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damage potential of grasshoppers (orthoptera: acrididae) on early growth stages of small-grains and canola under subarctic conditions.we characterized the type and extent of grasshopper injury to above- and below-ground plant parts for four crops [barley (hordeum vulgare l.), oats (avena sativa l.), wheat (triticum aestivum l.), and canola (brassica campestris l.)] commonly grown, or with potential to grow, in central alaska. cages were placed on 48 pots containing plants in second to third leaf stages and stocked with 0, 2, 4, and 6 first-instar melanoplus sanguinipes f. pot(-1). plants were harvested 22 d after planting. ste ...200314503591
activation parameters of the blue shift (shibata shift) subsequent to protochlorophyllide phototransformation.the shibata shift was analyzed in flash irradiated wheat (triticum aestivum, l., cult. mv17) leaf homogenates in the pressure range of 0.1 to 500 mpa, at temperatures of 20, 30 and 40 degrees c. the kinetics of the blue shift (called shibata shift in case of intact leaves) was followed by repeated recording of fluorescence emission spectra after phototransformation. at 20 degrees c, above 100 mpa, the blue shift slowed down remarkably. two components of the blue shift could be distinguished, one ...200314499597
[respiratory inhibition of roots of triticum vulgare with sodium diethyldithiocarbamate (ddc) and induced cytoplasmic modifications in the meristematic cells]. 196414250626
a sequential growth response to gibberellic acid, kinetin and indolyl-3-acetic acid in the wheat coleoptile (triticum vulgare l.). 196113786824
[biological effects of substances inhibiting monoamine oxidase. i. action on the development of "triticum vulgare"]. 196013772440
[variations in the distribution of ribonucleoproteins in the tips of wheat (triticum vulgare vill.) sown in winter, in spring, and in autumn]. 196013737579
[influence of some bacteriostatic agents on the full growth cycle of triticum vulgare]. 201613706897
hmw and lmw glutenin alleles among putative tetraploid and hexaploid european spelt wheat (triticum spelta l.) progenitors.the allelic compositions of high- and low-molecular-weight subunits of glutenins (hmw-gs and lmw-gs) among european spelt ( triticum spelta l.) and related hexaploid and tetraploid triticum species were investigated by one- and two-dimensional polyacrylamide-gel electrophoresis (page) and capillary electrophoresis (ce). a total of seven novel glutenin alleles (designated a1a*, b1d*, b1g*, b1f*, b1j*, d1a* at glu-1 and a3h at the glu-3 loci, respectively) in european spelt wheat were detected by ...200313679994
identification and mapping of genetic loci affecting the free-threshing habit and spike compactness in wheat ( triticum aestivum l.).recombinant inbred lines of the international triticeae mapping initiative (itmi) mapping population were used to localize genetic loci that affect traits related to the free-threshing habit (percent threshability, glume tenacity, and spike fragility) and to spike morphology (spike length, spikelet number, and spike compactness) of wheat ( triticum aestivum l.). the itmi population was planted in three environments during 1999 and 2000, and phenotypic and genotypic data were used for composite i ...200413679977
[effects of various steroids of corticotropin (acth) on the processes of germination & growth of seeds of triticum vulgare & phaseolus vulgaris]. 200513591741
[influence of some bacteriostatics on the first stage of development of wheat, triticum vulgare]. 200613537975
additional genes in triticum vulgare for resistance to erysiphe graminis tritici. 195413229838
the biosynthesis and metabolism of betaines in plants. iii. studies on the biosynthesis of precursors of glycinebetaine in seedlings of wheat (triticum vulgare vill.). 195413208596
the resistance of twelve varieties of triticum vulgare to erysiphe graminis tritici. 195313093525
[apex of triticum vulgare; modalities of resumption of mitoses at germination and its vegetative function]. 195313067293
[new finding of inertia of the so-called initial axials in the root meristema of triticum vulgare]. 195313059960
[research on anaerobiosis of embryos of triticum vulgare]. 195212978052
cis-jasmone treatment induces resistance in wheat plants against the grain aphid, sitobion avenae (fabricius) (homoptera: aphididae).cis-jasmone is a plant volatile known to have roles as an insect semiochemical and in inducing plant defence. it was evaluated in laboratory and field trials for control of cereal aphids. in an olfactometer bioassay cis-jasmone was repellent to alatae of the grain aphid, sitobion avenae (fabricius) (homoptera: aphididae). moreover, wheat, triticum aestivum (l), seedlings sprayed with formulated cis-jasmone 24 h previously were less susceptible to attack by s. avenae than control plants. in field ...200312974355
fatty acid biosynthesis in mitochondria of grasses: malonyl-coenzyme a is generated by a mitochondrial-localized acetyl-coenzyme a carboxylase.we present biochemical evidence for the occurrence of a 250-kd multifunctional acetyl-coenzyme a carboxylase in barley (hordeum vulgare) mitochondria. organelles from 6-d-old barley seedlings were purified by differential centrifugation and percoll density gradient centrifugation. upon analysis by two-dimensional blue-native (bn)/sds-page, an abundant 250-kd protein can be visualized, which runs at 500 kd on the native gel dimension. a similar 500-kd complex is present in etioplasts from barley. ...200312972648
electrical potentials of plant cell walls in response to the ionic environment.electrical potentials in cell walls (psi(wall)) and at plasma membrane surfaces (psi(pm)) are determinants of ion activities in these phases. the psi(pm) plays a demonstrated role in ion uptake and intoxication, but a comprehensive electrostatic theory of plant-ion interactions will require further understanding of psi(wall). psi(wall) from potato (solanum tuberosum) tubers and wheat (triticum aestivum) roots was monitored in response to ionic changes by placing glass microelectrodes against cel ...200312970506
overexpression of a gene encoding hydrogen peroxide-generating oxalate oxidase evokes defense responses in sunflower.oxalate oxidase (oxo) converts oxalic acid (oa) and o(2) to co(2) and hydrogen peroxide (h(2)o(2)), and acts as a source of h(2)o(2) in certain plant-pathogen interactions. to determine if the h(2)o(2) produced by oxo can function as a messenger for activation of defense genes and if oxo can confer resistance against an oa-producing pathogen, we analyzed transgenic sunflower (helianthus annuus cv smf3) plants constitutively expressing a wheat (triticum aestivum) oxo gene. the transgenic leaf tis ...200312970484
