Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| studies of phospholipase and cyclooxygenase activities in herpes simplex virus type 2-transformed rat cells. | in this report we describe results showing that both arachidonic acid (20:4) release and prostaglandin (pg) synthesis in herpes simplex virus type 2 (hsv-2)-transformed tumor-derived rat fibrosarcoma (rfs) cells are inhibited by indomethacin (cyclooxygenase inhibitor). these data suggest that cyclooxygenase (prostaglandin endoperoxide synthetase, pes) and phospholipase are coupled in their regulation in rfs cells. data obtained using a cell-free assay to measure directly cyclooxygenase specific ... | 1989 | 2509090 |
| relatedness of glycoproteins expressed on the surface of simian herpes-virus virions and infected cells to specific hsv glycoproteins. | the antigenic relatedness of the surface glycoprotein antigens of six herpesviruses indigenous to human and nonhuman primates was examined. binding of anti-viral sera to viral antigens expressed on the surface of infected cells demonstrated that the surface antigens of herpes simplex virus type 1 (hsv 1), hsv 2, simian agent 8 (sa8), and herpesvirus simiae (b virus) exhibit extensive cross-reactivity. surface antigens of two viruses isolated from south american primates, h. saimiri 1 (hvs 1) and ... | 1989 | 2482016 |
| effect of 2',5'-oligoadenylate on herpes simplex virus-infected cells and preventive action of 2',5'-oligoadenylate on the lethal effect of hsv-2. | antiviral effects of ppp(a2'p)na, (2-5a) on herpes simplex virus type 1 or type 2 (hsv-1 or hsv-2)-infected baby hamster kidney fibroblasts (bhk cells) and hsv-2-infected female guinea pigs were examined. 2-5a was introduced into bhk cells in the form of a calcium phosphate precipitate and as an ointment of polyethylene glycol (peg) into guinea pig vagina. under optimum conditions, 2-5a trimer and other 2-5a derivatives inhibited over 90% of hsv-1 syncytia formation and over 50% of hsv-2 plaque ... | 1989 | 2481701 |
| effects of the immunomodulating agent r837 on acute and latent herpes simplex virus type 2 infections. | r837 is an immune modulator with no in vitro activity against herpes simplex virus (hsv). we evaluated topical r837 as therapy for genital hsv type 2 infection using the guinea pig model of this disease. furthermore, we investigated the effect of r837 therapy on acute and latent neural hsv infections. therapy initiated 12 h after viral inoculation and given twice a day significantly reduced the acute neural infection so that hsv was recovered from only 1 of 64 neural tissue specimens obtained fr ... | 1989 | 2479335 |
| properties of the herpes simplex virus type 2 trans-inducing factor vmw65 in wild-type and mutant viruses. | a temperature-sensitive mutant (ts13) of herpes simplex virus type 2 (hsv-2) has a mutation which causes in vitro thermolability of the virion. this mutation lies within the gene encoding a virion structural protein of mr 65k which is known to stimulate immediate early transcription (the trans-inducing factor, 65ktif). the results presented here show that the structural role of 65ktif is essential. the electrophoretic mobility of the 65ktif encoded by ts13 and a revertant of ts13 differed from t ... | 1989 | 2471816 |
| a subset of type-specific epitopes map in the amino terminus of herpes simplex virus type 1 glycoprotein b. | we constructed a recombinant herpes simplex virus (hsv) containing the transcribed coding and non-coding sequences of hsv-1 strain f glycoprotein b (gb) gene, a gamma 1 gene, fused to the promoter-regulatory sequences of the hsv-1 alpha 4 gene and inserted into the thymidine kinase gene of rh1g44, an hsv-1 x hsv-2 recombinant that contains an hsv-2 gb gene at the natural locus. phenotypic analyses of the insertion mutant, r3145, showed that the alpha gb gene was transcribed in the presence of cy ... | 1989 | 2471797 |
| topological distribution of virus-specific and cross-reactive antigenic determinants on the gb glycoprotein of the herpes simplex viruses. | the antigenic properties and relations between the herpes simplex virus type 1 and 2 (hsv1, hsv2) gb glycoproteins were investigated. using several assay systems to analyze the virus-specific reactivity of polyclonal monospecific rabbit anti-gb sera, it was demonstrated that most of the antigenic determinants of the gb glycoproteins exposed at the surface of both virions and infected cells are virus-specific rather than cross-reactive. comparative examination of the reactivity of human immune se ... | 1989 | 2470853 |
| targets of infection in a herpes simplex-reactivation model. | in mice surviving intracerebral inoculation with herpes simplex virus type 2 (hsv-2), recurrent infection was induced using high-dose immunosuppressive treatment. targets of reactivated infection were identified in serial sections of heads using immunoperoxidase methods to detect viral antigen. peripheral targets were cranial sensory and autonomic ganglia, peripheral nerves, and many non-neural structures. central neural tissues included retina, optic nerve and tract, lateral geniculate nucleus ... | 1989 | 2469288 |
| fine mapping of antigenic site ii of herpes simplex virus glycoprotein d. | glycoprotein d (gd) is a virion envelope component of herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) which plays an important role in viral infection and pathogenesis. previously, anti-gd monoclonal antibodies (mabs) were arranged into groups which recognize distinct type-common and type-specific sites on hsv-1 gd (gd-1) and hsv-2 gd (gd-2). several groups recognize discontinuous epitopes which are dependent on tertiary structure. three groups, vii, ii, and v, recognize continuous epitopes p ... | 1989 | 2467994 |
| enhanced efficacy of nucleoside analogs and recombinant alpha interferon in weanling mice lethally infected with herpes simplex virus type 2. | weanling icr swiss mice were inoculated intranasally with a lethal dose of herpes simplex virus type 2, and treated with either vidarabine, vidarabine-5'-monophosphate, acyclovir or a hybrid recombinant human alpha interferon which is active in murine tissues. treatment with antiviral drugs was initiated 2, 24, 48, 72 or 96 h following virus inoculation. single drug treatment showed little effect on mortality, with only acyclovir showing some slight reduction. four dual drug combinations (vidara ... | 1988 | 2455473 |
| synthesis and antiviral activity of novel n-substituted derivatives of acyclovir. | novel n-substituted derivatives of acyclovir (1a) were synthesized and evaluated for their antiviral, antimetabolic, and antitumor cell properties in vitro. monomethylation of 1a at positions 1, 7, and n-2 gave compounds 2-4, respectively. when positions 1 and n-2 were linked together by an isopropeno group, the tricyclic 9-[(2-hydroxyethoxy)methyl]-1,n-2-isopropenoguanine (5) was obtained. compound 5 was then further methylated at positions n-2 and 7 to give 6 and 7, respectively. none of the n ... | 1988 | 2455050 |
| enhanced in vitro reactivation of herpes simplex virus type 2 from latently infected guinea-pig neural tissues by 5-azacytidine. | 5-azacytidine (5-azc) reduces cytosine methylation in dna and has been reported to activate quiescent virus genes. treatment of explant cultures of latently herpes simplex virus type 2 (hsv-2)-infected guinea-pig dorsal root ganglia and spinal cords in vitro with 5-azc significantly enhanced the rate of hsv recovery. both the number of isolates from ganglia (p less than 0.001) and the rate of recovery (p less than 0.001) were significantly increased with the addition of 50 microm-5-azc to explan ... | 1988 | 2453606 |
