Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| clinical and corneal microbial profile of infectious keratitis in a high hiv prevalence setting in rural south africa. | the purpose of this investigation was to determine the clinical and corneal microbial profile of infectious keratitis in a high human immunodeficiency virus (hiv) prevalence setting in rural south africa. data in this cross-sectional study were collected from patients presenting with symptoms of infectious keratitis (n = 46) at the ophthalmology outpatient department of three hospitals in rural south africa. corneal swabs were tested for herpes simplex virus type 1 (hsv-1) and 2 (hsv-2), varicel ... | 2016 | 27236644 |
| clinical impact of maldi-tof ms identification and rapid susceptibility testing on adequate antimicrobial treatment in sepsis with positive blood cultures. | shortening the turn-around time (tat) of positive blood culture (bc) identification (id) and susceptibility results is essential to optimize antimicrobial treatment in patients with sepsis. we aimed to evaluate the impact on antimicrobial prescription of a modified workflow of positive bcs providing id and partial susceptibility results for enterobacteriaceae (eb), pseudomonas aeruginosa and staphylococcus aureus on the day of bc positivity detection. this study was divided into a pre-interventi ... | 2016 | 27228001 |
| extended spectrum beta-lactamase producing enterobacteriaceae causing bloodstream infections in rural ghana, 2007-2012. | high prevalence of extended spectrum beta-lactamase (esbl) producing enterobacteriaceae threatens treatment options for invasive bloodstream infections in sub-saharan africa. | 2016 | 27222489 |
| length of preoperative hospital stay: a risk factor for reducing surgical infection in femoral fracture cases. | to analyze infections of the surgical site among patients undergoing clean-wound surgery for correction of femoral fractures. | 2015 | 27218074 |
| high intravascular tissue factor-but not extracellular microvesicles-in septic patients is associated with a high saps ii score. | sepsis is associated with coagulation abnormalities, and a high content of intravascular tissue factor (tf) may contribute to the development of multisystem organ failure. circulating microvesicles (mvs) are increased during sepsis and characterized by their phosphatidylserine content. it is unclear whether mvs-as a part of the host response to the infection-are beneficial or rather contribute to systemic complications in sepsis. in the present prospective clinical pilot study, we investigated w ... | 2016 | 27217958 |
| spread of plasmids carrying multiple ges variants. | five ges-producing enterobacteriaceae isolates that displayed an extended-spectrum β-lactamase (esbl) phenotype harbored two ges variants: ges-7 esbl and ges-6 carbapenemase. in all isolates, the two ges alleles were located on the same integron that was inserted into an 80-kb incm1 self-conjugative plasmid. whole-genome sequencing suggested in vivo horizontal gene transfer of the plasmid along with clonal diffusion of enterobacter cloacae to our knowledge, this is the first description in europ ... | 2016 | 27216071 |
| detection of the mcr-1 colistin resistance gene in carbapenem-resistant enterobacteriaceae from different hospitals in china. | the spread of the plasmid-mediated colistin resistance gene, mcr-1, into carbapenem-resistant enterobacteriaceae (cre) clinical isolates poses a significant threat to global health. here we report the identification of three mcr-1-harboring carbapenem-resistant escherichia coli strains, collected from three patients in two provinces in china. our results show that mcr-1-harboring cre strains have started to spread in different hospitals in china. in addition, this report presents the first descr ... | 2016 | 27216058 |
| ultrasound guided placement of the distal catheter in paediatric ventriculoatrial shunts-an appraisal of efficacy and complications. | ventriculoatrial (va) shunts are commonly used as a second-line treatment of hydrocephalus when the peritoneum is an unsuitable site for the distal catheter. many centres now utilise ultrasound and interventional radiology techniques to aid placement of the distal catheter. the purpose of this study was to conduct a contemporary audit of va shunting in children using interventional radiology techniques for placement of the distal catheter. | 2016 | 27207611 |
| a novel multi-epitope recombined protein for diagnosis of human brucellosis. | in epidemic regions of the world, brucellosis is a reemerging zoonosis with minimal mortality but is a serious public hygiene problem. currently, there are various methods for brucellosis diagnosis, however few of them are available to be used to diagnose, especially for serious cross-reaction with other bacteria. | 2016 | 27206475 |
| characterisation of candida within the mycobiome/microbiome of the lower respiratory tract of icu patients. | whether the presence of candida spp. in lower respiratory tract (lrt) secretions is a marker of underlying disease, intensive care unit (icu) treatment and antibiotic therapy or contributes to poor clinical outcome is unclear. we investigated healthy controls, patients with proposed risk factors for candida growth in lrt (antibiotic therapy, icu treatment with and without antibiotic therapy), icu patients with pneumonia and antibiotic therapy and candidemic patients (for comparison of truly inva ... | 2016 | 27206014 |
| bacterial cholangitis, cholecystitis, or both in dogs. | bacterial cholangitis and cholecystitis are rarely reported, poorly characterized diseases in the dog. | 2016 | 27203848 |
| antibacterial effect of copper on microorganisms isolated from bovine mastitis. | the antimicrobial properties of copper have been recognized for several years; applying these properties to the prevention of diseases such as bovine mastitis is a new area of research. the aim of the present study was to evaluate in vitro the antimicrobial activity of copper on bacteria isolated from subclinical and clinical mastitis milk samples from two regions in chile. a total of 327 microorganisms were recovered between march and september 2013, with different prevalence by sample origin ( ... | 2016 | 27199953 |
| biologically based methods for control of fumonisin-producing fusarium species and reduction of the fumonisins. | infection by the fumonisin-producing fusarium spp. and subsequent fumonisin contamination of maize adversely affect international trade and economy with deleterious effects on human and animal health. in developed countries high standards of the major food suppliers and retailers are upheld and regulatory controls deter the importation and local marketing of fumonisin-contaminated food products. in developing countries regulatory measures are either lacking or poorly enforced, due to food insecu ... | 2016 | 27199904 |
| [antibibiotic resistance by nosocomial infections' causal agents]. | the antibibiotic resistance by nosocomial infections (ni) causal agents constitutes a seriously global problematic that involves the mexican institute of social security's regional general hospital 1 in chihuahua, mexico; although with special features that required to be specified and evaluated, in order to concrete an effective therapy. | 2016 | 27197104 |
