Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| identification of a mycobacterium leprae specific protein antigen(s) and its possible application for the serodiagnosis of leprosy. | acetone-killed mycobacterium leprae separated from infected armadillo liver tissue without the use of proteases were treated with 0.2 m lithium acetate, 20 mm edta, ph 8.8 solution, and the concentrated antigen extract was analyzed by ouchterlony immunodiffusion. the antigen extract gave a single immunoprecipitate when reacted with pooled lepromatous leprosy (ll) patients sera made highly specific for m. leprae by adsorption. apparently identical precipitates were produced by reacting the antige ... | 1979 | 90666 |
| leprosy's footprints in bone marrow. | 1979 | 88011 | |
| leprosy's footprints in bone-marrow histiocytes. | 1979 | 85259 | |
| cross-resistance amongst thiambutosine, thiacetazone, ethionamide and prothionamide with mycobacterium leprae. | 1978 | 84317 | |
| inhibition of rubino factor as a test for detecting antigens common to leprosy bacilli. | 1978 | 83310 | |
| comparison of various preparations of mycobacterium leprae and other mycobacteria by lymphocyte stimulation. | peripheral blood lymphocytes were stimulated in vitro with different mycobacterial antigen preparations and responses were measured as incorporation of tritiated thymidine. blood donors were 9 patients with lepromatous leprosy, 16 tuberculoid leprosy patients, and 6 healthy individuals with different degrees of exposure to leprosy. the results revealed a good correlation between the responses to m. leprae from human sources and bacilli from armadillos inoculated with human leprosy bacilli, altho ... | 1978 | 83309 |
| further studies of the action of antithyroid drugs on mycobacterium leprae. | 1978 | 80802 | |
| in-vitro lymphoproliferative response to mycobacterium leprae of hla-d-identical siblings of lepromatous leprosy patients. | lymphoproliferative responses to mycobacterium leprae and p.p.d. were measured in 23 lepromatous and borderline lepromatous leprosy patients and in 27 of their normal siblings. at the same time siblings hla-d-identical with the patients were identified by the absence of a mixed-lymphocyte reaction. the 7 siblings who were hla-identical to lepromatous patients responded as well to m. leprae as did the 20 hla-non-identical normal siblings. in contrast, 22 of the 23 lepromatous patients failed to r ... | 1978 | 79915 |
| two methods of demonstrating leprosy bacilli in smears. | two methods, the carbol fuchsin with acetic acid differentiation and the periodic acid-carbol pararosaniline, were used for demonstrating leprosy bacilli in skin smears. bacillary smears from 200 long-treated patients with tuberculoid, borderline and lepromatous leprosy were stained with periodic acid-carbol pararosaniline. there were significantly greater bi and mi determinations than with classic carbol fucsin staining. with the former stain bacilli were found in 69 of 96 skin smears in which ... | 1978 | 79561 |
| therapeutic effect of yeast glucan in mice infected with mycobacterium leprae [proceedings]. | 1977 | 79384 | |
| the activity of thiacetazone, thiambutosine, thiocarlide and sulphamethoxypyridazine against mycobacterium leprae in mice. | 1978 | 78428 | |
| mycobacterium leprae specific antibodies detected by radioimmunoassay. | a radioimmunoassay was developed for demonstration of antibodies against m. leprae specific antigenic determinants. the specificity of the assay was tested with hyperimmune rabbit antisera against other mycobacteria and shown to be very high. the titre of m. leprae specific antibodies in a lepromatous serum pool was 10(5). sixty-one of sixty-two lepromatous sera, all of twelve borderline sera and twenty of forty-eight tuberculoid sera were positive in the assay, whereas all of thirty-eight cont ... | 1978 | 77549 |
| rapid test for drug resistance in leprosy. | 1977 | 72940 | |
| a possible dysfunction of melanosome transfer in leprosy: an electron-microscopic study. | an e.m. study was carried out to investigate whether mycobacterium leprae occur intracellularly in epidermal melanocytes. as this could not be confirmed, the selective killing of melanocytes by cytotoxic lymphocytes could not explain the hypopigmentation in types of leprosy with a relative good immune response. there were indications that these hypopigmented lesions resulted from a disturbed transfer of melanosomes from melanocytes to keratinocytes. further research is in progress. | 1977 | 70920 |
| recent advances in microbiology in leprosy. | the recent advances in microbiology of leprosy are reviewed. till now the leprosy bacillus had not been cultivated in laboratory media; the recent claims of success have not been confirmed. there has been a breakthrough in the experimental transmission of leprosy to experimental animals--the white mice, the immune depressed white mice, and the nine-banded armadillo. apart from providing definite proof for the causative relationship of the bacillus discovered by hansen and the disease leprosy, th ... | 1977 | 70571 |
| common antigen of mycobacterium leprae, m. lepraemurium, m. avium, and m. fortuitum in comparative studies using two different types of antisera. | no. 21 mycobacterial antigens of mycobacterium lepraemurium, m. avium, m. fortuitum, and m. leprae were compared in crossed immunoelectrophoresis using two different antibody sources, a serum pool from lepromatous leprosy patients (lsii) and a rabbit anti-m. smegmatis antiserum. m. lepraemurium, like m. avium, was found to contain the 21 a and 21 c determinants. m. fortuitum contained in addition a new type of determinant, 21 d.m. leprae antigen no. 21 carried the a as well as the b dertminants, ... | 1977 | 68004 |