| characterization of epitopes on native and denatured forms of herpes simplex virus glycoprotein b. | herpes simplex virus 1 glycoprotein b (gb) is an envelope glycoprotein which promotes fusion of virions with the cell membrane. this report characterizes the epitopes on native, disulfide-linked dimers of gb and monomeric forms of the glycoprotein using a panel of monoclonal antibodies. the antibodies were divided into groups, based on immune reactions with denatured or native forms of gb. the first group reacted with discontinuous epitopes assembled on gb dimers but failed to detect native or d ... | 1988 | 2453097 |
| helper activity in antigen-specific antibody production mediated by cd4+ human cytotoxic t cell clones directed against herpes simplex virus. | three hsv type 1 (hsv-1) and hsv type 2 (hsv-2) common ("hsv-type common") and three hsv-1 specific ctl clones, which were cd3+, cd4+, cd8-, 4b4+, and 2h4-, were established. these clones proliferated in response to stimulation with hsv in the presence of autologous apc. the hsv type specificity of the proliferative response was identical with that of the cytotoxic activity of the clones. the cytotoxic activity and the proliferative response were both inhibited by addition of anti-hla-dr mab to ... | 1988 | 2452188 |
| flow cytometric detection of herpes simplex virus type 2 infected and transformed cells by immunoenzymatic and by indirect immunofluorescence staining. | the glucose oxidase antiglucose oxidase (gag) immunoenzymatic staining procedure has been used to detect herpes simplex virus (hsv) antigens microscopically. in this study, the gag procedure was adapted to cells in suspension, and its potential usefulness in flow cytometry was examined. hsv-2 infected monkey kidney and hsv-2 transformed mouse cells were stained using antisera to hsv-2 or to an hsv-2 specific protein with a molecular weight of 38 kd, respectively, with the gag procedure. flow cyt ... | 1988 | 2452054 |
| the nucleotide sequence of herpes simplex virus type 2 (333) glycoprotein gb2 and analysis of predicted antigenic sites. | the gene coding for glycoprotein b2 (gb2) of herpes simplex virus type 2 (hsv-2) strain 333 was mapped and its nucleotide sequence determined. open reading frame analysis deduced a polypeptide consisting of 902 amino acids and having close homology to gb1 of hsv type 1. several predicted features of gb2 are consistent with a membrane-bound glycoprotein, i.e., a signal peptide sequence, a hydrophilic extracellular domain containing possible n-linked glycosylation sites, a hydrophobic membrane spa ... | 1987 | 2446730 |
| conserved antigenic and functional domains on glycoprotein b of herpes simplex virus 1 and bovine herpesvirus 2. brief report. | a panel of monoclonal antibodies to glycoprotein b (gb) of herpes simplex virus 1 (hsv-1) reacted in immune precipitation and in immunofluorescence tests with a glycoprotein 130,000 in apparent molecular weight specified by bovine herpesvirus 2 (bhv-2). two cross reactive antibodies neutralized the heterologous virus. these results suggest that the bhv-2 glycoprotein is the homologue of hsv-1 gb and shares structural and functional domains expressed on the gb gene product. | 1987 | 2444195 |
| enhancement of host resistance against virus infections by mtp-pe, a synthetic lipophilic muramyl peptide--i. increased survival in mice and guinea pigs after single drug administration prior to infection, and the effect of mtp-pe on interferon levels in sera and lungs. | muramyl tripeptide-phosphatidylethanolamine (mtp-pe, cgp 19835) displays prophylactic antiviral activity in mice infected with influenza viruses a and b, parainfluenza 1 virus or herpes simplex type 1 viruses (hsv/1) and in guinea pigs infected with herpes simplex type 2 viruses (hsv/2). mtp-pe is effective when given in a single intranasal dose as early as 1-4 weeks before infection. in the case of hsv/2 infections, prophylactic effectiveness can be demonstrated after a single topical applicati ... | 1986 | 2433236 |
| the effect of food dye and other environmental substances on the host defense reaction in mice in relation to virus infection. | environmental substances were examined for their effect on interferon induction and delayed type hypersensitivity (dth) responses in mice. amaranth, safrole, phenacetin and nicotine suppressed the dth response, and suppressed the serum interferon titers induced by virus infection. however, they did not affect the interferon titers which were induced by tilorone, a chemical inducer. the peak of interferon titer was 12 hours after infection with herpes simplex virus type 2 (hsv-2). therefore, amar ... | 1986 | 2432279 |
| detection of herpes simplex virus type-2 antigen(s) in biopsies from carcinoma of the uterine cervix. | indirect immunofluorescence technique was employed to detect herpes simplex virus type-2 (hsv-2) antigens in tumor biopsies from 215 patients with carcinoma of the uterine cervix. a total of 169 samples (79%) revealed brilliant nuclear fluorescence. inflammatory cells infiltrating the tumor mass were positive to 60 of the 215 patients (28%). samples showed no significant variation in the degree of fluorescence or proportion of cells binding hsv antibody with advancement in the clinical stage of ... | 1985 | 2431113 |
| localization of discontinuous epitopes of herpes simplex virus glycoprotein d: use of a nondenaturing ("native" gel) system of polyacrylamide gel electrophoresis coupled with western blotting. | previously, a panel of monoclonal antibodies (mcab) was used to define specific epitopes of herpes simplex virus glycoprotein d (gd) (r. j. eisenberg et al., j. virol. 53:634-644, 1985). three groups of antibodies recognized continuous epitopes; group vii reacted with residues 11 to 19 of the mature protein (residues 36 to 44 of the predicted sequence), group ii reacted with residues 272 to 279, and group v reacted with residues 340 to 356. four additional antibody groups recognized discontinuou ... | 1986 | 2427745 |
| antiviral effect of two aryl-oligopeptides, fr41565 and fr48217. | macrophages from mice pretreated with two chemically synthesized immunostimulating aryl-oligopeptides, fr41565 and fr48217, inhibited the multiplication of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in monkey vero cells, and that of vesicular stomatitis virus (vsv) in murine l929 cells. in addition, the aryl-oligopeptides protected mice against a lethal hsv-1 infection. in particular, when treated with fr48217 at 6 mg/kg, all mice survived, whereas all control mice died from the hsv- ... | 1986 | 2427022 |
| acyclovir treatment of disseminated herpes simplex virus type 2 infection in weanling mice: alteration of mortality and pathogenesis. | intranasal inoculation of weanling mice with herpes simplex virus type 2 (hsv-2) provides an experimental infection that closely resembles disseminated and central nervous system hsv infections of human neonates. intraperitoneal treatment with acyclovir (acv) successfully reduced mortality even when therapy was begun as late as 2 days and oral therapy as late as 4 days after viral challenge. treatment with acv beginning on day 1 completely inhibited hsv-2 replication in lung, spleen, kidney, olf ... | 1986 | 2427021 |
| detection of herpesvirus cervicovaginitis by a sequential papanicolaou--immunoperoxidase technique. | herpes simplex virus (hsv) infection constitutes an immediate threat to the neonate of pregnant women who deliver vaginally, and thus requires a rapid, specific means of diagnosis that is easily applicable to cervicovaginal smears. we applied the peroxidase-antiperoxidase technique to variously fixed, previously stained papanicolaou smears from 60 women with hsv and 18 negative controls using an hsv-2 antibody and either diaminobenzidine (dab) or aminoethylcarbazol (aec) as the chromogen and may ... | 1985 | 2424682 |
