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monoclonal antibodies directed against the calcium binding protein parvalbumin.we have produced twelve monoclonal antibodies (mcab) against carp-ii parvalbumin. three of them, designated 235, 239, 267 recognize determinants conserved in fish, chicken, mouse, rat, monkey and human parvalbumin. we show their use in the qualitative detection of parvalbumin (pv) by immunohistochemistry, in the quantitation of parvalbumin by radioimmunoassay and in the detection of parvalbumin on immunoblots.19883383226
characteristics of bipolar-bipolar coupling in the carp retina.on and off bipolar cells were identified in the light-adapted carp retina by means of intracellular recording and lucifer yellow dye injection. the receptive field centers, determined by measuring the response amplitudes obtained by centered spots of different diameters, were 0.3-1.0 mm for on bipolar cells and 0.3-0.4 mm for off bipolar cells. these central receptive field values were much larger than the dendritic field diameters measured by histological methods. simultaneous intracellular rec ...19883373179
[a case of acute renal failure after eating the raw gallbladder of a carp]. 19883373090
isolation and properties of the pituitary gonadotropin from silver carp (hypophthalmichthys molitrix).the gonadotropin of silver carp (scgth) was purified from the pituitary glands in a yield of 8.4 mg/g through extraction with 6% ammonium acetate (ph 5.1)-40% ethanol followed by 80% ethanol precipitation, deae-cellulose chromatography and gel filtration. the molecular weight of scgth is about 32,000 as estimated by gel filtration. leucine and serine were the two n-termini of scgth. the amino acid composition of scgth is rich in aspartic acid/asparagine, glutamic acid/glutamine, valine, proline ...19883366548
gonadotropin content of the pituitary gland of gonadal tumor-bearing common carp x goldfish hybrids from the great lakes, as assessed by bioassay and radioimmunoassay.the hybrids of carp (cyprinus carpio) and goldfish (carassius auratus) collected from the lower great lakes between 1978 and 1981 exhibited epizootics of gonadal neoplasm which were rare in the parental species. the pituitary glands of hybrids were, on average, 2.5 times larger than that of carp of similar body size. histologically, the hypertrophy was due to hyperplasia of proximal pars distalis basophils (the presumptive gonadotropes). using a carp gonadotropin (gth) radioimmunoassay it was fo ...19883366360
light-dependent dynamics of gap junctions between horizontal cells in the retina of the crucian carp.the dynamics of gap junctions between outer horizontal cells or their axon terminals in the retina of the crucian carp were investigated during light and dark adaptation by use of ultrathin-section and freeze-fracture electron microscopy. light adaptation was induced by red light, while dark adaptation took place under ambient dark conditions. the two principal findings were: (1) the density of connexons within an observed gap junction is high in dark-adapted retina, and low in light-adapted ret ...19883365755
microridges of oral mucosal epithelium in carp, cyprinus carpio.the surface of carp oral mucosa is characterized by various patterns of microridges about 0.3 micron wide, 0.1 micron high, and of various lengths. to elucidate the derivation and function of these microridges, the oral epithelium was examined by light- and electron microscopy. microridges were present only on the surfaces of the superficial cells. therefore, microridges on renewed superficial cells have been discarded, and the various patterns of microridges found on the cell surface appear to ...20123365750
identification of hypocalcin (teleocalcin) isolated from trout stannius corpuscles.we have isolated and purified a glycoprotein from the corpuscles of stannius (cs) of trout, which we consider hypocalcin (also called teleocalcin), the major hypocalcemic hormone of fish. this product is present in relatively large amounts in the cs of several species (i.e., european eel, tilapia, goldfish, and carp). hypocalcin is typically released from the cs in response to an experimentally induced increase of the blood calcium concentration. ultrastructural observations show that after this ...19883360288
regional difference in the dendritic morphology of dopamine cells in carp retina.the dendritic morphology of dopamine (da) cells in the inner plexiform layer of the retina of carp (body length, ca. 33 cm) was investigated by identifying their fluorescent cell bodies in isolated, aldehyde-fixed flat-mounts and injecting them iontophoretically with lucifer yellow ch under microscopic control. attention was paid to clarifying regional differences in their dendritic morphology. in the marginal zone within 0.25 mm from the retinal edge, the density of da cells was extremely high ...19883359333
[reaction of the carp cyprinus carpio l. at different periods of its ontogeny to the action of organophosphorus compounds].effect of some organic phosphorous compounds on the carp cyprinus carpio l. in consecutive stages of its development has been studied. the least and most vulnerable stages in carp's development under the action of these compounds have been identified. the gravity of disorders in fish organism on early stages and the degree of pathological changes induced by organic phosphorous compounds have been shown. embryotoxic and gonadotoxic effect of these compounds has been found.19883358968
effects of ligand size on ph and organic phosphate-dependent affinity changes in carp hemoglobin as measured by isonitrile binding.the equilibria of the binding of methyl and ethyl isonitrile to carp hemoglobin have been measured at three ph values in the presence and absence of inositol hexaphosphate. the binding of methyl isonitrile is characterized by a higher overall dissociation constant, c1/2, and a higher hill coefficient, n, than that of the ethyl derivative. the former is consistent with the greater hydrophobicity of ethyl isonitrile, and the latter is probably due to a greater intrinsic difference or heterogeneity ...19883358940
species differences in 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity and biotransformation in fish.rainbow trout, yellow perch, carp, bluegill, largemouth bass, and bullhead were treated with graded doses of 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd; 1, 5, 25, or 125 micrograms/kg) or vehicle, ip. the lethal potency of tcdd tended to be greater in yellow perch, carp, and bullhead than in the other three species (ld50 80 days post-treatment, 3-5 versus 10-16 micrograms/kg, respectively). all species treated with the highest dose of tcdd (125 micrograms/kg) displayed a latency period of 1-4 wee ...19883356307
