Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| [construction of transgenic tobacco expressing popw and analysis of its biological phenotype]. | in a previous study, we cloned popw from ralstonia solanacearum strain zj3721, coding popw, a new harpin protein. the procaryotically expressed popw can induce resistance to tobacco mosaic virus (tmv), enhance growth and improve quality of tobacco, when sprayed onto tobacco leaves. here, we constructed an expression vector pb- popw by cloning popw into the bionary vector pbi121 and transformed it into agrobacterium tumefaciens strain eha105 via freeze-thaw method. tobacco (nicotiana tobacum cv. ... | 2014 | 25195247 |
| overexpression of atdreb1d transcription factor improves drought tolerance in soybean. | drought is one of the major abiotic stresses that affect productivity in soybean (glycine max l.) several genes induced by drought stress include functional genes and regulatory transcription factors. the arabidopsis thaliana dreb1d transcription factor driven by the constitutive and aba-inducible promoters was introduced into soybean through agrobacterium tumefaciens-mediated gene transfer. several transgenic lines were generated and molecular analysis was performed to confirm transgene integra ... | 2014 | 25192890 |
| application of rna interference methodology to investigate and develop scmv resistance in maize. | specific fragments of the sugarcane mosaic virus (scmv) coat protein gene (cp) were amplified by reverse transcriptionpolymerase chain reaction and used to construct a marker free small interfering rna complex expression vector against scmv. in planta transformation was performed on maize (zea mays) inbred line 8112 mediated by agrobacterium tumefaciens. pcr and southern blot analyses demonstrated successful integration of the cp segment into the 8112 genome. the in planta transformation frequen ... | 2014 | 25189224 |
| molecular characterization of ku70 and ku80 homologues and exploitation of a ku70-deficient mutant for improving gene deletion frequency in rhodosporidium toruloides. | rhodosporidium toruloides is a β-carotenoid accumulating, oleaginous yeast that has great biotechnological potential. the lack of reliable and efficient genetic manipulation tools have been a major hurdle blocking its adoption as a biotechnology platform. | 2014 | 25188820 |
| expression of a staphylokinase, a thrombolytic agent in arabidopsis thaliana. | a gene encoding staphylokinase from staphylococcus aureus was cloned into the plant transformation binary vector pcambia 1304. the transgene was introduced into the genome of a. thaliana via in planta agrobacterium tumefaciens-mediated genetic transformation. the presence of the staphylokinase gene was confirmed by pcr in 60% of the investigated plants. the presence of the fusion protein (119 kda) was confirmed by sds-page and western blot analysis in protein extracts from putative transgenics. ... | 2011 | 25187133 |
| inoculation of lens culinaris with pb-resistant bacteria shows potential for phytostabilization. | phytoremediation comprises a set of plant and microbe-based technologies for remediation of soil heavy metal contamination. in this work, four pb-resistant bacteria (agrobacterium tumefaciens, rahnella aquatilis, and two pseudomonas sp.) were selected among a collection of isolates from root nodule of lens culinaris. they had a high degree of bioaccumulation ability in nutrient medium containing 2 mm pb, and the maximum pb accumulation of whole cell was found after 48-h incubation. these pb-resi ... | 2015 | 25185494 |
| synthesis of methylerythritol phosphate analogues and their evaluation as alternate substrates for ispdf and ispe from agrobacterium tumefaciens. | the methylerythritol phosphate biosynthetic pathway, found in most bacteria, some parasitic protists, and plant chloroplasts, converts d-glyceraldehyde phosphate and pyruvate to isopentenyl diphosphate (ipp) and dimethylallyl diphosphate (dmapp), where it intersects with the mevalonate pathway found in some bacteria, archaea, and eukarya, including the cytosol of plants. d-3-methylerythritol-4-phosphate (mep), the first pathway-specific intermediate in the pathway, is converted to ipp and dmapp ... | 2014 | 25184438 |
| agrobacterium tumefaciens mediated transient expression of plant cell wall-degrading enzymes in detached sunflower leaves. | for biofuel applications, synthetic endoglucanase e1 and xylanase (xyn10a) derived from acidothermus cellulolyticus were transiently expressed in detached whole sunflower (helianthus annuus l.) leaves using vacuum infiltration. three different expression systems were tested, including the constitutive camv 35s-driven, cmvar (cucumber mosaic virus advanced replicating), and trbo (tobacco mosaic virus rna-based overexpression vector) systems. for 6-day leaf incubations, codon-optimized e1 and xyla ... | 2014 | 25180328 |
| cre reporter assay for translocation (craft): a tool for the study of protein translocation into host cells. | many pathogenic bacteria introduce virulence proteins, also called effector proteins, into host cells to accomplish infection. such effector proteins are often translocated into host cells by bacterial type iii (t3ss) or type iv secretion systems (t4ss). to better understand the molecular mechanisms underlying virulence, it is essential to identify the effector proteins and determine their functions. several reporter assays have been established to identify translocated effector proteins and ver ... | 2014 | 25172277 |
| overexpression of the hspl promotes agrobacterium tumefaciens virulence in arabidopsis under heat shock conditions. | agrobacterium tumefaciens transfers a specific dna fragment from the resident tumor-inducing (ti) plasmid and effector virulence (vir) proteins to plant cells during infection. a. tumefaciens virb1-11 and vird4 proteins assemble as the type iv secretion system (t4ss), which mediates transfer of the t-dna and effector vir protein into plant cells, thus resulting in crown gall disease in plants. previous studies revealed that an α-crystallin-type, small heat-shock protein (hspl) is a more effectiv ... | 2015 | 25163013 |
| historical account on gaining insights on the mechanism of crown gall tumorigenesis induced by agrobacterium tumefaciens. | the plant tumor disease known as crown gall was not called by that name until more recent times. galls on plants were described by malpighi (1679) who believed that these extraordinary growth are spontaneously produced. agrobacterium was first isolated from tumors in 1897 by fridiano cavara in napoli, italy. after this bacterium was recognized to be the cause of crown gall disease, questions were raised on the mechanism by which it caused tumors on a variety of plants. numerous very detailed stu ... | 2014 | 25147542 |
| sri lankan cassava mosaic virus replication associated protein (rep) triggers transposition of is426 in agrobacterium. | we report a high rate of is426 transposition in agrobacterium tumefaciens in the presence of the sri lankan cassava mosaic virus (slcmv) replication associated protein gene (rep). upon conjugal transfer of the binary plasmid pcam-slcmv-rep with the slcmv rep gene in the sense orientation under the transcriptional control of the cauliflower mosaic virus (camv) 35s promoter into the a. tumefaciens vir helper strain eha105, the binary plasmid size increased in all 15 transconjugants studied. southe ... | 2014 | 25135797 |
