Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [characterisation of a phenotypic monophasic variant belonging to salmonella enterica subsp. enterica serovar typhimurium from wild birds and its possible transmission to cats and humans]. | in the last two years the national salmonella reference laboratory (nrl-salm) received an accumulating number of salmonellae with sero-formula 4,12:-:1,2 isolated from perished wild birds, particularly siskins. within these strains flagellar antigen of the first phase was phenotypically not detectable. by pcr a fragment could be amplified coding specifically for the h:i-flagellar antigen. consequently, this is a phenotypically monophasic variant of salmonella enterica subsp. enterica serovar typ ... | 2009 | 19517930 |
| an outbreak of salmonella enterica serotype litchfield infection in australia linked to consumption of contaminated papaya. | an outbreak of 26 cases of salmonella litchfield infection occurred in the states of western australia and queensland between october 2006 and january 2007. a case-control study was conducted with 12 cases and 24 controls, and a significant association was found between illness and consumption of papaya (odds ratio, 32.8; 95% confidence interval, 2.71 to 883.5). papaya samples were collected from 26 stores in western australia, and 9 of 38 samples were contaminated with salmonella litchfield. th ... | 2009 | 19517740 |
| characterization of planktonic and biofilm communities of day-of-hatch chicks cecal microflora and their resistance to salmonella colonization. | recent concerns about the use of antimicrobials in food animals have increased interest in the microbial ecology and biofilms within their gastrointestinal tract. this work used a continuous-flow chemostat system to model the microbial community within the ceca from day-of-hatch chicks and its ability to resist colonization by salmonella enterica serovar typhimurium. we characterized the biofilm and planktonic communities from five cultures by using automated ribotyping. eight species from six d ... | 2009 | 19517721 |
| chlorine dioxide gas from an aqueous solution: reduction of salmonella in wounds on tomato fruit and movement to sinks in a treatment chamber. | chlorine dioxide (clo2) off-gassed from an aqueous solution and reacted incrementally with potassium iodide solutions (sinks). after 30 min, 45% of the initial dose was detected as chlorite ion in the sink, whereas 35% of the initial dose was still in the source. aqueous solutions of clo2 can be used as a source of clo2 gas in various laboratory experiments involving treatment of fruits or vegetables. movement from source to sink is continuous, which precludes the development of large headspace ... | 2009 | 19517720 |
| priming of salmonella enterica serovar typhi-specific cd8(+) t cells by suicide dendritic cell cross-presentation in humans. | the emergence of antibiotic-resistant strains of salmonella enterica serovar typhi (s. typhi), the etiologic agent of typhoid fever, has aggravated an already important public health problem and added new urgency to the development of more effective typhoid vaccines. to this end it is critical to better understand the induction of immunity to s. typhi. cd8(+) t cells are likely to play an important role in host defense against s. typhi by several effector mechanisms, including killing of infecte ... | 2009 | 19517022 |
| kinetic and functional analysis of l-threonine kinase, the pdux enzyme of salmonella enterica. | the pdux enzyme of salmonella enterica is an l-threonine kinase used for the de novo synthesis of coenzyme b(12) and the assimilation of cobyric acid. pdux with an n-terminal histidine tag (his(8)-pdux) was produced in escherichiacoli and purified. the recombinant enzyme was soluble and active. kinetic analysis indicated a steady-state ordered bi bi complex mechanism in which atp is the first substrate to bind. based on a multiple sequence alignment of pdux homologues and other ghmp (galactokina ... | 2009 | 19509296 |
| h-ns modulates multidrug resistance of salmonella enterica serovar typhimurium by repressing multidrug efflux genes acref. | screening of salmonella mutants for the ability to increase beta-lactam resistance has led to the identification of a mutation in hns, which codes for the histone-like nucleoid structuring protein (h-ns). in this study, we report that h-ns modulates multidrug resistance through repression of the genes that encode the acref multidrug efflux pump in salmonella enterica serovar typhimurium. | 2009 | 19506059 |
| mesenteric lymph nodes confine dendritic cell-mediated dissemination of salmonella enterica serovar typhimurium and limit systemic disease in mice. | in humans with typhoid fever or in mouse strains susceptible to salmonella enterica serovar typhimurium (s. typhimurium) infection, bacteria gain access to extraintestinal tissues, causing severe systemic disease. here we show that in the gut-draining mesenteric lymph nodes (mln), the majority of s. typhimurium-carrying cells show dendritic-cell (dc) morphology and express the dc marker cd11c, indicating that s. typhimurium bacteria are transported to the mln by migratory dcs. in vivo flt-3l-ind ... | 2009 | 19506012 |
| macrophage-specific overexpression of antimicrobial peptide pr-39 inhibits intracellular growth of salmonella enterica serovar typhimurium in macrophage cells. | although purified and synthesised pr-39 shows potent antibacterial effects in vitro, its ability to kill intracellular bacteria in macrophages, which are a major cause of refractory intracellular infection, has not yet been demonstrated. both to enhance its antimicrobial potential and to reduce systemic side effects, it would be desirable to deliver pr-39 into macrophage cells and to limit its activation to the site of infection. to address this issue, pr-39 dna was inserted into the eukaryotic ... | 2009 | 19505806 |
| escherichia coli o123 o antigen genes and polysaccharide structure are conserved in some salmonella enterica serogroups. | the serotyping of o and h antigens is an important first step in the characterization of salmonella enterica. however, serotyping has become increasingly technically demanding and expensive to perform. we have therefore sequenced additional s. enterica o antigen gene clusters to provide information for the development of dna-based serotyping methods. three s. enterica isolates had o antigen gene clusters with homology to the escherichia coli o123 o antigen region. o antigen clusters from two ser ... | 2009 | 19502376 |
| stress-induced prophage dna replication in salmonella enterica serovar typhimurium. | salmonella typhimurium, a foodborne pathogen, is the cause of new outbreaks every year. the virulence of new pathogens is determined by their virulence genes, many of them carried on transferable elements, such as prophages. in bacteria harboring multiple prophages such as salmonella, the reassortment of these genes plays a major role in the emergence of new pathogens and consequently new epidemics. this gene transfer depends on prophage induction and the initiation of the phage lytic cycle. in ... | 2009 | 19501196 |
