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recognizing the real threat of biological terror.weapons of mass destruction can be used to harm and terrorize populations. such weapons include those with chemical, nuclear or biological properties. obviously computer viruses can add additional barriers to a quick response. the most effective, least costly and greatest threats are biologicals. biological terror is not new, and biological weapons have been used for centuries. however, as a result of modern technology, the risks are greater now and the outcomes more terrible. today they include ...200212053717
[contemporary control of biological warfare].biological pathogens actually are in the focus of interest as a potential biological weapon in bioterrorist's disposition. in the study the main pathogens and their activity spectrum have been presented. the special attention has been paid upon aerosol way of transmission and its consequences. the possibilities of prevention and treatment of diseases caused by biological pathogens have been also discussed.200212053581
anthrax vaccination and joint related adverse reactions in light of biological warfare scenarios.the purpose of this analysis was to evaluate anthrax vaccine (ava) and joint related adverse reactions based upon analysis of the vaers database in light of the current possibility of the use of anthrax as a biological warfare agent.200212051402
safety of anthrax vaccine: a review by the anthrax vaccine expert committee (avec) of adverse events reported to the vaccine adverse event reporting system (vaers).to assess the safety of a licensed anthrax vaccine given to nearly 400,000 us military personnel, reports of adverse events (aes) submitted to the vaccine adverse event reporting system (vaers) were reviewed and evaluated medically.200212051118
anthrax vaccine and causality assessment from individual case reports. 200212051117
bioterrorism watch. they don't call it bioterror for nothing: fear is the foe when anthrax spores are found within hospital walls. 200212046239
landscape with dead sheep: what they did to gruinard island.in the context of intensified international concern about biological weapons (bw), this article looks at the pioneering british research in this field during the second world war, which caused the long-term contamination of gruinard island in north-west scotland. public record office documents have been examined to show how scientists reported on the experiments at the time and what they thought about their (top secret) work, as well as how politicians directed their efforts and used their resul ...200212044028
responding to chemical, biological, or nuclear terrorism: the indirect and long-term health effects may present the greatest challenge.the possibility of terrorists employing chemical, biological, or nuclear/ radiological (cbn) materials has been a concern since 1995 when sarin gas was dispersed in a tokyo subway. contingency planning almost exclusively involved detection. containment, and emergency health care for mass casualties. however, it is clear that even small-scale cbn incidents--like the recent spread of anthrax spores through the mail--can cause widespread confusion, fear, and psychological stress that have lasting e ...200212043900
[anthrax due to deliberate infection]. 200212043452
protection against anthrax toxin by recombinant antibody fragments correlates with antigen affinity.the tripartite toxin produced by bacillus anthracis is the key determinant in the etiology of anthrax. we have engineered a panel of toxin-neutralizing antibodies, including single-chain variable fragments (scfvs) and scfvs fused to a human constant kappa domain (scabs), that bind to the protective antigen subunit of the toxin with equilibrium dissociation constants (k(d)) between 63 nm and 0.25 nm. the entire antibody panel showed high serum, thermal, and denaturant stability. in vitro, post-ch ...200212042864
scientists placated as us bill gets tough on bioterror. 200212037529
antimicrobial susceptibility testing of bacillus anthracis: comparison of results obtained by using the national committee for clinical laboratory standards broth microdilution reference and etest agar gradient diffusion methods.we determined the patterns of antimicrobial susceptibility of 65 isolates of bacillus anthracis (50 historical and 15 recent u.s. clinical isolates) to nine antimicrobial agents using the national committee for clinical laboratory standards (nccls) broth microdilution reference method. the results for the 50 historical b. anthracis isolates obtained by the broth microdilution method were compared to those generated by the etest agar gradient diffusion method. one isolate of b. anthracis was beta ...200212037041
bioterrorism. 200112035346
bioterrorism: an update with a focus on anthrax. 200212034576