| an analysis of the biological properties of monoclonal antibodies against glycoprotein d of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization. | four monoclonal antibodies to glycoprotein d (gd) of herpes simplex virus (hsv) types 1 and 2 neutralized virus in the presence of complement but exhibited diverse activities in its absence. amino acid substitutions that conferred resistance to neutralization by each antibody were identified by deriving the nucleotide sequence of the gd gene from resistant mutants. each antibody selected a substitution from different parts of the molecule and mutants resistant to a single antibody always arose f ... | 1986 | 2423636 |
| human cytotoxic t cell clones directed against herpes simplex virus-infected cells. iv. recognition and activation by cloned glycoproteins gb and gd. | results of studies in mice and clinical observations in man indicate that t cell-mediated immunity is important in resistance to herpes simplex virus (hsv) infections. this study was undertaken to elucidate the viral antigen specificity of human hsv-immune t cells. purified hsv-1 glycoproteins gb-1 and gd-1, cloned and expressed in mammalian cells, were found to stimulate proliferation of, and interleukin 2 (il 2) production by, peripheral blood lymphocytes (pbl) of hsv seropositive individuals, ... | 1986 | 2423598 |
| human antibody response to herpes simplex virus-specific polypeptides after primary and recurrent infection. | human antibody responses to specific polypeptides of herpes simplex virus types 1 and 2 (hsv-1 and hsv-2, respectively) were assessed in serial serum specimens from 18 infected patients by immunoblot technology. nine patients had hsv-1 infections (six genital and three oral) and nine had hsv-2 genital infections. antibodies to homologous and heterologous hsv antigens were studied and correlated with total microneutralization and enzyme-linked immunosorbent assay antibodies as well as correlated ... | 1986 | 2422204 |
| herpes simplex virus specifies two subunits of ribonucleotide reductase encoded by 3'-coterminal transcripts. | we have previously described a transcription unit located between map coordinates 0.558 and 0.595 on the herpes simplex virus type 2 strain 333 genome which encodes two mrnas of 5.0 and 1.2 kilobases that share a common 3' terminus, and we have determined the nucleotide sequence of a 38,000-dalton protein specified by the smaller rna (d. a. galloway and m. a. swain, j. virol. 49:724-730, 1984). the entire nucleotide sequence of the 140,000-dalton protein specified by a 3,432-base-pair open readi ... | 1986 | 2419588 |
| comparative activities of combinations of acyclovir, vidarabine or its 5'-monophosphate, and cloned human interferons against herpes simplex virus type 2 in human and mouse fibroblast cultures. | human interferon-alpha a/d (bgl), an alpha-hybrid cloned interferon, displays activity in both human and mouse cell lines. we measured the effects of this interferon in double and triple combinations with acyclovir, vidarabine or its 5'-monophosphate against herpes simplex virus type 2 in mouse and human fibroblasts. a 75% cytopathic effect reduction assay employing a modified checkerboard technique was used. results in human fibroblasts were compared with those obtained when recombinant human i ... | 1985 | 2418779 |
| synthesis of dihydrofolate reductase and metabolism of related rna in a methotrexate resistant human cell line infected with herpes simplex virus type 2. | using a human cell line with amplified gene copy for dihydrofolate reductase (dhfr) and permissive for herpes simplex virus type 2 (hsv-2), the effect of hsv-2 on dhfr synthesis and on the steady-state level of total cellular and nuclear dhfr rna was examined. there was a reduction in dhfr synthesis accompanied by a proportional decrease in the levels of dhfr messenger rna (mrna) 1 hr after infection. both effects could be induced by hsv pretreated with ultraviolet light and this early virus ind ... | 1986 | 2418585 |
| induction of neutralizing antibody against varicella-zoster virus (vzv) by vzv gp3 and cross-reactivity between vzv gp3 and herpes simplex viruses gb. | glycoprotein gp3 (64k) is one of the major proteins specified by varicella-zoster virus (vzv). this glycoprotein was purified on an immunoadsorbent consisting of monoclonal antibody (clone 8) against gp3 linked to protein a-sepharose. rabbits were then immunized with the purified antigen to obtain monospecific antisera against gp3. the monospecific antisera and monoclonal antibody immunoprecipitated polypeptides with the same molecular weights of approximately 64,000 (64k), 106k, and 116k from a ... | 1986 | 2418583 |
| metabolism and mode of action of (r)-9-(3,4-dihydroxybutyl)guanine in herpes simplex virus-infected vero cells. | the metabolism and mode of action of the anti-herpes compound buciclovir [r)-9-(3,4-dihydroxybutyl)-guanine, bcv) has been studied in herpes simplex virus-infected and uninfected vero cells. in uninfected cells, a low and constant concentration of intracellular bcv was found, while in herpes simplex virus-infected cells, an increasing concentration of bcv phosphates was found due to metabolic trapping. the major phosphorylation product was bcv triphosphate (bcvtp) which was 92% of the total amou ... | 1986 | 2418021 |
| the antiviral activity of tetrazole phosphonic acids and their analogues. | 5-(phosphonomethyl)-1h-tetrazole and a number of related tetrazoles have been prepared and their effects on the replication of herpes simplex viruses-1 and -2 have been investigated as well as their abilities to inhibit the dna polymerases induced by these viruses and the rna transcriptase activity of influenza virus a. contrary to an earlier report, 5-(phosphonomethyl)-1h-tetrazole was not an efficient inhibitor of the replication of hsv-1 and hsv-2 in tissue culture. analogues of 5-(phosphonom ... | 1985 | 2417198 |
| antimicrobial activity of various immunomodulators: independence from normal levels of circulating monocytes and natural killer cells. | the effects of 89sr treatment on the natural host resistance of cd-1 mice and the enhancement of resistance by immunomodulators to infection with listeria monocytogenes or herpes simplex virus type 2 (hsv-2) were determined. in the cd-1 mouse, single-dose treatment with 89sr caused a profound decrease in the number of circulating monocytes (mo), lymphocytes, and polymorphonuclear leukocytes (pmn) within 1 week. there was also marked functional impairment of the mo inflammatory response, as well ... | 1986 | 2416693 |
| study of interferon production during pregnancy in mice and antiviral activity in the placenta. | although the mortality rate after herpes simplex virus type 2 inoculation was not significantly different between pregnant mice and nonpregnant mice, systemic interferon production was very high during late pregnancy compared with that in nonpregnant mice. antiviral activity was detected in placentas from all noninfected pregnant mice (80 to 320 u/ml in 20% suspension). the antiviral activity had a broad spectrum and was also effective in the cells of other species; an antiviral effect was shown ... | 1985 | 2413760 |
| expression in bacteria of gb-glycoprotein-coding sequences of herpes simplex virus type 2. | a plasmid with an insert that encodes the glycoprotein b(gb) gene of herpes simplex virus type 2 (hsv-2) has been isolated. dna sequences coding for a portion of the hsv-2 gb peptide were cloned into a bacterial lacz alpha expression vector and used to transform escherichia coli. upon induction of laczpo-promoted transcription, some of the bacteria became filamentous and produced inclusion bodies containing a large amount of a 65-kdal peptide that was shown to be precipitated by broad-spectrum a ... | 1985 | 2412940 |
| enhancement of non-specific resistance to viral infection by muramyldipeptide and its analogs. | antiviral activity of muramyldipeptide (mdp) and its lipophilic derivatives, b30-mdp and mdp-lys(l18), was investigated in mice infected with vaccinia virus (vv) and herpes simplex virus type 2 (hsv-2). mice administered these compounds subcutaneously or orally were protected against vv in tail lesion tests and against hsv-2 in skin lesion tests, respectively. since in vitro antiviral activity was not demonstrated with these compounds in cultured mammalian cells infected with either vv or hsv-2, ... | 1985 | 2412489 |