| slc11a1 limits intracellular growth of salmonella enterica sv. typhimurium by promoting macrophage immune effector functions and impairing bacterial iron acquisition. | the natural resistance-associated macrophage protein 1, slc11a1, is a phagolysosomal transporter for protons and divalent ions including iron that confers host protection against diverse intracellular pathogens including salmonella. we investigated and compared the regulation of iron homeostasis and immune function in raw264.7 murine phagocytes stably transfected with non-functional slc11a1 and functional slc11a1 controls in response to an infection with salmonella enterica serovar typhimurium. ... | 2009 | 19500110 |
| mapping markers linked to porcine salmonellosis susceptibility. | the goal of this study was to identify pig chromosomal regions associated with susceptibility to salmonellosis. genomic dna from pig reference populations with differences in susceptibility to salmonella enterica serovar choleraesuis as quantified by spleen and liver bacterial colonization at day 7 post-infection (dpi; van diemen et al. 2002) was used. these samples belonged to the offspring of a sire thought to be heterozygous for genes involved in susceptibility to salmonellosis. amplified fra ... | 2009 | 19496771 |
| lac repressor is an antivirulence factor of salmonella enterica: its role in the evolution of virulence in salmonella. | the genus salmonella includes many pathogens of great medical and veterinary importance. bacteria belonging to this genus are very closely related to those belonging to the genus escherichia. laczya operon and laci are present in escherichia coli, but not in salmonella enterica. it has been proposed that salmonella has lost laczya operon and laci during evolution. in this study, we have investigated the physiological and evolutionary significance of the absence of laci in salmonella enterica. us ... | 2009 | 19495420 |
| membrane-protein structure: piercing insights. | 2009 | 19494904 | |
| correlations between carbon metabolism and virulence in bacteria. | bacteria have developed several mechanisms which allow the preferred utilization of the most efficiently metabolizable carbohydrates when these organisms are exposed to a mixture of carbon sources. interestingly, the same or similar mechanisms are used by some pathogens to control various steps of their infection process. the efficient metabolism of a carbon source might serve as signal for proper fitness. alternatively, the presence of a specific carbon source might indicate to bacterial cells ... | 2009 | 19494580 |
| 10-undecanhydroxamic acid, a hydroxamate derivative of the undecanoic acid, has strong antimicrobial activity through a mechanism that limits iron availability. | undecanoic acid (uda) is a fatty acid with significant antimycotic activity. in this work we have synthesized 10-undecanhydroxamic acid, a hydroxamate derivative of the uda, and tested its antimicrobial activity on different microorganisms. our results demonstrate that this compound has higher efficacy than uda against a variety of fungi and bacteria. analysis of the intracellular concentration of protein involved in iron transport in salmonella enterica serovar typhimurium suggests that its ant ... | 2009 | 19493009 |
| deletion of the gene encoding the glycolytic enzyme triosephosphate isomerase (tpi) alters morphology of salmonella enterica serovar typhimurium and decreases fitness in mice. | the glycolytic enzyme triosephosphate isomerase (tpi) (ec 5.3.1.1) plays a key role in central carbon metabolism yet few studies have characterized isogenic bacterial mutants lacking this enzyme and none have examined its role in the in vivo fitness of a bacterial pathogen. here we have deleted tpia in salmonella enterica serovar typhimurium and found that the mutant had an altered morphology, displaying an elongated shape compared with the wild type. in a mouse model of typhoid fever the tpia m ... | 2009 | 19493007 |
| competition among isolates of salmonella enterica ssp. enterica serovar typhimurium: role of prophage/phage in archived cultures. | previously, we reported extensive diversity among survivors of salmonella enterica ssp. enterica serovar typhimurium that were stored for four decades in sealed agar stabs. thus raising the question: was there selection for greater fitness among eventual survivors? to address this, we cocultured archived lt2 survivors with nonarchived (parental) lt2 strains in competition experiments. selected archived strains outgrew a nonarchived lt2 sequenced strain. although we initially assumed this was the ... | 2009 | 19493006 |
| enhanced vaccine antigen delivery by salmonella using antibiotic-free operator-repressor titration-based plasmid stabilisation compared to chromosomal integration. | live attenuated bacteria provide the potential to replace traditional needle-based vaccination with an orally administered vaccine. the heterologous antigen gene is usually transformed as a multi-copy plasmid into the bacterial cell, but plasmids in live bacterial vaccine strains are often unstable, so an alternative approach is to integrate the single-copy antigen gene into the bacterial chromosome. we report a comparison between the chromosomally integrated and the plasmid-borne bacillus anthr ... | 2009 | 19490834 |
| redundancy in the requirement for the glycolytic enzymes phosphofructokinase (pfk) 1 and 2 in the in vivo fitness of salmonella enterica serovar typhimurium. | to assess the role of the glycolytic enzyme phosphofructokinase (pfk) in the in vivo fitness of the pathogen salmonella enterica serovar typhimurium (s. typhimurium) we have generated single and double gene deletion mutants of the two known isoforms of this enzyme, pfka and pfkb. in a mouse model of typhoid fever, bacterial counts in the spleen and liver were similar between wild type and single pfka and pfkb mutant-infected mice. however, a double pfkab mutant was significantly attenuated for g ... | 2009 | 19486643 |
| long-term survival of salmonella enterica serovar typhimurium reveals an infectious state that is underrepresented on laboratory media containing bile salts. | cells in desiccated salmonella enterica serovar typhimurium rdar (red, dry, and rough) morphotype colonies were examined for culturability and infectivity after 30 months. culturability decreased only 10-fold; however, cells were underrepresented on salmonella selective media containing bile salts. these cells were mildly attenuated compared to the infectivity of freshly grown cells but still able to cause systemic infections in mice. | 2009 | 19482950 |
| immunisation of the equine uterus against streptococcus equi subspecies zooepidemicus using an intranasal attenuated salmonella vector. | attenuated salmonella enterica serovar typhimurium mgn707, expressing the szp protective protein of the mb9 serovar of streptococcus equi subspecies zooepidemicus (szp-mb9) was tested for its safety and efficacy as a nebulised intranasal vaccine against streptococcal uterine infections in mares. in a preliminary study, vaccinated mares (n=5) displayed serum, nasal and uterine responses (p<0.05) to s. typhimurium lipopolysaccharide (st-lps). subsequently, vaccinated mares (expressor group, n=7), ... | 2010 | 19482493 |
| [detection of bla ctx-m-14 and aac(3)-ii genes in salmonella enterica serotype kedougou in algeria]. | 2009 | 19482452 | |