temperature effects on ph of mitochondria isolated from carp red muscle.mitochondria isolated from red muscle of carp (cyprinus carpio) were used to investigate the effects of temperature and extramitochondrial ph (phe) on the mitochondrial ph gradient and respiratory properties. mitochondria from animals acclimated to 10 degrees c were isolated and incubated in kcl-based media with 0.2 mm lauroylcarnitine (c-12) as substrate. maximal respiratory control ratios (rcr = state 3/state 4) were 16-18 between ph 6.7 and 7.4 at 10 degrees c; rcr values were 9-12 between ph ...19883354709
cutaneous tumor of northern pike, esox lucius l. ii. expression of surface and cytoplasmic immunoglobulins and alpha-naphthyl acetate esterase by mononuclear cells from lymphoid tissues and tumor.rabbit anti-pike igm antibodies showed that up to 95% of mononuclear (mn) cells isolated on ficoll-isopaque gradients from peripheral blood, spleen, and head kidney of northern pikes carrying cutaneous tumors were surface (sig) and cytoplasmic (cig) immunoglobulin positive by indirect immunofluorescence. on the contrary, rabbit anti-winter flounder igm antibodies gave totally negative results. monoclonal anti-carp igm antibodies stained approximately 40-56% of peripheral blood, splenic, and head ...19883350193
adaptation of intestinal morphology in the temperature-acclimated carp, cyprinus carpio l.the effects of temperature and photoperiod acclimation upon the morphology of carp intestinal mucosa have been studied using morphometric techniques. carp intestine showed an absence of anatomical regionalisation. there was a gradual reduction in the dimensions of villi along the tract. the decrease in the dimensions of the villi was greatest in the anterior half. temperature acclimation had no effect on intestinal-somatic indices. acclimation to 10 degrees c or 30 degrees c resulted in large di ...19883345555
morphology of physiologically identified mitral cells in the carp olfactory bulb: a light microscopic study after intracellular staining with horseradish peroxidase.physiologically identified mitral cells in the carp olfactory bulb were stained by intracellular injection of horseradish peroxidase in order to study the morphology in detail. the somata were fusiform, elongated, oval, triangular, or irregular. the mean diameters of the somata were 30 microns x 14 microns. two to five thick dendrites arose from the somata and frequently gave off branches to form glomerular tufts. the dendrites extended less than 400 microns; the dendritic field of single mitral ...19883343400
a study of the hydration and thermodynamics of warm-water and cold-water fish collagens.the hydrated volumes, vh, of collagens extracted from various fish species were calculated by using the simha-einstein equation, and it was found that the hydration of warm-water fish collagen is greater than that of cold-water fish collagen (halibut). although the intrinsic viscosities of warm-water fish (bigeye-tuna, carp and catfish) collagens are almost the same, the hydrated volume of bigeye-tuna collagen is approx. 1.5 and 3 times those of carp and catfish collagens respectively. the exten ...19883342002
properties of odour-binding glycoproteins from rat olfactory epithelium.the specific membrane glycoproteins with high affinity for camphor and decanal were isolated from rat olfactory epithelium. antibodies to these glycoproteins inhibited both the electroolfactogram and the binding of odorants. the enzyme immunoassay has shown these glycoproteins to be present in the olfactory epithelium of rat, mouse, guinea-pig and hamster but not in that of frog and carp. the molecular mass of the odour-binding glycoproteins from rat olfactory epithelium solubilized by triton x- ...19883337807
gabaergic inhibition on dopamine cells of the fish retina: a [3h]dopamine release study with isolated fractions.inner retinal cells including dopamine (da) cells were isolated and fractionated from the carp (cyprinus carpio) retina by an enzyme cell dissociation and metrizamide gradient centrifugation method. when gamma-aminobutyric acid (gaba) antagonists (bicuculline and picrotoxin) were added into the perfusate over such a cell fraction, they stimulated the release of [3h]da which had been preloaded in the cell fraction. the action of gaba antagonists was dose and ca2+ dependent. their minimal effectiv ...19883335837
interaction of parvalbumin of pike ii with calcium and terbium ions.fluorimetric titrations of parvalbumin ii (pi 4.2) of pike (pike ii) with ca2+ and tb3+ show the cd and ef binding sites to be non-equivalent. the intrinsic binding constants of the strong and the weak sites obtained for ca2+ are: ksca = 1.6.10(8) m-1; kwca = 6.6.10(5) m-1. differences of the order of 100% were encountered between the tb3+ binding constants obtained with four different versions of titration. their average values are: kstb = 1.9.10(11) m-1; kwtb = 1.0.10(7) m-1. the distances of ...19883334854
[sphaerospora renicola (myxosporidia), the agent of protozoal swim bladder inflammation in young carp (renicola sphaerosporosis)]. 19873328529
primate specific sialoglycoprotein of sperm head plasma membrane defined by an anti-carbohydrate monoclonal antibody.a sialoglycoprotein, an integral component of the head plasma membrane of human spermatozoa, is recognized by the a-hs 1a.1 monoclonal antibody. the antigenicity is associated with the sugar moiety since: a) trypsin digestion did not affect the antigenic determinant; b) pretreatment of the cells with beta-glucosidase, alpha-mannosidase and neuraminidase completely abolished antibody binding. endoglycosidase d and glycopeptidase f were inactive. the a-hs 1a.1 did not recognize a variety of blood- ...19873317219
cloning and sequencing of a carp beta s-crystallin cdna.the mrnas were extracted from common carp (cyprinus carpio) lenses, purified, reverse transcribed, dc tailed and cloned into escherichia coli with pbr322 as vector. the cloning efficiency was around 1 x 10(7) colonies per micrograms of mrna. a clone (pc20) was found by hybrid-arrested to contain the cdna related to carp crystallins. however, comparison of the derived amino-acid sequence with bovine gamma-ii and beta s-crystallins indicates that this carp crystallin sequence resembles closely the ...19873307924
urotensin i-like immunoreactivity in amacrine cells of the goldfish retina.urotensin i-like immunoreactivity (uili), in different localization from that of corticotropin releasing factor-like immunoreactivity (crfli), in the goldfish retina has been demonstrated by means of radioimmunoassay, high-performance liquid chromatography (hplc) and immunohistochemistry. radioimmunoassay showed 350 +/- 40 pg/mg prot. of uili in goldfish retina extracts. the immunoreactive material present in the retina was also characterized by reversed phase hplc. some of the uili co-eluted wi ...20113295600
