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[transformation of bacillus cereus protoplasts with the plasmid dna from bacillus thuringiensis transformants]. 19836417981
isolation of a dna sequence related to several plasmids from bacillus thuringiensis after a mating involving the streptococcus faecalis plasmid pam beta 1.the transmissible plasmid pam beta 1, which codes for resistance to erythromycin and lincomycin, was transferred from streptococcus faecalis to several strains of bacillus thuringiensis by a filter-mating process. introduction of pam beta 1 into the emr transconjugant strains of b. thuringiensis was confirmed by southern hybridisation using the 32p-labelled pam beta 1 as a probe. in the b. thuringiensis transconjugant strains, used as donors, the beta plasmid conserved its ability to be transfer ...19836413823
distribution of heterogeneous and homologous plasmids in bacillus spp.a total of 75 strains (including 5 reference strains) of bacillus amyloliquefaciens, b. cereus, b. circulans, b. licheniformis, b. megaterium, b. pumilus, b. sphaericus, b. subtilis, and b. thuringiensis and 36 species-unidentified bacillus strains were surveyed for plasmids by cesium chloride-ethidium bromide equilibrium centrifugation of cell lysates in a study of antibiotic resistance in host cells. of the 111 strains, 13 (including 3 reference strains) were found to harbor plasmids, and 5 of ...19836410988
[experiments with bacillus thuringiensis protoplasts. ii. study of the potential interspecies fusion of bacterial protoplasts: bacillus thuringiensis and bacillus megaterium].in the present study the possibility of obtaining interspecific bacterial hybrids by polyethylene glycol-assisted fusion of protoplasts from bacillus thuringiensis and bac. megaterium has been examined. electron microscopic and genetic data allow to confirm with great probability that cytological fusion takes place. however, genetic analysis revealed that neither of methods applied for protoplast fusion gave stable recombinants. apparently, it is due to the lack of recombination or the death of ...19836407894
alkaline extraction of toxin from spores of the mosquito pathogen, bacillus sphaericus strain 1593.toxin was extracted from spores of the mosquito pathogen bacillus sphaericus strain 1593 using 0.05 m naoh. the molecular weight of this toxin was 35000-54000. toxic activity of this extract was resistant to a variety of enzymes including subtilisin, but was degraded by pronase. antiserum produced to 1593 spore toxin neutralized spore toxin and cytoplasmic toxin activity, but did not react with bacillus thuringiensis var. israelensis crystal toxin, nor did var. israelensis toxin antiserum react ...19836406023
bioinsecticides: i. bacillus thuringiensis. 19846399844
[molecular genetic studies of the dna promotor regions in bacillus thuringiensis subsp. galleriae 69-6. i. structural and functional analysis].three recombinant plasmids ppbt9, ppbt10 and ppbt74 carrying promoter-containing regions of dna of bacillus thuringiensis which are responsible for the expression of the promoterless tet gene, were studied. in the in vitro experiments, it had been shown that these promoter-active hindiii fragments of bacillar dna contained rna polymerase binding sites. the alui subfragments that specifically bind to escherichia coli rna polymerase promote the tet gene expression, similar to the whole hindiii fra ...19846389259
[insecticidal endotoxin produced by bacillus thuringiensis--recent progress in the molecular biology and the future development]. 19846385143
[analysis of the changes in the electrophysical and morphometric parameters in the growth and sporulation periods of bacillus thuringiensis].changes in the electrophysical and morphometrical parameters of bacillus thuringiensis cells were studied during their growth and differentiation using the electrophysical procedure with the frequency of an alternating electric field varying within a wide range. the appearing variations were interpreted on the basis of the data about changes in the concentration of vegetative cells and spores, the optical density and ph of the cultural broth, and the assimilation of glucose by the culture. the c ...19846379383
