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bipolar-amacrine synaptic transmission: effect of polarization of bipolar cells on amacrine cells in the carp retina. 19863022200
[accumulation of cyclic adenosine monophosphate in the ovary of the eel (anguilla anguilla l.) in vitro under the effect of carp gonadotropin or ovine lutropin: kinetics and thermodependence].cyclic amp (camp) in pieces of eel ovary was greatly increased in vitro by the gonadotropin (cgth) of carp, another teleost fish; after one hour at 20 degrees c, maximal stimulation = 31.7 and e.d. 50 = 0.08 micrograms/ml. ovine lutropin (olh) had less effect (maximal stimulation: 2.35; e.d. 50: 1.42 micrograms/ml); its action suggested that it involved a subfraction (olh/cgth rac) of the receptor-adenylate cyclase (rac) systems which mediate the action of cgth. another difference was the percen ...19863012723
[synaptic interactions of individual motor neurons of the spinal cord of the carp].the interaction between motoneurons was studied in the isolated spinal cord of the fish (cyprinus carpio) by the recording of elementary epsps evoked in a motoneuron by intracellular stimulation of adjacent motoneuron. these epsps are mediated mainly by electrical transmission as evidenced by their short or negligible latencies, stable amplitudes and bidirectional transmission between motoneurons. in some cells double-component elementary epsps were revealed which indicate dual (electro-chemical ...19863012381
dendritic morphology of dopaminergic cells revealed by intracellular injection of lucifer yellow in fixed carp retina.the dendritic morphology of dopaminergic cells in carp retinas was investigated by identifying their fluorescent cell bodies in isolated, aldehyde-fixed preparations and injecting them iontophoretically with lucifer yellow ch (ly) under microscopic control. the ly-injected cells were examined in flatmount and in radial cryosections. the cell bodies were located at the inner margin of the inner nuclear layer (the amacrine cell sublayer), and gave rise to 3-5 primary dendrites that branched repeat ...19863011192
complete nucleotide sequence of the matrix protein mrna of vesicular stomatitis virus (new jersey serotype).the complete nucleotide sequence of the mrna of the matrix (m) protein of vesicular stomatitis virus [new jersey serotype, vsv(nj)] was derived from a cdna clone and mrna. the mrna is 758 nucleotides long (excluding polyadenylic acid) and encodes a protein of 229 amino acids. the predicted amino acid sequence was compared with that of the corresponding protein of indiana serotype [vsv(ind)] and a fish rhabdovirus, spring viremia of carp virus (svcv). an amino acid identity of 62% was found betwe ...19863006343
a signal sequence is required for the functions of the signal recognition particle.it is shown that removal of the signal sequence of carp preproinsulin by gene technology abolishes the interaction between the polypeptide chain and the signal recognition particle with respect to both its functions: the inhibition of translation by the signal recognition particle in the absence of microsomal membranes, and the reconstitution of the protein translocation competence of high-salt inactivated microsomes. it is concluded that a signal sequence is absolutely required for the function ...19863004463
internally transposed signal sequence of carp preproinsulin retains its functions with the signal recognition particle.it is shown that the signal sequence of carp preproinsulin is functional with the dog pancreatic signal recognition particle (srp) both when present at its normal location at the amino-terminus of the protein or when engineered to an internal location. inhibition of translation by srp in the absence of microsomal membranes, reconstitution by srp of the translocation competence of high-salt inactivated microsomes and signal peptide cleavage all occur with the signal sequence being preceded by a h ...19863000822
viral diseases of fish: first report of carp pox in golden ide (leuciscus idus) in north america.carp pox, a putative viral disease exotic to north america, occurred in golden ide 1 yr after the fish were imported into the united states from the federal republic of germany. the raised, white, plaque-like lesions, which occurred on about 5% of the fish, healed spontaneously and caused no mortality. electron micrographs showed herpesvirus-like particles associated with lesion specimens; however, no infectious viruses were detected in tests with seven warmwater fish cell lines.19852993681
detection and some properties of calpain ii (high-ca2+-requiring form of calpain) in carp (cyprinus carpio) erythrocytes.in order to examine the existence of calpain i, a low (micromolar)-ca2+-requiring form of calpain, in fish tissues, carp erythrocytes were chosen as the experimental material, since only calpain i is known to exist in mammalian erythrocytes. by deae-cellulose chromatography, calpain and calpastatin (specific inhibitor for calpain) were separated from carp erythrocyte hemolysate. carp erythrocyte calpain is classified as calpain ii, a high (millimolar)-ca2+-requiring form of calpain, from the res ...19852992873
mutual inhibitory effect of calpastatins on calpains from carp muscle, carp erythrocytes and rat liver.the inhibitory effect of calpastatin (specific inhibitor for calpain) on calpain (ca2+-dependent cysteine proteinase, ec 3.4.22.17) was examined using carp muscle, carp erythrocytes and rat liver preparations. a mutual inhibitory effect between calpains and calpastatins from different tissues and species was observed. the conservation of the inhibitory effect of calpastatin on calpain among vertebrates suggests that the calpain-calpastatin system may play a biologically fundamental and common ro ...19852992872
purification and properties of carp (cyprinus carpio) muscle calpain ii (high-ca2+-requiring form of calpain).calpain (ca2+-dependent cysteine proteinase) was purified to apparent homogeneity from carp muscle by the method of deae-cellulose, hydroxylapatite and ultrogel aca 34 column chromatographies. the purified enzyme is classified as calpain ii (high-ca2+-requiring form of calpain) from the effects of ca2+ concentration, ph and the antibiotics on the activity. carp muscle calpain ii was inhibited by rat liver calpastatin, the specific inhibitor for calpain. it is probable that the calpain-calpastati ...19852992871
latency of horizontal cell response in the carp retina.the response latency of horizontal cells in the carp retina was investigated by means of the stimulus-response crosscorrelation using a randomly modulated light of various wavelengths. r/g type cells produced two response components with some difference in latency; short-latency hyperpolarising response to green light and long-latency depolarising one to red light. the latency difference could not be made smaller than 14 ms by increasing the intensity of test light. these results were reconciled ...19852992160
