Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| bacterial metabolism shapes the host-pathogen interface. | bacterial pathogens have evolved to exploit humans as a rich source of nutrients to support survival and replication. the pathways of bacterial metabolism that permit successful colonization are surprisingly varied and highlight remarkable metabolic flexibility. the constraints and immune pressures of distinct niches within the human body set the stage for understanding the mechanisms by which bacteria acquire critical nutrients. in this article we discuss how different bacterial pathogens carry ... | 2016 | 27337445 |
| molecular evolution of the oxygen-binding hemerythrin domain. | the evolution of oxygenic photosynthesis during precambrian times entailed the diversification of strategies minimizing reactive oxygen species-associated damage. four families of oxygen-carrier proteins (hemoglobin, hemerythrin and the two non-homologous families of arthropodan and molluscan hemocyanins) are known to have evolved independently the capacity to bind oxygen reversibly, providing cells with strategies to cope with the evolutionary pressure of oxygen accumulation. oxygen-binding hem ... | 2016 | 27336621 |
| optimization of quantitative pcr methods for enteropathogen detection. | detection and quantification of enteropathogens in stool specimens is useful for diagnosing the cause of diarrhea but is technically challenging. here we evaluate several important determinants of quantification: specimen collection, nucleic acid extraction, and extraction and amplification efficiency. first, we evaluate the molecular detection and quantification of pathogens in rectal swabs versus stool, using paired flocked rectal swabs and whole stool collected from 129 children hospitalized ... | 2016 | 27336160 |
| risk factors associated with campylobacter detected by pcr in humans and animals in rural cambodia. | campylobacter are worldwide-occurring zoonotic bacteria, with the species campylobacter jejuni and c. coli commonly associated with diarrhoea in children in low-income countries. in this cross-sectional study, the prevalence of c. jejuni and c. coli in human and livestock faecal samples was detected by pcr and zoonotic risk factors associated with human campylobacter positivity were identified. in total 681 humans and 753 livestock (chickens, ducks, pigs, cattle) from 269 households were sampled ... | 2016 | 27334412 |
| monitoring antimicrobial resistance in the food supply chain and its implications for fda policy initiatives. | in response to concerning increases in antimicrobial resistance (amr), the food and drug administration (fda) has decided to increase veterinary oversight requirements for antimicrobials and restrict their use in growth promotion. given the high stakes of this policy for the food supply, economy, and human and veterinary health, it is important to rigorously assess the effects of this policy. we have undertaken a detailed analysis of data provided by the national antimicrobial resistance monitor ... | 2016 | 27324772 |
| interleukin-18 mediates immune responses to campylobacter jejuni infection in gnotobiotic mice. | human campylobacter jejuni infections are progressively rising worldwide. information about the molecular mechanisms underlying campylobacteriosis, however, are limited. in the present study we investigated whether cytokines such as il-23, il-22 and il-18, which share pivotal functions in host immunity, were involved in mediating intestinal and systemic immunopathological responses upon c. jejuni infection. | 2016 | 27322540 |
| gelc-ms-based proteomics of chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide. | chromobacterium violaceum is a free-living bacillus with several genes that enables it survival under different harsh environments such as oxidative and temperature stresses. here we performed a label-free quantitative proteomic study to unravel the molecular mechanisms that enable c. violaceum to survive oxidative stress. to achieve this, total proteins extracted from control and c. violaceum cultures exposed during two hours with 8 mm hydrogen peroxide were analyzed using gelc-ms proteomics. a ... | 2016 | 27321545 |
| campylobacter bacteraemia: 16 years of experience in a single centre. | campylobacter bacteraemia (cb) is rare and usually occurs in immune-compromised patients. in this study we examined the incidence and epidemiology of cb in one institution over 15.5 years. | 2017 | 27320494 |
| detection of hepatitis e virus and other livestock-related pathogens in iowa streams. | manure application is a source of pathogens to the environment. through overland runoff and tile drainage, zoonotic pathogens can contaminate surface water and streambed sediment and could affect both wildlife and human health. this study examined the environmental occurrence of gene markers for livestock-related bacterial, protozoan, and viral pathogens and antibiotic resistance in surface waters within the south fork iowa river basin before and after periods of swine manure application on agri ... | 2016 | 27318519 |
| variation in the limit-of-detection of the prospect campylobacter microplate enzyme immunoassay in stools spiked with emerging campylobacter species. | campylobacter enteritis in humans is primarily associated with c. jejuni/coli infection. the impact of other campylobacter spp. is likely to be underestimated due to the bias of culture methods towards campylobacter jejuni/coli diagnosis. stool antigen tests are becoming increasingly popular and appear generally less species-specific. a review of independent studies of the prospect® campylobacter microplate enzyme immunoassay (eia) developed for c. jejuni/coli showed comparable diagnostic result ... | 2016 | 27317896 |
| restaurant cooking trends and increased risk for campylobacter infection. | in the united kingdom, outbreaks of campylobacter infection are increasingly attributed to undercooked chicken livers, yet many recipes, including those of top chefs, advocate short cooking times and serving livers pink. during 2015, we studied preferences of chefs and the public in the united kingdom and investigated the link between liver rareness and survival of campylobacter. we used photographs to assess chefs' ability to identify chicken livers meeting safe cooking guidelines. to investiga ... | 2016 | 27314748 |
| two linked enteroinvasive escherichia coli outbreaks, nottingham, uk, june 2014. | enteroinvasive escherichia coli (eiec) outbreaks are uncommon in europe. in june 2014, two eiec outbreaks occurred in nottingham, uk, within 2 days; outbreak a was linked to a takeaway restaurant and outbreak b to a wedding party. we conducted 2 analytical studies: a case-control study for outbreak a and a cohort study for outbreak b. we tested microbiological and environmental samples, including by using whole-genome sequencing. for both outbreaks combined, we identified 157 probable case-patie ... | 2016 | 27314432 |
| pathogen presence in european starlings inhabiting commercial piggeries in south australia. | the majority of bacterial diarrhea-causing illnesses in domestic pigs result from infection with escherichia coli, salmonella spp., or campylobacter spp. these bacterial enteropathogens also correspond with the most-common bacteria isolated from wild birds. additionally, viral pathogens such as avian influenza virus (aiv), west nile virus (wnv, including kunjin disease), and newcastle disease virus (ndv) may also be carried and transmitted by birds in australia. introduced european starlings (st ... | 2016 | 27309283 |