morphological and immunohistochemical studies of juxtaglomerular cells in the carp, cyprinus carpio. 19873295351
effects of [d-arg6, trp7, leu8, pro9net]-luteinizing hormone-releasing hormone (sgnrh-a) and [d-ala6, pro9net]-luteinizing hormone-releasing hormone (lhrh-a), in combination with pimozide or domperidone, on gonadotropin release and ovulation in the chinese loach and common carp.the effects of lhrh-a and sgnrh-a alone and in combination with the dopamine receptor antagonists pimozide (pim) and domperidone (dom) on stimulation of gonadotropin (gth) secretion and ovulation in chinese loach (paramisgurnus dabryanus) and common carp (cyprinus carpio) were studied. when tested in the absence of a dopamine receptor antagonist, sgnrh-a was about 10-fold more potent than lhrh-a in stimulating gth secretion in loach, whereas these peptides were of similar effectiveness in common ...19883282976
the fetal phenotype of the 18p-syndrome. report of a male fetus at twenty-one weeks.morphological and cytogenetic findings in a male fetus at 21 weeks gestation after prenatally detected monosomy 18p are reported. the fetus displayed dysmorphic features resembling the 18p-syndrome, such as decreased head circumference, slightly receding forehead, hypertelorism, epicanthus, horizontal palpebral fissures, depressed nasal bridge, long philtrum, carp mouth, irregular crenated maxillar alveolar ridge, retrognathia, lowset dysplastic ears with posterior rotation, edema of neck, hands ...19883281573
[study on ldh isoenzymes of hybrid yue-carp and its parents]. 19883273660
adaptational change in cone-mediated electroretinogram in human and carp. 19883265993
comparative studies on the structure of biliary immunoglobulins of some avian species. ii. antigenic properties of the biliary immunoglobulins of chicken, turkey, duck and goose.studies on the immunological cross-reactivity of the igs+ from the bile of chicken and turkey (order galliformes) as well as duck and goose (order anseriformes) to igs with antigenic known properties carried out by a series of ria. various class-specific anti-ig sera produced in rabbits, guinea-pigs and mice were used. the following results were obtained: (1) a high extent of antigenic relationship between the chicken and turkey biliary igs was demonstrated by a radiobinding system consisting of ...19883260198
the protein sequence homology of gamma-crystallins among major vertebrate classes and their dna sequence homology to heat-shock protein genes.a systematic characterization of lens crystallins from five major classes of vertebrates was carried out by exclusion gel filtration, cation-exchange chromatography and n-terminal sequence determination. all crystallin fractions except that of gamma-crystallin were found to be n-terminally blocked. gamma-crystallin is present in major classes of vertebrates except the bird, showing none, or decreased amounts, of this protein in chicken and duck lenses, respectively. n-terminal sequence analysis ...19883255376
effects of various oxygen concentrations on antioxidant enzymes and the quantity of tissue phospholipid fatty acids in the carp.analyses were made of the phsopholipid fatty acids and the antioxidant enzymes in the carp (cyprinus carpio morpha) at three different oxygen concentrations, corresponding to hyperoxia, hypoxia and anoxia. variations of the oxygen concentration were found to influence the quantities of phsopholipid fatty acids, as well as the antioxidant enzyme activities. in hyperoxia and hypoxia the amount of polyunsaturated fatty acids in carp liver was higher than in anoxia, but in other tissues there was no ...19883254009
a comparative trial of garlic, its extract and ammonium-potassium tartrate as anthelmintics in carp.while fish chemotherapy is not a common practice in méxico, folk medicine is usually well accepted by fish farmers. empirical knowledge suggested that garlic should have anthelmintic properties. various trials were carried out in cyprinus carpio infested with capillaria sp., using minced garlic and its extracts. an empirical concentration of 200 mg/l of minced garlic showed the greatest activity against the above mentioned worms (100%). the hexane extract in equivalent amounts showed a 75% effec ...19883253490
modulation of cone horizontal cell activity in the teleost fish retina. i. effects of prolonged darkness and background illumination on light responsiveness.the effects of prolonged (greater than 2 hr) darkness and background illumination on the light responsiveness of cone horizontal cells were examined in isolated, superfused white perch retinas. in retinas from fish maintained in complete darkness for more than 2 hr, cone horizontal cells had a resting membrane potential of about -18 mv, and they generated only slow, low-amplitude (3-4 mv) responses even when stimulated with bright flashes. following the presentation of dim background light, the ...19883249224
[a morphometric analysis of erythrocytes and the level of antiviral antibodies in the carp blood at the final stage of the infectious process]. 20123248597
primary structures of carp gonadotropin subunits deduced from cdna nucleotide sequences.the alpha and beta subunits of carp gonadotropin (cgth) were isolated by high performance liquid chromatography. they were identified to be the subunits of cgth by bioassay and by partial n-terminal amino acid sequence analysis. to elucidate the complete primary structures of the alpha and beta subunits of cgth, cdna cloning technique was employed. the alpha and beta subunits consist of 95 and 115 amino acid residues, respectively. homology of the alpha subunit of cgth to those of mammalian gth ...19883246480
comparative histochemistry of the mucoproteic cells of the hypophysis from rhamdia hilarii, hypostomus punctatus, prochilodus scrofa and cyprinus carpio (teleostei). immunohistochemical identification of the gonadotropic cells.adenohypophyseal cells showing positive histochemical reactions for mucosubstances were classified as type i-iv in hypostomus (plecostomus) punctatus (loricariidae), rhamdia hilarii (pimelolidae), prochilodus scrofa (prochilodontidae) and cyprinus carpio (cyprinidae) according to cell shape, size, cytological characteristics and adenohypophyseal distribution. cell types i and ii are common to the four species, with each cell type showing very similar cytological and histochemical characteristics ...19883242625
