Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| a major histocompatibility locus in fish: serological identification and segregation of transplantation antigens in the common carp (cyprinus carpio l.). | in this study, experiments are described that were designed to investigate whether fish have an immune regulatory systems similar to the major histocompatibility complex (mhc) in higher vertebrate species. from combinations of gynogenetic carp showing either slow or fast rejection of skin transplants, the latter were chosen for alloantiserum production by hyperimmunization with peripheral blood leucocytes. the resulting alloantisera were analyzed for hemagglutinating reactivity with gynogenetic ... | 1989 | 2793208 |
| [the dynamics of the immunologic indices of carp exposed to methylene blue]. | several immunological and haematological indices as well as infection of carp with ectoparasites of the dactylogyrus genus have been studied in fish that have been subjected to methylene blue treatment, to infection with rhabdovirus carpio and aeromonas hydrophila in different combinations. a dosage of 0.05 mg/l (exposure lasting for 3 days) over 20 days increases most of the immunological indices. a treatment of the carp with drug (dosage of 100 mg/l) for 4 hours has a stimulating effect on the ... | 1989 | 2792442 |
| mechanism of the water response in frog gustation: possible significance of surface potential. | the frog taste response to deionized water (water response) after adaptation of the tongue to salts was recorded from the glossopharyngeal nerve under various conditions. it was found that the frog water response exhibits different behavior from the carp water response examined in a previous paper13. (a) the frog water response did not decline during stimulation and lasted for at least 3 min, while the carp water response declined within 10 s after stimulation to a spontaneous level. (b) the fro ... | 2009 | 2786441 |
| [the infection process with sphaerospora renicola (myxosporidia)]. | new data are presented on the development of infection by s. renicola in common carp within the first 15 weeks of their life. the infection of parasite-free common carp succeeded by myxospores as well as by feeding carp kidneys with and without spores in laboratory experiments. in field experiments one year-old parasite-free common carp were successfully infected. | 1989 | 2782666 |
| restrained least squares refinement of native (calcium) and cadmium-substituted carp parvalbumin using x-ray crystallographic data at 1.6-a resolution. | carp parvalbumin coordinates calcium through one carbonyl oxygen atom and the oxygen-containing side chains of 5 amino acid residues, or 4 residues and a water molecule, in a helix-loop-helix structural motif. other calcium-binding proteins, including calmodulin and troponin c, also possess this unique calcium-binding design, which is designated ef-hand or calmodulin fold. parvalbumin has two such sites, labeled cd and ef. each of the calcium-binding sites of refined structures of proteins belon ... | 1989 | 2777802 |
| enzymatic properties of glyceraldehyde-3-phosphate dehydrogenase from fish muscle. | 1. glyceraldehyde-3-phosphate dehydrogenase was isolated from the ordinary muscle of red sea bream pagrus major, pacific mackerel scomber japonicus and carp cyprinus carpio by ammonium sulfate fractionation, followed by deae-sepharose cl-6b and deae-cellulose column chromatography and sephadex g-150 gel filtration, and examined for enzymatic properties. 2. their optimum ph values in the backward reaction ranged from 7.8 to 8.2, and km values from 1.56 to 1.90 mm. 3. irrespective of the species o ... | 1989 | 2776431 |
| an effective method for plasma lipoprotein separation: studies of various animal species. | 1. plasma lipoprotein separation by density gradient ultracentrifugation largely depends on visual examination based on the natural yellow pigments of lipoproteins. 2. in non-human species and in humans with dyslipoproteinemia, some lipoproteins are not well visualized due to the lack of pigments. 3. using a fluorescent probe in minute quantity (1,6 diphenyl-1,3,5-hexatriene) we were able to demonstrate an effective plasma lipoprotein separation using a discontinuous density gradient ultracentri ... | 1989 | 2776426 |
| identification and determination of tert-alkylphenols in carp from the trenton channel of the detroit river, michigan, usa. | whole carp from the detroit river were analyzed by gas chromatographic mass spectrometry. seven tertiary alkyl phenols (tert-pentylphenols, tert-butyl-tert-pentylphenols, a di-tert-pentylphenol and a tri-tert-pentylphenol) and eight chlorinated derivatives of these compounds were identified from their mass spectra and confirmed with synthesized authentic standards. 2,4-di-tert-pentylphenol was the most abundant of these compounds; in one fish sample, its concentration was about 140 ppm. the same ... | 1989 | 2775903 |
| an homologous radioimmunoassay for coho salmon (oncorhynchus kisutch) vitellogenin, with general applicability to other pacific salmonids. | this paper describes an homologous radioimmunoassay for coho salmon vitellogenin that demonstrates parallel cross-reactivity for plasma vitellogenin of all pacific salmonids tested (chinook, chum, coho, pink, and sockeye salmon, and cutthroat and rainbow trout), but not for atlantic salmon or two nonsalmonids: common carp and sablefish. plasma vitellogenin levels were high in ovulatory female pacific salmonids (micrograms/ml to mg/ml range), but were mostly nondetectable in spermiating males of ... | 1989 | 2767411 |
| immunostimulating influence of levamisole on nonspecific immunity in carp (cyprinus carpio). | 1989 | 2767313 | |
| the beta-actin gene of carp (ctenopharyngodon idella). | 1989 | 2762162 | |
| an immunological study of the secretory activity of neurons producing melanin-concentrating hormone in a teleost. | the melanin-concentrating hormone is a general vertebrate neurosecretory peptide which, in bony fish, serves as a neurohypophysial hormone influencing pigmentary changes in response to background colour. young carp were reared for six months in white- or black-coloured tanks to determine how this would influence the development of the neurons producing the peptide. cytological criteria and radioimmunoassay of tissue extracts showed that the background markedly influenced the synthetic activity o ... | 2008 | 2761693 |
| development of trypanoplasma borreli (mastigophora: kinetoplastida) in the leech vector piscicola geometra and its infectivity for the common carp, cyprinus carpio. | the development of trypanoplasma borreli in the crop of the leech vector piscicola geometra was characterized by significant changes in morphology. immediately after ingestion by the leech, stumpy-shaped t. borreli predominated and numerous dividing specimens were found. this led to long and slender trypanoplasms near the end of the infection. the infection was terminated with complete digestion of the blood stored in the crop of the leech. the longest period of infection observed was 11 days. t ... | 1989 | 2760765 |