| the transcription factor vvwrky33 is involved in the regulation of grapevine (vitis vinifera) defense against the oomycete pathogen plasmopara viticola. | grapevine (vitis vinifera ssp. vinifera) is one of the most important fruit species; however, it is highly susceptible to various pathogens, which can cause severe crop losses in viticulture. it has been shown that several wrky class transcription factors (tfs) are part of the signal transduction cascade, which leads to the activation of plant defense reactions against various pathogens. in the present investigation, a full-length cdna was isolated from v. vinifera leaf tissue encoding a predict ... | 2015 | 25132131 |
| viral and chloroplastic signals essential for initiation and efficiency of translation in agrobacterium tumefaciens. | the construction of high-level protein expression vectors using the camv 35s promoter in concert with highly efficient translation initiation signals for agrobacterium tumefaciens is a relatively less explored field compared to that of escherichia coli. in the current study, we experimentally investigated the capacity of the camv 35s promoter to direct gfp gene expression in a. tumefaciens in the context of different viral and chloroplastic translation initiation signals. gfp expression and conc ... | 2014 | 25117444 |
| successful agrobacterium mediated transformation of thielaviopsis basicola by optimizing multiple conditions. | thielaviopsis basicola is a hemibiotrophic root pathogen causing black root rot in a wide range of economically important crops. our initial attempts to transform t. basicola using standard agrobacterium tumefaciens-mediated transformation (atmt) protocols were unsuccessful. successful transformation required the addition of v8 juice (to induce germination of t. basicola chlamydospores) and higher concentrations of acetosyringone in the co-cultivation medium, and of chlamydospores/endoconidia, a ... | 2014 | 25110130 |
| early-flowering sweet orange mutant 'x11' as a model for functional genomic studies of citrus. | there had been many reports on genetic transformation of citrus for functional genomic studies but few included genes associated with flower or fruit traits. a major reason for this might derive from the extensive juvenile stage of citrus plants when regenerated from juvenile explants (epicotyls, cotyledon or calli), which delays the observation of the resulting phenotype. alternatives include the use of explants from adult tissues, which sometimes may be recalcitrant to regeneration or transfor ... | 2014 | 25108567 |
| agrobacterium tumefaciens-mediated transformation in the entomopathogenic fungus lecanicillium lecanii and development of benzimidazole fungicide resistant strains. | lecanicillium lecanii has been used in the biological control of several insects in agricultural practice. since the gene manipulation tools for this entomopathogenic fungus have not been sufficiently developed, agrobacterium tumefaciens-mediated transformation (atmt) in l. lecanii was investigated in this study, using the wild-type isolate fz9906 as a progenitor strain and the hygromycin b resistance (hph) gene as a selection marker. furthermore, a field carbendazim-resistant (mrt) gene from bo ... | 2014 | 25107375 |
| discovery and validation of novel and distinct rna regulators for ribosomal protein s15 in diverse bacterial phyla. | autogenous cis-regulators of ribosomal protein synthesis play a critical role in maintaining the stoichiometry of ribosome components. structured portions within an mrna transcript typically interact with specific ribosomal proteins to prevent expression of the entire operon, thus balancing levels of ribosomal proteins across transcriptional units. three distinct rna structures from different bacterial phyla have demonstrated interactions with s15 to regulate gene expression; however, these rnas ... | 2014 | 25104606 |
| comparative investigation of the reaction mechanisms of the organophosphate-degrading phosphotriesterases from agrobacterium radiobacter (opda) and pseudomonas diminuta (oph). | metal ion-dependent, organophosphate-degrading enzymes have acquired increasing attention due to their ability to degrade and thus detoxify commonly used pesticides and nerve agents such as sarin. the best characterized of these enzymes are from pseudomonas diminuta (oph) and agrobacterium radiobacter (opda). despite high sequence homology (>90 % identity) and conserved metal ion coordination these enzymes display considerable variations in substrate specificity, metal ion affinity/preference an ... | 2014 | 25104333 |
| morphogenetic and chemical stability of long-term maintained agrobacterium-mediated transgenic catharanthus roseus plants. | transgenic catharanthus roseus plants (transgenic dhawal [dt] and transgenic nirmal [nt]) obtained from the agrobacterium tumefaciens and agrobacterium rhizognenes-mediated transformations, respectively, have been maintained in vitro for 5 years. plants were studied at regular intervals for various parameters such as plant height, leaf size, multiplication rate, alkaloid profile and presence of marker genes. dt plant gradually lost the gus gene expression and it was not detected in the fifth yea ... | 2015 | 25102992 |
| identification and functional analysis of penicillium digitatum genes putatively involved in virulence towards citrus fruit. | the fungus penicillium digitatum, the causal agent of green mould rot, is the most destructive post-harvest pathogen of citrus fruit in mediterranean regions. in order to identify p. digitatum genes up-regulated during the infection of oranges that may constitute putative virulence factors, we followed a polymerase chain reaction (pcr)-based suppression subtractive hybridization and cdna macroarray hybridization approach. the origin of expressed sequence tags (ests) was determined by comparison ... | 2015 | 25099378 |
| a comparison study of agrobacterium-mediated transformation methods for root-specific promoter analysis in soybean. | both in vitro and in vivo hairy root transformation systems could not replace whole plant transformation for promoter analysis of root-specific and low-p induced genes in soybean. an efficient genetic transformation system is crucial for promoter analysis in plants. agrobacterium-mediated transformation is the most popular method to produce transgenic hairy roots or plants. in the present study, first, we compared the two different agrobacterium rhizogenes-mediated hairy root transformation meth ... | 2014 | 25097075 |
| [establishment of transformation system of lonicera macranthoides mediated by agrobacterium tumefaciens]. | based on the system of regeneration,the genetic transformation system of lonicera macranthoides was established. | 2013 | 25090668 |
| multiplex pcr assay for identification of commonly used disarmed agrobacterium tumefaciens strains. | the success of agrobacterium mediated plant transformation depends to a certain extent on appropriate selection of the a. tumefaciens strain for a particular plant species. many stages in a plant transformation procedure are prone to bacterial contamination with similar antibiotic resistance that may compromise the identity of the a. tumefaciens strain used, in turn adversely affecting success of a transformation experiment. different primer sets were designed to exploit genetic differences amon ... | 2014 | 25089249 |
| heterologous expression of vhb can improve the yield and quality of biocontrol fungus paecilomyces lilacinus, during submerged fermentation. | paecilomyces lilacinus is an egg-parasitic fungus which is effective against plant-parasitic nematodes and it has been successfully commercialized for the control of many plant-parasitic nematodes. however, during the large-scale industrial fermentation process of the filamentous fungus, the dissolved oxygen supply is a limiting factor, which influences yield, product quality and production cost. to solve this problem, we intended to heterologously express vhb in p. lilacinus acss. after optimiz ... | 2014 | 25087739 |