| immunoblotting and elisa analysis of hsv-1 and hsv-2-specified polypeptides by using an immunoblocking method. | herpes simplex virus (hsv) specified polypeptide antigens were analyzed by immunoblotting and enzyme-linked immunosorbent assay (elisa) methods. in general, the virus specified proteins showed marked cross-reactivity. however, the hsv-1 specified glycoprotein c could be detected by hsv-1 infected rabbit antiserum only. two low molecular weight polypeptides showed considerable reactivity with hsv-2 induced immune sera as revealed by an immunoblocking modification of the immunoblotting method. the ... | 1985 | 2412480 |
| immunity to herpes simplex virus: virus reactivation modulates lymphokine activity. | cell-mediated immunity to herpes simplex virus (hsv) was studied in patients with recurrent disease and in seropositive controls. supernatants from cultures of peripheral blood mononuclear cells (pbls) obtained during recrudescence (zero to three days after onset of lesions), convalescence (four to 14 days), and quiescence (greater than 14 days) were assayed for their ability to augment natural killer activity of normal pbls against k562 target cells. supernatants obtained during recrudescence, ... | 1985 | 2411825 |
| production of lymphokines and interferon by immune cells involved in recovery of mice from herpes simplex virus type 2 hepatitis. | adoptive transfer of spleen cells from mice 4 days after infection with herpes simplex virus type 2 (hsv-2) reduced the virus titer in the liver of recipient mice infected 24 h before transfer. macrophage chemotactic factor (cf) and macrophage migration inhibition factor (mif) were produced by day 3 of infection in spleen cell cultures stimulated with hsv-2, but not with control antigen, i.e. 1 day before the cells are active in adoptive transfer. interferon was produced in cultures established ... | 1985 | 2408997 |
| herpes simplex virus infection of the hand. | the first episode of herpes simplex virus (hsv) infection of the hand can occur in association with infection at another site, such as primary hsv-1 gingivostomatitis. autoinoculation is the likely route of transmission. the attacks are usually solitary and last about 14 days. a first episode that results from exogenous inoculation may be severely inflammatory and may last up to 28 days. the majority of cases are recurrent infections and most often occur in adults with hsv-2 infection. | 1990 | 2405025 |
| daily stress and recurrence of genital herpes simplex. | it is widely believed that emotional stress triggers recurrences of both oral and genital herpes simplex virus. on a daily basis, we studied 64 subjects prospectively for 1 to 3 months; all subjects had at least one culture-proved recurrence of genital herpes simplex virus type 2 before or during the study. all subjects filled out a questionnaire that measured psychological/emotional stress in six areas: physical health, relations with friends, relations with family, relations with sex partner(s ... | 1990 | 2393320 |
| physical and biological characterization of antigen presenting liposome formulations: relative efficacy for the treatment of recurrent genital hsv-2 in guinea pigs. | antigen presenting liposomes (apls) containing both liposome encapsulated (44%) and free (56%) recombinant glycoprotein d of herpes simplex virus type-2 (rgd-2) were characterized with respect to the interaction of the antigen with the lipid bilayer and the biological activities provided by each form of rgd-2. we found that free rgd-2 added externally to empty liposomes exhibited some biological activities both in vitro and in vivo, although we could not detect any significant adsorption and/or ... | 1990 | 2383000 |
| seroepidemiology of chlamydia in costa rica. | a population-based study of the sero-epidemiology of chlamydia was performed among a nationally representative sample of 760 costa rican women aged 25 to 59 years. interviews and sera collection were completed between september 1984 and february 1985. the overall seroprevalence of chlamydial antibodies among these women was 56.1%. women 25 to 39 years of age had a seroprevalence of 51.1%, while women 40 to 59 years of age had a seroprevalence of 64.2%. women who reported no prior sexual activity ... | 1990 | 2370061 |
| is there an association between fetal viral infection and fetal malformation? i. detection of specific igm antibodies in the serum of malformed fetuses. | determinations of igg and igm antibodies specific for cytomegalovirus (cmv), herpes simplex virus (hsv1), herpes simplex virus (hsv2), varicella-zoster virus (vzv), rubella, echo, coxsackie and morbilli viruses were performed in 20 sera from malformed fetuses. demonstration of a fetal infection by increased fetal serum igm permits linkage to a detected fetal malformation. in parallel, 14 maternal sera and 17 amniotic fluid samples were examined. laser nephelometry (a quantitative method) was use ... | 1990 | 2366130 |
| treatment of resistant herpes simplex virus with continuous-infusion acyclovir. | two patients with acquired immunodeficiency syndrome who developed severe ulcerative proctitis caused by herpes simplex virus type 2 that was resistant to acyclovir were successfully treated with 6 weeks of high-dose, continuous-infusion acyclovir sodium (1.5 to 2.0 mg/kg per hour). viruses cultured from the lesions were resistant to acyclovir in vitro after the patients had received prolonged therapy with oral and intravenous acyclovir in traditional divided doses. investigation into the mechan ... | 1990 | 2308204 |
| viral infections and recurrences of febrile convulsions. | to determine whether complicated febrile seizures occur more often in children with a proven viral infection, we performed viral examinations on 144 children with febrile convulsions, of whom 112 had simple and 32 had complicated seizures. a diagnosis of virus infection was verified in 46% of the former patients and 53% of the latter. three adenoviruses, one parainfluenza virus type 2 and one type 3, one respiratory syncytial virus, one echovirus type 11, one herpes simplex virus type 2, and one ... | 1990 | 2299489 |
| anesthesia for cesarean delivery in patients with herpes simplex virus type-2 infections. | some concerns exist regarding the use of regional anesthesia for patients with active genital herpes simplex virus type-2 (hsv) infections undergoing cesarean delivery. previous studies have reported the safe use of epidural anesthesia in these patients; there are no reports of the use of spinal anesthesia in this setting. we performed a 6-year retrospective survey of 169 patients delivered by cesarean delivery because of hsv infection. one hundred sixty-four of these patients had the diagnosis ... | 1990 | 2271471 |
| ulcerative lesions and herpes simplex virus type 2 in a patient with evan's syndrome. | we have described a case of herpes genitalis in a patient with evans's syndrome who had violaceous macules leading to deep ulcerations. this case serves as a reminder that hsv infection may be atypical and have an unusual course in immunocompromised patients. with the availability of specific therapy for this infection, it is important to consider hsv in the differential diagnosis of all ulcerative skin lesions. | 1990 | 2251541 |
| genital herpes simplex virus infections. | there has been a dramatic increase in patient visits to physicians for evaluation and treatment of genital herpes infections. this has resulted in part from an increase in genital herpes infections, particularly severe, first-episode genital herpes infections in adults without prior hsv-1 infection. virus culture remains the most sensitive and specific method for diagnosis, and use of viral cultures is encouraged. type-specific antibody tests have been employed in studies documenting the role of ... | 1990 | 2246948 |
