Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| herpes simplex virus type 2 encephalitis in two homosexual men with persistent lymphadenopathy. | within a 5-month period, 2 homosexual men with persistent lymphadenopathy developed clinical findings consistent with herpes simplex virus (hsv) encephalitis. these signs included abrupt change in mental status, seizures, cerebrospinal fluid pleocytosis, and localized electroencephalographic abnormalities showing temporal lobe involvement. initial computed tomographic scans were unremarkable. treatment with adenine arabinoside was instituted and temporal lobe biopsies were performed. although vi ... | 1985 | 2983602 |
| a prospective study of herpes simplex virus infection in a defined population in houston, texas. | a seroepidemiologic study was conducted to determine the incidence of antibodies to herpes simplex virus in a population of middle-class caucasian women. in utero exposure to diethylstilbestrol did not influence the frequency of herpes simplex virus infection. the frequency of infection increased with age and number of sex partners and a 50% increase in incidence of herpes simplex virus type 2 infection was noted during the 4-year period of the study. subclinical herpes simplex virus type 2 infe ... | 1985 | 2983557 |
| protection from genital herpes simplex virus type 2 infection by vaccination with cloned type 1 glycoprotein d. | guinea pigs were vaccinated with truncated herpes simplex virus type-1 (hsv-1) glycoprotein d produced in the genetically engineered mammalian cell line gd10.2. vaccinated animals formed antibodies that neutralized both hsv-1 and herpes simplex virus type 2 (hsv-2) in an in vitro neutralization assay. vaccinated animals were challenged with hsv-2 by intravaginal infection. animals that received the immunogen in freund's complete adjuvant were completely protected from the clinical manifestations ... | 1985 | 2983428 |
| the herpes simplex virus amplicon: analyses of cis-acting replication functions. | previous studies have shown that defective virus vectors (amplicons) derived from herpes simplex viruses could be efficiently propagated in virus stocks in the presence of trans-acting helper virus functions. the present study established that two separate cis-acting functions--a dna replication origin and a cleavage/packaging signal--are required for amplicon propagation. using deleted derivatives of cloned amplicons, we mapped one of the viral dna replication origins (ori-2 or oril) at coordin ... | 1985 | 2983310 |
| evidence for a direct role for both the 175,000- and 110,000-molecular-weight immediate-early proteins of herpes simplex virus in the transactivation of delayed-early promoters. | we reconstructed the regulated induction of delayed-early (de) transcription that occurs during herpes simplex virus (hsv) infection by using a transient expression system in which recombinant target genes were cotransfected into vero cells together with intact activating genes. plasmids containing cloned hsv-1 or hsv-2 immediate-early (ie) genes stimulated by up to 100-fold the expression from recombinant constructs containing the bacterial chloramphenicol acetyltransferase (cat) gene under the ... | 1985 | 2983086 |
| herpes simplex virus sequences involved in the initiation of oncogenic morphological transformation of rat cells are not required for maintenance of the transformed state. | we have determined the herpes simplex virus (hsv) type 2 dna sequences responsible for the initiation of morphological transformation and have investigated the retention and expression of these sequences in morphologically transformed cells and in tumours derived from these cells. all the transformed cells analysed were selected by a focus formation assay and are oncogenic in the inbred host rat. cloned hindiii and bg/ii fragments from the hsv-2 genome were assayed for the ability to initiate mo ... | 1985 | 2983000 |
| acute and latent infection by thymidine kinase mutants of herpes simplex virus type 2. | 1985 | 2982968 | |
| herpes simplex virus type 1 infection of endothelial, epithelial, and fibroblast cells induces a receptor for c3b. | we recently demonstrated that herpes simplex virus type 1 (hsv 1) induces a receptor on human umbilical vein endothelial cells for complement component c3b (c3br). we assigned this receptor function to hsv 1 viral glycoprotein c (gc) based on several observations: tunicamycin, which prevents glycosylation and expression of n-linked glycoproteins on the surface of infected cells, markedly reduced expression of the c3br; monoclonal antibodies to hsv 1 gc blocked detection of the c3br, whereas mono ... | 1985 | 2982950 |
| antiviral activity of cp-20,961 against herpes simplex viruses in vitro. | the antiherpesvirus activity of cp-20,961 [n,n-dioctadecyl-n',n'-bis (2-hydroxyethyl) propanediamine, or avridine] was investigated in cultured guinea pig embryo (gpe) cells. plaque formation of herpes simplex virus type 1 (hsv1) and type 2 (hsv2) was inhibited, but vesicular stomatitis virus replication was not inhibited, in gpe cells treated with cp-20,961 before infection. the id50 concentration of cp-20,961 for hsv was about 50 micrograms/ml for 3-4 days of pretreatment. after virus adsorpti ... | 1985 | 2982759 |
| herpes simplex virus latency in a hyperresistant clone of mouse neuroblastoma (cl300) cells. | herpes simplex virus (hsv) hyperresistant neuroblastoma cells (clone cl300 rii) were latently infected with hsv-1 if cultured in presence of hsv-neutralizing antibody for one or two passages after infection and then passaged further without antibody. by superinfecting hsv-1 latently infected cl300 rii cells with hsv-2, progeny virus with hsv-1 characteristics was regularly rescued. such retrieval of hsv-1 decreased with passage of the latently infected cells. | 1985 | 2982359 |
| characterization of the gene encoding herpes simplex virus type 2 glycoprotein c and comparison with the type 1 counterpart. | the gene encoding the glycoprotein c (gc) of herpes simplex virus type 1 maps to the region of the viral genome from 0.62 to 0.64. recently, a herpes simplex virus type 2 glycoprotein previously designated gf and now designated gc was mapped to a homologous location. analysis of the herpes simplex virus type 2 mrna species encoded in this region revealed a major transcript of 2.5 kilobases, a 0.73-kilobase transcript (the 5' ends of which were mapped by primer extension), and several minor speci ... | 1985 | 2982036 |
| increased mutation frequency after herpes simplex virus type 2 infection in non-permissive xc cells. | the effect of herpes simplex virus type 2 (hsv-2) infection on the frequency of mutations at the hypoxanthine-guanine phosphoribosyl transferase (hgprt) locus was studied in the non-permissive xc cell line. when the cells were infected with 20 to 800 p.f.u./cell, there was initially a lag in cell growth and cell death, but after 4 days there was no difference in growth rate between infected and control cultures. however, the mutation frequency, as determined by the number of 6-thioguanine-resist ... | 1985 | 2981968 |