effect of nasal immunization with protective antigen of bacillus anthracis on protective immune response against anthrax toxin.anthrax toxin consists of three proteins: protective antigen (pa), lethal factor (lf) and edema factor (ef). pa in combination with lf (lethal toxin) is lethal to mammalian cells and is the major component of human anthrax vaccine. immunization with pa elicits the production of neutralizing antibodies that form a major component of the protective immunity against anthrax. recent reports have shown that neutralizing antibody titres can serve as a reliable surrogate marker for protection against a ...200212034111
active systemic anthrax infection or lingering anthrax infection of cerebrospinal compartment? 200212033752
[sporicidal activities of disinfectants against bacillus anthracis spores]. 200212030029
biological weapons, war crimes, and wwi. 200212025830
[fingerprints of anthrax bacteria]. 200212025199
on the risk of mortality to primates exposed to anthrax spores.current events have heightened the importance of understanding the risks from inhalation exposure to small numbers of spores of bacillus anthracis. previously reported data sets have not been fully assessed using current understanding of microbial dose response. this article presents an assessment of the reported primate dose-response data. at low doses, the risk to large populations of low doses of inhaled spores (e.g., < 100) is not insignificant.200212022669
isolation, purification and partial chemical characterization of a lethal factor from common indian toad (bufo melanostictus, schneider) skin extract.indian toad (bufo melanostictus, schneider) skin extract (tse) is pharmacologically potent and probably contains several bioactive compounds [das et. al., indian j pharmacol, 28 (1996) 72]. a lethal factor was isolated and purified by neutral alumina column chromatography followed by hplc. spectroscopic (uv, ir, fab-mass) study indicated that the lethal factor (tse-lf) was a 254 da long chain compound with carbonyl, hydroxyl and ester as functional groups. ld50 of tse-lf was found to be 3.5 mg/k ...200112018580
cutaneous anthrax: an overview.the recent acts of bioterrorism have raised new questions about this uncommon disease. clinicians are puzzled as to why some of the victims exposed to bacillus anthracis spores developed the cutaneous form of the disease and others the inhalational form. despite these questions, cutaneous anthrax remains relatively simple to treat effectively. the real clinical challenge lies in the diagnosis, especially being able to distinguish it from a spider bite.200212017087
inhalational anthrax.until recently, inhalational anthrax was a medical curiosity in both the western medical literature and clinical practice. the post-september 11, 2001 outbreak of this disease in the eastern united states that spread through the mail, however, instantly changed the appreciation of this disease and the appreciation of biological terrorism/warfare in general. the microbiology, epidemiology, clinical, and therapeutic/preventative aspects of this entity, classically known as "wool sorter's disease" ...200212015917
[evaluation of the relation between the traditional index on the identification of bacillus anthracis and its virulence determinant genes].to evaluate the significance of traditional index on the identification of bacillus anthracis and its correlation with pathogenic strains.200212015097
mailborne transmission of anthrax: modeling and implications.a mathematical model is developed to analyze the transmission of inhalational anthrax through the postal system by cross-contamination of mail. the model consists of state vectors describing the numbers of cross-contaminated letters generated, the numbers of anthrax spores on these letters, the numbers of resulting infections in recipients, and matrices of transition probabilities acting on these vectors. the model simulates the recent outbreak in the united states, and provides a general framew ...200212011462
progress in rapid screening of bacillus anthracis lethal factor activity. 200212011416
monitoring anthrax vaccine safety in us military service members on active duty: surveillance of 1998 hospitalizations in temporal association with anthrax immunization.we compared 1998 hospitalizations in active-duty us military personnel for possible temporal association with anthrax immunization. immunization, demographic, and hospitalization data were analyzed using cox proportional hazards modeling for hospitalization within 42 days of vaccination. discharge diagnoses were aggregated into 14 international classification of disease, ninth revision, clinical modification (icd-9-cm) categories. approximately 11% of subjects received one or more doses of vacci ...200212009293
expression of anthrax lethal factor gene by osmolyte induction.the anthrax toxin consists of protective antigen (pa), lethal factor (lf) and edema factor (ef). pa mediates the entry of lf and ef to the cytosol where they exert their effects. although pa is the major component of the vaccines against anthrax, lf has also been found to play an important role in enhancing protective immunity. we have developed an osmolyte-inducible lf expression system. the protein expression system contributed no additional amino acids to the recombinant lf making it suitable ...200212007822