| herpes simplex virus type 2 and other genital ulcerative infections as a risk factor for hiv-1 acquisition. | we studied the role of genital ulcerative infections for acquisition of human immunodeficiency virus type 1 (hiv-1) infection in a cohort of 989 homosexual men in amsterdam between october 1984 and december 1988. among 53 hiv-1 seroconverters serological and anamnestic data were gathered regarding herpes simplex virus type 2 (hsv-2) and syphilis in the 6 months before seroconversion. for statistical analysis a control who remained seronegative during the same interval was selected at random for ... | 1990 | 2245979 |
| cervical cancer: aetiology, earlier diagnosis and treatment. | cervical cancer which is one of the commonest genital malignancies in women worldwide including singapore is reviewed. there is a decreased incidence and mortality rate from cervical cancer with well organised population-based screening programmes using cervical smears. strong evidence exists for a sexually transmitted etiologic agent in cervical cancer. the evidence of an association between herpes simplex virus type 2 (hsv-2) and certain human papillomavirus (hpv) infections especially hpv 16 ... | 1990 | 2189347 |
| double-blind, placebo-controlled trial of a herpes simplex virus type 2 glycoprotein vaccine in persons at high risk for genital herpes infection. | to determine the efficacy of a herpes simplex virus type 2 (hsv-2) glycoprotein subunit vaccine, vaccine (50 micrograms) or placebo was administered intramuscularly at weeks 0, 4, and 22 to 161 persons who lacked hsv-2 antibodies and were sex partners of persons with recurrent genital herpes. the annual rate of acquisition of hsv infection was similar among vaccine and placebo recipients (10.7% and 8%, respectively) but was higher in initially seronegative subjects (15.5%) than in those with hsv ... | 1990 | 2181031 |
| search for herpes simplex virus 2 and human papillomavirus genetic expression in vulvar neoplasia. | specimens from vulvar carcinomas and vulvar intraepithelial neoplasia (vin) of various degrees were analyzed for the presence of herpes simplex virus 2 (hsv 2) and human papillomavirus (hpv) genetic information. a search for the hpv 16 e6 protein as well as the hsv 2 antigenic determinant la1 and icsp 11/12 protein was carried out with an immunoperoxidase assay on 12 vulvar carcinomas and 6 vins. seven invasive cancers and four vins were screened for the presence of homology to hpv 16 dna and hs ... | 1990 | 2178189 |
| [infections caused by viruses and chlamydia trachomatis on uterine cervix]. | a study on the infections caused by hpv-16, hsv-2, cmv and chlamydia trachomatis (ct) on the uterine cervices were carried out among 188 normal cases and 544 patients with chronic cervicitis using elisa, virus isolation and/or dna hybridization methods. the detection rates of these four pathogens in erosive cervices were 23.9% (64/268), 26.5% (99/374), 14.0% (22/157) and 13.4% (18/134), respectively, which were significantly higher than that in normal cervices. the total detection rate of four p ... | 1990 | 2178079 |
| antiviral activity and pharmacokinetics of hoe 602, an acyclic nucleoside, in animal models. | the acyclic nucleoside derivative hoe 602 (2-amino-9-[1,3-bis(isopropoxy)-2-propoxymethyl]purine) was evaluated for its antiviral activity in cell culture and for its therapeutic efficacy in mice infected with herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) or with murine cytomegalovirus (mcmv). hoe 602 was inactive in vitro against a variety of dna- and rna-viruses. however it prevented symptoms and mortality in mice systemically infected with hsv-1, hsv-2 or mcmv when administered intra ... | 1990 | 2177317 |
| inactivation of the shutoff gene (ul41) of herpes simplex virus types 1 and 2. | gene ul41 of herpes simplex virus type 1 (hsv-1) and the corresponding gene of hsv-2, which control the virion-mediated early suppression of cellular protein synthesis, have been inactivated by inserting a beta-galactosidase expression cassette into their coding regions. the resulting recombinants grew well in tissue culture, although with the type 2 recombinant viral protein synthesis was slightly delayed. as a result of inactivation of ul41 host protein synthesis was not suppressed in the pres ... | 1990 | 2177088 |
| cytopathogenicity, drug susceptibility, and thymidine kinase activity of a retinovirulent herpes simplex virus type 2. | we investigated some of the biological and biochemical characteristics of a neuroinvasive, retinovirulent herpes simplex virus type 2 strain sl (hsv-2[sl]) and compared them with those of a neurovirulent, nonretinovirulent hsv-2 (186). hsv-2(sl) was shown to spread rapidly and produce large syncytium in vitro. hsv-2(sl) and hsv-2(186) were equally susceptible to acyclovir (acv) and thymine arabinoside (ara-t). however, hsv-2(sl) was fourfold and 44-fold more susceptible than hsv-2(186) to iodode ... | 1990 | 2176674 |
| use of the pbs2 uracil-dna glycosylase inhibitor to differentiate the uracil-dna glycosylase activities encoded by herpes simplex virus types 1 and 2. | the bacteriophage pbs2 encoded uracil-dna glycosylase (ung) inhibitor was examined for its effect upon the nuclear ung activities of kb, hela, and vero cells infected with herpes simplex virus (hsv) type 1 or 2 and mock-infected cells. ung activity from hsv-1 infected cells exhibited the greatest sensitivity to inhibition by the inhibitor, while ung activity from cells infected with hsv-2 exhibited the greatest resistance. this differential effect was dependent upon the virus, cell line, and buf ... | 1990 | 2176221 |
| sero-epidemiological and -sociological patterns of herpes simplex virus infection in the world. | a newly developed herpes simplex virus (hsv) type 1 and type 2 enzyme immunoassay has been applied to over 40,000 sera from different populations in the us and 17 other countries on all 5 continents. the hsv-1 antibody patterns found permit the current and past socioeconomic status, as well as the extent of oral-genital sexual contact, to be deduced for various populations. the hsv-2 antibody results provide an objective measure of various aspects of sexual behavior: extent of polypartnerism in ... | 1990 | 2175939 |
| neurovirulence of herpes simplex virus types 1 and 2 isolates in diseases of the central nervous system. | herpes simplex virus (hsv) isolates derived from the central nervous system of ten patients with hsv-1-induced encephalitis, one patient with multiple sclerosis, and 14 patients with hsv-2-induced meningitis were investigated for neurovirulence by assaying the ld50 after nose and intracerebral (i.c.) inoculation of mice. hsv-1 encephalitis strains were significantly more virulent after nose inoculation (i.e. neuroinvasive) when compared with hsv-1 isolates from patients with oral lesions only, w ... | 1990 | 2175706 |
| arabinofuranosylpyrrolo[2,3-d]pyrimidines as potential agents for human cytomegalovirus infections. | protection of the 3'- and 5'-hydroxyl groups of the nucleoside antibiotic toyocamycin (1) with 1,3-dichloro-1,1,3,3-tetraisopropyldisiloxane was followed by (trifluoromethyl)sulfonylation of the 2'-hydroxyl group. a displacement of the resulting triflate ester moiety with lithium chloride, lithium bromide, sodium iodide, and lithium azide in hexamethylphosphoramide was followed by a removal of the disilyl moiety with tetra-n-butylammonium fluoride to afford the appropriate (2'-deoxy-2'-substitut ... | 1990 | 2175356 |