| evaluation of a multiplex real-time pcr assay for detecting major bacterial enteric pathogens in fecal specimens: intestinal inflammation and bacterial load are correlated in campylobacter infections. | a total of 1,056 native or cary-blair-preserved stool specimens were simultaneously tested by conventional stool culturing and by enteric bacterial panel (ebp) multiplex real-time pcr for campylobacter jejuni, campylobacter coli, salmonella spp., and shigellosis disease-causing agents (shigella spp. and enteroinvasive escherichia coli [eiec]). overall, 143 (13.5%) specimens tested positive by pcr for the targets named above; 3 coinfections and 109 (10.4%) campylobacter spp., 17 (1.6%) salmonella ... | 2016 | 27307458 |
| a large outbreak of campylobacter jejuni infection in a university college caused by chicken liver pâté, australia, 2013. | in october 2013, public health authorities were notified of a suspected outbreak of gastroenteritis in students and guests following a catered function at a university residential college. a retrospective cohort study was undertaken to examine whether foods served at the function caused illness. a total of 56 cases of gastroenteritis, including seven laboratory-confirmed cases of campylobacter jejuni infection, were identified in 235 eligible respondents. univariate analysis showed a significant ... | 2016 | 27306097 |
| [multilocus sequence typing analysis of 47 campylobacter jejuni strains isolated from poultry in hubei province]. | to study the epidemiological and molecular characteristics of campylobacter jejuni in poultry in hubei province, we used multilocus sequence typing method to classify 47 local c. jejuni strains. | 2016 | 27305789 |
| novel immunomodulatory flagellin-like protein flac in campylobacter jejuni and other campylobacterales. | the human diarrheal pathogens campylobacter jejuni and campylobacter coli interfere with host innate immune signaling by different means, and their flagellins, flaa and flab, have a low intrinsic property to activate the innate immune receptor toll-like receptor 5 (tlr5). we have investigated here the hypothesis that the unusual secreted, flagellin-like molecule flac present in c. jejuni, c. coli, and other campylobacterales might activate cells via tlr5 and interact with tlr5. flac shows striki ... | 2015 | 27303676 |
| novel immunomodulatory flagellin-like protein flac in campylobacter jejuni and other campylobacterales. | the human diarrheal pathogens campylobacter jejuni and campylobacter coli interfere with host innate immune signaling by different means, and their flagellins, flaa and flab, have a low intrinsic property to activate the innate immune receptor toll-like receptor 5 (tlr5). we have investigated here the hypothesis that the unusual secreted, flagellin-like molecule flac present in c. jejuni, c. coli, and other campylobacterales might activate cells via tlr5 and interact with tlr5. flac shows striki ... | 2015 | 27303676 |
| a designed experiments approach to optimizing maldi-tof ms spectrum processing parameters enhances detection of antibiotic resistance in campylobacter jejuni. | maldi-tof ms has been utilized as a reliable and rapid tool for microbial fingerprinting at the genus and species levels. recently, there has been keen interest in using maldi-tof ms beyond the genus and species levels to rapidly identify antibiotic resistant strains of bacteria. the purpose of this study was to enhance strain level resolution for campylobacter jejuni through the optimization of spectrum processing parameters using a series of designed experiments. a collection of 172 strains of ... | 2016 | 27303397 |
| anti-mrsa activities of enterocins dd28 and dd93 and evidences on their role in the inhibition of biofilm formation. | methicillin-resistant staphylococcus aureus (mrsa) has become a worrisome superbug. this work aimed at studying the effects of two class iib bacteriocins, enterocins dd28 and dd93 as anti-mrsa agents. thus, these bacteriocins were purified, from the cultures supernatants of enterococcus faecalis 28 and 93, using a simplified purification procedure consisting in a cation exchange chromatography and a reversed-phase high-performance liquid chromatography. the anti-staphylococcal activity was shown ... | 2016 | 27303396 |
| recent advances in screening of anti-campylobacter activity in probiotics for use in poultry. | campylobacteriosis is the most common cause of bacterial gastroenteritis worldwide. campylobacter species involved in this infection usually include the thermotolerant species campylobacter jejuni. the major reservoir for c. jejuni leading to human infections is commercial broiler chickens. poultry flocks are frequently colonized by c. jejuni without any apparent symptoms. risk assessment analyses have identified the handling and consumption of poultry meat as one of the most important sources o ... | 2016 | 27303366 |
| olive leaf extract from sicilian cultivar reduced lipid accumulation by inducing thermogenic pathway during adipogenesis. | olive leaves contain a wide variety of phenolic compounds belonging to phenolic acids, phenolic alcohols, flavonoids, and secoiridoids, and include also many other pharmacological active compounds. they could play an important role in human diet and health because of their ability to lower blood pressure, increase coronary arteries blood flow and decrease the risk of cardiovascular diseases. the aim of this study was to investigate the effect of olive leaf extract (ole) from sicilian cultivar on ... | 2016 | 27303302 |
| chicken cecal micrornas in the response to campylobacter jejuni inoculation by solexa sequencing. | campylobacter jejuni (c. jejuni) is one of major foodborne pathogen that cause human diarrhea by consuming c. jejuni contaminated chicken products. micrornas play an integral role in many different biological processes including bacteria and virus inoculation in chickens. in this study, we identified chicken mirnas responding to c. jejuni inoculation through solexa sequencing in the cecum. as a result, four mirnas were significantly differentially expressed between inoculated and non-inoculated ... | 2016 | 27303046 |
| a nationwide survey of pathogenic leptospires in urine of cattle and buffaloes by loop-mediated isothermal amplification (lamp) method in thailand, 2011-2013. | leptospirosis is a worldwide distributed zoonosis which has long been endemic in thailand. cattle and buffaloes are important livestock species that live in close contact with humans, especially in rural areas. these animals may, therefore, act as long-term carriers of leptospirosis for humans and other livestock species. the present study employed loop-mediated isothermal amplification (lamp) method to detect pathogenic leptospiral 16s rdna in the urine of cattle and buffaloes for assessing ass ... | 2016 | 27302016 |
| acute idiopathic polyradiculoneuritis concurrent with acquired myasthenia gravis in a west highland white terrier dog. | acute idiopathic polyradiculoneuritis, an animal model for the axonal form of the guillain - barre syndrome in humans and the acquired myasthenia gravis are different autoimmune disorders affecting the peripheral nerves and the neuromuscular junction, respectively. both lead to muscle weakness and possible respiratory failure. the coexistence of these two entities combined in the same patient is rare in humans and, to our knowledge, the present case is the first reported in dogs. | 2016 | 27301259 |