| correlating cleaning thoroughness with effectiveness and briefly intervening to affect cleaning outcomes: how clean is cleaned? | the most efficient approach to monitoring and improving cleaning outcomes remains unresolved. we sought to extend the findings of a previous study by determining whether cleaning thoroughness (dye removal) correlates with cleaning efficacy (absence of molecular or cultivable biomaterial) and whether one brief educational intervention improves cleaning outcomes. | 2016 | 27196635 |
| preliminary survey of local bacteriophages with lytic activity against multi-drug resistant bacteria. | bacteriophages (phages) represent a potential alternative for combating multi-drug resistant bacteria. because of their narrow host range and the ever emergence of novel pathogen variants the continued search for phages is a prerequisite for optimal treatment of bacterial infections. here we performed an ad hoc survey in the surroundings of a university hospital for the presence of phages with therapeutic potential. to this end, 16 aquatic samples of different origins and locations were tested s ... | 2016 | 27194637 |
| [distribution and drug sensitivity test of bacteria of patients on chronic rhinosinusitis with or without nasal polyps]. | to study the distribution and drug sensitivity test of bacteria of patients on chronic rhinosinusitis with or without nasal polyps. | 2016 | 27192905 |
| infectious disease risk associated with contaminated propofol anesthesia, 1989-2014(1). | administration of propofol, the most frequently used intravenous anesthetic worldwide, has been associated with several iatrogenic infections despite its relative safety. little is known regarding the global epidemiology of propofol-related outbreaks and the effectiveness of existing preventive strategies. in this overview of the evidence of propofol as a source of infection and appraisal of preventive strategies, we identified 58 studies through a literature search in pubmed, embase, and lilacs ... | 2016 | 27192163 |
| evaluation of heavy metal removal from wastewater in a modified packed bed biofilm reactor. | for the effective application of a modified packed bed biofilm reactor (pbbr) in wastewater industrial practice, it is essential to distinguish the tolerance of the system for heavy metals removal. the industrial contamination of wastewater from various sources (e.g. zn, cu, cd and ni) was studied to assess the impacts on a pbbr. this biological system was examined by evaluating the tolerance of different strengths of composite heavy metals at the optimum hydraulic retention time (hrt) of 2 hour ... | 2016 | 27186636 |
| a novel family viii carboxylesterase hydrolysing third- and fourth-generation cephalosporins. | a metagenomic library was constructed from a soil sample of spindle tree-rhizosphere. from this library, one clone with esterase activity was selected. the sequence analysis revealed an open reading frame (eststr1) encoded protein of 390 amino acids. eststr1 is a family viii carboxylesterase and retains the s-x-x-k motif conserved in both family viii carboxylesterases and class c β-lactamases. the eststr1 gene was overexpressed in e. coli and the recombinant protein was purified by purified by m ... | 2016 | 27186489 |
| isolation and characterization of escherichia coli sequence type 131 and other antimicrobial-resistant gram-negative bacilli from clinical stool samples from veterans. | emerging multidrug-resistant (mdr) gram-negative bacilli (gnb), including escherichia coli sequence type 131 (st131) and its resistance-associated h30 subclone, constitute an ever-growing public health threat. their reservoirs and transmission pathways are incompletely defined. to assess diarrheal stools as a potential reservoir for st131-h30 and other mdr gnb, we cultured 100 clinical stool samples from a veterans affairs medical center clinical laboratory (october to december 2011) for fluoroq ... | 2016 | 27185805 |
| in vitro emergence of high persistence upon periodic aminoglycoside challenge in the eskape pathogens. | health care-associated infections present a major threat to modern medical care. six worrisome nosocomial pathogens-enterococcus faecium, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, and enterobacter spp.-are collectively referred to as the "eskape bugs." they are notorious for extensive multidrug resistance, yet persistence, or the phenotypic tolerance displayed by a variant subpopulation, remains underappreciated in these pathogens. importantly ... | 2016 | 27185802 |
| use of attenuated but metabolically competent salmonella as a probiotic to prevent or treat salmonella infection. | salmonella enterica is among the most burdensome of foodborne disease agents. there are over 2,600 serovars that cause a range of disease manifestations ranging from enterocolitis to typhoid fever. while there are two vaccines in use in humans to protect against typhoid fever, there are none that prevent enterocolitis. if vaccines preventing enterocolitis were to be developed, they would likely protect against only one or a few serovars. in this report, we tested the hypothesis that probiotic or ... | 2016 | 27185789 |
| groel/es inhibitors as potential antibiotics. | we recently reported results from a high-throughput screening effort that identified 235 inhibitors of the escherichia coli groel/es chaperonin system [bioorg. med. chem. lett.2014, 24, 786]. as the groel/es chaperonin system is essential for growth under all conditions, we reasoned that targeting groel/es with small molecule inhibitors could be a viable antibacterial strategy. extending from our initial screen, we report here the antibacterial activities of 22 groel/es inhibitors against a pane ... | 2016 | 27184767 |
| membrane anchoring stabilizes and favors secretion of new delhi metallo-β-lactamase. | carbapenems, 'last-resort' β-lactam antibiotics, are inactivated by zinc-dependent metallo-β-lactamases (mbls). the host innate immune response withholds nutrient metal ions from microbial pathogens by releasing metal-chelating proteins such as calprotectin. we show that metal sequestration is detrimental for the accumulation of mbls in the bacterial periplasm, because those enzymes are readily degraded in their nonmetallated form. however, the new delhi metallo-β-lactamase (ndm-1) can persist u ... | 2016 | 27182662 |
| a single-center, six-year evaluation of the role of pulsed-field gel electrophoresis in suspected burn center outbreaks. | pulsed-field gel electrophoresis (pfge) has been used as an adjunct to traditional hospital epidemiology in numerous outbreak investigations, including in burn centers. however, its most effective real-world application remains unclear, with few longitudinal descriptions of use. | 2016 | 27180602 |
| draft genome sequence of hydrocarbon-degrading enterobacter cloacae strain s1:cnd1, isolated from crude oil-contaminated soil from the noonmati oil refinery, guwahati, assam, india. | we report here the 4.57-mb draft genome sequence of hydrocarbon-degrading enterobacter cloacae strain s1:cnd1 isolated from oil-contaminated soil in guwahati, india. s1:cnd1 contains 4,205 coding sequences and has a g+c content of 57.45%. this is the first report of the genome sequence of an e. cloacae adapted to an oil-contaminated environment. | 2016 | 27174279 |