| antigenic and biochemical analysis of gb of herpes simplex virus type 1 and type 2 and of cross-reacting glycoproteins induced by bovine mammillitis virus and equine herpesvirus type 1. | an antiserum was produced to the oligomeric form of glycoprotein b (gb) induced by herpes simplex virus type 1 (hsv-1) strain 17. this antiserum gave a single common precipitin line in agar gel immunodiffusion with hsv-1, hsv-2, bovine mammillitis virus (bmv) and equine herpesvirus type 1 (ehv-1). it also neutralized hsv-1, hsv-2 and bmv but not ehv-1. absorption of the antiserum with excess hsv-2 or bmv antigen resulted in an hsv-1-specific neutralizing antiserum. in immunoprecipitation, two pr ... | 1985 | 2981966 |
| herpes simplex virus type 2 and human papillomavirus type 16 in cervicitis, dysplasia and invasive cervical carcinoma. | sera and biopsies of cervical lesions from 55 women with benign or malignant disease were analyzed for evidence of infection with herpes simplex virus type 2 (hsv-2) or human papillomavirus (hpv). in addition, information regarding known risk factors for cervical cancer was obtained by interview. the sera were tested for hsv-2 antibodies and the biopsies were tested for hpv or hsv dna sequences by southern blot hybridization. hsv-2 sequences were detected in 2/13 (15%) invasive neoplasms and in ... | 1985 | 2981783 |
| a prospective study of association of herpes simplex virus and human papillomavirus infection with cervical neoplasia in women exposed to diethylstilbestrol in utero. | one thousand one hundred thirty-four women, enrolled in a prospective study of the development of cervical neoplasia in women exposed to diethylstilbestrol (des) in utero, were examined at least once a year. twenty-three women developed cervical intraepithelial neoplasia (cin) of different degrees during the 7-year follow-up period and were matched with women who did not develop cin during the study period. serum samples were obtained at the time of entry into the study and at the time of diagno ... | 1985 | 2981778 |
| antiviral activity of 5'-paa and 5'-pfa phosphate esters of 2'-deoxyuridines. | the 5'-paa and 5'-pfa phosphate esters of 5-bromo-2'-deoxyuridine (budr) were synthesized and their antiherpes virus activity was evaluated in vitro. both compounds showed activity of the same order as the parent nucleoside, budr, against hsv-2 but were 4 to 12 times less potent against hsv-1. the 5'-paa phosphate ester of budr (ro 21-9875) was also active against varicella-zoster virus (vzv) and human cytomegalovirus (hcmv). the 5'-paa phosphate ester of 5-bromovinyl-2'-deoxyuridine (bvdu) was ... | 1986 | 2947889 |
| [herpes simplex virus infection of the central nervous system]. | herpes simplex virus type 1 (hsv 1) infection in the central nervous system results in encephalitis with high mortality and morbidity. however herpes simplex virus type 2 (hsv 2) infection in this area usually results in benign meningitis. brain biopsy is the most sensitive and most specific method for diagnosing hsv 1 encephalitis. antibody determinations in serum and cerebrospinal fluid give only a retrospective diagnosis. a rapid non-invasive diagnostic test is urgently required as an alterna ... | 1989 | 2919376 |
| [viral or bacterial meningitis. differential diagnostic problems]. | a 19 year old previously healthy man presented symptoms of meningitis. culture from the cerebrospinal fluid revealed herpes simplex virus type 2 to be the responsible agent. the course was benign, the patient was never seriously ill. he recovered fully within a few weeks. the cerebrospinal fluid showed prolonged pleocytosis and low values of glucose. the traditional cerebrospinal fluid parameters employed in the diagnosis of meningitis, and for distinguishing bacterial from viral meningitis, are ... | 1989 | 2919375 |
| acyclic analogues of 3'-azido-3'-deoxythymidine as potential antiviral agents. nucleoside synthesis by michael addition. | an acyclic derivative of 3'-azido-3'-deoxythymidine, (r,s)-1-[1-(2-hydroxyethoxy)-3-azidopropyl]thymine (2), has been synthesized by a path involving michael-type addition. related thymidine analogues lacking the c(3')-c(4') bond were similarly obtained. the method provides a versatile new route to nucleoside analogues. the new compounds were found to be essentially inactive against human immunodeficiency virus type 1 (hiv-1) and herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in vitro wh ... | 1989 | 2909745 |
| components required for in vitro cleavage and polyadenylation of eukaryotic mrna. | we have studied in vitro cleavage/polyadenylation of precursor rna containing herpes simplex virus type 2 poly a site sequences and have analyzed four rna/protein complexes which form during in vitro reactions. two complexes, a and b, form extremely rapidly and are then progressively replaced by a third complex, c which is produced following cleavage and polyadenylation of precursor rna. substitution of atp with cordycepin triphosphate prevents polyadenylation and the formation of complex c howe ... | 1988 | 2898767 |
| difference between herpes simplex virus type 1 and type 2 neonatal encephalitis in neurological outcome. | 24 infants consecutively treated with acyclovir or vidarabine for neonatal herpes simplex virus (hsv) encephalitis were followed up for 6 months to 3 years to assess neurological and developmental outcome. 15 patients had hsv-2 and 9 had hsv-1 encephalitis. infants with hsv-2 encephalitis presented with a higher frequency of seizures, greater pleocytosis and protein concentrations in the cerebrospinal fluid, and more frequent evidence of structural damage on computerised tomographic scans of the ... | 1988 | 2891886 |
| high levels of cytomegalovirus antibody in patients requiring vascular surgery for atherosclerosis. | 157 caucasian male patients undergoing vascular surgery for atherosclerosis and a matched control group of patients with high cholesterol levels were screened for antibodies to cytomegalovirus (cmv) and herpes simplex virus type 1 (hsv1) and type 2 (hsv2), indicative of persistent infection. the prevalence of cmv antibodies was higher in the surgical group than in the control group (90% and 74%, respectively), and a significantly greater percentage (p less than 0.001) of surgical cases than cont ... | 1987 | 2886763 |
| studies of the major dna binding proteins of two bovine herpes mammillitis virus isolates. | antigenic cross-reaction of the dna-binding protein between herpes simplex virus type 2 (hsv-2) and two bovine herpes mammillitis virus (bhmv) isolates (bhm-1 and bhmv csiro 290) was demonstrated by indirect immunofluorescence. southern blot analysis showed a region of homology between hsv-2 bglii o dna (coding for the hsv-2 major dna-binding protein) and bhmv xbai dna fragment n, suggesting a similar gene map unit position on both genomes. | 1987 | 2886026 |
| transcriptional induction of cellular gene expression during lytic infection with herpes simplex virus. | herpes simplex virus type 2 causes a severe repression of host cell biosynthesis at a number of levels. we show that despite this, non-viral cdna clones derived from cellular rna species which accumulate to high levels after infection can be isolated using differential screening techniques. by using nuclear run-off assays, we have shown that this rna accumulation is mediated by transcriptional induction of the corresponding cellular genes. | 1986 | 2883998 |