| comparative study of the anatomy and laminar organization in the olfactory bulb of three orders of freshwater teleosts. | the anatomy and lamination of the olfactory bulb in cyprinus carpio, tinca tinca, barbus bocagei (fam. cyprinidae, or. cypriniformes); salmo gairdneri (fam. salmonidae, or. salmoniformes); and gambusia affinis (fam. poeciliidae, or. cyprinodontiformes), all of them freshwater teleosts, are studied. these species show significative differences on the location, size, morphology, and lamination of their olfactory bulbs. the presence of a new stratum in the olfactory bulb of salmo gairdneri and a co ... | 1989 | 2759410 |
| [opisthorchiasis at left dnieper watersheds]. | present state of opisthorchosis infestation in the dnieper watershed (rivers vorskla, psyol, khorol, sula, seym, snov, desna water basins), situated in the sumy, chernigov, and poltava regions of the ukraine, is assessed. 7909 humans from 37 villages, 94 cats, 1134 carp fishes, 20013 mollusca and 30 soil samples were screened for the presence of opisthorchis eggs. opisthorchosis foci with an over 36% infection rate of population remain in the watersheds of the dnieper left confluents (rivers des ... | 1989 | 2755409 |
| carp growth hormone: molecular cloning and sequencing of cdna. | cdna clones of the fish cyprinus carpio growth hormone (gh) mrna have been isolated from a cdna library prepared from carp pituitary gland poly(a)+rna. the nucleotide sequence of one of the carp gh cdna clones containing an insert of 1164 nucleotides (nt) was determined. the cdna sequence was found to encode a polypeptide of 210 amino acids (aa) including a signal peptide of 22 aa and to contain 5' and 3' untranslated regions of the mrna of 36 and 498 nt, respectively. the carp gh presents a 63% ... | 2008 | 2753359 |
| effects of anoxia on catecholamine levels in brain and kidney of the crucian carp. | catecholamine synthesis requires o2. crucian carp (carassius carassius l.), which are extremely anoxia tolerant, were exposed to anoxia for 76 or 160 h. the brain levels of dopamine and norepinephrine (no epinephrine was found in brain) remained relatively constant even after nearly 1 wk of anoxia, indicating very well-functioning transmitter reuptake mechanisms and/or the absence of o2-independent degradation. in contrast, in the kidney (which contains chromaffin tissue), the catecholamine cont ... | 1989 | 2750953 |
| dendritic morphology of retinal dopamine cells in carp of different sizes. | the dendritic morphology of dopamine (da) cells in the inner plexiform layer of the retina of different-sized carp (8.6-33.6 cm in body length) was investigated by identifying their fluorescent cell bodies in isolated, aldehyde-fixed flatmounts and injecting them iontophoretically with lucifer yellow (ly) under microscopic control. da cells for ly injection were chosen in an intermediate region between the optic disc and the retinal margin, and their spatial parameters (cell density, nearest nei ... | 2008 | 2743560 |
| interactions between oocytes of different maturational stages in the carp cyprinus carpio: effects on maturational and steroidogenic activity in vitro. | ovarian fragments from carp which had received injections of saline (control) or carp hypophysial homogenate (primed) were incubated both alone and a cocultures of primed and control tissue in the presence of carp pituitary extract. the results showed that the cocultures were not simply the sums of separate incubations. oocyte maturation was retarded in primed tissue but advanced in control tissue and concentrations of 17,20 beta-dihydroxy-4-pregnen-3-one (17,20 beta p) in the medium were signif ... | 1989 | 2737454 |
| characterization of gamma-crystallins from eye lenses of shark: closer structural similarity to mammalian than other piscine gamma-crystallins? | lens crystallins were isolated and characterized from sharks of the cartilaginous fishes. four crystallin fractions corresponding to alpha-, beta h-, beta l- and gamma-crystallins, similar to those of mammalian crystallins, were obtained. the native molecular masses and subunit structures of these purified fractions were analyzed by gel permeation chromatography, sds gel electrophoresis and isoelectric focusing, revealing the typical subunit compositions with various extents of heterogeneity in ... | 1989 | 2737298 |
| effects of metal ions on cyprinid fish immune response: in vitro effects of zn2+ and mn2+ on the mitogenic response of carp pronephros lymphocytes. | lymphocytes from the pronephros of carp (cyprinus carpio l) have been subjected to transformation by mitogens, concanavalin a (con a), phytohemagglutinin (pha), and lipopolysaccharides (lps), with zn or mn at varying concentrations. addition of zn2+ (10(-7) to 10(-3) m) to mitogen-stimulated t and b cells enhanced [3h]thymidine incorporation. addition of 10(-5) m zn2+ inhibited the response to con a, pha, and lps. at this concentration, zn was toxic. addition of mn2+ (10(-7) to 10(-3) m) to mito ... | 1989 | 2737112 |
| 1,1,1-trichloroethane: medium-term toxicity to carp, daphnids, and higher plants. | the toxicity of 1,1,1-trichloroethane to carp (cyprinus carpio) and daphnids (daphnia magna) and the effects of gaseous-phase exposure on the growth of higher plants (sorghum bicolor and brassica napus) were investigated. the test systems were designed to minimize loss of the volatile material during the exposure period. during an exposure period of 14 days, there were no mortalities or other symptoms of toxicity in carp exposed to a mean measured 1,1,1-trichloroethane concentration of 7.7 mg/li ... | 1989 | 2737111 |
| natural environment surpasses polluted environment in inducing dna damage in fish. | measurement of specific dna adduct concentrations in target tissues of organisms may provide a key biologic end-point of exposure to environmental carcinogens. using a general and highly sensitive assay with 32-p-postlabeling, we found that natural populations of freshwater fish species chub, barbel, bream and carp, as well as a marine fish mugil, revealed the presence of four to nine qualitatively similar adducts irrespective of whether they were caught from unpolluted or polluted waters. no st ... | 1989 | 2736723 |
| lateral feedback from monophasic horizontal cells to cones in carp retina. ii. a quantitative model. | about half of the monophasic horizontal cells in carp retina receive input from both red- and green-sensitive cones. since the horizontal cells feed back to cones, the color and feedback pathways result in wavelength- and intensity-dependent changes of the dynamics and of the receptive field amplitude profile of the horizontal cell responses. in this paper we present a quantitative model that describes adequately the color and spatial coding and the dynamics of the responses from monophasic hori ... | 1989 | 2732680 |
| lateral feedback from monophasic horizontal cells to cones in carp retina. i. experiments. | the spatial and color coding of the monophasic horizontal cells were studied in light- and dark-adapted retinae. slit displacement experiments revealed differences in integration area for the different cone inputs of the monophasic horizontal cells. the integration area measured with a 670-nm stimulus was larger than that measured with a 570-nm stimulus. experiments in which the diameter of the test spot was varied, however, revealed at high stimulus intensities a larger summation area for 520-n ... | 1989 | 2732679 |