| rnai mediated gene silencing against betasatellite associated with croton yellow vein mosaic begomovirus. | plant viruses encode suppressors of posttranscriptional gene silencing, an adaptive antiviral defense responses that confines virus infection. previously, we identified single-stranded dna satellite (also known as dna-β) of ~1,350 nucleotides in length associated with croton yellow vein mosaic begomovirus (cyvmv) in croton plants. the expression of genes from dna-β requires the begomovirus for packaged, replication, insect transmission and movement in plants. the present study demonstrates the e ... | 2014 | 25086625 |
| agroinfiltration as an effective and scalable strategy of gene delivery for production of pharmaceutical proteins. | current human biologics are most commonly produced by mammalian cell culture-based fermentation technologies. however, its limited scalability and high cost prevent this platform from meeting the ever increasing global demand. plants offer a novel alternative system for the production of pharmaceutical proteins that is more scalable, cost-effective, and safer than current expression paradigms. the recent development of deconstructed virus-based vectors has allowed rapid and high-level transient ... | 0 | 25077181 |
| agrobacterium tumefaciens responses to plant-derived signaling molecules. | as a special phytopathogen, agrobacterium tumefaciens infects a wide range of plant hosts and causes plant tumors also known as crown galls. the complexity of agrobacterium-plant interaction has been studied for several decades. agrobacterium pathogenicity is largely attributed to its evolved capabilities of precise recognition and response to plant-derived chemical signals. agrobacterium perceives plant-derived signals to activate its virulence genes, which are responsible for transferring and ... | 2014 | 25071805 |
| repercussion of mesophyll-specific overexpression of a soybean cytosolic glutamine synthetase gene in alfalfa (medicago sativa l.) and tobacco (nicotiana tabaccum l.). | glutamine synthetase (gs) plays a central role in plant nitrogen metabolism. plant gs occurs as a number of isoenzymes present in either the cytosol (gs1) or chloroplast/plastid (gs2). there are several reports of improved performance in transgenic plants overexpressing gs1 transgenes driven by the constitutive camv35s promoter. improvement has been attributed to the gs1 transgene product functioning to enhance re-assimilation of nh4(+) released by photorespiration or protein degradation. in thi ... | 2009 | 25067868 |
| agroinfiltration by cytokinin-producing agrobacterium sp. strain gv3101 primes defense responses in nicotiana tabacum. | transient infiltrations in tobacco are commonly used in plant studies, but the host response to different disarmed agrobacterium strains is not fully understood. the present study shows that pretreatment with disarmed agrobacterium tumefaciens gv3101 primes the defense response to subsequent infection by pseudomonas syringae in nicotiana tabacum. the presence of a trans-zeatin synthase (tzs) gene in strain gv3101 may be partly responsible for the priming response, as the tzs-deficient agrobacter ... | 2014 | 25054409 |
| 6-hydroxy-3-succinoylpyridine hydroxylase catalyzes a central step of nicotine degradation in agrobacterium tumefaciens s33. | nicotine is a main alkaloid in tobacco and is also the primary toxic compound in tobacco wastes. it can be degraded by bacteria via either pyridine pathway or pyrrolidine pathway. previously, a fused pathway of the pyridine pathway and the pyrrolidine pathway was proposed for nicotine degradation by agrobacterium tumefaciens s33, in which 6-hydroxy-3-succinoylpyridine (hsp) is a key intermediate connecting the two pathways. we report here the purification and properties of an nadh-dependent hsp ... | 2014 | 25054198 |
| distribution and diversity of rhizobia associated with wild soybean (glycine soja sieb. & zucc.) in northwest china. | a total of 155 nodule isolates that originated from seven sites in northwest china were characterized by pcr-rflp of the 16s rrna gene and sequence analysis of multiple core genes (16s rrna, reca, atpd, and glnii) in order to investigate the diversity and biogeography of glycine soja-nodulating rhizobia. among the isolates, 80 were ensifer fredii, 19 were ensifer morelense, 49 were rhizobium radiobacter, and 7 were putative novel rhizobium species. the phylogenies of e. fredii and e. morelense i ... | 2014 | 25052953 |
| antitumor properties of two traditional aromatic rice genotypes (kalijira and chinigura). | methanol extract of bran and unpolished grain of two traditional aromatic rice genotypes viz. kalijira and chinigura were assayed for their activity on the growth and initiation of crown-gall tumors on potato disks. | 2014 | 25050299 |
| transgenic expression of mshsp23 confers enhanced tolerance to abiotic stresses in tall fescue. | tall fescue (festuca arundinacea schreb.) is an important cool season forage plant that is not well suited to extreme heat, salts, or heavy metals. to develop transgenic tall fescue plants with enhanced tolerance to abiotic stress, we introduced an alfalfa hsp23 gene expression vector construct through agrobacterium-mediated transformation. integration and expression of the transgene were confirmed by polymerase chain reaction, northern blot, and western blot analyses. under normal growth condit ... | 2012 | 25049632 |
| genetic transformation of fusarium avenaceum by agrobacterium tumefaciens mediated transformation and the development of a user-brick vector construction system. | the plant pathogenic and saprophytic fungus fusarium avenaceum causes considerable in-field and post-field losses worldwide due to its infections of a wide range of different crops. despite its significant impact on the profitability of agriculture production and a desire to characterize the infection process at the molecular biological level, no genetic transformation protocol has yet been established for f. avenaceum. in the current study, it is shown that f. avenaceum can be efficiently trans ... | 2014 | 25048842 |
| the multifaceted roles of the interspecies signalling molecule indole in agrobacterium tumefaciens. | bacteria utilize signal molecules to ensure their survival in environmental niches, and indole is an interspecies and interkingdom signalling molecule, which is widespread in the natural environment. in this study, we sought to identify novel roles of indole in soil-borne bacterium agrobacterium tumefaciens. agrobacterium tumefaciens was found not to synthesize indole and to degrade it rapidly. the addition of exogenous indole dose-dependently inhibited a. tumefaciens growth and decreased its mo ... | 2015 | 25040348 |
| screening of high toxic metarhizium strain against plutella xylostella and its marking with green fluorescent protein. | entomopathogenic fungus is proposed to be one of the best biocontrol agents against the destructive insect pest plutella xylostella. in this study, we tested the virulence of 11 metarhizium strain isolates against p. xylostella using a leaf dipping method, and found one strain, named 609, which had displayed the highest pathogenicity. bioassay results showed that the accumulated corrected mortality rate was 86.7 % on the eighth day after inoculation with a spore concentration 1 × 10(8) conidia/m ... | 2014 | 25037866 |