[the effect of temperature acclimation on nadp-dependent dehydrogenase activity in the carp liver and various mechanisms of its regulation].acclimation of carp both to the temperature fall (from 20 to 5 degrees c) and rise (from 20 to 30 degrees c) induces an increase in activity of cytoplasmic liver nadph-generating enzymes--glucose-6-phosphate dehydrogenase (g6pdg) and malic-enzyme (me) 6-phosphogluconate dehydrogenase (6pgdg) and nadp-isocitrate dehydrogenase (nadp-idg) activities are unchanged. actinomycin d does not prevent cold activation of g6pdg but blocks activation of me. "warm" g6pdg has minimal km value for glucose-6-pho ...20153238795
xenobiotic metabolizing enzyme systems in test fish. iii. comparative studies of liver cytosolic glutathione s-transferases.the reduced glutathione (gsh)-conjugating capacities of the livers of trout, carp, zebra fish, guppy, and bluegill have been evaluated with several substrates preferentially metabolized by the major gsh-transferase isozymes. carp and zebra fish lack gsh-transferase activity with 1,2-epoxy-3-(p-nitrophenoxy)propane. guppy is characterized by the highest activities with 1-chloro-2,4-dinitrobenzene and the absence of activity toward trans-4-phenyl-3-buten-2-one. enzyme activities with the other sub ...19883234293
purification and some properties of glyceraldehyde-3-phosphate dehydrogenase from carp muscle.glyceraldehyde-3-phosphate dehydrogenase was purified from carp white muscle. on cm-sephadex chromatography two well separated active peaks were obtained. both of them show a single protein band on gel electrophoresis and have the same molecular and kinetic properties; they differ only by the amount of bound nad, the enzyme in the second peak being coenzyme-free. significant differences were observed between the properties of carp and pig muscle enzymes. glyceraldehyde-3-phosphate dehydrogenase ...19883232465
effects of dopamine on spatial properties of horizontal cell responses in the carp retina. 19883231368
[residues of active substances after consumption of contaminated food: status report on the evaluation of residues with examples of two drugs].pharmacokinetic models are presented for the computation of time courses of blood levels of drugs in man following the consumption of contaminated food. mathematically, two linear systems of differential equations are set up for the donor organism (e.g., trout) and for the recipient, (e.g., man), where the first system generates the initial conditions for the second. models of this kind are applied to the transfer of chloramphenicol to man via carp and trout (which had previously been administer ...19883223091
trace element residues in bluegills and common carp from the lower san joaquin river, california, and its tributaries.whole-body samples of bluegills (lepomis macrochirus) and common carp (cyprinus carpio) from the san joaquin river and two tributaries (merced river and salt slough) were analyzed to determine if the concentrations of any of nine elements were elevated as a result of exposure of the fish to agricultural subsurface (tile) drainage water. highest concentrations (micrograms/g dry weight) detected were as follows (first number in each pair applies to bluegills and the second to carp): arsenic (as), ...19883222692
a comparative study of aldehyde dehydrogenase and alcohol dehydrogenase activities in crucian carp and three other vertebrates: apparent adaptations to ethanol production.in the final step of the pathway producing ethanol in anoxic crucian carp (carassius carassius l.), acetaldehyde is reduced to ethanol by alcohol dehydrogenase. the presence of aldehyde dehydrogenase in the tissues responsible for ethanol production could cause an undesired oxidation of acetaldehyde to acetate coupled with a reduction of nad+ to nadh. moreover, acetaldehyde could competitively inhibit the oxidation of reactive biogenic aldehydes. in the present study, the distribution of aldehyd ...19883220990
seasonal variations of glucose and triiodothyronine concentrations in serum of carp (cyprinus carpio l).thyroid hormone and glucose serum concentrations (sc) of carps (cyprinus carpio l) have been monitored at weekly intervals throughout the year. t4 and rt3 concentrations were always below the limit of detection of the assays applied. t3 and glucose mean sc showed seasonal variations. highest t3 sc were reached in summer with a peak value in august and lowest values in winter with a maximum in december. glucose sc have been found to be highest in winter and lowest in summer. statistical analysis ...19883220447
[rhythmic activity of the olfactory bulb of the carp under nembutal anesthesia].the effect of nembutal intraperitoneal injections upon the induced waves and orthodromic potentials of the olfactory bulb (ob) induced by odor stimulation and paired electric stimuli of the olfactory nerve was examined in immobilized by diplacine carp (cyprinus carpio l.). the nembutal anesthesia resulted in a gradual decrease in frequency of induced waves tested by orthodromic response and amplitude of spontaneous electric activity ob ob. the changes found under nembutal anesthesia in evoked el ...19883219368
ultrastructural analysis of leucocyte interaction with tumour targets in a teleost, cyprinus carpio l.the first step in natural killer activity, target cell binding, was investigated ultrastructurally in order to identify presumptive cytotoxic cells. after incubation of unfractioned peripheral blood cells of the carp, cyprinus carpio l., with hela tumour cells as targets, regular conjugate forming was observed. targets were surrounded by cells bearing features either of lymphocyte or monocyte/macrophage types. cells with the usual lymphocytic characteristics contained acid phosphatase positive g ...19883208960
dopamine cells and rod bipolar cells contain protein kinase c-like immunoreactivity in some vertebrate retinas.the localization of cells immunoreactive to a monoclonal antibody against protein kinase c (pkc) and to polyclonal antibodies against tyrosine hydroxylase (th) was investigated in the retina of fish (carp, goldfish, dace and catfish), frog, turtle, chick and some mammalians (guinea pig, rat, cat and rabbit) by means of fluorescence microscopy. pkc-like immunoreactivity was found in dopamine (da) or th-like immunoreactive (ir) cells in all the species examined and also in rod bipolar cells in the ...19883205402
localisation and identification of melanocyte-stimulating hormones in the fish brain.the existence of melanocyte-stimulating hormone (msh) in fish brains was investigated by a range of techniques: radioimmunoassay, hplc, bioassay, and immunocytochemistry. immunoreactive alpha msh (ir alpha msh) was detected by radioimmunoassay in all regions of carp and trout brains, with the highest concentration in the basal hypothalamus. in trout, ir alpha msh cell bodies were located by immunocytochemistry only periventricularly, in the medial basal hypothalamus near the third ventricle, whe ...19883203871