human chorionic gonadotrophin and immature fish ovary: characterization and mechanism of the in vitro stimulation of cyclic adenosine monophosphate accumulation.human chorionic gonadotrophin (hcg) increased cyclic adenosine monophosphate (camp) in pieces of eel ovary in vitro. although the ed50 (about 0.1 micrograms/ml) was the same as for carp gonadotrophin (cgth), the maximal stimulation (close to 3 times basal level) was about 10 times lower than that for the fish hormone. the mechanism of this atypical action of hcg was studied using different approaches. human cg did not inhibit the action of cgth. desensitization experiments (cgth or hcg being inj ...19852985465
separation and partial characterization by high-performance liquid chromatography and radioimmunoassay of different forms of melanocyte-stimulating hormone from fish (cyprinidae) neurointermediate lobes.from neurointermediate lobe (nil) extracts of two species of cyprinidae, carassius auratus and cyprinus carpio, several peptides were separated by high-performance liquid chromatography (hplc) on a c18 mubondapak column eluted with a methanol/acetic acid/triethylamine mixture. monitoring all fractions by radioimmunoassay (ria) with an antibody against melanocyte-stimulating hormone (msh) c terminal gave positive reactions for fractions 7, 11-12, 15-16, 23-24, and 25-27. for further characterizat ...19852984088
[synaptic mechanisms of the responses of olfactory bulb neurons of the carp to odorants].analysis of synaptic responses to some odorants in 16 secondary olfactory neurons and 5 granule cells of carp olfactory bulb revealed that the periodic discharges of secondary neurons, synchronous with the induced waves, were generated on the basis of dendrodendritic inhibitory interaction with granule interneurons. the mechanisms of tonic, asynchronous discharges in some secondary neurons remained not quite clear.19852983240
reexamination of the evidence for paramagnetism in oxy- and carbonmonoxyhemoglobins.experiments have been carried out in an attempt to confirm previous reports of paramagnetism in the oxy- and carbonmonoxy derivatives of human and carp hemoglobin. when care is taken to ensure complete saturation of the hemoglobins with ligand and the diamagnetic contributions of all of the buffers are carefully evaluated, these hemoglobin derivatives are found to have the same gram susceptibilities as exhibited by a set of metal free proteins, suggesting that they are fully diamagnetic.19852982138
[formation of muscle proteins during freezing].the effect of different conditions on the formation and properties of cryogels prepared by the freezing-thawing procedure from suspensions and solutions of the carp (cyprinus carpio) myofibrillar proteins was studied. the freezing of water solutions and suspensions of the native myofibrillar proteins resulted in the formation of the structures mainly stabilized by non-covalent bonds. when muscle proteins were denatured prior to the freezing they formed the structures stabilized by both non-coval ...20132977439
transferability of immune plasma and pronephric cells in isogeneic, allogeneic and xenogeneic transfer systems in crucian carp.transfer of immunity to horse erythrocytes (hrbc) by intravascular injection of immune plasma and pronephric cells was performed using histocompatible and histoincompatible crucian carp to examine "mhc" regulation in adoptive immunity. clonal ginbuna crucian carp, carassius gibelio langsdorfii and outbred kinbuna crucian carp, c. carassius buergeri immunized with hrbc were used as donors of plasma and cells for transfer to unimmunized members. recipients were then evaluated by measuring the anti ...19872960574
applicability of coagglutination test for detecting aeromonas salmonicida antigens from extracts of bacterial cultures and experimentally infected tissues of the common carp (cyprinus carpio l.). 19862953200
biology and histopathology of thelohanellus hovorkai achmerov, 1960 (myxosporea, myxozoa), a protozoan parasite of the common carp (cyprinus carpio). 19862953194
simple method for observing migrating leucocytes in the skin of mirror carp (cyprinus carpio l.). 19862953193
pink lateral muscle in the carp (cyprinus carpio l.): histochemical properties and myosin composition.the pink muscle of the carp differs from the white (and red) muscle not only histochemically but also in its myosin isoform, as shown by peptide maps of the myosin heavy chains. results of an electrophoretic analysis of myosins are discussed in the light of their immunohistochemical properties and histochemical atpase activity.19872952520
[new species of myxosporidians in the genus myxobolus (myxosporidia, myxobolidae) from cyprinid fishes of the amur river].five new species are described as follows: m. haematopterus from gills, fins and skin of cyprinus carpio haematopterus; m. gibelio from connective tissue of gill filaments, fin rays and kidneys of carassius auratus gibelio; myxobolus waleckii from gills of leuciscus waleckii; myxobolus junchisi from gills, muscles, kidneys and spleen of cyprinus carpio haematopterus; m. alacaudatus of cyprinus and carassius myxobolus divergens carassii from gills, muscles, fins, and cartilaginous tissue of operc ...20092947035
myofibrillar atpase activity in the carp cyprinus carpio: interactions between starvation and environmental temperature.the myofibrillar atpase activity of the epaxial white muscle was measured in carp cyprinus carpio l. acclimated to 10 degrees c or 28 degrees c. as previously reported, cold acclimation was associated with an increase in the atpase specific activity and a decrease in the thermostability. the water content of the white muscle was significantly higher in cold-acclimated fish than in warm-acclimated fish (p less than 0.002). starvation for 10 weeks resulted in a significant increase in the white mu ...19862943851
[specificity of carp antihuman myeloma protein sera depending on the holding temperature].while in carps kept in warm water (28 degrees c) and immunized with a human myeloma protein a challenge leads to an increase, the antibody titres in carps kept in cold water (14 degrees c) are changed only unsignificantly. antibody titres of carps immunized once respectively three times and kept in cold water are not markedly different. in testing the specificity of the antisera taken from each of the three groups no differences could be recognized. with carp anti-iggzei sera using the passive h ...19852936221
phospholipid content in the compact and spongious musculature of the carp heart (cyprinus carpio).the phospholipid content of the spongious and compact musculature of the heart of the carp (cyprinus carpio) was compared. the content of phospholipids is higher in the spongious musculature, the greatest difference being in the content of diphosphatidylglycerol: 2.53 mumol p . g-1 wet weight in the spongious layer and 1.29 mumol p . g-1 wet weight in the compact one. in both tissues plasmalogens represent 20-27% of the tissue content of choline and ethanolamine phosphoglycerides.20102932757