| molecular characterization of class b carbapenemases in advanced stage of dissemination and emergence of class d carbapenemases in enterobacteriaceae from croatia. | carbapenemases involved in acquired carbapenem resistance in enterobacteriaceae belong to ambler class a serin β-lactamases, class b metallo-β-lactamases (mbl) or class d oxa-48-like β-lactamases. the aim of the present study was to analyse the molecular epidemiology and the mechanisms and routes of spread of class b and class d carbapenemases in croatia. in total 68 isolates were analyzed. antibiotic susceptibility was determined by broth microdilution method. pcr was used to detect antibiotic- ... | 2016 | 27174090 |
| epidemiology and resistance patterns of bacterial and fungal colonization of biliary plastic stents: a prospective cohort study. | plastic stents used for the treatment of biliary obstruction will become occluded over time due to microbial colonization and formation of biofilms. treatment of stent-associated cholangitis is often not effective because of inappropriate use of antimicrobial agents or antimicrobial resistance. we aimed to assess the current bacterial and fungal etiology of stent-associated biofilms, with particular emphasis on antimicrobial resistance. | 2016 | 27171497 |
| kinetic characterization of ges-22 β-lactamase harboring the m169l clinical mutation. | the class a β-lactamase ges-22 has been identified in acinetobacter baumannii isolates in turkey, and subsequently shown to differ from ges-11 by a single substitution (m169l). because m169 is part of the omega loop, a structure that is known to have major effects on substrate selectivity in class a β-lactamases, we expressed, purified and kinetically characterized this novel variant. our results show that compared with ges-11(6 × his), ges-22(6 × his) displays more efficient hydrolysis of penic ... | 2016 | 27168312 |
| insights into antimicrobial peptides from spiders and scorpions. | the venoms of spiders and scorpions contain a variety of chemical compounds. antimicrobial peptides (amps) from these organisms were first discovered in the 1990s. as of may 2015, there were 42 spider's and 63 scorpion's amps in the antimicrobial peptide database (http://aps.unmc.edu/ap). these peptides have demonstrated broad or narrow-spectrum activities against bacteria, fungi, viruses, and parasites. in addition, they can be toxic to cancer cells, insects and erythrocytes. to provide insight ... | 2016 | 27165405 |
| proteomic response of β-lactamases-producing enterobacter cloacae complex strain to cefotaxime-induced stress. | bacteria of the enterobacter cloacae complex are among the ten most common pathogens causing nosocomial infections in the usa. consequently, increased resistance to β-lactam antibiotics, particularly expanded-spectrum cephalosporins like cefotaxime (ctx), poses a serious threat. differential in-gel electrophoresis (dige), followed by lc-ms/ms analysis and bioinformatics tools, was employed to investigate the survival mechanisms of a multidrug-resistant e. hormaechei subsp. steigerwaltii 51 carry ... | 2016 | 27162211 |
| global dissemination of blakpc into bacterial species beyond klebsiella pneumoniae and in vitro susceptibility to ceftazidime-avibactam and aztreonam-avibactam. | the klebsiella pneumoniae carbapenemase (kpc), first described in the united states in 1996, is now a widespread global problem in several gram-negative species. a worldwide surveillance study collected gram-negative pathogens from 202 global sites in 40 countries during 2012 to 2014 and determined susceptibility to β-lactams and other class agents by broth microdilution testing. molecular mechanisms of β-lactam resistance among carbapenem-nonsusceptible enterobacteriaceae and pseudomonas aerugi ... | 2016 | 27161636 |
| mobilization of the salmonella genomic island sgi1 and the proteus genomic island pgi1 by the a/c2 plasmid carrying blatem-24 harboured by various clinical species of enterobacteriaceae. | the objective of this study was to transfer the salmonella genomic islands (gis) sgi1 and sgi1-v and the proteus gi pgi1-pmesc to clinical isolates of enterobacteriaceae harbouring an a/c2 plasmid. | 2016 | 27150396 |
| virulence characteristics of extraintestinal pathogenic escherichia coli deletion of gene encoding the outer membrane protein x. | outer membrane protein x (ompx) and its homologues have been proposed to contribute to the virulence in various bacterial species. but, their role in virulence of extraintestinal pathogenic escherichia coli (expec) is yet to be determined. this study evaluates the role of ompx in expec virulence in vitro and in vivo using a clinical strain ppecc42 of porcine origin. the ompx deletion mutant exhibited increased swimming motility and decreased adhesion to, and invasion of pulmonary epithelial a549 ... | 2016 | 27149893 |
| epidemiology and molecular characterization of multidrug-resistant gram-negative bacteria in southeast asia. | multidrug-resistant gram-negative bacteria (mdrgn), including extended-spectrum β-lactamases (esbls) and multidrug-resistant glucose-nonfermenting gram-negative bacilli (nonfermenters), have emerged and spread throughout southeast asia. | 2016 | 27148448 |
| therapeutic manuka honey: no longer so alternative. | medicinal honey research is undergoing a substantial renaissance. from a folklore remedy largely dismissed by mainstream medicine as "alternative", we now see increased interest by scientists, clinical practitioners and the general public in the therapeutic uses of honey. there are a number of drivers of this interest: first, the rise in antibiotic resistance by many bacterial pathogens has prompted interest in developing and using novel antibacterials; second, an increasing number of reliable s ... | 2016 | 27148246 |
| rapid identification and multiple susceptibility testing of pathogens from positive-culture sterile body fluids by a combined maldi-tof mass spectrometry and vitek susceptibility system. | infections of the bloodstream, central nervous system, peritoneum, joints, and other sterile areas are associated with high morbidity and sequelae risk. timely initiation of effective antimicrobial therapy is crucial to improving patient prognosis. however, standard final identification and antimicrobial susceptibility tests (asts) are reported 16-48 h after a positive alert. for a rapid, effective and low-cost diagnosis, we combined matrix-assisted laser desorption/ionization time of flight mas ... | 2016 | 27148212 |
| characterization of the gut microbiome using 16s or shotgun metagenomics. | the advent of next generation sequencing (ngs) has enabled investigations of the gut microbiome with unprecedented resolution and throughput. this has stimulated the development of sophisticated bioinformatics tools to analyze the massive amounts of data generated. researchers therefore need a clear understanding of the key concepts required for the design, execution and interpretation of ngs experiments on microbiomes. we conducted a literature review and used our own data to determine which ap ... | 2016 | 27148170 |
