw), aeromonas hydrophila, staphylococcus aureus, bacillus subtilis, streptococcus pyogenes, klebsiella pneumoniae, proteus mirabilis, and streptococcus pneumoniae. interestingly, vinyl hexyle ...201323713670
outbreak of a cluster with epidemic behavior due to serratia marcescens after colistin administration in a hospital setting.serratia marcescens causes health care-associated infections with important morbidity and mortality. particularly, outbreaks produced by multidrug-resistant isolates of this species, which is already naturally resistant to several antibiotics, including colistin, are usually described with high rates of fatal outcomes throughout the world. thus, it is important to survey factors associated with increasing frequency and/or emergence of multidrug-resistant s. marcescens nosocomial infections. we r ...201323698525
first isolation of carbon dioxide-dependent proteus mirabilis from an uncomplicated cystitis patient with sjögren's syndrome.an uncomplicated cystitis caused by co2-dependent proteus mirabilis was observed in a 64-year-old japanese female patient with sjögren's syndrome in the aomori kyoritsu hospital, aomori, japan. the initial p. mirabilis isolate came from a midstream urine specimen containing large numbers of gram-negative, rod-shaped organisms that failed to grow on both drigalski agar and sheep blood agar incubated in ambient air. the organism did grow when the urine was cultured overnight on blood agar under an ...201323698488
regulation of the swarming inhibitor disa in proteus mirabilis.the disa gene encodes a putative amino acid decarboxylase that inhibits swarming in proteus mirabilis. 5' rapid amplification of cdna ends (race) and deletion analysis were used to identify the disa promoter. the use of a disa-lacz fusion indicated that flhd(4)c(2), the class i flagellar master regulator, did not have a role in disa regulation. the putative product of disa, phenethylamine, was able to inhibit disa expression, indicating that a negative regulatory feedback loop was present. trans ...201323687266
antimicrobial activity of prulifloxacin in comparison with other fluoroquinolones against community-acquired urinary and respiratory pathogens isolated in greece.prulifloxacin, the prodrug of ulifloxacin, is a broad-spectrum fluoroquinolone rather recently introduced in certain european countries. we compared the antimicrobial potency of ulifloxacin with that of other fluoroquinolones against common urinary and respiratory bacterial pathogens. the microbial isolates were prospectively collected between january 2007 and may 2008 from patients with community-acquired infections in greece. minimum inhibitory concentrations (mics) were determined for ciprofl ...201323686506
antimicrobial activity of schinus lentiscifolius (anacardiaceae).schinus lentiscifolius marchand (syn. schinus weinmannifolius engl) is a plant native to rio grande do sul (southern brazil) and has been used in brazilian traditional medicine as antiseptic and antimicrobial for the treatment of many different health problems as well as to treat leucorrhea and to assist in ulcer and wound healing. although it is a plant widely used by the population, there are no studies proving this popular use.201323684720
etiology and antimicrobial susceptibility pattern of bacterial pathogens from urinary tract infection.urinary tract infection (uti) is the most common infection in both community and hospital patients. in majority of the cases, empirical antimicrobial treatment is practiced before the laboratory results of urine culture. thus, antibiotic resistance may increase in urinary bacterial pathogens due to improper use of drugs. this study was designed to find out the etiological agents of uti and their prevalence, and to determine the antimicrobial susceptibility pattern of the bacterial pathogens isol ...201223671963
bacteria species identification by the statistical analysis of bacterial colonies fresnel patterns.it was demonstrated that statistical analysis of bacteria colonies fresnel patterns recorded in the optical system with converging spherical wave illumination is suitable for highly effective bacteria species classification. the proposed method includes fresnel patterns recording followed by image processing and the statistical analysis based on feature extraction, feature selection, classification and classification performance methods. examination performed on various bacteria species (salmone ...201323669989
surveillance of antibiotic susceptibility of enterobacteriaceae isolated from urine samples collected from community patients in a large metropolitan area, 2010-2012.antibiotic susceptibilities of large cohorts of enterobacteriaceae isolated from urine collected in the community are scarce. we report the susceptibilities of enterobacteriaceae isolated from urine of non-selected community populations in a metropolitan area (leeds and bradford, uk) over 2 years. isolates (n = 6614) were identified as follows: escherichia coli (n = 5436), klebsiella spp. (n = 525), proteus mirabilis (n = 305), and 15 other species (n = 290); 58 isolates were unidentified. ampic ...201423657157