| comparative polypeptide analysis of human, murine and strigis herpesviruses with murine cytomegalovirus by polyacrylamide gel electrophoresis. | the polypeptide composition of five purified murine herpesvirus (mhv) strains grown in a stable line of rabbit embryo fibroblasts (ref) was analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) and compared with herpes simplex virus type 1 (hsv-1). about 24 structural polypeptides of molecular mass ranging from 275,000 to 25,000 were identified in mhv and hsv-1. the polypeptide profiles of mhv and hsv-1, showed a close similarity. the polypeptides of mhv were further ... | 1987 | 2883851 |
| induction of chromosomal aberrations in human cells by a temperature-sensitive mutant of herpes simplex virus type 2 and its revertants. | the induction of chromosomal aberrations by a temperature-sensitive (ts) mutant of herpes simplex virus type 2 (hsv-2) strain hg52 (ts 13), its revertants 4-8 and 5-8 and by etalon strains hsv-1 17 syn+ and hsv-2 hg52 was studied in human fibroblast and lymphocyte cultures. the effect on chromosomes of the revertants was tested at permissive (31 degrees c) and non-permissive (38 degrees c) temperatures. at 38 degrees c the revertants could not induce dnase activity. the present results contribut ... | 1986 | 2874732 |
| early intrathecal production of specific igm and igg antibodies and alpha-interferon in herpes simplex virus encephalitis. | a complex approach was used in order to establish non-invasively the aetiology in three cases of encephalitis presumptively caused by herpes simplex virus (hsv). as indicative of brain hsv infection was considered the lowered serum to cerebrospinal fluid specific antibody ratio, which also assessed the humoral immune response within the cns. for this purpose, during ongoing brain tissue infection, the early intrathecal (ith) production of igg and igm antibodies was analysed by a differential enz ... | 1986 | 2873733 |
| studies on interactions of dtk-hsv mutants with neurons in vitro. | thymidine kinase negative (dtk-) mutants of herpes simplex virus type 1 (hsv-1) multiplied well in rat brain glioma cells. a proportion (less than 1%) of glioma cells survived the infection with hsv and were designated "survivor" glioma cells. survivor cells of dtk- mutant virus infection ceased to produce infectious virus after two passages and were highly resistant to both hsv-1 and hsv-2 but not to vesicular stomatitis virus (vsv). flow cytometric studies indicated morphological differences b ... | 1986 | 2871727 |
| induction of recurrent genital herpes simplex virus type 2 infection by ultraviolet light. | 1986 | 2870285 | |
| herpes simplex virus hepatitis in mice: effects of treatment with trisodium phosphonoformate. | clinical isolates of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) induced focal necrotic hepatitis in icr/schö mice. the extent of the hepatitis could be considerably reduced by trisodium phosphonoformate (pfa) administered for 3 days in a daily dose of 400-600 mg/kg. the hsv hepatitis in mice could serve as a suitable model for testing antiherpetic drugs. | 1985 | 2869661 |
| anti-herpes simplex virus and cytostatic activity of some new 5-substituted 1-(4-hydroxybutyl)-and 1-(2-hydroxyethoxymethyl) uracil nucleoside analogues. | two series of 5-substituted acyclic uracil nucleoside analogues (5-x-acyclo-u) were evaluated for their inhibitory effects against three herpes simplex virus type 1 (hsv-1) strains and one type 2 (hsv-2) strain in a plaque inhibition assay on human embryonic lung fibroblast (helf) cell cultures as well as for their ability to inhibit the proliferation of baby hamster kidney cells in suspension (bhk-s) culture. acyclovir [9-(2-hydroxyethoxymethyl)guanine; acv] and (s)-9-(2,3-dihydroxypropyl)adeni ... | 1985 | 2864819 |
| [herpes simplex virus type 2 antibody levels in sera from various age groups using elisa and ifat]. | we have investigated hsv-2, igg antibody levels in 180 sera which were obtained from 60 girl students between 18-20 years old, 60 pregnant women between 19-39 years old and 60 young men between 20-22 years old, by using elisa (enzyme linked immunosorbent assay) and ifat (indirect fluorescent antibody technique). all of the sera obtained from girl students and pregnant women were found negative for hsv-2, igg antibody by elisa. however 2 of 60 young men's sera were positive. totally 2 of 180 sera ... | 1988 | 2856217 |
| vaccine induced hsv 1 antibodies fail to correlate with protection against hsv 2 in guinea pigs. | guinea pigs immunised with hsv 1 subunit vaccine were bled, and subsequently challenged intravaginally with hsv 2. the clinical response to virus challenge was quantified, and correlations were sought between clinical score and virus-specific serum antibody titre in functional and binding assays (elisa, neutralization, complement-mediated cytotoxicity and antibody-dependent cellular cytotoxicity). no significant relationships were found, and it was concluded that reactivity in the serological as ... | 1988 | 2856207 |
| efficacy of 5-vinyl-1-beta-d-arabinofuranosyluracil (varau) against herpes simplex virus type 2 strains in cell cultures and against experimental herpes encephalitis in mice: comparison with acyclovir and foscarnet. | the sensitivity of different herpes simplex virus type 2 (hsv-2) strains to inhibition by 5-vinyl-1-beta-d-arabinofuranosyluracil (varau) was evaluated in comparison to 9-(2-hydroxyethoxymethyl)guanine (acv; acyclovir) and trisodiumphosphonoformate (na3pfa; foscarnet), using a plaque inhibition assay in primary rabbit testes (prt) cells as well as in human embryonic lung fibroblast (helf) cell cultures. the order of decreasing activity found was acv much greater than varau greater than na3pfa in ... | 1987 | 2855541 |
| antiherpes activity of the immunomodulator ok-432, a streptococcal preparation, in immunosuppressed mice. | the antiviral activity of ok-432, an antitumor agent originating from streptococcal preparations, against herpes simplex virus type 2 (hsv-2) was investigated in mice immunosuppressed by cyclophosphamide (cy). intraperitoneal administration of ok-432 to mice 1 day after treatment with 200 mg cy/kg prevented death due to hsv-2 encephalitis in a dose-dependent manner. when the immunosuppressed mice were given ok-432 prior to hsv-2 infection, both by the intraperitoneal route, virus growth in the p ... | 1988 | 2854959 |
| antiviral activities of 2'-deoxyribofuranosyl and arabinofuranosyl analogs of sangivamycin against retro- and dna viruses. | eight sugar-modified pyrrolopyrimidine nucleoside analogs related to the antibiotic sangivamycin were evaluated in cell culture against herpes simplex types 1 (hsv-1) and 2 (hsv-2), cytomegalovirus (cmv), adenovirus, and visna virus. five of the compounds were highly active against most of the viruses with 50% inhibition (ed50) values of 1-10 microm. the selectivity of the agents was low, with inhibition of uninfected cell proliferation occurring within 5-fold that of the virus ed50 for most of ... | 1988 | 2854957 |
| vaccine potential of a live avirulent herpes simplex virus. | we previously described a herpes simplex intertypic recombinant that is completely and specifically non-neurovirulent following intracranial inoculation. in this report the vaccine potential of this mutant (re6) is analyzed. it is shown that re6 replicates to wild type levels at the peripheral site of inoculation (the mouse footpad) but, unlike its virulent parental strains, does not replicate to detectable levels in lumbosacral ganglia. a single vaccination with re6 is sufficient to completely ... | 1986 | 2854601 |