the influence of antibiotics on the immune system. ii. modulation of fish leukocyte responses in culture.we report the immunomodulating effect of two tetracycline analogues (oxytetracycline and doxycycline) on mitogenic and allogeneic stimulation of carp (cyprinus carpio) leukocytes in vitro. both drugs interfered with 3h-thymidine incorporation into the dna of phytohaemagglutinin-stimulated pronephric leukocytes in a dose-dependent manner. low concentrations of oxytetracycline delayed the mitogenic response, but did not reduce it. in addition, several other antibiotics were tested for their possib ...19852930938
new carp park ahead. 19892927496
age-dependent changes in volume and haemoglobin content of erythrocytes in the carp (cyprinus carpio l.).carp erythrocytes were fractionated by angle-head centrifugation which yielded fractions with a linear increase in density. haematological examinations revealed that the heavier red blood cells of carp had greater volumes (mcv), more haemoglobin (mch) and higher haemoglobin concentrations (mchc) than light ones. the same experiments with human red cell fractions yielded a decrease in mcv, constant mch and an increase in mchc. haemoglobin content in individual erythrocytes was also determined by ...19892926316
h1 horizontal cells of carp retina have different postsynaptic mechanisms to mediate short- versus long-wavelength visual signals.vertebrate photoreceptors release neurotransmitter substance(s) tonically in the dark and this release is curtailed by light. recently, we have become increasingly aware of the possibility that short- and long-wavelength visual signals are mediated differently during the synaptic transmission to second-order retinal neurons. the experiment described here advances this notion further by demonstrating a postsynaptic difference. treatment of the carp retina by dopamine reduced the gap-junctional co ...20102924847
enzymatic properties of fish muscle aldolase.1. aldolases were isolated from the ordinary muscle of red sea bream pagrus major, pacific mackerel scomber japonicus, and carp cyprinus carpio by ammonium sulfate fractionation, followed by ion-exchange chromatography on deae-cellulose and cm-sepharose cl-6b columns, and examined for enzymatic properties. 2. the aldolases showed the highest activity in a ph range from 6.8-7.8 km values for fructose-1,6-bisphosphate ranged from 0.025-0.10 mm. 3. irrespective of fish species, aldolase activity wa ...19892924547
purification and properties of pituitary gonadotropic hormone from indian teleosts: freshwater murrel (channa punctatus) and carp (catla catla).gonadotropic hormone (gth) from the pituitaries of two widely different indian teleosts, a freshwater murrel and a carp, was purified by solvent fractionation, sephadex g-100 gel filtration, affinity chromatography on concanavalin a-sepharose (con a-sepharose), and immunoaffinity chromatography. elution profile from gel filtration showed three peaks in both cases, peak i and ii were clearly separated in murrel but not in carp. peak ii demonstrated strong gth activity in both murrel and carp but ...20072920895
purification of carp growth hormone and cloning of the complementary dna.the growth hormone (gh) was isolated and purified from common carp (cyprinus carpio) pituitary glands by salt precipitation and hplc on reverse-phase c18 columns. the carp gh cdna was synthesized and cloned in escherichia coli using ecori linkers and pbr322 as vector. the positive clones were selected and sequenced. the full-length carp gh cdna contains 1187 nucleotide basepairs with an open reading frame coding for the precursor form carp gh of 210 amino-acid residues. the partial amino-acid se ...19892920175
xanes spectroscopy sensitivity to small electronic changes. case of carp azidomethemoglobin.spin states equilibrium of hemoglobin-iron varies with external conditions: ph, allosteric effectors, temperature. the small electronic reorganization of the iron caused by the spin state changes has been detected by x-ray absorption near edge structure (xanes) spectroscopy at room temperature. the iron k-edge region which is sensitive to spin state is located in 7110-7130 ev. here are presented the 100% high spin and 100% low spin xanes spectra of carp azido ferric hemoglobin.19892914617
electrocardiograms in five bipolar leads recorded from the body surface of three fish species (cyprinus carpio, oreochromis niloticus and pagrus major) in fresh or sea-water.1. ecgs were recorded using five bipolar leads from the body surface of porgy pagrus major and tilapia oreochromis niloticus in fresh or sea-water, or held on a dry towel. 2. the maximum qrs amplitude was detected in lead v in both species being 54 +/- 25 microv in porgy and 241 +/- 78 microv in tilapia, respectively, when the fish were held on the dry towel. 3. clear ecg waves could not be obtained from porgy in sea-water because of the porgy's small cardiac potential and leakage of the potenti ...19882907442
effects of a high concentration of co2 on electrocardiograms in the carp, cyprinus carpio.1. the effects of a high concentration of co2 (pco2 = 250 mmhg and po2 = 360 mmhg in water) and ms222 (tricaine methanesulfonate, 1/8000 or 1/5000) on the electrocardiogram (ecg) in carp were examined using five kinds of bipolar leads from the body surface. 2. in the carp anesthetized with the high concentration of co2 for 30 min, the qrs duration, pq interval and and direction of the qrs axis on the frontal plane significantly changed. even after recovery from anesthesia, delay in the qrs durat ...19882907441
effects of metal ions on the antioxidant enzyme activities, protein contents and lipid peroxidation of carp tissues.1. studies were performed regarding the effects of cuso4 in concentrations of 5, 10, 25 and 50 ppm and znso4 in concentrations of 10 and 100 ppm on the antioxidant enzyme activities, lipid peroxidation and protein contents of tissues of common carp (cyprinus carpio morpha l.) exposed to these pollutants for 24 hr. 2. the results demonstrated that cuso4 was more toxic than znso4 and that both treatments brought about significant changes in these parameters in carp hepatopancreas (liver), gill and ...19882904875
the effect of isoprenaline on the phospholipid content of the compact and spongious musculature of the carp ventricular myocardium.1. after a single injection of 40 mg kg-1 of isoprenaline to the carp, lysophospholipids appear in the tissue of the heart ventricle, ethanolamine plasmalogens increase and choline plasmalogens decrease; phosphatidylinositol is lowered in the spongious layer only. 2. daily administration of 5 mg kg-1 of the drug leads, after 5 doses, to a dramatic decrease of the diphosphatidylglycerol content; during the subsequent 5 and 10 doses a return to normal values occurs. shifts in plasmalogens are simi ...19882904868
scrapie-associated fibrils, prp protein and the sinc gene.scrapie-associated fibrils (saf) are disease-specific structures found in extracts of the brains of animals affected with scrapie. these structures are pathological aggregates of a normal host protein called prp. in collaboration with konrad beyreuther (heidelberg), we have characterized the multiple forms of prp found in saf fractions from mouse brain affected by the me7 strain of scrapie. there is no in vivo n-terminal cleavage of the most abundant forms of prp. however, n-terminal cleavage of ...19882900718