[effect of proteins on exoprotease synthesis by bacillus thuringiensis].bacillus thuringiensis was shown to grow better in media with albumin, gelatin and casein than in a chemically defined medium; proteins did not induce the synthesis of exoprotease. two maxima were found in the enzyme synthesis: the first one at the exponential phase of the cultural growth, and the second one during spore formation by the culture. the synthesis at the exponential growth phase was susceptible to nitrogen metabolite repression while the synthesis of exoprotease at the stationary ph ...19836358805
two types of entomocidal toxins in the parasporal crystals of bacillus thuringiensis kurstaki.two types of entomocidal proteins of bacillus thuringiensis kurstaki were isolated from the parasporal bodies (crystals), and their structures were compared with each other in relation to the toxic activity. when the crystals were dissociated in 2% 2-mercaptoethanol at ph 10, a protein of mr = 135,000, called delta-endotoxin, was liberated. the crystals of a strain of b. thuringiensis kurstaki, the hd-1 strain, also released another protein in small quantities. this minor component of hd-1, whic ...19836357098
[activity of intracellular enzymes in bacillus thuringiensis prospores and sporangia].the activity of endocellular enzymes (alkaline phosphatase, protease, glucose dehydrogenase, aldolase, malate dehydrogenase, nadh dehydrogenase, nadh oxidase) was studied in isolated prospores and sporangia as well as in vegetative cells of bacillus thuringiensis strains, one of which produced crystals and one did not. the activity of malate dehydrogenase and nadh dehydrogenase was high in prospores of the both strains at the fifth and sixth stages of spore formation. the strain which did not pr ...19836341786
cloning and expression of the crystal protein genes from bacillus thuringiensis strain berliner 1715.from a clone bank of the entire genome of bacillus thuringiensis, one clone that contains a plasmid ( pbt 15-88) harboring a sporulation gene was identified by molecular hybridization. this gene, identified as the crystal protein gene, occurs both on a large host plasmid dna and in the chromosomal dna in b. thuringiensis strain berliner 1715. the inserted sequence of pbt 15-88, which corresponds to the chromosomal sequence, was not expressed in escherichia coli. in b. thuringiensis (kurstaki), t ...19826329704
[site-specific restriction endonuclease from a strain of bacillus thuringiensis]. 19846323119
specificity of cultured insect tissue cells for bioassay of entomocidal protein from bacillus thuringiensis.cultured tissue cells from lepidopteran and dipteran sources displayed an order-specific response to entomocidal protein from crystals of bacillus thuringiensis. protein isolated from crystals of b. thuringiensis subsp. kurstaki was effective against cells of the spruce budworm (choristoneura fumiferana) and the tobacco hornworm (manduca sexta), but was inactive against both mosquito cell lines tested (aedes aegypti and anopheles gambiae). conversely, protein from inclusion bodies of b. thuringi ...19846321329
potassium ion transport atpase in insect epithelia.k+ transport by the epithelia of midgut, salivary glands, malpighian tubules, sensory sensilla, possibly rectum, and other organs of certain insects appears to use a unique k+ atpase. ouabain inhibition of transport-related events has not been demonstrated in these epithelia. the k+ pump is unlike the na-k;ump but resembles the h;ump of phosphorylating membranes in its transport orientation, efficient thermodynamics, speculated two k+ per one mgatp2- stoichiometry, electrogenicity, and structure ...19836317792
cloning and localization of the lepidopteran protoxin gene of bacillus thuringiensis subsp. kurstaki.bacillus thuringiensis subsp. kurstaki produces a proteinaceous crystalline inclusion that is toxic for lepidopteran larvae. there are several size classes of plasmids in this organism and the presence of one or more has been correlated with production of this protein, defined as a protoxin. dna fragments of b. thuringiensis subsp. kurstaki, obtained by ecori digestion, were cloned into the vector charon 4a. recombinant phage were screened immunologically for the production of protoxin. cells in ...19826310560
in vitro transcription of the cloned chromosomal crystal gene from bacillus thuringiensis.we have determined the conditions required for in vitro transcription of the cloned chromosomal crystal gene from bacillus thuringiensis using either the homologous vegetative rna polymerase or a sporulation specific form of this enzyme. the gene is actively transcribed by the latter enzyme (form ii) but not by the vegetative one. evidence for a specific recognition between the form ii enzyme and the promotor site of the crystal gene was obtained by binding experiments. they showed that the bind ...19836306569