| resistance of campylobacter jejuni isolated from layer farms in northern jordan using microbroth dilution and disc diffusion techniques. | campylobacter jejuni is an important pathogen of significant public health importance. this pathogen is associated with human infection and has been isolated from mammals and birds. ninety-two cloacal c. jejuni isolates were identified from 35 layer farms in northern jordan. antimicrobial susceptibility was determined using minimal inhibitory concentration (mic) and disc diffusion techniques with variable suggested breakpoints. using mic and eucast cut-off values, the study revealed a significan ... | 2016 | 27300500 |
| visceral hypersensitive rats share common dysbiosis features with irritable bowel syndrome patients. | to evaluate gut microbial dysbiosis in two visceral hypersensitive models in comparison with irritable bowel syndrome (ibs) patients and to explore the extent to which these models capture the dysbiosis of ibs patients. | 2016 | 27298564 |
| campylobacter jejuni-associated perimyocarditis: two case reports and review of the literature. | campylobacter spp. are among the most common bacterial causes of gastroenteritis world-wide and mostly follow a benign course. we report two cases of campylobacter jejuni-associated perimyocarditis, the first two simultaneous cases published to date and the third and fourth cases over all in sweden, and a review of the literature. | 2016 | 27297408 |
| clinical features, therapeutic interventions and long-term aspects of hemolytic-uremic syndrome in norwegian children: a nationwide retrospective study from 1999-2008. | hemolytic-uremic syndrome (hus) is a clinical triad of microangiopathic hemolytic anemia, impaired renal function and thrombocytopenia, primarily affecting pre-school-aged children. hus can be classified into diarrhea-associated hus (d(+)hus), usually caused by shiga toxin-producing escherichia coli (stec), and non-diarrhea-associated hus (d(-)hus), both with potentially serious acute and long-term complications. few data exists on the clinical features and long-term outcome of hus in norway. th ... | 2016 | 27297224 |
| survival and risk comparison of campylobacter jejuni on various processed meat products. | the objective of this study was to investigate survival kinetics of campylobacter jejuni on various processed meat products (dry-cured ham, round ham with/without sodium nitrite, garlic seasoned ham with/without sodium nitrite, and sausage without sodium nitrite). additionally, a semi-quantitative risk assessment of c. jejuni on various processed meat products was conducted using fda-irisk 1.0. inoculated processed meat products with 6.0 ± 0.5 log cfu/g of c. jejuni were vacuum packed and stored ... | 2016 | 27294947 |
| recurrent diplopia in a pediatric patient with bickerstaff brainstem encephalitis. | introduction. acute complete external ophthalmoplegia is a rare finding in clinical practice that is associated with diseases affecting the neuromuscular junction, the oculomotor nerves, or the brainstem. ophthalmoplegia has been reported with acute ataxia in miller fisher syndrome (mfs) and bickerstaff brainstem encephalitis (bbe). up to 95% of these cases are associated with anti-gq1b antibodies. only a small number of cases of anti-gq1b negative mfs have been documented in pediatric patients. ... | 2016 | 27293928 |
| identification and functional analysis of two toxin-antitoxin systems in campylobacter jejuni. | toxin-antitoxin (ta) systems are widely distributed in bacteria and play an important role in maintaining plasmid stability. the leading foodborne pathogen, campylobacter jejuni, can carry multiple plasmids associated with antibiotic resistance or virulence. previously a virulence plasmid named pvir was identified in c. jejuni 81-176 and ia3902, but determining the role of pvir in pathogenesis has been hampered because the plasmid cannot be cured. in this study, we report the identification of t ... | 2016 | 27291507 |
| cellulases and beyond: the first 70 years of the enzyme producer trichoderma reesei. | more than 70 years ago, the filamentous ascomycete trichoderma reesei was isolated on the solomon islands due to its ability to degrade and thrive on cellulose containing fabrics. this trait that relies on its secreted cellulases is nowadays exploited by several industries. most prominently in biorefineries which use t. reesei enzymes to saccharify lignocellulose from renewable plant biomass in order to produce biobased fuels and chemicals. in this review we summarize important milestones of the ... | 2016 | 27287427 |
| acute tuberculous myopericarditis mimicking acute myocardial infarction: a case report and literature review. | a number of cases of acute myopericarditis mimicking acute myocardial infarction (ami) have previously been reported in the literature. however, to the best of our knowledge, such a case resulting from mycobacterium tuberculosis infection has not previously been described. the present study reports the case of a 21-year-old male patient presenting with acute chest pain, in whom focal st-segment elevation and elevated cardiac enzymes mimicked a diagnosis of ami. however, acute tuberculous myoperi ... | 2016 | 27284323 |
| how to move an amphipathic molecule across a lipid bilayer: different mechanisms for different abc transporters? | import of β-oxidation substrates into peroxisomes is mediated by atp binding cassette (abc) transporters belonging to subfamily d. in order to enter the β-oxidation pathway, fatty acids are activated by conversion to fatty acyl-coa esters, a reaction which is catalysed by acyl-coa synthetases (acss). here, we present evidence for an unusual transport mechanism, in which fatty acyl-coa substrates are accepted by abc subclass d protein (abcd) transporters, cleaved by the transporters during transi ... | 2016 | 27284041 |
| establishing virulence associated polyphosphate kinase 2 as a drug target for mycobacterium tuberculosis. | inorganic polyphosphate (polyp) plays an essential role in microbial stress adaptation, virulence and drug tolerance. the genome of mycobacterium tuberculosis encodes for two polyphosphate kinases (ppk-1, rv2984 and ppk-2, rv3232c) and polyphosphatases (ppx-1, rv0496 and ppx-2, rv1026) for maintenance of intracellular polyp levels. microbial polyphosphate kinases constitute a molecular mechanism, whereby microorganisms utilize polyp as phosphate donor for synthesis of atp. in the present study w ... | 2016 | 27279366 |
| environmental breviatea harbour mutualistic arcobacter epibionts. | breviatea form a lineage of free living, unicellular protists, distantly related to animals and fungi. this lineage emerged almost one billion years ago, when the oceanic oxygen content was low, and extant breviatea have evolved or retained an anaerobic lifestyle. here we report the cultivation of lenisia limosa, gen. et sp. nov., a newly discovered breviate colonized by relatives of animal-associated arcobacter. physiological experiments show that the association of l. limosa with arcobacter is ... | 2016 | 27279223 |