| gold screen-printed-based impedimetric immunobiosensors for direct and sensitive escherichia coli quantisation. | label-free electrochemical impedance immunosensors for the detection and quantification of escherichia coli (e. coli) using self-assembled monolayers (sams)-modified gold screen-printed electrodes (auspes) were developed. two different immunosensor configurations were tested and compared. in the first one, the immunosensing design was based on the covalent immobilization of anti-e. coli at auspes using the homobifunctional cross-linker 3,3'-dithiobis[sulfosuccinimidylpropionate] (dtssp). the oth ... | 2009 | 19481924 |
| the base excision repair system of salmonella enterica serovar typhimurium counteracts dna damage by host nitric oxide. | intracellular pathogens must withstand nitric oxide (no.) generated by host phagocytes. salmonella enterica serovar typhimurium interferes with intracellular trafficking of inducible nitric oxide synthase (inos) and possesses multiple systems to detoxify no.. consequently, the level of no. stress encountered by s. typhimurium during infection in vivo has been unknown. the base excision repair (ber) system recognizes and repairs damaged dna bases including cytosine and guanine residues modified b ... | 2009 | 19478870 |
| the effect of methylation on some biological parameters in salmonella enterica serovar typhimurium. | cell growth is tightly coupled to dna replication and its methylation [proc natl acad sci u s a 93 (1996) 12206-12211]. in a culture medium, growing of salmonella typhimurium (s. typhimurium) mutant cells (dam(-)) decreased (2.5 fold) relative to the wild type strain (dam(+)). in this study, we show that the reason for this growth deficiency is due to the dna methylation. the absence of a dam methyltransferase protein results in poor growth efficiency and disturbs the synchrony of replication in ... | 2009 | 19477083 |
| extended-spectrum {beta}-lactamases and ampc {beta}-lactamases in ceftiofur-resistant salmonella enterica isolates from food and livestock obtained in germany during 2003-07. | detection and characterization of extended-spectrum beta-lactamases (esbls) and ampc-encoding genes was conducted in german salmonella isolated from different sources from 2003 to 2007. | 2009 | 19474065 |
| outer membrane protein stm3031 (ail/ompx-like protein) plays a key role in the ceftriaxone resistance of salmonella enterica serovar typhimurium. | previously, the putative outer membrane protein stm3031 has been correlated with ceftriaxone resistance in salmonella enterica serovar typhimurium. in this study, this protein was almost undetectable in the ceftriaxone-susceptible strain 01-4, but its levels were increased in 01-4 isogenic strains for which mics were higher. the stm3031 gene deletion mutant, r200(deltastm3031), was generated and showed >64-fold lower ceftriaxone resistance than r200, supporting a key role for stm3031 in ceftriax ... | 2009 | 19470505 |
| insights into the evolution of sialic acid catabolism among bacteria. | sialic acids comprise a family of nine-carbon amino sugars that are prevalent in mucus rich environments. sialic acids from the human host are used by a number of pathogens as an energy source. here we explore the evolution of the genes involved in the catabolism of sialic acid. | 2009 | 19470179 |
| relationships between multidrug-resistant salmonella enterica serovar schwarzengrund and both broiler chickens and retail chicken meats in japan. | we examined 29 isolates of salmonella enterica subspecies enterica serovar schwarzengrund from broiler chickens (n=19) and retail chicken meats (n=10) in japan for antimicrobial susceptibility and pulsed-field gel electrophoresis (pfge) profiling. all isolates exhibited resistance to both bicozamycin and sulfadimethoxine (minimum inhibitory concentration of both antimicrobial agents: >512 microg/ml). nalidixic acid resistance was found in only one broiler chicken isolate. pfge analysis showed th ... | 2009 | 19468180 |
| the single substitution i259t, conserved in the plasminogen activator pla of pandemic yersinia pestis branches, enhances fibrinolytic activity. | the outer membrane plasminogen activator pla of yersinia pestis is a central virulence factor in plague. the primary structure of the pla beta-barrel is conserved in y. pestis biovars antiqua, medievalis, and orientalis, which are associated with pandemics of plague. the pla molecule of the ancestral y. pestis lineages microtus and angola carries the single amino acid change t259i located in surface loop 5 of the beta-barrel. recombinant y. pestis kim d34 or escherichia coli xl1 expressing pla t ... | 2009 | 19465664 |
| evaluation of salmonella live vaccines with chromosomal expression cassettes for translocated fusion proteins. | salmonella enterica is a versatile live carrier for the presentation of recombinant vaccine antigens. fusion proteins of a type iii secretion system effector and heterologous vaccine antigens can be translocated by live attenuated salmonella strains and mediate protective immunity against infections. here we investigated the use expression cassettes for translocated fusion protein consisting of effector ssef and antigens of listeria monocytogenes after stable integration into the salmonella chro ... | 2009 | 19464562 |
| flow cytometric analysis of salmonella enterica serotype typhimurium inactivated with supercritical carbon dioxide. | non-thermal processes for the effective sterilization and inactivation of microorganisms are currently receiving a great deal of attention in food, pharmaceutical and other relevant industries. supercritical carbon dioxide (sc-co(2)) treatment is an alternative method of microbial inactivation that can be safely used in foods and bioactive materials at relatively low temperatures. however, to date, the inactivation of microbial cells by treatment with sc-co(2) has only been evaluated using a con ... | 2009 | 19463861 |
| structure of o-antigen and functional characterization of o-antigen gene cluster of salmonella enterica o47 containing ribitol phosphate and 2-acetimidoylamino-2,6-dideoxy-l-galactose. | an o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of salmonella enterica o47 and studied by sugar analysis along with one- and two-dimensional 1h- and 13c-nmr spectroscopy. the following structure of the linear ribitol phosphate-containing repeating unit of the o-polysaccharide was established: -->2)-d-ribitol-5-p-(o-->6)-alpha-d-galp-(1-->3)-alpha-l-fucpnam-(1-->3)-beta-d-glcpnac-(1-->, where fucnam stands for 2-acetimidoylamino-2,6-dideoxy-l-galactose. about 1 ... | 2009 | 19463095 |
| soxs regulates the expression of the salmonella enterica serovar typhimurium ompw gene. | ompw of salmonella enterica serovar typhimurium has been described as a minor porin involved in osmoregulation, and is also affected by environmental conditions. biochemical and genetic evidence from our laboratory indicates that ompw is involved in efflux of and resistance towards paraquat (pq), and its expression has been shown to be activated in response to oxidative stress. in this study we have explored ompw expression in response to pq. primer extension and transcriptional fusions showed t ... | 2009 | 19460824 |