| dna sequence and rna transcription through a site of recombination in a non-neurovirulent herpes simplex virus intertypic recombinant. | re6 is a herpes simplex type-1 (hsv-1) x herpes simplex type-2 (hsv-2) intertypic recombinant that cannot replicate in the adult mouse nervous system. in the accompanying report, we have shown that hsv-1 sequences between 0.698 and 0.721 map units can restore a partial neurovirulent phenotype to re6. in this report, we have used comparative dna sequence analysis of re6, 17syn+ (hsv-1) and hg52 (hsv-2) to demonstrate that this region contains a site of recombination between hsv-1 and hsv-2 sequen ... | 1988 | 2854325 |
| cervical carcinoma: an experimental approach. | a mouse model system was used to investigate the preventive efficacy of a subunit herpes simplex virus (hsv) vaccine on the development of hsv induced cervical carcinoma. ten groups of mice were vaccinated before receiving repeated intravaginal exposure to hsv-type 2 inactivated by ultraviolet irradiation. at 20 months postvaccination, neutralizing antibody activity to herpes simplex viruses was detected in the sera of the mice which had received the highest vaccine dose. although three experime ... | 1988 | 2853998 |
| the aetiological relationship between herpes simplex virus type 2 and carcinoma of the cervix: an unanswered or unanswerable question? | after two decades of investigation the proposed aetiological link between herpes simplex virus type 2 (hsv2) and human carcinoma of the cervix remains unproved. the initial seroepidemiological studies, which were of a retrospective nature, were in favour of the aetiological relationship, whereas the more recent prospective studies suggest that hsv infection is a co-variable. the detection of hsv genes and their products in tumour tissues has been plagued by problems of cross reactivity, but the ... | 1988 | 2853997 |
| homology of the hsv-2 "a-sequence" to cellular sequences. | bgl-ii fragments of the genome of herpes simplex virus type 2 (hsv-2) hg-52 were cloned into the vector p-neo and were used to screen the complete hsv-2 genome for regions cross-hybridizing with the genome of hel cells. most extensive cross-hybridizing activity was observed with a 530 bp sstii subfragment of the viral bamhi g dna-fragment (contained in bgl ii f), which spans the joint and the viral a-sequence. from a lambda-l47 library, a cellular 15 kb hindiii dna fragment was subcloned in pbr ... | 1988 | 2853486 |
| herpes simplex virus glycoprotein treatment of recurrent genital herpes reduces cervicovaginal virus shedding in guinea pigs. | treatment of previously infected guinea pigs with herpes simplex virus (hsv) glycoproteins reduces the frequency and severity of subsequent genital recurrences. an effective vaccine should also reduce episodes of viral shedding. in this study, hsv glycoproteins b and d treatment of animals experiencing recurrent genital herpes reduced the frequency of both clinical recurrences and cervicovaginal viral shedding. when virus was shed, however, the peak viral titer and duration of shedding was unalt ... | 1988 | 2852922 |
| [seroepidemiological survey of herpes simplex virus type 2 in cervical erosion women]. | 1988 | 2852541 | |
| broad-spectrum antiviral activity of the acyclic guanosine phosphonate (r,s)-hpmpg. | (r,s)-9-(3-hydroxy-2-phosphonomethoxypropyl)guanine [(r,s)-hpmpg] exhibits broad spectrum antiviral activity with an ed50 of less than 1 microm against herpes simplex virus (hsv) types 1 and 2, varicella zoster virus, human cytomegalovirus (hcmv) and vaccinia in plaque reduction assays. wild type hsv-2 and its thymidine kinase deficient variant are equally sensitive to (r,s)-hpmpg. (r,s)-hpmpg is 100-fold more potent than acyclovir (ed50 = 0.45 microm vs. 44 microm, respectively) against hcmv in ... | 1988 | 2852486 |
| does neoplasia in-situ develop due to the interaction of epstein-barr virus or herpes simplex virus-2 with langerhans cells in the epithelium? | 1988 | 2852413 | |
| secretory iga against herpes simplex virus in cervical secretions. | a recently developed method to recover proteins from cervical secretions was combined with methods to detect minute concentrations of herpes simplex virus (hsv) type specific antibodies to measure the concentrations of locally produced antibodies in women with genital infections. forty nine women attending a sexually transmitted disease (std) clinic were included. cervical secretions were obtained by suction into a plastic catheter. soluble proteins were recovered from the secretions by elution ... | 1988 | 2852157 |
| the use of colloidal gold immunoelectron microscopy to diagnose varicella-zoster virus (vzv) infections by rapid discrimination between vzv, hsv-1 and hsv-2. | colloidal gold immunoelectron microscopy was used to diagnose rapidly 53 cases clinically suspected of varicella-zoster virus (vzv) infection and one special case selected from another study on typical herpes simplex virus (hsv) infections. the viruses were identified and subsequently typed within 2.5 h by a direct labelling test for vzv, and within 3.5 h by an indirect labelling test with monoclonal antibodies against hsv type 1 and type 2. the protein a purified igg fraction of human anti-vzv ... | 1988 | 2851604 |
| presence of insulin binding sites on viral particles. | using a standard radio-receptor assay, we have demonstrated that [125i]insulin can bind specifically to each of two types of purified enveloped viruses, influenza a virus and rous sarcoma virus. a non-enveloped icosahedral virus (echovirus 11) and herpes simplex virus type 2, which acquires its envelope from the nuclear membrane of the cell, did not possess insulin receptor activity. displacement of specifically bound radiolabelled insulin from the viral surface was achieved by addition of an ex ... | 1988 | 2851292 |
| electron microscopic localization of acridine orange chromatin interaction products in cells transformed by herpes simplex virus type 2. | the purpose of this study was to examine the distribution pattern of acridine orange (ao) binding to dna in transformed cells and derived tumor cells. two cell clones from hamster embryonic fibroblasts (hef) transformed by herpes simplex virus type 2, tumor cells derived from the transformed cell clones, and normal hef in culture were processed for ultracytochemistry. uptake studies and autoradiography using [3h] thymidine and [3h]-uridine were also performed. ultrastructural examination reveale ... | 1988 | 2851256 |
| neurovirulence of individual plaque stocks of herpes simplex virus type 2 strain hg 52. | the virulence of ten independent plaque stocks of hsv-2 strain hg 52 was determined by intracranial inoculation of 3 week old balb/c mice. the ten stocks exhibited a range of ld50 which could be grouped into three classes; high (less than 10(3) pfu/mouse), intermediate (10(3)-10(4) pfu/mouse) and low (greater than 10(5) pfu/mouse). analysis of the ten stocks using six different restriction endonucleases showed no significant variation in the size and distribution of fragments. isolates from the ... | 1988 | 2850778 |