| a randomized clinical trial evaluating the effects of oligosaccharides on transfer of passive immunity in neonatal dairy calves. | bacterial contamination of colostrum is common and can decrease igg absorption in neonatal calves. strategies that mitigate this situation without complicating colostrum management will benefit dairy calf health and survival. | 2016 | 27278714 |
| a role for the intestinal microbiota and virome in myalgic encephalomyelitis/chronic fatigue syndrome (me/cfs)? | myalgic encephalomyelitis/chronic fatigue syndrome (me/cfs) is a heterogeneous disorder of significant societal impact that is proposed to involve both host and environmentally derived aetiologies that may be autoimmune in nature. immune-related symptoms of at least moderate severity persisting for prolonged periods of time are common in me/cfs patients and b cell depletion therapy is of significant therapeutic benefit. the origin of these symptoms and whether it is infectious or inflammatory in ... | 2016 | 27275835 |
| diagnosis of genus helicobacter through a hemi-nested pcr assay of 16s rrna. | the present study aimed to establish a genus-specific pcr-based assay to detect helicobacters using 16s rrna gene as the target template. we designed the hemi-nested primers based on sequences of 16s rrna gene of 34 types of helicobacter species. the inclusivity, sensitivity, and specificity of the pcr assay using these primers were examined in three different models, comprising feces simulated samples, blab/c mice infection model and clinic patients samples. the detection sensitivity of helicob ... | 2016 | 27275113 |
| calcium binding protects e-cadherin from cleavage by helicobacter pylori htra. | the cell adhesion and tumor suppressor protein e-cadherin is an important factor in the establishment and maintenance of epithelial integrity. e-cadherin is a single transmembrane protein, which consists of an intracellular domain (ic), a transmembrane domain (td), and five extracellular domains (ec). ec domains form homophilic interactions in cis and trans that require calcium binding to the linker region between the ec domains. in our previous studies, we identified the serine protease high te ... | 2016 | 27274359 |
| outer membrane vesicles displaying engineered glycotopes elicit protective antibodies. | the o-antigen polysaccharide (o-ps) component of lipopolysaccharides on the surface of gram-negative bacteria is both a virulence factor and a b-cell antigen. antibodies elicited by o-ps often confer protection against infection; therefore, o-ps glycoconjugate vaccines have proven useful against a number of different pathogenic bacteria. however, conventional methods for natural extraction or chemical synthesis of o-ps are technically demanding, inefficient, and expensive. here, we describe an a ... | 2016 | 27274048 |
| multiplex assay for simultaneous detection of mycoplasma genitalium and macrolide resistance using plexzyme and plexprime technology. | mycoplasma genitalium is a cause of non-gonoccocal urethritis (ngu) in men and cervicitis and pelvic inflammatory disease in women. recent international data also indicated that the first line treatment, 1 gram stat azithromycin therapy, for m. genitalium is becoming less effective, with the corresponding emergence of macrolide resistant strains. increasing failure rates of azithromycin for m. genitalium has significant implications for the presumptive treatment of ngu and international clinical ... | 2016 | 27271704 |
| chloramphenicol derivatives as antibacterial and anticancer agents: historic problems and current solutions. | chloramphenicol (cam) is the d-threo isomer of a small molecule, consisting of a p-nitrobenzene ring connected to a dichloroacetyl tail through a 2-amino-1,3-propanediol moiety. cam displays a broad-spectrum bacteriostatic activity by specifically inhibiting the bacterial protein synthesis. in certain but important cases, it also exhibits bactericidal activity, namely against the three most common causes of meningitis, haemophilus influenzae, streptococcus pneumoniae and neisseria meningitidis. ... | 2016 | 27271676 |
| novel plasmid conferring kanamycin and tetracycline resistance in the turkey-derived campylobacter jejuni strain 11601md. | in campylobacter spp., resistance to the antimicrobials kanamycin and tetracycline is frequently associated with plasmid-borne genes. however, relatively few plasmids of campylobacter jejuni have been fully characterized to date. a novel plasmid (p11601md; 44,095nt) harboring tet(o) was identified in c. jejuni strain 11601md, which was isolated from the jejunum of a turkey produced conventionally in north carolina. analysis of the p11601md sequence revealed the presence of a high-gc content cass ... | 2016 | 27268853 |
| stimulation of biofilm formation by oxidative stress in campylobacter jejuni under aerobic conditions. | 2016 | 27268722 | |
| characteristics of bacterial pathogens associated with acute diarrhea in children under 5 years of age: a hospital-based cross-sectional study. | acute diarrhea is a leading cause of morbidity and mortality in children, particularly in those under the age of 5 years. rotavirus is recognized as the leading cause of acute diarrhea in children, however, the contribution of bacterial pathogens as causative agents varies throughout the world. here we report a hospital-based prospective study to analyze the characteristics of bacterial pathogens associated with acute diarrhea in children under 5 years of age. | 2016 | 27267601 |
| the impact of oxygen on bacterial enteric pathogens. | bacterial enteric pathogens are responsible for a tremendous amount of foodborne illnesses every year through the consumption of contaminated food products. during their transit from contaminated food sources to the host gastrointestinal tract, these pathogens are exposed and must adapt to fluctuating oxygen levels to successfully colonize the host and cause diseases. however, the majority of enteric infection research has been conducted under aerobic conditions. to raise awareness of the import ... | 2016 | 27261784 |
| influence of host ecology and behavior on campylobacter jejuni prevalence and environmental contamination risk in a synanthropic wild bird species. | campylobacter jejuni is a foodborne pathogen that often leads to human infections through the consumption of contaminated poultry. wild birds may play a role in the transmission of c. jejuni by acting as reservoir hosts. despite ample evidence that wild birds harbor c. jejuni, few studies have addressed the role of host ecology in transmission to domestic animals or humans. we tested the hypothesis that host social behavior and habitat play a major role in driving transmission risk. c. jejuni in ... | 2016 | 27260356 |
| prevalence of virulence/stress genes in campylobacter jejuni from chicken meat sold in qatari retail outlets. | chicken meat from the shelves of supermarkets in qatar was tested for the presence of campylobacter spp. and the presence of five virulence genes (htrb, cdtb, clpp, cadf and ciab) was assessed in isolates. forty eight percent of the chickens provided for supermarkets by saudi (53%) and qatari (45.9%) producers were found to be contaminated and the most important factor affecting the overall prevalence of contaminated chickens was the store from which chicken samples originated. variation in prev ... | 2016 | 27258021 |
