Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| salmonella-containing vacuoles: directing traffic and nesting to grow. | salmonella enterica serovar typhimurium (s. typhimurium) is a gram-negative facultative intracellular pathogen that can infect a broad range of mammalian hosts. following invasion of host cells, the majority of s. typhimurium are known to reside in a membrane-bound compartment known as the salmonella-containing vacuole (scv). s. typhimurium actively remodels this compartment using bacterial virulence proteins, called effectors, to establish a protected niche where it can replicate. s. typhimuriu ... | 2008 | 18778407 |
| comparison of five culture methods for salmonella isolation from swine fecal samples of known infection status. | the current study was conducted to evaluate 5 bacteriologic culture methods (methods 1, 2, 3, 4, and 5) for recovery of salmonella enterica from swine feces, both for sensitivity of detection (ability to recover salmonella from a positive sample) and for specificity (not to inadvertently identify an organism as a salmonella species in a negative sample). fifty-six negative samples and 46 positive samples were processed using each of the 5 methods, which differed primarily in the combinations of ... | 2008 | 18776096 |
| salmonella enterica serovar enteritidis genes induced during oviduct colonization and egg contamination in laying hens. | salmonella enterica serovar enteritidis is the predominant serovar associated with salmonellosis worldwide, which is in part due to its ability to contaminate the internal contents of the hen's egg. it has been shown that s. enterica serovar enteritidis has an unusual tropism for the avian reproductive tract and an ability to persist in the oviduct and ovary. factors allowing s. enterica serovar enteritidis strains to contaminate eggs could be a specific interaction with the oviduct tissue, lead ... | 2008 | 18776023 |
| rapid allelic exchange in enterohemorrhagic escherichia coli (ehec) and other e. coli using lambda red recombination. | this unit describes an allelic exchange system for enterohemorrhagic e. coli (ehec), and similar pathogenic species of bacteria. the phage lambda red recombination system is expressed from a plasmid, inducing a hyper-recombinogenic state where electroporated pcr-generated substrates recombine with the bacterial chromosome at high efficiency. the technique can be used to substitute a drug marker for the gene of interest, or used to generate a clean in-frame deletion of the target gene. single gen ... | 2006 | 18770591 |
| the in vitro anti-pathogenic activity of immunoglobulin concentrates extracted from ovine blood. | an immunoglobulin-rich fraction has been prepared from ovine blood in our laboratory. we have investigated its antibacterial activity and binding activity to pathogenic whole cell antigens, lipopolysaccharide (lps) and staphylococcal enterotoxin b. ovine immunoglobulin concentrate (oic) comprised about 73 +/- 2% of igg and 11 +/- 1% of igm on a protein basis. it inhibited the growth of all 13 strains of pathogens tested, but the inhibitory activity varied according to bacterial strain. the inhib ... | 2009 | 18769875 |
| pir-b-deficient mice are susceptible to salmonella infection. | paired ig-like receptors of activating (pir-a) and inhibitory (pir-b) isoforms are expressed by many hematopoietic cells, including b lymphocytes and myeloid cells. to determine the functional roles of pir-a and pir-b in primary bacterial infection, pir-b-deficient (pir-b(-/-)) and wild-type (wt) control mice were injected i.v. with an attenuated strain of salmonella enterica typhimurium (wb335). pir-b(-/-) mice were found to be more susceptible to salmonella infection than wt mice, as evidenced ... | 2008 | 18768880 |
| a sopb deletion mutation enhances the immunogenicity and protective efficacy of a heterologous antigen delivered by live attenuated salmonella enterica vaccines. | sopb is a virulence factor of salmonella encoded by spi-5. salmonella sopb deletion mutants are impaired in their ability to cause local inflammatory responses and fluid secretion into the intestinal lumen and also can enhance the immunogenicity of a vectored antigen. in this study, we evaluated the effects on immunogenicity and the efficacy of a sopb deletion mutation on two salmonella enterica serovar typhimurium vaccine strains with different attenuating mutations expressing a highly antigeni ... | 2008 | 18765737 |
| functional analysis of the salmonella pathogenicity island 2-mediated inhibition of antigen presentation in dendritic cells. | salmonella enterica is a facultative intracellular pathogen that is able to modify host cell functions by means of effector proteins translocated by the type iii secretion system (t3ss) encoded by salmonella pathogenicity island 2 (spi2). the spi2-t3ss is also active in salmonella after uptake by murine bone marrow-derived dendritic cells (bm-dc). we have previously shown that intracellular salmonella interfere with the ability of bm-dc to stimulate antigen-dependent t-cell proliferation in an s ... | 2008 | 18765734 |
| effects of a commercially available vaccine against salmonella enterica serotype newport on milk production, somatic cell count, and shedding of salmonella organisms in female dairy cattle with no clinical signs of salmonellosis. | to determine effects of vaccination with siderophore receptor and porin (srp) proteins derived from salmonella enterica serotype newport on milk production, somatic cell count, and shedding of salmonella organisms in female dairy cattle. | 2008 | 18764698 |
| first report of a salmonella enterica serovar weltevreden outbreak on reunion island, france, august 2007. | an outbreak of gastroenteritis involving 26 guests of a wedding dinner occurred in august 2007 in réunion island, a french overseas department. salmonella was isolated in 61.5% of cases and the two isolates serotyped were of serovar weltevreden. we believe this to be the first food-borne outbreak due to s. enterica serovar weltevreden described in réunion island. the epidemiological and environmental investigations of this outbreak did not provide enough evidence to identify a single vehicle of ... | 2008 | 18761897 |
| nationwide outbreak of salmonella enterica serotype kedougou infection in infants linked to infant formula milk, spain, 2008. | 2008 | 18761885 | |
| cytolocalization of the phop response regulator in salmonella enterica: modulation by extracellular mg2+ and by the scv environment. | the phop/phoq two-component system plays an essential role regulating numerous virulence phenotypes in salmonella enterica. previous work showed that phoq, the sensor protein, switches between the kinase- and the phosphatase-dominant state in response to environmental mg2+ availability. this switch defines the phop phosphorylation status and, as a result, the transcriptional activity of this regulator. in this work, using the flash labelling technique, we examine phop cytolocalization in respons ... | 2008 | 18761685 |
| the mechanism of ciprofloxacin resistance in dihydrogen peroxide-induced mutants of salmonella enterica subsp. enterica serovar typhimurium consists mainly in mutations in gyra gene and less in mutations affecting ciprofloxacin uptake. | the effect of h(2)o(2) on the induction of ciprofloxacin (cfl) resistant mutants of salmonella enterica subsp. enterica serovar typhimurium was evaluated and determinants of cfl resistance in the mutants were analyzed. factors associated with cfl resistance in h(2)o(2)-induced mutants included (i) mutations in gyra gene, predominantly (63 %) asp(87)-->asn and less (37 %) ser(83)-->phe substitutions, (ii) mutations in the regulatory genes of marrab or soxrs or in the individual structural genes o ... | 2008 | 18759123 |