influence of heat treatment on osmotic fragility of carp erythrocytes. 19882900285
pharmacological properties of the atropine-resistant contraction of the carp (cyprinus carpio) intestinal bulb induced by transmural stimulation.1. the pharmacological properties of the atropine-resistant contraction of the carp intestinal bulb induced by transmural stimulation were investigated. 2. in the presence of atropine (1 microm), transmural stimulation caused frequency-dependent (2-50 hz) contraction which was abolished by tetrodotoxin (780 nm). 3. the atropine-resistant contraction was not decreased by tubocurarine (5 microm), hexamethonium (100 microm), carteolol (5 microm) and phentolamine (5.4 microm). 4. in vitro pretreatme ...19872893677
reversal potentials of rod horizontal cell responses in the carp retina. 19872891094
a d-mannose-specific lectin from gerardia savaglia that inhibits nucleocytoplasmic transport of mrna.a new lectin has been isolated from the coral gerardia savaglia by affinity chromatography, using locust gum as an absorbent, and d-mannose as eluant. final purification was achieved by bio-gel p300 gel filtration. the agglutinin is a protein composed of two polypeptide chains with a mr of 14800; the two subunits are not linked by disulfide bond(s). the isoelectric point is 4.8, the amino acid composition is rich in the acidic amino acids aspartic acid and glutamic acid. the absorption maximum f ...19872890521
metabolic activation of 2-aminofluorene, 2-acetylaminofluorene and n-hydroxy-acetylaminofluorene to bacterial mutagens with mussel (mytilus galloprovincialis) and carp (cyprinus carpio) subcellular preparations.1. the mode of activation of 2-aminofluorene (af), 2-acetylaminofluorene (aaf) and n-hydroxy-acetylaminofluorene (oh-aaf) to salmonella typhimurium ta 98 mutagens was investigated in subcellular fractions from the digestive gland of the mussel mytilus galloprovincialis and from the liver of carp cyprinus carpio. 2. in carp liver microsomes the activation of oh-aaf was due to very active deacetylase, in contrast to undetectable deacetylase-dependent activation in mussel microsomes. 3. af and aaf ...19872890481
the interplexiform-horizontal cell system of the fish retina: effects of dopamine, light stimulation and time in the dark.interplexiform cells contact cone horizontal cells in the fish retina and probably release dopamine at synaptic sites. the effects of dopamine, certain related compounds, and light and dark régimes were tested on the intracellularly recorded activity of horizontal cells in the superfused carp retina to elucidate the functional role of the interplexiform cell. dopamine application onto retinae kept in the dark for 30-40 min increased the size of the responses of cone horizontal cells to small-spo ...19872888119
dendritic morphology of indoleamine cells revealed by intracellular injection of lucifer yellow in fixed carp retina.the dendritic morphology of indoleamine amacrine cells in carp retina was investigated by identifying their fluorescent cell bodies by preloading with noradrenaline followed by iontophoretic injection of lucifer yellow in isolated and aldehyde-fixed preparations under microscopic control. although two subpopulations of serotonin-like immunoreactive amacrine cells (small and large in soma size) were found, small cells were not seen in aldehyde-fixed preparations. cells preloaded with noradrenalin ...19872888047
[subcellular localization of glutamine synthetase activity in carp muscle tissue and liver].subcellular distribution of the glutaminesynthetase activity (ec 6.3.1.2) is studied in muscle tissue and liver of carp. it is established that this activity is distributed by subcellular fractions analogously to the glutamatedehydrogenase activity--enzyme-marker activity of mitochondria. an additional evidence for the association of the two given enzymes is obtained during equilibrium density-gradient centrifugation of mitochondria on 30-60% gradient of sorbitol. comparison of the obtained resu ...20152885952
paraquat as an agent affecting antioxidant enzymes of common carp erythrocytes.the effects of paraquat on the superoxide dismutase, catalase, glutathionine peroxidase activities and lipid peroxidation at different times of paraquat exposure of cyprinus carpio morph l. erythrocytes were studied. typical characteristics were observed in the changes of the enzyme activities of the erythrocytes after exposure to paraquat. the haemoglobin concentration of common carp haemolysates was decreased by exposure to paraquat.19872885134
the effect of various ambient ammonia concentrations on the nitrogen metabolism of carp fry (cyprinus carpio l.).authors studied how 125, 375 and 625 micrograms/l amounts of nh4cl added to the water influenced the ammonia excretion of carp fry with an average weight of 2.4 g, compared to the control. during the course of the experiments the nh4n concentration, the ph and the temperature were measured for three days, twice daily. on the basis of our results the threshold concentration exerting harmful effects on the ammonia household of carp fry (disturbing the normal rate of metabolism in the fishes and de ...19872881685
carp maturational-ovulatory gonadotropin but not carp vitellogenic gonadotropin or salmon maturational-ovulatory gonadotropin stimulates testosterone production by rat leydig cells in vitro.carp (cyprinus carpio) maturational-ovulatory gonadotropin, prepared from the fraction of pituitary extract adsorbed on con a-sepharose (con a ii) and subsequently adsorbed on cm-cellulose (whatman cm-52), stimulated testosterone production by isolated rat leydig cells. the fraction of carp pituitary extract unadsorbed on the immobilized lectin (con a i) with a mol. wt of 30,000, which had previously been shown to contain vitellogenic gonadotropin, was devoid of steroidogenic activity. salmon (o ...19872881654
hormones with adrenocorticotropic and opiate-like activities from the carp (cyprinus carpio) pituitary.carp (cyprinus carpio) pituitary acetone powder was extracted with a mixture of water, hydrochloric acid and acetone. an acid acetone powder was formed by adding the pituitary extract into a large volume of chilled acetone and subsequently recovering the precipitate. the powder was subjected to ion exchange chromatography on cm cellulose. fractions adsorbed on the ion exchanger exhibited acth-like activity as evidenced in the ability to stimulate lipolysis in isolated hamster adipocytes and cort ...19862879705