[simultaneous reversion for genes thy, dra and drm in bacillus thuringiensis].the reversions of mutations in genes thy, dra (deoxyriboaldolase) and drm (phosphodeoxyribomutase) were studied in bacillus thuringiensis var. galleriae 351. in this strain, both dra and drm mutants are sensitive to the presence of thymidine (tds). in addition to the reversions for one of the properties (thymine requirement or sensitivity to thymidine), the simultaneous reversions for both properties (thy + tdr) were indicated. the frequency of thy + tdr revertants varied from 10(-4) to 10(-8), ...19836303901
increase in osmotic fragility of bovine erythrocytes induced by bacterial phospholipases c.bovine erythrocytes were treated with each of three bacterial phospholipases c; phosphatidylcholine-hydrolyzing phospholipase c (pcase) of clostridium perfringens, sphingomyelinase c (smase) of bacillus cereus and phosphatidylinositol-specific phospholipase c (piase) of bacillus thuringiensis. an increase in osmotic fragility was detected by means of a coil planet centrifugation (cpc) apparatus (biomedical systems co., tokyo) after the treatment with these enzymes. the peak of hemolysis normally ...19836302097
transcriptional and translational start sites for the bacillus thuringiensis crystal protein gene.the nucleotide sequence of the promoter region and part of the coding region of the crystal protein gene from bacillus thuringiensis var. kurstaki hd-1-dipel has been determined by analysis of a recombinant plasmid from escherichia coli. the start points for transcription of the gene in b. thuringiensis and in the e. coli strain carrying the recombinant plasmid were located by s1 nuclease mapping. two adjacent start sites were identified using rnas synthesized during sporulation of b. thuringien ...19836296116
transfer of bacillus thuringiensis plasmids coding for delta-endotoxin among strains of b. thuringiensis and b. cereus.the recently discovered high-frequency transfer of plasmids between strains of bacillus thuringiensis was used to study the genetic relationship between plasmids and production of the insecticidal delta-endotoxin crystal. three strains of b. thuringiensis transmitted the cry+ (crystal-producing) phenotype to cry- (acrystalliferous) b. thuringiensis recipients. agarose gel electrophoresis showed that one specific plasmid from each donor strain was always present in cry+ "transcipients." the size ...19826294667
[plasmids of bacillus thuringiensis var. galleriae strain 612].we studied bacillus thuringiensis var galleriae, strain 612 plasmids. b. thuringiensis cells contain double-stranded plasmid dna molecules (ranging of about 12% from total dna content) with buoyant density 1.59 g/cm3. plasmid dna content was constant during the exponential and stationary phases of bacterial growth. the plasmid fractions consist of dna molecules with molecular weights of 5.9 x 10(6), 10.0 x 10(6), and 110.9 x 10(6) daltons (pvd1, pvd2 pvd3, respectively). endonuclease ecori cuts ...19806252447
[properties of e. coli, salm. typhimurium and cl. perfringens strains isolated from soil containing bacterial insecticides based on bac. thuringiensis]. 19806247250
parasporal crystals produced by oligosporogenous mutans of bacillus thuringiensis (spo-cr+).six oligosporogenic (spo-) mutant strains of bacillus thuringiensis were selected from survivors of treatment with n-methyl-n'-nitro-n-nitrosoguanidine. each strain was blocked at or before stage ii of spore development, but all produced typical bipyramidal-shaped crystalline inclusion bodies. toxicity of the paraporad typical bipyramidal-shaped crystalline inclusion bodies. toxicity of the parasporal endotoxin isolated from the mutant strains was assayed by an in vitro technique using cultured ...19806247047