| herpes simplex virus induces the replication of foreign dna. | plasmids containing the simian virus 40 (sv40) dna replication origin and the large t gene are replicated efficiently in vero monkey cells but not in rabbit skin cells. efficient replication of the plasmids was observed in rabbit skin cells infected with herpes simplex virus type 1 (hsv-1) and hsv-2. the hsv-induced replication required the large t antigen and the sv40 replication origin. however, it produced concatemeric molecules resembling replicative intermediates of hsv dna and was sensitiv ... | 1988 | 2850486 |
| interaction of herpes simplex virus type 2 with a rat glioma cell line. | the interaction between herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and two neural cell lines, mouse neuroblastoma (n1e-115) and rat glioma (c6-bu-1), was investigated. n1e-115 cells were permissive to both types of hsv. in c6-bu-1 cells, on the other hand, all the hsv-1 strains tested so far showed persistent infection, and the infectious virus of hsv-2 strains disappeared spontaneously. the hsv-2-infected c6-bu-1 cells were positive for hsv-2-specific dna sequences, virus-specific r ... | 1988 | 2850449 |
| complement component c3b binds directly to purified glycoprotein c of herpes simplex virus types 1 and 2. | cells infected with herpes simplex virus type 1 (hsv-1), but not hsv-2, express on their surfaces a receptor for the complement component c3b. receptor activity is markedly enhanced by treatment of the infected cells with neuraminidase. employing a direct binding assay, consisting of purified hsv glycoproteins immobilized on nitrocellulose and iodinated c3b as a probe, we found that c3b binds directly to gc-1, as well as to gc-2, but not to gb or gd from either serotype. c3b binding was enhanced ... | 1987 | 2849025 |
| chronic-dose acyclovir to suppress frequently recurring genital herpes simplex virus infection: effect on antibody response to herpes simplex virus type 2 proteins. | to determine the effect of prolonged suppressive acyclovir therapy on the antibody response to herpes simplex virus type 2 (hsv-2) proteins, we studied sequential sera from 33 patients with frequently recurring (six or more recurrences per year) genital herpes. twenty-two patients received 400 mg of oral acyclovir and 11 received placebo, twice daily for one year. sera collected at enrollment, after six months and 12 months of therapy, and during the first recurrence after cessation of therapy w ... | 1988 | 2848900 |
| ability of herpes simplex virus (hsv) types 1 and 2 to induce clinical disease and establish latency following previous genital infection with the heterologous hsv type. | guinea pigs were infected intravaginally with either herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2). one month postinoculation, animals were inoculated with the heterologous hsv type and observed for clinical disease and shedding of virus. one month after superinfection, animals were killed, and tissues were cocultivated to detect latent virus. although the severity of clinical disease and the degree of shedding of virus were greatly reduced by prior infection with the heterologous virus ... | 1988 | 2848899 |
| human papillomavirus & herpes simplex virus type 2 dna sequences with chronic cervicitis & invasive cervical cancer. | 1988 | 2848763 | |
| correlation of histologic types of carcinoma of the uterine cervix and human papillomavirus and herpes simplex virus type 2 dna sequences in the uterine cervical biopsies. | forty patients with invasive cancer of cervix from new delhi were examined for the presence of human papillomavirus (hpv) and herpes simplex virus (hsv) type 2 dna sequences in a southern hybridization assay. data were analysed according to the histologic type of cancer. hpv dna sequences were detected in 82.5% biopsies and hsv-2 dna sequences were found in 10% biopsies. hpv-16 dna sequences were found either alone or together with hpv-18 and/or hsv-2 bgl ii n fragment in 55% biopsies from kerat ... | 1988 | 2848476 |
| selective inhibition of human cytomegalovirus replication by a novel nucleoside, oxetanocin g. | a novel nucleoside with an oxetanosyl-n-glycoside has been recently isolated from a culture filtrate from bacillus megaterium and named oxetanocin a (n. shimada, s. hasegawa, t. harada, t. tomisawa, a. fujii, and t. takita, j. antibiot. 39:1623-1625, 1986). in this study, we evaluated the antiherpesvirus activity of oxetanocin a and its derivatives and found that 9-(2-deoxy-2-hydroxymethyl-beta-d-erythro-oxetanosyl)guanine (oxt-g) was very potent and selective in inhibiting the replication of hu ... | 1988 | 2847638 |
| effects of various nucleosides on antiviral activity and metabolism of 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl)uracil against herpes simplex virus types 1 and 2. | uptake of 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl)uracil (bv-arau) into herpes simplex virus type 1 (hsv-1)- and 2 (hsv-2)-infected cells was elevated about 190 to 40 times, compared with that into mock-infected human embryo lung fibroblast cells. uptake was not enhanced by infection with thymidine kinase-negative hsv-1 and hsv-2 mutants, however. in hsv-1-infected cells, 9.7% of bv-arau was phosphorylated to bv-arau triphosphate, but only 1.1% was phosphorylated in hsv-2-infected cells. the ... | 1988 | 2847634 |
| combined herpes simplex virus type 2 and human papillomavirus type 16 or 18 deoxyribonucleic acid leads to oncogenic transformation. | tumorigenic transformation was produced by combining herpes simplex virus type 2 and human papillomavirus type 16 or 18 deoxyribonucleic acid (each is associated with cervical carcinoma). recombinant human papillomavirus type 16 and 18 deoxyribonucleic acid each containing a head-to-tail dimer of the full-length human papillomavirus respective genomes, induced morphologic transformation in an immortalized cell line (ae), obtained by transfection of syrian hamster embryo cells with immortalizing ... | 1988 | 2847531 |
| the cleavage recognition signal is contained within sequences surrounding an a-a junction in herpes simplex virus dna. | herpesvirus genome maturation involves site-specific cleavage of viral dna concatemers and encapsidation of unit-length molecules, processes that are apparently coupled. here, applying a transfection-infection approach, we have investigated the arrangement of the dna sequence elements involved in cleavage and shown that specific cleavage occurs independently of dna replication. we show that the cis-acting signal for cleavage is located within a 179-bp fragment from across an a-a junction formed ... | 1988 | 2847414 |
| properties of two 5'-coterminal rnas transcribed part way and across the s component origin of dna synthesis of the herpes simplex virus 1 genome. | in cells infected with herpes simplex virus 1 the domains of the two identical origins of viral dna synthesis in the s component (oris) are transcribed in two 5'-coterminal rnas. orisrna1, synthesized early in infection and also in the absence of protein synthesis by infected cells, runs antisense to and terminates at the transcription initiation site for alpha genes 22 or 47. orisrna2, reported earlier, was detected in cells 14 hr after infection; it extends across the origin and is coterminal ... | 1988 | 2847162 |