our first line of defense against bioterrorism part 2.in march, part 1 of this two-part series examined the challenges with identifying bioterrorism-specifically the anthrax outbreak. part 2 explains what's being done to equip america's health care providers to recognize and respond to future attacks, while protecting the public.200212006878
post september 11: some reflections on the role of environmental health in terrorism response. 200212004594
a primer on sampling for biological contaminants--part one: science and theory. 200212004589
anthrax sequence. useful data but no smoking gun. 200212004096
genomics and microbiology. microbial forensics--"cross-examining pathogens". 200212004075
comparative genome sequencing for discovery of novel polymorphisms in bacillus anthracis.comparison of the whole-genome sequence of bacillus anthracis isolated from a victim of a recent bioterrorist anthrax attack with a reference reveals 60 new markers that include single nucleotide polymorphisms (snps), inserted or deleted sequences, and tandem repeats. genome comparison detected four high-quality snps between the two sequenced b. anthracis chromosomes and seven differences among different preparations of the reference genome. these markers have been tested on a collection of anth ...200212004073
follow-up on "symptoms associated with anthrax exposure: suspected 'aborted' anthrax". 200212003462
variation in rrna operon number as revealed by ribotyping of bacillus anthracis strains.ribotyping of various bacillus strains with one restriction enzyme (acci) revealed significant similarity between bacillus anthracis strains, bacillus thuringiensis and bacillus cereus strains, which are all members of the bacillus cereus group. a further ribotyping study of 10 virulent and 8 attenuated b. anthracis strains, using 4 endonucleases and both 23s and 16s probes independently, was performed. the discrimination index d of hunter and gaston showed that the best combination for future l ...200212002563
post-traumatic stress disorder. 200212001959
from the centers for disease control and prevention. suspected cutaneous anthrax in a laboratory worker--texas, 2002. 200212001943
bioterrorism watch. winds of war: researchers track airborne anthrax. 200212001564
bioterrorism in australia. 200211999254
a peptide-based fluorescence resonance energy transfer assay for bacillus anthracis lethal factor protease.a fluorescence resonance energy transfer assay has been developed for monitoring bacillus anthracis lethal factor (lf) protease activity. a fluorogenic 16-mer peptide based on the known lf protease substrate mek1 was synthesized and found to be cleaved by the enzyme at the anticipated site. extension of this work to a fluorogenic 19-mer peptide, derived, in part, from a consensus sequence of known lf protease targets, produced a much better substrate, cleaving approximately 100 times more effici ...200211997440
mapping the lethal factor and edema factor binding sites on oligomeric anthrax protective antigen.assembly of anthrax toxin complexes at the mammalian cell surface involves competitive binding of the edema factor (ef) and lethal factor (lf) to heptameric oligomers and lower order intermediates of pa(63), the activated carboxyl-terminal 63-kda fragment of protective antigen (pa). we used sequence differences between pa(63) and homologous pa-like proteins to delineate a region within domain 1' of pa that may represent the binding site for these ligands. substitution of alanine for any of seven ...200211997439
the lethal and edema factors of anthrax toxin bind only to oligomeric forms of the protective antigen.the three proteins that comprise anthrax toxin, edema factor (ef), lethal factor (lf), and protective antigen (pa), assemble at the mammalian cell surface into toxic complexes. after binding to its receptor, pa is proteolytically activated, yielding a carboxyl-terminal 63-kda fragment (pa(63)) that coordinates assembly of the complexes, promotes their endocytosis, and translocates ef and lf to the cytosol. pa(63) spontaneously oligomerizes to form symmetric ring-shaped heptamers that are capable ...200211997437
case study: ed acts quickly after anthrax.after last year's anthrax attacks, eds at capital health system in trenton, nj, had to address decontamination of large numbers of patients. patients who didn't require decontamination were sent to the hospital's employee health facility so they could bypass the ed. clinicians, nursing staff, educators, and paramedics were trained in the decontamination process. direct communication with first responders was established with a portable scanner.200211995230
expected adverse events in a mass smallpox vaccination campaign.recent anthrax attacks in the united states have raised concern about the nation's vulnerability to a smallpox attack. many strategies have been suggested to minimize the impact of such an attack, ranging from quarantine and vaccination of case contacts to resumption of routine vaccination. before the latter strategy is adopted, an understanding of the likely consequences of mass vaccination is essential. count: number of adverse events resulting from two vaccination campaigns: vaccinating perso ...200211990216