| genetic characterization of tem-type esbl-associated antibacterial resistance in enterobacteriaceae in a tertiary hospital in ghana. | antibiotic resistance due to the presence of extended-spectrum beta-lactamases (esbls) among enterobacteriaceae is a worldwide problem. data from ghana regarding this resistance mechanism is limited. this study was designed to investigate the presence of tem-type esbl genes, their locations and their conjugabilities in clinical isolates of enterobacteria collected from the korle-bu teaching hospital in ghana. | 2016 | 27145868 |
| bioprospecting sponge-associated microbes for antimicrobial compounds. | sponges are the most prolific marine organisms with respect to their arsenal of bioactive compounds including antimicrobials. however, the majority of these substances are probably not produced by the sponge itself, but rather by bacteria or fungi that are associated with their host. this review for the first time provides a comprehensive overview of antimicrobial compounds that are known to be produced by sponge-associated microbes. we discuss the current state-of-the-art by grouping the bioact ... | 2016 | 27144573 |
| co-spread of metal and antibiotic resistance within st3-inchi2 plasmids from e. coli isolates of food-producing animals. | concerns have been raised in recent years regarding co-selection for antibiotic resistance among bacteria exposed to heavy metals, particularly copper and zinc, used as growth promoters for some livestock species. in this study, 25 inchi2 plasmids harboring oqxab (20/25)/blactx-m (18/25) were found with sizes ranging from ∼260 to ∼350 kb and 22 belonged to the st3-inchi2 group. in addition to blactx-m and oqxab, pcoa-e (5/25) and sile-p (5/25), as well as aac(6')-ib-cr (18/25), flor (16/25), rmt ... | 2016 | 27143648 |
| antibacterial and antibiotic-modulation activity of six cameroonian medicinal plants against gram-negative multi-drug resistant phenotypes. | bacterial infections involving multi-drug resistant (mdr) phenotypes constitute a worldwide health concern. the present work was designed to assess the antibacterial properties of the methanol extracts of six medicinal plants (anthocleista schweinfurthii, nauclea latifolia, boehmeria platyphylla, caucalis melanantha, erigeron floribundus and zehneria scobra) and the effects of their associations with antibiotics on mdr gram-negative bacteria over-expressing active efflux pumps. | 2016 | 27142515 |
| antimicrobial activity of synthesized 2-methylthiobenzo[g][1,2,4]- triazolo[1,5-a]quinazoline derivatives. | the present study was carried out to evaluate the antimicrobial activity of a synthesized 2-methylthio-benzo[g][1,2,4]triazolo[1,5- a]quinazoline series. the compounds (1-21) were tested against a variety of gram-positive bacterial species including bacillus subtilis (rcmb 01001 69-3), enterococcus faecalis (rcmb 0100154-2), staphylococcus aureus (rcmb 0100183-9), staphylococcus epidermidis (rcmb 0100183-9) and streptococcus pyogenes (rcmb 0100172-5). in addition, gram-negative bacteria were als ... | 2016 | 27140181 |
| detection of carbapenemase genes in aquatic environments in rio de janeiro, brazil. | this study reveals the presence of different carbapenemase genes (blakpc, blandm, blages, and blaoxa48-like genes) detected directly from water samples and clonal dispersion (by pulsed-field gel electrophoresis [pfge] and multilocus sequence typing [mlst]) of kpc-2-producing enterobacteriaceae in two important urban aquatic matrixes from rio de janeiro, brazil, highlighting the role of aquatic environments as gene pools and the possibility of community spreading. | 2016 | 27139469 |
| sideromimic modification of lactivicin dramatically increases potency against extensively drug-resistant stenotrophomonas maltophilia clinical isolates. | acetamido derivatives of the naturally antibacterial non-β-lactam lactivicin (ltv) have improved activity against their penicillin binding protein targets and reduced hydrolysis by β-lactamases, but penetration into gram-negative bacteria is still relatively poor. here we report that modification of the ltv lactone with a catechol-type siderophore increases potency 1,000-fold against stenotrophomonas maltophilia, a species renowned for its insusceptibility to antimicrobials. the mic90 of modifie ... | 2016 | 27139464 |
| circulating of cmy-2 β-lactamase gene in weaned pigs and their environment in a commercial farm and the effect of feed supplementation with a clay mineral. | to investigate the mechanisms leading to an increase in the prevalence of blacmy -2 conferring resistance to ceftiofur in pigs receiving a feed medicated with chlortetracycline and penicillin, and to examine the effect of supplementation with a clay mineral on this phenomenon. | 2016 | 27138244 |
| a snapshot of co-resistance to carbapenems and tigecycline in clinical isolates of enterobacter cloacae. | enterobacter cloacae is one of the most common carbapenem-resistant enterobacteriaceae (cre) global wide. resistance to tigecycline, one of the few therapeutic options for cre infections, in carbapenem-resistant e. cloacae is of clinical significance. fourteen e. cloacae clinical isolates (ec1-ec14) co-resistant to tigecycline and carbapenems were studied. two tigecycline-susceptible/carbapenem-resistant isolates (ts1-ts2) were used for comparison. genotyping by pulsed-field gel electrophoresis ... | 2017 | 27136494 |
| refined spruce resin to treat chronic wounds: rebirth of an old folkloristic therapy. | significance: the treatment of chronic wounds results in an enormous drain on healthcare resources in terms of workload, costs, frustration, and impaired quality of life, and it presents a clinical challenge for physicians worldwide. effective local treatment of a chronic wound has an important role, particularly in patients who are-because of their poor general condition, diminished life expectancy, or unacceptable operative risk-outside of surgical treatment. recent advances: since 2002, our m ... | 2016 | 27134764 |
| structure and gene cluster of the o-antigen of enterobacter cloacae g3421. | the o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of enterobacter cloacae g3421 and studied by sugar analysis along with 1d and 2d (1)h and (13)c nmr spectroscopy. in addition, partial solvolysis with anhydrous trifluoroacetic acid was applied, which cleaved selectively the α-l-rhamnopyranosidic linkages. the following structure of the branched hexasaccharide repeating unit was established. the o-polysaccharide studied shares the β-l-rhap-(1→4)-α-l-rhap-(1→2)-α ... | 2016 | 27131290 |
| antibacterial activity of blue light against nosocomial wound pathogens growing planktonically and as mature biofilms. | the blue wavelengths within the visible light spectrum are intrinisically antimicrobial and can photodynamically inactivate the cells of a wide spectrum of bacteria (gram positive and negative) and fungi. furthermore, blue light is equally effective against both drug-sensitive and -resistant members of target species and is less detrimental to mammalian cells than is uv radiation. blue light is currently used for treating acnes vulgaris and helicobacter pylori infections; the utility for deconta ... | 2016 | 27129967 |