in vitro effectiveness of estradiol-17 beta, androgens, corticosteroids, progesterone, and other pregnene derivatives on germinal vesicle breakdown in oocytes of the exotic common carp cyprinus carpio (l.). 19883198160
daily cycles in plasma melatonin levels under long or short photoperiod in the common carp, cyprinus carpio.a radioimmunoassay (ria) for plasma melatonin (mlt) was simplified for use with the common carp (cyprinus carpio). plasma was partially purified with sep-pak c18 cartridge before ria. the inhibition curves for the sep-pak c18 fraction from the plasma of carp, goldfish (carassius auratus), yellow tail (seriola quinqueradiata), japanese eel (anguilla japonica), bora (mugil cephalus cephalus), kisu (sillago japonica), ishigarei (kareius bicoloratus), and shimaisaki (rhyncopelates oxyrhynchus) were ...19883197949
development of sensitivity to maturation-inducing steroids in the oocytes of the daily spawning teleost, the kisu sillago japonica.the responsiveness of oocytes to 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17 alpha,20 beta-dioh-p), 17 alpha-hydroxyprogesterone (17 alpha-oh-p), and silver carp gonadotropin (gth) was examined in vitro at seven different times of the day (0100, 0500, 0800, 1000, 1300, 1700, 2100 hr) in a daily spawning teleost, the kisu sillago japonica. 17 alpha,20 beta-dioh-p, 17 alpha-oh-p, and gth were effective in inducing germinal vesicle breakdown (gvbd) and ovulation in kisu oocytes. however, the ooc ...19883197947
interactions between human and carp (cyprimus carpio) low density lipoproteins (ldl) and ldl receptors.1. we have compared the concentration and chemical composition of carp and human plasma lipoproteins and studied their interaction with human fibroblast ldl receptors. 2. the main lipoproteins in carp are of high density (hdl) in contrast to low density lipoproteins (ldl) in human. 3. carp lipoproteins are devoid of apolipoprotein (apo) e, a major ligand for interaction with ldl receptors in mammals. 4. carp very low density lipoproteins (vldl) and ldl but not hdl nor apoa-i cross react with hum ...19883197401
[slow potentials of the olfactory bulb in the carp].the slow negative potentials evoked in carp olfactory bulb (ob) by some odorants and slow positive potentials evoked by nonspecific irritation (water stream, nacl solutions) of olfactory epithelium have been studied. the slow potentials of both types were not inverted in deep layers of ob and were resistant to blockade of synaptic transmission by manganese ions. the negative slow potentials were not also affected by hypoxia and associated with local increase of ob tissue resistance. positive slo ...20113196780
the effect of temperature on immunoreactive glucagon plasma level in carp cyprinus carpio.plasma immunoreactive glucagon levels (irg), plasma glucose levels and brain and liver glycogen concentrations were analyzed in carp (adapted to 15 degrees c) subjected to short-term temperature changes (1.6 or 11 h, at 5 degrees c or 28 degrees c, respectively) and to long-term temperature changes (21 months at 28 degrees c). the high temperature (28 degrees c) produced significant increases in irg in both short and long-term experiments. brain glycogen also decreased in both experiments wherea ...19883194572
the complete amino acid sequences of beta-subunits of two distinct chum salmon gths.the complete amino acid sequences of beta-subunits of chum salmon (oncorhynchus keta) gonadotropin (gth) i and gth ii were determined. both gth i beta and gth ii beta were treated with neuraminidase, and then reduced and carboxymethylated. both of the modified subunits were digested separately with several endopeptidases. the resulting fragments were purified by reversed-phase high-performance liquid chromatography and subjected to sequence analysis by manual edman degradation. gth i beta was a ...19883192067
carp gamma-crystallins with high methionine content: cloning and sequencing of the complementary dna.the nucleotide sequences of gamma-crystallin cdnas cloned from the common carp (cyprinus carpio) have been determined. the amino-acid sequences derived consist of two polypeptides with 177 and 172 amino-acid residues for gamma-m1 and gamma-m2, respectively. they exhibit unusually high methionine contents: 12.4% for gamma-m1 and 14% for gamma-m2. comparison of both fish gamma-crystallins with bovine gamma-ii crystallin reveals that they are similar in structure. the striking features of both fish ...19883191133
[characteristics of carbohydrate metabolism in skeletal muscles of the sea carp diplodus annularis l].the activity of phosphorylase and glycogen level in white and red skeletal muscles of d. annularis was investigated after two minutes after swimming with the rate of 1.8 m/s. the indicated muscular load decreased the glycogen level in the white muscles by 70%. it has been found that changes in the carbohydrate metabolism of the d. annularis red muscles were negligible.20153188263
[the seasonal dynamics of sphaerospora renicola (myxosporidia) in carp (osteichthyes)].we observed 2 different types of the course of incidence of sporogonic phase and blood phase. type i shows in both phases a maximum in the first summer, a minimum in winter or autumn and a second maximum between late autumn and second summer. in this case the sporulation was especially distinct with the first maximum. type ii starts in the first summer with the blood phase without occurrence of the sporogonic phase (and the swim-bladder phase), which appears only from autumn until the second sum ...19883177937
comparative biotransformation of molinate (ordram) in the white sturgeon (acipenser transmontanus) and common carp (cyprinus carpio).1. juvenile white sturgeon (acipenser transmontanus) and common carp (cyprinus carpio) were exposed to 100 micrograms/l [ring-14c]molinate in a flow-through metabolism system. exposure was in three phases: acclimation (2 h), uptake (24 h), and depuration (24 h). 2. excreted metabolites were collected on a macroreticular resin, and retained metabolites were extracted from homogenized fish tissue. identification and quantification was by h.p.l.c. cochromatography (gradient conditions) and determin ...19883176521
pharmacokinetics of ciprofloxacin in carp, african catfish and rainbow trout.the plasma disposition of ciprofloxacin was studied in carp, african catfish and trout after intravenous (iv) and intramuscular (im) administration at a dose rate of 15 mg/kg. pharmacokinetic analysis of iv data showed that ciprofloxacin was well distributed (distribution volume vd(area): 3.08-5.59 litre/kg) and exhibited a similar elimination half-life of about 14 h in these 3 fish species. after im administration to carp and trout a rapid absorption was noticed; the maximum ciprofloxacin plasm ...19883176297