| molecular typing methodologies for microbial source tracking and epidemiological investigations of gram-negative bacterial foodborne pathogens. | gram-negative bacterial foodborne pathogens are a worldwide cause of morbidity and mortality. the ability to carry out epidemiological investigations to determine the primary sources of bacterial contamination is important to improve public health. multiple methods are available for bacterial source tracking and to determine the distribution of pathogens isolated from sick patients. the molecular based typing methods available fall into three general categories: those based on restriction analys ... | 2009 | 19460308 |
| a yeast-based genetic screen for identification of pathogenic salmonella proteins. | salmonella uses type iii secretion systems (ttss) to deliver pathogenic proteins into the host cells. these translocated effectors induce bacterial internalization and intracellular proliferation by targeting important cellular processes that are conserved among eukaryotes. here, we assessed the feasibility of performing a genetic screen in yeast to identify novel salmonella effectors, by searching for genes that produce toxicity when expressed in this model system. we identified several known t ... | 2009 | 19459960 |
| antimicrobial activity of enterococcus faecium ef 55 against salmonella enteritidis in chicks. | the protective effect of enterococcus faecium ef 55 against salmonella enterica serovar enteritidis phage type 4 (se pt4) was studied in 1-day-old chicks. the ef 55 strain (isolated and characterised by the authors earlier) was applied daily (1.10(9) cfu/0.2 ml pbs) for 7 days. oral inoculation of the se pt4 strain was performed on day 8 in a single dose of 5.10(8) cfu/0.2 ml pbs. the experiment lasted for 21 days. samples were collected on day 1 of the experiment to verify the absence of salmon ... | 2009 | 19457770 |
| survival of enteric bacteria and coliphage ms2 in pure human urine. | the survival of escherichia coli, salmonella enterica serovar typhimurium, enterococcus faecalis and coliphage ms2 was studied in stored, fresh and diluted (1 : 1) human urine at 15 and 30 degrees c. | 2009 | 19457041 |
| lipocalin-2 resistance confers an advantage to salmonella enterica serotype typhimurium for growth and survival in the inflamed intestine. | in response to enteric pathogens, the inflamed intestine produces antimicrobial proteins, a process mediated by the cytokines il-17 and il-22. salmonella enterica serotype typhimurium thrives in the inflamed intestinal environment, suggesting that the pathogen is resistant to antimicrobials it encounters in the intestinal lumen. however, the identity of these antimicrobials and corresponding bacterial resistance mechanisms remain unknown. here, we report that enteric infection of rhesus macaques ... | 2009 | 19454351 |
| a precious metal heist. | nearly all bacterial pathogens require iron to successfully infect their vertebrate hosts. the host molecule lipocalin-2 exploits this by sequestering bacterial siderophores as a mechanism of protection against infection. raffatellu et al. (2009) show that salmonella enterica serotype typhimurium circumvents this through the production of the modified siderophore salmochelin. | 2009 | 19454345 |
| host-specificity of salmonella enterica serovar gallinarum: insights from comparative genomics. | in this study, we have identified the possible genetic factors responsible for fowl-adaptation of salmonella entericaserovar gallinarum (s. gallinarum). by comparing the genes related to salmonella pathogenicity islands (spi) of s. gallinarum with those of salmonella entericaserovar enteritidis (s. enteritidis) we have identified twenty-four positively selected genes. our results suggest that the genes encoding the structural components of spi-2 encoded type three secretion apparatus (ttss) and ... | 2009 | 19454277 |
| nitric oxide-induced membrane tubulovesicular extensions (cytonemes) of human neutrophils catch and hold salmonella enterica serovar typhimurium at a distance from the cell surface. | nitric oxide (no) plays an important role in host defense against bacterial infections such as salmonellosis. no and 4-bromophenacyl bromide (bpb) induce the formation of long tubulovesicular extensions (tve, cytonemes, membrane tethers) from human neutrophils. these tve serve as cellular sensory and adhesive organelles. in the present study, we demonstrated that in the presence of the no donor, diethylamine nonoate or bpb human neutrophils bound and aggregated salmonella enterica serovar typhim ... | 2009 | 19453754 |
| first report of plasmid-mediated quinolone resistance determinant qnrs1 in an escherichia coli strain of animal origin in italy. | a qnrs1-positive strain of escherichia coli was detected among 73 poultry isolates showing ciprofloxacin mics of > or =0.125 microg/ml. the qnrs1 gene was associated with a tn3-like transposon, as previously described to occur in a salmonella enterica serovar infantis strain of animal origin, but the plasmid scaffold carrying this element resembled that of a plasmid previously identified in salmonella enterica serovar dublin. these elements suggest genetic exchanges among salmonella and e. coli ... | 2009 | 19451298 |
| the capsule-encoding viab locus reduces intestinal inflammation by a salmonella pathogenicity island 1-independent mechanism. | salmonella enterica serotype typhimurium elicits acute neutrophil influx in the human intestinal mucosa within 1 or 2 days after infection, resulting in inflammatory diarrhea. in contrast, no overt symptoms are observed within the first 1 or 2 weeks after infection with s. enterica serotype typhi. here we show that introduction of the capsule-encoding viab locus of serotype typhi reduced the ability of serotype typhimurium to elicit acute intestinal inflammation in a streptomycin-pretreated mous ... | 2009 | 19451244 |
| salmonella enterica serovar typhimurium-induced internalization and il-8 expression in hela cells does not have a direct relationship with intracellular ca(2+) levels. | the invasion-associated type iii secretion system (t3ss-1) of s. typhimurium is required to initiate and sustain an acute inflammatory response in the intestine. we investigated the relationship of s. typhimurium t3ss-1-induced il-8 expression and invasion with intracellular ca(2+) mobilization in hela cells. compared to the sipasopabde2 mutant, strains carrying a mutation in sipa, or mutations in sopabde2 induced higher levels of il-8 and greater bacterial internalization despite the fact that ... | 2009 | 19450704 |
| effect of analgesics and their derivatives on antibiotic resistance of environmental microbes. | this research is a preliminary study conducted to determine the effects of aspirin (acetyl-salicylic acid) and salicylic acid (analgesics and their derivatives) on the antibiotic resistance of ammonia oxidizing bacterium (aob) (a non-pathogenic environmental microbe) cultured from the texas tech university-water recovery system that treats a space related wastewater for nasa. the effect of salicylic acid was investigated by obtaining the minimal inhibition concentration (mic) of antibiotics (amo ... | 2009 | 19448319 |
| rapid detection of salmonella in milk by combined immunomagnetic separation-polymerase chain reaction assay. | during the past few years, milk has presented a risk of salmonella contamination; it has been implicated as the cause in several outbreaks of salmonellosis. because conventional detection methods require 5 to 7 d for completion and involve several subcultivation stages followed by biochemical and serological tests, rapid and sensitive methods have been sought, mainly at the dna level. therefore, a study including milk samples was conducted to evaluate the performance of a combination of 2 techni ... | 2009 | 19447970 |