| oxidative properties of carp red and white muscle. | substrate preferences of isolated mitochondria and maximal enzyme activities were used to assess the oxidative capacities of red muscle (rm) and white muscle (wm) of carp (cyprinus carpio). a 14-fold higher activity of citrate synthase (cs) in rm reflects the higher mitochondrial density in this tissue. rm mitochondria oxidize pyruvate and fatty acyl carnitines (8:o, 12:o, 16:o) at similarly high rates. wm mitochondria oxidize these fatty acyl carnitines at 35-70% the rate of pyruvate, depending ... | 1989 | 2732662 |
| hydrogen ion equilibria in fish haemoglobins. | h+ titration studies on oxygenated and deoxygenated haemoglobins from carp, rainbow trout, spiny dogfish and pig are reported, and compared with hb-h+ equilibria in other species and structural information deduced from amino acid sequences. the buffer values of oxygenated and deoxygenated teleost haemoglobins are low in comparison with elasmobranch and mammalian haemoglobins. this correlates with a much lower content of histidine residues and alpha-amino groups in teleost haemoglobins, than that ... | 1989 | 2732661 |
| [seasonal dynamics of the population count of bothriocephalus opsariichthydis (cestoda, pseudophyllidea) and the differential infestation of carp yearlings of various genotypes]. | 1989 | 2717201 | |
| [lipid composition of cyprinidae species. 1. lipid content and fatty acid pattern of silver carp (hypophthalmichthys molitris (val.))]. | 1989 | 2716812 | |
| efferent tectal cells of crucian carp: physiology and morphology. | tectal cells of the crucian carp (carassius ararssius) showing antidromic responses evoked by rhombencephalic electrical stimulation were physiologically studied and subsequently stained with lucifer yellow ch. the stained efferent tectal cells were fusiform, horizontal, and multipolar. the main axon of these efferent tectal cells descended along the wall of the deep tegmentum and could be traced to the motor area below the cerebellum. the axons gave off their collaterals in several brain areas: ... | 1989 | 2706544 |
| amino acid sequence of alpha-chain of hemoglobin iv from trout (salmo irideus). | the amino acid sequence of the alpha-chain of trout hemoglobin (hb) iv is given, thus completing the primary structure of the hemoglobin component of trout's blood characterized by the root effect. the trout hb iv alpha-chain consists of 142 amino acid residues; comparison with the corresponding sequences from human and carp hemoglobins shows differences of 50.0 and 35.9%, respectively. a difference of 39.6% is found with the alpha-chain of trout hb i, the other major hemoglobin component of tro ... | 1989 | 2706274 |
| fish muscle energy metabolism measured by in vivo 31p-nmr during anoxia and recovery. | by means of in vivo 31p nuclear magnetic resonance (nmr) we measured energy stores and intracellular ph at 10-min intervals in the myotome of unanesthetized carp and goldfish before, during, and after a period of anoxia (1 h for carp and 4 h for goldfish). the fish were mounted in a modified bioprobe, and their gills were irrigated with a constant flow of aerated or anoxic water. anoxia caused a steep decline of phosphocreatine and intracellular ph in carp muscle. after the phosphocreatine store ... | 2009 | 2705580 |
| [new data on some parasitic invasions in fish]. | the author gives a review of new literature data from the 80-ties. the following problems are considered: etiology of swim bladder inflammation in carp (sbi), development of parasites of the class myxosporea, overgrowth of parasitic kidney disease (pkd) in salmonid fishes, details of development of myxobolus cerebralis and ichthyophtirius multifiliis, and the new systematics of the diplozoidae. | 1989 | 2699131 |
| effect of a teleost gnrh analog on steroidogenesis by the follicle-enclosed goldfish oocytes, in vitro. | the influence of an agonist analog of teleost gnrh [(d-arg6, trp7, leu8, pro9-net)-gnrh; tgnrh-a] on steroidogenesis was studied in prophase-i arrested, follicle-enclosed, goldfish oocytes in vitro. incubation of the follicles with carp gonadotropin (gth) significantly increased production of 17 alpha-hydroxyprogesterone (hp) and testosterone following 24 hr of incubation in vitro. concomitant incubation with tgnrh-a (10(-7) m) significantly attenuated the dose-related increase in gth-induced te ... | 2009 | 2689273 |
| development of an enzyme-linked immunosorbent assay for goldfish gonadotropin. | an enzyme-linked immunosorbent assay (elisa) for goldfish gonadotropin (gth) was developed with the intent of devising a simple, reliable and nonradioisotopic assay for the measurement of gth in goldfish biological samples. in this assay, soluble gth of the standards or samples competes with carp gth (cgth) immobilized on a solid support (96-well microplate) for the fixation on antibodies to the beta-subunit of carp gonadotropin. the immobilized antigen-antibody complexes are then revealed by th ... | 1989 | 2679899 |
| immunohistochemical characterization of lymphocyte populations in carp (cyprinus carpio l.). | surface immunoglobulin-bearing cells from peripheral blood, thymus, head kidney, and spleen in carp (cyprinus carpio l.) were studied by immunoperoxidase labelling using antisera against fragments of carp igm. different lymphoid cell populations could be determined. thus, about 35% of the lymphocytes of the peripheral blood, head kidney, and spleen were determined, as "b" lymphocytes, using rabbit anti-carp fab antiserum with l chain specificity. nearly all lymphocytes of the peripheral blood, h ... | 1989 | 2673224 |
| new trends in parasitic infections of cultured freshwater fish. | the 1980s are characterized by the appearance of new fish parasitoses, the clarification of the aetiology of diseases and new insights into the process of infection and the development of important parasites, especially myxosporidians. two phaerosporoses have entered into the foreground: swimbladder inflammation (sbi) caused by sphaerospora renicola in common carp and proliferative kidney disease (pkd) in salmonids. both diseases must be reported in the german democratic republic (gdr). the conn ... | 1989 | 2669320 |
| immunocytochemical demonstration of melanin-concentrating hormone and proopiomelanocortin-like products in the brain of the trout and carp. | immunocytochemistry on frozen sections revealed that in both the trout and the carp, parvocellular neurones located in the medial basal hypothalamus (medial nucleus lateralis tuberis) were immunostained by antisera against three molecules known to be derived from the proopiomelanocortin (pomc) molecule, viz: alpha-melanocyte-stimulating hormone (alpha msh), acth, and salmonid npp--the whole n-terminal sequence preceding acth in the pomc precursor. axons from these neurones extended into various ... | 1989 | 2663629 |
| bacterial action of fertilization envelope extract from eggs of the fish cyprinus carpio and plecoglossus altivelis. | the vitelline envelope (ve) and fertilization envelope (fe) in eggs of the fish cyprinus carpio and plecoglossus altivelis were purified by homogenization of eggs or embryos in 5 mm tris-hcl buffer, ph 7.0, containing 2 mm ethylenediamine tetraacetic acid disodium salt (edta), except for processing of ves in plecoglossus eggs, and by repeated washing wih the same buffer. to extract the outermost layer material, the purified ves and fes were processed overnight at 4 degrees c in 5 mm tris-hcl buf ... | 1989 | 2661718 |