[the initial activity and persistance of an experimental larvicide with a base of bacillus thuringiensis var. israelensis and a commercial preparation containing the organophosphorus insecticide temephos].the larvicide activity of the experimental preparation san 402 i wdc containing b. thuringiensis var. israelensis and that of abate 500 e (44 % temephos) were tested on the larvae of aedes aegypti. the studies were performed on the following media: distilled water, pure and buffered at ph 9, 7 and 4 and pond water whether or not free from materials in suspension. the activity of both preparations is not influenced by the composition of the media, exception made for the presence of materials in s ...19846208235
[effect of gramicidin d on the exoprotease activity of bacillus thuringiensis].a treatment of bacillus thuringiensis cells with gramicidine d in the medium containing the yeast and polysaccharides increases the lag-phase up to 12 h without a change of the rate during the logarithmic phase of the culture growth. the exoprotease activity of cells treated with gramicidine reaches a maximum value 4 h earlier in comparison with the control culture, and the activity level is increased 2-fold. at a concentration increasing the exoprotease activity, gramicidine was found to induce ...19836196773
toxicity of cyclic peptide antibiotics to larvae of aedes aegypti. 19836196417
[dynamics of nucleic acid and protein content in bacillus thuringiensis cells].the content of dna, rna and protein was assayed in bacillus thuringiensis var. insectus guk at different growth stages and under different cultivation conditions. the content of rna and protein was found to be maximal in the cells at the end of the lag phase and at the beginning of the exponential phase; then, this content decreased with aging of the culture. the content of dna in the strain depended on the conditions of cultivation, and reached amounts corresponding to 10-13 chromosomes when th ...19806156386
field efficacy of bacillus thuringiensis h-14 formulations against mosquito larvae in casuarina & coconut garden pits. 19846151548
oral toxicity of bacillus thuringiensis subsp. israelensis to adult mosquitoes.the solubilized entomotoxin of bacillus thuringiensis subsp. israelensis killed adult male and female mosquitoes of several genera and of various physiological states when it was administered orally. adult mosquito mortality was further influenced when the preparation was contained in sucrose solution. the potential implication for the control of adult mosquitoes is discussed.19846149725
[characteristics of bacillus thuringiensis var. israelensis and its effect on mosquito larvae (diptera: culicidae)]. 19846149708
[use of the 14th serotype of bacillus thuringiensis berl. against the larvae of blood-sucking mosquitoes]. 19846146922
laboratory trials with bacillus thuringiensis serotype h-14 in controlling mosquito larvae. 19846145746
medium for the production of primary powder of bacillus thuringiensis subsp. israelensis.five media, formulated from the seeds of five legume varieties, dried cow blood, and mineral salts, were assessed for the growth and production of insecticidal properties of bacillus thuringiensis subsp. israelensis. bacterial powders prepared from the broth cultures were assayed against the larvae of aedes aegypti, culex quinquefasciatus, and anopheles gambiae. a standard primary powder of b. thuringiensis subsp. israelensis (ips78) was included in the assay for comparison. good growth was obta ...19846144290
[current problems of developing biological methods for controlling the vectors of human parasitic diseases]. 19836143243
bacillus thuringiensis var israelensis: protoxin activation and safety. 19846142364
[effect of amino acids on exoprotease synthesis by bacillus thuringiensis].the influence of certain l-amino acids and their mixtures on the synthesis of exoprotease from bacillus thuringiensis was studied. physiological experiments showed that the mixture of 20 amino acids added to the artificial medium repressed the synthesis of exoprotease. among the compounds studied there are both the compounds which stimulate the synthesis of exoprotease (glutamic and aspartic acids, glycine), and the compounds which repress the synthesis of the enzyme (proline, tryptophane, tyros ...19836139810
the comparative persistence of toxicity of bacillus sphaericus strain 1593 and bacillus thuringiensis serotype h-14 against mosquito larvae in different kinds of environments. 19836139404
bacillus thuringiensis h-14 (vcrc b-17) formulation as mosquito larvicide. 19836134667
effect of bacillus thuringiensis var. israelensis on some egyptian mosquito larvae. 19826130117