| redundant hydrogen peroxide scavengers contribute to salmonella virulence and oxidative stress resistance. | salmonella enterica serovar typhimurium is an intracellular pathogen that can survive and replicate within macrophages. one of the host defense mechanisms that salmonella encounters during infection is the production of reactive oxygen species by the phagocyte nadph oxidase. among them, hydrogen peroxide (h(2)o(2)) can diffuse across bacterial membranes and damage biomolecules. genome analysis allowed us to identify five genes encoding h(2)o(2) degrading enzymes: three catalases (kate, katg, and ... | 2009 | 19447905 |
| salmonella typhimurium: insight into the multi-faceted role of the lysr-type transcriptional regulators in salmonella. | the lysr-type transcriptional regulators (lttrs) are widely distributed in various genera of prokaryotes. lttrs are dna binding proteins that can positively or negatively regulate target gene expression and can also repress their own transcription. salmonella enterica comprises a group of gram-negative bacteria capable of causing clinical syndromes that range from self-limiting diarrhoea to severe fibrinopurulent necrotizing enteritis and life threatening systemic disease. the survival and repli ... | 2009 | 19447191 |
| non-typhoidal salmonella bacteraemia: epidemiology, clinical characteristics and its' association with severe immunosuppression. | non-typhoidal salmonella (nts) is increasingly recognized as an important pathogen associated with bacteraemia especially in immunosuppressed patients. however, there is limited data specifically describing the clinical characteristics and outcome amongst the immunosuppressed patients. | 2009 | 19445730 |
| detection of salmonella enterica serovar typhimurium with puo-stvr2-like virulence-resistance hybrid plasmids in the united kingdom. | the aim of this study was to investigate the presence in the united kingdom (uk) of salmonella enterica serovar typhimurium isolates carrying puo-stvr2-like virulence-resistance hybrid plasmids that originated from pslt. one hundred and fifty ampicillin-resistant isolates of s. typhimurium, collected in different regions of the uk during 2006, were screened for the presence of bla (oxa-1) carried by an inh-like integron (2000 bp/bla (oxa-1)-aada1) characteristic of puo-stvr2. positive isolates w ... | 2009 | 19444492 |
| [characterization of attenuated salmonella c500 strain with a delta asd mutant and use as an asd+ balanced-lethal host-vector system]. | salmonella enterica serovar choleraesuis strain c500 is a live, attenuated vaccine that has been used in china for over 40 years to prevent piglet paratyphoid. the objective of this study was to evaluate the potential of attenuated salmonella enterica serovar choleraesuis c500 strain with a delta asd mutant as an effective live vaccine vector by the asd+ balanced-lethal host-vector system. here, we compared the characteristics of s. enterica serovar choleraesuis delta asdc500 strain with the par ... | 2009 | 19441223 |
| scamp3 is a component of the salmonella-induced tubular network and reveals an interaction between bacterial effectors and post-golgi trafficking. | salmonella enterica are facultative intracellular bacterial pathogens that proliferate within host cells in a membrane-bounded compartment, the salmonella-containing vacuole (scv). intracellular replication of salmonella is mediated by bacterial effectors translocated on to the cytoplasmic face of the scv membrane by a type iii secretion system. some of these effectors manipulate the host endocytic pathway, resulting in the formation in epithelial cells of tubules enriched in late endosomal mark ... | 2009 | 19438519 |
| characterization of mgtc, a virulence factor of salmonella enterica serovar typhi. | the mgtc is a virulence factor in salmonella typhimurium that is required for growth at low-mg2+ concentrations and intramacrophage survival. this gene is codified in a conserved region of the salmonella pathogenicity island 3 (spi-3), and is also present in the chromosome of other salmonella serovars. in this study we characterized the mgtc factor in s. typhi, a human specific pathogen, by using mgtc and spi-3 mutant strains. we found that mgtc is the most important factor codified in the spi-3 ... | 2009 | 19436747 |
| internalization of salmonella enterica serovar montevideo into greenhouse tomato plants through contaminated irrigation water or seed stock. | tomatoes have been linked to outbreaks of salmonellosis, demonstrating the need to identify sources of contamination. objectives of this study included determining the ability for salmonella enterica serovar montevideo to be internalized into tomatoes from contaminated irrigation water and seed stock, and establishing whether salmonella montevideo can survive in fertilizer solutions. six treatment groups (five plants per group) were irrigated with 350 ml of 7 log cfu/ml of salmonella montevideo ... | 2009 | 19435236 |
| perturbation of the small intestine microbial ecology by streptomycin alters pathology in a salmonella enterica serovar typhimurium murine model of infection. | the small intestine is an important site of infection for many enteric bacterial pathogens, and murine models, including the streptomycin-treated mouse model of infection, are frequently used to study these infections. the environment of the mouse small intestine and the microbiota with which enteric pathogens are likely to interact, however, have not been well described. therefore, we compared the microbiota and the concentrations of short-chain fatty acids (scfas) present in the ileum and cecu ... | 2009 | 19433544 |
| drug-resistant salmonella typhimurium dt 120: use of pfge and mlva in a putative international outbreak investigation. | isolates of salmonella enterica serovar typhimurium belonging to definitive phage type (dt) 120 (salmonella typhimurium dt 120) from simultaneous outbreaks of infection in the england and denmark have been compared on the basis of antibiogram, pulsed-field gel electrophoresis (pfge), and multiple locus variable number tandem repeat analysis (mlva). isolates from england had the resistance profile (ampicillin, streptomycin, sulfamethoxazole, and tetracycline), mlva profiles 2-4-4-0-2, 2-4-5-0-2, ... | 2009 | 19432517 |
| salmonella enterica outbreak in a banqueting hall in jerusalem: the unseen hand of the epidemiological triangle? | foodborne salmonella enterica outbreaks constitute both a threat to public health and an economic burden worldwide. | 2009 | 19432037 |
| isolation of salmonella typhimurium from dead blue and gold macaws (ara ararauna). | two blue and gold macaw (ara ararauna) chicks died of fatal salmonellosis in buenos aires province, argentina. the birds were histopathologically and microbiologically examined. salmonella enterica subspecies enterica serovar typhimurium was isolated from the liver, spleen, heart, lung, kidney, and intestine of both birds. all strains were susceptible to ampicillin, cephalothin, cefotaxime, enrofloxacin, nalidixic acid, gentamicin, streptomycin, chloramphenicol, fosfomycin, tetracycline, nitrofu ... | 2009 | 19432017 |