effect of air pollution on peri-urban agriculture: a case study.peri-urban agriculture is vital for the urban populations of many developing countries. increases in both industrialization and urbanization, and associated air pollution threaten urban food production and its quality. six hour mean concentrations were monitored for so(2), no(2) and o(3) and plant responses were measured in terms of physiological characteristics, pigment, biomass and yield. parameter reductions in mung bean (vigna radiata), palak (beta vulgaris), wheat (triticum aestivum) and mu ...200312963293
hormonal regulation of the onset of endosperm development in amphimictic triticum aestivum l. and apomictic taraxacum officinale web. species. 201712959073
isolation and identification of actinobacteria from surface-sterilized wheat roots.this is the first report of filamentous actinobacteria isolated from surface-sterilized root tissues of healthy wheat plants (triticum aestivum l.). wheat roots from a range of sites across south australia were used as the source material for the isolation of the endophytic actinobacteria. roots were surface-sterilized by using ethanol and sodium hypochlorite prior to the isolation of the actinobacteria. forty-nine of these isolates were identified by using 16s ribosomal dna (rdna) sequencing an ...200312957950
cloning, characterization and regulation of a family of phi class glutathione transferases from wheat.six phi (f) class glutathione transferases (gsts) were cloned from bread wheat (triticum aestivum l.) treated with the herbicide safener fenchlorazole ethyl and named tagstf1-6. recombinant tagstfs were assayed for glutathione conjugating activity towards xenobiotics including herbicides and for glutathione peroxidase (gpox) activity. tagstf1, which resembled zmgstf1, the dominant gst in maize (zea mays), was highly active in conjugating 1-chloro-2,4-dinitrobenezene (cdnb) but had low activities ...200312956529
enhanced pesticide sorption by soils containing particulate matter from crop residue burns.lack of proper techniques to isolate black carbon (bc) from soils has hindered the understanding of their roles in the sorption and environmental fate of organic contaminants in soils and sediments. the burning of crop residues may be the primary source of bc in agricultural soils. in this study, wheat (triticum aestivum l.) and rice (oryza sativa l.) residues were burned, and the resulting particulate matter (ashes) along with a soil were used to sorb diuron from water. calculations indicated t ...200312953876
genetic transformation of indian bread (t. aestivum) and pasta (t. durum) wheat by particle bombardment of mature embryo-derived calli.particle bombardment has been successfully employed for obtaining transgenics in cereals in general and wheat in particular. most of these procedures employ immature embryos which are not available throughout the year. the present investigation utilizes mature seeds as the starting material and the calli raised from the hexaploid triticum aestivum and tetraploid triticum durum display a high regeneration response and were therefore used as the target tissue for genetic transformation by the biol ...200312952555
fluorescence lifetimes of protochlorophyllide in plants with different proportions of short-wavelength and long-wavelength protochlorophyllide spectral forms.dark-grown leaves of maize (zea mays), wheat (triticum aestivum), wild-type pea (pisum sativum) and its light-independent photomorphogenesis mutant (lip1) have different proportions of protochlorophyllide (pchlide) forms as revealed by low-temperature fluorescence emission spectra. four discrete spectral forms of pchlide, with emission peaks around 633, 640, 656 and 670 nm, could be distinguished after gaussian deconvolution. in maize and wheat the 656 nm component was the most prominent, wherea ...200312945590
[detection of the introgression of genome elements of aegilops cylindrica host. into triticum aestivum l. genome with issr-analysis].comparative analysis of introgressive and parental forms of wheat was carried out to reveal the sites of donor genome with new loci of resistance to fungal diseases. by issr-method 124 issr-loci were detected in the genomes of 18 individual plants of introgressive line 5/20-91; 17 of them have been related to introgressive fragments of ae. cylindrica genome in t. aestivum. it was shown that issr-method is effective for detection of the variability caused by introgression of alien genetic materia ...200812945176
[genealogy-based comparative analysis of loose smut resistance of spring common wheat cultivars].comparative genealogical analysis was conducted for loose smut-resistant and susceptible common wheat cultivars of three regions: russia, canada, and india. pedigree analysis of differentiator varieties revealed several sources of the ut1, ut3, and ut4 genes. tracking resistance transmission in extended pedigrees allowed identification of resistance donors, sources, and, in some cases, putative genes in russian, canadian, and indian cultivars. a contingency table was constructed with the data on ...200312942780
field response of wheat to arbuscular mycorrhizal fungi and drought stress.mycorrhizal plants often have greater tolerance to drought than nonmycorrhizal plants. this study was conducted to determine the effects of arbuscular mycorrhizal (am) fungi inoculation on growth, grain yield and mineral acquisition of two winter wheat (triticum aestivum l.) cultivars grown in the field under well-watered and water-stressed conditions. wheat seeds were planted in furrows after treatment with or without the am fungi glomus mosseae or g. etunicatum. roots were sampled at four grow ...200412942358
growth responses of wheat (triticum aestivum l. var. hd 2329) exposed to ambient air pollution under varying fertility regimes.the problem of urban air pollution has attracted special attention in india due to a tremendous increase in the urban population; motor vehicles vis a vis the extent of energy utilization. field studies were conducted on wheat crops (triticum aestivum l. var. hd 2329) by keeping the pot-grown plants in similar edaphic conditions at nine different sites in allahabad city to quantify the effects of ambient air pollution levels on selected growth and yield parameters. air quality monitoring was don ...200312941977
estimating nitrate leaching with a transfer function model incorporating net mineralization and uptake of nitrogen.because of the complex interaction of chemical and biological processes of nitrogen (n) in soils, it is difficult to estimate the leaching of nitrate with various n transformations in porous media. in this study, a transfer function model was developed to simulate the outflow concentration of nitrate in soils during the growth of winter wheat (triticum aestivum l.), taking into account the main n transformations using source and sink terms. the source and sink terms were treated as inputs to the ...201312931902