| effects of textile dye stuff effluent on food utilization of cyprinus carpio (linn.). | dye stuff effluent at 15% caused 100% mortality within 24 hr; 96 hr lc50 value being 10% concentration. prolonged exposure of c. carpio to chosen sublethal concentrations (1, 3, 5, 7 or 9%) reduced the food intake and increased the maintenance cost. exposure also caused significant reduction in growth rate and conversion efficiency; these values averaged to 11 mg/g/day and 28% in the control group (0% concentration) respectively and decreased to 0.8 mg/g/day and 4% respectively at 9% concentrati ... | 1989 | 2633962 |
| in vitro effect of mammalian pituitary hormones on germinal vesicle breakdown in oocytes of major carps, labeo rohita, cirrhinus mrigala, catla catla and cyprinus carpio. | among all the mammalian pituitary hormones, luteinizing hormone (lh) was the most potent in vitro inducer of oocyte maturation in l. rohita, c. mrigala, c. catla and c. carpio. it induced significant germinal vesicle breakdown (gvbd) at concentrations of 10, 1, 0.1 and 0.01 micrograms/ml. at the highest concentration used, lh induced 77.9 +/- 5.9, 73.8 +/- 4.6, 50.3 +/- 2.8 and 40.8 +/- 1.4% gvbd in oocytes of l. rohita, c. mrigala, c. catla and c. carpio, respectively. among other hormones, fol ... | 1989 | 2632393 |
| fast activity and oscillatory potential of carp retina in the frequency domain. | there are two kinds of fast activity in the erg: fast retinal potentials (frp), an irregular series of spiky wavelets and oscillatory potentials (op), a rhythmic sequence of events. corneal erg from nine intact young carps, evoked by extended pulses of diffuse white light under mesopic adaptation, displayed two different groups of wavelets related to on and off, respectively. stimulation and recording conditions were established to permit separate fourier analysis of both groups of wavelets. pow ... | 1989 | 2629209 |
| ultrastructural observations on different developmental stages of goussia sinensis (chen, 1955), a parasite of the silver carp (hypophthalmichthys molitrix valenciennes, 1844). | the development of goussia sinensis, a coccidium parasitizing the intestine of the silver carp (hypophthalmichthys molitrix) was studied by electron microscopy. all stages developed in the epithelial cells, less frequently in the goblet cells, and were located within a parasitophorous vacuole. in some cases one cell was invaded by several merozoites. eight to sixteen merozoites were formed within the meront by ectomerogony. the ultrastructural processes characteristic of gamogony were the same a ... | 1989 | 2627006 |
| [effects of uv-irradiation of carp sperm and its modification by caffeine]. | in connection with the development of uv-induced mutagenesis in carp, the effects of uv-irradiation of sperms have been studied in the range of doses 0.3-40 j/m2. the irradiation did not cause reduced fertilization ability and cleavage delay. small doses of irradiation (0.6 j/m2) produced stimulating effect on embryo survival, the larvae yield decreasing and the amount of aberrant anaphases increasing, as the doses are increased. ld50 is 6.0 j/m2 for embryonal period of carp development, photore ... | 1989 | 2625201 |
| aldehyde dehydrogenase activity in blood from various vertebrates. | 1. aldehyde dehydrogenase activity was determined in whole blood samples from 17 selected vertebrates of 5 classes, using 3,4-dihydroxyphenylacetaldehyde (the aldehyde derived from dopamine) as substrate. 2. aldehyde dehydrogenase activity in blood was widely but unevenly distributed among the species studied. 3. mean aldehyde dehydrogenase activities in the range of 40-140 nmol/min.ml blood (measured at 37 degrees c, ph 8.8) were found in blood from man, monkey, rabbit, guinea pig and mouse (c5 ... | 1989 | 2620490 |
| neural mechanisms of chromatic adaptation in l-type cone horizontal cells of the carp retina. | when a background light is delivered, the responsiveness of horizontal cells to light stimulus initially lowers but subsequently recovers gradually as shown by the increase in response amplitude to test light. these changes of responsiveness are observed when white or close wavelengths are used for both the background and test lights. however, the response amplitude to blue-green test light was initially enhanced but decreased and reached a steady state after the onset of red background illumina ... | 1989 | 2615034 |
| host-parasite interface of paratenuisentis ambiguus (eoacanthocephala) in naturally infected eel and in laboratory-infected sticklebacks and juvenile carp and rainbow trout. | paratenuisentis ambiguus is described from natural infections in adult eels and from laboratory infections in sticklebacks and juvenile carp and rainbow trout. in captured eels, female worms kept reproducing in the laboratory for at least 1 mo. in the 3 small laboratory hosts female worms did not release eggs and longevity did not exceed 3-30 days. it is concluded that the small fishes were unsuitable final hosts. worm penetration into the intestinal wall of all hosts was shallow. thus, the smal ... | 2008 | 2614601 |
| cell proliferation in the olfactory bulb of adult freshwater teleosts. | the olfactory bulb of adult specimens of carassius auratus was subjected to an autoradiographic study using [3h]thymidine. adult specimens of carassius auratus, barbus meridionalis, cyprinus carpio and salmo gairdneri were also studied using light and electron microscopy. cells were found undergoing mitotic division with two characteristic locations: a group can be seen near to the ventricle or ventricular recess; these cells can be identified as granule cells or precursors of granule cells. oth ... | 2008 | 2606770 |
| morphology of blood cells in carp (cyprinus carpio l.). | the morphology of blood cells in the carp was investigated by light and electron microscopy. erythrocytes, thrombocytes, lymphocytes, granulocytes and monocytes were identified as the peripheral blood cells. thrombocytes were round to long oval, each containing vesicular and microtubular structures and an oval nucleus with abundant heterochromatins. lymphocytes were divided into three types in size, small, medium and large. some of the small and medium lymphocytes were alpha-naphthyl-acetate est ... | 1989 | 2601228 |
| motor innervation of respiratory muscles and an opercular display muscle in siamese fighting fish betta splendens. | horseradish peroxidase was used to identify motor neurons projecting to the adductor mandibulae, levator hyomandibulae, levator operculi, adductor operculi, and dilator operculi muscles in siamese fighting fish, betta splendens. these muscles participate in the production of respiratory and feeding movements in teleost fishes. the dilator operculi is also the effector muscle for gill-cover erection behavior that is part of betta's aggressive display. the motor innervation of these muscles in bet ... | 1989 | 2592620 |
| purification and characterization of cu,zn-superoxide dismutase from common carp liver. | 1. common carp (cyprinus carpio l.) liver cu,zn-superoxide dismutase (cu,zn-sod) was purified and characterized. 2. its molecular weight, isoelectric point, electrophoretic mobility, amino acid pattern and some other characteristics were determined. | 1989 | 2591201 |