| the lysr-type transcriptional regulator hrg counteracts phagocyte oxidative burst and imparts survival advantage to salmonella enterica serovar typhimurium. | lysr-type transcriptional regulators (lttrs) are one of the key players that help bacteria adapt to different environments. we have designated stm0952, a putative lttr in salmonella enterica serovar typhimurium (s. typhimurium), as hydrogen peroxide resistance gene (hrg). a hrg knockout mutant of s. typhimurium was sensitive to oxidative products of the respiratory burst, specifically to h(2)o(2). the hrg mutant is profoundly attenuated in a murine model of infection and showed decreased intrace ... | 2008 | 18757817 |
| the salmonella spi-2 effector ssej exhibits eukaryotic activator-dependent phospholipase a and glycerophospholipid : cholesterol acyltransferase activity. | intracellular replication of salmonella enterica serovar typhimurium within membrane-bound compartments, called salmonella-containing vacuoles, depends on the activities of several effector proteins translocated by the salmonella pathogenicity island 2 (spi-2)-encoded type iii secretion system. the spi-2 effector protein ssej shows similarity at the amino acid level to several gdsl lipases with glycerophospholipid : cholesterol acyltransferase (gcat) activity. in this study, we show that catalyt ... | 2008 | 18757801 |
| antimicrobial resistance in salmonella enterica serovar heidelberg isolates from retail meats, including poultry, from 2002 to 2006. | salmonella enterica serovar heidelberg frequently causes food-borne illness in humans. there are few data on the prevalence, antimicrobial susceptibility, and genetic diversity of salmonella serovar heidelberg isolates in retail meats. we compared the prevalences of salmonella serovar heidelberg in a sampling of 20,295 meats, including chicken breast (n = 5,075), ground turkey (n = 5,044), ground beef (n = 5,100), and pork chops (n = 5,076), collected during 2002 to 2006. isolates were analyzed ... | 2008 | 18757574 |
| molecular phylogeny of the salmonellae: relationships among salmonella species and subspecies determined from four housekeeping genes and evidence of lateral gene transfer events. | the salmonellae are a diverse group of bacteria within the family enterobacteriaceae that includes two species, salmonella enterica and salmonella bongori. in order to characterize the phylogenetic relationships of the species and subspecies of salmonella, we analyzed four housekeeping genes, gapa, phop, mdh and reca, comprising 3,459 bp of nucleotide sequence data for each isolate sequenced. sixty-one isolates representing the most common serotypes of the seven subspecies of salmonella enterica ... | 2008 | 18757540 |
| direct and quantitative analysis of salmonella enterica serovar typhimurium using real-time pcr from artificially contaminated chicken meat. | for quantitative pcr assay of salmonella enterica serovar typhimurium in food samples, a real-time pcr method was developed, based on dna genome equivalent. specific primers and probe designed based on the stm4497 gene of s. typhimurium lt2 showed the specificity to s. typhimurium. threshold cycle (ct) values of real-time pcr were obtained from a quantitative standard curve with genomic dna of salmonella typhimurium. in addition, the recovery of s. typhimurium inoculated artificially to chicken ... | 2008 | 18756108 |
| occurrence and characteristics of the cytolysin a gene in shigella strains and other members of the family enterobacteriaceae. | cytolysin a (clya, hlye, shea) is a hemolytic pore-forming toxin found in escherichia coli and salmonella enterica serovars typhi and paratyphi a. in the present study, analysis of several shigella strains revealed that they harbor only nonfunctional clya gene copies that have been inactivated either by the integration of insertion sequence (is) elements (shigella dysenteriae, shigella boydii, and shigella sonnei strains) or by a frameshift mutation (shigella flexneri). shigella dysenteriae and ... | 2008 | 18754791 |
| salmonella sp group a: a rare cause of bacterascites. a case report. | bacterascites (ba) is a minimally studied and defined entity. its prognosis and clinical course are not well defined, and currently there are no management guidelines. we present a rare cause of ba in which salmonella sp group a was isolated in a 44 year old man with cirrhosis who had diarrhea and fever three days earlier. treatment with intravenous ceftriaxone was effective. | 2008 | 18753997 |
| determination of pyrophosphorylated forms of lipid a in gram-negative bacteria using a multivaried mass spectrometric approach. | lipid a isolated from several bacteria (escherichia coli, pseudomonas aeruginosa, salmonella enterica, and various strains of yersinia) showed abundant formation of pyrophosphate anions upon ion dissociation. pyrophosphate [h(3)p(2)o(7)](-) and/or [hp(2)o(6)](-) anions were observed as dominant fragments from diphosphorylated lipid a anions regardless of the ionization mode (matrix-assisted laser desorption ionization or electrospray ionization), excitation mode (collisional activation or infrar ... | 2008 | 18753624 |
| the pathogenesis of salmonella enteritidis in experimentally infected ducks: a quantitative time-course study using taqman polymerase chain reaction. | ducks were subcutaneously infected with a high-virulence strain of salmonella enterica ssp. enterica serovar enteritidis (salmonella enteritidis). the kinetics of the salmonella enteritidis genomic dna loads, the immunohistochemical localization of the bacterial antigens, and the histopathological examination in various tissues were investigated. the results showed that the time course of the appearance of the salmonella enteritidis bacterial antigens and the lesions in various tissues was coinc ... | 2008 | 18753444 |
| ecology of e. coli o157:h7 and salmonella enterica in the primary vegetable production chain. | there is an increased concern that plants might be more important as a carrier for human enteric pathogens like e. coli o157:h7 and salmonella enterica serovars than previously thought. this review summarizes the knowledge available on the ecology of e. coli o157:h7 and salmonella enterica in the primary production chain of leafy green vegetables (in particular lettuce), including manure, manure-amended soil, and crop. based on the available literature, suggestions are made for the control of th ... | 2008 | 18728991 |
| antimicrobial resistance in typhoidal and nontyphoidal salmonellae. | increasing occurrence of antimicrobial resistance in both typhoidal and nontyphoidal salmonellae is a major public health problem. recent studies documenting the occurrence and types of resistance, with particular reference to quinolones and extended spectrum cephalosporins, and new approaches to treatment are reviewed. | 2008 | 18725804 |
| suppressor analysis of the motb(d33e) mutation to probe bacterial flagellar motor dynamics coupled with proton translocation. | mota and motb form the stator of the proton-driven bacterial flagellar motor, which conducts protons and couples proton flow with motor rotation. asp-33 of salmonella enterica serovar typhimurium motb, which is a putative proton-binding site, is critical for torque generation. however, the mechanism of energy coupling remains unknown. here, we carried out genetic and motility analysis of a slowly motile motb(d33e) mutant and its pseudorevertants. we first confirmed that the poor motility of the ... | 2008 | 18723617 |