comparison of triticonazole dissipation after seed or soil treatment.this study examined the long-term fate of the fungicide triticonazole (ttz; 5-[(4-chlorophenyl)methylene]-2,2-dimethyl-1-(1h-1,2,4-triazol-1-ylmethyl)cydopentanol) applied at a normal field dose (8.9 g ha(-1)) via seed treatment, which is the normal alternative in practice. the ttz was applied to wheat (triticum aestivum l.) grains as a disinfectant before sowing or spraying on bare soil for comparison and reference to the seed treatment. the seeds were germinated and grown in pots in a greenhou ...200612931880
phenotypic assessment and mapped markers for h31, a new wheat gene conferring resistance to hessian fly (diptera: cecidomyiidae).a new source of resistance to the highly virulent and widespread biotype l of the hessian fly, mayetiola destructor (say), was identified in an accession of tetraploid durum wheat, triticum turgidum desf., and was introgressed into hexaploid common wheat, triticum aestivum l. genetic analysis and deletion mapping revealed that the common wheat line contained a single locus for resistance, h31, residing at the terminus of chromosome 5bs. h31 is the first hessian fly-resistance gene to be placed o ...200312928782
genetic analysis, molecular tagging and mapping of the thermo-sensitive genic male-sterile gene (wtms1) in wheat.a thermo-sensitive genic male-sterile (tgms) wheat line ( triticum aestivum l.) bny-s was obtained from the spontaneous mutant of bny-f. its fertility was decided by the temperature during the differentiation stage of the spikelets. bny-s was completely sterile when the temperature was lower than 10 degrees c during the differentiation stage of the spikelets, but fertile when the temperature was higher than 10 degrees c. genetic analysis indicated that the sterility of bny-s was controlled by a ...200312928780
detection of qtls for stagonospora glume blotch resistance in swiss winter wheat.stagonospora nodorum is the causal agent of the stagonospora glume blotch disease in hexaploid wheat. the swiss winter bread wheat cv. 'arina' has a highly effective, durable and quantitative glume blotch resistance. we studied 240 single seed descent (ssd)-derived lines of an 'arina x forno' f(5:7) population to identify and map quantitative trait loci (qtls) for glume blotch resistance under natural infestation. using composite interval mapping (cim) and lod>4.5, we detected two chromosomal re ...200312928778
[studies on application of apage in male sterile wheat with alloplamic cytoplasm].four male sterile wheat with aeglops kotschyi, ae. variabilis, ae. ventricosa and ae. bicornis cytoplasm, were crossed with some wheat materials, and from the crosses a series of maintenance lines were selected. the apage(acid polyacrylamide gel electrophoresis) analysis indicated that most maintenance lines possessed the gliadin marker gld1b3 of 1bl/1rs translocation chromosome. the root tip chromosomes of this maintenance lines had two satellite chromosomes, but other maintenance lines without ...200312924162
[effects of combined pollution of heavy metals on root vitality of wheat seeds].the combined effects of cu, cd, pb and zn on the growth and activity of the seminal roots of wheat (triticum aestivum) were studied by the method of quadratic regression revolution design. the mathematical models describing the above relations were established. as for the toxicity to the root vitality, their single effects, marginal utility and combined effects between two heavy metals were analyzed. the results showed that pb had the most important effect on the activity of root, and then, cu, ...200312920906
contact activity of difenzoquat differs from that of paraquat.difenzoquat herbicide is used for post-emergence control of wild oat (avena fatua l) in small grain crops. its mechanism of action is not known, but appears to have both paraquat-like contact activity and systemic growth-inhibition activity. experiments were conducted in vitro to compare the contact activity of difenzoquat and paraquat, to examine the contact activity of difenzoquat in difenzoquat-resistant (accession lcs) and -susceptible (accession sb 18) a fatua biotypes and -tolerant 'ernest ...200312916774
thylakoid-bound ascorbate peroxidase mutant exhibits impaired electron transport and photosynthetic activity.in chloroplasts, stromal and thylakoid-bound ascorbate peroxidases (tapx) play a major role in the removal of h(2)o(2) produced during photosynthesis. here, we report that hexaploid wheat (triticum aestivum) expresses three homeologous tapx genes (taapx-6a, taapx-6b, and taapx-6d) mapping on group-6 chromosomes. the tapx activity of a mutant line lacking taapx-6b was 40% lower than that of the wild type. when grown at high-light intensity photosystem ii electron transfer, photosynthetic activity ...200312913166
tavrt-1, a putative transcription factor associated with vegetative to reproductive transition in cereals.the molecular genetics of vernalization, defined as the promotion of flowering by cold treatment, is still poorly understood in cereals. to better understand this mechanism, we cloned and characterized a gene that we named tavrt-1 (wheat [triticum aestivum] vegetative to reproductive transition-1). molecular and sequence analyses indicated that this gene encodes a protein homologous to the mads-box family of transcription factors that comprises certain flowering control proteins in arabidopsis. ...012913142
cold-specific and light-stimulated expression of a wheat (triticum aestivum l.) cor gene wcor15 encoding a chloroplast-targeted protein.wcor15, a member of the wheat cold-responsive (cor) gene family, has been isolated and characterized. the deduced polypeptide wcor15 (mw=14.7 kda) showed high homology to the previously identified wheat and barley cor proteins. southern blot analysis using diploid, tetraploid and hexaploid wheat and diploid aegilops species showed that the wheat and related wild genomes possessed multiple copies of wcor15 homologues. five copies were assigned to the homologous group 2 chromosomes by nulli-tetras ...200312909691
quantitative trait loci and molecular markers associated with wheat allelopathy.wheat ( triticum aestivum l.) has been examined for allelopathic potential against annual ryegrass ( lolium rigidum). the bioassay technique, 'equal-compartment-agar-method', was employed to evaluate seedling allelopathy in a doubled-haploid (dh) population derived from cv sunco (weakly allelopathic) and cv tasman (strongly allelopathic). a significant difference in allelopathic activity was found among the dh lines, which inhibited the root length of ryegrass across a range from 23.7 to 88.3%. ...200312904863
pesticide adsorptivity of aged particulate matter arising from crop residue burns.particulates (ashes) arising from the burning of crop residues are potentially effective adsorbents for pesticides in agricultural soils. to determine the long-term adsorptive sustainability of ashes, a wheat (triticum aestivum l.) ash was aged under environmentally relevant conditions (in cacl(2) solution at room temperature and ph 7) in soil extract for 1 month and in a soil (1% ash) for a period of up to 12 months. the aged ash and ash-amended soil were used to sorb diuron from water. the diu ...200312903968
