Publications

regulation of ribosome function following bacteriophage t4 infection.		1978	210283
slow switchover from host rna synthesis to bacteriophage rna synthesis after infection of escherichia coli with a t4 mutant defective in the bacteriophage t4-induced unfolding of the host nucleoid.	most, if not all, host rna synthesis was shut off after infection of escherichia coli strain b/5 with a bacteriophage t4 multiple mutant defective in the abilities to induce (i) unfolding of the host nucleoid (unf-), (ii) nuclear disruption (ndd-), and (iii) host dna degradation (dena-, denb-). the shutoff of host rna synthesis and turn-on of phage rna synthesis were slower after infection of e. coli with unf- phage than after infection with unf+ phage. this delay in the switchover from host rna ...	1977	201776
restoration by t4 ligase of dna sequences sensitive to "flush" cleaving restriction enzyme.	fouteen "flush"-ended segments originate from the action of the restriction endonuclease hae iii of haemophilus aegiptius on the dna of the colicinogenic factor cole 1 (a. oka and m. takanami, nature, 264, 191, 1976). they are joined by the t4 polynucleotide ligase. the reaction can be monitored by gel electrophoresis, electron microscopy and resistance to phosphatase of the 5'-32p labelled ends. the joined products are a random recombination of the original segments, and can be cleaved by the s ...	1977	198743
phosphorylation of double-stranded dnas by t4 polynucleotide kinase.	the phosphorylation by t4 polynucleotide kinase of various double-stranded dnas containing defined 5'-hydroxyl end group structures has been studied. particular emphasis was placed on finding conditions that allow complete phosphorylation. the dnas employed were homodeoxyoligonucleotides annealed on the corresponding homopolymers, dna duplexes corresponding to parts of the genes for alanine yeast trna, and a suppressor tyrosine trna from escherichia coli. the rate of phosphoylation of dnas with ...	1976	178357
effect of chloramphenicol and starvation for an essential amino acid on the synthesis and decay of t4 bacteriophage-specific messengers transcribed from early and quasi-late promoters.		1975	170861
site-specific mutagenesis using synthetic oligodeoxyribonucleotide primers: ii. in vitro selection of mutant dna.	a method for the in vitro selection of mutant dna has been devised as an adjunct to the recently developed method for the use of short enzymatically-synthesized oligodeoxyribonucleotides of defined sequence as site-specific mutagens for circular dna. the selection method uses the mutating oligodeoxyribonucleotide as a primer for escherichia coli dna polymerase i (large fragment) under conditions where there is preferential interaction with mutant dna template. after ligation using t4 dna ligase, ...	1979	161246
role of polymeric forms of the bacteriophage phi x174 coded gene a protein in phi xrfi dna cleavage.	gene a of the phi x174 genome codes for two proteins, a and a* (linney, e.a., and hayashi, m.n. (1973) nature new biol. 245, 6-8) of molecular weights 60,000 and 35,000, respectively. the phi x a* protein is formed from a natural internal initiator site within the a gene cistron while the phi x a protein is the product of the entire a gene. these two proteins have been purified to homogeneity as judged by sodium dodecyl sulfate polyacrylamide gel electrophoresis. previous studies have shown that ...	1979	158588
different specific activities of the monomeric and oligomeric forms of plasmid dna in transformation of b. subtilis and e. coli.	(1) the low residual transforming activity in preparations of monomeric, supercoiled, circular (ccc) forms of the plasmids pc194 and phv14 could be attributed to the presence in such isolates of a small number of contaminating multimeric molecules. (2) e. coli derived preparations of phv14, as in vitro recombinant plasmid capable of replication in both e. coli and b. subtilis, contain oligomeric forms of plasmid dna in addition to the prevalent monomeric ccc form. the specific transforming activ ...	1979	113646
replication of the linear mitochondrial dna of tetrahymena pyriformis.	1. electron micrographs of the linear mtdna from tetrahymena pyriformis strain gl show linear molecules with a duplex 'eye' of variable size in the middle. this indicates that replication of this dna starts near the middle of the molecule and proceeds bidirectionally to the ends, as previously shown for the mtdna of strain st (arnberg, a.c., van bruggen, e.f.j., clegg, r.a., upholt, w.b. and borst, p. (1974) biochim. biophys. acta 361, 266-276). the mtdnas of these two strains have little base s ...	1979	110348
equimolar addition of oligoribonucleotides with t4 rna ligase.	t4 induced rna ligase will join equimolar concentrations of two oligoribonucleotides, (ap)3c and p(up) 5, to form a single product, (ap)3cp(up) 5, in high yield. the presence of the 3' phosphate on p(up)5 prevents the oligomer from adding to itself. the ph optimum of the reaction is about 7.5, but less of the undesirable adenylated intermediate, app(up) 5, forms at ph 8.2. the reaction rate is a linear function of oligomer concentration from 3 micronm to 0.6 mm. the data suggest that t4 rna lig ...	1977	17097