| potential role for the 4,12 antigen in the prevalence of clonal salmonella serovars in poultry. | 2009 | 19429568 | |
| sopb of salmonella enterica serovar typhimurium is a potential dna vaccine candidate in conjugation with live attenuated bacteria. | the immune response against salmonella is multi-faceted involving both the innate and the adaptive immune system. the characterization of specific salmonella antigens inducing immune response could critically contribute to the development of epitope based vaccines for salmonella. we have tried to identify a protective t cell epitope(s) of salmonella, as cell mediated immunity conferred by cd8+ t cells is the most crucial subset conferring protective immunity against salmonella. it being a proven ... | 2009 | 19428891 |
| effect of expression level on immune responses to recombinant oral salmonella enterica serovar typhimurium vaccines. | live, attenuated salmonella has been used to express heterologous antigens for development of oral vaccines. often, expression must be regulated because of deleterious effects on the salmonella vector. the effect of varying expression levels on immune response parameters has not been well defined. in this study we introduced mutations in the -10 region of the trc promoter in the expression plasmid to generate series of vaccine strains with different levels of expression of a model antigen, the h ... | 2009 | 19428883 |
| identifying promoter features of co-regulated genes with similar network motifs. | a large amount of computational and experimental work has been devoted to uncovering network motifs in gene regulatory networks. the leading hypothesis is that evolutionary processes independently selected recurrent architectural relationships among regulators and target genes (motifs) to produce characteristic expression patterns of its members. however, even with the same architecture, the genes may still be differentially expressed. therefore, to define fully the expression of a group of gene ... | 2009 | 19426448 |
| sanitation of faeces from source-separating dry toilets using urea. | to develop a reliable and simple method to produce safe fertilizers from human excreta using urea for sanitation of faeces. | 2009 | 19426280 |
| conjugative transfer of plasmid-located antibiotic resistance genes within the gastrointestinal tract of lesser mealworm larvae, alphitobius diaperinus (coleoptera: tenebrionidae). | the frequency of conjugative transfer of antimicrobial resistance plasmids between bacteria within the gastrointestinal tract of lesser mealworm larvae, a prevalent pest in poultry production facilities, was determined. lesser mealworm larvae were exposed to a negative bacterial control, a donor salmonella enterica serotype newport strain, a recipient escherichia coli, or both donor and recipient to examine horizontal gene transfer of plasmids. horizontal gene transfer was validated post externa ... | 2009 | 19425825 |
| serotype distribution and antibiotic resistance of human gastrointestinal isolates of salmonella enterica from crete, greece. | 2009 | 19423479 | |
| analysis of antimicrobial resistance and plasmid profiles in salmonella serovars associated with tropical seafood of india. | a total of 256 salmonella strains consisting of 29 salmonella serovars isolated from seafood of cochin (india) were analyzed for resistance to antimicrobials commonly used in human and veterinary medicines as therapeutic agents. the 10 most predominant salmonella serovars in seafood were also characterized for presence of plasmids using the alkaline lysis method. antimicrobial susceptibility studies highlighted a comparatively high resistance in salmonella isolates to sulfamethizol and carbenici ... | 2009 | 19422307 |
| effects of static magnetic field on cell growth, viability, and differential gene expression in salmonella. | in the present study, we investigated the effect of exposure to a static magnetic field (smf) on cell growth, viability, and gene expression of salmonella enterica subsp. enterica serovar hadar. our results indicated that smf exposure (200 mt, 13 hours) failed to alter cellular growth but induced a decrease of colony-forming units (cfu) between 3 and 6 hours followed by an increase from 6 to 9 hours. the analysis of the differential expression of rpoa, dnak, katn, and 16s rrna genes under smf ex ... | 2009 | 19422305 |
| the structure of a cytolytic alpha-helical toxin pore reveals its assembly mechanism. | pore-forming toxins (pfts) are a class of potent virulence factors that convert from a soluble form to a membrane-integrated pore. they exhibit their toxic effect either by destruction of the membrane permeability barrier or by delivery of toxic components through the pores. among the group of bacterial pfts are some of the most dangerous toxins, such as diphtheria and anthrax toxin. examples of eukaryotic pfts are perforin and the membrane-attack complex, proteins of the immune system. pfts can ... | 2009 | 19421192 |
| salmonella enterica serovar typhi live vector vaccines finally come of age. | attenuated salmonella typhi vaccine strains hold great promise as live vectors for presentation of foreign antigens from unrelated bacterial, viral and parasitic pathogens to the immune system. although this approach has proved quite successful in experimental animal models for eliciting antigen-specific mucosal, humoral and cellular responses, results have been disappointing for clinical trials carried out thus far. we hypothesize that the paucity of human responses to foreign antigens delivere ... | 2009 | 19417771 |
| comparison of genetic and physiological properties of salmonella enterica isolates from chickens reveals one major difference between serovar kentucky and other serovars: response to acid. | for unknown reasons, salmonella enterica kentucky has become the serovar most frequently isolated from chickens and chicken carcasses in the united states. in an attempt to identify traits that may underlie this phenomenon, genetic and physiological features of 30 serovar kentucky chicken isolates were compared with those of chicken isolates belonging to a range of other s. enterica serovars. most of the well-known salmonella virulence genes were detected in the serovar kentucky isolates by pcr, ... | 2009 | 19415975 |
| a mutant of salmonella enterica serovar typhimurium rna polymerase extracytoplasmic stress response sigma factor sigma(e) with altered promoter specificity. | the alternative sigma factor sigma(e) is critical for envelope stress response and plays a role in pathogenicity of a variety of different bacteria. we previously identified several critical nucleotides in the salmonella enterica serovar typhimurium (s. typhimurium) sigma(e)-dependent rpoep3 promoter that corresponded to the most conserved nucleotides in the sigma(e) consensus sequence of the -10 and -35 promoter elements. in the present study, we exploited a previously established escherichia c ... | 2009 | 19415331 |
| a genetic analysis of in vivo selenate reduction by salmonella enterica serovar typhimurium lt2 and escherichia coli k12. | the twin-arginine transport (tat) system is dedicated to the translocation of folded proteins across the bacterial cytoplasmic membrane. proteins are targeted to the tat system by signal peptides containing a twin-arginine motif. in salmonella enterica serovar typhimurium and escherichia coli many tat substrates are known or predicted to bind a molybdenum cofactor in the cytoplasm prior to export. in the case of n- and s-oxide reductases, co-ordination of molybdenum cofactor insertion with prote ... | 2009 | 19415239 |