phospholipid dependency of carp brain and liver mitochondrial monoamine oxidase.the effects of lipid-protein interactions on carp brain and liver mitochondrial mao with respect to substrate and inhibitor preference, thermostability and arrhenius parameters were studied and compared. treatment with phospholipase a2, c or d decreased mao activities towards 5-hydroxytryptamine (5-ht), beta-phenylethylamine and tyramine similarly, accompanied by great changes in their apparent affinities for mao, but not by changes in vmax values. minimum phospholipid binding to mitochondria mi ...19862879700
clastogenic effects of five carcinogenic-mutagenic chemicals on the cells of the common carp, cyprinus carpio l.the cytogenetic effects of various i.p. treatments with five carcinogenic-mutagenic chemicals and three doses for each (aflatoxin b1, aroclor 1254, benzidine, benzo[a]pyrene and 20-methylcholanthrene), were investigated in the cells of the common carp, cyprinus carpio. injection with distilled water and corn oil served as the two control groups. for detecting cytogenetic damage we used two test systems, chromosomal aberrations (ca) in kidney cells and micronucleated erythrocytes (m). at 48 hr af ...19862877805
electrocardiograms recorded from the body surface of the carp, cyprinus carpio.the electrocardiograms (ecgs) in five kinds of bipolar leads were recorded from the body surface of the carp under water. each component constituting the ecg wave could be detected by the present method. the potentials of the ecgs recorded by the present method were relatively small, the values being 2-64 microv. the electrical axis in most carp was directed toward the right lateral side.19862876807
the time-course of appearance and net accumulation of horseradish peroxidase (hrp) presented orally to juvenile carp cyprinus carpio (l.).a sensitive enzyme-linked immunosorbent assay (elisa) technique was used in order to determine horseradish peroxidase (hrp) uptake from the carp gut. hrp was detected in blood plasma and various tissues within 15 min of oral intubation. the time-course of net accumulation (uptake-degradation) over a 2 hr period was recorded. the presence of hrp reached a maximum in the body tissues approximately 1 hr after intubation and on a microgram/g wet weight basis the order of accumulation within the tiss ...19862875835
effects of morphine and methionine-enkephalin on the smooth muscle tonus and the contraction induced by transmural stimulation in the carp (cyprinus carpio) intestinal bulb.the effects of morphine and methionine-enkephalin (met-enkephalin) on the smooth muscle tonus and the contraction induced by transmural stimulation were investigated in the isolated intestinal bulb of carp in vitro. morphine (30 nm-3 microm) and met-enkephalin (3 nm-5 microm) caused dose-dependent non-sustained contraction. naloxone (10 nm) inhibited the contraction induced by morphine or met-enkephalin in a competitive manner. tetrodotoxin (400 nm) or atropine (500 nm) did not inhibit the contr ...20092874947
factors affecting release of 3h-dopamine from perfused carp retina.the effects of putative retinal neurotransmitters and several neuropeptides on 3h-dopamine release from isolated perfused carp (cyprinus carpio) retinas were studied. of the transmitter candidates tested, only serotonin (5-ht) and the 5-ht agonist tryptamine released 3h-dopamine. however, the release evoked by these agents was calcium (ca2+) independent and not blockable by the 5-ht antagonist methysergide. we also investigated the antagonism of inhibitory inputs as a potential regulatory mechan ...19862874198
the in vitro metabolism of eulan wa new by liver homogenates from freshwater fish.the rate of formation of pentachloro-2-aminodiphenylether (5-pad) from pentachloro-2-(chloromethylsulphonamido) diphenylether (6-pcsd) by goldfish liver homogenates was studied in a variety of buffers. the highest rate was observed when the homogenate was prepared and assayed in phosphate buffer containing dithiothreitol. the 5-pad forming activity of liver homogenate preparations from a variety of freshwater fish (goldfish, carp, pike, rainbow trout, perch and eel) showed contrasting species di ...19862873942
presynaptic alpha-adrenoceptor mediated inhibition of the neurogenic cholinergic contraction in the isolated intestinal bulb of the carp (cyprinus carpio).the effects of norepinephrine, epinephrine and clonidine on neurogenic cholinergic contraction were examined in the presence of a beta-adrenoceptor blocking agent, carteolol (5 x 10(-6) m), in the isolated intestinal bulb of the carp. norepinephrine, epinephrine (10(-9)-10(-6) m) and clonidine (10(-8)-10(-5) m) inhibited the contraction induced by low frequency (2 or 5 hz) transmural stimulation (tms) without inhibiting the contraction induced by acetylcholine (ach, 6 x 10(-8)-4 x 10(-7) m). met ...19862871988
beta-2 adrenergic receptors are not only for circulating catecholamines in ventricular muscles of carp heart (cyprinus carpio).whether beta-2 adrenergic receptors are only for the circulating catecholamines in isolated ventricular muscles of carp heart was studied. isoproterenol, epinephrine and norepinephrine had concentration dependent positive inotropic effects. the ed50 values for isoproterenol, epinephrine and norepinephrine were 6.23 +/- 1.9, 87.3 +/- 27.3 and 4500 +/- 580 nm, respectively. phentolamine did not alter the inotropic effects. tyramine increases the force of contraction and this action was completely ...19862871987
are beta-adrenergic receptors in ventricular muscles of carp heart (cyprinus carpio) mostly the beta-2 type?the positive inotropic effect of isoproterenol was quantified in the presence of several beta-adrenergic blocking agents in ventricular strips of carp heart. isoproterenol had a concentration-dependent positive inotropic effect. the effect was markedly inhibited by propranolol and carteolol, but was extremely insensitive to atenolol. practolol totally failed to alter the effect. these results indicated that the positive inotropic effect of isoproterenol may be mediated by mostly beta-2 adrenergi ...19862871986
n-methyl d-aspartate acts as an antagonist of the photoreceptor transmitter in the carp retina.glutamate, aspartate, and their agonists, kainate, quisqualate, cysteine sulfinate and n-methyl-d-aspartate (nmda), were applied to the isolated carp retina while recording from horizontal cells. all these agents, except nmda, depolarized horizontal cells membrane and reduced responses to light, thus mimicking the effect of the endogenous photoreceptor transmitter. application of nmda, on the other hand, caused a membrane hyperpolarization of horizontal cells in the dark, an effect different fro ...19862871897
histaminergic mechanisms in the vertebrate visual system.this paper presents and summarizes our recent results on histamine (hi) in vertebrate visual system. the data include: 1. hi distribution, localization, synthesis, uptake and metabolism in retina, optic nerve, choroid and brain of carp, hen, rabbit, cow, 2. distribution of hi in human eyes, 3. 3h-mepyramine binding in the bovine retina, and hi dependent camp generating system in the rabbit eye, 4. effect of light stimulation on hi content in the rabbit retina, optic nerve and brain, 5. diurnal v ...19852870485