dieselzymes: development of a stable and methanol tolerant lipase for biodiesel production by directed evolution.biodiesels are methyl esters of fatty acids that are usually produced by base catalyzed transesterification of triacylglyerol with methanol. some lipase enzymes are effective catalysts for biodiesel synthesis and have many potential advantages over traditional base or acid catalyzed transesterification. natural lipases are often rapidly inactivated by the high methanol concentrations used for biodiesel synthesis, however, limiting their practical use. the lipase from proteus mirabilis is a parti ...201323648063
effect of ph on the in vitro susceptibility of planktonic and biofilm-grown proteus mirabilis to the quinolone antimicrobials.to examine the effect of elevated ph, as reported during urinary catheter infections, on quinolone activity against the urease-producing pathogen proteus mirabilis.201323647563
antibacterial activity of mangrove leaf extracts against human pathogens.the antibacterial activity of leaf extract of mangroves, namely, rhizophora mucronata, sonneratia alba and exoecaria agallocha from chorao island, goa was investigated against human bacterial pathogens staphylococcus aureus, streptococcus sp., salmonella typhi, proteus vulgaris and proteus mirabilis. as compared to aqueous, ethanol extract showed broad-spectrum activity. the multidrug-resistant (mdr) bacteria salmonella typhi was inhibited by the ethanol extract of s. alba leaf whereas the other ...201223626390
antimicrobial susceptibility of inpatient urinary tract isolates of gram-negative bacilli in the united states: results from the study for monitoring antimicrobial resistance trends (smart) program: 2009-2011.the study for monitoring antimicrobial resistance trends is an ongoing global surveillance program that has monitored the susceptibilities of gram-negative bacilli from inpatient urinary tract infections (utis) since late 2009.201323623624
surveillance of multidrug resistant uropathogenic bacteria in hospitalized patients in indian.to record surveillance, antibiotic resistance of uropathogens of hospitalized patients over a period of 18 months.201323620859
proteus mirabilis uroepithelial cell adhesin (uca) fimbria plays a role in the colonization of the urinary tract.urinary tract infections (utis) are among the most common bacterial infections in humans. proteus mirabilis is an opportunistic pathogen, capable of causing severe utis, with serious kidney damage that may even lead to death. several virulence factors are involved in the pathogenicity of this bacterium. among these, adherence to the uroepithelium mediated by fimbriae appears to be a significant bacterial attribute related to urovirulence. proteus mirabilis expresses several types of fimbriae tha ...201323620155
a map kinase pathway in caenorhabditis elegans is required for defense against infection by opportunistic proteus species.caenorhabditis elegans innate immunity requires a conserved mitogen activated protein kinase (mapk) pathway that regulates the basal and pathogen-induced expression of immune effectors. being in the group of opportunistic pathogens, proteus spp. cause large number of nosocomial infections. since, proteus spp. do not cause death in wild type c. elegans, to understand the role and contribution of map kinase pathway, the mutants (sek-1 and pmk-1) of this pathway were employed. physiological experim ...201623597661
distribution of extended-spectrum β-lactamases, ampc β-lactamases, and carbapenemases among enterobacteriaceae isolates causing intra-abdominal infections in the asia-pacific region: results of the study for monitoring antimicrobial resistance trends (smart).the increasing trend of β-lactam resistance among enterobacteriaceae is a worldwide threat. enterobacteriaceae isolates causing intra-abdominal infections (iai) from the study for monitoring antimicrobial resistance trends (smart) collected in 2008 and 2009 from the asia-pacific region were investigated. detection of extended-spectrum β-lactamases (esbls), ampc β-lactamases, and carbapenemases was performed by multiplex pcr. a total of 699 enterobacteriaceae isolates with positive genotypic resu ...201323587958
emergence of salmonella genomic island 1 (sgi1) among proteus mirabilis clinical isolates in dijon, france.salmonella genomic island 1 (sgi1) is often encountered in antibiotic-resistant salmonella enterica and exceptionally in proteus mirabilis. we investigated the prevalence of sgi1-producing clinical isolates of p. mirabilis in our hospital (dijon, france).201323580563