| antimicrobial and inhibitory enzyme activity of n-(benzyl) and quaternary n-(benzyl) chitosan derivatives on plant pathogens. | chemical modification of a biopolymer chitosan by introducing quaternary ammonium moieties into the polymer backbone enhances its antimicrobial activity. in the present study, a series of quaternary n-(benzyl) chitosan derivatives were synthesized and characterized by (1)h-nmr, ft-ir and uv spectroscopic techniques. the antimicrobial activity against crop-threatening bacteria agrobacterium tumefaciens and erwinia carotovora and fungi botrytis cinerea, botryodiplodia theobromae, fusarium oxysporu ... | 2014 | 25037402 |
| protoplast: a more efficient system to study nucleo-cytoplasmic interactions. | agrobacterium tumefaciens-mediated genetic transformation is a powerful tool for plant research, but it can be labor-intensive and time-consuming. here, we report a protoplast-based approach to study nucleo-cytoplasmic interactions, such as cytoplasmic male sterility/fertility restoration (cms/rf) and organellar rna editing. to test the system, we transfected the fertility restorer gene rf5, which is involved in the rice hl-cms/rf system, into rice protoplasts prepared from the hl-cms line. as t ... | 2014 | 25026554 |
| [construction of plant expression vectors with pmi gene as selection marker and their utilization in transformation of salvia miltiorrhiza f. alba]. | to construct plant expression pcambia1301-pmi by substituting pmi for hygromycin resistance gene in pcambia1301 and obtain transgenic salvia miltiorrhiza f. alba using pmi-mannose selection system. | 2014 | 25011255 |
| type vi secretion system helps find a niche. | type vi secretion systems (t6sss) deliver toxins into target cells and thus play a role in bacterial warfare. in this issue of cell host & microbe, ma et al. (2014) demonstrate that t6ss-dependent attack during interbacterial competition in the host context enables niche colonization by agrobacterium tumefaciens. | 2014 | 25011102 |
| the role of the de novo pyrimidine biosynthetic pathway in cryptococcus neoformans high temperature growth and virulence. | fungal infections are often difficult to treat due to the inherent similarities between fungal and animal cells and the resulting host toxicity from many antifungal compounds. cryptococcus neoformans is an opportunistic fungal pathogen of humans that causes life-threatening disease, primarily in immunocompromised patients. since antifungal therapy for this microorganism is limited, many investigators have explored novel drug targets aim at virulence factors, such as the ability to grow at mammal ... | 2014 | 25011011 |
| [subcellular localization and resistance to gibberella fujikuroi of atelhyprp2 in transgenic tobacco]. | the subcellular localization and the resistance to fungal pathogen gibberella fujikuroi of the protein encoded by arabidopsis atelhyprp2 (earli1-like hybrid proline-rich protein 2, at4g12500) were investigated using transgenic tobacco plants. the coding sequence of atelhyprp2 was amplified from genomic dna of col-0 ecotype. after restriction digestion, the pcr fragment was ligated into pcambia1302 to produce a fusion expression vector, pcambia1302-atelhyprp2-gfp. then the recombinant plasmid was ... | 2014 | 25007583 |
| inhibitory and toxic effects of volatiles emitted by strains of pseudomonas and serratia on growth and survival of selected microorganisms, caenorhabditis elegans, and drosophila melanogaster. | in previous research, volatile organic compounds (vocs) emitted by various bacteria into the chemosphere were suggested to play a significant role in the antagonistic interactions between microorganisms occupying the same ecological niche and between bacteria and target eukaryotes. moreover, a number of volatiles released by bacteria were reported to suppress quorum-sensing cell-to-cell communication in bacteria, and to stimulate plant growth. here, volatiles produced by pseudomonas and serratia ... | 2014 | 25006575 |
| structure of d-tagatose 3-epimerase-like protein from methanocaldococcus jannaschii. | the crystal structure of a d-tagatose 3-epimerase-like protein (mj1311p) encoded by a hypothetical open reading frame, mj1311, in the genome of the hyperthermophilic archaeon methanocaldococcus jannaschii was determined at a resolution of 2.64 å. the asymmetric unit contained two homologous subunits, and the dimer was generated by twofold symmetry. the overall fold of the subunit proved to be similar to those of the d-tagatose 3-epimerase from pseudomonas cichorii and the d-psicose 3-epimerases ... | 2014 | 25005083 |
| riboregulation in plant-associated α-proteobacteria. | the symbiotic α-rhizobia sinorhizobium meliloti, bradyrhizobium japonicum, rhizobium etli and the related plant pathogen agrobacterium tumefaciens are important model organisms for studying plant-microbe interactions. these metabolically versatile soil bacteria are characterized by complex lifestyles and large genomes. here we summarize the recent knowledge on their small non-coding rnas (srnas) including conservation, function, and interaction of the srnas with the rna chaperone hfq. in each of ... | 2014 | 25003187 |
| rhizobial homologs of the fatty acid transporter fadl facilitate perception of long-chain acyl-homoserine lactone signals. | quorum sensing (qs) using n-acyl homoserine lactones (ahls) as signal molecules is a common strategy used by diverse gram-negative bacteria. a widespread mechanism of ahl sensing involves binding of these molecules by cytosolic luxr-type transcriptional regulators, which requires uptake of external ahls. the outer membrane is supposed to be an efficient barrier for diffusion of long-chain ahls. here we report evidence that in sinorhizobium meliloti, sensing of ahls with acyl chains composed of 1 ... | 2014 | 25002473 |
| ghh2a12, a replication-dependent histone h2a gene from gossypium hirsutum, is negatively involved in the development of cotton fiber cells. | ghh2a12 was preferentially expressed at the initiation and early elongation stage of cotton fiber development, and overexpression of ghh2a12 caused retardation of fiber initiation and produced shorter fibers. histone h2a is a component of eukaryotic chromatin whose function has not been studied in cotton. we have isolated an h2a gene encoding 156 amino acids, named ghh2a12. like other plant histone h2as, ghh2a12 contains a typical spkk motif in the carboxy-terminal and a plant-unique peptide-bin ... | 2014 | 25001001 |
| bidirectional promoter trapping t-dna for insertional mutagenesis in verticillium dahliae. | transfer dna (t-dna)-based random insertional mutagenesis is a universal forward genetic approach for gene identification and cloning in many phytopathogenic fungi. in a large number of randomly selected transformants, screening for mutants with a specific phenotype is laborious, especially for pathogenicity-defective mutants. to accelerate mutant screening and gene identification, a bidirectional promoter-trapping ti binary vector, 1300-bisgfp-hyg, was constructed and deployed in this study. mo ... | 2014 | 24993736 |
| agrobacterium tumefaciens-mediated genetic transformation of haptophytes (isochrysis species). | isochrysis galbana and isochrysis sp. are economically important microalgae from the division of haptophytes. here, we report agrobacterium-mediated stable dna transfer into their nuclear genomes. initial studies were performed to standardize co-cultivation media and determine the sensitivity of the microalgae to selective agents. up to 1 mg/ml of the antibiotic hygromycin did not inhibit growth, whereas both the haptophytes bleached in artificial seawater (asw) medium containing micromolar conc ... | 2014 | 24993358 |