nucleotide sequence of carp prolactin cdna. 19883174460
unknown syndrome: abnormal facies, hypothyroidism, and severe retardation: a second patient.in the november 1987 issue of this journal, young and simpson presented a female infant with abnormal facies (microcephaly, blepharophimosis, small, low set, posteriorly rotated ears, bulbous nose, carp shaped mouth, and micrognathia), congenital heart abnormalities (large atrial and ventricular septal defects), congenital hypothyroidism, and severe global retardation. we have observed a male newborn with a similar pattern of malformations (figs. 1 and 2).19883172145
dna:dna reassociation analysis of aeromonas salmonicida.dna from 26 aeromonas salmonicida strains, namely 11 'typical' and 15 so-called 'atypical' strains, was used to assess the taxonomic relatedness within the species. the genomes were characterized by determination of dna base composition, dna:dna reassociation, calculation of sequence divergence following reassociation, and by genome size estimations. by comparison with dna obtained from controls and the aeromonas hydrophila group, a. salmonicida strains were determined to be correctly placed wit ...19883171541
effect of manganese ions on the immune response of carp (cyprinus carpio) against yersinia ruckeri.the effect of manganese ions mn2+ (mn cl2) on hematocrit and the antibody response of carp (cyprinus carpio) was investigated after exposure of fish to contaminated water for 2.5 months. contamination of water with 50 mg/l mn2+ did not decreased antibody production against yersinia ruckeri (measured by agglutination) but significantly decreased hematocrit. the levels of manganese in lymphoid organs (spleen and kidney) increased progressively during the experiment. the effect of manganese in vitr ...19883169354
fish lymphokines: the production of growth factors in supernatants of mitogen-stimulated pronephros cells of carp (cyprinus carpio). 19853161628
changes in tension generation and atpase activity in skinned muscle fibres of the carp following temperature acclimation.common carp (cyprinus carpio l.) were acclimated to either 7 degrees c or 23 degrees c for 1-2 months. skinned fibre preparations were isolated from the white myotomal muscle, and atpase activity measured during maximal isometric contractions. at 7 degrees c, fibres from the cold acclimated fish were found to generate more force than those from warm acclimated fish (123.1 and 97.2kn m-2 respectively), and more "work" (force x time integral) was obtained for each atp hydrolysed. atp turnover per ...19853160006
localization of immunoreactive synthetic atrial natriuretic factor (anf) in the heart of various animal species.the localization of two synthetic fragments of the c-terminal portion of atrial natriuretic factor: arg 101-tyr 126 which displays full biological activity and leu 94-arg 109 which is completely devoid of biological activity, has been investigated by immunohisto- and immunocytochemical methods in the heart of mammals (rat, mouse, guinea pig, hamster, rabbit, cat, dog, man) and nonmammalian vertebrates toad (bufo marinus), frog (rana catesbeiana), fish (cyprinus carpio, puntius schwanenfeldi, cic ...19853158698
electron probe x-ray microanalysis of post-tetanic ca2+ and mg2+ movements across the sarcoplasmic reticulum in situ.ca2+ and mg2+ movements across the sarcoplasmic reticulum (sr) of frog skeletal muscle fibers were measured in situ by electron probe microanalysis of muscles rapidly frozen following a tetanus. at 400 ms following a 1.2-s tetanus at room temperature, the force had relaxed to base-line, and 0.3 mmol of ca2+/liter of cytoplasmic h2o had been pumped by the sr, indicating that the in situ pumping of the sr ca-atpase is sufficiently high to account for the removal of ca2+ from the ca2+-specific site ...19853158652
[monoclonal antibodies against differentiation antigens of carp thymocytes (cyprinus carpio l.)].using the cell fusion technique we have produced monoclonal antibodies directed against carp thymocytes. the monoclonals revealed a different ability for binding to carp lymphocytes of various origin. the antibodies reacting only with lymphocytes seem to react with a membrane structure of a molecular weight of 200 000 to 250 000 daltons.19853158176
temperature acclimatization of the carp. cellular and molecular aspects of the compensatory response. 19883154855
nucleotide sequence of chicken myb proto-oncogene promoter region: detection of an evolutionarily conserved element.the nucleotide sequence of the chicken myb proto-oncogene putative promoter region was determined and compared with the corresponding sequence of the mouse c-myb gene (1). 118 bp upstream from the initiation codon suggested by gerondakis and bishop (2) for the chicken c-myb protein, a 124-bp-long conserved element was found (92% identity in chicken and mouse sequences). sequences homologous to this element were detected on southern blots of restricted genomic dnas from mouse, man, lizard, frog, ...19883145493
effect of adrenaline and blood gas conditions on red cell volume and intra-erythrocytic electrolytes in the carp, cyprinus carpio.1. carp, when subjected to air stress in vivo, developed a hypoxaemia associated with an acidosis due to elevation of pco2 and arterial lactate content. adrenaline and noradrenaline levels rose markedly, and the mean corpuscular haemoglobin concentration (mchc in mmol hb l-1 red blood cells) decreased, indicating swelling of the erythrocytes. 2. no effect of adrenaline could be observed in vitro after equilibration at normal ph and oxygenation level. 3. simulation of air stress in vitro, by usin ...19883145321
identification and immunocytochemical localization of two different carbonic anhydrase isoenzymes in teleostean fish erythrocytes and gill epithelia.carbonic anhydrase was purified from the gills (cab) of the rainbow trout salmo gairdneri and from erythrocytes (cae) of the fresh water carp cyprinus carpio. the purification of the isozymes was confirmed by sds acrylamide gel electrophoresis. antibodies against the purified cab and cae were then raised in rabbits. specificity was verified by immunoblotting. no cross-reaction was found between them, using the immunodot technique. cab antiserum was used to specifically localize gill ca in the tr ...19883139588
characterization of the alternative complement pathway of carp. 20113133256