| comparison of xanthine: nad+ oxidoreductase from liver of toad bufo viridis and other vertebrates. | 1. xanthine oxidoreductase was isolated from toad bufo viridis (a mainly ureotelic amphibian species) and partially purified. the enzyme occurred as a stable xanthine: nad+ oxidoreductase (ec 1.1.1.204), unconvertible to the oxidase form. 2. some properties of the enzyme resembled those of xanthine oxidoreductase from an ammonotelic fish, cyprinus carpio, and the ureotelic rat, but in other aspects it was similar to this enzyme from an uricotelic snake, natrix natrix. 3. inhibition of the toad e ... | 1989 | 2591196 |
| biological activities of ganglion extracts from a prosobranch mollusc, fusinus ferrugineus. | acetone extract of the ganglia of fusinus ferrugineus was applied to c-18 cartridges, and the flowthrough and retained materials were bioassayed on the radula retractor muscle of the animal. the flowthrough was found to have a potent contractile action and the retained material a potent contraction-inhibiting action in addition to a weak contractile action. the retained material was then applied to a column (2.6 x 40 cm) of sephadex g-15. fractions of 4 ml each were collected, and their activiti ... | 1989 | 2584053 |
| adenohypophysial cell types in an indian freshwater major carp, catla catla (hamilton). | the different adenocytes in an indian freshwater major carp, catla catla (hamilton) have been studied by employing various modern staining techniques. altogether, 8 different cell types have been reported in the adenohypophysis of the aforesaid fish. variations in the staining intensities of the cell types with the different techniques adopted have been detailed. | 1985 | 2581848 |
| phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding. | of eight monoclonal antibodies raised against human transferrin, one (h.tf-14) cross reacted with pig and rabbit transferrins and one (h.tf-1) showed cross-reactivity with horse and dog transferrins. while rabbit and pig transferrins exhibited the same patterns of binding to molt-3 cell receptors as human and horse transferrins, binding of mouse and dog transferrins was weaker and bovine and carp transferrins gave entirely negative results. the results of these competitive binding experiments we ... | 1985 | 2578807 |
| production of monoclonal antibodies directed against antigenic determinants common to the alpha- and beta-chain of bovine brain s-100 protein. | balb/c mice were immunized with s-100 protein, which was isolated from bovine brain. the first fusion resulted in the cloning of three stable hybridoma lines (s1-61-64, s1-61-65, s1-87-4) that produced monoclonal antibodies against s-100 protein. the hybridoma lines obtained from a second fusion (s2-20, s2-95) were not stable and antibody production ceased early during cloning. immunoblotting results showed that all antibodies reacted with antigenic determinants shared by both the alpha- and bet ... | 1985 | 2578587 |
| d-aspartate potentiates the effects of both l-aspartate and l-glutamate on carp horizontal cells. | l-aspartate, l-glutamate and d-aspartate, alone or in combination, were applied by superfusion or by atomization to the isolated carp retina while recording from cone horizontal cells. each of these agents when applied alone depolarized horizontal cells and reduced the size of their light responses, an action which mimics the effect of the endogenous photoreceptor transmitter. application of d-aspartate in conjunction with either of the l-amino acids potentiated the effects the l-amino acids so ... | 1989 | 2573859 |
| the roles of alpha- and beta-adrenoceptors in the chronotropic responses to norepinephrine in carp heart (cyprinus carpio). | 1. the chronotropic effect of norepinephrine was studied in isolated spontaneously beating atrial preparations of carp (cyprinus carpio) heart. 2. norepinephrine, 0.1 microm, caused a positive chronotropic effect, while at 1 microm it caused either a positive or a negative chronotropic effect. the positive chronotropic effect, observed in 13 preparations, was potentiated by phentolamine and almost completely blocked by propranolol. 3. the negative chronotropic effect observed in the other 5 prep ... | 1989 | 2566437 |
| histamine directly acts on beta-adrenoceptors as well as h1-histaminergic receptors, and causes positive inotropic effects in isolated ventricular muscles of carp heart (cyprinus carpio). | 1. the mechanism for positive and negative inotropic effects of histamine was studied in electrically stimulated ventricular strips of carp heart. 2. a high concentration of histamine (1 mm) caused a transient negative, and subsequent positive inotropic effects. the positive effect was significantly reduced by pyrilamine, diphenhydramine or dl-propranolol, but was not affected by cimetidine or d-propranolol. 3. prior treatment with reserpine significantly decreased epinephrine and norepinephrine ... | 1989 | 2566436 |
| some characteristics of mitochondrial monoamine oxidase activity in eggs of carp (cyprinus carpio) and rainbow trout (salmo gairdneri). | 1. monoamine oxidase (mao) activity towards tryptamine, 5-hydroxytryptamine (5-ht) and phenylethylamine (pea) has been measured in mitochondria isolated from carp and trout eggs. 2. in carp eggs all the tested substrates are metabolized and the highest affinity is found with tryptamine. in trout eggs a consistent level of mao activity is obtained using tryptamine. 3. the inhibition dose-response curves of clorgyline and deprenyl indicate that both in carp and trout eggs there is only one form of ... | 1989 | 2565193 |
| glucuronides in mussel mytilus galloprovincialis as a possible biomonitor of environmental carcinogens. | 1. the in vitro incubation of mussel digestive gland with 1 mm aminofluorene resulted in the formation of glucuronides that (a) became mutagenic with carp liver s9, and (b) liberated s9-dependent mutagenic aglucones after beta-glucuronidase treatment. 2. natural populations of mussels from unpolluted and polluted sites, as well as mussels exposed to 3 ppm of aminofluorene or to used engine oil, did not accumulate detectable amounts of premutagens, mutagens, or mutagenic glucuronides/aglucones ei ... | 1989 | 2565191 |
| elevation of sex steroids and inhibition of udp-glucuronyltransferase are out of phase during gonadal maturation in the common carp. | 1. plasma sex steroid concentrations, onset of gonadal maturation, and hepatic microsomal udp-glucuronyltransferase (udpgt) activities were followed under natural temperature and photoperiod in outdoor tanks, and under controlled laboratory temperature and photoperiod regimens in common carp (cyprinus carpio). 2. decreased activity of udpgt was out of phase with elevations in plasma testosterone and 17 beta-estradiol during gonadal maturation. 3. injection of pituitary extract induced final gona ... | 1989 | 2565184 |
| decoupling of horizontal cells in carp and turtle retinae by intracellular injection of cyclic amp. | 1. horizontal cells are electrically coupled through gap junctions. this is a disadvantage in elucidating the membrane properties of the cells. in order to block gap junctions, adenosine 3',5'-cyclic monophosphate (cyclic amp) or its analogues, dibutyryl cyclic amp and 8-bromo cyclic amp, were ionophoretically injected into horizontal cells of the carp or turtle retina. 2. before injection of the chemicals the input resistance of the cell was so low as to be unmeasurable, because the applied cur ... | 2008 | 2559975 |