| campylobacter jejuni csra regulates metabolic and virulence associated proteins and is necessary for mouse colonization. | campylobacter jejuni infection is a leading bacterial cause of gastroenteritis and a common antecedent leading to gullian-barré syndrome. our previous data suggested that the rna-binding protein csra plays an important role in regulating several important phenotypes including motility, biofilm formation, and oxidative stress resistance. in this study, we compared the proteomes of wild type, csra mutant, and complemented csra mutant c. jejuni strains in an effort to elucidate the mechanisms by wh ... | 2016 | 27257952 |
| optimized cultivation of campylobacter concisus from gut mucosal biopsies in inflammatory bowel disease. | campylobacter concisus is a commensal of the human oral flora that has been linked to prolonged diarrhea and inflammatory bowel disease (ibd). it has been detected more often from intestinal biopsies in patients with ibd compared to healthy controls using pcr-based techniques, whereas the number of c. concisus culture-positive biopsies in previous studies has been very limited. determining the rate of viable isolates present in the gut mucosa is of great importance when evaluating the role in di ... | 2016 | 27252786 |
| contig-layout-authenticator (cla): a combinatorial approach to ordering and scaffolding of bacterial contigs for comparative genomics and molecular epidemiology. | a wide variety of genome sequencing platforms have emerged in the recent past. high-throughput platforms like illumina and 454 are essentially adaptations of the shotgun approach generating millions of fragmented single or paired sequencing reads. to reconstruct whole genomes, the reads have to be assembled into contigs, which often require further downstream processing. the contigs can be directly ordered according to a reference, scaffolded based on paired read information, or assembled using ... | 2016 | 27248146 |
| helicobacter saguini, a novel helicobacter isolated from cotton-top tamarins with ulcerative colitis, has proinflammatory properties and induces typhlocolitis and dysplasia in gnotobiotic il-10-/- mice. | a urease-negative, fusiform, novel bacterium named helicobacter saguini was isolated from the intestines and feces of cotton-top tamarins (ctts) with chronic colitis. helicobacter sp. was detected in 69% of feces or intestinal samples from 116 ctts. the draft genome sequence, obtained by illumina miseq sequencing, for h. saguini isolate mit 97-6194-5, consisting of ∼2.9 mb with a g+c content of 35% and 2,704 genes, was annotated using the ncbi prokaryotic genomes automatic annotation pipeline. h ... | 2016 | 27245408 |
| efficacy of cinnamon bark oil and cinnamaldehyde on anti-multidrug resistant pseudomonas aeruginosa and the synergistic effects in combination with other antimicrobial agents. | the emergence of drug resistant pathogens becomes a crucial problem for infectious diseases worldwide. among these bacteria, pseudomonas aeruginosa is one of which highly resists to many currently used drugs and becomes a major concern in public health. up till now, the search for potential antimicrobial agents has been still a challenge for researchers. | 2016 | 27245046 |
| quantifying potential sources of surface water contamination with campylobacter jejuni and campylobacter coli. | campylobacter is the most common causative agent of human bacterial gastroenteritis and is frequently found in surface water, where it indicates recent contamination with animal faeces, sewage effluent, and agricultural run-off. the contribution of different animal reservoirs to surface water contamination with campylobacter is largely unknown. in the netherlands, the massive poultry culling to control the 2003 avian influenza epidemic coincided with a 44-50% reduction in human campylobacteriosi ... | 2016 | 27244295 |
| circadian control of antibacterial immunity: findings from animal models. | most of the biological functions, including the immune system, are linked to circadian rhythms in living organisms. changes occurring to biological parameters as the result of these circadian rhythms can therefore affect the outcome of a disease. for decades, model organisms have proven to be a great tool to understanding biological mechanisms such as circadian cycle and immunity. in this review, we created an inventory of the use of model organisms in order to decipher the relation between circ ... | 2016 | 27242972 |
| multiplex, rapid, and sensitive isothermal detection of nucleic-acid sequence by endonuclease restriction-mediated real-time multiple cross displacement amplification. | we have devised a novel isothermal amplification technology, termed endonuclease restriction-mediated real-time multiple cross displacement amplification (et-mcda), which facilitated multiplex, rapid, specific and sensitive detection of nucleic-acid sequences at a constant temperature. the et-mcda integrated multiple cross displacement amplification strategy, restriction endonuclease cleavage and real-time fluorescence detection technique. in the et-mcda system, the functional cross primer e-cp1 ... | 2016 | 27242766 |
| chicken anti-campylobacter vaccine - comparison of various carriers and routes of immunization. | campylobacter spp, especially the species campylobacter jejuni, are important human enteropathogens responsible for millions of cases of gastro-intestinal disease worldwide every year. c. jejuni is a zoonotic pathogen, and poultry meat that has been contaminated by microorganisms is recognized as a key source of human infections. although numerous strategies have been developed and experimentally checked to generate chicken vaccines, the results have so far had limited success. in this study, we ... | 2016 | 27242755 |
| comparative resistance of bacterial foodborne pathogens to non-thermal technologies for food preservation. | in this paper the resistance of bacterial foodborne pathogens to manosonication (ms), pulsed electric fields (pefs), high hydrostatic pressure (hhp), and uv-light (uv) is reviewed and compared. the influence of different factors on the resistance of bacterial foodborne pathogens to these technologies is also compared and discussed. only results obtained under harmonized experimental conditions have been considered. this has allowed us to establish meaningful comparisons and draw significant conc ... | 2016 | 27242749 |
| intestinal microbiota of broiler chickens as affected by litter management regimens. | poultry litter is a mixture of bedding materials and enteric bacteria excreted by chickens, and it is typically reused for multiple growth cycles in commercial broiler production. thus, bacteria can be transmitted from one growth cycle to the next via litter. however, it remains poorly understood how litter reuse affects development and composition of chicken gut microbiota. in this study, the effect of litter reuse on the microbiota in litter and in chicken gut was investigated using 2 litter m ... | 2016 | 27242676 |
| [study of mutual dependence of periodontal and colonic microbiome in health and pathology using nsg-sequencing]. | by using ngs-sequencing libraries of dna from periodontal swabs with primers specific to v6 region of 16s rdna prevalence of bacterial genera and species in periodontal and colonic microbiota of patients with periodontitis of different severity and healthy donors was analyzed. hyper-colonization of the colon with akkermansia muciniphila was found to be the most important maker of negative predisposition to periodontitis (t=133,7 at р=10(-6)). this result is in a good agreement with communication ... | 2016 | 27239990 |