| herpes simplex viruses lacking glycoprotein d are unable to inhibit virus penetration: quantitative evidence for virus-specific cell surface receptors. | herpes simplex virus (hsv) glycoprotein d (gd) plays an essential role in the entry of virus into cells. hsv mutants unable to express gd were constructed. the mutants can be propagated on vd60 cells, which supply the viruses with gd; however, virus particles lacking gd were produced in mutant-infected vero cells. virus particles with or without gd adsorbed to a large number (greater than 4 x 10(4] of sites on the cell surface; however, virions lacking gd did not enter cells. cells pretreated wi ... | 1988 | 2846873 |
| herpes simplex virus (hsv)-specific proliferative and cytotoxic t-cell responses in humans immunized with an hsv type 2 glycoprotein subunit vaccine. | studies were undertaken to determine whether immunization of humans with a herpes simplex virus type 2 (hsv-2) glycoprotein-subunit vaccine would result in the priming of both hsv-specific proliferating cells and cytotoxic t cells. peripheral blood lymphocytes (pbl) from all eight vaccines studied responded by proliferating after stimulation with hsv-2, hsv-1, and glycoprotein gb-1. the pbl of five of these eight vaccines proliferated following stimulation with gd-2, whereas stimulation with gd- ... | 1988 | 2846864 |
| on the control of immediate early (alpha) mrna survival in cells infected with herpes simplex virus. | the alpha or immediate early mrna of herpes simplex virus strain hsv-2(g) had a half-life of about 15 min if made in the absence of viral protein synthesis but was relatively stable if viral protein synthesis occurred, either freely or restricted by the presence of the proline analogue azetidine. in contrast, the alpha mrna of other strains of the virus is stable, even in the absence of protein synthesis. studies with recombinant viruses showed that the region of the viral dna between 0.58 and 0 ... | 1988 | 2846763 |
| paget's disease of the vulva: search for herpes simplex virus antigens and human papillomavirus antigen and dna. | specimens from three women with paget's disease of the vulva were tested for presence of herpes simplex virus type 2 (hsv2) antigens and human papillomavirus (hpv) antigen and dna. in one of the lesions, the hsv2-associated antigen icsp 34/35 was demonstrated. neither hpv antigens nor hpv dna were detected in the lesions. | 1988 | 2846413 |
| influence of sera from patients affected by neoplasia on some human macrophage functions. | it has been repeatedly reported that several functions of mononuclear cells are impaired in patients affected by neoplasia. moreover, inhibitory activity of serum and tumor extracts on macrophages have been described. in a previous study, we found a marked impairment of the intrinsic antiviral activity of macrophages derived from monocytes isolated from peripheral blood of patients with breast carcinoma or melanoma compared with that from blood of normal subjects. the aim of the present work was ... | 1988 | 2846174 |
| induction of cervical intraepithelial neoplasia in balb/c mice by uv-inactivated herpes simplex virus type 2. | 1988 | 2845906 | |
| an acyclovir-resistant strain of herpes simplex virus type 2 which is highly virulent for mice. | herpes simplex virus type 2 (hsv-2), strain ys-4 c-1, isolated by plaque cloning from a clinical isolate was found to be resistant to acyclovir (acv; acycloguanosine) in vitro. it was sensitive to phosphonoacetic acid and 9-beta-d-arabinofuranosyladenine. thymidine kinase (tk) activity of ys-4 c-1 was less than 1% of that of other strains from the same clinical source. however, thymidine plaque autoradiography showed that ys-4 c-1 was not completely deficient in tk activity. ys-4 c-1 showed high ... | 1988 | 2845890 |
| inhibition of terminal n- and o-glycosylation specific for herpesvirus-infected cells: mechanism of an inhibitor of sugar nucleotide transport across golgi membranes. | the nucleoside analog (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) inhibited the golgi-associated terminal glycosylation in herpes simplex virus type 1- and type 2-infected cells, specifically incorporation of galactose and sialic acid into n-linked oligosaccharides, and incorporation of sialic acid and, to a lesser extent, of galactose into o-linked oligo saccharides. this resulted in formation of viral glycoproteins with terminal glcnac and fuc in n-linked oligosaccharides and terminal o-linked ... | 1988 | 2845656 |
| modification of the genital herpes infection in a guinea pig model, by prior immunization with bovine herpes mammillitis virus. | bovine herpes mammillitis virus has been shown to partially protect guinea pigs against primary genital herpes simplex virus type 2 infections, further confirming the immunologic cross-reactivity of these two viruses. | 1988 | 2845232 |
| anti hsv-2 properties of amniotic fluid. | some antiviral properties of amniotic fluid (af) were studied. a close relationship between maternal blood and af antiviral antibodies was seen. substances other than antibodies which could have an effect on viruses were assumed to be present in af. using hsv2 as a model, we found a virus-neutralizing activity in 6 out of 7 specimens studied which does not appear to be related to antibodies. | 1988 | 2845231 |
| c3b receptor activity on transfected cells expressing glycoprotein c of herpes simplex virus types 1 and 2. | glycoprotein c from herpes simplex virus type 1 (gc-1 from hsv-1) acts as a receptor for the c3b fragment of the third component of complement on hsv-1-infected cell surfaces. direct binding assays with purified gc-1 and c3b demonstrate that other viral and cellular proteins are not required for this interaction. although c3b receptor activity is not expressed on hsv-2-infected cell surfaces, purified gc-2 specifically binds c3b in direct binding assays, suggesting that gc-1 and gc-2 are functio ... | 1988 | 2845122 |
| detection of herpes simplex virus type 1 in herpetic ocular diseases by dna-dna hybridization using a biotinylated dna probe. | a diagnostic hybridization assay for detecting herpes simplex virus type 1 (hsv-1) in ocular specimens was developed using cloned viral dna as a probe. this hybridization assay is based on visualizing a biotinylated probe that is hybridized to the target dna by a streptavidin/alkaline phosphatase system. the time required for performing this assay system is only two days. this assay system could detect a probe which had been hybridized to as little as 1 pg of homologous dna and did not cross-rea ... | 1988 | 2844977 |
| mutational analysis of the herpes simplex virus type 1 trans-inducing factor vmw65. | the herpes simplex virus type 1 (hsv-1) polypeptide vmw65 is a structural component of the virus particle and is also responsible for trans-induction of immediate early (ie) transcription. functional domains of this polypeptide were investigated by constructing a series of 10 plasmids each with a 12 bp insertion in the gene encoding vmw65. plasmids were analysed for their ability to stimulate ie transcription in short term transfection assays, and the altered vmw65 polypeptides were assayed for ... | 1988 | 2844968 |
| herpes simplex virus ii antigen in adenocarcinoma of the uterine cervix. | 1988 | 2844663 | |