comparative bioassays of bacillus thuringiensis h-14 formulations against four species of mosquitoes in malaysia.comparative laboratory bioassays of three formulations of bacillus thuringiensis h-14 (ips-78, san 402-i and bactimos) were conducted against late 3rd/early 4th instar larvae of four species of mosquito, viz., aedes aegypti, culex quinquefasciatus, anopheles balabacensis and mansonia (mansonioides) indiana, in malaysia. from the average response of the mosquito larvae to the three formulations of b. thuringiensis h-14, ae. aegypti was found to be most susceptible, followed by cx. quinquefasciatu ...19826128794
the effect of bacillus thuringiensis var. israelensis on toxorhynchites rutilus rutilus (diptera: culicidae) in the presence and absence of prey. 19826128423
[joint action of bacterial preparations and insect growth regulators on the larvae of blood-sucking mosquitoes]. 19826127605
the action of two formulations containing bacillus thuringiensis (serotype h-14) on some autochthonous mosquito species (diptera : culicidae). 19826126174
[study of the larvicidal activity of bacillus thuringiensis var. israelensis on toxorhynchitinae larvae (author's transl)].toxicity of bacillus thuringiensis var. israelensis, h 14 serotype, has been tested on larvae of toxorhynchites amboinensis, predaceous larvae of other culicinae larvae. the toxorhynchites larvae are not sensitive to the bacterial toxin up to relatively high dosages, except when larvae of aedes aegypti are provided in the suspension. mode of action of the crystal appears to be similar to the one observed in the epithelial cells of intoxicated aedes aegypti larvae midgut.19826125271
field evaluation of the microbial insecticide bacillus thuringiensis serotype h-14 against floodwater mosquitoes.four experimental formulations of the microbial insecticide bacillus thuringiensis var. israelensis (serotype h-14) were evaluated in the field against larvae of the floodwater mosquitoes aedes nigromaculis and psorophora columbiae with ground and aerial application methods. the formulations included abg-6108d (abbott laboratories, north chicago, ill.), a wettable powder containing about 400 iu/mg, sandoz 402-wdc (sadoz, inc., homestead, fla.), a flowable concentrate containing 600 iu/mg, and tw ...19826125127
[results of laboratory trials of bac. thuringiensis berliner preparations on the larvae of blood sucking mosquitoes in the family culicidae. i]. 19826121285
role of the parasporal body in causing toxicity of bacillus thuringiensis toward aedes aegypti larvae. 19826120198
[laboratory and field evaluation of the mosquito larvicidal effect of bacillus thuringiensis var. israelensis (author's transl)]. 19806113102
[evaluation of the larvicidal activity of bacillus thuringiensis serotype h14 on mosquitoes in natural breeding places].the experiments conducted with bacillus thuringiensis var. israelensis, serotype h14, in nature, show that this bacillus has a specific and extremely high larvicidal power against the larval population of culicidae. it operates very quickly but its action seems relatively short-time lasting. considering the increasing resistance of larvae to organophosphorus compounds, this new serotype presents a particular interest for the control of mosquitoes.19806112072
the isolates of bacillus thuringiensis serotype 10 with a highly preferential toxicity to mosquito larvae. 19806108343
[relationship between spore formation and exoproteinase synthesis in bacillus thuringiensis].the localization of exoproteinase in the conventionally "periplasmic" space of bacillus thuringiensis was established. the enzyme located at the outer surface of the membrane was found, in the course of sporogenesis, within the spore where it could fulfill various functions. the role of glutamine synthetase was determined in the regulation of exoproteinase synthesis and sporogenesis. the both processes were inhibited in the conditions suppressing the activity of glutamine synthetase.19806106151
a transposon-like structure related to the delta-endotoxin gene of bacillus thuringiensis.a dna segment (th-sequence) has been found in several strains of bacillus thuringiensis. this th-sequence [3 megadaltons (md)] induces adjacent deletions when it is located in the pam beta 1 plasmid derived from streptococcus faecalis. electron microscopic examination of reannealed single strands of one plasmid (pmt9) carrying such a deletion revealed that the th-sequence corresponds to a single-stranded loop (2.8 md) bounded by a short double-stranded stem (less than 0.2 md). southern blotting ...19846096131