| distribution of integrons and sgi1 among antibiotic-resistant salmonella enterica isolates of animal origin. | in salmonella enterica, resistance to antibiotics can be caused by the presence of sgi1, transposons or conjugative plasmids. in this study we were interested in the relative contribution of these genetic elements to the antibiotic resistance of s. enterica isolates collected within a single year in the czech republic from animal sources. altogether 123 antibiotic-resistant isolates belonging to 16 different s. enterica serovars were classified into 3 groups according to the presence of sgi1 and ... | 2009 | 18723300 |
| effect of poultry decontaminants concentration on growth kinetics for pathogenic and spoilage bacteria. | various chemical compounds are currently under review for final approval as poultry decontaminants in the european union (eu). concentration is among the factors considered by the eu authorities in the evaluation of these treatments. the aim of this research was to compare the growth parameters for pathogenic and spoilage bacteria in presence of high and low concentrations of poultry decontaminants to assess whether such treatments could involve a potential sanitary risk for consumers. growth cu ... | 2008 | 18721678 |
| inactivation of bacteria and viruses in human urine depending on temperature and dilution rate. | source separation and reuse of human urine can decrease the environmental pollution of recipient waters and reduce the need for artificial mineral fertilisers. however, the reuse of urine introduces another pathogen transmission route that needs to be managed. the inactivation of enteric pathogens and model organisms (salmonella enterica subspecies 1 serovar typhimurium (s. typhimurium), enterococcus faecalis, bacteriophages s. typhimurium 28b, ms2 and phi x 174) by urine storage was studied at ... | 2008 | 18718625 |
| evolution of genes on the salmonella virulence plasmid phylogeny revealed from sequencing of the virulence plasmids of s. enterica serotype dublin and comparative analysis. | salmonella enterica serotype dublin harbors an approximately 80-kb virulence plasmid (psdv), which mediates systemic infection in cattle. there are two types of psdv: one is psdvu (pou1113) in strain ou7025 and the other psdvr (pou1115) in ou7409 (sd lane) and many clinical isolates. sequence analysis showed that psdvr was a recombinant plasmid (co-integrate) of psdvu and a plasmid similar to a 35-kb indigenous plasmid (pou1114) of s. dublin. most of the f-transfer region in psdvu was replaced b ... | 2008 | 18718522 |
| assessment of the stability of cell-surface components of microorganisms by maldi-tof-ms following preservation on lenticule discs. | strains representing the species campylobacter coli, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella enterica, and staphylococcus aureus were randomly selected to assess the consistency of cells preserved on lenticule discs to those archived in traditional freeze-dried ampoules. each matched pair was cultured using identical conditions and analysed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (ms) to profile the surface-associated m ... | 2008 | 18717884 |
| clya cytolysin from salmonella: distribution within the genus, regulation of expression by slya, and pore-forming characteristics. | functional homologs of the escherichia coli cytolysin a (clya, hlye, shea) gene have recently been detected in salmonella enterica serovars typhi (s. typhi) and paratyphi a (s. paratyphi a). in this study, analysis of a collection of salmonella strains showed that all s. typhi and s. paratyphi a strains tested harbor an intact copy of the corresponding clya variant, i.e. clya(sty) and clya(spaa), respectively. on the other hand, clya proved to be absent in the s. enterica serovar paratyphi b and ... | 2009 | 18715828 |
| impact of compounding error on strategies for subtyping pathogenic bacteria. | comparative-omics will identify a multitude of markers that can be used for intraspecific discrimination between strains of bacteria. it seems intuitive that with this plethora of markers we can construct higher resolution subtyping assays using discrete markers to define strain "barcodes." unfortunately, with each new marker added to an assay, overall assay robustness declines because errors are compounded exponentially. for example, the difference in accuracy of strain classification for an as ... | 2008 | 18713065 |
| [zoonoses transmitted by mouse and rat maintained as laboratory or pet animals]. | large numbers of mice (mus spp.) and rats (rattus spp.) are maintained for scientific reasons and as pet animals in germany. while laboratory animals are monitored for pathogenic agents, the hygienic status of pet animals is usually completely unknown. despite great efforts, zoonotic infections were reported even in laboratory settings, e.g. with hantavirus (seoul virus), streptobacillus moniliformis, and trichphyton mentagrophytes. however, in current reports, zoonotic infections were transmitt ... | 2008 | 18712260 |
| outbreak of salmonella enterica serotype newport in a beef cow-calf herd associated with exposure to bovine viral diarrhea virus. | severe disease and death in cows and calves affected 1 of 3 separate groups (a, b, and c) of cattle on a commercial cow-calf operation. | 2008 | 18710321 |
| non-typhoidal salmonella septic arthritis in an immunocompetent child with a pharyngeal streptococcal infection. | we report the case of an immunocompetent child who showed monoarticular arthritis and fever, preceded by pharyngitis and arthralgias. because group a beta-hemolytic streptococcus had been detected in the pharyngeal swab, erythromycin was given on admission. however, based on ultrasound examination, therapy with ceftriaxone and joint fluid drainage were promptly performed, and a rapid and full recovery followed. meanwhile, salmonella enterica infection was revealed in blood and joint fluid. our c ... | 2009 | 18707909 |
| membrane topology of the salmonella enterica serovar typhimurium group b o-antigen translocase wzx. | the o-antigen translocase, wzx, is involved in translocation of bacterial polysaccharide repeat units across the cytoplasmic membrane, and is an unusually diverse, highly hydrophobic protein, with high numbers of predicted alpha-helical transmembrane segments (tms). the salmonella enterica serovar typhimurium group b o-antigen wzx was an ideal candidate for topological study as the o-antigen gene cluster is one of only a few that have been well characterized. the topology profile prediction for ... | 2008 | 18707624 |
| effects of salmonella enterica serovar enteritidis on cellular recruitment and cytokine gene expression in caecum of vaccinated chickens. | although vaccination of poultry is a suitable method to limit human food borne gastroenteritis caused by salmonella (s.), the immune mechanisms responsible for a longer lasting protection against salmonella infection in birds are not completely understood. to reveal unique protection-related immune parameters, day-old chicks were vaccinated with a commercial live s. enteritidis vaccine and challenged with wild-type s. enteritidis 147n at day 56 of life. the bacterial cell count was determined in ... | 2008 | 18706948 |