presence of yeasts in southern italian sourdoughs from triticum aestivum flour.samples of sourdoughs obtained from 13 artisanal bakeries located in the molise and campania regions were analysed. the sourdoughs were produced with the exclusive use of triticum aestivum wheat flour. ph values of sourdoughs from molise were generally lower than those from campania. the number of yeasts in the samples of sourdoughs from molise was generally higher than in those from campania, which in two cases evidenced counts about 2 log cfu x g(-1). by utilising and comparing traditional and ...200312900033
an integrative genetic linkage map of winter wheat (triticum aestivum l.).we constructed a genetic linkage map based on a cross between two swiss winter wheat ( triticum aestivum l.) varieties, arina and forno. two-hundred and forty f(5) single-seed descent (ssd)-derived lines were analysed with 112 restriction fragment length polymorphism (rflp) anonymous probes, 18 wheat cdna clones coding for putative stress or defence-related proteins and 179 simple-sequence repeat (ssr) primer-pairs. the 309 markers revealed 396 segregating loci. linkage analysis defined 27 linka ...200312898031
chromosomal location of a race-specific resistance gene to mycosphaerella graminicola in the spring wheat st6.septoria tritici blotch, caused by mycosphaerella graminicola, is a serious foliar disease of wheat worldwide. qualitative, race-specific resistance sources have been identified and utilized for resistant cultivar development. however, septoria tritici blotch resistant varieties have succumbed to changes in virulence of m. graminicola on at least three continents. the use of resistance gene pyramids may slow or prevent the breakdown of resistance. a clear understanding of the genetics of resista ...200312898022
molecular mapping of novel genes controlling fusarium head blight resistance and deoxynivalenol accumulation in spring wheat.fusarium head blight of wheat is an extremely damaging disease, causing severe losses in seed yield and quality. the objective of the current study was to examine and characterize alternate sources of resistance to fusarium head blight (fhb). ninety-one f1-derived doubled haploid lines from the cross triticum aestivum 'wuhan-1' x triticum aestivum 'maringa' were examined for disease reaction to fusarium graminearum by single-floret injection in replicated greenhouse trials and by spray inoculati ...200312897863
qtl mapping of partial resistance in winter wheat to stagonospora nodorum blotch.stagonospora nodorum blotch is an important foliar and glume disease in cereals. inheritance of resistance in wheat appears to be quantitative. to date, breeding of partially resistant cultivars has been the only effective way to combat this pathogen. the partial resistance components, namely length of incubation period, disease severity, and length of latent period, were evaluated on a population of doubled haploids derived from a cross between the partially resistant triticum aestivum 'liwilla ...200312897862
mature green waste compost enhances growth and nitrogen uptake in wheat (triticum aestivum l.) and oilseed rape (brassica napus l.) through the action of water-extractable factors.a series of field and pot trials were carried out to determine the effects of growing wheat and oilseed rape in soils supplemented with green waste composts and provided with additional fertilisers. it was shown consistently that the response of wheat and rape to compost and fertiliser applied together was greater than the responses to the individual additives, but only when very stable compost was used (>10 months processing). experiments with 15n-labelled fertiliser showed that wheat was able ...200312895554
soil properties and crop productivity as influenced by flyash incorporation in soil.field experiments were carried out during 1996-97 at gulawathi, muthiani and salarpur villages, iari farm, new delhi and ncpp campus, dadri to evaluate changes in soil characteristics and growth of wheat (triticum aestivum l.), mustard (brassica juncea l.), lentil (lence esculenta moench.), rice (oryza sativa l.) and maize (zea mays l.) by varying amounts of flyash addition (up to 50t ha(-1)) in soils at sowing/transplanting time of crops. flyash addition in areas adjoining ncpp thermal power pl ...200312889638
[rapd-based analysis of introgression of barley genetic material into the genome of alloplasmic wheat lines (hordeum geniculatum all./ triticum aestivum l.)].genomes of three alloplasmic wheat lines obtained on the basis of barley--wheat hybrid horderum geniculatum all. (2n = 28) x triticum aestivum l. (2n = 42)(pyrotrix 28) were examined using random amplified polymorphic dna (rapd) analysis. line l-29 was obtained after first backcross of the initial hybrid with the wheat variety pyrotrix 28 and ten subsequent self-pollinating generations. this line was represented by euploid plants with typical to the common wheat chromosome number (2n = 42), as w ...200312884518
growth promotion and an increase in cell wall extensibility by silicon in rice and some other poaceae seedlings.the effect of silicon on organ growth and its mechanisms of action were studied in rice ( oryza sativa l. cv. koshihikari), oat ( avena sativa l. cv. victory), and wheat ( triticum aestivum l. cv. daichino-minori) seedlings grown in the dark. applying silicon in the form of silicic acid to these seedlings via culture solution resulted in growth promotion of third (rice) or second (oat and wheat) leaves. the optimal concentration of silicon was 5-10 mm. no growth promotion was observed in early o ...200212884045
modeling and control for closed environment plant production systems.a computer program was developed to study multiple crop production and control in controlled environment plant production systems. the program simulates crop growth and development under nominal and off-nominal environments. time-series crop models for wheat (triticum aestivum), soybean (glycine max), and white potato (solanum tuberosum) are integrated with a model-based predictive controller. the controller evaluates and compensates for effects of environmental disturbances on crop production s ...200212882224
is microtubule disassembly a trigger for cold acclimation?cold acclimation was followed in three cultivars of winter wheat (triticum aestivum l.) that differ in freezing tolerance, using root growth as the indicator. during acclimation (followed through 7 d at 4 degrees c), growth rate progressively recovered. the recovery was fast in the tolerant, slow in the sensitive cultivars. the development of freezing tolerance was followed by a challenging cold shock administered after various time intervals of acclimation. acclimation proceeded faster in the t ...200312881495