release of alkaline phosphodiesterase i from rat kidney plasma membrane produced by the phosphatidylinositol-specific phospholipase c of bacillus thuringiensis.the release of plasma-membrane-bound enzymes by phosphatidylinositol-specific phospholipase c obtained from bacillus thuringiensis was investigated. among the ectoenzymes of plasma membrane tested, alkaline phosphodiesterase i was released markedly from rat kidney cortex slices, in addition to alkaline phosphatase and 5'-nucleotidase. other membrane-bound enzymes; alanine aminopeptidase, leucine aminopeptidase, dipeptidyl peptidase, leucine aminopeptidase, dipeptidyl peptidase iv, esterase and g ...19846096028
[molecular genetic studies of the dna promotor regions in bacillus thuringiensis subsp. galleriae 69-6. ii. increased level of gene expression resulting from is1 element integration].the 1.45 kb promoter containing hindiii fragment of bacillus thuringiensis dna promotes the expression of the tet gene of recombinant ppbt9 plasmid in escherichia coli cells. spontaneous mutants of this plasmid were isolated and analysed. they are responsible for an increase in the level of tetracycline resistance. this 3-fold increase resulted from integration of is1 element into the bacillar promoter containing hindiii fragment, which led to formation of a mutant ppbt9::is1 plasmid. the is seq ...19846094308
inverted repeat sequences flank a bacillus thuringiensis crystal protein gene.two sets of inverted repeat dna sequences, ir2150 and ir1750, were discovered flanking the crystal protein gene on the 75-kilobase plasmid of bacillus thuringiensis subsp. kurstaki hd73. a restriction map of ca. 40 kilobases around the crystal protein gene was constructed, and the positions of the copies of ir2150 and ir1750 were determined. three copies of ir2150 were found flanking the crystal protein gene in an inverted orientation, and one partial and three intact copies of ir1750 were found ...19846090438
cloning and expression in escherichia coli of the insecticidal delta-endotoxin gene of bacillus thuringiensis var. israelensis.recombinant plasmids containing the mosquitocidal delta-endotoxin gene were constructed by inserting hindiii fragments of the bacillus thuringiensis var. israelensis 72-75 md plasmid in to the escherichia coli vector puc12. two recombinants producing the 26 000 da delta-endotoxin (pip173 and pip174) were identified by screening clones in an e. coli in vitro transcription-translation system. both recombinants were 12.4 kb chimaeric plasmids comprising puc12 and a common 9.7 kb hindiii fragment of ...19846090216
effects of valine, leucine, and isoleucine on the growth of bacillus thuringiensis and related bacteria. 19676083260
the toxic action of bacillus thuringiensis 'exotoxin' on drosophila reared in yeast-containing and yeast-free media. 19676076335
antigenic analysis of the digests of the crystal toxins of bacillus thuringiensis. 19676073995
effect of heat-stable bacillus thuringiensis toxin on armyworms. 19676073678
effect of valine, leucine, and isoleucine on the production of fly toxin by bacillus thuringiensis and related organisms. 19676073674
the dissociation of bacillus thuringiensis. 19676064047
electron microscopy of the crystal shape and structure of two strains of bacillus thuringiensis berliner. 19676043165
biphasic system for separation of spores and crystals of bacillus thuringiensis. 19676039366
mesosomes in m-tyrosine-inhibited bacillus thuringiensis. 19676025420
[pigment producing variants of bacillus thuringiensis berliner isolated from the silk worm]. 19666002934
an electron microscope study of protein crystals in different strains of the bacillus thuringiensis group. 19665984731
four new bacteriophages of bacillus thuringiensis. 19665980917
the production of heat-stable toxin by nine serotypes of bacillus thuringiensis. 19665980201
preliminary report on the larval development of the horn fly, haematobia irritans, in feces from cattle given fractions of a commercial preparation of bacillus thuringiensis. 19665944662
inactivation by irradiation of spores of bacillus thuringiensis var. thuringiensis. 19665939388
on the chemical nature of the heat-stable exotoxin of bacillus thuringiensis. 19665927971
studies on the structure of parasporal inclusions from bacillus thuringiensis. 19655880865