| agrobacterium tumefaciens exor controls acid response genes and impacts exopolysaccharide synthesis, horizontal gene transfer, and virulence gene expression. | agrobacterium tumefaciens is a facultative plant pathogen and the causative agent of crown gall disease. the initial stage of infection involves attachment to plant tissues, and subsequently, biofilms may form at these sites. this study focuses on the periplasmic exor regulator, which was identified based on the severe biofilm deficiency of a. tumefaciens exor mutants. genome-wide expression analysis was performed to elucidate the complete exor regulon. overproduction of the exopolysaccharide su ... | 2014 | 24982308 |
| agrobacterium tumefaciens deploys a superfamily of type vi secretion dnase effectors as weapons for interbacterial competition in planta. | the type vi secretion system (t6ss) is a widespread molecular weapon deployed by many proteobacteria to target effectors/toxins into both eukaryotic and prokaryotic cells. we report that agrobacterium tumefaciens, a soil bacterium that triggers tumorigenesis in plants, produces a family of type vi dnase effectors (tde) that are distinct from previously known polymorphic toxins and nucleases. tde exhibits an antibacterial dnase activity that relies on a conserved hxxd motif and can be counteracte ... | 2014 | 24981331 |
| identification of essential alphaproteobacterial genes reveals operational variability in conserved developmental and cell cycle systems. | the cell cycle of caulobacter crescentus is controlled by a complex signalling network that co-ordinates events. genome sequencing has revealed many c. crescentus cell cycle genes are conserved in other alphaproteobacteria, but it is not clear to what extent their function is conserved. as many cell cycle regulatory genes are essential in c. crescentus, the essential genes of two alphaproteobacteria, agrobacterium tumefaciens (rhizobiales) and brevundimonas subvibrioides (caulobacterales), were ... | 2014 | 24975755 |
| expression and functional characterization of the agrobacterium virb2 amino acid substitution variants in t-pilus biogenesis, virulence, and transient transformation efficiency. | agrobacterium tumefaciens is a phytopathogenic bacterium that causes crown gall disease by transferring transferred dna (t-dna) into the plant genome. the translocation process is mediated by the type iv secretion system (t4ss) consisting of the vird4 coupling protein and 11 virb proteins (virb1 to virb11). all virb proteins are required for the production of t-pilus, which consists of processed virb2 (t-pilin) and virb5 as major and minor subunits, respectively. virb2 is an essential component ... | 2014 | 24971727 |
| gxysba abc transporter of agrobacterium tumefaciens and its role in sugar utilization and vir gene expression. | monosaccharides available in the extracellular milieu of agrobacterium tumefaciens can be transported into the cytoplasm, or via the periplasmic sugar binding protein, chve, play a critical role in controlling virulence gene expression. the chve-mmsab abc transporter is involved in the utilization of a wide range of monosaccharide substrates but redundant transporters are likely given the ability of a chve-mmsab deletion strain to grow, albeit more slowly, in the presence of particular monosacch ... | 2014 | 24957625 |
| ligand-binding pocket bridges dna-binding and dimerization domains of the urate-responsive marr homologue mftr from burkholderia thailandensis. | members of the multiple antibiotic resistance regulator (marr) family often regulate gene activity by responding to a specific ligand. in the absence of ligand, most marr proteins function as repressors, while ligand binding causes attenuated dna binding and therefore increased gene expression. previously, we have shown that urate is a ligand for mftr (major facilitator transport regulator), which is encoded by the soil bacterium burkholderia thailandensis. we show here that both mftr and the di ... | 2014 | 24955985 |
| integrative gene transfer in the truffle tuber borchii by agrobacterium tumefaciens-mediated transformation. | agrobacterium tumefaciens-mediated transformation is a powerful tool for reverse genetics and functional genomic analysis in a wide variety of plants and fungi. tuber spp. are ecologically important and gastronomically prized fungi ("truffles") with a cryptic life cycle, a subterranean habitat and a symbiotic, but also facultative saprophytic lifestyle. the genome of a representative member of this group of fungi has recently been sequenced. however, because of their poor genetic tractability, i ... | 2014 | 24949275 |
| xyloglucan endotransglucosylase-hydrolase17 interacts with xyloglucan endotransglucosylase-hydrolase31 to confer xyloglucan endotransglucosylase action and affect aluminum sensitivity in arabidopsis. | previously, we reported that although the arabidopsis (arabidopsis thaliana) xyloglucan endotransglucosylase-hydrolase31 (xth31) has predominately xyloglucan endohydrolase activity in vitro, loss of xth31 results in remarkably reduced in vivo xyloglucan endotransglucosylase (xet) action and enhanced al resistance. here, we report that xth17, predicted to have xet activity, binds xth31 in yeast (saccharomyces cerevisiae) two-hybrid and coimmunoprecipitations assays and that this interaction may b ... | 2014 | 24948835 |
| a putative soybean gmssos1 confers enhanced salt tolerance to transgenic arabidopsis sos1-1 mutant. | the cdna of gmssos1, a putative plasma membrane na(+)/h(+) antiporter gene isolated from glycine max, glycine soja, and their hybrid, was constructed into plant expression vector pcambia 1300 and then transformed with agrobacterium tumefaciens under the control of camv 35s promoter to arabidopsis thaliana wild-type (wt) and mutant (atsos1-1) plants. by hygromycin resistance detection and pcr analysis, transgenic plants (wt35s:gmssos1 and atsos1-1 35s:gmssos1) were obtained. seed germination, see ... | 2015 | 24934653 |
| an efficient agrobacterium-mediated transformation system for poplar. | poplar is a model system for the regeneration and genetic transformation of woody plants. to shorten the time required for studies of transgenic poplar, efforts have been made to optimize transformation methods that use agrobacterium tumefaciens. in this study, an agrobacterium infective suspension was treated at 4 °c for at least 10 h before infecting explants. by transforming the populus hybrid clone "nanlin895" (populus deltoides×p. euramericana) with agrobacterium harboring the pbi121:carnac ... | 2014 | 24933641 |
| a golden gate modular cloning toolbox for plants. | plant synthetic biology requires robust and efficient methods for assembling multigene constructs. golden gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. we present here a versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable golden gate construction of multigene constructs for plant transformation. parts include promoters, untranslated sequences, reporters, antigeni ... | 2014 | 24933124 |
| functional characterization of 5-enopyruvylshikimate-3-phosphate synthase from alkaliphilus metalliredigens in transgenic arabidopsis. | although a large number of aroa enzymes (epsps: 5-enopyruvylshikimate-3-phosphate synthase) have been identified, cloned, and tested for glyphosate resistance, only two aroa variants, derived from agrobacterium tumefaciens strain cp4 and zea mays, have been utilized to produce the commercial glyphosate-resistant crops. here, we have used a pcr-based twostep dna synthesis method to synthesize an aroa gene (aroaa. metalliredigens) from alkaliphilus metalliredigens, encoding a new epsps. furthermor ... | 2014 | 24931502 |
| enzymatic properties and substrate specificity of a bacterial phosphatidylcholine synthase. | phosphatidylcholine (pc) is a rare membrane lipid in bacteria, but is crucial for virulence of the plant pathogen agrobacterium tumefaciens and various other pathogens. agrobacterium tumefaciens uses two independent pc biosynthesis pathways. one is dependent on the integral membrane protein pc synthase (pcs), which catalyzes the conversion of cytidine diphosphate-diacylglycerol (cdp-dag) and choline to pc, thereby releasing a cytidine monophosphate (cmp). here, we show that pcs consists of eight ... | 2014 | 24931117 |