isolation and partial characterization of igm-like immunoglobulins from the serum of carp (cyprinus carpio l.), frog (rana ridibunda pall.) and tortoise (testudo graeca pall.).1. igm-like immunoglobulin fractions have been isolated by combinations of precipitation procedures and gel-filtration from the serum of three vertebrate species, representative of the classes of pisces (cyprinus carpio l.), amphibia (rana ridibunda pall.) and reptilia (testudo graeca pall.). 2. the homogeneity of the isolated fractions has been confirmed electrophoretically and immunoelectrophoretically. 3. electrophoretic mobility of igm, similar for the three species studied has been observed ...19883132346
[chloromyxum barbi (myxosporea) in hypophthalmichthys molitrix (osteichthyes)].during an investigation of h. molitrix yearlings from the district of cottbus in the gdr the myxosporidian chloromyxum barbi dogel', 1934 was found in the gallbladder (not recorded before in the gdr). ch. barbi was found to be a new parasite of the silver carp.19873122603
method for the oso4-free preservation of brain tissue suitable for immunocytochemistry in tem.following conventional oso4-fixation and embedding of brain tissue in epoxide resins for ultrastructural investigations, on the one hand, the wellknown good depiction of structures is obtained, on the other hand, however, according to these procedures a considerable loss up to 80% of glycolipid-bound and up to 50% of glycoprotein-bound neuraminic acid of important native compounds has to be put up with. the fixation of brain tissue by 1% glutaraldehyde and 1% paraformaldehyde in 0.1 mol/l na-k-p ...19873122505
[determination of the number of tissue compatibility genes in the carp using the transplantation test in the third generation of induced gynogenesis].transplantations have been carried out of anal fin in carp individual progeny of the third consecutive generation of induced gynogenesis. the part of grafts accepted made 19.4 +/- 5.0% (71 transplantations have been carried out). with the data obtained, an approximate quantity of histocompatible genes has been determined in carp which amounted to 32. calculation has been done on the basis of the assumption that the part of histocompatible genes being in homozygous state in the given progeny equa ...19873121444
increased gonadotropin levels in goldfish do not result in alterations in circulating thyroid hormone levels.to determine whether increases in gonadotropin levels are capable of altering thyroid function in goldfish, plasma thyroid hormone levels were measured following induced changes in endogenous gonadotropin secretion and injection of carp gonadotropin. radio-frequency lesions placed in the nucleus preopticus periventricularis or monosodium-l-glutamate-induced lesions of the posterior nucleus lateralis tuberis (nlt) of the hypothalamus were capable of stimulating significant increases in plasma gon ...19873114043
[qualitative and quantitative distribution of 7 enzymes in organs of the rainbow trout (salmo gairdneri r.) and the carp (cyprinus carpio)]. 19873113116
physicochemical characterization of lens crystallins from the carp and biochemical comparison with other vertebrate and invertebrate crystallins.lens crystallins were isolated from the homogenate of carp (cyprinus carpio) eye lenses by gel permeation chromatography and characterized by gel electrophoresis, immunodiffusion, amino acid analysis, circular dichroism, and protein sequence analysis. three well-defined fractions corresponding to alpha/beta-, beta-, and gamma-crystallins were obtained in relative weight percentages of 26, 22, and 52%. the native molecular masses of the purified fractions were determined to be 410, 60, and 20 kda ...19873110141
ultrastructural characterization of leucocytes in the pronephros of carp (cyprinus carpio, l.).in the pronephros of carp (cyprinus carpio, l.) the following cells were found and ultrastructurally characterized: erythrocytes; lymphocytes and plasma cells; thrombocytes; neutrophilic, eosinophilic and basophilic granulocytes; phagocytic reticular cells and monocytes and non-phagocytic reticular cells. the cells could be separated on a percoll continuous density gradient and relatively pure fractions could be obtained. in vitro phagocytosis of bacteria (bacillus megaterium) was found in monoc ...19873109968
paradoxical induction of dopaminergic cells following intravitreal injection of high doses of 6-hydroxydopamine in juvenile carp retina.histofluorescence studies were conducted on two groups of juvenile carp (body length, 12.7 and 6.6 cm on the average, respectively) during certain periods (3-11 months) of retinal growth, to evaluate the destructive effect of 6-hydroxydopamine on dopaminergic and indoleamine-accumulating cells. within a certain range of doses (0.1-5.0 micrograms/eye) of the neurotoxin injected intravitreally, it was found to destroy both classes of cells in a dose-dependent manner: more cells disappeared with hi ...19873109692
immunological relationship among glycogen-protein complexes of different species.glycogen-protein complexes from the muscles of celerio euphorbiae and horse as well as from the livers of frog, carp and man were isolated and used as antigens. after immunization of rabbits antisera were obtained from which fractions of immunoglobulins were isolated. antibodies were used in agar immunodiffusion tests and in quantitative reaction of immunoprecipitation with glycogens. the immunoprecipitation test proved the occurrence of quantitative differences in cross reaction of complexes wi ...19863109350
immunosuppression of carp (cyprinus carpio l.) to ichthyophthiriasis using the corticosteroid triamcinolone acetonide.the immune response of juvenile mirror carp (cyprinus carpio l.) has been investigated in relation to protective immunity and immunosuppression to the disease ichthyophthiriasis. protective immunity was induced by exposing juvenile carp to approximately 2,000 theronts per fish for 3 hours at a concentration of approximately 80 theronts cm-3 in dechlorinated water ph 7.0-7.2, 20 +/- 2 degrees c in the dark, on three separate occasions of 14 day intervals. following each exposure, re-infection was ...19863094233
[immunoenzyme (elisa) diagnosis of spring viremia in carp].the enzymoimmunologic (elisa) method was used to diagnose spring viremia in carp. the specific antibodies to rhabdovirus carpio in the fish sera were detected by this method. reaction specificity was tested by the use of viral and control antigens, by examination of positive and negative sera and by serum-free control incubation. applying the elisa method, the antiviral antibodies can be detected after infection or vaccination: this method is therefore suitable for thorough studies and when choo ...19863094221