putrescine importer plap contributes to swarming motility and urothelial cell invasion in proteus mirabilis.previously, we reported that the spea gene, encoding arginine decarboxylase, is required for swarming in the urinary tract pathogen proteus mirabilis. in addition, this previous study suggested that putrescine may act as a cell-to-cell signaling molecule (sturgill, g., and rather, p. n. (2004) mol. microbiol. 51, 437-446). in this new study, plap, a putative putrescine importer, was characterized in p. mirabilis. in a wild-type background, a plap null mutation resulted in a modest swarming defec ...201323572531
in silico maturation of binding-specificity of dna aptamers against proteus mirabilis.proteus mirabilis is a prominent cause of catheter-associated urinary tract infections (cautis) among patients undergoing long-term bladder catheterization. there are currently no effective means of preventing p. mirabilis infections, and strategies for prophylaxis and rapid early diagnosis are urgently required. aptamers offer significant potential for development of countermeasures against p. mirabilis cauti and are an ideal class of molecules for the development of diagnostics and therapeutic ...201323568752
the effects of bee pollen extracts on the broiler chicken's gastrointestinal microflora.the aim of this study was to investigate the effects of bee pollen ethanolic extracts on the in vivo gastrointestinal tract microflora colonization of broiler chickens. a completely randomized experiment based on six treatments (different concentrations of bee pollen - 0, 5, 15, 25, 35 and 45 g kg(-1) diet) was used during 7 weeks. the highest count of faecal enterococci was found in the experimental group with the addition of 15 g of pollen (8.85 ± 0.87 log cfu g(-1)) per 1 kg of feed mixture. ...201323548478
neonatal meningoventriculitis due to proteus mirabilis - a case report.a five day old full term born baby was admitted to our neonatal intensive care unit with seizures, opisthotonous posture and was icteric upto thigh. baby had a three day history of poor feeding, lethargy and abnormal body movements. mother was a 29 years old primigravida and had a normal vaginal delivery at home. sepsis profile of the patient was requested, lumbar puncture and ventricular tap was performed. patient was put on third generation cephalosporins, aminoglycosides and phenobarbitone. c ...201223543669
epidemiology and susceptibility of gram-negative appendicitis pathogens: smart 2008-2010.the study for monitoring antimicrobial resistance trends (smart) has tracked the in vitro activity of ertapenem and comparators against aerobic gram-negative bacteria from intra-abdominal infections since 2002. this report describes the epidemiology and susceptibility for clinical isolates associated with appendicitis, collected from 2008 to 2010.201323540793
community spread of extended-spectrum β-lactamase-producing escherichia coli, klebsiella pneumoniae and proteus mirabilis: a long-term study in japan.community-acquired infections caused by extended-spectrum β-lactamase (esbl)-producing bacteria, particularly ctx-m-producing escherichia coli, are a rising concern worldwide. there are few data from japan on the acquisition of esbls in the community or the influx of these bacteria into hospitals. therefore, we examined the prevalence of esbl carriage in outpatients, in order to estimate the spread of esbls in community settings. we analysed bacterial isolates from outpatient samples at our inst ...201323538565
postoperative infectious complications in our early experience with holmium laser enucleation of the prostate for benign prostatic hyperplasia.the objective of this study was to retrospectively investigate postoperative infectious complications (pics) in our early experience with holmium laser enucleation of the prostate (holep) followed by mechanical morcellation for symptomatic benign prostatic hyperplasia.201323526729
[comparative study of antibiotic resistance in bacteria isolated from burned patients during two periods (2005-2008, 2008-2011) and in two hospitals (hospital aziza othmana, trauma and burn center)].continuous monitoring of the bacterial flora and antibiotic resistance of the main bacteria involved in nosocomial infections helps improve treatment and prevention strategies.201323526277
outcomes in uk patients with hospital-acquired bacteraemia and the risk of catheter-associated urinary tract infections.there is lack of contemporary outcome data on patients with hospital-acquired infections that cause bacteraemia. we determined the risk factors for 7-day mortality and investigated the hypothesis that, compared with central venous catheter (cvc)-associated bacteraemic infections, catheter-associated bacteraemic urinary tract infections (utis) were significantly associated with 7-day mortality.201323520064