| improvement of the live vaccine strain salmonella enterica serovar typhi ty21a for antigen delivery via the hemolysin secretion system of escherichia coli. | the attenuated salmonella enterica serovar typhi strain ty21a (ty21a) is the only attenuated live oral vaccine against typhoid fever. ty21a is also an attractive carrier for the delivery of heterologous antigens. we have used ty21a for antigen delivery via the hemolysin (hlya) secretion system of escherichia coli, the prototype of the type i secretion system (t1ss). in this study, we identified by genetic complementation that the specific mutation of rpos correlated with the hemolysin production ... | 2009 | 18706861 |
| multiple-locus variable-number tandem repeat analysis for subtyping of salmonella enterica subsp. enterica serovar enteritidis. | salmonella enterica subsp. enterica serovar enteritidis is a major food-borne pathogen that caused most of salmonella infections worldwide. s. enteritidis phage type 4 (pt4) especially presents a real challenge for the classical typing methods. we developed a simple multiple-locus variable-number tandem repeat analysis (mlva) assay based on three hypervariable variable-number tandem repeat (vntr) loci for subtyping of salmonella enteritidis. testing an arbitrary chosen strain collection of 110 s ... | 2009 | 18706857 |
| accumulation of mutants in "aging" bacterial colonies is due to growth under selection, not stress-induced mutagenesis. | several bacterial systems show behavior interpreted as evidence for stress-induced mutagenesis (adaptive mutation), a postulated process by which nongrowing cells temporarily increase their general mutation rate. theoretical considerations suggest that periodic stress-induced general mutagenesis would not be advantageous in the long term, due to the high cost of deleterious mutations. alternative explanations have been tested for very few of the systems used as evidence for stress-induced mutati ... | 2008 | 18701713 |
| salmonella enterica serotype typhi from a case of breast abscess. | enteric fever is an acute systemic febrile infection caused by salmonella enterica serotype typhi. breast abscess due to s. typhi infection is a rare sequelae. we report here a classical case of salmonella typhi infection in a rare clinical form of a breast abscess in a non-lactating immuno-compromised female. | 2007 | 18697587 |
| rama confers multidrug resistance in salmonella enterica via increased expression of acrb, which is inhibited by chlorpromazine. | salmonella enterica serovar typhimurium sl1344, in which efflux pump genes (acrb, acrd, acrf, tolc) or regulatory genes thereof (mara, soxs, rama) were inactivated, was grown in the presence of 240 antimicrobial and nonantimicrobial agents in the biolog phenotype microarray. mutants lacking tolc, acrb, and rama grew significantly worse than other mutants in the presence of 48 agents (some of which have not previously been identified as substrates of acrab-tolc) and particularly poorly in the pre ... | 2008 | 18694955 |
| [emergence of salmonella typhimurium monophasic serovar: determinants of antimicrobial resistance in porcine and human strains]. | salmonella enterica serovar typhimurium (s. typhimurium) is a common cause of non-typhoideal salmonellosis in humans and animals; since the end of '90 a monophasic serovar defined by the antigenic formula 4,5:i:- has been emerged. this study shows occurrence of monophasic serovar in italy (lombardia region) and analyses antimicrobial resistance of 147 s. typhimurium and 45 monophasic strains. multiple drug resistance (mdr) has been detected in 94,5% and 76,6% of 55 porcine and 137 human isolates ... | 2008 | 18693398 |
| pseudomonas aeruginosa as a model microorganism for investigation of chemotactic behaviors in ecosystem. | motile bacteria sense changes in the concentration of chemicals in environments and respond in a behavioral manner. this behavioral response is called chemotaxis. bacterial chemotaxis can be viewed as an important prelude to metabolism, prey-predator relationships, symbiosis, infections, and other ecological interactions in biological communities. genome analysis reveals that a large number of environmental motile bacteria possess a number of genes involved in chemosensing and chemotatic signal ... | 2008 | 18691523 |
| a singular case of prophage complementation in mutational activation of recet orthologs in salmonella enterica serovar typhimurium. | a class of mutations that suppress the recombination defects of recb mutants in salmonella enterica serovar typhimurium strain lt2 activates the normally silent recet module of the gifsy-1 prophage. allele sbce21 is a 794-bp deletion within the immunity region of the prophage. concomitant with activating recet, sbce21 stimulates gifsy-1 excision, resulting in unstable suppression. early studies found both recb suppression and its instability to depend on the presence of the related gifsy-2 proph ... | 2008 | 18689471 |
| molecular analysis of high-level ciprofloxacin resistance in salmonella enterica serovar typhi and s. paratyphi a: need to expand the qrdr region? | fourteen strains of s. typhi (n=13) and s. paratyphi a (n=1) resistant to ciprofloxacin were compared with 30 ciprofloxacin decreased-susceptibility strains on the basis of qnr plasmid analysis, and nucleotide substitutions at gyra, gyrb, parc and pare. in ciprofloxacin-resistant strains, five s. typhi and a single s. paratyphi a showed triple mutations in gyra (ser83-->phe, asp87-->asn, glu133-->gly) and a novel mutation outside the quinolone resistance determining region (qrdr) (met52-->leu). ... | 2009 | 18687156 |
| antimicrobial resistance and molecular epidemiology of salmonella rissen from animals, food products, and patients in thailand and denmark. | recently we reported increases in both the number of salmonella infections due to salmonella rissen in thailand and the isolation of this serovar from pork products in thailand. the objectives of the present study were to determine the genetic diversity and antimicrobial resistance of salmonella rissen isolates recovered from humans, food products, and animals in denmark and thailand. additionally, risk factors due to travel and consumption of specific food products were analyzed and evaluated. ... | 2008 | 18687034 |
| thic is an [fe-s] cluster protein that requires adomet to generate the 4-amino-5-hydroxymethyl-2-methylpyrimidine moiety in thiamin synthesis. | thiamin pyrophosphate is a required cofactor in all organisms. the biosynthesis of thiamin requires the independently synthesized 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate (hmp-pp) and 5-hydroxyethyl-4-methylthiazole phosphate (thz-p) moieties. in bacteria, the pyrimidine moiety is derived from 5-aminoimidazole ribotide (air), and thic is the only gene product known to be required for this conversion in vivo. we report here the purification and characterization of the thic protein ... | 2008 | 18686975 |
| detection and count of salmonella enterica in pork and poultry meat products. | 2008 | 18685979 | |
| probiotics protect mice against experimental infections. | our group has concerned itself with the study of the effect of probiotics on the resistance to infections using experimental models. here, we will focus on evidence that the ufv-h2b20 strain of lactobacillus delbrueckii var. bulgaricus may be considered a probiotic and has protective effects on mice against a variety of bacterial infections. | 2008 | 18685501 |