| polysaccharide-capped silver nanoparticles inhibit biofilm formation and eliminate multi-drug-resistant bacteria by disrupting bacterial cytoskeleton with reduced cytotoxicity towards mammalian cells. | development of effective anti-microbial therapeutics has been hindered by the emergence of bacterial strains with multi-drug resistance and biofilm formation capabilities. in this article, we report an efficient green synthesis of silver nanoparticle (agnp) by in situ reduction and capping with a semi-synthetic polysaccharide-based biopolymer (carboxymethyl tamarind polysaccharide). the cmt-capped agnps were characterized by uv, dls, fe-sem, edx and hr-tem. these agnps have average particle size ... | 2016 | 27125749 |
| liver abscess formation following transarterial chemoembolization: clinical features, risk factors, bacteria spectrum, and percutaneous catheter drainage. | to investigate the clinical features, risk factors, and bacterial spectrum of liver abscess following transarterial chemoembolization (tace) and evaluate the therapeutic effect of percutaneous catheter drainage (pcd) on the abscesses.a retrospective review of patient charts was performed in 3613 patients who suffered from liver malignancies (2832 patients with hepatocellular carcinoma and 781 with metastatic hepatic tumor) and had undergone 11,054 tace procedures from january 2005 to october 201 ... | 2016 | 27124055 |
| spread of tem, vim, shv, and ctx-m β-lactamases in imipenem-resistant gram-negative bacilli isolated from egyptian hospitals. | carbapenem-resistant gram-negative bacilli resulting from β-lactamases have been reported to be an important cause of nosocomial infections and are a critical therapeutic problem worldwide. this study aimed to describe the prevalence of imipenem-resistant gram-negative bacilli isolates and detection of bla vim, bla tem, bla shv, bla ctx-m-1, and bla ctx-m-9 genes in these clinical isolates in egyptian hospitals. the isolates were collected from various clinical samples, identified by conventiona ... | 2016 | 27123005 |
| use of shotgun metagenome sequencing to detect fecal colonization with multidrug-resistant bacteria in children. | prevention of multidrug-resistant (mdr) bacterial infections relies on accurate detection of these organisms. we investigated shotgun metagenome sequencing for the detection of methicillin-resistant staphylococcus aureus (mrsa), vancomycin-resistant enterococcus (vre), and mdr enterobacteriaceae fecal metagenomes were analyzed from high-risk inpatients and compared to those of low-risk outpatients and controls with minimal risk for a mdr bacterial infection. principal-component analysis clustere ... | 2016 | 27122381 |
| multisite evaluation of cepheid xpert carba-r assay for detection of carbapenemase-producing organisms in rectal swabs. | rapid identification of patients who are colonized with carbapenemase-producing organisms (cpo) is included in multiple national guidelines for containment of these organisms. in a multisite study, we evaluated the performance of the cepheid xpert carba-r assay, a qualitative diagnostic test that was designed for the rapid detection and differentiation of the blakpc, blandm, blavim, blaoxa-48, and blaimp-1 genes from rectal swab specimens. a double rectal swab set was collected from 383 patients ... | 2016 | 27122379 |
| evidence from a new york city hospital of rising incidence of genetically diverse carbapenem-resistant enterobacter cloacae and dominance of st171, 2007-14. | 2016 | 27118776 | |
| survey of clinical extended-spectrum beta-lactamase-producing enterobacter cloacae isolates in a chinese tertiary hospital, 2012-2014. | a total of 72 consecutive and nonduplicate clinical extended-spectrum β-lactamase (esbl)-producing enterobacter cloacae isolates were collected from our hospital from 2012 to 2014 for analyzing the prevalence of plasmid-mediated quinolone resistance (pmqr) genes, 16s rrna methyltransferase (16s-rmtase) encoding genes, and carbapenem-hydrolyzing β-lactamase (chβl) genes, as well as integrons. pulsed-field gel electrophoresis (pfge) and multilocus sequence typing (mlst) were carried out to charact ... | 2017 | 27116016 |
| dermatomyositis and polymyositis in the intensive care unit: a single-center retrospective cohort study of 102 patients. | patients with idiopathic inflammatory myopathies (iims) are sometimes complicated with life-threatening conditions requiring intensive care unit (icu) admission. in the past, owing to the low incidence of iim, little was known about such patients. our aim was to investigate the clinical features and outcomes of these patients and identify their risk factors for mortality. | 2016 | 27115138 |
| mechanisms involved in acquisition of blandm genes by inca/c2 and incfiiy plasmids. | blandm genes confer carbapenem resistance and have been identified on transferable plasmids belonging to different incompatibility (inc) groups. here we present the complete sequences of four plasmids carrying a blandm gene, pkp1-ndm-1, pec2-ndm-3, pecl3-ndm-1, and pec4-ndm-6, from four clinical samples originating from four different patients. different plasmids carry segments that align to different parts of the blandm region found on acinetobacter plasmids. pkp1-ndm-1 and pec2-ndm-3, from kle ... | 2016 | 27114281 |
| molecular diversity and plasmid analysis of kpc-producing escherichia coli. | the emergence and spread of klebsiella pneumoniae carbapenemase (kpc) among enterobacteriaceae presents a major public health threat to the world. although not as common as in k. pneumoniae, kpc is also found in escherichia coli strains. here, we genetically characterized 9 carbapenem-resistant e. coli strains isolated from six hospitals in the united states and completely sequenced their blakpc-harboring plasmids. the nine strains were isolated from different geographical locations and belonged ... | 2016 | 27114279 |
| antimicrobial activities of ceftazidime-avibactam and comparator agents against gram-negative organisms isolated from patients with urinary tract infections in u.s. medical centers, 2012 to 2014. | a total of 7,272 unique patient clinical isolates were collected from 71 u.s. medical centers from patients with urinary tract infections in 2012 to 2014 and tested for susceptibility to ceftazidime-avibactam and comparators by broth microdilution methods. ceftazidime-avibactam inhibited >99.9% of all enterobacteriaceae at the susceptible breakpoint of ≤8 μg/ml (there were only three nonsusceptible strains). ceftazidime-avibactam was also active against pseudomonas aeruginosa isolates (mic50, 2 ... | 2016 | 27114273 |
| enterobacter cloacae inhibits human norovirus infectivity in gnotobiotic pigs. | human noroviruses (hunovs) are the leading cause of epidemic gastroenteritis worldwide. study of hunov biology has been hampered by the lack of an efficient cell culture system. recently, enteric commensal bacteria enterobacter cloacae has been recognized as a helper in hunov infection of b cells in vitro. to test the influences of e. cloacae on hunov infectivity and to determine whether hunov infects b cells in vivo, we colonized gnotobiotic pigs with e. cloacae and inoculated pigs with 2.74 × ... | 2016 | 27113278 |