| effect of hexamethylene bisacetamide and cyclosporin a on recovery of herpes simplex virus type 2 from the in vitro model of latency in a human neuroblastoma cell line. | the goal of the present work was to examine whether hexamethylene bisacetamide (hmba) and cyclosporin a affect the recovery of herpes simplex virus type 2 (hsv-2) from an in vitro model of hsv-2 latency in human neuroblastoma cell line imr-32. imr-32 cells were infected with hsv-2 at a multiplicity of infection of 0.1 plaque-forming units/cell and were cultured at 40 degrees c for 14 days, resulting in the establishment of a model of hsv-2 latency in imr-32 cells. when the cultivation temperatur ... | 1990 | 2174735 |
| adenovirus infection in patients with kawasaki disease. | two outbreaks of kawasaki disease at different times and areas (kyoto in 1982 and sapporo in 1985) of japan were studied retrospectively for the presence of antibodies to adenoviruses and herpesviruses. only 2 of 12 (16.7%) consecutive acute phase sera of patients from the outbreak in 1982 and 1 of 10 (10.0%) sera from 1985 showed positive antibodies for the common adenovirus antigen by a complement fixation (cf) test, whereas 10 of 16 (62.5%) age- and sex-matched controls during the outbreak of ... | 1990 | 2173738 |
| mechanisms of inhibition of herpes simplex virus type 2 growth by 28-mer phosphorothioate oligodeoxycytidine. | the 28-mer phosphorothioate oligodeoxycytidine (s-(dc)28) has been reported previously to be a strong inhibitor of herpes simplex virus type 2 (hsv-2) dna polymerase and hsv-2 growth in cell culture. in this study, the mechanism of action of s-(dc)28 was studied. s-(dc)28 was found to interfere with the adsorption of hsv-1 and hsv-2 to hela cells. hsv-2 infection, but not hsv-1, was found to potentiate the uptake of s-(dc)28 into hela cells. the enhanced uptake reached a plateau at 6-9 h postinf ... | 1990 | 2173699 |
| viral etiology of chronic cervicitis and its therapeutic response to a recombinant interferon. | chronic cervicitis was shown to be related to papillomavirus type 16(hpv-16), herpes simplex virus type 2 and cytomegalovirus (cmv) infections as demonstrated by dna hybridization technique and virus isolation method from samples taken from erosive and normal cervices. after one course of treatment with recombinant interferon alpha 1 (rifn-alpha 1), 93.8% of cases showed clinical improvement and 60% marked improvement. the hpv-16 and hsv detection rates dropped down significantly after rifn-alph ... | 1990 | 2173655 |
| herpes simplex virus type 2 meningitis without genital lesions: an immunoblot study. | two sexually active female patients presented with acute meningitis. the csf abnormalities were severe and persistent. in spite of the absence of genital lesions, serological studies revealed a primary infection by herpes simplex virus type 2. an immunoblot study revealed intrathecal synthesis of anti-herpes antibodies early in the course of the disease. | 1990 | 2172470 |
| evolutionary relationships of virion glycoprotein genes in the s regions of alphaherpesvirus genomes. | the short region in the genome of herpes simplex virus type 1 contains a contiguous array of five genes (us4, us5, us6, us7 and us8) which encode known or proposed virion-surface glycoprotein species. counterparts for certain of these have been described in the genomes of other alphaherpesviruses, namely herpes simplex virus type 2, pseudorabies virus and varicella-zoster virus. within each of the us4-, us6- and us7-related sets, the amino acid sequences are most conserved in a region containing ... | 1990 | 2172448 |
| [the possibility of viral etiology in cervical carcinogenesis]. | oncogenic potential of herpes simplex virus type 2 (hsv-2) and human papillomavirus (hpv) which are both associated with occurrence of cervical cancer and the mechanism of oncogenesis by these viruses were investigated by transformation experiments in vitro. the results were obtained as follows. 1) hsv-2 induced neoplastic transformation of normal diploid cells is a multistep process. cervical cancer associated antigen ag-4 is encoded within the specific region of hsv-2 dna which converts immort ... | 1990 | 2172417 |
| use of polymerase chain reaction for successful identification of asymptomatic genital infection with herpes simplex virus in pregnant women at delivery. | the polymerase chain reaction was adapted to the amplification of a herpes simplex virus (hsv) dna sequence, common to hsv types 1 and 2 (hsv-1, hsv-2). the amplified product was detectable by ethidium-bromide staining or southern hybridization of gels and by dot hybridization. the hsv polymerase chain reaction detected hsv dna in samples obtained from eight patients with genital lesions from which hsv-2 was isolated in tissue culture and from four patients with labial lesions from which hsv-1 w ... | 1990 | 2172392 |
| herpes simplex virus genes controlling reactivation from latency in rabbit eye model. | using the rabbit eye model of latency, herpes simplex virus type 1 strain mckrae invariably reactivated after epinephrine iontophoresis, whereas type 2(hsv-2) virus strain hg 52 failed to reactivate. both strains established a latent infection with the same frequency. to identify the viral genes involved in this reactivation difference, intertypic recombinants were selected following cotransfection of intact mckrae dna and xba i or hpa i cleaved hg52 dna. eleven separately obtained recombinants ... | 1990 | 2172160 |
| the nucleotide sequence of the equine herpesvirus 4 gc gene homologue. | the genomic position of an equine herpesvirus 4 (ehv-4) gene homologue of the herpes simplex virus 1 (hsv-1) gc gene was determined by southern analysis and dna sequencing. the gene lies within a 2-kbp bg/ii-ecori fragment mapping between 0.15 and 0.17 within the long unique component of the ehv-4 genome and is transcribed from right to left. putative promoter elements were identified upstream of the 1455-bp open reading frame which encodes a 485-amino-acid protein of unglycosylated molecular we ... | 1990 | 2171212 |
| myristylation and polylysine-mediated activation of the protein kinase domain of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10). | the amino-terminal domain of the large subunit of herpes simplex virus type 2 (hsv-2) ribonucleotide reductase (icp10) was previously shown to possess protein kinase (pk) activity that localizes to the cytosolic, cytoskeletal, and plasma membrane fractions. further studies of the pk domain using computer-assisted sequence analysis have identified a single transmembrane segment and fatty acid incorporation findings indicate that icp10 is myristylated. myristylation does not depend on a viral enzy ... | 1990 | 2171204 |
| [guidelines for management of genital herpes infection in the experience of a health care center for sexually transmitted diseases in buenos aires (argentina)]. | during two years all clinical genital lesions compatible with herpes simplex virus (hsv) etiology were studied systematically through intent of virus isolation in bhk cells. isolation of virus was confirmed in 85% of the 206 cases studied. all seems to indicate that around the 20% of the isolations belong to the type hsv1 and the rest to the type hsv2. viral antigen demonstration, by direct and/or indirect technics with antibodies poly or monoclonals shows to be less sensitive than the virus iso ... | 1990 | 2170786 |
| characterization of herpes simplex virus type 2 transcription during latent infection of mouse trigeminal ganglia. | using a cornea trigeminal ganglion model, we have investigated transcription by herpes simplex virus type 2 (hsv-2) during latency in mice. latency was verified 2 months postinoculation by reactivation of hsv-2 after explant cocultivation of trigeminal ganglia from the majority of mice (83%). transcription during latent hsv-2 infection was limited to the repeat regions of the viral genome as determined by in situ hybridization using restriction fragment probes representing 100% of the hsv-2 geno ... | 1990 | 2170675 |