anthrax and the wool trade. 1902. 200211988441
the anthrax vaccine program: an analysis of the cdc's recommendations for vaccine use.the anthrax vaccine was never proved to be safe and effective. it is one cause of gulf war illnesses, and recent vaccinees report symptoms resembling gulf war illnesses. the vaccine's production has been substandard. without adequate evaluation, the food and drug administration recently approved (retrospectively) significant changes made to the vaccine's composition since 1990. the vaccine's mandatory use for inhalation anthrax is "off-label." a skewed review of the vaccine literature by the cen ...200211988433
from woolsorters to mail sorters: anthrax past, present, and future. 200211988429
production, recovery and immunogenicity of the protective antigen from a recombinant strain of bacillus anthracis.the protective antigen (pa) is one of the three components of the anthrax toxin. it is a secreted nontoxic protein with a molecular weight of 83 kda and is the major component of the currently licensed human vaccine for anthrax. due to limitations found in the existing vaccine formulation, it has been proposed that genetically modified pa may be more effective as a vaccine. the expression and the stability of two recombinant pa (rpa) variants, pa-snke-deltaff-e308d and pa-n657a, were studied. th ...200211986925
bioterrorism's invisible threats: heightened awareness will help nurses identify real and suspected bioterrorism.ready your nursing staff for potential bioterrorism with this review of the symptoms and treatment of anthrax, smallpox, plague, tularemia, and botulism.200211984331
anthrax: is there a risk of cross-infection during endoscopy?bacillus anthracis is a large, gram-positive, spore-forming bacterium responsible for human anthrax. review of the medical literature indicates specific instrument reprocessing instructions addressing the prevention of b. anthracis transmission are limited. although nosocomial anthrax infection has not been reported, strict adherence to well-established instrument reprocessing guidelines for cleaning and high-level disinfection is recommended to prevent patient-to-patient transmission of b. anth ...200211984163
modulating airway defenses against microbes.prevention and treatment of respiratory infections remain an important health care challenge as the us population ages, contains more susceptible or high-risk people, and encounters new pathogens or antibiotic resistant bacteria. reasonably protective vaccines against very common microbes are available for childhood and adult immunization, but, generally, these are underutilized. a broader definition of higher risk individuals is evolving, which will include more for immunization. different appr ...200211981302
anthrax as a biological weapon, 2002: updated recommendations for management.to review and update consensus-based recommendations for medical and public health professionals following a bacillus anthracis attack against a civilian population.200211980524
[anthrax]. 200111980392
planning for biological disasters. occupational health nurses as "first responders".1. as a result of recent terrorist events, there is an immediate need for occupational nurses to review their disaster plans and to develop strategies to cope with bioterrorism in their workplaces. 2. the centers for disease control and prevention has identified three major categories of biological weapons. category a, which is the highest priority category (and the focus of this article), includes smallpox, anthrax, botulism, plague, tularemia, filoviruses, and adenoviruses. dealing with bioter ...200211979646
anthrax attack at the united states capitol. front line thoughts.one great fear was realized on october 15, 2001 when united states citizens witnessed firsthand the unprecedented release of anthrax into a community. although the office of the attending physician to congress had been preparing for such an unthinkable act, lessons were learned as the events unfolded. the following is a summary of the findings: preparation, planning, and frequent review of bioterrorism response procedures are essential. effective communication remains the key to successful team ...200211979645
bacterial skin infections in a tropical environment.the subject of bacterial skin infection in the tropics has shown some significant advances in the past few years, although it is still relatively understudied. of special interest is the emerging concern about the public health aspects of pyoderma, either primary or secondary to scabies, in developing countries. in addition, certain more unusual agents, such as bacillus anthracis and corynebacterium diphtheriae, continue to cause significant problems in some areas, while incidence of other tropi ...200111979120