carbapenem susceptibilities and non-susceptibility concordance to different carbapenems amongst clinically important gram-negative bacteria isolated from intensive care units in taiwan: results from the surveillance of multicentre antimicrobial resistance in taiwan (smart) in 2009.to investigate the in vitro susceptibilities to various carbapenems amongst clinical gram-negative bacteria isolated from patients in intensive care units of ten major teaching hospitals in taiwan in 2009, a survey was conducted to determine the minimum inhibitory concentrations (mics) of ertapenem, imipenem, meropenem and doripenem against isolates of enterobacteriaceae (n = 594), pseudomonas aeruginosa (n = 185), acinetobacter baumannii (n = 192) and burkholderia cepacia (n = 23) using the aga ...201323507415
f2:a-:b- plasmid carrying the extended-spectrum β-lactamase bla(ctx-m-55/57) gene in proteus mirabilis isolated from a primate. 201323507413
serum in vivo and in vitro activity of single dose of ertapenem in surgical obese patients for prevention of ssis.despite progress made in the control of post-operative infections, the incidence of surgical site infections (ssis) is still high. an improper perioperative antibiotic use can expose patients to the risk of resistant microorganisms, and, in surgical obese patients, the drug dosage and infusion time are critical points. the aim of our study was to evaluate the effectiveness of ertapenem in the prophylaxis of ssis in obese patients undergoing general or bariatric surgery.201323504617
anti-biofilm effects of honey against wound pathogens proteus mirabilis and enterobacter cloacae.biofilm growth and its persistence within wounds have recently been suggested as contributing factors to impaired healing. the goal of this study was to investigate the anti-biofilm effects of several honey samples of different botanical origin, including manuka honey against proteus mirabilis and enterobacter cloacae wound isolates. quantification of biofilm formation was carried out using a microtiter plate assay. all honeys at a sub-inhibitory concentration of 10% (w/v) significantly reduced ...201423494861
chemical composition and antibacterial and cytotoxic activities of allium hirtifolium boiss.allium hirtifolium boiss. known as persian shallot, is a spice used as a traditional medicine in iran and, mediterranean region. in this study, the chemical composition of the hydromethanolic extract of this plant was analyzed using gc/ms. the result showed that 9-hexadecenoic acid, 11,14-eicosadienoic acid, and n-hexadecanoic acid are the main constituents. the antibacterial activity of the shallot extract was also examined by disk diffusion and microdilution broth assays. it was demonstrated t ...201223484141
tem-187, a new extended-spectrum β-lactamase with weak activity in a proteus mirabilis clinical strain.a proteus mirabilis clinical strain (7001324) was isolated from urine sample of a patient hospitalized in a long-term-care facility. pcr and cloning experiments performed with this strain identified a novel tem-type β-lactamase (tem-187) differing by four amino acid substitutions (leu21phe, arg164his, ala184val, and thr265met) from tem-1. this characterization provides further evidence for the diversity of extended-spectrum β-lactamases (esbl) produced by p. mirabilis and for their potential spr ...201323478954
aetiology and antibiotic resistance patterns of urinary tract infections in the elderly: a 6-month study.urinary tract infections (utis) are a common cause of bacteraemia in the elderly and are associated with a high probability of hospitalization. despite the impact of utis on health status and quality of life, a limited number of studies have evaluated their aetiology in this population. this study aimed to evaluate the microbial aetiology and pattern of susceptibility of bacteria causing utis in the elderly. for this purpose, a retrospective cohort study of elderly residents (n = 472, aged >65 y ...201323475904
effects of different substituents on the crystal structures and antimicrobial activities of six ag(i) quinoline compounds.the syntheses and single crystal x-ray structures of [ag(5-nitroquinoline)2]no3 (1), [ag(8-nitroquinoline)2]no3·h2o (2), [ag(6-methoxy-8-nitroquinoline)(no3)]n (3), [ag(3-quinolinecarbonitrile)(no3)]n (4), [ag(3-quinolinecarbonitrile)2]no3 (5), and [ag(6-quinolinecarboxylic acid)2]no3 (6) are described. as an alternative to solution chemistry, solid-state grinding could be used to prepare compounds 1 and 3, but the preparation of 4 and 5 in this way failed. the ag(i) ions in the monomeric compou ...201323458224
screening of solvent dependent antibacterial activity of prunus domestica.fruit of prunus domestica was extracted in ethanol. the ethanol extract was further extracted with two solvents ethyl acetate and chloroform. the crude ethanol extract and two fractions (ethyl acetate and chloroform) were screened for their antibacterial activity using the agar well diffusion method .they were tested against nine bacteria; five gram positive bacteria (staphylococcus aureus, streptococcuc intermedius, bacillus cereus, bacillus pumilus) and four gram negative bacteria (eschrichia ...201323455215