| [detection of herpes simplex virus, varicella zoster virus and cytomegalovirus in aphthous stomatitis]. | attempts were made to demonstrate herpes simplex virus (hsv) type 1 and type 2, varicella zoster virus (vzv) and cytomegalovirus (cmv) in specimens obtained from aphthous ulceration lesions by the immunofluorescent method using fluorescein-labeled monoclonal antibodies. hsv-1 and vzv were detected in 2 and 4 out of 30 patients, respectively. although almost all viruses that can infect the oral cavity could occasionally cause stomatitis, neither hsv-2 nor cmv was not found in this study. vzv was ... | 1990 | 2170607 |
| infection of vascular endothelial cells with herpes simplex virus enhances tissue factor activity and reduces thrombomodulin expression. | latent infection of vascular cells with herpes-viruses may play a pathogenic role in the development of human atherosclerosis. in a previous study, we found that cultured human umbilical vein endothelial cells (huvecs) infected with herpes simplex virus 1 (hsv-1) became procoagulant, exemplified both by their enhanced assembly of the prothrombinase complex and by their inability to reduce adhesion of platelets. we now report two further procoagulant consequences of endothelial hsv infection: los ... | 1990 | 2169619 |
| escherichia coli-derived envelope protein gd but not gc antigens of herpes simplex virus protect mice against a lethal challenge with hsv-1 and hsv-2. | immunization studies with hsv-1 and hsv-2 envelope proteins expressed in escherichia coli were performed. after active immunization of mice with a gd-1 antigen (leu53-ala312) expressed as a fusion protein, the animals were protected from a lethal challenge with hsv-1 and hsv-2. in addition, antisera from rabbits immunized with the same gd-1 antigen also conferred passive immunity to mice against a challenge infection with either hsv-1 or hsv-2. in contrast to these successful gd-1 protection exp ... | 1990 | 2169577 |
| [epidemiologic analysis of dna's of herpes simplex virus, type 1 and type 2 isolates obtained from family members and from different lesions of individuals]. | we analyzed herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) infections in 6 families, to see whether or not a single strain had infected members of each family, analyzing dna's of viral isolates with restriction endonucleases. we also analyzed 29 isolates from 13 patients to examine if herpetic lesions on different sites of each patient's body had been caused by a single strain. in 4 cases of family infection and 10 cases of individual infection with multiple site manifestations, electroph ... | 1990 | 2169506 |
| rapid detection of herpes simplex virus in clinical samples by flow cytometry after amplification in tissue culture. | murine monoclonal antibodies (mabs) against herpes simplex virus type 1 and 2 (hsv-1 and -2, respectively) nuclear antigens were used to identify cells infected with hsv-1 or -2 by indirect immunofluorescence in conjunction with flow cytometry after virus amplification of mrc-5 cell monolayers. the results indicate that mabs q1, q2, and h-640 detect hsv-1- and hsv-2-infected cells, mab sd-1 detects hsv-2- but not hsv-1-infected cells, and mab 58-s detects hsv-1- but not hsv-2-infected cells. mab ... | 1990 | 2168440 |
| papillomavirus trans-activator protein e2 activates expression from the promoter for the ribonucleotide reductase large subunit from herpes simplex virus type 2. | activation of the herpes simplex virus type 2 (hsv-2) large subunit of the ribonucleotide reductase (icp10) gene by papillomavirus dna encoding the e2 or e7 proteins was studied directly by immunofluorescence or by chloramphenicol acetyltransferase (cat) analysis with hybrid icp10 or ie175 and 38k promoter constructions. cotransfection with bovine papillomavirus type 1 or human papillomavirus type 16 (hpv-16) e2 dna enhanced cat expression from constructions in which cat is regulated by the icp1 ... | 1990 | 2167936 |
| high level expression in 293 cells of the herpes simplex virus type 2 ribonucleotide reductase subunit 2 using an adenovirus vector. | the herpes simplex viruses (hsv-1 and hsv-2) encode a ribonucleotide reductase consisting of two non-identical subunits (rr1 and rr2) which associate to form the active holoenzyme. to facilitate the purification and subsequent biochemical characterization of this enzyme, we have cloned the small subunit 2 of the hsv-2 ribonucleotide reductase (rr2hsv-2) in a helper-independent adenovirus type 5 vector under the control of the adenovirus type 2 major late promoter. after infection of 293 cells wi ... | 1990 | 2167932 |
| glycoprotein c-dependent attachment of herpes simplex virus to susceptible cells leading to productive infection. | herpes simplex viruses encode several glycoproteins dispensable for infection and replication in cell culture. evidence is presented that there exist at least two pathways for viral attachment to cells, i.e., one mediated by the dispensable glycoprotein c (gc) and one independent of that glycoprotein. thus, whereas the polycations neomycin and polylysine inhibit attachment but not entry of already attached herpes simplex virus 1 (hsv-1) into baby hamster kidney (bhk) cell line, they have no effe ... | 1990 | 2167550 |
| altered sensitivity to antiviral drugs of herpes simplex virus isolates from a patient with the acquired immunodeficiency syndrome. | acyclovir (acv)-resistant herpes simplex virus type 2 (hsv-2) was isolated from a patient with acquired immunodeficiency syndrome after long-term but intermittent acv therapy. these thymidine kinase-defective isolates were sensitive in vitro to foscarnet. while combined therapy with acv and interferon produced only partial clinical improvement, the in vitro effect of this combination against an acv-resistant isolate from the patient was strongly synergistic. a short course (10-12 days) of intrav ... | 1990 | 2167340 |
| reactivation of latent herpes simplex virus by adenovirus recombinants encoding mutant ie-0 gene products. | we have previously shown that adenovirus recombinants expressing functional icp0 reactivate latent herpes simplex virus type 2 (hsv-2) in an in vitro latency system. this study demonstrated that icp0, independent of other hsv gene products, is sufficient to reactivate latent hsv-2 in this in vitro system. to assess the effects of defined mutations in the sequence encoding icp0 (ie-0) on reactivation, seven in-frame insertion and three in-frame deletion mutants were moved into an adenovirus expre ... | 1990 | 2166826 |
| from virus to vaccine: recombinant mammalian cell lines as substrates for the production of herpes simplex virus vaccines. | the use of mammalian cells as substrates for the production of various vaccines is a time-honored procedure. with the advent of recombinant dna technology, various investigators soon realized that these techniques could be applied to mammalian cells to convert them to reliable, safe producers of a variety of vaccines. while none of these types of recombinant vaccines have, as yet, been widely utilized, many individuals believe that these technologies are the wave of the future in vaccine product ... | 1990 | 2165517 |
| alterations in the cellular phenotype induced by herpes simplex viruses. | numerous studies have shown that herpes simplex virus types 1 and 2 (hsv-1, hsv-2) are able to transform the morphological phenotype of rodent cells. unlike other dna tumor viruses the transformed cells did not consistently retain or express a given set of viral genes. in fact, transformation could be obtained using fragments of viral dna that did not wholly encode viral proteins. of interest within the transforming fragments were sequences which could assume a secondary structure like that of i ... | 1990 | 2165516 |