| construction of a standard reference plasmid containing seven target genes for the detection of transgenic cotton. | insect resistance and herbicide tolerance are the dominant traits of commercialized transgenic cotton. in this study, we constructed a general standard reference plasmid for transgenic cotton detection. target genes, including the cowpea trypsin gene cpti, the insect resistance gene cry1ab/1ac, the herbicide tolerance gene cp4-epsps, the agrobacterium tumefaciens nopaline synthase (nos) terminator that exists in transgenic cotton and part of the endogenous cotton sadi gene were amplified from pl ... | 2014 | 24929128 |
| evolution of enzymatic activities in the enolase superfamily: galactarate dehydratase iii from agrobacterium tumefaciens c58. | the genome of agrobacterium tumefaciens c58 encodes 12 members of the enolase superfamily (ens), eight of which are members of the mandelate racemase (mr) subgroup and, therefore, likely to be acid sugar dehydratases. using a library of 77 acid sugars for high-throughput screening, one protein (uniprot entry a9cg74; locus tag atu4196) showed activity with both m-galactarate and d-galacturonate. two families of galactarate dehydratases had been discovered previously in the ens, galrd/talrd [yew, ... | 2014 | 24926996 |
| attachment of agrobacterium to plant surfaces. | agrobacterium tumefaciens binds to the surfaces of inanimate objects, plants, and fungi. these bacteria are excellent colonizers of root surfaces. in addition, they also bind to soil particles and to the surface of artificial or man-made substances, such as polyesters and plastics. the mechanisms of attachment to these different surfaces have not been completely elucidated. at least two types of binding have been described unipolarpolysaccharide-dependent polar attachment and unipolar polysaccha ... | 2014 | 24926300 |
| stable expression of mtld gene imparts multiple stress tolerance in finger millet. | finger millet is susceptible to abiotic stresses, especially drought and salinity stress, in the field during seed germination and early stages of seedling development. therefore developing stress tolerant finger millet plants combating drought, salinity and associated oxidative stress in these two growth stages is important. cellular protection through osmotic adjustment and efficient free radical scavenging ability during abiotic stress are important components of stress tolerance mechanisms i ... | 2014 | 24922513 |
| two separate modules of the conserved regulatory rna abcr1 address multiple target mrnas in and outside of the translation initiation region. | the small rna abcr1 regulates the expression of abc transporters in the plant pathogen agrobacterium tumefaciens, the plant symbiont sinorhizobium meliloti, and the human pathogen brucella abortus. a combination of proteomic and bioinformatic approaches suggested dozens of abcr1 targets in a. tumefaciens. several of these newly discovered targets are involved in the uptake of amino acids, their derivatives, and sugars. among the latter is the periplasmic sugar-binding protein chve, a component o ... | 2014 | 24921646 |
| gene targeting using the agrobacterium tumefaciens-mediated crispr-cas system in rice. | the type ii clustered, regularly interspaced, short palindromic repeat (crispr)/ crispr-associated protein 9 (cas9) system is a novel molecular tool for site-specific genome modification. the crispr-cas9 system was recently introduced into plants by transient or stable transformation. | 2014 | 24920971 |
| an arabidopsis mutant impaired in intracellular calcium elevation is sensitive to biotic and abiotic stress. | ca2+, a versatile intracellular second messenger in various signaling pathways, initiates many responses involved in growth, defense and tolerance to biotic and abiotic stress. endogenous and exogenous signals induce cytoplasmic ca2+ ([ca2+]cyt) elevation, which are responsible for the appropriate downstream responses. | 2014 | 24920452 |
| gene-splitting technology: a novel approach for the containment of transgene flow in nicotiana tabacum. | the potential impact of transgene escape on the environment and food safety is a major concern to the scientists and public. this work aimed to assess the effect of intein-mediated gene splitting on containment of transgene flow. two fusion genes, epspsn-in and ic-epspsc, were constructed and integrated into n. tabacum, using agrobacterium tumefaciens-mediated transformation. epspsn-in encodes the first 295 aa of the herbicide resistance gene 5-enolpyruvyl shikimate-3-phosphate synthase (epsps) ... | 2014 | 24915192 |
| protozoan alkbh8 oxygenases display both dna repair and trna modification activities. | the alkbh family of fe(ii) and 2-oxoglutarate dependent oxygenases comprises enzymes that display sequence homology to alkb from e. coli, a dna repair enzyme that uses an oxidative mechanism to dealkylate methyl and etheno adducts on the nucleobases. humans have nine different alkbh proteins, alkbh1-8 and fto. mammalian and plant alkbh8 are trna hydroxylases targeting 5-methoxycarbonylmethyl-modified uridine (mcm5u) at the wobble position of trnagly(ucc). in contrast, the genomes of some bacteri ... | 2014 | 24914785 |
| optimization of genetic transformation of artemisia annua l. using agrobacterium for artemisinin production. | artemisinin, a sesquiterpene lactone endoperoxide isolated from the medicinal plant artemisia annua l., is a choice and effective drug for malaria treatment. due to the low yield of artemisinin in plants, there is a need to enhance the production of artemisinin from a. annua and biotechnological technique may be one of the methods that can be used for the purpose. | 2014 | 24914301 |
| the ctp type ivb pilus locus of agrobacterium tumefaciens directs formation of the common pili and contributes to reversible surface attachment. | agrobacterium tumefaciens can adhere to plant tissues and abiotic surfaces and forms biofilms. cell surface appendages called pili play an important role in adhesion and biofilm formation in diverse bacterial systems. the a. tumefaciens c58 genome sequence revealed the presence of the ctpabcdefghi genes (cluster of type iv pili; atu0216 to atu0224), homologous to tad-type pilus systems from several bacteria, including aggregatibacter actinomycetemcomitans and caulobacter crescentus. these system ... | 2014 | 24914181 |
| mutational analysis of the ve1 immune receptor that mediates verticillium resistance in tomato. | pathogenic verticillium species are economically important plant pathogens that cause vascular wilt diseases in hundreds of plant species. the ve1 gene of tomato confers resistance against race 1 strains of verticillium dahliae and v. albo-atrum. ve1 encodes an extracellular leucine-rich repeat (elrr) receptor-like protein (rlp) that serves as a cell surface receptor for recognition of the recently identified secreted verticillium effector ave1. to investigate recognition of ave1 by ve1, alanine ... | 2014 | 24911915 |
| construction and functional analysis of trichoderma harzianum mutants that modulate maize resistance to the pathogen curvularia lunata. | agrobacterium tumefaciens-mediated transformation (atmt) was used to generate an insertional mutant library of the mycelial fungus trichoderma harzianum. from a total of 450 mutants, six mutants that showed significant influence on maize resistance to c. lunata were analyzed in detail. maize coated with these mutants was more susceptible to c. lunata compared with those coated with a wild-type (wt) strain. similar to other fungal atmt libraries, all six mutants were single copy integrations, whi ... | 2014 | 24901960 |