urinary tract infection among symptomatic outpatients visiting a tertiary hospital based in midwestern nigeria.microbial pathogens implicated in urinary tract infection and their antibiotic susceptibility patterns as prevalent in uti symptomatic outpatients resident in benin city, nigeria was the focus of this study. one hundred (100) midstream urine samples were collected into sterile plastic universal bottles from outpatients who visited the university of benin teaching hospital, nigeria and who were tentatively diagnosed as manifesting symptoms of uti. patients were referred to the medical microbiolog ...201323445708
eugenol alters the integrity of cell membrane and acts against the nosocomial pathogen proteus mirabilis.eugenol, a member of the phenylpropanoids class of chemical compounds, is a clear to pale yellow oily liquid extracted from certain essential oils especially from clove oil, nutmeg, cinnamon, and bay leaf. the antibacterial activity of eugenol and its mechanism of bactericidal action against proteus mirabilis were evaluated. treatment with eugenol at their minimum inhibitory concentration [0.125 % (v/v)] and minimum bactericidal concentration [0.25 % (v/v)] reduced the viability and resulted in ...201323444040
infectious risk assessment of unsafe handling practices and management of clinical solid waste.the present study was undertaken to determine the bacterial agents present in various clinical solid wastes, general waste and clinical sharp waste. the waste was collected from different wards/units in a healthcare facility in penang island, malaysia. the presence of bacterial agents in clinical and general waste was determined using the conventional bacteria identification methods. several pathogenic bacteria including opportunistic bacterial agent such as pseudomonas aeruginosa, salmonella sp ...201323435587
bacterial isolates from infected wounds and their antibiotic susceptibility pattern: some remarks about wound infection.wound infection plays an important role in the development of chronicity, delaying wound healing. this study aimed to identify the bacterial pathogens present in infected wounds and characterise their resistance profile to the most common antibiotics used in therapy. three hundred and twelve wound swab samples were collected from 213 patients and analysed for the identification of microorganisms and for the determination of their antibiotic susceptibility. patients with diverse type of wounds we ...201523433007
chemical composition and antimicrobial and allelopathic activity of tunisian conyza sumatrensis (retz.) e.walker essential oils.conyza sumatrensis (retz.) e.walker (asteraceae) is a spontaneous annual herb, fairly widespread throughout tunisia, which has rarely been studied or valued in any sector. essential oils were obtained by hydrodistillation of different parts (flower heads, leaves, stems, and roots) of c. sumatrensis plants, which were collected in autumn (november 2007) at the flowering stage in the area of monastir, tunisia. in total, 98 compounds, representing 88.1-99.3% of the oil composition, were identified ...201323418168
effects of taishan pinus massoniana pollen polysaccharide on the subunit vaccine of proteus mirabilis in birds.three adjuvants, namely, taishan pinus massoniana pollen polysaccharide (tppps), white mineral oil (wo) and propolis (pp), were added to the outer membrane protein (omp) of proteus mirabilis (p. mirabilis) and their effects were compared. three hundred 1-day-old chicks were randomly divided into five groups (i-v), with 60 chicks per group, and injected subcutaneously with wo-omp vaccine (i), pp-omp vaccine (ii), tppps-omp vaccine (iii), omp-only vaccine (iv) and physiological saline (v) at 3, 7 ...201323403027
characteristics of bacteremia caused by extended-spectrum beta-lactamase-producing proteus mirabilis.although proteus mirabilis is a common human pathogen, bacteremia caused by the organism, especially strains producing extended-spectrum beta-lactamase (esbl), has rarely been investigated. we examined 64 cases of p. mirabilis bacteremia identified in the minami ibaraki area, japan, between 2001 and 2010 and compared the characteristics of cases with esbl-producing and esbl-non-producing strains (13 and 51 cases, respectively). all esbl-producing strains with the gene encoding the ctx-m-2-group ...201323371453
antibiotic resistance patterns of outpatient pediatric urinary tract infections.we characterize the current national patterns of antibiotic resistance of outpatient pediatric urinary tract infection.201323369720
studies on water quality and pathogenic bacteria in coastal water langkawi, malaysia.a study on physico-chemical parameters and pathogenic bacterial community was carried out at the coastal waters of pulau tuba island, langkawi. the physico-chemical parameters such as temperature (27.43-28.88 degrees c), dissolved oxygen (3.79-6.49 mg l(-1)), ph (7.72-8.20), salinity (33.10-33.96 ppt), total dissolved solids (32.27-32.77 g l(-1)) and specific conductivity (49.83-51.63 ms cm(-1)) were observed. station 3 and station 4 showed highest amount of nitrates (26.93 and 14.61 microg at n ...201223360015