| m. leprae versus m. scrofulaceum. | 1976 | 66215 | |
| cultivation of mycobacterium leprae. | 1977 | 64727 | |
| germicidal activity of the pigo system on mycobacterium leprae, in vitro. | 1976 | 64181 | |
| hla-linked genetic control of host response to mycobacterium leprae. | non-random parental hla-haplotype segregation is demonstrated in siblings with leprosy. a new method is described for the statistical analysis of non-random segregation among sibships of different sizes. sibs with the same type of leprosy show a significant excess of identical hla haplotypes. this is also true for families in which only tuberculoid leprosy is found, which is by far the commonest type in the population studied. however, sibs affected with different types of leprosy share a haplot ... | 1976 | 63804 |
| rifampin-resistant leprosy. | 1976 | 63780 | |
| histocompatibility antigens in patients with leprosy. | the frequencies of distribution of 25 histocompatibility antigens were determined in 92 mexican patients with leprosy and compared with those in 315 mexicans who did not have the disease. no statistically significant differences were found between the patients and the controls in regard to histocompatibility antigens, and subgroups with a significant difference could not be identified by division of the patients according to the density of mycobacterium leprae or the presence or absence of cell- ... | 1976 | 63527 |
| periodic acid-methenamine silver stain for mycobacteria in tissue sections. | 1976 | 63164 | |
| the nature of mycobacterial acid-fastness. | phenol is not essential to acid-fast staining, for it will occur in the absence of phenol where such lipoid-soluble basic dyes as night blue, victoria blue b or victoria r are used; it is essential for acid-fast staining with water soluble basic dyes such as basic fuchsin. when phenol is added to the staining solution, such water soluble basic dyes behave in effect like their lipid-soluble counterparts. the loss of mycobacterial acid-fastness with carbol-fuchsin after bromination or chromation i ... | 1976 | 63160 |
| the use of non-deparaffinized tissue sections for staining leprosy bacilli. | reduced acid-fast staining of leprosy bacilli occurs during the dewaxing of paraffin sections by xylene and alcohols; the older and more decrepit bacilli being especially affected. by the use of non-deparaffinized sections, the leprosy bacilli which could not be stained with the usual carbol fuchsin are strongly stained. moreover, non-deparafinized sections can be used for the periodic acid-carbol pararosanilin stain or methenamine silver stain for demonstrating mycobacteria. | 1976 | 61950 |
| immunologic identification of m. leprae. immunofluorescence and complement fixation. | a markedly improved immunofluorescent technic employing fitc conjugated igg antibody prepared from lepromatous serum is described as a means of specific identification of m. leprae. an additional immunologic identification method for m. leprae is presented as a micro-complement fixation technic employing antigen rather than antibody dilution. studies with these technics suggest that m. leprae specific antigen is probably a surface antigen and has as part of its mosaic a lecithin-phospholipid com ... | 1976 | 61949 |
| enumeration of mycobacterium leprae stained with and without prior periodate oxidation. | 1976 | 61553 | |
| letter: culture of m. leprae with help of desiccated thyroid. | 1976 | 60588 | |
| acid-fast properties and pyridine extraction of m. leprae. | the reportedly unique pyridine extractability of acid-fastness as an identifying characteristic for m. leprae was examined in the leprosy bacilli and in eight other strains of mycobacteria. the initial findings were, in general, in accord with previous reports except that m. smegmatis and m. phlei likewise demonstrated two hour pyridine extractability of acid-fastness. perhaps, more significantly, it was found that this characteristic in m. leprae is related to aged, probably nonviable bacilli. ... | 1975 | 58845 |
| mycobacterial antigens in antibody responses of leprosy patients. | a reference system for m. smegmatis antigens in crossed immunoelectrophoresis was used to study antibody activities in serum samples of 91 leprosy patients. all polar and borderline lepromatous patients were positive. mean numbers out of 14 m. smegmatis antigens involved were 4.3 and 3.5, respectively. precipitins against antigen no. 1 were seen in all lepromatous cases. antibodies against this antigen were detected in 50% of tuberculoid (polar, subpolar and borderline) cases. antibody activity ... | 1975 | 58844 |
| studies of mycobacterial antigens, with special reference to mycobacterium leprae. | eight individual antigens were detected in soluble antigen preparations from mycobacterium leprae bacilli by using pools of serum samples from lepromatous leprosy patients as antibody reagents in crossed immunoelectrophoresis. two of these antigens were analyzed further. antgent no. 1 gave an elution pattern on sephadex g-200 corresponding to a molecular weight of 285,000. this antigen was also present in three slow-growing and eight fast-growing mycobacterial species. there was a reaction of co ... | 1976 | 58837 |
