Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| the pseudomonas syringae type iii effector hopam1 enhances virulence on water-stressed plants. | pseudomonas syringae strains deliver diverse type iii effector proteins into host cells, where they can act as virulence factors. although the functions of the majority of type iii effectors are unknown, several have been shown to interfere with plant basal defense mechanisms. type iii effectors also could contribute to bacterial virulence by enhancing nutrient uptake and pathogen adaptation to the environment of the host plant. we demonstrate that the type iii effector hopam1 (formerly known as ... | 2008 | 18257685 |
| characterization of antimicrobial compounds produced by pseudomonas aurantiaca s-1. | pseudomonas aurantiaca s-1 can serve as a natural source of pesticides towards phytopathogens like fusarium oxysporum p1 and pseudomonas syringae pv. glycinea bim b-280. the largest pool of produced antimicrobial compounds was found in four days-old spent culture supernatant. at least two groups of bioactive substances were identified, one responsible for the antibacterial activity and the other for the antifungal activity. the fraction with strong antibacterial activity had the molecular mass 2 ... | 2007 | 18254494 |
| comparative bacterial proteomics: analysis of the core genome concept. | while comparative bacterial genomic studies commonly predict a set of genes indicative of common ancestry, experimental validation of the existence of this core genome requires extensive measurement and is typically not undertaken. enabled by an extensive proteome database developed over six years, we have experimentally verified the expression of proteins predicted from genomic ortholog comparisons among 17 environmental and pathogenic bacteria. more exclusive relationships were observed among ... | 2008 | 18253490 |
| bacterial non-host resistance: interactions of arabidopsis with non-adapted pseudomonas syringae strains. | although interactions of plants with virulent and avirulent host pathogens are under intensive study, relatively little is known about plant interactions with non-adapted pathogens and the molecular events underlying non-host resistance. here we show that two pseudomonas syringae strains for which arabidopsis is a non-host plant, p. syringae pathovar (pv.) glycinea (psg) and p. syringae pv. phaseolicola (psp),induce salicylic acid (sa) accumulation and pathogenesis-related gene expression at ino ... | 2007 | 18251883 |
| a high-throughput chemical screen for resistance to pseudomonas syringae in arabidopsis. | the study of plant pathogenesis and the development of effective treatments to protect plants from diseases could be greatly facilitated by a high-throughput pathosystem to evaluate small-molecule libraries for inhibitors of pathogen virulence. the interaction between the gram-negative bacterium pseudomonas syringae and arabidopsis thaliana is a model for plant pathogenesis. however, a robust high-throughput assay to score the outcome of this interaction is currently lacking. we demonstrate that ... | 2008 | 18248597 |
| auxotrophy in natural isolate: minimal requirements for growth of the antarctic psychrotrophic bacterium pseudomonas syringae lz4w. | a chemically defined minimal medium has been developed for growing the antarctic psychrotrophic bacterium pseudomonas syringae lz4w, a model system for studying cold adaptation. this natural isolate from antarctica has an absolute requirement for two branched chain amino acids, isoleucine and valine, in addition to low osmolality of the growth medium. the bacterium contains threonine deaminase but lacks acetohydroxyacid synthase suggesting that a defect lies in the isoleucine and valine biosynth ... | 2008 | 18247394 |
| nmr structure of the peptidyl-trna hydrolase domain from pseudomonas syringae expands the structural coverage of the hydrolysis domains of class 1 peptide chain release factors. | 2008 | 18247350 | |
| characteristic expression of twelve rice pr1 family genes in response to pathogen infection, wounding, and defense-related signal compounds (121/180). | pathogenesis-related (pr) proteins have been used as markers of plant defense responses, and are classified into 17 families. however, precise information on the majority members in specific pr families is still limited. we were interested in the individual characteristics of rice pr1 family genes, and selected 12 putatively active genes using rice genome databases for expressed genes. all were upregulated upon compatible and/or incompatible rice-blast fungus interactions; three were upregulated ... | 2008 | 18247056 |
| hypericum perforatum plant cells reduce agrobacterium viability during co-cultivation. | plant recalcitrance is the major barrier in developing agrobacterium-mediated transformation protocols for several important plant species. despite the substantial knowledge of t-dna transfer process, very little is known about the factors leading to the plant recalcitrance. here, we analyzed the basis of hypericum perforatum l. (hp) recalcitrance to agrobacterium-mediated transformation using cell suspension culture. when challenged with agrobacterium, hp cells swiftly produced an intense oxida ... | 2008 | 18247048 |