sublethal effect of ammonia on certain biochemical and haematological indicators in common carp (cyprinus carpio l.).carp (cyprinus carpio l.) fry were exposed for 3 weeks to a sublethal concentration of ammonia (0.102 +/- 0.059 mg nh3/dm3). ammonia induced considerable changes in the level of free amino acids and unfavourable changes in the blood, i.e. leucopaenia and eosinophy. the observed increase of the concentration of most amino acids in fish intoxicated with nh3 suggests that the process reflects detoxication of ammonia which takes place both in the brain and muscles.19862869895
anaerobic wintering of crucian carp (carassius carassius l.)--ii. metabolic products.in long-term experimental anoxia (up to 140 days) crucian carp excreted ethanol and acetic acid. the maximum concentration of ethanol found in fish blood was ca 0.1%. the excretion rate of ethanol at temperatures from -0.5 to +5 degrees c was ca 25 micrograms/g fish wet wt/hr, but increased rapidly with increasing temperature. at 2 and 5 degrees c the ethanol excretion rate was independent of fish size, but at 12 and 18 degrees c a higher rate was observed in smaller fish. the decrease of water ...19862869864
isoproterenol-induced desensitization to the positive inotropic effect of isoproterenol in ventricular strips isolated from carp heart (cyprinus carpio).whether the positive inotropic effect of isoproterenol in ventricular strips of carp heart is altered by previous exposure to the agonist was studied. isoproterenol produced a concentration-dependent positive inotropic effect in these preparations which was competitively antagonized by propranolol. isoproterenol dose-response curves were shifted significantly downward and to the right after previous treatment with and removal of isoproterenol. the desensitization could be demonstrated after 5 mi ...19852866913
the effects of pentachlorophenol, phenol and other pollutants on the liver of carp (cyprinus carpio l.).liver tissue of carp was kept in roller tubes and the basal and epinephrine-induced release of glucose after 6 or 24 hr incubation were measured. the amount of liver glycogen after incubation was also determined. the liver was taken from carp treated in vivo with pollutants, mainly pcp or phenol, or was exposed to these pollutants in vitro. treatment of carp in vivo with 10-10(4) micrograms/l phenol reduced the basal and the epinephrine-induced release of glucose from the liver. treatment with l ...19852866901
some observations on the influence of stress on ecg patterns in oreochromis mossambicus and cyprinus carpio.electrocardiograms of undisturbed, resting fish as well as disturbed (stressed) fish were recorded by biotelemetry. both fish species showed marked changes in heartbeat patterns in response to stress with tilapia exhibiting the most pronounced changes. a theory is advanced to account for the marked changes in electrocardiogram patterns of disturbed fishes.19852866875
effects of acidic amino acid antagonists upon the spectral properties of carp horizontal cells: circuitry of the outer retina.the acidic amino acid receptor antagonists, alpha-methylglutamate and alpha-aminoadipate, were applied to the carp retina to study their effects upon the spectral properties of horizontal cells and to elucidate the synaptic connections between horizontal cells and cones. application of these antagonists strongly hyperpolarized the l-type cone horizontal cells and reduced the responses of these horizontal cells to red light more than to blue light. application of co2+ ions to the retina, a proced ...19852865339
effect of pesticides on the ldh activity and isoenzyme pattern of carp (cyprinus carpio l.) sera.copper sulphate (cuso4), paraquat (pq) and methidathion (md) all caused tissue necrosis as demonstrated by increased levels of lactate dehydrogenase (ldh, ec 1.1.1.27) in blood sera. the main target of damage is shown by the isoenzymes appearing in the blood sera. the cuso4 treatment damaged mainly the heart and skeletal muscles. this is indicated by the increased ldh level and the m and h subunits appearing in the isoenzyme pattern of the serum. pq damaged first of all the gills and methidathio ...19852865066
so-called interplexiform cells immunoreactive to tyrosine hydroxylase or somatostatin in rat retina.the morphology of so-called interplexiform (ip) cells immunoreactive to tyrosine hydroxylase (th) or somatostatin (som) in the rat retina was described in comparison with those in the carp retina. in frozen cross-sections of the rat retina, many processes of th-like immunoreactive cells were found to extend toward the outer plexiform layer (opl), forming a thin layer of network fibers. a few of them further extended into the photoreceptor cell layer; such fibers were never found in the carp reti ...19852864980
a thrombelastographic study of the effect of stress on the blood coagulation in cyprinus carpio (cyprinidae) and oreochromis mossambicus (cichlidae).the normal blood coagulation in cyprinus carpio and oreochromis mossambicus was investigated by thrombelastography and standard thrombelastographs were obtained for each species. the effect of stress on blood coagulation was thereafter determined. the thrombelastograms indicated hypercoagulability under stress conditions. a prominent observation is the differences in the elasticity of the clot under various conditions. fibrinolysis occurred in all cases, except in the case of cyprinus carpio aft ...19852864206
the effect of paraquat on the peroxide metabolism enzymes in erythrocytes of freshwater fish species.the toxic effects of 10 ppm paraquat in vivo on the enzymes superoxide dismutase (sod), catalase (c), peroxidase (p), glutathione peroxidase (gsh-px) and on lipid peroxidation (lp) were estimated in erythrocytes of the carp, the tench and the crucian carp. paraquat caused activity enhancement of the peroxide metabolism enzymes and increase of the lipid peroxidation in the carp and the crucian carp. the enzyme activities and lipid peroxidation were dependent on the species and on the length of th ...19852861964
accumulation of pollutants in fish.the amount of radioactivity which derived from 14c-labeled pollutants was determined in liver, kidney, intestine, blood, muscle and gills of carp, exposed for 6, 24 and 72 hr to high external concentrations of urea, methanol, atrazine and pcp. the results allowed one to calculate roughly the uptake rate for these compounds. it was low for urea (0.055 micrograms/g per hr), higher for methanol (0.12) and atrazine (0.16) and highest for pcp (1.5). the bioaccumulation factors (bfs) were determined f ...19852861946
induction of metal-binding proteins by zinc is accompanied by reduction of other proteins including antibodies.exposure of carp lymphoid cells to zinc causes formation of metal-binding proteins (metallothioneins). metallothionein induction is accompanied by a suppression of the production of other proteins. antibodies are among the proteins which are decreased by zinc exposure. similar results from in vivo experiments indicate that zinc might affect even fish living in water still regarded as unpolluted.19852861052