| distribution of angiotensin i converting enzyme in male reproductive systems of various vertebrates and properties of the genital enzymes. | the distribution of the angiotensin i converting enzyme (ec 3.4.15.1, ace) in male reproductive systems of various vertebrates including non-mammalian species and properties of the genital aces were studied. in such mammals as rat, dog and pig, it has been found that ace activity is only distributed in testis and epididymis (especially in the epididymal semen), but not in accessory sex glands such as prostate, seminal vesicle and coagulating gland. in the rat and dog, most of or all epididymal a ... | 1989 | 2558511 |
| the sympathetic neuro-melanophore transmission in a fresh-water indian major carp, labeo rohita (ham.). | adrenaline was effective in aggregating the melanosomes both in innervated as well as denervated melanophores. isotonic kcl could induce pigment aggregation only in innervated melanophores. adrenaline- and k+-induced pigment aggregation response in these melanophores was blocked by phentolamine: propranolol failed to do so. it is suggested that the chromatic nerves in the fish, labeo rohita are adrenergic and via post-synaptic alpha-adrenoceptors, control the melanosome aggregation. | 1989 | 2550366 |
| spin equilibrium in human methemoglobin: effects of inositol hexaphosphate and bezafibrate as measured by resonance raman spectroscopy. | the spin equilibria of several derivatives of human methemoglobin were probed by resonance raman scattering. the intensity of lines in the raman spectrum gives a measure of the high-spin (s = 5/2) to low-spin (s = 1/2) ratio which agrees well with the spin equilibria determined from direct magnetic susceptibility measurements. the addition of bezafibrate (bzf) to methemoglobin in the absence of organic phosphate, ihp, has very little effect on the spin equilibrium, whereas in the presence of ihp ... | 1989 | 2548610 |
| spin equilibria in human methemoglobin: effects of bezafibrate and inositol hexaphosphate as measured by susceptometry and visible spectroscopy. | the effects of inositol hexaphosphate (ihp) and a second allosteric effector, bezafibrate, on the spin-state equilibria of the mixed-spin derivatives of ferric human hemoglobin a are examined. changes in spin-state equilibrium are monitored by measuring absorption spectra in the visible region (460-700 nm) as well as by direct measurements of magnetic susceptibility by means of a superconducting fluxmeter. the addition of ihp at ph 6.5 results in a measurable shift in the spin equilibria of thes ... | 1989 | 2548609 |
| inward rectifier produced by xenopus oocytes injected with mrna extracted from carp olfactory epithelium. | ionic channels encoded by mrna extracted from carp olfactory epithelium were investigated by injection into xenopus laevis oocytes. the oocytes expressed an inward rectifier k+ -channel, as detected under two-electrode voltage clamp conditions. the results were as follows. an inactivating inward current appeared on hyperpolarization and increased with increasing extracellular k+ concentrations. the 0 current potentials plotted as a function of log [k+]0 in the range between 2 to 20 mmk+ fell on ... | 1989 | 2541514 |
| comparison of calpain i and calpain ii from carp muscle. | 1. the content of calpain ii is 3.4 times more than that of calpain i when estimated by the elution profiles from a column of deae-cellulose. 2. calpain i required 1 mm ca2+ and calpain ii required 5 mm ca2+ to show the full activities. these data demonstrated that ca2+-sensitivities of both calpains were lower than those of mammalian calpains, respectively. 3. the optimum caseinolytic activity was ph 7.2 for calpain i and ph 7.5 for calpain ii. 4. the molecular weight of calpain i was estimated ... | 1989 | 2539943 |
| the temperature dependence of the time course of growth and decay of miniature end-plate currents in carp extraocular muscle following thermal acclimation. | 1. the effect of temperature (5-35 degrees c) on the decay and growth phases of miniature end-plate currents (mepcs) was investigated in extraocular muscle from freshwater carp acclimated to either high (28 degrees c) or low (8 degrees c) temperature. 2. the temperature dependence of the time constant of decay (td) was found to follow an arrhenius relationship; the relationship between logtd and reciprocal of absolute temperature (1/k) being linear in both groups. the td of mepcs recorded from c ... | 1989 | 2533239 |
| the adductor mandibulae muscle in teleost fish with protrusible or non protrusible jaws: a histochemical and immunohistochemical study. | a study was made of the morphology and fibre type composition of the adductor mandibulae (am) muscle in teleosts with very protrusible (carp), moderately protrusible (cod) and non-protrusible (trout and cat-fish) jaws. in contrast to the trout and cat-fish, in which the am is formed by only 2 components (mandibular and mental), in the carp and cod there is a third portion (maxillary) which is more or less developed in relation to the extent of jaw protrusion. fibre types were identified by means ... | 1989 | 2527478 |
| structural differences between the ca2+-atpase enzymes of sarcoplasmic reticulum membrane from rabbit and carp muscles. | 1. structural features were compared in sarcoplasmic reticulum ca2+-atpase enzymes from carp (cyprinus carpio l.) and rabbit muscles. 2. both membrane preparations contained the 105,000 mol. wt ca2+ pump protein in high local density. 3. the tryptic cleavage of the carp enzyme gave different peptide fragments from those observed from rabbit enzyme. 4. addition of vanadate, ca2+ or lanthanides did not cause two-dimensional ca2+-atpase crystal formation, in contrast to the rabbit enzyme, which for ... | 1989 | 2522383 |
| acetylcholinesterase in the alimentary canal of fish: light and electron microscopic detection, quantitative distribution in different segments of the gut. | the acetylcholinesterase activity was measured and histochemically localized in the alimentary tract of 2 fish species, carp (cyprinus carpio) and tench (tinca tinca). a comparison was made of the activities in the different gut segments. light and electron microscopic histochemistry revealed acetylcholinesterase-positive cell bodies along the entire length of the alimentary canal in both species, between the muscular layers. acetylcholinesterase-positive, cholinergic motor endplates were freque ... | 1989 | 2516680 |
| an antioxidant dependent in vitro response of rainbow trout (salmo gairdneri) somatotrophs to carp growth hormone-releasing factor (grf). | 1989 | 2515141 | |
| optic nerve regeneration in adult fish and apolipoprotein a-i. | fish optic nerves, unlike mammalian optic nerves, are endowed with a high capacity to regenerate. injury to fish optic nerves causes pronounced changes in the composition of pulse-labeled substances derived from the surrounding non-neuronal cells. the most prominent of these injury-induced changes is in a 28-kilodalton (kda) polypeptide whose level increases after injury, as revealed by one-dimensional gel electrophoresis and autoradiography. the present study identified as apolipoprotein a-i (a ... | 1989 | 2494299 |