characterization of mpf and mapk activities during meiotic maturation of xenopus tropicalis oocytes.resumption of meiosis in oocytes of xenopus tropicalis required translation but not transcription, and was marked by the appearance of a white spot and a dark ring, coincident with entry into metaphase i and the onset of anaphase i, respectively. cyclin b(2)/p34(cdc2) activity increased prior to the first meiotic division, declined at the onset of anaphase i, and subsequently increased again. the capacity of egg cytoplasm to induce germinal vesicle breakdown (gvbd) was inhibited by cycloheximide ...200211977986
iom deems anthrax vaccine safe, effective. 200211977853
biodefense. new anthrax vaccine gets a green light. 200211976419
need to know basis. 200111974821
anthrax the facts. 200111974775
anthrax and wildlife.although livestock anthrax is declining in many parts of the world, with an increasing number of countries probably truly free of the disease, anthrax remains enzootic in many national parks and even in some game ranching areas. these infected areas can present a persistent risk to surrounding livestock, which may otherwise be free of the disease, as well as a public health risk. the authors use as examples the national parks in southern africa, the wood buffalo national park in northern alberta ...200211974621
bacterial diseases of farmed deer and bison.the most important aerobic bacterial diseases of farmed deer and bison include bovine tuberculosis, johne's disease (paratuberculosis), yersiniosis, leptospirosis, brucellosis, pasteurellosis, anthrax, salmonellosis and colibacillosis. anaerobic bacterial infections affecting the same animals include necrobacillosis and a number of clostridial diseases such as tetanus, blackleg, malignant oedema and pulpy kidney. the relative importance of these diseases will vary throughout the world according ...200211974613
many leads, many dead ends. frustration inside the fbi's anthrax investigation: a so-far perfect crime. 200211974548
the esat-6/wxg100 superfamily -- and a new gram-positive secretion system?esat-6 is a small secreted protein of unknown function from mycobacterium tuberculosis that is of fundamental importance in virulence and protective immunity. a psi-blast search has identified distant homologues of esat-6 in more tractable bacteria, including bacillus subtilis, bacillus anthracis, staphylococcus aureus and clostridium acetobutylicum. the genes for esat-6-like proteins often cluster with genes encoding homologues of b. subtilis yuka. i speculate that the esat-6-like and yuka-like ...200211973144
antibody response to a delayed booster dose of anthrax vaccine and botulinum toxoid.we evaluated the prevalence and concentration of serum antibodies 18-24 months after primary inoculation with anthrax and botulinum vaccines, and assessed the reactogenicity and immunogenicity of a significantly delayed booster dose of these vaccines. five hundred and eight male active-duty military personnel received one, two or three inoculations with anthrax vaccine and/or botulinum toxoid in 1990/1991 in preparation for operations desert shield/desert storm. subjects were vaccinated with the ...200211972980
["biological" but deadly. potential biological weapons]. 200211968178
anthrax. when immunity may not be safe. 200111966151
nhs staff given advice on treating anthrax patients. 200111966091
just an accident waiting to happen. 200111966075
the anthrax scare: us healthcare as usual. 200211965205
current status of immunization against anthrax: old vaccines may be here to stay for a while.anthrax vaccination has become a 'hot' topic. on the one hand, fears that iraq holds secret caches of anthrax-based weaponry, that other countries may be developing or may have developed similar devices, or that hard-line groups may make their own anthrax-based devices for bioterrorist attacks have focused official attention on the need for means of protection, principally, though, for the military. on the other hand, the unsolved issues of the gulf war illnesses have left elements of doubt in t ...200011964777
sporulation and delta-endotoxin synthesis by bacillus thuringiensis.bacillus thuringiensis is distinguished from the very closely related bacillus cereus and bacillus anthracis by the presence of several plasmid-encoded delta-endotoxin genes. these delta-endotoxins, synthesized as protoxins, are produced in large quantities during sporulation and are packaged into intracellular inclusions. ingestion of the inclusions by insect larvae leads to protoxin solubilization and conversion to toxins each specific for one of several orders of insects. the toxins form cati ...200211964120
anthrax and anthrax anxiety: sverdlovsk revisited. 200111962347
bioterrorism. 200211961869
biodefence research. 200211961508
human antibodies against spores of the genus bacillus: a model study for detection of and protection against anthrax and the bioterrorist threat.a naive, human single-chain fv (scfv) phage-display library was used in bio-panning against live, native spores of bacillus subtilis ifo 3336 suspended in solution. a direct in vitro panning and enzyme-linked immunosorbent assay-based selection afforded a panel of nine scfv-phage clones of which two, 5b and 7e, were chosen for further study. these two clones differed in their relative specificity and affinity for spores of b. subtilis ifo 3336 vs. a panel of spores from 11 other bacillus species ...200211959974