| infection of human thymic epithelial cells by human cytomegalovirus and other viruses: effect on secretion of interleukin 1-like activity. | cultures of human thymic epithelial (te) cells are able to produce a interleukin 1 (il-1) like activity. this il-1 activity can be detected either using mouse thymocytes in a traditional il-1 assay, or using thymic lymphocytes obtained from cases of pediatric cardio-vascular surgery. production of il-1 activity by te cells was found to be maximal between 3 and 4 weeks after culture initiation. human thymocytes worked best as targets in an il-1 assay, when these cells were derived from donors you ... | 1988 | 2844452 |
| multicentric intraepithelial neoplasia involving the vulva. clinical features and association with human papillomavirus and herpes simplex virus. | sixteen of 46 patients (35%) with grade 3 vulvar intraepithelial neoplasia (vin 3) were found to have an additional site of lower genital tract squamous cell neoplasia, primarily in the cervix. the frequency of multicentricity decreased significantly with age. in addition, patients with multicentric disease (involving the vagina and/or cervix in addition to the vulva) had a significantly higher frequency of multifocal disease involving the vulva (involving more than one location on the vulva) an ... | 1988 | 2844383 |
| effectiveness of a procedure for disinfecting the vaginal photoplethysmograph contaminated with herpes simplex virus type 2. | to test the effectiveness of a disinfecting procedure involving 2% glutaraldehyde, a vaginal photoplethysmograph was contaminated with a known amount of herpes simplex type 2 (hsv-2). the vaginal photoplethysmograph was then put through the disinfection procedure. two virus solutions were tested, one designed to approximate the concentration found in a naturally occurring infection (low inoculum), the other with 100 times more virus (high inoculum). varying lengths of exposure to glutaraldehyde ... | 1988 | 2844137 |
| placebo-controlled study with subunit herpes simplex virus vaccine in subjects suffering from frequent herpetic recurrences. | the safety and efficacy of a subunit herpes simplex virus (hsv) type 1 vaccine were tested in a small-scale double-blind trial carried out in a group of 42 volunteers suffering from frequent recurrences of herpetic lesions. the patients were paired according to sex, age, type of virus isolated, previous history of the disease and some non-specific immunological markers. one member of each pair received repeated doses of hsv vaccine, the other a placebo. clinical reactions were mild. antibody res ... | 1988 | 2844031 |
| inducible expression of herpes simplex virus type 2 glycoprotein gene gg-2 in a mammalian cell line. | the gg-2 glycoprotein gene of herpes simplex virus type 2 (hsv-2) was cloned into the mammalian expression vector pmsg under the control of the inducible mouse mammary tumor virus promoter. transfection of this cloned gg-2 construct into nih 3t3 cells resulted in the stable expression of gg-2 upon induction with dexamethasone. in addition, the 104,000-molecular-weight (104k) and 72k gg-2 precursors as well as the 34k secreted component were generated in the transformed cells. the synthesis of gg ... | 1988 | 2843667 |
| increased efficacy of phosphonoformate and phosphonoacetate inhibition of herpes simplex virus type 2 replication by encapsulation in liposomes. | phosphonoformate and phosphonoacetate encapsulated in liposomes have substantially greater activity against herpes simplex virus type 2 in vero cell tissue culture than the nonencapsulated compounds at the same dose. encapsulation of phosphonoformate in liposomes resulted in a 30-fold increase of the antiviral effect with no increase in cytotoxicity measured by inhibition of thymidine incorporation into normal vero cells. thus, the selectivity of the liposomal drug increased 27-fold compared wit ... | 1988 | 2843083 |
| evaluation of macrophage antiviral activity in patients affected by neoplasia. | the intrinsic antiviral activity of macrophages has been studied in healthy donors and in patients affected by breast cancer and melanoma. in vitro differentiated macrophages from blood-derived monocytes were infected with measles virus, herpes simplex virus type 2 and adenovirus 17. the challenge was carried out with different multiplicities of infection and the synthesis of virus was tested by evaluating the single cycle growth curve in 24 h. the results obtained show that the restriction of v ... | 1988 | 2842553 |
| phosphonoformate and phosphonoacetate derivatives of 5-substituted 2'-deoxyuridines: synthesis and antiviral activity. | the synthesis of potential "combined prodrugs" wherein phosphonoformate or phosphonoacetate was attached to the 5'-position of 2'-deoxyuridine, 2'-deoxythymidine, 5-iodo-2'-deoxyuridine (idu), 5-(2-chloroethyl)-2'-deoxyuridine (cedu), or 5-(2-bromovinyl)-2'-deoxyuridine (bvdu) or to the 3'-position of cedu is described. the antiviral activities of these derivatives and of reference compounds were compared in vero, hep-2, and primary rabbit kidney cells against herpes simplex virus types 1 and 2 ... | 1988 | 2842506 |
| history of recurrent mucocutaneous herpes correlates with relatively low interferon production by herpes simplex virus-exposed cultured monocytes. | monocytes separated from blood specimens of healthy voluntary blood donors were infected with herpes simplex virus (hsv) 1 or 2. the amount of interferon produced by the hsv-exposed monocytes was found to be donor-specific and was similar whether tested with hsv 1 or hsv 2. the interferon levels in repeated blood specimens from a given donor were relatively stable within an observation period of a few months. the survey was expanded by testing 27 persons with history of recurrent herpes disease ... | 1988 | 2842448 |
| in vivo behavior of genetically engineered herpes simplex viruses r7017 and r7020: construction and evaluation in rodents. | the herpes simplex virus (hsv) recombinant r7017 was constructed from hsv-1 (strain f) by deleting a portion of the thymidine kinase (tk) gene and by replacing the sequences representing the internal inverted repeats and adjacent genes in the l component with a fragment of the hsv-2 genome encoding the glycoproteins g, d, i, and a portion of e. in addition, the r7020 recombinant contains an hsv-1 dna fragment encoding the tk gene fused to the alpha 4 gene promoter. the results of studies in mice ... | 1988 | 2842408 |
| a cellular protein related to heat-shock protein 90 accumulates during herpes simplex virus infection and is overexpressed in transformed cells. | a monoclonal antibody produced from mice immunized with hsv-infected cell dna binding proteins reacts with two cell-encoded polypeptides, p90 and p40, synthesized constitutively by many different cell types and expressed at high levels in transformed cells. in uninfected cells heat shock induces a cytoplasmic accumulation of p90 and in agreement with this we show that p90 is related to hsp90. during hsv-2 lytic productive infection, p90 accumulates to very high levels, such accumulation being de ... | 1988 | 2842174 |
| specific induction of cellular gene transcription in herpes simplex virus type 2-transformed cells. | in spite of much work, the mechanism of oncogenic transformation by herpes simplex virus (hsv) is as yet unknown. it has been proposed that hsv type 2 (hsv-2) can transform cells by a 'hit and run' mechanism. in the past we have demonstrated that several polypeptides can be immunoprecipitated from hsv-2-transformed cells, but not from control cells or adenovirus-transformed cells, by rabbit hyperimmune sera to hsv-2. it is possible that the expression of these proteins might be the result of act ... | 1988 | 2841413 |