| phage-mediated shiga toxin (stx) horizontal gene transfer and expression in non-shiga toxigenic enterobacter and escherichia coli strains. | enterobacter cloacae m12x01451 strain recently identified from a clinical specimen produces a new stx1 subtype (stx1e) that was not neutralized by existing anti-stx1 monoclonal antibodies. acquisition of stx by ent. cloacae is rare and origin/stability of stx1e in m12x01451 is not known. in this study, we confirmed the ability of stx1a- and stx1e-converting phages from an escherichia coli o157:h7 strain rm8530 and m12x01451 respectively to infect several e. coli and ent. cloacae strains. stx1e w ... | 2016 | 27109772 |
| carbapenemase-producing enterobacteriaceae in hospital wastewater: a reservoir that may be unrelated to clinical isolates. | carbapenemase-producing enterobacteriaceae (cpe) are an emerging infection control problem in hospitals worldwide. identifying carriers may help reduce potential spread and infections. | 2016 | 27107615 |
| isolation of an aptamer that binds specifically to e. coli. | escherichia coli is a bacterial species found ubiquitously in the intestinal flora of animals, although pathogenic variants cause major public health problems. aptamers are short oligonucleotides that bind to targets with high affinity and specificity, and have great potential for use in diagnostics and therapy. we used cell-based systematic evolution of ligands by exponential enrichment (cell-selex) to isolate four single stranded dna (ssdna) aptamers that bind strongly to e. coli cells (atcc g ... | 2016 | 27104834 |
| ceftazidime-avibactam activity against aerobic gram negative organisms isolated from intra-abdominal infections in united states hospitals, 2012-2014. | ceftazidime-avibactam is ceftazidime combined with the novel non-β-lactam β-lactamase inhibitor avibactam, which inhibits ambler class a (e.g., extended-spectrum β-lactamase [esbl] and kpc), class c, and some class d enzymes. we evaluated the activity of ceftazidime-avibactam against aerobic gram negative bacteria causing intra-abdominal infections (iai). | 2016 | 27104633 |
| interleukin-6 kinetics can be useful for early treatment monitoring of severe bacterial sepsis and septic shock. | early appropriate anti-microbial therapy is necessary to improve outcomes of septic patients. we describe 20 case histories of patients with severe bacterial sepsis regarding kinetics of several biomarkers. we found that interleukin-6 is able to predict survival and might be able to evaluate appropriateness of anti-microbial therapy. | 2016 | 27103972 |
| new structures simultaneously harboring class 1 integron and iscr1-linked resistance genes in multidrug-resistant gram-negative bacteria. | the connection structure of class 1 integron and insertion sequence common region 1 (iscr1) is called "complex class 1 integrons" or "complex sul1-type integrons", which is also known to be associated with many resistance genes. this structure is a powerful gene-capturing tool kit that can mobilize antibiotic resistance genes. in order to look for and study the structure among clinical multidrug-resistant (mdr) gram-negative isolates, 63 isolates simultaneously harbored class 1 integron and iscr ... | 2016 | 27103443 |
| muscles provide protection during microbial infection by activating innate immune response pathways in drosophila and zebrafish. | muscle contraction brings about movement and locomotion in animals. however, muscles have also been implicated in several atypical physiological processes including immune response. the role of muscles in immunity and the mechanism involved has not yet been deciphered. in this paper, using drosophila indirect flight muscles (ifms) as a model, we show that muscles are immune-responsive tissues. flies with defective ifms are incapable of mounting a potent humoral immune response. upon immune chall ... | 2016 | 27101844 |
| mechanisms of ertapenem resistance in enterobacteriaceae isolates in a tertiary university hospital. | the aim of this study was to investigate the molecular mechanisms of ertapenem resistance among enterobacteriaceae isolates in a clinical microbiology laboratory at a tertiary university hospital. a total of 40 clinical isolates including 20 resistant and 20 intermediate isolates were collected from august 2012 to july 2013. ertapenem susceptibility was confirmed by the broth microdilution method. pcr and sequencing analysis of carbapenemase, ampc β-lactamase, and extended-spectrum β-lactamase ( ... | 2016 | 27101841 |
| intrawound vancomycin powder for spine tumor surgery. | study design retrospective evaluation of prospectively collected data. objective to evaluate infection rates following intrawound vancomycin powder application during spine tumor surgery. methods patients ≥18 years old undergoing spine tumor surgery and receiving intrawound vancomycin powder at a single center between january 2008 and january 2015 were enrolled. patient demographics (age, sex, body mass index [bmi]), tumor type (metastatic, primary) and location, surgical data (estimated blood l ... | 2015 | 27099810 |
| intrawound vancomycin powder for spine tumor surgery. | study design retrospective evaluation of prospectively collected data. objective to evaluate infection rates following intrawound vancomycin powder application during spine tumor surgery. methods patients ≥18 years old undergoing spine tumor surgery and receiving intrawound vancomycin powder at a single center between january 2008 and january 2015 were enrolled. patient demographics (age, sex, body mass index [bmi]), tumor type (metastatic, primary) and location, surgical data (estimated blood l ... | 2015 | 27099810 |
| human interleukin-2 and hen egg white lysozyme: screening for bacteriolytic activity against various bacterial cells. | the bacteriolytic activity of interleukin-2 and hen egg white lysozyme against 34 different species of microorganisms has been studied. it was found that 6 species of microorganisms are lysed in the presence of interleukin-2. all interleukin-2-sensitive microorganisms belong either to the enterobacteriaceae, bacillaceae, or the lactobacillaceae family. it was also found that 12 species of microorganisms are lysed in the presence of lysozyme, and 16 species of microorganisms are lysed in the pres ... | 2016 | 27099789 |
| primer evaluation for pcr and its application for detection of carbapenemases in enterobacteriaceae. | during the last decade, the prevalence of carbapenem-resistant enterobacteriaceae in human patients has increased. carbapenemase-producing bacteria are usually multidrug resistant. therefore, early recognition of carbapenemase producers is critical to prevent their spread. | 2016 | 27099689 |
| extensively drug-resistant bacteria are an independent predictive factor of mortality in 130 patients with spontaneous bacterial peritonitis or spontaneous bacteremia. | to evaluate the epidemiology and outcomes of culture-positive spontaneous bacterial peritonitis (sbp) and spontaneous bacteremia (sb) in decompensated cirrhosis. | 2016 | 27099449 |