| psim24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants. | we have constructed a small and highly efficient binary ti vector psim24 for plant transformation with maximum efficacy. in the psim24 vector, the size of the backbone of the early binary vector pkylxm24 (genbank accession no. hm036220; a derivative of pkylx71) was reduced from 12.8 kb to 7.1 kb. the binary vector psim24 is composed of the following genetic elements: left and right t-dna borders, a modified full-length transcript promoter (m24) of mirabilis mosaic virus with duplicated enhancer ... | 2014 | 24897541 |
| functional characterization of pmt2, encoding a protein-o-mannosyltransferase, in the human pathogen cryptococcus neoformans. | diazobenzoic acid b (dbb), also known as diazonium blue b or fast blue b, can be used to distinguish basidiomycetous yeasts from ascomycetes. this chemical has long been used for the taxonomic study of yeast species at the phylum level, but the mechanism underlying the dbb staining remains unknown. to identify molecular targets of dbb staining, we isolated agrobacterium tumefaciens-mediated insertional mutants of cryptococcus neoformans, a basidiomycetous pathogenic yeast, which were negative to ... | 2014 | 24892554 |
| agrobacterium-derived cytokinin influences plastid morphology and starch accumulation in nicotiana benthamiana during transient assays. | agrobacterium tumefaciens-based transient assays have become a common tool for answering questions related to protein localization and gene expression in a cellular context. the use of these assays assumes that the transiently transformed cells are observed under relatively authentic physiological conditions and maintain 'normal' sub-cellular behaviour. although this premise is widely accepted, the question of whether cellular organization and organelle morphology is altered in agrobacterium-inf ... | 2014 | 24886417 |
| hairy root transformation using agrobacterium rhizogenes as a tool for exploring cell type-specific gene expression and function using tomato as a model. | agrobacterium rhizogenes (or rhizobium rhizogenes) is able to transform plant genomes and induce the production of hairy roots. we describe the use of a. rhizogenes in tomato (solanum spp.) to rapidly assess gene expression and function. gene expression of reporters is indistinguishable in plants transformed by agrobacterium tumefaciens as compared with a. rhizogenes. a root cell type- and tissue-specific promoter resource has been generated for domesticated and wild tomato (solanum lycopersicum ... | 2014 | 24868032 |
| peptidoglycan synthesis machinery in agrobacterium tumefaciens during unipolar growth and cell division. | the synthesis of peptidoglycan (pg) in bacteria is a crucial process controlling cell shape and vitality. in contrast to bacteria such as escherichia coli that grow by dispersed lateral insertion of pg, little is known of the processes that direct polar pg synthesis in other bacteria such as the rhizobiales. to better understand polar growth in the rhizobiales agrobacterium tumefaciens, we first surveyed its genome to identify homologs of (~70) well-known pg synthesis components. since most of t ... | 2014 | 24865559 |
| role of the vira histidine autokinase of agrobacterium tumefaciens in the initial steps of pathogenesis. | histidine kinases serve as critical environmental sensing modules, and despite their designation as simple two-component modules, their functional roles are remarkably diverse. in agrobacterium tumefaciens pathogenesis, vira serves with virg as the initiating sensor/transcriptional activator for inter-kingdom gene transfer and transformation of higher plants. through responses to three separate signal inputs, low ph, sugars, and phenols, a. tumefaciens commits to pathogenesis in virtually all fl ... | 2014 | 24860585 |
| mechanisms and regulation of surface interactions and biofilm formation in agrobacterium. | for many pathogenic bacteria surface attachment is a required first step during host interactions. attachment can proceed to invasion of host tissue or cells or to establishment of a multicellular bacterial community known as a biofilm. the transition from a unicellular, often motile, state to a sessile, multicellular, biofilm-associated state is one of the most important developmental decisions for bacteria. agrobacterium tumefaciens genetically transforms plant cells by transfer and integratio ... | 2014 | 24834068 |
| hemoglobin uptake by paracoccidioides spp. is receptor-mediated. | iron is essential for the proliferation of fungal pathogens during infection. the availability of iron is limited due to its association with host proteins. fungal pathogens have evolved different mechanisms to acquire iron from host; however, little is known regarding how paracoccidioides species incorporate and metabolize this ion. in this work, host iron sources that are used by paracoccidioides spp. were investigated. robust fungal growth in the presence of the iron-containing molecules hemi ... | 2014 | 24831516 |
| agrobacterium-mediated transformation of tomato with rolb gene results in enhancement of fruit quality and foliar resistance against fungal pathogens. | tomato (solanum lycopersicum l.) is the second most important cultivated crop next to potato, worldwide. tomato serves as an important source of antioxidants in human diet. alternaria solani and fusarium oxysporum cause early blight and vascular wilt of tomato, respectively, resulting in severe crop losses. the foremost objective of the present study was to generate transgenic tomato plants with rolb gene and evaluate its effect on plant morphology, nutritional contents, yield and resistance aga ... | 2014 | 24817272 |
| plant responses to agrobacterium tumefaciens and crown gall development. | agrobacterium tumefaciens causes crown gall disease on various plant species by introducing its t-dna into the genome. therefore, agrobacterium has been extensively studied both as a pathogen and an important biotechnological tool. the infection process involves the transfer of t-dna and virulence proteins into the plant cell. at that time the gene expression patterns of host plants differ depending on the agrobacterium strain, plant species and cell-type used. later on, integration of the t-dna ... | 2014 | 24795740 |
| high-level soluble expression of the hema gene from rhodobacter capsulatus and comparative study of its enzymatic properties. | the rhodobacter capsulatus hema gene, which encodes 5-aminolevulinic acid synthase (alas), was expressed in escherichia coli rosetta (de3) and the enzymatic properties of the purified recombinant alas (rc-alas) were studied. compared with alass encoded by hema genes from agrobacterium radiobacter (ar-alas) and rhodobacter sphaeroides (rs-alas), the specific activity of rc-alas reached 198.2 u/mg, which was about 31.2% and 69.5% higher than those of ar-alas (151.1 u/mg) and rs-alas (116.9 u/mg), ... | 2014 | 24793767 |
| immobilized synthetic pathway for biodegradation of toxic recalcitrant pollutant 1,2,3-trichloropropane. | the anthropogenic compound 1,2,3-trichloropropane (tcp) has recently drawn attention as an emerging groundwater contaminant. no living organism, natural or engineered, is capable of the efficient aerobic utilization of this toxic industrial waste product. we describe a novel biotechnology for transforming tcp based on an immobilized synthetic pathway. the pathway is composed of three enzymes from two different microorganisms: engineered haloalkane dehalogenase from rhodococcus rhodochrous ncimb ... | 2014 | 24787668 |