| tomato transcriptional changes in response to clavibacter michiganensis subsp. michiganensis reveal a role for ethylene in disease development. | clavibacter michiganensis subsp. michiganensis (cmm) is a gram-positive actinomycete, causing bacterial wilt and canker disease in tomato (solanum lycopersicum). host responses to gram-positive bacteria and molecular mechanisms associated with the development of disease symptoms caused by cmm in tomato are largely unexplored. to investigate plant responses activated during this compatible interaction, we used microarray analysis to monitor changes in host gene expression during disease developme ... | 2008 | 18245454 |
| chemical and antibacterial constituents of skimmia anquetelia. | investigation of the leaves of skimmia anquetelia (rutaceae) led to the isolation of a new coumarin glucoside 7,8-dihdroxy-6-[3'-beta- d-glucopyranosyloxy-2'(xi)-hydroxy-3'-methylbutyl]-coumarin ( 1) together with five known coumarins: 6-(2,3-dihydroxy-3-methylbutyl)-7-methoxycoumarin ( 2), skimmin ( 3), osthol ( 4), esculetin ( 5) and scopuletin ( 6). the antibacterial activity of compounds 1 and 3 was also investigated against the plant bacterial pathogens agrobacterium tumifaciens, pseudomona ... | 2008 | 18240101 |
| functional role of the additional domains in inulosucrase (isla) from leuconostoc citreum cw28. | inulosucrase (isla) from leuconostoc citreum cw28 belongs to a new subfamily of multidomain fructosyltransferases (ftfs), containing additional domains from glucosyltransferases. it is not known what the function of the additional domains in this subfamily is. | 2008 | 18237396 |
| analysis of non-tir nbs-lrr resistance gene analogs in musa acuminata colla: isolation, rflp marker development, and physical mapping. | many commercial banana varieties lack sources of resistance to pests and diseases, as a consequence of sterility and narrow genetic background. fertile wild relatives, by contrast, possess greater variability and represent potential sources of disease resistance genes (r-genes). the largest known family of plant r-genes encode proteins with nucleotide-binding site (nbs) and c-terminal leucine-rich repeat (lrr) domains. conserved motifs in such genes in diverse plant species offer a means for iso ... | 2008 | 18234103 |
| the alternative sigma factor algt, but not alginate synthesis, promotes in planta multiplication of pseudomonas syringae pv. glycinea. | the phytopathogen pseudomonas syringae pv. glycinea produces the exopolysaccharide (eps) alginate, which is thought to function in epiphytic fitness and virulence. a key regulator for alginate biosynthesis in pseudomonas aeruginosa and p. syringae is the alternative sigma factor algt (sigma(22)). in this study, the contribution of alginate synthesis and algt to in planta epiphytic fitness and virulence of p. syringae was examined. alginate biosynthesis mutants were generated for the p. syringae ... | 2008 | 18227245 |
| overexpression of atsgt1, an arabidopsis salicylic acid glucosyltransferase, leads to increased susceptibility to pseudomonas syringae. | we reported previously that a recombinant salicylic acid (sa) glucosyltransferase1 (atsgt1) from arabidopsis thaliana catalyzes the formation of both sa 2-o-beta-d-glucoside (sag) and the glucose ester of sa (sge). here, transgenic arabidopsis plants overexpressing atsgt1 have been constructed, and their phenotypes analyzed. compared to wild-type plants, transgenic plants showed an increased susceptibility to pseudomonas syringae and reduced the accumulation levels of both free sa and its glucos ... | 2008 | 18226820 |
| modulation of defense signal transduction by flagellin-induced wrky41 transcription factor in arabidopsis thaliana. | flagellin, a component of the flagellar filament of pseudomonas syringae pv. tabaci 6605 (pta), induces hypersensitive reaction in its non-host arabidopsis thaliana. we identified the wrky41 gene, which belongs to a multigene family encoding wrky plant-specific transcription factors, as one of the flagellin-inducible genes in a. thaliana. expression of wrky41 is induced by inoculation with the incompatible pathogen p. syringae pv. tomato dc3000 (pto) possessing avrrpt2 and the non-host pathogens ... | 2008 | 18219494 |
| genome-wide expression profiling arabidopsis at the stage of golovinomyces cichoracearum haustorium formation. | compatibility between plants and obligate biotrophic fungi requires fungal mechanisms for efficiently obtaining nutrients and counteracting plant defenses under conditions that are expected to induce changes in the host transcriptome. a key step in the proliferation of biotrophic fungi is haustorium differentiation. here we analyzed global gene expression patterns in arabidopsis thaliana leaves during the formation of haustoria by golovinomyces cichoracearum. at this time, the endogenous levels ... | 2008 | 18218973 |