time and cell cycle dependent formation of heterogeneous tubulin arrays induced by colchicine in triticum aestivum root meristem.we have investigated the appearance and reorganization of tubulin-containing arrays induced by colchicine in the root meristem of wheat triticum aestivum, using immunostaining and electron microscopy. colchicine caused depolymerization of microtubules and formation of tubulin cortical strands composed of filamentous material only in c-mitotic cells. after prolonged exposure to the drug, both interphase and c-mitotic cells acquired needle-type bundles, arranged as different crystalloids and/or ma ...200312867155
unraveling thioredoxin-linked metabolic processes of cereal starchy endosperm using proteomics.application of a thiol-specific probe, monobromobimane, with proteomics and enzyme assays led to the identification of 23 thioredoxin targets in the starchy endosperm of mature wheat seeds (triticum aestivum cv. butte), almost all containing at least two conserved cysteines. the identified targets, 12 not known to be thioredoxin-linked, function in a spectrum of processes: metabolism (12 targets), protein storage (three), oxidative stress (three), protein degradation (two), protein assembly/fold ...200312860404
an isoleucine residue within the carboxyl-transferase domain of multidomain acetyl-coenzyme a carboxylase is a major determinant of sensitivity to aryloxyphenoxypropionate but not to cyclohexanedione inhibitors.a 3,300-bp dna fragment encoding the carboxyl-transferase domain of the multidomain, chloroplastic acetyl-coenzyme a carboxylase (accase) was sequenced in aryloxyphenoxypropionate (app)-resistant and -sensitive alopecurus myosuroides (huds.). no resistant plant contained an ile-1,781-leu substitution, previously shown to confer resistance to apps and cyclohexanediones (chds). instead, an ile-2,041-asn substitution was found in resistant plants. phylogenetic analysis of the sequences revealed tha ...200312857850
calmodulin is involved in heat shock signal transduction in wheat.the involvement of calcium and calcium-activated calmodulin (ca(2+)-cam) in heat shock (hs) signal transduction in wheat (triticum aestivum) was investigated. using fluo-3/acetoxymethyl esters and laser scanning confocal microscopy, it was found that the increase of intracellular free calcium ion concentration started within 1 min after a 37 degrees c hs. the levels of cam mrna and protein increased during hs at 37 degrees c in the presence of ca(2+). the expression of hsp26 and hsp70 genes was ...200312857801
allopolyploidy alters gene expression in the highly stable hexaploid wheat.hexaploid wheat (triticum aestivum) contains triplicated genomes derived from three distinct species. to better understand how different genomes are coordinated in the same nucleus of the hexaploid wheat, we globally compared gene expression of a synthetic hexaploid wheat with its diploid (aegilops tauschii) and tetraploid (t. turgidum) parents by cdna-aflp display. the results suggested that the expression of a significant fraction of genes was altered in the synthetic hexaploid; most appeared ...200312856945
categories of resistance at four growth stages in three wheats resistant to the russian wheat aphid (homoptera: aphididae).laboratory experiments were conducted to determine categories of resistance to russian wheat aphid, diuraphis noxia (mordvilko), in three wheats, triticum aestivum l, (pi 372129, pi 243781, and pi 222668) at zadoks growth stages 10, 20, 30, and 40. 'tam 107' was used as the susceptible standard. antixenosis was observed in pi 222668 and pi 372129. antibiosis was expressed as reduced nymphipositional period, daily nymph production, and fecundity at the jointing (zadoks 30) and boot (zadoks 40) st ...200312852604
dhplc scoring of a snp between promoter sequences of hmw glutenin x-type alleles at the glu-d1 locus in wheat.the promoter regions of hmw glutenin x-type genes at the glu-d1 locus were surveyed for snps within a subpopulation of german bread wheat cultivars. on the basis of the promoter sequences of hmw glutenin subunit genes glu-a1-x1, glu-a1-x2, glu-b1-x1, glu-b1-x7, glu-d1-x2, and glu-d1-x5, an amplification refractory mutation system assay was designed to selectively amplify dx-specific pcr fragments. comparative sequence analysis among seven glu-d1-x2 and seven glu-d1-x5 wheat cultivars only confir ...200312848495
asymmetric somatic hybridization between wheat (triticum aestivum l.) and agropyron elongatum (host) nevishi.suspension-derived protoplasts of agropyron elongatum irradiated by ultra-violet light (uv) were fused with the suspension-derived protoplasts of triticum astivum using peg. fertile intergeneric somatic hybrid plants were produced and various hybrid lines have been selected and propagated in successive generations. their hybrid nature was confirmed by analysis of profiles of isozymes, rapds, and 5s rdna spacer sequences, and via gish analysis. by the procedure described, the phenotype and chromo ...200312845444
accumulation of hydroxycinnamic acid amides in winter wheat under snow.it was found that the content of antifungal compounds p-coumaroylagmatine [1-(trans-4'-hydroxycinnamoylamino)-4-guanidinobutane] and p-coumaroyl-3-hydroxyagmatine [1-(trans-4'-hydroxycinnamoylamino)-3-hydroxy-4-guanidinobutane] in the crown of winter wheat (triticum aestivum l. cv chihokukomugi) significantly increased under snow cover. this finding suggests that the accumulation of these hydroxycinnamic acid amides was caused by winter stress and related to protecting the plant against snow mol ...200312843649
genetics of growth habit (spring vs winter) in common wheat: confirmation of the existence of dominant gene vrn4.the number of dominant vrn genes in common wheat, triticum aestivum l., is estimated. data were obtained supporting pugsley's and gotoh's data on the presence of a dominant gene vrn4 in near-isogenic line 'triple dirk f'. the presence of a dominant gene vrn4 in line 'gabo-2' of cultivar 'gabo', which was used by pugsley as a donor of the gene vrn4 for the near-isogenic line 'triple dirk f', was also confirmed. the vrn2 and vrn4 relationship and their chromosomal location are discussed. it was de ...200312838388
[effects of co2 enrichment, nitrogen and water on photosynthesis, evapotranspiration and water use efficiency of spring wheat].spring wheat (triticum aestivum l.) was treated with 2 atmospheric co2 concentrations (350 and 700 mumol.mol-1), 2 levels of soil moisture (wet and drought), and 5 levels of nitrogen fertilizer (0, 50, 100, 150, 200 mg.kg-1 soil). the results showed that elevated atmospheric co2 increased photosynthetic rate more in drought than in wet treatment. photosynthetic rate increased with the increase of nitrogen fertilizer. stomatal conductance decreased with co2 enrichment, which was more in drought t ...200312836547