| evidence that a substance p-like peptide mediates the non-cholinergic excitatory response of the carp intestinal bulb (cyprinus carpio). | the participation of substance p in the noncholinergic contraction induced by transmural stimulation (tms) of the carp intestinal bulb was examined. in the presence of atropine, substance p caused the contraction of carp intestinal bulb smooth muscle in a concentration dependent manner (1 nmol/l - 1 mumol/l). the ec50 value was 28 +/- 7 nmol/l (n = 6). substance p-induced desensitization (1 mumol/l for 15 min), decreased the response to substance p and the atropine-resistant contraction induced ... | 1988 | 2467211 |
| anti-dextran antibodies of carp detected by the enzyme-linked immunosorbent assay (elisa). | carp produce anti-alpha (1-6) dextran antibodies after immunization with a vaccine of leuconostoc mesenteroides b512. these antibodies can be determined by passive haemagglutination, quantitative precipitation or by elisa. in our hands the elisa has proved to be 100 times more sensitive than the passive haemagglutination test. we could not found any correlation between the anti-dextran activity estimated with the passive haemagglutination on the one and the elisa on the other hand. | 1988 | 2465680 |
| presence of a substance p-like peptide in an acid extract of the intestinal bulb of the carp (cyprinus carpio). | 1. the effect of an acid extract of the carp intestinal bulb (eci) on guinea-pig ileum longitudinal smooth muscle (gplm) and carp intestinal bulb longitudinal smooth muscle (ciblm) was examined. 2. eci caused a concentration-dependent contraction of gplm and ciblm. this eci-induced response was reduced by atropine to 30-40% of the control, indicating that part of the contracting activity of eci is attributable to acetylcholine. the atropine-resistant contracting activity of eci was not mediated ... | 1988 | 2464388 |
| the immune response of carp against dextran. an analysis of the idiotype heterogeneity. | the expression of a "species-specific" idiotype with specificity for alpha(1-6) dextran in carp is described. all tested carp anti-dextran antisera react with a rabbit anti-id (carp anti-dex) serum. the rabbit anti-id serum can be partially inhibited by the specific ligand, dextran t70 or isomaltodecaose but not or only weak with other, unrelated carbohydrates. these data confirm our statement, that a limited germline v-gene repertoire may code for antibodies in fish. | 1988 | 2463185 |
| effect of pesticides (atrazine and lindane) on the replication of spring viremia of carp virus in vitro. | atrazine and lindane are widely employed as pesticides in agriculture, which by wash off, could contaminate the aquatic environment. in this study, they were tested in vitro on the replication of the virus of spring viremia of carp (svc) in epithelioma papulosum cyprini cell line. they were dissolved first in dmso and then in medium at their solubility limit in water (28 and 10 mg/l). the results of the kinetic titers after 72 h showed no statistically significant difference between control and ... | 1988 | 2461139 |
| antigenic determinants on human choriogonadotropin alpha-subunit. ii. immunochemical mapping by a monoclonal antipeptide antibody. | in order to study antigenic site(s) present in the carboxyl-terminal part of the alpha-subunit of human choriogonadotropin (hcg-alpha), we attempted to produce site-specific antibodies directed against a 34-residue synthetic peptide analogous to region 59-92 of hcg-alpha. from a fusion experiment performed with a mouse injected with hcg-alpha-(59-92)-peptide conjugated to tetanus toxoid as immunogen, we selected a monoclonal antipeptide antibody (designated fa36) which has high binding activity ... | 1988 | 2455715 |
| contractile response to substance p in isolated smooth muscle strips from the intestinal bulb of the carp (cyprinus carpio). | 1. the effect of substance p on the mechanical activity of carp intestinal bulb smooth muscle was investigated in vitro. 2. bath-applied substance p (1 nm-1 microm) caused concentration-dependent contraction of the smooth muscle. the ec50 value was 20 +/- 3 nm (n = 13). 3. pretreatment with tetrodotoxin (780 nm) or atropine (500 nm) partially decreased the contractile response to substance p, while methysergide (3 microm) did not decrease the response. 4. the contractile response to substance p ... | 1988 | 2455618 |
| light microscopic characterization of glycoconjugates in secretory cells of the carp (cyprinus carpio) gill epithelium. | secretory products of granular and mucous cells in the gill epithelium of the carp, cyprinus carpio, were distinguished by their cytochemical reactions with peroxidase-labelled lectins and with the galactose oxidase (go)-schiff reagents. secretory products of granular cells reacted with lectins from triticum vulgaris (wga), arachis hypogaea (pna), dolichos biflorus (dba), glycine max (sab), and lotus tetragonolobus (lta). they also reacted with go-schiff reagents. after sialic acid cleavage with ... | 1987 | 2449406 |
| calcium action potential and its use for measurement of reversal potentials of horizontal cell responses in carp retina. | 1. in the carp retina perfused with a solution containing high-ca2+, ba2+ and some k+-channel blockers, the horizontal cell produced a regenerative ca2+ action potential when the cell was depolarized by bath application of l-glutamate (glu) or l-aspartate (asp). the action potential was triggered also by a transretinal electrical stimulation which evoked an e.p.s.p. in the horizontal cell. in this solution, some cells produced the action potential spontaneously. 2. the action potential had an ov ... | 1987 | 2445962 |
| sepharose gels as antigenic matrices for specific binding of anti-galactosyl antibodies. | from 100 ml of pooled normal serum taken from different species (man, cow, rabbit, chicken, duck, carp) 0.5 to 4.0 mg of high and low molecular weight anti-galactosyl antibodies could be isolated by affinity-chromatography on non-ligand-attached sepharose 4b. the antibodies bound to the agarose matrix were eluted with 0.5 m d-galactose containing buffers. using species-specific anti-immunoglobulin sera in immunoelectrophoresis, the immunoglobulin character of the protein fractions prepared was a ... | 1987 | 2444220 |
| oxygen bonding in human hemoglobin and its isolated subunits: a xanes study. | the x-ray absorption near edge structure (xanes) spectra of the human adult and foetal hemoglobin, of the isolated alpha and beta chains, in the oxygenated forms, and of the oxymyoglobin and carp oxyhemoglobin have been measured at the wiggler beam line of the frascati synchrotron radiation facility. the bonding angle of oxygen molecule at the iron site in these hemoproteins in solution, has been measured using the multiple scattering theory for data analysis. | 1987 | 2443133 |
| dense osmiophilic material in the surface of the olfactory bulb in the teleost cyprinus carpio l. | a dense osmiophilic structure was found associated to the basal lamina of the outer limiting glial membrane of the carp olfactory bulb. in certain sections this material appears as "beads" composed of small strand-like structures, and in others it seems to form a palisade-like structure. the possible origin and function of this structure is discussed. | 1987 | 2442244 |