| serum transferrin saturation increase is associated with decrease of antibacterial activity of serum in patients with hfe-related genetic hemochromatosis. | patients with type 1 (hfe-related) genetic hemochromatosis are usually excluded from blood donation on the basis that this disease may facilitate bacterial infections. the aim of the present study was to evaluate the serum antibacterial effect against salmonella enterica typhimurium lt2 in relation to iron status. | 2008 | 18684194 |
| modeling the combined effects of enterocins a and b, lactate, and edta on the growth of salmonella at different temperatures. | the effects of enterocins a and b (produced by enterococcus faecium ctc492), lactate, and ethylenediaminetetraacetic acid (edta) on the growth of salmonella were modeled together with temperature using the response surface methodology. six serovars of salmonella enterica were inoculated (ca. 10(3) cells/ml) in brain-heart infusion broth with different levels of the studied factors and then incubated at different temperatures. the results showed that while salmonella growth was affected by all th ... | 2008 | 18683627 |
| high-throughput isolation and mapping of c. elegans mutants susceptible to pathogen infection. | we present a novel strategy that uses high-throughput methods of isolating and mapping c. elegans mutants susceptible to pathogen infection. we show that c. elegans mutants that exhibit an enhanced pathogen accumulation (epa) phenotype can be rapidly identified and isolated using a sorting system that allows automation of the analysis, sorting, and dispensing of c. elegans by measuring fluorescent bacteria inside the animals. furthermore, we validate the use of amplifluor as a new single nucleot ... | 2008 | 18682730 |
| salmonella prevalence in seafood imported into japan. | a total of 353 samples of 29 types of seafood were tested for salmonella prevalence and total microbial population. salmonella enterica serotype weltevreden was isolated from 2 of 47 black tiger prawn samples. the contamination levels of salmonella were in a range of <30 to 40 most probable number per 100 g. in addition, one sample of black tiger prawns and two samples of white shrimp were positive for salmonella inva gene on pcr assay. although the mean aerobic bacterial count was greater than ... | 2008 | 18680947 |
| aquaculture and florfenicol resistance in salmonella enterica typhimurium dt104. | 2008 | 18680675 | |
| eradication of salmonella typhimurium infection in a murine model of typhoid fever with the combination of probiotic lactobacillus fermentum me-3 and ofloxacin. | the aim of the study was to detect whether in experimental salmonella enterica typhimurium infection the probiotic lactobacillus fermentum me-3 in combination with fluoroquinolone therapy would eradicate s. typhimurium, prevent the development of liver and spleen granulomas and improve the indices of oxidative stress in the ileum mucosa. the selected bacteriological, histological and biochemical methods were applied. | 2008 | 18680567 |
| antibiotic-induced perturbations of the intestinal microbiota alter host susceptibility to enteric infection. | intestinal microbiota comprises microbial communities that reside in the gastrointestinal tract and are critical to normal host physiology. understanding the microbiota's role in host response to invading pathogens will further advance our knowledge of host-microbe interactions. salmonella enterica serovar typhimurium was used as a model enteric pathogen to investigate the effect of intestinal microbiota perturbation on host susceptibility to infection. antibiotics were used to perturb the intes ... | 2008 | 18678663 |
| novel insertion sequence- and transposon-mediated genetic rearrangements in genomic island sgi1 of salmonella enterica serovar kentucky. | salmonella genomic island 1 (sgi1) is an integrative mobilizable element that harbors a multidrug resistance (mdr) gene cluster. since its identification in epidemic salmonella enterica serovar typhimurium dt104 strains, variant sgi1 mdr gene clusters conferring different mdr phenotypes have been identified in several s. enterica serovars and classified as sgi1-a to -o. a study was undertaken to characterize sgi1 from serovar kentucky strains isolated from travelers returning from africa. severa ... | 2008 | 18676889 |
| regulation of cora mg2+ channel function affects the virulence of salmonella enterica serovar typhimurium. | the cora mg(2+) channel is the primary source of intracellular mg(2+) in salmonella enterica serovar typhimurium. in another study, we found that a strain lacking cora was attenuated in mice and also defective for invasion and replication within caco-2 epithelial cells (k. m. papp-wallace, m. nartea, d. g. kehres, s. porwollik, m. mcclelland, s. j. libby, f. c. fang, and m. e. maguire, j. bacteriol. 190:6517-6523, 2008). therefore, we further examined salmonella interaction with caco-2 epithelia ... | 2008 | 18676666 |
| the cora mg2+ channel is required for the virulence of salmonella enterica serovar typhimurium. | cora is the primary mg(2+) channel in salmonella enterica serovar typhimurium. a cora mutant is attenuated in mice and defective for invasion of and replication within epithelial cells. microarray studies show that several virulence effectors are repressed in a cora mutant strain, which ultimately manifests itself as a decrease in virulence. | 2008 | 18676664 |
| in vivo expression technology and signature-tagged mutagenesis screens for identifying mechanisms of survival of zoonotic foodborne pathogens. | high-throughput genetic screens provide great insights into the biochemistry and molecular biology of how bacteria sense, respond to, and propagate within their environments. genomics era techniques such as microarrays and proteomics have great potential to increase our understanding of how foodborne pathogens grow and survive within animal and human hosts, in the environment and foods, and during thermal and nonthermal inactivation protocols. while these techniques are incredibly useful for stu ... | 2008 | 18673072 |
| evaluation of vaccination with a commercial subunit vaccine on shedding of salmonella enterica in subclinically infected dairy cows. | to estimate the efficacy of a commercially available salmonella enterica subunit vaccine on the subclinical shedding of s enterica in dairy cattle. | 2008 | 18673036 |
| cooperation of salmonella pathogenicity islands 1 and 4 is required to breach epithelial barriers. | invasion is an important microbial virulence strategy to overcome the barrier formed by polarized epithelial cells. salmonella enterica is a food-borne pathogen that deploys a type iii secretion system for the manipulation of the actin cytoskeleton and to trigger internalization into epithelial cells. here we show that this function is not sufficient to enter polarized cells and report that penetration of epithelia from the luminal side requires both the type iii secretion system and novel virul ... | 2008 | 18671822 |
| salmonella enterica serovar gallinarum biovar gallinarum in layers: epidemiological investigations of a recent outbreak in denmark. | despite the fact that no firm evidence was obtained, epidemiological investigations of a recent danish outbreak of fowl typhoid indicated that the infection might have been introduced by contaminated crates from a german slaughterhouse. fourteen danish and five german isolates of salmonella enterica serovar gallinarum biovar gallinarum obtained from recent outbreaks in denmark and germany were characterized by phenotypic and genomic methods. phenotypic characters, plasmid profiling, plasmid rest ... | 1994 | 18671116 |