effect of zearalenone treatment on the production of wheat haploids via the maize pollination system.the ability of zearalenone (zen) to stimulate the growth of haploid wheat embryos formed following the pollination of wheat spikes with maize pollen was tested. the maize pollination system was used as a model to compare the activity of zen with that of auxin analogues. three solutions, each with a different concentration of zen (6.0, 3.0 or 1.5 microm), and a solution of 2,4-dichlorophenoxyacetic acid (control) were tested for their effect on ovary swelling, frequency of embryo formation and th ...200312835995
agrobacterium-mediated large-scale transformation of wheat (triticum aestivum l.) using glyphosate selection.an agrobacterium-mediated transformation system with glyphosate selection has been developed for the large-scale production of transgenic plants. the system uses 4-day precultured immature embryos as explants. a total of 30 vectors containing the 5-enol-pyruvylshikimate-3-phosphate synthase gene from agrobacterium strain cp4 (aroa:cp4), which confers resistance to glyphosate, were introduced into wheat using this system. the aroa:cp4 gene served two roles in this study-selectable marker and gene ...200312835912
characterization of six wheat x thinopyrum intermedium derivatives by gish, rflp, and multicolor gish.restriction fragment length polymorphism (rflp) analysis and multicolor genomic in situ hybridization (gish) are useful tools to precisely characterize genetic stocks derived from crosses of wheat (triticum aestivum) with thinopyrum intermedium and thinopyrum elongatum. the wheat x th. intermedium derived stocks designated z1, z2, z3, z4, z5, and z6 were initially screened by multicolor gish using aegilops speltoides genomic dna for blocking and various combinations of genomic dna from th. inter ...200312834067
[effects of amino acid-n and ammonium-n on wheat seedlings under sterile culture].the dry weight, total n, and glutamate-oxaloacetate transaminase(got) and glutamate-pyruvate transaminase (gpt) activities in roots and leaves of wheat seedlings (triticum aestivum) grown with ammonium sulfate or amino acids (glycine, glutamate or lysine) were studied under sterile sand culture. the results showed that both nh4(+)-n and amino acid-n could be absorbed by wheat. the total n of plant fed with nh4(+)-n was similar to that fed with amino acid-n. the dry weight of plants grown 30 days ...200312827867
tissue-specific expression and localization of safener-induced glutathione s-transferase proteins in triticum tauschii.glutathione s-transferase (gst; ec 2.5.1.18) gene expression was examined in the coleoptile and new leaf tissue of etiolated shoots of the diploid wheat species triticum tauschii (coss.) schmal., which is considered to be a progenitor and the d-genome donor to cultivated, hexaploid bread wheat triticum aestivum l. gst expression (mrna, protein, and enzyme activity with a herbicide substrate) in these shoot tissues was examined in response to herbicide safener treatment. two different antibody pr ...200312827355
glyphosate applied preharvest induces shikimic acid accumulation in hard red spring wheat (triticum aestivum).glyphosate is a nonselective herbicide used as a harvest aid in a variety of crops. glyphosate is absorbed into the foliage and translocated to metabolically active regions in the plant where it interferes with the shikimic acid pathway. experiments were conducted to determine the accumulation and distribution of shikimic acid in wheat treated with glyphosate at soft and hard dough stages of kernel development and to determine the fate of shikimic acid during milling and bread making. elevated l ...200312822937
[differential expression of genes related to photoperiod-temperature sensitive genic male sterility in wheat, revealed by mrna differential display using g-box family primer].mrna differential display with g-box family primer was used to analyze the differential expression of genes of the photoperiod-temperature sensitive genic male sterile(ptsgms) line of wheat, bau3338, between the sterile and fertile conditions. the result indicated that gene expression was significantly different between the two types of condition during the fertility transformation phase. the twelve qualitatively different dna bands were identified with reverse northern blot hybridization and fi ...200312812077
[development and application of a genome specific pcr marker for haynaldia villosa].random amplified polymorphic dna (rapd) analysis was performed on common wheat chinese spring, h. villosa, addition lines of h. villosa chromosome in cs, substitution line 3v of h. villosa chromosome in triticum aestivum. a genome specific polymorphic dna segment from h. villosa, opf02757, was obtained. on the basis of cloning and sequencing of opf02757, two pcr primers were designed and a genome specific pcr marker for h. villosa was established. the pcr marker including 677 bp was localized on ...200312812061
a centromeric tandem repeat family originating from a part of ty3/gypsy-retroelement in wheat and its relatives.from a wild diploid species that is a relative of wheat, aegilops speltoides, a 301-bp repeat containing 16 copies of a caa microsatellite was isolated. southern blot and fluorescence in situ hybridization revealed that approximately 250 bp of the sequence is tandemly arrayed at the centromere regions of a- and b-genome chromosomes of common wheat and rye chromosomes. although the dna sequence of this 250-bp repeat showed no notable homology in the databases, the flanking or intervening sequence ...012807787
map-based cloning of leaf rust resistance gene lr21 from the large and polyploid genome of bread wheat.we report the map-based cloning of the leaf rust resistance gene lr21, previously mapped to a gene-rich region at the distal end of chromosome arm 1ds of bread wheat (triticum aestivum l.). molecular cloning of lr21 was facilitated by diploid/polyploid shuttle mapping strategy. cloning of lr21 was confirmed by genetic transformation and by a stably inherited resistance phenotype in transgenic plants. lr21 spans 4318 bp and encodes a 1080-amino-acid protein containing a conserved nucleotide-bindi ...200312807786
nitrogen use and carbon sequestered by corn rotations in the northern corn belt, u.s.diversified crop rotation may improve production efficiency, reduce fertilizer nitrogen (n) requirements for corn (zea mays l.), and increase soil carbon (c) storage. objectives were to determine effect of rotation and fertilizer n on soil c sequestration and n use. an experiment was started in 1990 on a barnes clay loam (u.s. soil taxonomy: fine-loamy, mixed, superactive, frigid calcic hapludoll) near brookings, sd. tillage systems for corn-soybean ( glycine max [l.] merr.) rotations were conve ...200112806069