| miller-fisher syndrome: are anti-gad antibodies implicated in its pathophysiology? | miller-fisher syndrome (mfs) is considered as a variant of the guillain-barre syndrome (gbs) and its characteristic clinical features are ophthalmoplegia, ataxia, and areflexia. typically, it is associated with anti-gq1b antibodies; however, a significant percentage (>10%) of these patients are seronegative. here, we report a 67-year-old female patient who presented with the typical clinical features of mfs. workup revealed antibodies against glutamic acid decarboxylase (gad) in relatively high ... | 2016 | 27239355 |
| a real options approach to biotechnology investment policy-the case of developing a campylobacter vaccine to poultry. | the aim of the article is to identify and analyse public-private incentives for the development and marketing of new animal vaccines within a real options methodological framework, and to investigate how real options methodology can be utilized to support economic incentives for vaccine development in a cost-effective way. the development of a vaccine against campylobacter jejuni in poultry is applied as a case study. employing the real options methodology, the net present value of the vaccine r ... | 2016 | 27237391 |
| campylobacter in broiler slaughter samples assessed by direct count on mccda and campy-cefex agar. | campylobacter spp. cause foodborne illnesses in humans primarily through the consumption of contaminated chicken. the aim of this study was to evaluate the united states department of agriculture's (usda) recommended methodology, protocol mlg 41.02, for the isolation, identification and direct plate counting of campylobacter jejuni and c. coli samples from the broiler slaughtering process. a plating method using both mccda and campy-cefex agars is recommended to recover campylobacter cells. it i ... | 2016 | 27237112 |
| evaluation of various campylobacter-specific quantitative pcr (qpcr) assays for detection and enumeration of campylobacteraceae in irrigation water and wastewater via a miniaturized most-probable-number-qpcr assay. | campylobacter spp. are the leading cause of bacterial gastroenteritis worldwide, and water is increasingly seen as a risk factor in transmission. here we describe a most-probable-number (mpn)-quantitative pcr (qpcr) assay in which water samples are centrifuged and aliquoted into microtiter plates and the bacteria are enumerated by qpcr. we observed that commonly used campylobacter molecular assays produced vastly different detection rates. in irrigation water samples, detection rates varied depe ... | 2016 | 27235434 |
| high-level ciprofloxacin-resistant campylobacter jejuni isolates circulating in humans and animals in incheon, republic of korea. | campylobacter jejuni is one of the major causative pathogens of outbreaks or sporadic cases of diarrhoeal diseases worldwide. in this study, we compared the phenotypic and genetic characteristics of c. jejuni isolates of human and food-producing animal origins in korea and examined the genetic relatedness between these two groups of isolates. regardless of isolation source, all c. jejuni isolates harboured four virulence genes, cadf, cdtb, ciab and racr, whereas the wlan and virb11 genes were mo ... | 2016 | 27234414 |
| whole-genome sequencing of a campylobacter jejuni strain isolated from retail chicken meat reveals the presence of a megaplasmid with mu-like prophage and multidrug resistance genes. | genome sequencing of campylobacter jejuni strain t1-21 isolated from retail chicken meat revealed the presence of a chromosome of 1,565,978 bp and a megaplasmid of 82,732 bp that contains mu-like prophage and multidrug resistance genes. this is the first reported sequence of a campylobacter megaplasmid >55 kb. | 2016 | 27231378 |
| microfluidics meets metabolomics to reveal the impact of campylobacter jejuni infection on biochemical pathways. | microfluidic devices that are currently being used in pharmaceutical research also have a significant potential for utilization in investigating exposure to infectious agents. we have established a microfluidic device cultured with caco-2 cells, and utilized metabolomics to investigate the biochemical responses to the bacterial pathogen campylobacter jejuni. in the microfluidic devices, caco-2 cells polarize at day 5, are uniform, have defined brush borders and tight junctions, and form a mucus ... | 2016 | 27231016 |
| zika virus and neurology: proving cause and effect. | 2016 | 27230852 | |
| attenuation of adhesion, biofilm formation and quorum sensing of campylobacter jejuni by euodia ruticarpa. | thermophilic campylobacters are a major cause of bacterial food-borne diarrhoeal disease. adherence and biofilm formation are key elements of campylobacter jejuni persistence in unfavourable environmental conditions. the phytochemical analysis of euodia ruticarpa fruit ethanol solution extract (eree) indicated that the major compounds were evodiamine (1), rutaecarpine (2) and evocarpine (9). e. ruticarpa fruit ethanol solution extract, compounds 1 and 2 as well as a mixture of quinolinone alkalo ... | 2016 | 27230628 |
| the csra-fliw network controls polar localization of the dual-function flagellin mrna in campylobacter jejuni. | the widespread csra/rsma protein regulators repress translation by binding gga motifs in bacterial mrnas. csra activity is primarily controlled through sequestration by multiple small regulatory rnas. here we investigate csra activity control in the absence of antagonizing small rnas by examining the csra regulon in the human pathogen campylobacter jejuni. we use genome-wide co-immunoprecipitation combined with rna sequencing to show that csra primarily binds flagellar mrnas and identify the maj ... | 2016 | 27229370 |
| [the study of influence of stresses on virulence genes expression in foodborne pathogens campylobacter jejuni]. | the study of the responses to cold exposure in campylobacterjejuni (c. jejuni)--one of the most common foodborne pathogens is important for elucidating the mechanisms of acquisition of products contaminated with campylobacter, hazardous properties. these data are also necessary to create effective systems of microbiological controls at all stages of production and storage of food. 5 pairs of oligonucleotide primers were selected for detecting of genes cadf, cdtb, ciab, flaa, iama, encoding the m ... | 2016 | 27228703 |
| mechanisms of antibiotic resistance. | emergence of resistance among the most important bacterial pathogens is recognized as a major public health threat affecting humans worldwide. multidrug-resistant organisms have not only emerged in the hospital environment but are now often identified in community settings, suggesting that reservoirs of antibiotic-resistant bacteria are present outside the hospital. the bacterial response to the antibiotic "attack" is the prime example of bacterial adaptation and the pinnacle of evolution. "surv ... | 2016 | 27227291 |
| structural insights into the polyphyletic origins of glycyl trna synthetases. | glycyl trna synthetase (glyrs) provides a unique case among class ii aminoacyl trna synthetases, with two clearly widespread types of enzymes: a dimeric (α2) species present in some bacteria, archaea, and eukaryotes; and a heterotetrameric form (α2β2) present in most bacteria. although the differences between both types of glyrs at the anticodon binding domain level are evident, the extent and implications of the variations in the catalytic domain have not been described, and it is unclear wheth ... | 2016 | 27226617 |