| the jh2604 deletion variant of herpes simplex virus type 2 (hg52) fails to produce necrotizing encephalitis following intracranial inoculation of mice. | the pathological changes and distribution of virus antigen in mouse brains were studied following intracranial inoculation of 3 week old balb/c mice with the herpes simplex virus (hsv) type 2 strain hg52 and its deletion variant jh2604. the variant jh2604 failed to produce necrotizing encephalitis compared to the parental hg52. the morphological changes induced in jh2604-infected brains consisted of localized perivascular cuffing by lymphocytes and infiltration by immune cells. immunohistochemic ... | 1990 | 2165139 |
| mucosal and systemic antiviral antibodies in mice inoculated intravaginally with herpes simplex virus type 2. | herpes simplex virus type 2 (hsv-2) causes lethal illness after intravaginal (ivag) inoculation into balb/cj mice. in the present studies, we demonstrated in mice that primary ivag vaccination with an attenuated strain of hsv-2 induced humoral immunity in sera and in vaginal secretions. secondary genital exposure to hsv-2 enhanced this response. however, intraperitoneal exposure to attenuated hsv-2 elicited an antiviral antibody response in sera but not in vaginal secretions. in both sera and va ... | 1990 | 2165134 |
| primary and recurrent herpes simplex virus type 2-induced meningitis. | in 27 patients with a first episode of herpes simplex virus type 2 (hsv-2)-induced meningitis, confirmed by virus isolation from the cerebrospinal fluid (csf) or seroconversion to hsv-2, initial neurologic complications were found in 10 (37%) but subsided before 6 months in all patients. long-term complications were recurrent meningitis in 5 (19%) and periodic headache related to genital hsv recrudescences in 4 (15%). seven additional patients had possible hsv-2-induced recurrent meningitis. in ... | 1990 | 2165105 |
| a cross-sectional study of herpes simplex virus types 1 and 2 in college students: occurrence and determinants of infection. | occurrence rates and determinants of infection with herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) were measured in first- and fourth-year undergraduate students at a state university. this cross-sectional multistage probability sample survey included sociodemographic characteristics, sexual behavior patterns, disease history, and hsv type-specific antibody status. the prevalence of hsv-1 antibody was 37.2% in freshmen and 46.1% in fourth-year students; that for hsv-2 antibody was 0.4% and ... | 1990 | 2165103 |
| epidemiology of genital herpes in pittsburgh: serologic, sexual, and racial correlates of apparent and inapparent herpes simplex infections. | women attending family planning clinics in western pennsylvania were enrolled into a prospective epidemiologic study of herpes simplex virus (hsv) infection. detection of antibodies to hsv-1 and hsv-2 was based on an immunodot assay using type-specific glycoproteins gg-1 and gg-2. serologic and historical data at enrollment were analyzed for 4527 subjects; the seroprevalence of hsv-2 was 21.6%. by multivariate analysis, hsv-2 infection as determined by seroprevalence was significantly and indepe ... | 1990 | 2165102 |
| nucleocapsid, nuclear association, and genome location of the herpes simplex virus type 2 38-kd dna-binding protein. | a previously-described herpes simplex virus type 2 dna-binding protein with a molecular size of 38 kd has been further characterized. using purified nucleocapsids, a dnase release assay, and intertypic recombinants, this protein was found to be a component of the nucleocapsid, intimately associated with the nuclear matrix, and encoded between 0.605 and 0.720 on the herpes simplex virus type 2 genome. | 1990 | 2165049 |
| immortalization of rabbit vascular smooth muscle cells after transfection with a fragment of the bglii n region of herpes simplex virus type 2 dna. | rabbit arterial smooth muscle cells were transfected with the pbc24neo plasmid dna which consists of a sequence of 790 base pairs from the bglii n fragment of herpes simplex virus type 2 dna linked to the neo resistance gene. selection in g418-containing medium resulted in eleven immortalized clones which showed increased growth rate and saturation densities. one of the eleven clones maintained the transforming dna sequence integrated in the cellular genome and showed continuous resistance to g4 ... | 1990 | 2165042 |
| a prospective evaluation of primary genital herpes simplex virus type 2 infections acquired during pregnancy. | in order to study the epidemiology of herpes simplex type 2 (hsv-2) infections during pregnancy, we used an enzyme immunoassay to detect type-specific antibodies to hsv-2 glycoprotein g in serial blood samples obtained from a cohort of 1891 pregnant women. blood samples obtained at about 17 and 32 weeks of gestation and at the time of delivery were assessed for antibody to hsv-2 glycoprotein g in order to evaluate the prevalence of past infections with hsv-2 and the rate of acquisition of hsv-2 ... | 1990 | 2164656 |
| serum amyloid a protein-related mrna expression in herpes simplex virus type 2-transformed hamster cells. | a nearly full-length cdna very similar to murine serum amyloid a protein was cloned from herpes simplex virus type 2-transformed hamster cells. the expression of mrna was constitutive in these cells and could be superinduced by conditioned medium. higher rates of nuclear runoff transcription in transformed and induced cells indicated some regulation at the transcriptional level. | 1990 | 2164641 |
| serologic type conversion of a herpes simplex virus type 1 (hsv-1) to an hsv-2 epitope caused by a single amino acid substitution in glycoprotein c. | a monoclonal antibody to herpes simplex virus type 2 glycoprotein c (gc-2) did not recognize wild-type herpes simplex virus type 1 gc (gc-1) but did recognize a mutant gc-1 molecule. this conversion from a type 1 to a type 2 epitope was shown to be due to a single amino acid substitution in gc-1. | 1990 | 2164613 |
| identification of the cross-reactive antigens between marek's disease virus and pseudorabies virus by monoclonal antibodies. | among the 33 monoclonal antibodies (mabs) against pseudorabies virus (prv) examined, three mabs (24-17, 74-26, and 8) were found to react with cells infected with marek's disease virus (mdv)-related viruses by immunofluorescence test. two of the mabs (24-17 and 74-26) reacted with the nuclei of cells infected with mdv serotype 1 (mdv1), mdv serotype 2 (mdv2), and herpesvirus of turkeys (hvt), whereas mab 8 reacted with the cytoplasm of mdv2- and hvt-infected cells. however, none of the mabs agai ... | 1990 | 2164393 |
| genomic variation of herpes simplex virus type 2 isolates analysed by hybridization after electroblotting from polyacrylamide gels. | genomic variation of herpes simplex virus type 2 (hsv-2) strains was analysed by polyacrylamide gradient gel electrophoresis and subsequent hybridization to cloned hsv-2 sequences. two probes were used, one from the l-segment and one from the s-segment of the hsv-2 genome. the probes did not contain a-repeat sequences. hybridization to the specific sequences in individual dna fragments obtained by use of the frequently cleaving restriction endonuclease alu i revealed variations in the genome not ... | 1990 | 2164145 |
| no association between herpes simplex virus type-2 seropositivity or anogenital lesions and hiv seroconversion among homosexual men. | recent reports have suggested that hsv-2 infection and associated anogenital ulcerations represent an important risk factor for acquisition of hiv infection. although this is an appealing biological hypothesis, inferences drawn for homosexual men, as well as other at-risk populations, must be made after careful consideration of methods to control for potential confounding data. this report utilized a nested case-control study in which 49 homosexual hiv seroconverters were compared to 49 homosexu ... | 1990 | 2164082 |