effect of bioaccumulation of cadmium on biomass productivity, essential trace elements, chlorophyll biosynthesis, and macromolecules of wheat seedlings.soil contamination with heavy metals has become a worldwide problem, leading to losses in agricultural yield and hazardous human health effects as they enter the food chain. the present investigation was undertaken to examine the influence of cadmium (cd2+) on the wheat (triticum aestivum l.) plant. cd2+ accumulation and distribution in 3-wk-old seedlings grown in nutrient medium containing varying concentrations of cd2+ (control, 0.25, 0.50, 1.0, 2.5, and 5.0 mg/l) was monitored. the effect of ...200312794277
agrobacterium tumefaciens-mediated transformation of wheat using a superbinary vector and a polyamine-supplemented regeneration medium.immature embryo-derived calli of spring wheat (triticum aestivum l.) cv veery5 were transformed using agrobacterium tumefaciens strain lba4404 carrying either binary vector phk22 or superbinary vector phk21, the latter carrying an extra set of vir genes--vir b, -c and -g. in both cases, transient beta-glucuronidase ( gus) expression ranging from 35-63% was observed 3 days after co-cultivation, but 587 calli infected with phk22/lba4404 failed to produce a single stably transformed plant, whereas ...200312789445
factors influencing successful agrobacterium-mediated genetic transformation of wheat.the development of a robust agrobacterium-mediated transformation protocol for a recalcitrant species like bread wheat requires the identification and optimisation of the factors affecting t-dna delivery and plant regeneration. we have used immature embryos from range of wheat varieties and the agrobacterium strain agl1 harbouring the pgreen-based plasmid pal156, which contains a t-dna incorporating the bar gene and a modified uida (beta-glucuronidase) gene, to investigate and optimise major t-d ...200312789416
molecular cloning and characterization of cdnas encoding hemoglobin from wheat (triticum aestivum) and potato (solanum tuberosum).hemoglobins (hbs) are heme proteins encountered in all five kingdoms of living organisms. in plants, two different classes of hbs have been identified: nonsymbiotic (class i) from both monocot and dicot species and symbiotic (class ii) hbs from nitrogen-fixing plants. this work reports the cloning and analysis of three nonsymbiotic hb genes from wheat (triticum aestivum) and potato (solanum tuberosum). the hb cdnas were amplified by reverse transcriptase polymerase chain reaction (rt-pcr) using ...200312787929
vernalization-induced flowering in wheat is mediated by a lectin-like gene ver2.a vernalization-related gene ver2 was isolated from winter wheat ( triticum aestivum l.) using a differential screening approach. the deduced ver2 is a lectin-like protein of 300 amino acids, which contains the presence of a jacalin-like gwg domain. rna in situ hybridization results demonstrated that ver2 gene expression is restricted to the marginal meristems of immature leaves in vernalized wheat seedlings. no hybridization signal was detected in the epidermal tissue and vascular bundles. howe ...200312783334
localization of sucrose synthase in wheat roots: increased in situ activity of sucrose synthase correlates with cell wall thickening by cellulose deposition under hypoxia.sucrose synthase (susy; ec 2.4.1.13) plays a prominent role in o(2) deficiency and functions at a branch point, partitioning sucrose between cell wall biosynthesis and glycolysis. the cleavage of sucrose by susy was localized in wheat ( triticum aestivum l. cv. alcedo) roots subjected to 4 days of hypoxia. increased susy activity was observed by in situ activity staining in the tip region and in the stele of root axes. the pattern of cellulose deposition correlated with regions of high susy acti ...200312783333
inheritance and localisation of resistance to mycosphaerella graminicola causing septoria tritici blotch and plant height in the wheat (triticum aestivum l.) genome with dna markers.resistance to the disease septoria tritici blotch of wheat (triticum aestivum l.), caused by the fungus mycosphaerella graminicola (fuckel.) j. schrot in cohn (anamorph septoria tritici roberge in desmaz.) was investigated in a doubled-haploid (dh) population of a cross between the susceptible winter wheat cultivar savannah and the resistant cultivar senat. a molecular linkage map of the population was constructed including 76 ssr loci and 244 aflp loci. parents and dh progeny were tested for re ...200312783168
deoxynivalenol and other fusarium toxins in wheat and rye flours on the danish market.information on the contamination of danish cereals and cereal products with fusarium toxins is limited and the last survey is from 1984/1985. in the present study, the occurrence of deoxynivalenol (don), nivalenol (niv), ht-2 toxin, t-2 toxin and zearalenone (zon) was investigated in flour of common wheat, durum wheat and rye. the samples were collected from 1998 to 2001 from both mills and the retail market in denmark. a total of 190 flour samples were analysed for don and niv and about 60 samp ...200312775483
cloning and characterization of two full-length cdnas, taga1 and taga2, encoding g-protein alpha subunits expressed differentially in wheat genome.in the present study, we identified and characterized two cdnas, named taga1 and taga2, encoding alpha subunits of heterotrimeric g proteins synthesized from one-week-old seedling mrnas of common wheat cv. s615 using race pcr and rt-pcr methods. the clone taga1 contained an open reading frame that encoded a protein consisting of 383 amino acid residues with a molecular mass of 51.3 kda, whereas the clone taga2 contained an open reading frame encoding 390 amino acids with a molecular mass of 52.5 ...200312773813
environmentally-induced changes in protein composition in developing grains of wheat are related to changes in total protein content.nitrogen (n) nutrition, post-anthesis temperature and drought-induced changes in the kinetics of accumulation of dry mass, total grain n and protein fractions (albumins-globulins, amphiphils, gliadins, and glutenins) contents were examined for winter wheat (triticum aestivum l.). crops were grown in controlled environment tunnels in 1994 and 1998. in 1994, five post-anthesis temperatures averaging from 15-25 degrees c were applied during grain-filling. in 1998 two post-anthesis temperatures aver ...200312773520
efficient clustering of large est data sets on parallel computers.clustering expressed sequence tags (ests) is a powerful strategy for gene identification, gene expression studies and identifying important genetic variations such as single nucleotide polymorphisms. to enable fast clustering of large-scale est data, we developed pace (for parallel clustering of ests), a software program for est clustering on parallel computers. in this paper, we report on the design and development of pace and its evaluation using arabidopsis ests. the novel features of our app ...200312771222