| effect of gamma-hexachlorocyclohexane (lindane) on carp (cyprinus carpio). ii. effects of chronic intoxication on blood, liver enzymes, and muscle plasmic membrane. | a 30-day ingestion of gamma-hexachlorocyclohexane (lindane) by carp (cyprinus carpio) induced hypoglycemia without activation of two hepatic gluconeogenesis enzymes (fructose diphosphatase, ec 4.1.2.13, and glucose-6-phosphatase, ec 3.1.3.9) and hyponatremia and variations in muscle plasmic membrane-bound enzymes (especially cholinesterases, ec 3.1.1.7). after 109 days carps exhibited a decrease in natremia but no significant hypoglycemia. there was an activation of gluconeogenesis enzymes. impo ... | 1987 | 2440661 |
| effect of gamma-hexachlorocyclohexane (lindane) on carp (cyprinus carpio). i. effect of chronic intoxication on humoral immunity in relation to tissue pollutant levels. | lindane, the gamma-isomer of hexachlorocyclohexane, one of the most widely used insecticides, was incorporated into carp food for 109 days. the effects of 10, 100, or 1000 ppm of lindane in food on hematocrits and on antibody production against yersinia ruckeri bacterin in sera and mucus were followed for 79 days. no effect of lindane on these parameters and on spleen weight was observed. lymphoid organs, spleen and kidney, were as highly contaminated at the beginning of the inoculation (day 30) ... | 1987 | 2440660 |
| mechanism of the interaction between myelin basic protein and the myelin membrane; the role of arginine methylation. | the addition of solutions of bovine myelin basic protein to suspensions of unilamellar vesicles prepared from whole myelin suspensions results in the rapid equilibrium association of the vesicles into dimers, followed by time-dependent aggregation reactions. other cationic proteins also induce the dimerization of the vesicles and equilibrium constants for dimer formation are obtained for bovine myelin basic protein, lysozyme, polyhistidine and myelin basic protein from carp, which differs from t ... | 1987 | 2440426 |
| immune response of carps (cyprinus carpio l.) against dextran. | in carps no antibodies could be evoked by immunization with soluble high molecular weight dextran using doses of 1-5000 mg dextran per kg body weight. on the other hand, an immune response against dextran could be elicited by immunization with a vaccine of leuconostoc mesenteroides b512 as well as a dextran-bsa-conjugate. these carp anti-dextran antibodies showed precipitating and agglutinating activities with specificity against alpha linked dextran determined by the almost complete inhibition ... | 1987 | 2439389 |
| modulatory effects of ambient temperature and antigen dose on the induction of specific tolerance to different protein antigens in carp (cyprinus carpio). | 1987 | 2438707 | |
| urea tolerance of myofibrillar proteins of two elasmobranchs: squalus acanthias and raja tengu. | some biochemical properties of actomyosin and myosin from elasmobranchs, squalus acanthias and raja tengu are compared with those of a freshwater (cyprinus carpio) and a marine teleost (seriola quinquiradiata). whereas ca2+-atpase of teleost actomyosins are more stable in the absence of urea, the reverse is true for elasmobranchs up to 1.0 m urea. in contrast to that of teleosts, the mg2+-atpase of s. acanthias actomyosin shows an activation in the presence of urea, where as that of r. tengu per ... | 1986 | 2434054 |
| preparation and some properties of carp heavy meromyosin. | trypsin fragments carp myosin heavy chain into components of lower molecular weight. these changes are concomitant with the loss of ca2+-atpase and a weakening in actin-myosin interaction. as heavy meromyosin (hmm) prepared from myofibrils is more homogeneous, the above changes are due to overfragmentation of heavy chain mass. amino-acid composition of carp and rabbit hmm's are similar, but differences exist in response to dtnb and incorporation. | 1986 | 2434053 |
| studies on immunological properties of enolase from carp muscles (cyprinus carpio) after chemical modification of some amino-acid residues. | after modification of histidine residues, enolase from carp muscles lost the ability to precipitate with antibodies against native enzyme. this ability remained after nitration of tyrosine residues, but the immunoelectrophoretic pattern--localisation and height of arcs--differed from that of native enolase. minor changes of this pattern were found in enolase with modified tryptophan residues. | 1986 | 2430546 |
| telencephalic afferent nuclei in the carp diencephalon, with special reference to fiber connections of the nucleus preglomerulosus pars lateralis. | fiber connections of the nucleus preglomerulosus pars lateralis (pgl), which primarily provides afferent inputs to the telencephalon, were examined in carp by means of horseradish peroxidase tracing methods. the major afferent sources of pgl are the bilateral nucleus tuberis anterior and a few projections are found deriving from the ipsilateral nucleus ventromedialis thalami, nucleus centralis posterior, dorsal periventricular hypothalamus, and torus semicircularis. axons arising in the pgl can ... | 1986 | 2429725 |
| effects of direct immersion in antigen on immunological memory in young carp, cyprinus carpio. | the study asks whether, in fish, antigens encountered early in life can prime the immune system to yield memory responses on subsequent challenge with the same antigen and, if so, whether positive immunity or immunological tolerance is induced. the direct immersion method of vaccination was used to prime 4 week old carp, cyprinus carpio, and was compared with priming by injection. three different forms of antigen were used: the thymus dependent antigen, human gamma globulin (hgg) in soluble and ... | 1986 | 2429432 |
| karyotypes of cyprinus carpio and leuciscus cephalus. | karyological investigations on the common carp (cyprinus carpio) and chub (leuciscus cephalus) are described. the diploid chromosome complement in the carp was found to be 98, with 50 meta- to submetacentric chromosomes, 48 subtelo- to telocentric chromosomes, and an arm number of 148. chromosomal studies on the chub revealed the diploid chromosome complement to be 50, with 34 meta- to submetacentric chromosomes, 16 subtelo- to telocentric chromosomes, and an arm number of 84. | 1986 | 2428561 |
| cloning and sequence analysis of cdnas encoding precursors of urotensin ii-alpha and -gamma. | the primary structures of the precursors of urotensin ii (uii)-alpha and -gamma, neuropeptide hormones of the caudal neurosecretory system of the carp, cyprinus carpio, have been determined by analyzing the nucleotide sequences of cloned dnas complementary to the mrnas encoding them. a cdna library, constructed with poly(a)+rna in the preterminal spinal cord, was screened using 32p-labeled synthetic oligonucleotides representing all possible cdna sequences corresponding to the pentapeptide commo ... | 1986 | 2427672 |
| the differentiation of germ cells and gonads during development of carp (cyprinus carpio l.). a study with anti-carp sperm monoclonal antibodies. | gonadal development, germ cell differentiation and the appearance of membrane antigenic determinants, specific for male and female germ cells during gonadogenesis, was studied in larval and juvenile carp (cyprinus carpio l.) until 25 weeks after fertilization. indirect immunofluorescence studies with four monoclonal antibodies raised against carp spermatozoa revealed that monoclonal antibody wcs 29 stained the outer membranes of primordial germ cells in larvae from 3 days after fertilization. th ... | 1985 | 2422310 |