| a betasatellite-dependent begomovirus infects ornamental rose: characterization of begomovirus infecting rose in pakistan. | the begomovirus genus of the family geminiviridae comprises the largest group of geminiviruses. the list of begomoviruses is continuously increasing as a result of improvement in the methods for identification. ornamental rose plants (rosa chinensis) with highly stunted growth and leaf curling were found in faisalabad, pakistan. plants were analyzed for begomovirus infection, through rolling circle amplification and pcr methods. based on complete genome sequence homologies with other begomovirus ... | 2014 | 24781196 |
| expression, characterization, and improvement of a newly cloned halohydrin dehalogenase from agrobacterium tumefaciens and its application in production of epichlorohydrin. | a gene encoding halohydrin dehalogenase (hhdh) from agrobacterium tumefaciens cctcc m 87071 was cloned and expressed in escherichia coli. to increase activity and stability of hhdh, 14 amino acid residues around the active site and substrate-binding pocket based on the structural analysis and molecular docking were selected as targets for site-directed mutagenesis. the studies showed that the mutant hhdh (mut-hhdh) enzyme had a more accessible substrate-binding pocket than the wild-type hhdh (wt ... | 2014 | 24777710 |
| thc6 protein, isolated from trichoderma harzianum, can induce maize defense response against curvularia lunata. | mutant t66 was isolated from 450 mutants (constructed with agrobacterium tumefaciens-mediated transformation method) of trichoderma harzianum. maize seeds coated with t66 were more susceptible to curvularia lunata when compared with those coated with wild-type (wt) strain. the disease index of maize treated with t66 and wt were 62.5 and 42.1%, respectively. further research showed t-dna has inserted into the orf of one gene, which resulted in the functional difference between wt and t66. the gen ... | 2015 | 24771614 |
| crystal structure of the novel haloalkane dehalogenase data from agrobacterium tumefaciens c58 reveals a special halide-stabilizing pair and enantioselectivity mechanism. | a novel haloalkane dehalogenase data from agrobacterium tumefaciens c58 belongs to the hld-ii subfamily and hydrolyzes brominated and iodinated compounds, leading to the generation of the corresponding alcohol, a halide ion, and a proton. because data possesses a unique asn-tyr pair instead of the asn-trp pair conserved among the subfamily members, which was proposed to keep the released halide ion stable, the structural basis for its reaction mechanism should be elucidated. here, we determined ... | 2014 | 24770384 |
| identification of a stop1-like protein in eucalyptus that regulates transcription of al tolerance genes. | tolerance to soil acidity is an important trait for eucalyptus clones that are introduced to commercial forestry plantations in pacific asian countries, where acidic soil is dominant in many locations. a conserved transcription factor regulating aluminum (al) and proton (h⁺) tolerance in land-plant species, stop1 (sensitive toproton rhizotoxicity 1)-like protein, was isolated by polymerase chain reaction-based cloning, and then suppressed by rna interference in hairy roots produced by agrobacter ... | 2014 | 24767110 |
| draft genome sequence of umbilicaria muehlenbergii kolrilf000956, a lichen-forming fungus amenable to genetic manipulation. | umbilicaria muehlenbergii strain kolrilf000956 is amenable to agrobacterium tumefaciens-mediated transformation (atmt), making it the only known genetically tractable lichen-forming fungus to date. we report another advancement in lichen genetics, a draft genome assembly for u. muehlenbergii with a size of 34,812,353 bp and a gc content of 47.12%, consisting of seven scaffolds. | 2014 | 24762942 |
| the detection of t-nos, a genetic element present in gmos, by cross-priming isothermal amplification with real-time fluorescence. | an isothermal cross-priming amplification (cpa) assay for agrobacterium tumefaciens nopaline synthase terminator (t-nos) was established and investigated in this work. a set of six specific primers, recognizing eight distinct regions on the t-nos sequence, was designed. the cpa assay was performed at a constant temperature, 63 °c, and detected by real-time fluorescence. the results indicated that real-time fluorescent cpa had high specificity, and the limit of detection was 1.06 × 10(3) copies o ... | 2014 | 24748469 |
| identification of effective pb resistant bacteria isolated from lens culinaris growing in lead contaminated soils. | soil bacteria are a new phytoremediation system for the removal of heavy metals from soils. in this study, fifteen soil bacteria were isolated from root nodules of lentil growing in heavy metals contaminated soils, particularly by lead. molecular characterization of the collection showed a large diversity, including agrobacterium tumefaciens, rahnella aquatilis, pseudomonas, and rhizobium sp. these soil bacteria had a wide range of tolerance to heavy metals. among them, strains of a. tumefaciens ... | 2015 | 24740715 |
| isolation of the autoinducer-quenching strain that inhibits lasr in pseudomonas aeruginosa. | quorum sensing (qs) has been recognized as a general phenomenon in microorganisms and plays an important role in many pathogenic bacteria. in this report, we used the agrobacterium tumefaciens biosensor strain nt1 to rapidly screen for autoinducer-quenching inhibitors from bacteria. after initial screening 5389 isolates obtained from land and beach soil, 53 putative positive strains were identified. a confirmatory bioassay was carried out after concentrating the putative positive culture superna ... | 2014 | 24736783 |
| the myosin motor domain-containing chitin synthase pdchsvii is required for development, cell wall integrity and virulence in the citrus postharvest pathogen penicillium digitatum. | chitin is an essential component of the fungal cell wall and a potential target in the development of new antifungal compounds, due to its presence in fungi and not in plants or vertebrates. chitin synthase genes (chs) constitute a complex family in filamentous fungi and are involved in fungal development, morphogenesis, pathogenesis and virulence. in this study, additional chs genes in the citrus postharvest pathogen penicillium digitatum have been identified. comparative analyses included each ... | 2014 | 24727399 |
| production of proteasome inhibitor syringolin a by the endophyte rhizobium sp. strain ap16. | syringolin a, the product of a mixed nonribosomal peptide synthetase/polyketide synthase encoded by the syl gene cluster, is a virulence factor secreted by certain pseudomonas syringae strains. together with the glidobactins produced by a number of beta- and gammaproteobacterial human and animal pathogens, it belongs to the syrbactins, a structurally novel class of proteasome inhibitors. in plants, proteasome inhibition by syringolin a-producing p. syringae strains leads to the suppression of ho ... | 2014 | 24727275 |
| membrane lipids in agrobacterium tumefaciens: biosynthetic pathways and importance for pathogenesis. | many cellular processes critically depend on the membrane composition. in this review, we focus on the biosynthesis and physiological roles of membrane lipids in the plant pathogen agrobacterium tumefaciens. the major components of a. tumefaciens membranes are the phospholipids (pls), phosphatidylethanolamine (pe), phosphatidylglycerol, phosphatidylcholine (pc) and cardiolipin, and ornithine lipids (ols). under phosphate-limited conditions, the membrane composition shifts to phosphate-free lipid ... | 2014 | 24723930 |