salicylic acid changes the properties of extracellular peroxidase activity secreted from wounded wheat (triticum aestivum l.) roots.wheat ( triticum aestivum l.) roots released proteins showing peroxidase activity in the apoplastic solution in response to wound stress. preincubation of excised roots with 1 mm salicylic acid at ph 7.0 enhanced the guaiacol peroxidase activity of the extracellular solution (so-called extracellular peroxidase). the soluble enzymes were partially purified by precipitation with ammonium sulfate followed by size exclusion and ion exchange chromatography. despite an increase in the total activity o ...200312768343
bread wheat gliadin cytotoxicity: a new three-dimensional cell model.in an attempt to clarify the role of gliadin toxicity in the pathogenesis of gluten intolerance (celiac disease), previous in vitro studies have been based on two-dimensional human cell cultures. however, the specific morphological and biochemical properties of in vivo tissue are better maintained in three-dimensional cell cultures (multicellular spheroids, mcs). the aim of this study was to develop a three-dimensional in vitro model to investigate the effects of gliadin on epithelial cells and ...200312751695
mapping qtls for seed dormancy and the vp1 homologue on chromosome 3a in wheat.a major component of the observed genetic variation for pre-harvest sprouting in wheat ( triticum aestivum l.) appears to be the level of seed dormancy. group 3 chromosomes have received attention as carrying the r genes for seed-coat color and the tavp1 genes that are orthologous to the maize vp1 gene which encode a dormancy-related transcription factor. the objectives of the present study were to map quantitative trait loci (qtls) for seed dormancy on chromosome 3a and to investigate an associ ...200312750793
powdery mildew resistance gene pm22 in cultivar virest is a member of the complex pm1 locus in common wheat ( triticum aestivum l. em thell.).the powdery mildew resistance gene pm22, identified in the italian wheat cultivar virest and originally assigned to wheat chromosome 1d, was mapped to chromosome 7a with the aid of molecular markers. mapping of common aflp and ssr markers in two wheat crosses segregating for pm22 and pm1c, respectively, indicated that pm22 is a member of the complex pm1 locus. pm22 also showed a pattern of resistance reaction to a differential set of blumeria graminis f. sp. tritici isolates that was distinguish ...200312750784
advanced backcross qtl analysis for the identification of quantitative trait loci alleles from wild relatives of wheat ( triticum aestivum l.).advanced backcross qtl (ab-qtl) analysis was used to identify quantitative trait loci (qtls) for yield and yield components in a bc(2)f(2) population derived from a cross between the german winter wheat variety 'prinz' and the synthetic wheat line w-7984 developed by cimmyt. two hundred and ten microsatellite markers were employed to genotype 72 pre-selected bc(2)f(2) plants and phenotypic data were collected for five agronomic traits from corresponding bc(2)f(3) families that were grown at four ...200312750781
a new hessian fly resistance gene (h30) transferred from the wild grass aegilops triuncialis to hexaploid wheat.a new hessian fly (mayetiola destructor say) resistance gene from aegilops triuncialis and its transfer to hexaploid wheat via interspecific hybridisation is described. the transfer line tr-3531 (42 chromosomes), derived from the cross [(triticum turgidum x ae. triuncialis) x triticum aestivum] and carrying the heterodera avenae resistance gene cre7, showed a high level of resistance to the m. destructor biotype prevailing in the sw of spain. a single dominant gene (h30) seems to determine the h ...200312748776
expression profiling and bioinformatic analyses of a novel stress-regulated multispanning transmembrane protein family from cereals and arabidopsis.cold acclimation is a multigenic trait that allows hardy plants to develop efficient tolerance mechanisms needed for winter survival. to determine the genetic nature of these mechanisms, several cold-responsive genes of unknown function were identified from cold-acclimated wheat (triticum aestivum). to identify the putative functions and structural features of these new genes, integrated genomic approaches of data mining, expression profiling, and bioinformatic predictions were used. the analyse ...012746512
evaluation of cytological effects of zn2+ in relation to germination and root growth of nigella sativa l. and triticum aestivum l.the effects of different treatments with zinc sulfate (zn(2+)) on the cytology and growth of nigella sativa and triticum aestivum were investigated. five concentrations of zinc sulfate ranging from 5 to 25mg/l were applied for 6, 12, 18, and 24h. the treatments reduced the germination percentages of n. sativa seeds and t. aestivum grains and inhibited the root growth of both plants. concentrations higher than 25mg/l of zn(2+) applied for 24h were toxic for both plants. the non-lethal concentrati ...200312742505
[hybrids of aegilops cylindrica host with triticum durum desf. and t. aestivum l].the hybrids of durum and bread wheat with ae. cylindrica have been obtained without using an embryo rescue technique. the hybrid output (of pollinated flower number) in the field conditions scored 1.0, 15.3 and 10.0% in the crosses t. durum x ae. cylindrica, ae. cylindrica x t. durum and t. aestivum x ae. cylindrica, respectively. a high level of meiotic chromosome pairing between homologous d genomes of bread wheat and aegilops has been revealed (c = 80.0-83.7%). the possibility of homoeologica ...200812741056
rearrangement of the genes for the biosynthesis of benzoxazinones in the evolution of triticeae species.gramineous plants, including the major agricultural crops wheat (triticum aestivum l.), rye (secale cereale l.) and maize (zea mays l.), accumulate benzoxazinones (bxs) as defensive compounds. previously, we isolated cdnas of the bx biosynthetic genes in wheat, tabx2- tabx5, that encode the enzymes catalyzing the sequential hydroxylation of indole to bxs. in this study we isolated a cdna of tabx1, which encodes the first enzyme of the bx pathway of wheat. the level of identity (80%) in deduced a ...200312734755
initial crop growth in soil collected from a closed animal waste lagoon.in the 21st century, remediation of the soil beneath animal waste lagoons will become an important issue, as they are closed due to environmental regulations or to abandonment. the possibility of growing crops in the soil, which has high concentrations of ammonium-n, has not been studied. the objective of this experiment was to determine if crop species would germinate and grow in lagoon soil. soil was gathered from a lagoon that had received wastes from swine (sus scrofa) and beef (bos taurus) ...200312733569
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