| ribotypes of salmonella enterica serovar gallinarum biovars gallinarum and pullorum. | ninety-four isolates of salmonella enterica serovar gallinarum biovar pullorum and forty-one isolates of biovar gallinarum were ribotyped using the enzymes, hindiii, ecori and smai, and a digoxigenin-labelled e. coli-derived rrna probe. using hindiii, 13 profile types were observed within biovar gallinarum and 12 within biovar pullorum. the most common types accounted for 39% of biovar pullorum isolates and 47% for biovar gallinarum. ecori digests revealed two profile types within biovar pulloru ... | 1993 | 18671057 |
| characterization of salmonella enterica serovar gallinarum biovars gallinarum and pullorum by plasmid profiling and biochemical analysis. | one-hundred-and-five strains of salmonella enterica serovar gallinarum, biovar pullorum and 43 strains of biovar gallinarum were characterized by biochemical reactions, and 95 strains of biovar pullorum and 32 strains of biovar gallinarum were examined for plasmid content. twenty-one (66%) of the strains classified as biovar gallinarum contained a 85-kb virulence plasmid. six of these strains also contained a 2.5-kb plasmid. ten strains (31%) were without plasmids. ninety-three strains (98%) bel ... | 1992 | 18670961 |
| comparative analysis of antibiotic resistance characteristics of gram-negative bacteria isolated from laying hens and eggs in conventional and organic keeping systems in bavaria, germany. | by investigating the prevalence and resistance characteristics of gram-negative bacteria from organic and conventional kept laying hens against 31 (campylobacter: 29) different antibiotics using the microdilution method, we determined to what extent different keeping systems influence bacterial resistance patterns. for this purpose, samples from 10 organic and 10 conventional flocks in bavaria (germany) were investigated four times between january 2004 and april 2005. altogether, 799 cloacal swa ... | 2008 | 18667026 |
| sdia, an n-acylhomoserine lactone receptor, becomes active during the transit of salmonella enterica through the gastrointestinal tract of turtles. | luxr-type transcription factors are typically used by bacteria to determine the population density of their own species by detecting n-acylhomoserine lactones (ahls). however, while escherichia and salmonella encode a luxr-type ahl receptor, sdia, they cannot synthesize ahls. in vitro, it is known that sdia can detect ahls produced by other bacterial species. | 2008 | 18665275 |
| differential adaptive response and survival of salmonella enterica serovar enteritidis planktonic and biofilm cells exposed to benzalkonium chloride. | this study examined the adaptive response and survival of planktonic and biofilm phenotypes of salmonella enterica serovar enteritidis adapted to benzalkonium chloride (bc). planktonic cells and biofilms were continuously exposed to 1 microg ml(-1) of bc for 144 h. the proportion of bc-adapted biofilm cells able to survive a lethal bc treatment (30 microg ml(-1)) was significantly higher (4.6-fold) than that of bc-adapted planktonic cells. similarly, there were 18.3-fold more survivors among the ... | 2008 | 18663028 |
| use of mchi encoding immunity to the antimicrobial peptide microcin h47 as a plasmid selection marker in attenuated bacterial live vectors. | live attenuated bacterial strains expressing heterologous antigens represent an attractive vaccine development strategy. however, the use of drug resistance genes for the selection of expression plasmids introduced into live vectors poses theoretical health risks. therefore, we developed a novel approach for plasmid selection based on immunity to the antimicrobial peptide microcin h47 (mcch47). two expression plasmids encoding the reporter green fluorescent protein (gfpuv) were constructed; sele ... | 2008 | 18663003 |
| high-throughput sequencing provides insights into genome variation and evolution in salmonella typhi. | isolates of salmonella enterica serovar typhi (typhi), a human-restricted bacterial pathogen that causes typhoid, show limited genetic variation. we generated whole-genome sequences for 19 typhi isolates using 454 (roche) and solexa (illumina) technologies. isolates, including the previously sequenced ct18 and ty2 isolates, were selected to represent major nodes in the phylogenetic tree. comparative analysis showed little evidence of purifying selection, antigenic variation or recombination betw ... | 2008 | 18660809 |
| subinhibitory concentrations of tetracycline affect virulence gene expression in a multi-resistant salmonella enterica subsp. enterica serovar typhimurium dt104. | treatment of salmonellosis with antibiotics is controversial and may prolong carriage and shedding. therefore, this study sought to investigate if exposure to antimicrobials influences the expression of factors involved in virulence and host colonization. the effect of subinhibitory tetracycline treatment (16 microg/ml, 30 min) on a multi-drug resistant salmonella typhimurium dt104 strain was investigated using a targeted microarray. real-time reverse transcriptase pcr was used to confirm and fu ... | 2008 | 18656407 |
| further evaluation of a multiplex pcr for differentiation of salmonella paratyphi a from other salmonellae. | salmonella enterica serovar paratyphi a is a causative agent of paratyphoid fever. the clinical syndrome caused by paratyphoid fever overlaps with other febrile illnesses and cannot be distinguished from typhoid fever. conventional methods used for diagnosis are time consuming, costly, and labor-intensive. we evaluated the specificity, sensitivity, and application of a multiplex polymerase chain reaction (pcr) previously developed by the method (ou, h.y., teh, c.s.j., thong, k.l., et al., j. mol ... | 2008 | 18653978 |
| identification of the genetic determinants of salmonella enterica serotype typhimurium that may regulate the expression of the type 1 fimbriae in response to solid agar and static broth culture conditions. | type 1 fimbriae are the most commonly found fimbrial appendages on the outer membrane of salmonella enterica serotype typhimurium. previous investigations indicate that static broth culture favours s. typhimurium to produce type 1 fimbriae, while non-fimbriate bacteria are obtained by growth on solid agar media. the phenotypic expression of type 1 fimbriae in s. typhimurium is the result of the interaction and cooperation of several genes in the fim gene cluster. other gene products that may als ... | 2008 | 18652702 |
| characterization of an outer membrane protein of salmonella enterica serovar typhimurium that confers protection against typhoid. | typhoid caused by salmonella enterica serovar typhi remains a major health concern worldwide. the emergence of multidrug-resistant strains of salmonella with increased virulence, communicability, and survivability leading to increased morbidity and mortality has further complicated its management. currently available vaccines for typhoid have less-than-desired efficacy and certain unacceptable side effects, making it pertinent to search for new immunogens suitable for vaccine formulation. the ou ... | 2008 | 18650399 |
| validation of cooking methods using shell eggs inoculated with salmonella serotypes enteritidis and heidelberg. | salmonella enterica serotype enteritidis has long been associated with eggs, and more recently, salmonella enterica serotype heidelberg has also become associated with eggs. this study was undertaken to determine whether salmonella enteritidis and salmonella heidelberg are effectively eliminated from eggs by various cooking methods. seven cooking methods were chosen--hard and soft cooked, scrambled, over easy, sunny-side up, poached, and free poached--and a pan insert and the free-flowing method ... | 2008 | 18648060 |