| effect of macrophage activation on resistance of mouse peritoneal macrophages to infection with herpes simplex virus types 1 and 2. | to define the effect of heterogeneity of murine peritoneal macrophages (m phi) on intrinsic resistance to herpes simplex virus (hsv) infection, several m phi populations were characterized for their response to infection with hsv type 1 (hsv-1) and hsv-2. steady-state resident m phi (res m phi) were compared in parallel with m phi activated with corynebacterium parvum (now designated propionibacterium acnes) (cp m phi) and thioglycollate-elicited inflammatory m phi (tg m phi). res m phi were com ... | 1988 | 2841412 |
| detection of herpes simplex virus type 2 dna in uterine cervix lesions using cloned bgl ii n fragment of hsv-2 dna as a probe. | 1988 | 2840390 | |
| methyl gallate, methyl-3,4,5-trihydoxybenzoate, is a potent and highly specific inhibitor of herpes simplex virus in vitro. ii. antiviral activity of methyl gallate and its derivatives. | methyl gallate (mg), methyl-3,4,5-trihydroxybenzoate, was highly active against herpes viruses as determined by plaque reduction assay. herpes simplex virus type 2, ms strain, was sensitive to mg at a mean 50% inhibitory concentration (ic50) of 0.224 micrograms/ml in monkey kidney cells. mg was specific for herpes viruses with the relative sensitivity hsv-2 greater than hsv-1 greater than cmv. two rna viruses tested were significantly less sensitive to mg. the structural components of mg which m ... | 1988 | 2840133 |
| methyl gallate, methyl-3,4,5-trihydroxybenzoate, is a potent and highly specific inhibitor of herpes simplex virus in vitro. i. purification and characterization of methyl gallate from sapium sebiferum. | a potent anti-herpetic compound was identified and purified to homogeneity from the leaves of sapium sebiferum by plaque reduction assay using herpes simplex virus type 2. the chemical structure of the purified compound was determined by mass spectroscopy and proton and carbon-13 nuclear magnetic resonance as methyl gallate, methyl-3,4,5-trihydroxybenzoate. this is the first demonstration that methyl gallate is a potent anti-herpetic compound in vitro, present in high concentration in the leaves ... | 1988 | 2840132 |
| changing patterns of genital herpes simplex virus infections. | herpes simplex virus type 2 is considered by many venereologists as the predominant serotype among those patients suffering from genital herpes. however, identification of genital isolates obtained from 16 through 20, and 31 through 40 year old individuals among our female patient population shows a predominance of hsv-1 infections. these data suggest that the "above the waist"-"below the waist" epidemiologic patterns commonly ascribed to reflect a prevalence of hsv-1 and hsv-2 infections respec ... | 1988 | 2839940 |
| purification of the herpes simplex virus type 1 65-kilodalton dna-binding protein: properties of the protein and evidence of its association with the virus-encoded dna polymerase. | using a combination of conventional column chromatography and velocity sedimentation, we have purified the 65-kilodalton dna-binding protein (65kdbp) encoded by herpes simplex virus (hsv) greater than 625-fold. the hsv type 1 (hsv-1)-encoded dna polymerase (pol) cofractionated with 65kdbp through deae-sephacel, blue sepharose, and mono q columns and was only separated from 65kdbp by sedimentation through a glycerol gradient. immunoaffinity columns containing monoclonal antibody (mab) 6898 immuno ... | 1988 | 2839706 |
| inhibition of herpes simplex virus type 2 replication in vitro by 1-n-pentadecanoyl-3''-n-trifluoroacetyl kanamycin a. | the in vitro antiviral activity as well as the mechanism of action of a new antiviral agent, a kanamycin analogue, 1-n-pentadecanoyl-3''-n-trifluoroacetyl kanamycin a (ptka) against herpes simplex virus type 2 (hsv-2) was investigated. the drug showed excellent antiviral action with negligible cytotoxic effect on the culture cells. based on plaque reduction assays the 50% inhibitory dose (id50) of the drug was 1 microgram/ml, and at 20 micrograms/ml plaque formation was totally suppressed. the c ... | 1988 | 2839107 |
| effect of herpes simplex virus type 2 infection on mitochondrial gene expression. | a cdna clone derived from the mitochondrial cytochrome oxidase i gene has been used to show that the level of mitochondrially encoded rna species declines during herpes simplex virus type 2 infection in a manner similar to that for rna species derived from nuclear genes. in contrast to the situation for nuclear genes, however, no change in the transcription rate of the mitochondrial genome during infection was detected, indicating that post-transcriptional processes alone are responsible for the ... | 1988 | 2838578 |
| interaction of polylysine with the cellular receptor for herpes simplex virus type 1. | we earlier reported that neomycin blocked reversibly the binding of herpes simplex virus type 1 (hsv-1) to the receptor of bhk cells, while the binding of hsv-2 to the receptor was unaffected. we could not determine whether the effect was on the virus particle, the receptor, or both. we have now tested several other cationic substances, and report that polylysine (and polyarginine) block the binding of hsv-1 to the receptor by interfering with the cellular receptor function; higher molecular wei ... | 1988 | 2838567 |
| regulation of viral and cellular genes in a human neuroblastoma cell line latently infected with herpes simplex virus type 2. | a latent state of the herpes simplex virus type 2 genome was established in a human neuroblastoma cell line (sms-kcnr) to initiate studies on the mechanism by which host cells interact and regulate latent viral genes. to establish viral latency, it was necessary to prevent virus replication by briefly exposing the infected cells to antiherpetic acycloguanosine (20 microm) and human interferon (120 u/ml). subsequently however, these cells could be propagated without any antiherpetic agents and al ... | 1988 | 2838126 |
| [prevention of lymphatic and hematologic spreading as a pathogenic condition for neural persistence of herpes simplex virus]. | in the experimental model of genital herpes simplex virus (hsv) infection of the mouse--in spite of abundant virus replication on the mucous membranes--no infectious virus can be isolated from the inflamed and swollen draining lymph nodes (dln), contrary to the positive results in the lumbosacral nerves and their associated ganglia. attempts to reactivate an abortive infection possibly established in lymph node or spleen cells by stimulation with phytohemagglutinin or lipopolysaccharide rendered ... | 1988 | 2837877 |
| hypomethylation of host cell dna synthesized after infection or transformation of cells by herpes simplex virus. | infection of rat embryo cells with herpes simplex virus type 2 caused undermethylation of host cell dna synthesized during infection. dna made prior to infection was not demethylated, but some of its degradation products, including methyl dcmp, were incorporated into viral dna. the use of mutant virus showed that some viral dna synthesis appears to be required for the inhibition of methylation. inhibition of methylation cannot be explained by an absence of dna methyltransferase as the activity o ... | 1988 | 2837642 |