| noncanonical dna-binding mode of repressor and its disassembly by antirepressor. | dna-binding repressors are involved in transcriptional repression in many organisms. disabling a repressor is a crucial step in activating expression of desired genes. thus, several mechanisms have been identified for the removal of a stably bound repressor (rep) from the operator. here, we describe an uncharacterized mechanism of noncanonical dna binding and induction by a rep from the temperate salmonella phage spc32h; this mechanism was revealed using the crystal structures of homotetrameric ... | 2016 | 27099293 |
| strain-level dissection of the contribution of the gut microbiome to human metabolic disease. | the gut microbiota has been linked with metabolic diseases in humans, but demonstration of causality remains a challenge. the gut microbiota, as a complex microbial ecosystem, consists of hundreds of individual bacterial species, each of which contains many strains with high genetic diversity. recent advances in genomic and metabolomic technologies are facilitating strain-level dissection of the contribution of the gut microbiome to metabolic diseases. interventional studies and correlation anal ... | 2016 | 27098841 |
| the sandfly lutzomyia longipalpis ll5 embryonic cell line has active toll and imd pathways and shows immune responses to bacteria, yeast and leishmania. | lutzomyia longipalpis is the main vector of visceral leishmaniasis in latin america. sandfly immune responses are poorly understood. in previous work we showed that these vector insects respond to bacterial infections by modulating a defensin gene expression and activate the imd pathway in response to leishmania infection. aspects of innate immune pathways in insects (including mosquito vectors of human diseases) have been revealed by studying insect cell lines, and we have previously demonstrat ... | 2016 | 27098567 |
| retrospective analysis of the microbiological spectrum of pneumonia in turkish patients with lung cancer. | the spectrum of pulmonary infections in patients with lung cancer is wide, and tools for target-oriented infection control measures are necessary. in this retrospective study we report the microbiological spectrum of pneumonia (based on the results of sputum culture) in a case series of turkish patients with lung malignancies. | 2016 | 27095942 |
| evaluation of a fully automated research prototype for the immediate identification of microorganisms from positive blood cultures under clinical conditions. | a clinical laboratory evaluation of an intrinsic fluorescence spectroscopy (ifs)-based identification system paired to a bact/alert virtuo microbial detection system (biomérieux, inc., durham, nc) was performed to assess the potential for fully automated identification of positive blood cultures. the prototype ifs system incorporates a novel method combining a simple microbial purification procedure with rapid in situ identification via spectroscopy. results were available within 15 min of a bot ... | 2016 | 27094332 |
| duplex dna-invading γ-modified peptide nucleic acids enable rapid identification of bloodstream infections in whole blood. | bloodstream infections are a leading cause of morbidity and mortality. early and targeted antimicrobial intervention is lifesaving, yet current diagnostic approaches fail to provide actionable information within a clinically viable time frame due to their reliance on blood culturing. here, we present a novel pathogen identification (pid) platform that features the use of duplex dna-invading γ-modified peptide nucleic acids (γpnas) for the rapid identification of bacterial and fungal pathogens di ... | 2016 | 27094328 |
| isolation and characterization of aquatic-borne klebsiella pneumoniae from tropical estuaries in malaysia. | klebsiella pneumoniae is an opportunistic pathogen that is responsible for causing nosocomial and community-acquired infections. despite its common presence in soil and aquatic environments, the virulence potential of k. pneumoniae isolates of environmental origin is largely unknown. hence, in this study, k. pneumoniae isolated from the estuarine waters and sediments of the matang mangrove estuary were screened for potential virulence characteristics: antibiotic susceptibility, morphotype on con ... | 2016 | 27092516 |
| expression of zinc transporter genes in rice as influenced by zinc-solubilizing enterobacter cloacae strain zsb14. | zinc (zn) deficiency in major food crops has been considered as an important factor affecting the crop production and subsequently the human health. rice (oryza sativa) is sensitive to zn deficiency and thereby causes malnutrition to most of the rice-eating asian populations. application of zinc solubilizing bacteria (zsb) could be a sustainable agronomic approach to increase the soil available zn which can mitigate the yield loss and consequently the nutritional quality of rice. understanding t ... | 2016 | 27092162 |
| high prevalence of multidrug resistant enterobacteriaceae isolated from outpatient urine samples but not the hospital environment in bo, sierra leone. | the rising level of antimicrobial resistance among bacterial pathogens is one of the most significant public health problems globally. while the antibiotic resistance of clinically important bacteria is closely tracked in many developed countries, the types and levels of resistance and multidrug resistance (mdr) among pathogens currently circulating in most countries of sub-saharan africa are virtually unknown. | 2016 | 27090787 |
| clinical efficacy of polymyxin monotherapy versus nonvalidated polymyxin combination therapy versus validated polymyxin combination therapy in extensively drug-resistant gram-negative bacillus infections. | polymyxins have emerged as a last-resort treatment of extensively drug-resistant (xdr) gram-negative bacillus (gnb) infections, which present a growing threat. individualized polymyxin-based antibiotic combinations selected on the basis of the results of in vitro combination testing may be required to optimize therapy. a retrospective cohort study of hospitalized patients receiving polymyxins for xdr gnb infections from 2009 to 2014 was conducted to compare the treatment outcomes between patient ... | 2016 | 27090177 |
| comparison of methods to identify pathogens and associated virulence functional genes in biosolids from two different wastewater treatment facilities in canada. | the use of treated municipal wastewater residues (biosolids) as fertilizers is an attractive, inexpensive option for growers and farmers. various regulatory bodies typically employ indicator organisms (fecal coliforms, e. coli and salmonella) to assess the adequacy and efficiency of the wastewater treatment process in reducing pathogen loads in the final product. molecular detection approaches can offer some advantages over culture-based methods as they can simultaneously detect a wider microbia ... | 2016 | 27089040 |
| clinical impact of methicillin-resistant staphylococcus aureus on bacterial pneumonia: cultivation and 16s ribosomal rna gene analysis of bronchoalveolar lavage fluid. | determining whether methicillin-resistant staphylococcus aureus (mrsa) is a true causative pathogen or reflective of colonization when mrsa is cultured from the respiratory tract remains important in treating patients with pneumonia. | 2016 | 27083412 |