| comparison of the association of age with the infection of salmonella and salmonella enterica serovar typhimurium in pekin ducks and roman geese. | nontyphoid salmonella have a broad host range in poultry and mammals, and serovar typhimurium is a threat to public health. in this study, normal and sick ducks and geese were collected from 12 farms in taiwan to investigate the age-associated infection of salmonella and salmonella typhimurium in roman geese (anser anser domesticus) and pekin ducks (anas platyrhynchos domesticus). in normal birds, the prevalence of salmonella differed between species, and with age [e.g., 1-wk group, 37.5% (30/80 ... | 2008 | 18648047 |
| fowl typhoid: assessment of a disinfectant oral dose to reduce horizontal spread and mortality. | to determine the most appropriate dose in drinking water of the disinfectant n-alkyl dimethyl benzyl ammonium chloride (timsen), a fowl typhoid challenge trial was carried out using 21-day-old salmonella-free chickens. in a pretrial, performed with six groups of 10 chickens each, it was shown that the disinfectant was atoxic and safe when administered during 15 days at doses of 0 ppm, 200 ppm, 400 ppm, 800 ppm, 1600 ppm, or 3200 ppm. thereafter, a challenge trial was performed with 390 chickens ... | 2008 | 18646464 |
| correlation between viable counts of salmonella gallinarum in spleen and liver and the development of anaemia in chickens as seen in experimental fowl typhoid. | isogenic strains of salmonella enterica serovar gallinarum biovar gallinarum harbouring rflp variants of the virulence plasmid and the parent strains, were given orally to 1-week-old white leghorn cockerels. differences in viability and pattern of behaviour of the strains in the tissues were observed during the course of infection. viable counts from the spleen and liver, the development of haematological changes in the form of anaemia, and pathological lesions were shown to be correlated. none ... | 1996 | 18645897 |
| evaluation of salmonella enteritidis-immune lymphokines on host resistance to salmonella enterica ser. gallinarum infection in broiler chicks. | the prophylactic treatment of neonatal broiler chicks with lymphokines derived from s. enteritidis-immurazed chickens (se-ilk) was evaluated for its effect on the birds' resistance to an experimental infection s. enterica ser. gallinarum (sg). on the day of hatch, chicks were injected intraperitoneally with either se-ilk, control non-immune lymphokines (nilk), or were left untreated. thirty minutes later, all chicks were orally gavaged with either 10(4) colony forming units (cfu) or 10(6) cfu sg ... | 1996 | 18645895 |
| a retrospective case-control study of risk factors associated with salmonella enterica subsp. enterica serovar enteritidis infections on dutch broiler breeder farms. | the organized control of salmonella enterica subsp. enterica serovar enteritidis (se) in the dutch poultry sector aims to prevent vertical transmission by the top-down approach, but its effectiveness is now undermined by the increasing role of horizontal transmission, so that preventive hygiene on the poultry farm gains significant importance. in order to assess the actual level of preventive hygiene and to identify risk factors, an inquiry was held among a representative number of broiler breed ... | 1995 | 18645784 |
| tlr4-induced ifn-gamma production increases tlr2 sensitivity and drives gram-negative sepsis in mice. | gram-negative bacterial infection is a major cause of sepsis and septic shock. an important inducer of inflammation underlying both syndromes is the cellular recognition of bacterial products through pattern recognition receptors (prrs), including toll-like receptors (tlrs). we identified a novel antagonistic mab (named 1a6) that recognizes the extracellular portion of the tlr4-md-2 complex. if applied to mice before infection with clinical isolates of salmonella enterica or escherichia coli and ... | 2008 | 18644971 |
| mitracarpus frigidus aerial parts exhibited potent antimicrobial, antileishmanial, and antioxidant effects. | the crude extract and the hexane, ch(2)cl(2), etoac, n-buoh, and hydromethanolic fractions of the aerial parts of mitracarpus frigidus were evaluated against promastigote forms of two species of leishmania (l. chagasi and l. amazonensis), 11 strains of bacteria (staphylococcus aureus, pseudomonas aeruginosa, salmonella enterica sorovar tythimurium, shigella sonnei, klebsiella pneumoniae, escherichia coli, micrococcus luteus, enterococcus faecalis, enterobacter cloacae, streptococcus pyogenes and ... | 2009 | 18644325 |
| characterization of antibiotic resistance genes linked to class 1 and 2 integrons in strains of salmonella spp. isolated from swine. | the aim of this study was to characterize the antibiotic resistance profiles, the integron-associated resistance determinants, and the potential ability of transferring these determinants by conjugation in salmonella enterica isolated from swine. fifty-four strains of salmonella spp. were isolated from healthy swine. the percentages of resistance, determined by the plate dilution method were as follows: oxytetracycline (41%), streptomycin (39%), sulphamethoxazol+trimethoprim (19%), enrofloxacin- ... | 2008 | 18641703 |
| deletion of flagellin's hypervariable region abrogates antibody-mediated neutralization and systemic activation of tlr5-dependent immunity. | tlrs trigger immunity by detecting microbe-associated molecular patterns (mamps). flagellin is a unique mamp because it harbors 1) an antigenic hypervariable region and 2) a conserved domain involved in tlr5-dependent systemic and mucosal proinflammatory and adjuvant activities. in this study, the contribution of the flagellin domains in tlr5 activation was investigated. we showed that tlr5 signaling can be neutralized in vivo by flagellin-specific abs, which target the conserved domain. however ... | 2008 | 18641341 |
| mediation of cannabidiol anti-inflammation in the retina by equilibrative nucleoside transporter and a2a adenosine receptor. | cannabidiol (cbd), a nonpsychotropic, nontoxic compound has been shown to block diabetes- and endotoxin-induced retinal damage. however, the protective mechanism of this anti-inflammatory cannabinoid is not completely understood. the goal of this study is to determine the role of adenosine signaling in retinal inflammation and its potential modulation by cbd. | 2008 | 18641283 |
| a novel recombinant bacterial vaccine strain expressing dual viral antigens induces multiple immune responses to the gag and gp120 proteins of hiv-1 in immunized mice. | recombinant salmonella enterica serovar typhi can function as a live vector to deliver foreign antigens to the mammalian immune system and induce both mucosal and systemic immunity. in this study, we generated a recombinant salmonella typhi strain pils-pilt-gag+ (pvax1-gp120) harboring the human immunodeficiency virus (hiv) gag gene integrated into the bacterial chromosome and gp120 gene carried by a plasmid. mice inoculated with this recombinant bacterium through intranasal route produced high ... | 2008 | 18639586 |