membrane lipid environment of carp brain and liver mitochondrial monoamine oxidase.delipidation of carp liver mitochondria by treatment with methyl ethyl ketone (mek) or triton x-100 and then perchlorate greatly reduced mao activities. treatment with only triton x-100 resulted in less reduction in activity. the km values of the remaining activities were similar regardless of these treatments. the sensitivities towards clorgyline and l-deprenyl of the remaining activity in the triton x-100-treated residue and the phospholipase c-treated carp brain mitochondria were found to be ...19852861003
sodium-dependent absorption of inorganic phosphate by the carp intestine.concentrative absorption of inorganic phosphate observed in the stripped and everted intestine of the carp disappeared, when na concentration of the ringer solution was reduced from 150 to 7 mm, or when orthophosphate was replaced with metaphosphate. incubation in the ringer solution gassed with n2 instead of o2 also resulted in loss of the pi absorption. dinitrophenol significantly decreased the unidirectional pi flux from mucosa to serosa (jms) and increased the flux from serosa to mucosa (jsm ...19852858323
uptake and distribution of radioiodine, and the effect of ambient nitrate, in some fish species.125i uptake by the thyroid was most pronounced in the smelt (osmerus eperlanus), perch (perca fluviatilis), rainbow trout (salmo gairdneri), roach (rutilus rutilus) and bleak (alburnus alburnus). unlike other tissues, only the muscle in the species studied no relative accumulation of 125i as compared to the ambient water. the crusian carp (cyprinus carassius) indicated the lowest levels of accumulation of 125i among the species tested. thyroid radioiodine concentration was independent of the len ...19852858311
effects of acute heat exposure on respiratory metabolism and blood glucose in freshwater fishes, prochilodus scrofa (curimbatá) and cyprinus carpio (carp) acclimatized to tropical winter.routine oxygen consumption and blood glucose were determined from freshwater fishes, prochilodus scrofa and cyprinus carpio, exposed at high temperatures for 1 hr. prochilodus scrofa had a significantly higher rate of oxygen consumption at 30 degrees c than at 25 degrees c, and carp higher at 25 degrees c than at 30 degrees c. blood glucose was significantly higher for cyprinus carpio than for prochilodus scrofa at 25 and 30 degrees c; however, after exposure to these temperatures for 1 hr blood ...20102858306
in vitro absorption of inorganic phosphate and other electrolytes in the carp intestine.net movements of water, na, cl, k, ca, mg and pi were measured in the stripped and everted intestine of the carp. a high concentrative absorbtion of pi was observed, whereas na and cl were absorbed almost isotonically. the rate of pi absorption was greater in the middle part than in the anterior or posterior part of the intestine.19852858287
l-glutamate depolarizes on-off transient type of amacrine cells in the carp retina: an ionophoretic study.amacrine cells generating the on-off transient type of photoresponse, which is most frequently encountered, were identified by intracellular recording and staining with a fluorescent dye lucifer yellow in the carp (cyprinus carpio) retina. l-glutamate applied ionophoretically slowly depolarized the amacrine cells by about 10 mv and reduced their photoresponses during the application. such changes gradually recovered following the cessation of l-glutamate injection. the results indicate that this ...19852858255
[effect of baclofen and muscimol on the evoked potentials of the tectum mesencephali in the carp in response to stimulation of the visual and somatosensory afferent inputs].the perfusion of (+/-) 10(-5) m baclofen to the optic tectum of the carp greatly reduced evoked potential to the optic nerve stimulation and slightly inhibited evoked potential to the spinal cord stimulation. the perfusion of 10(-4) m muscimol reduced the former evoked potential as well as the latter one. the results permit supposing that tectal visual input is more sensitive to the presynaptic inhibitory influences through gabab-receptors than the somatosensory afferent input.19882854884
amino acid sequence and three-dimensional structure of cytosolic adenylate kinase from carp muscle.the amino acid sequence of cytosolic adenylate kinase from carp muscle has been determined. for the analysis of the blocked amino terminus an n-acylaminoacyl peptidase has been isolated and applied successfully. the resulting sequence is homologous to those of other adenylate kinases. it has 76% and 74% identical amino acids when compared with the enzymes from chicken and pig, respectively. the carp enzyme forms x-ray-grade crystals, the structure of which has been solved at 0.58-nm resolution. ...19882851785
[characterization of opioid receptor from bullfrog brain].among the tested five species--carp, bullfrog chicken, rat, and mouse--the solubilized fraction derived from the bullfrog brain gave the highest binding activity similar to that of membranes. the difference in their binding activities was demonstrated to depend on the bmax value, but not on kd. high-affinity binding of [3h]naloxone and [3h]ethylketocyclazocine (ekc) was proved in supernatant of detergent-treated membranes. the displacement curves of various [3h]ligands binding to the membrane an ...19882851236
analysis of synaptic inputs to on-off amacrine cells of the carp retina.to elucidate the synaptic transmission between bipolar cells and amacrine cells, the effect of polarization of a bipolar cell on an amacrine cell was examined by simultaneous intracellular recordings from both cells in the isolated carp retina. when either an on or off bipolar cell was depolarized by an extrinsic current step, an on-off amacrine cell was transiently depolarized at the onset of the current but no sustained polarization during the current was detected. the current hyperpolarizing ...19882849629
catch-relaxing peptide (carp) decreases the ca-permeability of snail neuronal membrane.effect of carp was investigated on snail neurones. it was found that carp decreased the slowly inactivating ca- and the ca-activated k-currents.19882848713
subtype of excitatory amino acid receptor in cone horizontal cells of the carp retina as specified by reversal potential measurement technique.effects of agonists of the excitatory amino acid (eaa) transmitters were examined in carp cone horizontal cells where glutamate (glu) or aspartate (asp) is believed to act as the transmitter released from the photoreceptors. bath application of kainic (ka), quisqualic (qa) and n-methyl-d-aspartic (nmda) acids produced little effect on cone cells, indicating that their effects act directly on the horizontal cells. ka and qa (100 microm for both) produced depolarizations in the horizontal cells. t ...19882840614