| structural insights into the escherichia coli lysine decarboxylases and molecular determinants of interaction with the aaa+ atpase rava. | the inducible lysine decarboxylase ldci is an important enterobacterial acid stress response enzyme whereas ldcc is its close paralogue thought to play mainly a metabolic role. a unique macromolecular cage formed by two decamers of the escherichia coli ldci and five hexamers of the aaa+ atpase rava was shown to counteract acid stress under starvation. previously, we proposed a pseudoatomic model of the ldci-rava cage based on its cryo-electron microscopy map and crystal structures of an inactive ... | 2016 | 27080013 |
| what does the susceptible-dose dependent interpretive category for cefepime in esbl-producing bacteria? | in this study, we investigated the frequency of isolates included in the susceptible-dose dependent (sdd) category, according to the clinical and laboratory standards institute guidelines, carrying blatem, blashv and blactx genes among 92 klebsiella pneumoniae and 80 enterobacter cloacae clinical isolates. the presence of one or more extended-spectrum β-lactamases (esbls) genes was observed in 64% k. pneumoniae and 69% e. cloacae isolates. nineteen isolates were included in sdd interpretive cate ... | 2016 | 27077929 |
| influence of culture media on detection of carbapenem hydrolysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | in this study, we evaluated the influence of distinct bacterial growth media on detection of carbapenemase hydrolysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. false-negative results were observed for oxa-25-, oxa-26-, and oxa-72-producing acinetobacter baumannii isolates grown on macconkey agar medium. the other culture media showed 100% sensitivity and 100% specificity for detecting carbapenemase. | 2016 | 27076665 |
| dissemination of the blakpc gene by clonal spread and horizontal gene transfer: comparative study of incidence and molecular mechanisms. | in addition to the global spread of the kpc-producing klebsiella pneumoniae (kpc-kp) clonal complex (cc)-258 clone, the blakpc gene may also spread by horizontal gene transfer (hgt), as suspected when more than one kpc-producing enterobacteriaceae (kpc-ent) species are isolated in a single patient. we aimed to characterize the incidence and molecular features of kpc-kp that were isolated alone (singular kpc-kp) versus kpc-kp that were isolated together with another kpc-ent species (joint kpc-kp) ... | 2016 | 27073266 |
| a glimpse into evolution and dissemination of multidrug-resistant acinetobacter baumannii isolates in east asia: a comparative genomics study. | clonal dissemination is characteristic of the important nosocomial pathogen acinetobacter baumannii, as revealed by previous multi-locus sequence typing (mlst) studies. however, the disseminated phyletic unit is actually mlst sequence type instead of real bacterial clone. here we sequenced the genomes of 13 multidrug-resistant (mdr) a. baumannii strains from taiwan, and compared them with that of a. baumannii from other east asian countries. core-genome phylogenetic tree divided the analyzed str ... | 2016 | 27072398 |
| treatment outcomes of oral sitafloxacin in acute complicated urinary tract infection and pyelonephritis. | data on the success rate of sitafloxacin treatment in acute complicated urinary tract infection and pyelonephritis are limited. | 2016 | 27069830 |
| nested russian doll-like genetic mobility drives rapid dissemination of the carbapenem resistance gene blakpc. | the recent widespread emergence of carbapenem resistance in enterobacteriaceae is a major public health concern, as carbapenems are a therapy of last resort against this family of common bacterial pathogens. resistance genes can mobilize via various mechanisms, including conjugation and transposition; however, the importance of this mobility in short-term evolution, such as within nosocomial outbreaks, is unknown. using a combination of short- and long-read whole-genome sequencing of 281 blakpc- ... | 2016 | 27067320 |
| impact of cefepime susceptible-dose-dependent mic for enterobacteriaceae on reporting and prescribing. | the clsi cefepime breakpoints were revised to include a susceptible-dose-dependent (sdd) category for enterobacteriaceae, with the intention that higher cefepime doses be used for these isolates. in this study, 1.6% of enterobacteriaceae isolates were reported as sdd, with escherichia coli the most common sdd organism. cefepime was prescribed in three cases (4.8%); the majority of sdd isolates were treated with a carbapenem. enterobacteriaceae with cefepime sdd mics were not commonly treated wit ... | 2016 | 27067319 |
| amino acid substitutions of crrb responsible for resistance to colistin through crrc in klebsiella pneumoniae. | colistin is a last-resort antibiotic for treatment of carbapenem-resistant klebsiella pneumoniae a recent study indicated that missense mutations in the crrb protein contribute to colistin resistance. in our previous study, mechanisms of colistin resistance were defined in 17 of 26 colistin-resistant k. pneumoniae clinical isolates. of the remaining nine strains, eight were highly resistant to colistin. in the present study, crrab sequences were determined for these eight strains. six separate a ... | 2016 | 27067316 |
| molecular epidemiology and genetic characteristics of various blaper genes in shanghai, china. | we describe the genetic characteristics and possible transmission mechanism of blaper in 25 clinical gram-negative bacilli in shanghai. blaper, including blaper-1, blaper-3, and blaper-4, was located chromosomally or in different plasmids. tn1213 harboring blaper-1 was first identified in two proteus mirabilis isolates in china. the other blaper variants were preceded by an iscr1 element inside the complex class 1 integron associated with is26, tn21, tn1696, and a miniature inverted-repeat trans ... | 2016 | 27067315 |
| species diversity of environmental gim-1-producing bacteria collected during a long-term outbreak. | reports of outbreaks concerning carbapenemase-producing gram-negative bacteria in which the main source of transmission is the hospital environment are increasing. this study describes the results of environmental sampling in a protracted polyspecies metallo-beta-lactamase gim-1 outbreak driven by plasmids and bacterial clones of enterobacter cloacae and pseudomonas aeruginosa in a tertiary care center. environmental sampling targeting wet locations (especially sinks) was carried out on a surgic ... | 2016 | 27060121 |
| the intestinal microbiota in metabolic disease. | gut bacteria exert beneficial and harmful effects in metabolic diseases as deduced from the comparison of germfree and conventional mice and from fecal transplantation studies. compositional microbial changes in diseased subjects have been linked to adiposity, type 2 diabetes and dyslipidemia. promotion of an increased expression of intestinal nutrient transporters or a modified lipid and bile acid metabolism by the intestinal microbiota could result in an increased nutrient absorption by the ho ... | 2016 | 27058556 |