| role of the striatum in the humoral immune response to thymus-independent and thymus-dependent antigens in rats. | since the role of striatal gabaergic medium-sized spiny (msp) neurons in the modulation of the immune responses is largely unknown, we evaluated the humoral immune response in rats with bilateral lesion of the striatum caused by quinolinic acid, which destroys msp neurons. sham-operated rats and those with striatal lesions were immunized either with tnp-lps, a t-independent antigen type 1, or one of several t-dependent antigens: ovoalbumin, bovine serum albumin, lysozyme, sheep red blood cells ( ... | 2008 | 18639583 |
| regional variations in paneth cell antimicrobial peptide expression along the mouse intestinal tract. | enteric antimicrobial peptides secreted from paneth cells, including alpha-defensins (in mice named cryptdins), are key effector molecules of innate immunity in the small intestine. the importance of paneth cells alpha-defensins emerged from studies of enteric bacterial infection in genetically modified mice, as well as from recent studies linking reduced levels of these alpha-defensins to crohn's disease localized to the ileum. however, analysis of expression of paneth cell alpha-defensins is i ... | 2008 | 18637162 |
| susceptibilities to antimicrobials and disinfectants in salmonella isolates obtained from poultry and swine in thailand. | salmonella enterica isolates from poultry (n=125) and swine (n=132) in thailand were investigated for antibiotic resistance, susceptibility to disinfectants (benzalkonium chloride (bkc), chlorhexidine digluconate (chx), zinc chloride and copper sulfate) and cyclohexane tolerance. forty-two percent were of multiple resistance to antibiotics. the minimum inhibitory concentrations (mics) of all antibiotics against isolates from swine were higher than that against the isolates from poultry. there we ... | 2008 | 18628600 |
| is nalidixic acid resistance linked to clinical virulence in salmonella enterica serovar typhi infections? | 2008 | 18628512 | |
| growth-phase regulation of lipopolysaccharide o-antigen chain length influences serum resistance in serovars of salmonella. | the amount of lipopolysaccharide (lps) o antigen (oag) and its chain length distribution are important factors that protect bacteria from serum complement. salmonella enterica serovar typhi produces lps with long chain length distribution (l-oag) controlled by the wzz gene, whereas serovar typhimurium produces lps with two oag chain lengths: an l-oag controlled by wzz(st) and a very long (vl) oag determined by wzz(fepe). this study shows that serovar enteritidis also has a bimodal oag distributi ... | 2008 | 18628492 |
| [patient with purulent pericarditis caused by salmonella enteritidis complicated by tamponade]. | we report a case of a 64-year-old male patient with a history of advanced chronic heart failure, who was admitted to our hospital with symptoms of decompensated right heart failure. chronic tamponade was diagnosed and conservative treatment was administered. due to the lack of improvement, surgical drainage of the pericardial sack was performed and salmonella enteritidis was cultured from the pericardial fluid. after a month the patient fully recovered and was discharged. | 2008 | 18626836 |
| typhoid fever. | typhoid fever is one of the most common febrile illnesses encountered by the physicians in bangladesh. diagnosis is not difficult but has lately become a challenge due to changed clinical pattern of the disease, lack of adequate facilities for blood, stool, urine culture, excessive reliance on nonspecific widal test and non availability of any reliable rapid diagnostic tests. further, the indiscriminate and injudicious use of antibiotics for treating fever in undiagnosed febrile illnesses early ... | 2008 | 18626466 |
| inhibition of salmonella enterica serovar typhimurium motility and entry into epithelial cells by a protective antilipopolysaccharide monoclonal immunoglobulin a antibody. | secretory immunoglobulin a (siga) antibodies directed against the o antigen of lipopolysaccharide (lps) are the primary determinants of mucosal immunity to gram-negative enteric pathogens. however, the underlying mechanisms by which these antibodies interfere with bacterial colonization and invasion of intestinal epithelial cells are not well understood. in this study, we report that sal4, a protective, anti-o5-specific monoclonal iga, is a potent inhibitor of salmonella enterica serovar typhimu ... | 2008 | 18625740 |
| salmonella enterica serovar typhimurium nife uptake-type hydrogenases are differentially expressed in vivo. | salmonella enterica serovar typhimurium, a common enteric pathogen, possesses three nife uptake-type hydrogenases. the results from mouse infection studies suggest that the h(2) oxidation capacity provided by these hydrogenases is important for virulence. since the three enzymes are similar in structure and function, it may be expected that they are utilized under different locations and times during an infection. a recombination-based method to examine promoter activity in vivo (rivet) was used ... | 2008 | 18625729 |
| a comparison of three molecular typing methods for the discrimination of salmonella enterica serovar infantis. | seventy-six epidemiologically unrelated salmonella enterica serovar infantis (s. infantis) isolates were typed by pulsed-field gel electrophoresis (pfge), multiple amplification of phage loci typing (maplt) and multiple-locus variable-number tandem-repeat analysis (mlva). pfge, using the restriction endonuclease xbai, generated 23 different profiles for the 76 isolates (di=0.848). maplt was undertaken using a combination of 11 primer sets based on bacteriophage sequences and generated 28 differe ... | 2008 | 18625012 |
| transcriptional expression of fljb:z66, a flagellin gene located on a novel linear plasmid of salmonella enterica serovar typhi under environmental stresses. | a previous study identified that z66+ strain of salmonella enterica serovar typhi contains two different flagellin genes, the flic encoding d or j antigen in chromosome and the fljb-like gene encoding z66 antigen in a novel linear plasmid, respectively. the promoter of fljb:z66 is different from that of flic:d/j and z66+ strain alters flagellin expression in only one orientation, from z66 to d orj antigen, raising the suspicion that z66+ strain is a special biphasic strain. to clarify the expres ... | 2008 | 18623990 |
| persistence of salmonella senftenberg in poultry production environments and investigation of its resistance to desiccation. | most salmonella serovars, including salmonella enterica subsp. enterica serovar senftenberg (s. senftenberg), are tolerant to desiccation and able to colonize and persist in feed mills. in addition, they may survive cleaning and disinfection procedures used on poultry farms. the present study was conducted to investigate the survival of s. senftenberg in broiler parent stock farms and broiler farms. the isolates from one of the parent stock farms investigated only differed by a single band in fl ... | 2008 | 18622860 |
| a comparative study on the pathogenesis of egg contamination by different serotypes of salmonella. | salmonella enterica serotype enteritidis is the predominant serotype associated with egg-borne salmonellosis in humans. apparently this serotype possesses particular characteristics that increase its chance to contaminate eggs. to identify these characteristics, two salmonella serotype enteritidis strains as well as one strain of each of the serotypes salmonella typhimurium, salmonella heidelberg, salmonella virchow and salmonella hadar strain were used to examine different aspects related to eg ... | 2008 | 18622856 |