bacteriostatic and bacteriocidal effects of extracts of foliage from various plant species on bacillus thuringiensis var. thuringiensis berliner. 19655829857
the effectiveness of the heat-stable toxin of bacillus thuringiensis var. thuringiensis berliner on larvae of musca domestica linnaeus. 19655827560
longevity of bacillus thuringiensis berliner in the rumen. 19655827558
serological studies of the protein parasporal inclusions of bacillus thuringiensis var. sotto ishiwata. 19655827554
the ecology of serotype 4b of bacillus thuringiensis. 19695823663
a purification and some properties of an insecticidal exotoxin from bacillus thuringiensis berliner.an insecticidal exotoxin from bacillus thuringiensis var. thuringiensis (berliner) has been purified. the efficiency of each stage of the purification has been ascertained and the yield of toxic material estimated by means of a quantitative bioassay. it is shown that the exotoxin is an adenine derivative substituted at position 9 and having a molecular weight of approximately 825. it can be dephosphorylated enzymically or chemically under conditions that define the exotoxin as a phosphomonoester ...19695820635
some chemical and biological studies on an exotoxin from bacillus thuringiensis var. thuringiensis berliner. 19695810059
[insecticides from bacillus thuringiensis]. 19695788357
crystalline inclusions in bacillus thuringiensis.crystalline inclusion bodies resembling those seen in clostridium cochlearium were detected in cultures of bacillus thuringiensis infected with bacteriophage.19695784229
susceptibility of several forest insects of british columbia to commercially produced bacillus thuringiensis. ii. laboratory and field pathogenicity tests. 19695777834
ecological significance of antibiotics of some varieties of bacillus thuringiensis. 19695777828
oral and parenteral toxicity of bacillus thuringiensis 'exotoxin', and its inactivation in larvae of galleria mellonella. 19695766603
[the occurrence of different varieties of bacillus thuringiensis in germany]. 19685745201
biochemical homology between crystal and spore protein of bacillus thuringiensis.the crystalline inclusion of bacillus thuringiensis, dissolved in 8 m urea containing 10% 2-mercaptoethanol and dialyzed to ph 8.3 to 8.5, was compared with a fraction obtained by the same extraction procedure from spores broken by dry rupture. the two fractions behaved similarly on chromatography with sephadex g-100 and diethylaminoethyl cellulose. the preparations behaved identically on acrylamide gel electrophoresis at ph 12 and ph 9.5. further, peptide maps of the two fractions obtained afte ...19685732505
isolation and characterization of three autolytic enzymes associated with sporulation of bacillus thuringiensis var. thuringiensis.cells of bacillus thuringiensis containing refractile spores autolyzed readily when suspended in buffer. the autolysate contained enzymes which lysed vegetative cell walls of the organism. three enzymes were isolated from the autolysate, and each was purified approximately 30-fold. one enzyme, most active near ph 4.0, was found to be an n-acetylmuramidase. the other two enzymes exhibited ph optima at 8.5. one was stimulated by cobalt ions and the other was not. the cobalt-stimulated enzyme was s ...19685732500
inhibition of dna-dependent rna polymerase by the exotosin of bacillus thuringiensis var. gelechiae. 19685727141
a classification of strains of bacillus thuringiensis berliner with a key to their differentiation. 19685725005
induction of resistance to bacillus thuringiensis in a laboratory strain of house flies. 19685723773
bacteriophage of bacillus thuringiensis berliner. 19685715033
control of the greater and lesser wax moths (galleria mellonella and achroia grisella) with bacillus thuringiensis. 19685672009
chemical composition of the cell wall of bacillus thuringiensis var. thuringiensis. 19685640393
[effect of growth conditions on biological traits of some bacillus thuringiensis cultures]. 19675618183
isolation of a water-soluble toxin from a commercial microbial insecticide based on bacillus thuringiensis. 19715575753
use of cryptograms for characterization of strains of the bacillus thuringiensis-bacillus cereus group. 19715575752
sensitivity of bacillus thuringiensis var. thuringiensis to various insecticides and herbicides. 19715575750
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