| evaluation of iso enrichment real-time pcr methods with internal amplification control for detection of listeria monocytogenes and salmonella enterica in fresh fruit and vegetables. | to provide with a quick method for qualitative detection, in less than three days, of salmonella enterica and listeria monocytogenes in fresh fruit and vegetables. | 2009 | 19413765 |
| adaptation and cross-adaptation of listeria monocytogenes and salmonella enterica to poultry decontaminants. | information on the potential for acquired reduced susceptibility of bacteria to poultry decontaminants occurring is lacking. minimal inhibitory concentrations (mics) were established for assessing the initial susceptibility and the adaptative and cross-adaptative responses of four bacterial strains (listeria monocytogenes serovar l/2a, l. monocytogenes serovar 4b, salmonella enterica serotype typhimurium, and s. enterica serotype enteritidis) to four poultry decontaminants (trisodium phosphate, ... | 2009 | 19412596 |
| the global consequence of disruption of the acrab-tolc efflux pump in salmonella enterica includes reduced expression of spi-1 and other attributes required to infect the host. | the mechanisms by which rnd pumps contribute to pathogenicity are currently not understood. using the acrab-tolc system as a paradigm multidrug-resistant efflux pump and salmonella enterica serovar typhimurium as a model pathogen, we have demonstrated that acra, acrb, and tolc are each required for efficient adhesion to and invasion of epithelial cells and macrophages by salmonella in vitro. in addition, acrb and tolc are necessary for salmonella to colonize poultry. mutants lacking acra, acrb, ... | 2009 | 19411325 |
| the dna static curvature has a role in the regulation of the omps1 porin gene in salmonella enterica serovar typhi. | the dna static curvature has been described to play a key role as a regulatory element in the transcription process of several bacterial genes. here, the role of dna curvature in the expression of the omps1 porin gene in salmonella enterica serovar typhi is described. the web server mutacurve was used to predict mutations that diminished or restored the extent of dna curvature in the 5' regulatory region of omps1. using these predictions, curvature was diminished by site-directed mutagenesis of ... | 2009 | 19406898 |
| rfab, a galactosyltransferase, contributes to the resistance to detergent and the virulence of salmonella enterica serovar enteritidis. | in this study, a deletion mutant of rfab (deltarfab) was observed to be susceptible to sodium dodecyl sulfate and less tolerant to bile salts. in addition, pre-incubation in 10% bile salts increased bacterial tolerance to 30% bile salts. we also showed that the deltarfab mutant invaded hela cells less than the wild type and resulted in a lower ratio of intracellular bacteria. competitive infection of mice showed that the deltarfab mutant was defective in the colonization of host organs and was c ... | 2009 | 19404677 |
| characterization of the enzyme aac(3)-id in a clinical isolate of salmonella enterica serovar haifa causing traveler's diarrhea. | the objective of this investigation was to identify the mechanism of decreased susceptibility to gentamicin in a salmonella clinical isolate, leading to the detection of a aminoglycoside acetyltransferase gene found in a class 1 integron. | 2009 | 19403206 |
| reduced fluoroquinolone susceptibility in salmonella enterica isolates from travelers, finland. | we tested the fluoroquinolone susceptibility of 499 salmonella enterica isolates collected from travelers returning to finland during 2003-2007. among isolates from travelers to thailand and malaysia, reduced fluoroquinolone susceptibility decreased from 65% to 22% (p = 0.002). all isolates showing nonclassical quinolone resistance were from travelers to these 2 countries. | 2009 | 19402977 |
| an oral recombinant salmonella enterica serovar typhimurium mutant elicits systemic antigen-specific cd8+ t cell cytokine responses in mice. | the induction of antigen-specific cd8+ t cell cytokine responses against an attenuated, oral recombinant salmonella enterica serovar typhimurium vaccine expressing a green fluorescent protein (gfp) model antigen was investigated. a gfp expression plasmid was constructed in which the gfp gene was fused in-frame with the 5' domain of the escherichia coli beta-galactosidase alpha-gene fragment with expression under the lac promoter. groups of mice were orally immunized three times with the bacteria ... | 2009 | 19402893 |
| two site-directed mutations are required for the conversion of a sugar dehydratase into an aminotransferase. | l-colitose and d-perosamine are unusual sugars found in the o-antigens of some gram-negative bacteria such as escherichia coli, vibrio cholerae, and salmonella enterica, among others. the biosynthetic pathways for these two sugars begin with the formation of gdp-mannose from d-mannose 1-phosphate and gtp followed by the subsequent dehydration and oxidation of gdp-mannose to yield gdp-4-keto-6-deoxymannose. following the production of gdp-4-keto-6-deoxymannose, the two pathways diverge. in the ca ... | 2009 | 19402712 |
| [molecular subtyping of salmonella enterica serotype enteritidis in a post epidemic period]. | in the last two decades, salmonella enterica serotype enteritidis has become one of the main agents causing food borne diseases worldwide. this agent is transmitted mainly by contaminated meat and poultry. | 2009 | 19399324 |
| a typhoid fever outbreak in a slum of south dumdum municipality, west bengal, india, 2007: evidence for foodborne and waterborne transmission. | in april 2007, a slum of south dumdum municipality, west bengal reported an increase in fever cases. we investigated to identify the agent, the source and to propose recommendations. | 2009 | 19397806 |
| antimicrobial efficiency of essential oil and freeze-thaw treatments against escherichia coli o157:h7 and salmonella enterica ser. enteritidis in strawberry juice. | this study investigated the antimicrobial efficiency of 3 essential oils (eos), lemongrass, cinnamon leaf, and basil, and freeze-thaw treatment, alone or in combination, against escherichia coli o157:h7 and salmonella enterica ser. enteritidis inoculated in strawberry juice stored at 7 degrees c. eo of lemongrass or cinnamon leaf at 0.1 to 2 microl/ml and freezing at -23 degrees c for 24 or 48 h followed by thawing at 7 degrees c for 4 h all showed significant antimicrobial activities (p < 0.05) ... | 2009 | 19397729 |
| salmonella enterica subsp. arizonae bone and joints sepsis. a case report and literature review. | osteoarticular infections caused by salmonella enterica subsp. arizonae are rarely seen in humans but young children and immunocompromised adults are at particular risk of acquiring this bacteria. reptiles and their by-products (e.g. meat preparations or medications) are particularly likely to harbor salmonella. we report on a case of septic arthritis of the hip transmitted by a reptile in a 10-month-old child. we carry out a recall of the complex nomenclature of salmonella, a review of the lite ... | 2009 | 19395336 |