| unique lipid anchor attaches vi antigen capsule to the surface of salmonella enterica serovar typhi. | polysaccharide capsules are surface structures that are critical for the virulence of many gram-negative pathogenic bacteria. salmonella enterica serovar typhi is the etiological agent of typhoid fever. it produces a capsular polysaccharide known as "vi antigen," which is composed of nonstoichiometrically o-acetylated α-1,4-linked n-acetylgalactosaminuronic acid residues. this glycan is a component of currently available vaccines. the genetic locus for vi antigen production is also present in so ... | 2016 | 27226298 |
| engineering the campylobacter jejuni n-glycan to create an effective chicken vaccine. | campylobacter jejuni is a predominant cause of human gastroenteritis worldwide. source-attribution studies indicate that chickens are the main reservoir for infection, thus elimination of c. jejuni from poultry would significantly reduce the burden of human disease. we constructed glycoconjugate vaccines combining the conserved c. jejuni n-glycan with a protein carrier, glycotag, or fused to the escherichia coli lipopolysaccharide-core. vaccination of chickens with the protein-based or e. coli-d ... | 2016 | 27221144 |
| a case of rare small bowel tumor in a child and review of literature. | 2015 | 27217683 | |
| htra is important for stress resistance and virulence in haemophilus parasuis. | haemophilus parasuis is an opportunistic pathogen that causes glässer's disease in swine, with polyserositis, meningitis, and arthritis. the high-temperature requirement a (htra)-like protease, which is involved in protein quality control, has been reported to be a virulence factor in many pathogens. in this study, we showed that htra of h. parasuis (hphtra) exhibited both chaperone and protease activities. finally, nickel import atp-binding protein (nike), periplasmic dipeptide transport protei ... | 2016 | 27217419 |
| an overview of food safety and bacterial foodborne zoonoses in food production animals in the caribbean region. | foodborne diseases (fbds) in the caribbean have a high economic burden. public health and tourism concerns rise along with the increasing number of cases and outbreaks registered over the last 20 years. salmonella spp., shigella spp., and campylobacter spp. are the main bacteria associated with these incidents. in spite of undertaking limited surveillance on fbd in the region, records related to bacterial foodborne zoonoses in food-producing animals and their associated epidemiologic significanc ... | 2016 | 27215411 |
| two cases of heavy chain mgus. | heavy chain diseases are rare variants of b-cell lymphomas that produce one of three classes of immunoglobulin heavy chains, without corresponding light chains. we describe two patients with asymptomatic heavy chain monoclonal gammopathy. the first patient is a 51-year-old woman with alpha paraprotein on serum immunofixation. the second case is a 46-year-old woman with gamma paraprotein on urine immunofixation. neither patient had corresponding monoclonal light chains. workup for multiple myelom ... | 2016 | 27213064 |
| effect of acidic electrolyzed water-induced bacterial inhibition and injury in live clam (venerupis philippinarum) and mussel (mytilus edulis). | the effect of acidic electrolyzed water (aew) on inactivating escherichia coli o104:h4, listeria monocytogenes, aeromonas hydrophila, vibrio parahaemolyticus and campylobacter jejuni in laboratory contaminated live clam (venerupis philippinarum) and mussel (mytilus edulis) was investigated. the initial levels of bacterial contamination were: in clam 4.9 to 5.7log10cfu/g, and in mussel 5.1 to 5.5log10cfu/g. two types of aew were used for treatment time intervals of 1 and 2h: strong (saew) with an ... | 2016 | 27208583 |
| detection of zoonotic enteropathogens in children and domestic animals in a semirural community in ecuador. | animals are important reservoirs of zoonotic enteropathogens, and transmission to humans occurs more frequently in low- and middle-income countries (lmics), where small-scale livestock production is common. in this study, we investigated the presence of zoonotic enteropathogens in stool samples from 64 asymptomatic children and 203 domestic animals of 62 households in a semirural community in ecuador between june and august 2014. multilocus sequence typing (mlst) was used to assess zoonotic tran ... | 2016 | 27208122 |
| evidence of avian and possum fecal contamination in rainwater tanks as determined by microbial source tracking approaches. | avian and possum fecal droppings may negatively impact roof-harvested rainwater (rhrw) water quality due to the presence of zoonotic pathogens. this study was aimed at evaluating the performance characteristics of a possum feces-associated (psm) marker by screening 210 fecal and wastewater samples from possums (n = 20) and a range of nonpossum hosts (n = 190) in southeast queensland, australia. the host sensitivity and specificity of the psm marker were 0.90 and 0.95 (maximum value, 1.00), respe ... | 2016 | 27208100 |
| molecular epidemiology of campylobacter coli strains isolated from different sources in new zealand between 2005 and 2014. | campylobacteriosis is one of the most important foodborne diseases worldwide and a significant health burden in new zealand. campylobacter jejuni is the predominant species worldwide, accounting for approximately 90% of human cases, followed by campylobacter coli most studies in new zealand have focused on c. jejuni; hence, the impact of c. coli strains on human health is not well understood. the aim of this study was to genotype c. coli isolates collected in the manawatu region of new zealand f ... | 2016 | 27208097 |
| demyelinating guillain-barré syndrome recurs more frequently than axonal subtypes. | guillain-barré syndrome (gbs) is considered a monophasic disorder yet recurrences occur in up to 6% of patients. we retrospectively studied an italian-japanese population of 236 gbs and 73 miller fisher syndrome (mfs) patients and searched for factors which may be associated with recurrence. a recurrent patient was defined as having at least two episodes that fulfilled the diagnostic criteria for gbs and mfs with an identifiable recovery after each episode and a minimum of 2months between episod ... | 2016 | 27206890 |
| multilocus sequence typing of campylobacter jejuni and campylobacter coli isolates from poultry, cattle and humans in nigeria. | to determine the genetic diversity of campylobacter jejuni and campylobacter coli isolates from nigeria and to identify the association between multilocus sequence types and hosts (poultry, cattle and humans). | 2016 | 27206561 |
| virulence characterisation of salmonella enterica isolates of differing antimicrobial resistance recovered from uk livestock and imported meat samples. | salmonella enterica is a foodborne zoonotic pathogen of significant public health concern. we have characterized the virulence and antimicrobial resistance gene content of 95 salmonella isolates from 11 serovars by dna microarray recovered from uk livestock or imported meat. genes encoding resistance to sulphonamides (sul1, sul2), tetracycline [tet(a), tet(b)], streptomycin (stra, strb), aminoglycoside (aada1, aada2), beta-lactam (bla tem), and trimethoprim (dfra17) were common. virulence gene c ... | 2016 | 27199965 |