flgm proteins from different bacteria exhibit different structural characteristics.intrinsically disordered proteins (idps) are a unique class of proteins that do not require a stable structure for function. the importance of idps in many biological processes has been established but there remain unanswered questions about their evolution and conservation of their disordered state within a protein family. our group has been studying the structural similarities among orthologous flgm proteins, a model class of idps. we have previously shown that the flgm protein from the thermo ...201323352839
analysis of the spectrum and antibiotic resistance of uropathogens in vitro: results based on a retrospective study from a tertiary hospital.antibiotic resistance of uropathogens in urinary tract infections (utis) is increasing worldwide. this study aimed to compare the spectrum and antimicrobial resistance of uropathogens in community-acquired utis (cautis) and nosocomial-acquired utis (nautis) at a tertiary hospital in china.201323352074
characterization of multidrug-resistant proteus mirabilis isolated from chicken carcasses.this study investigated genotypic and phenotypic features of antimicrobial resistance of proteus mirabilis isolated from chicken products. resistance to a broad spectrum of antimicrobial agents was commonly observed in the test isolates: tetracycline (100%), sulfamethoxazole (80%), chloramphenicol (66%), nalidixic acid (66%), ampicillin (60%), streptomycin (56%), ciprofloxacin (52%), kanamycin (46%), gentamicin (38%), ceftriaxone (36%), cefotaxime (34%), ceftiofur (22%), and amoxicillin-clavulan ...201323351030
one-step production of α-ketoglutaric acid from glutamic acid with an engineered l-amino acid deaminase from proteus mirabilis.currently, α-ketoglutaric acid (α-kg) is industrially produced by multi-step chemical synthesis, which can cause heavy environmental pollution. here we reported a simple one-step approach for the production of α-kg by transforming l-glutamic acid with an engineered l-amino acid deaminase (l-aad) from proteus mirabilis. first, to facilitate the purification of membrane-bound l-aad, one n-terminal transmembrane region (from 21 to 87th nucleotide) was removed from l-aad to block the binding of l-aa ...201323333917
microbial uropathogens and their antibiotic resistance profile from hospitalized patients in central alabama.urinary tract infections remain a common problem in inpatient care. they are highly challenging to provide effective initial therapy without sensitivity data. the purpose of this study was to survey the uropathogens and their sensitivity profile at a hospital in central alabama and to guide experiential antibiotic selection. this was the first reported study on bacterial uropathogens and their antibiotic resistance profile at this central alabama hospital. the survey period was between july 2009 ...201223330509
the novel ctx-m-116 β-lactamase gene discovered in proteus mirabilis is composed of parts of the ctx-m-22 and ctx-m-23 genes.the novel β-lactamase gene bla(ctx-m-116) was identified in a proteus mirabilis nosocomial isolate recovered from the urine of a patient in moscow in 2005. dna sequence analysis showed bla(ctx-m-116) to be a hybrid gene consisting of 5' bla(ctx-m-23) (nucleotides 1 to 278) and 3' bla(ctx-m-22) (nucleotides 286 to 876) moieties separated by an intervening putative site of recombination (gttaaat). a retrospective analysis of available bla(ctx-m) genes in the genbank database revealed 19 bla(ctx-m) ...201323318795
initiation of swarming motility by proteus mirabilis occurs in response to specific cues present in urine and requires excess l-glutamine.proteus mirabilis, a leading cause of catheter-associated urinary tract infection (cauti), differentiates into swarm cells that migrate across catheter surfaces and medium solidified with 1.5% agar. while many genes and nutrient requirements involved in the swarming process have been identified, few studies have addressed the signals that promote initiation of swarming following initial contact with a surface. in this study, we show that p. mirabilis cauti isolates initiate swarming in response ...201323316040
crystal structure of proteus mirabilis lipase, a novel lipase from the proteus/psychrophilic subfamily of lipase family i.1.bacterial lipases from family i.1 and i.2 catalyze the hydrolysis of triacylglycerol between 25-45°c and are used extensively as biocatalysts. the lipase from proteus mirabilis belongs to the proteus/psychrophilic subfamily of lipase family i.1 and is a promising catalyst for biodiesel production because it can tolerate high amounts of water in the reaction. here we present the crystal structure of the proteus mirabilis lipase, a member of the proteus/psychrophilic subfamily of i.1lipases. the s ...201223300806