protein and amino acid requirements of fishes.tentative qualitative and quantitative amino acid requirements have been reported for the major species of fish reared for market or as replacement stocks for natural waters. most work has concentrated upon juvenile fish or upon rapidly growing young market fish; these have high protein dietary requirements (30-50%) that are in direct contrast to the homothermic terrestial animals. net protein utilization from the diet is similar or slightly better than that found in avian species, but energy ne ...19863089240
the effects of reserpine and lhrh or salmon gnrh analogues on gonadotropin release, ovulation and spermiation in common carp (cyprinus carpio l.).the effects of reserpine (catecholamine depletor) and lhrh analogues on gonadotropin secretion, spermiation and ovulation of common carp were investigated. injections of reserpine alone at a dose of 1 or 7 mg/kg of body weight stimulated spermiation, and reserpine at a dose of 1 mg/kg of body weight in combination with (d-arg6, trp7, leu8, pro9, net)-lhrh (s-gnrh-a) or with (d-ala6, pro9)-lhrh (lhrh-a) at a dose of 50 micrograms/kg of body weight caused an increase of plasma gonadotropin levels, ...19883072624
[acute renal failure caused by ingestion of the carp gall bladder-a report of 3 cases, with special reference to the reported cases in japan]. 19883071564
calcium action potentials in retinal cells of the carp. 19883068589
carp, a novel gene regulating the transcription of the carbamoylphosphate synthetase operon of escherichia coli.the carab operon, encoding carbamoylphosphate synthetase (cpsase; ec 6.3.5.5) is transcribed from two tandem promoters. the upstream promoter (p1) is controlled by pyrimidines and the downstream promoter (p2) is controlled by arginine. we have isolated a new type of constitutive mutation (carp) that specifically affects the control of the pyrimidine-sensitive promoter but does not appear to influence other genes of the pyrimidine pathway. the carp mutation acts in trans and is dominant, which su ...19883065518
in situ hybridization demonstrating coexpression of urotensins i, ii-alpha, and ii-gamma in the caudal neurosecretory neurons of the carp, cyprinus carpio.in order to examine the coexpression of different urotensins in the same caudal neurosecretory neurons, in situ hybridization with synthetic oligonucleotide probes specific for mrnas for urotensins i, ii-alpha, and ii-gamma (ui, uii-alpha, and uii-gamma) was applied to the caudal neurosecretory system of the carp, cyprinus carpio, together with immunohistochemistry. almost all identifiable caudal neurosecretory neurons were labeled with the oligonucleotide and cdna probes for ui mrna. further, a ...19883056775
effect of teleost gnrh on reinitiation of oocyte meiosis in goldfish, in vitro.the effects of native and an agonist analogue of teleost gonadotropin-releasing hormone (gnrh) [(d-arg6, trp7, leu8, pro9-nhet)-gnrh; tgnrh-a] on the hormone-induced meiotic maturation of goldfish oocytes were investigated. incubation of follicle-enclosed goldfish oocytes with either carp gonadotropin (gth) or meiogenic progestogens, including 17 alpha-hydroxyprogesterone (hp) and 17 alpha,20 beta-dihydroxyprogesterone (dhp), reinitiated oocyte meiosis in vitro, as indicated by germinal vesicle ...19883044146
regulation of dopamine release from interplexiform cell processes in the outer plexiform layer of the carp retina.the gamma-aminobutyric acid (gaba) antagonists bicuculline and picrotoxin stimulate a four- to fivefold increase in endogenous dopamine release from isolated intact carp retina. the release evoked by these agents is ca2+ dependent, a finding suggesting a vesicular release. using light microscopic autoradiography, we have localized the sites of dopamine release to the dopaminergic interplexiform cell processes of the outer plexiform layer, which synapse onto horizontal cells. our findings support ...19873039059
dielectric, haematological and biochemical studies of detergent toxicity in fish blood.blood characteristics in dielectric, haematological and biochemical terms of the fish cyprinus carpio exposed to a sublethal concentration of sodium alkyl benzene sulphonate were compared with those from untreated control fish; recovery from the test solution was also checked. trends of change in the majority of chosen parameters of blood with time of fish exposure to anionic detergent were significantly linear. a decline was noted in erythrocyte count, haematocrit, blood haemoglobin concentrati ...19873035591
induction of xanthophores from non-pigmented dermal cells of xanthic goldfish in vitro.to identify precursor cells of xanthophores (xanthoblasts), non-pigmented cells without any phenotypic traits as pigment cells were isolated from the dermal tissue of xanthic goldfish with an adult color pattern and cultured in a medium containing 1 mm db-camp or 0.25 u/ml acth and 10% carp serum. these non-pigmented cells differentiated into xanthophores which showed a dendritic morphology and contained a large quantity of fluorescent pteridines and numerous vesicular inclusions. sepiapterin wa ...20103032461
hormonal regulation of hepatic glycogenolysis in the carp, cyprinus carpio.carp (cyprinus carpio) liver maintained normal glycogen content and enzyme complement for several days in organ culture. epinephrine-stimulated glycogenolysis, phosphorylase activation, and cyclic amp (camp) accumulation in a concentration-dependent manner with ec50s of 100, 100, and 500 nm, respectively. these actions were blocked by the beta-adrenergic antagonist, propranolol, but not by the alpha-adrenergic antagonist phentolamine. glycogenolysis and tissue camp were uninfluenced by 10(-6) m ...20103032003
acid-base regulation and ion transfers in the carp (cyprinus carpio): ph compensation during graded long- and short-term environmental hypercapnia, and the effect of bicarbonate infusion.to study both temporal and quantitative effects of hypercapnia on the extent of ph compensation in the arterial blood, specimens of carp (cyprinus carpio) were exposed to a pco2 of about 7.5 mmhg (1 mmhg = 133.3 pa) (1% co2) in the environmental water for several weeks, and a second group of animals was subjected to an environmental pco2 of about 37 mmhg (5% co2) for up to 96 h. a third series of experiments was designed to test the possibility that infusion of bicarbonate would increase the ext ...19863027233
circulating forms of alpha msh in the carp and trout blood: an hplc and ria study.the three forms of msh(desacetyl-, monoacetyl-, and diacetyl) circulate in the carp and trout blood. their proportions there are as follows: in the carp, the desacetylated form is 2.5% of total msh and the monoacetylated form 72% of the acetylated forms of alpha msh. in the trout, similar proportions were found: desacetyl alpha msh was 5% of total alpha msh and monoacetyl-, 70% of the acetylated mshs.19863023175
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