separation of gonadotropic fractions with different species specificities from tuna pituitaries.eight different gonadotropic glycoprotein fractions were separated from the acetone-dried powder of yellow fin tuna pituitary glands by successive chromatographies on superose 12 for gel filtration and mono q for anion exchange using the pharmacia fast protein liquid chromatography system. this was preceded by preliminary separations using an ammonium sulfate precipitation method and affinity chromatography on concanavalin a-sepharose. for biological characterization, we employed two radiorecept ...19882838377
cloning and characterization of a highly repetitive fish nucleotide sequence.we have cloned and sequenced a highly repetitive hindiii fragment of dna from the common carp cyprinus carpio. it represents a tandemly repeated sequence with a monomeric unit of 245 bp and comprises 8% of the fish genome. higher units of this monomer appear as a ladder in southern blots. the monomeric unit has been sequenced; it is a + t-rich with some direct and some inverse-repeat nucleotide clusters.20092835294
carp (cyprinus carpio) heart has a high sensitivity to the positive inotropic effect of strophanthidin despite negative force-frequency relationships.1. the relationship between response of the heart to increased stimulation frequency and digitalis sensitivity was examined comparing the positive inotropic effect of strophanthidin and [3h]ouabain binding to sarcolemmal na+, k+-activated adenosine triphosphatase (na+, k+-atpase) in carp heart, which showed a negative force-frequency relationship, and in guinea-pig heart, which has a positive relationship. 2. in ventricular muscle preparations isolated from carp heart, strophanthidin increased d ...19872822523
red cell function of carp (cyprinus carpio) in acute hypoxia.the red cell function of carp, subjected to acute hypoxia, was studied. during hypoxic exposure red cell water content, sodium and chloride concentration and ph increased, whereas plasma sodium and chloride concentrations decreased. treatment of the animals with a beta-adrenergic antagonist, propranolol, before the onset of hypoxia, largely inhibited the increase in red cell water content, diminished the changes in red cell ph and prevented the changes in red cell and plasma sodium concentration ...19872822469
studies on the linkage between spin equilibria and protein structure in carp ferric hemoglobin.the effects of protein conformation on the spin-state equilibria of several derivatives of carp hemoglobin have been examined. this has been done by measuring the ph dependence of the paramagnetic susceptibilities of these derivatives in the presence and absence of inositol hexakisphosphate, p6-inositol. in all cases the addition of p6-inositol at low ph and the lowering of the ph in the presence of p6-inositol shift the spin-state equilibrium in favor of the high-spin electronic configuration. ...19872822419
the 18p- syndrome. report of five cases.five children, three males and two females were found to have a short arm deletion of chromosome 18. four of them display some of the typical features of this syndrome: microcephaly, round face, hypertelorism, broad-based nose, "carp-mouth", microrethrognathia, pterygium colli, dysplastic and low set ears, clinodactily, failure to grow, muscular hypotony and mental retardation. different hypotheses are discussed in order to explain the variable phenotypical expression of the 18 p-syndrome.19892817777
development of "csaba cells" in experimentally infected cyprinus carpio.the development of "csaba cells" in the blood of experimentally infected common carp showed the various different stages as previously described in the literature. most frequent were stage 1 and later stage 5 cells. 3 subsequent passages were successful.19892817508
a functional explanation for a sequence of developmental events in the carp. the absence of gills in early larvae.the animal body is composed of structural elements which can be characterized by their size, position, external form, internal structure and their participation in functions. their presence is taken for granted. the ontogenetic sequence of development of functional systems in fish shows an early absence of gills although effective and fast suction feeding movements are already found in 6 mm larvae. calculations of the oxygen diffusion boundary layer in these larvae which would be present when or ...19892816523
some observations on the narcotizing ability of electric currents on the common carp cyprinus carpio.some effects of alternating current electronarcosis and of rectified current electronarcosis on c. carpio were investigated. in all instances recovery from narcosis was accompanied by convulsive spasms. haemorrhaging of the gills was also observed to occur. carp do not appear to be suitable candidates for electronarcosis.19892812707
[a dynamic model of the interaction of cones with bright horizontal cells in the carp retina].the dynamic model showing interaction between cones and l-type horizontal cells in the carp retina is discussed. the model is based on the intracellular recording of spectral responses of horizontal cells. the model equations describe the contribution of ionic channels to responses of cones and horizontal cells. the model permits predicting responses of photopic l-cells as a function of time.20092812137
similarity of ion dependence of odorant responses between lipid bilayer and olfactory system.in a previous paper (nomura and kurihara (1987) biochemistry 26, 6135-6140), we demonstrated that the lipid bilayer membranes exhibit membrane potential changes in response to various odorants similarly to olfactory cells. the present study demonstrated that ion dependence of the responses of the lipid membranes to odorants is related to that in the carp olfactory system in the following point. (1) the responses to odorants diminished at low concentrations of salts and recovered upon addition of ...19892804055
the parasitemia of cloned trypanoplasma borreli laveran and mesnil, 1901, in laboratory-infected common carp (cyprinus carpio l.).the course of parasitemia of cloned trypanoplasma borreli in laboratory-infected common carp was investigated. in 25-42-g carp kept at 20 c, the prepatent period was 8 days; after a phase of exponential growth, the parasitemia peaked at day 39 postinjection (pi) at a level of about 10(3) t. borreli/microliters blood. this maximum was followed by a chronic phase of about 6 wk with large numbers of t. borreli. at 20 wk pi, t. borreli was absent in infected carp. in 2.2-g carp kept at 20 c, the pre ...19892795371
neuroglia in the optic tectum of teleosts.the morphology of the neuroglial cells of the optic tectum of cyprinid teleosts (cyprinus carpio and barbus bocagei) was analyzed using two variants of the golgi method (colonnier and meyer) and electron microscopy. although the description of the results is based on the three classical neuroglial types (ependymocytes, astrocytes and oligodendrocytes), their analysis shows that the differentiation between astrocytes and oligodendrocytes is complex. owing to the occurrence of numerous elements wi ...19892794486
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