comparison of antibiotic resistance patterns in collections of escherichia coli and proteus mirabilis uropathogenic strains.escherichia coli and proteus mirabilis are important urinary tract pathogens. the constant increase in the antibiotic resistance of clinical bacterial strains has become an important clinical problem. the aim of this study was to compare the antibiotic resistance of 141 clinical (sweden and poland) and 42 laboratory (czech republic) p. mirabilis strains and 129 clinical (poland) uropathogenic e. coli strains. the proportion of unique versus diverse patterns in swedish clinical and laboratory p. ...201323283741
qualitative analysis of preputial and vaginal bacterial microbiota in owl monkeys (aotus azarai infulatus) raised in captivity.the aim of this study was to identify the aerobic bacteria of the preputial and vaginal microbiota in owl monkeys that have been raised in captivity and to evaluate the antimicrobial susceptibility of these bacteria by gender and social organization.201223278502
molecular diversity of class 2 integrons in antibiotic-resistant gram-negative bacteria found in wastewater environments in china.the molecular architecture of class 2 integrons among gram-negative bacteria from wastewater environments was investigated in jinan, china. out of the 391 antibiotic-resistant bacteria found, 38 isolates harboring class 2 integrons encoding potentially transferrable genes that could confer antibiotic resistance were found. these isolates were classified into 19 rep-pcr types. these strains were identified using 16s rrna gene sequencing and found to be as follows: proteus mirabilis (16), escheric ...201223264021
chemical and antimicrobial studies on the essential oil from salvia santolinifolia boiss.in view of the reputation of genus salvia in folklore medicine and its abundance in our region, the chemical composition and antimicrobial activity of essential oil from s. santolinifolia boiss. was analyzed. chemical analysis, using gas chromatography and gas chromatography mass spectrometry, retention indices and c-13 nuclear magnetic resonance spectroscopy has resulted in identification of 116 constituents, comprising about 97% of the total constituents. out of these 116, 78 constituents are ...201323261726
infection-responsive drug delivery from urinary biomaterials controlled by a novel kinetic and thermodynamic approach.the ph-dependent physicochemical properties of the antimicrobial quinolone, nalidixic acid, were exploited to achieve 'intelligent' drug release from a potential urinary catheter coating, poly(2-hydroxyethylmethacrylate) (p(hema)), in direct response to the elevated ph which occurs at the onset of catheter infection.201323229857
activity of proteus mirabilis flil is viscosity dependent and requires extragenic dna.proteus mirabilis is a urinary tract pathogen and well known for its ability to move over agar surfaces by flagellum-dependent swarming motility. when p. mirabilis encounters a highly viscous environment, e.g., an agar surface, it differentiates from short rods with few flagella to elongated, highly flagellated cells that lack septa and contain multiple nucleoids. the bacteria detect a surface by monitoring the rotation of their flagellar motors. this process involves an enigmatic flagellar prot ...201323222728
persistence of bacteria like pseudomonas aeruginosa in non-healing venous ulcers.there is evidence that chronic inflammation due to bacterial wound colonization delays wound healing.201223220150
a binational cohort study of intestinal colonization with extended-spectrum β-lactamase-producing proteus mirabilis in patients admitted to rehabilitation centres.the aims of our study were to analyse the risk factors for colonization by extended-spectrum β-lactamases (esbl)-producing proteus mirabilis (esbl-pm) in rehabilitation patients and to characterize the molecular features of these strains. the study was conducted in two rehabilitation centres located in rome, italy (fondazione santa lucia irccs (fsl)), and tel-aviv, israel (tel-aviv sourasky medical center (tasmc)). carriage of esbl-pm was surveyed by rectal swabs. strain typing was performed by ...201323210906
antibiotic utilization pattern in a general medical ward of a tertiary medical center in saudi arabia.to assess the pattern of antibiotic utilization and outcome of patients with bacteremia in a general medical ward, all positive blood cultures (bc) over a 12-month period from january 1994 to december 1995 were retrospectively reviewed. fifty-two positive bc were recorded in 43 patients (63% males, 37% females; mean age 65.9 ± 16.6). 72% of the patients received antibiotics before or soon after obtaining the bc, and ceftriaxone was the most frequently-prescribed antibiotic (41.93%), either alone ...201123210003