| population structure, origins and evolution of major salmonella enterica clones. | the genus salmonella consists of two species s. enterica and s. bongori. s. enterica has a well defined subspecies structure with seven subspecies consistently delineated by sequence variation. frequency of recombination between subspecies and within a subspecies is markedly different. subspecies i undergoes frequent recombination as demonstrated recently, demystifying the long-held belief that salmonella is a highly clonal organism. the majority of disease causing serovars are from subspecies i ... | 2009 | 19393770 |
| genotoxic activation of the environmental pollutant 3,6-dinitrobenzo[e]pyrene in salmonella typhimurium umu strains expressing human cytochrome p450 and n-acetyltransferase. | 3,6-dinitrobenzo[e]pyrene (dnbep) is a potent mutagen identified in surface soil in two metropolitan areas of japan. we investigated whether dnbep can cause genotoxicity through any metabolic activation pathway in bacteria using the parental strain salmonella enterica serovar typhimurium (s. typhimurium) ta1535/psk1002, nitroreductase (nr)-deficient strain nm1000, the o-acetyltransferase (o-at)-deficient strain nm2000, bacterial o-at-overexpressing strain nm2009, and bacterial nr- and o-at-overe ... | 2009 | 19393727 |
| microbial co-habitation and lateral gene transfer: what transposases can tell us. | determining the habitat range for various microbes is not a simple, straightforward matter, as habitats interlace, microbes move between habitats, and microbial communities change over time. in this study, we explore an approach using the history of lateral gene transfer recorded in microbial genomes to begin to answer two key questions: where have you been and who have you been with? | 2009 | 19393086 |
| search for salmonella spp. in ostrich productive chain of brazilian southeast region. | we analyzed ostriches from an equipped farm located in the brazilian southeast region for the presence of salmonella spp. this bacterium was investigated in 80 samples of ostrich droppings, 90 eggs, 30 samples of feed and 30 samples of droppings from rodents. additionally, at slaughter-house this bacterium was investigated in droppings, caecal content, spleen, liver and carcasses from 90 slaughtered ostriches from the studied farm. also, blood serum of those animals were harvested and submitted ... | 2009 | 19390983 |
| accurate prediction of secreted substrates and identification of a conserved putative secretion signal for type iii secretion systems. | the type iii secretion system is an essential component for virulence in many gram-negative bacteria. though components of the secretion system apparatus are conserved, its substrates--effector proteins--are not. we have used a novel computational approach to confidently identify new secreted effectors by integrating protein sequence-based features, including evolutionary measures such as the pattern of homologs in a range of other organisms, g+c content, amino acid composition, and the n-termin ... | 2009 | 19390620 |
| proteomic analysis of the adaptive response of salmonella enterica serovar typhimurium to growth under anaerobic conditions. | in order to survive in the host and initiate infection, salmonella enterica needs to undergo a transition between aerobic and anaerobic growth by modulating its central metabolic pathways. in this study, a comparative analysis of the proteome of s. enterica serovar typhimurium grown in the presence or absence of oxygen was performed. the most prominent changes in expression were measured in a semiquantitative manner using difference in-gel electrophoresis (dige) to reveal the main protein factor ... | 2009 | 19389776 |
| ferrous iron-binding protein omb of salmonella enterica serovar choleraesuis promotes resistance to hydrophobic antibiotics and contributes to its virulence. | salmonella enterica serovar choleraesuis (sc) is an important enteric pathogen that causes serious systemic infections in swine and humans. to identify the genes required for resistance to antimicrobial peptides, we constructed a bank of sc transposon mutants and screened them for hypersensitivity to the cationic peptide polymyxin b. here we report one isolated polymyxin b-susceptible mutant that also exhibited increased sensitivity toward human neutrophil peptide alpha-defensin 1 (hnp-1) and hy ... | 2009 | 19389759 |
| adhesion and growth inhibitory effect of chicken egg yolk antibody (igy) on salmonella enterica serovars enteritidis and typhimurium in vitro. | the protective effects of powder preparation of egg yolk immunoglobulin y (igy), specific to salmonella enteritidis and salmonella typhimurium outer membrane proteins (omp), against these two salmonella sp. serovars were investigated in vitro in two different assays: adhesion-prevention and growth-inhibition. the adhesion-prevention assay was conducted using polarized monolayers of the human intestinal epithelial caco-2 cell line. first, the conditions of salmonella adherence to caco-2 cells wer ... | 2009 | 19388827 |
| multilocus variable-number tandem-repeat method for typing salmonella enterica serovar newport. | in recent years, the proportion of salmonella enterica infections represented by s. enterica serovar newport has increased markedly among humans and animals. multilocus variable-number tandem-repeat analysis (mlva) has proven to be useful in discriminating other highly clonal salmonella serovars. here, we report on the development of a highly discriminatory mlva for salmonella serovar newport. | 2009 | 19386855 |
| fimh alleles direct preferential binding of salmonella to distinct mammalian cells or to avian cells. | this study aimed to determine whether allelic variants of the fimh adhesin from salmonella enterica confer differential bacterial binding to different types of mammalian cells [murine bone marrow-derived dendritic cells (dcs) and hep-2 cells] and chicken leukocytes. although the type 1 fimbriated s. enterica serovar typhimurium strains ajb3 (sr-11 derivative) and sl1344 both aggregated yeast cells, only the former bound efficiently to dcs and hep-2 cells. type 1 fimbriae-mediated binding to dcs ... | 2009 | 19383701 |
| b cell precursors are targets for salmonella infection. | we previously reported that, in mice, b cells are a reservoir for bacteria during salmonella infection. here, we show that, within the bone marrow, b cells and their precursors are targeted for infection by salmonella enterica serovar typhimurium. our data suggest that b cells within the bone marrow may be a bacterial niche during chronic salmonella infection. | 2009 | 19383536 |
| predictive value of clinical and laboratory findings in the diagnosis of the enteric fever. | although the definitive diagnosis of enteric fever requires the isolation of salmonella enterica serotype typhi or paratyphi, the diagnosis is usually made according to clinical and laboratory findings. there is usually a diagnostic dilemma. the aim of this study was to determine the minimum required parameters that could be valuable in the diagnosis of enteric fever. a retrospective study was performed to compare the clinical and laboratory findings in 60 patients who proved to have enteric fev ... | 2009 | 19382666 |