| antimicrobial susceptibility profiles of human campylobacter jejuni isolates and association with phylogenetic lineages. | campylobacter jejuni is a zoonotic pathogen and the most common bacterial cause of human gastroenteritis worldwide. with the increase of antibiotic resistance to fluoroquinolones and macrolides, the drugs of choice for treatment, c. jejuni was recently classified as a serious antimicrobial resistant threat. here, we characterized 94 c. jejuni isolates collected from patients at four michigan hospitals in 2011 and 2012 to determine the frequency of resistance and association with phylogenetic lin ... | 2016 | 27199922 |
| bacterial β-kdo glycosyltransferases represent a new glycosyltransferase family (gt99). | kdo (3-deoxy-d-manno-oct-2-ulosonic acid) is an eight-carbon sugar mostly confined to gram-negative bacteria. it is often involved in attaching surface polysaccharides to their lipid anchors. α-kdo provides a bridge between lipid a and the core oligosaccharide in all bacterial lpss, whereas an oligosaccharide of β-kdo residues links "group 2" capsular polysaccharides to (lyso)phosphatidylglycerol. β-kdo is also found in a small number of other bacterial polysaccharides. the structure and functio ... | 2016 | 27199480 |
| guillain-barré syndrome with hyperreflexia and bilateral papillitis in a child. | guillain-barré syndrome (gbs) is an acute inflammatory polyneuropathy characterized by rapidly progressive symmetric weakness, and areflexia. areflexia is necessary for the diagnosis of gbs. however, recently there have been studies of hyperreflexia with axonal neuropathy form of gbs. we report a 14-year-old boy with gbs, who presented with hyperreflexia and bilateral papillitis. to the best of our knowledge, this is the first pediatric patient presenting with papillitis and hyperreflexia with a ... | 2016 | 27195040 |
| circulating gut-homing (α4β7+) plasmablast responses against shigella surface protein antigens among hospitalized patients with diarrhea. | developing countries are burdened with shigella diarrhea. understanding mucosal immune responses associated with natural shigella infection is important to identify potential correlates of protection and, as such, to design effective vaccines. we performed a comparative analysis of circulating mucosal plasmablasts producing specific antibodies against highly conserved invasive plasmid antigens (ipac, ipad20, and ipad120) and two recently identified surface protein antigens, pan-shigella surface ... | 2016 | 27193041 |
| campylobacter jejuni adenosine triphosphate phosphoribosyltransferase is an active hexamer that is allosterically controlled by the twisting of a regulatory tail. | adenosine triphosphate phosphoribosyltransferase (atp-prt) catalyzes the first committed step of the histidine biosynthesis in plants and microorganisms. here, we present the functional and structural characterization of the atp-prt from the pathogenic ε-proteobacteria campylobacter jejuni (cjeatp-prt). this enzyme is a member of the long form (hisgl ) atp-prt and is allosterically inhibited by histidine, which binds to a remote regulatory domain, and competitively inhibited by amp. in the cryst ... | 2016 | 27191057 |
| clostridium difficile enterocolitis and reactive arthritis: a case report and review of the literature. | reactive arthritis is a rare complication of clostridium difficile enterocolitis, especially in children. we review the 6 pediatric cases published in the english and non-english literature and discuss their clinical presentation, outcome, treatment, and pathophysiology. we also report the seventh case of clostridium difficile reactive arthritis in a 6-year-old boy who was treated with amoxicillin-clavulanate for 10 days because of an upper respiratory infection. after the antibiotic course, the ... | 2016 | 27190666 |
| impact of urban contamination of the la paz river basin on thermotolerant coliform density and occurrence of multiple antibiotic resistant enteric pathogens in river water, irrigated soil and fresh vegetables. | la paz river in andean highlands is heavily polluted with urban run-off and further contaminates agricultural lowlands and downstream waters at the amazon watershed. agricultural produce at this region is the main source of vegetables for the major andean cities of la paz and el alto. we conducted a 1 year study, to evaluate microbial quality parameters and occurrence of multiple enteropathogenic bacteria (enterohemorrhagic e. coli-ehec, enteroinvasive e. coli or shigella-eiec/shigella, enteroag ... | 2016 | 27186463 |
| clustered intracellular salmonella enterica serovar typhimurium blocks host cell cytokinesis. | several bacterial pathogens and viruses interfere with the cell cycle of their host cells to enhance virulence. this is especially apparent in bacteria that colonize the gut epithelium, where inhibition of the cell cycle of infected cells enhances the intestinal colonization. we found that intracellular salmonella enterica serovar typhimurium induced the binucleation of a large proportion of epithelial cells by 14 h postinvasion and that the effect was dependent on an intact salmonella pathogeni ... | 2016 | 27185791 |
| antibacterial, structural and optical characterization of mechano-chemically prepared zno nanoparticles. | structural investigations, optical properties and antibacterial performance of the pure zinc oxide (zno) nanoparticles (nps) synthesized by mechano-chemical method are presented. the morphology, dimensions and crystallinity of the zno nps were controlled by tweaking the mechanical agitation of the mixture and subsequent thermal treatment. zno nanoparticles in small (< 20 nm) dimensions with spherical morphology and narrow size distribution were successfully obtained after treating the mechano-ch ... | 2016 | 27183165 |
| comparative study on immunoglobulin y transfer from breeding hens to egg yolk and progeny chicks in different breeds of poultry. | this study was undertaken to compare the immunoglobulin y (igy) level and its efficacy in laying hens of four different breeds of poultry (viz., vanraja, gramapriya, blackrock, and kalingabrown) and its relative transfer in egg yolk and chick. | 2016 | 27182141 |
| impact of changing from staining to culture techniques on detection rates of campylobacter spp. in routine stool samples in chile. | campylobacter is a leading cause of bacterial gastroenteritis, but sensitive diagnostic methods such as culture are expensive and often not available in resource limited settings. therefore, direct staining techniques have been developed as a practical and economical alternative. we analyzed the impact of replacing campylobacter staining with culture for routine stool examinations in a private hospital in chile. | 2016 | 27177918 |
| clinical and epidemiological analysis of campylobacter fetus subsp. fetus infections in humans and comparative genetic analysis with strains isolated from cattle. | campylobacter fetus subspecies fetus (cff) is an important pathogen for both cattle and humans. we performed a systematic epidemiological and clinical study of patients and evaluated the genetic relatedness of 17 human and 17 bovine cff isolates by using different genotyping methods. in addition, the serotype, the dissemination of the genomic island containing a type iv secretion system (t4ss) and resistance determinants for tetracycline and streptomycin were also evaluated. | 2016 | 27177684 |