characterization of aminoglycoside-modifying enzymes in enterobacteriaceae clinical strains and characterization of the plasmids implicated in their diffusion.a total of 788 clinical enterobacteriaceae were collected to describe the aminoglycoside-modifying genes (ame genes) and to characterize the plasmids that carry these genes. among the 788 strains collected, 330 (41.8%) were aminoglycoside-resistant: 264 escherichia coli (80%), 33 proteus mirabilis (10%), 10 klebsiella pneumoniae (3%), six k. oxytoca (1.8%), five enterobacter cloacae (1.5%), three morganella morganii (0.9%), three providencia stuartii (0.9%), two salmonella enterica (0.6%), and o ...201323206280
[purple urine bag syndrome in two institutionalised patients].purple urine bag syndrome (pubs) is an uncommon but particularly striking phenomenon characterised by a chemical reaction involving the urine, plastic and certain enzymes from some sulphatase- and phosphatase-producing bacteria, including proteus mirabilis, escherichia coli and morganella morganii, amongst others. following this reaction, the catheter and the bag may be stained red, blue or purple. this phenomenon tends to occur in patients with multiple pathology and with urinary catheters, as ...201323199817
antimicrobial lectin from schinus terebinthifolius leaf.schinus terebinthifolius leaves are used for treating human diseases caused by micro-organisms. this work reports the isolation, characterization and antimicrobial activity of s. terebinthifolius leaf lectin (stell).201323190078
prevalence and risk factors of asymptomatic bacteriuria among pregnant nigerians infected with hiv.there are conflicting report on the association of hiv infection and asymptomatic bacteriuria (asb). most of these studies were from areas with low hiv burden. this study determined the prevalence and risk factors of asb in hiv positive pregnant women.201323186370
contribution of structural domains to the activity of ribonuclease 7 against uropathogenic bacteria.ribonuclease 7 (rnase 7) is a 14.5-kda peptide that possesses potent antimicrobial properties against gram-negative and gram-positive bacteria and is expressed in a variety of epithelial tissues. little is known about its mechanisms of action and the determinants of its antimicrobial properties. the objective of this study was to identify the intrinsic functional domains of rnase 7 that influence its activity against uropathogenic bacteria. a series of rnase 7 fragments were generated that conta ...201323183439
comparison of phenotypic and virulence genes characteristics in human and chicken isolates of proteus mirabilis.the objective of this work is to compare the phenotypic and virulence genes characteristics in human and chicken isolates of proteus mirabilis. the bacterial examination of 50 livers of individual broilers, marketed by four major outlets, revealed a high recovery of p. mirabilis (66%), and a low recovery frequency of salmonella spp. (4%), serratia odorifera (2%), citrobacter brakii (2%), and providencia stuartii (2%). the phenotypic biochemical characterization of the recovered 33 chicken isolat ...201223182140
flagellum density regulates proteus mirabilis swarmer cell motility in viscous environments.proteus mirabilis is an opportunistic pathogen that is frequently associated with urinary tract infections. in the lab, p. mirabilis cells become long and multinucleate and increase their number of flagella as they colonize agar surfaces during swarming. swarming has been implicated in pathogenesis; however, it is unclear how energetically costly changes in p. mirabilis cell morphology translate into an advantage for adapting to environmental changes. we investigated two morphological changes th ...201323144253
the effect of copper(ii), iron(ii) sulphate, and vitamin c combinations on the weak antimicrobial activity of (+)-catechin against staphylococcus aureus and other microbes.few attempts have been made to improve the activity of plant compounds with low antimicrobial efficacy. (+)-catechin, a weak antimicrobial tea flavanol, was combined with putative adjuncts and tested against different species of bacteria. copper(ii) sulphate enhanced (+)-catechin activity against pseudomonas aeruginosa but not staphylococcus aureus, proteus mirabilis or escherichia coli. attempts to raise the activity of (+)-catechin against two unresponsive species, s. aureus and e. coli, with ...201223138340
molecular structure, normal coordinate analysis, harmonic vibrational frequencies, natural bond orbital, td-dft calculations and biological activity analysis of antioxidant drug 7-hydroxycoumarin.in this work, we report harmonic vibrational frequencies, molecular structure, nbo and homo, lumo analysis of umbelliferone also known as 7-hydroxycoumarin (7hc). the optimized geometric bond lengths and bond angles obtained by computation (monomer and dimmer) shows good agreement with experimental xrd data. harmonic frequencies of 7hc were determined and analyzed by dft utilizing 6-311+g(d,p) as basis set. the assignments of the vibrational spectra have been carried out with the help of normal ...201323123244
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