| staining mycobacteria with carbolfuchsin: properties of solutions prepared with different samples of basic fuchsin. | acid fast staining of mycobacteria in the form of beadings is obtained by means of a carbolfuchsin solution (ziehl-neelsen stain) prepared from pararosaniline or from certain kinds of basic fuchsin. after such acid-fast stains, the intensity of the bacilli's colouring was rather poor and unstable, so that some bacilli lost their acid-fast stain. in contrast, an acid-fast staining of mycobacteria in rod form results by using a carbolfuchsin prepared from rosaniline or from other basic fuchsins in ... | 1976 | 58364 |
| editorial: the nose and leprosy. | 1976 | 57462 | |
| antigenic heterogeneity in patients with reactions in borderline leprosy. | fifteen patients with borderline leprosy who developed "reversal" reactions were studied from the inception of treatment. thirteen showed an appreciable increase in lymphocyte transformation (lt) when preparations of mycobacterium leprae were used as antigen. the lt responses to either "whole" or "sonicated" preparations of the bacillus in these 15 patients and in nine others also in reaction correlated with the clinical presentation. those with skin disease predominating in the reaction showed ... | 1975 | 53086 |
| long-spacing collagen in skin biopsies from patients with lepromatous leprosy. | in the course fo evaluating the effect of different drugs on the morphology of mycobacterium leprae (edwards, draper & draper, 1972) and on the phagocytic cells containing these organisms (edwards, 1973), an unusual feature was observed in the dermal connective tissue of skin biopsies obtained from some of the patients. variable amounts of an extracellular, cross-striated osmiophilic material were present in the dermis. the purpose of this communication is to describe the banded substance observ ... | 1975 | 51648 |
| innervation of muscle in leprosy with special reference to the muscle spindle. | the pattern of extrafusal and intrafusal innervation was studied in muscle biopsies from the flexor carpi ulnaris and biceps brachii muscles, which were clinically unimpaired. smudginess and enlargement of the motor end plates were the most definitely abnormal feature of the extrafusal innervation pattern; the intrafusal fibers, on the other hand, were either unremarkable or showed increased tortuosity, beading and in more extreme cases of grossly complicated intertwining pattern. bacilli were f ... | 1975 | 51013 |
| letter: sulphone resistance in leprosy. | 1975 | 49763 | |
| sulphone resistance in leprosy. a review of one hundred proven clinical cases. | an account is given of the first hundred consecutive proven cases of sulphone resistance in leprosy, detected in malaysia between 1963 and 1974. proof of resistance was clinical in eighty patients and was obtained by drug-sensitivity testing in mice in ninety-six patients; 76 cases were proved both clinically and experimentally, and there was no discrepancy between the two methods. sulphone resistance was confined to patients with lepromatous-type leprosy--i.e., patients with a large bacterial p ... | 1975 | 49662 |
| inhibition of multiplication of mycobacterium leprae by several antithyroid drugs. | multiplication of mycobacterium leprae in the mouse footpad was inhibited when mice were fed, mixed in their diet, 0.05 per cent methimazole, 0.066 per cent usp thyroid powder, methimazole plus thyroid powder, 0.15 per cent 5-n-heptyl-2-thioxo-4-thiazolidinone, 0.1 per cent propylthiouracil, and 0.1 per cent thambutosine for 154 days, beginning on the day of inoculation. all of the treatment regimens, except for the 2 containing thyroid powder, decreased the plasma concentrations of thyroxine an ... | 1975 | 48349 |
| in vitro cultivation of leprosy bacilli in hyaluronic acid-based medium. 2. progress and developing concept of the role of hyaluronic acid suggested by culture and armadillo infection studies. | progress is summarized relating to the verification, identification of m. leprae and understanding of the process of adaptation the pathogen passes through before in vitro growth takes place. it is recognized that hyaluronic acid apparently does not serve as a source of energy but the possibility is presented that it plays a role in the reconstruction of m. leprae cell walls made "leaky" by constant intracellular life. this apparently occurs, in culture, initially by the development of coccoid f ... | 1978 | 32143 |
| i.c.m.r. conference on leprosy research. | 1978 | 29989 | |
| symposium on recent progress on immunology of leprosy and chronic mycobacterial infections. | 1978 | 29988 | |
| immunity to mycobacterium leprae infections in mice stimulated by m. leprae, bcg, and graft-versus-host reactions. | infections of mice with mycobacterium leprae in one rear foot pad immunized them against a second infection in the other rear foot pad. purified bacilli harvested from the first infection also produced immuniy when injection into the foot pads of previously uninfected mice. injections of bcg afforded similar protection, but had no adjuvant effect on m. leprae. m. duvali, a cultivable mycobacterium that is reported to be more closely related antigenically to m. leprae than bcg is, provided much l ... | 1976 | 11189 |
| recent advances in experimental leprosy. | within the last 15 years we have learned to identify mycobacterium leprae, determine its viability, screen the efficacy of antileprosy drugs, and monitor the bacilli for drug sensitivity. we have evidence that subclinical infections occur frequently among contacts of patients with leprosy and that the different manifestations of leprosy reflect differences in resistance to m leprae. we are developing hypotheses about the mechanism of these differences. we have experimentally transmitted lepromat ... | 1976 | 8844 |