early statistical detection of anthrax outbreaks by tracking over-the-counter medication sales.the recent series of anthrax attacks has reinforced the importance of biosurveillance systems for the timely detection of epidemics. this paper describes a statistical framework for monitoring grocery data to detect a large-scale but localized bioterrorism attack. our system illustrates the potential of data sources that may be more timely than traditional medical and public health data. the system includes several layers, each customized to grocery data and tuned to finding footprints of an epi ...200211959973
dynamic ct features of inhalational anthrax infection. 200211959702
oklahoma city and tulsa metropolitan medical response system.the tragic loss of lives on september 11 followed by the anthrax contaminations reinforced the need for work already underway through the metropolitan medical response system (mmrs). through leadership from the medical director for the medical control board and emsa, oklahoma city and tulsa have been the beneficiaries of nearly $1.5 million dollars in funding to prepare for nuclear, chemical or bioterrorism. the two oklahoma cities were among the second wave of cities invited in 1999 to particip ...200211957849
managing terror. public health officials learn lessons from bioterrorism attacks.when a photo-journalist presented with the first case of inhalation anthrax in the united states in 23 years, it marked the worse case of biological terrorism in our nation's history. it also marked a significant management challenge for numerous local, state and federal officials. review what was learned and how we can better prepare for future attacks.200211957420
calmodulin in action: diversity in target recognition and activation mechanisms.recent structural studies on calmodulin complexes with anthrax adenylyl cyclase and rat ca2+-activated k+ channel have uncovered unexpected ways by which calmodulin interacts with target proteins.200211955428
anthrax meningitis: case report. 200111953221
developmental switch of s-layer protein synthesis in bacillus anthracis.adjustment of the synthesis of abundant protein to the requirements of the cell involves processes critical to the minimization of energy expenditure. the regulation of s-layer genes might be a good model for such processes because expression must be controlled, such that the encoded proteins exactly cover the surface of the bacterium. bacillus anthracis has two s-layer genes, sap and eag, encoding the s-layer proteins sap and ea1 respectively. we report that the production and surface localizat ...200211952909
suspected cutaneous anthrax in a laboratory worker--texas, 2002.on march 6, 2002, cdc's national institute for occupational safety and health (niosh) received a request for a health hazard evaluation from the director of laboratory a to assist in the evaluation of a worker who had been diagnosed with cutaneous anthrax. laboratory a, a provisionally approved laboratory response network level b laboratory, had been processing environmental samples for bacillus anthracis in support of cdc investigations of the bioterrorist attacks in the united states during fa ...200211952281
bioterrorism watch. was anthrax mailer a bioweapons researcher? 200211951729
bioterrorism watch. building a bridge over the abyss: will bioterrorism help bring disjointed health system together? 200211951727
tracking the anthrax attacks. 200211951313
[infections by bacillus anthracis]. 200211951140
anthrax update. 200211944174
new york city, 2001: reaction and response. 200211937606
inhalational anthrax: threat, clinical presentation, and treatment.to provide nurse practitioners (nps) with a basic understanding of clinical presentation, transmission, diagnosis, pharmacological treatment, and post-exposure prophylaxis of inhalational anthrax.200111930529
bioterrorism: introduction and major agents. 200111930512
[the return of anthrax: "the evil spirit is out of the bottle"]. 200111928599
diagnosis and management of suspected cases of bioterrorism: a pediatric perspective.since october 3, 2001, the centers for disease control and prevention and other organizations have been investigating potential bioterrorist-related anthrax cases. the pediatrician may be faced with complex issues related to diagnosis and treatment of illnesses caused by intentionally released biological agents. the agents that pose a major potential bioterrorist threat are reviewed by the clinical syndromes they produce: acute respiratory distress with fever, influenza-like illnesses, acute ras ...200211927716
[female patient with cutaneous anthrax in belgium]. 200211925816
the three faces of anthrax. 200211925586
cdc director to step down march 31. 200211924204
preparing for bioterrorism. 200211924164
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