| myb72 is required in early signaling steps of rhizobacteria-induced systemic resistance in arabidopsis. | colonization of arabidopsis thaliana roots by nonpathogenic pseudomonas fluorescens wcs417r bacteria triggers a jasmonate/ethylene-dependent induced systemic resistance (isr) that is effective against a broad range of pathogens. microarray analysis revealed that the r2r3-myb-like transcription factor gene myb72 is specifically activated in the roots upon colonization by wcs417r. here, we show that t-dna knockout mutants myb72-1 and myb72-2 are incapable of mounting isr against the pathogens pseu ... | 2008 | 18218967 |
| nonribosomal peptide synthetases involved in the production of medically relevant natural products. | natural products biosynthesized wholly or in part by nonribosomal peptide synthetases (nrpss) are some of the most important drugs currently used clinically for the treatment of a variety of diseases. since the initial research into nrpss in the early 1960s, we have gained considerable insights into the mechanism by which these enzymes assemble these natural products. this review will present a brief history of how the basic mechanistic steps of nrpss were initially deciphered and how this infor ... | 2008 | 18217713 |
| [antimicrobial activity of calendula l. plants]. | the sap of different organs of genus calendula plant species has been studied for antimicrobial activity. the sap of racemes demonstrated the most expressed antimicrobial effect while that of the roots - the least one. calendula species inhibited all tested pathogenic microorganisms, especially pseudomonas syringae, p. fluorescens, xanthomonas campestris, agrobacterium tumefaciens. calendula suffruticosa was the most active to all investigated microorganisms. | 2007 | 18217350 |
| the evolution of gene collectives: how natural selection drives chemical innovation. | dna sequencing has become central to the study of evolution. comparing the sequences of individual genes from a variety of organisms has revolutionized our understanding of how single genes evolve, but the challenge of analyzing polygenic phenotypes has complicated efforts to study how genes evolve when they are part of a group that functions collectively. we suggest that biosynthetic gene clusters from microbes are ideal candidates for the evolutionary study of gene collectives; these selfish g ... | 2008 | 18216259 |
| quantitative 1h nmr metabolomics reveals extensive metabolic reprogramming of primary and secondary metabolism in elicitor-treated opium poppy cell cultures. | opium poppy (papaver somniferum) produces a diverse array of bioactive benzylisoquinoline alkaloids and has emerged as a model system to study plant alkaloid metabolism. the plant is cultivated as the only commercial source of the narcotic analgesics morphine and codeine, but also produces many other alkaloids including the antimicrobial agent sanguinarine. modulations in plant secondary metabolism as a result of environmental perturbations are often associated with the altered regulation of oth ... | 2008 | 18211706 |
| optimized cdna libraries for virus-induced gene silencing (vigs) using tobacco rattle virus. | abstract: | 2008 | 18211705 |
| bacterial signaling and motility: sure bets. | 2008 | 18203824 | |
| recognition of the hyaloperonospora parasitica effector atr13 triggers resistance against oomycete, bacterial, and viral pathogens. | phytopathogenic oomycetes cause some of the most devastating diseases affecting agricultural crops. hyaloperonospora parasitica is a native oomycete pathogen of arabidopsis and is related to other oomycete phytopathogens that include several species of phytophthora, including the causal agent of potato late blight. recently, four oomycete effector genes have been isolated, and several oomycete genomes have been sequenced. we have developed an efficient and genetically amenable system to test put ... | 2008 | 18198274 |
| small rnas and the regulation of cis-natural antisense transcripts in arabidopsis. | in spite of large intergenic spaces in plant and animal genomes, 7% to 30% of genes in the genomes encode overlapping cis-natural antisense transcripts (cis-nats). the widespread occurrence of cis-nats suggests an evolutionary advantage for this type of genomic arrangement. experimental evidence for the regulation of two cis-nat gene pairs by natural antisense transcripts-generated small interfering rnas (nat-sirnas) via the rna interference (rnai) pathway has been reported in arabidopsis. howev ... | 2008 | 18194570 |
| activation of the indole-3-acetic acid-amido synthetase gh3-8 suppresses expansin expression and promotes salicylate- and jasmonate-independent basal immunity in rice. | new evidence suggests a role for the plant growth hormone auxin in pathogenesis and disease resistance. bacterial infection induces the accumulation of indole-3-acetic acid (iaa), the major type of auxin, in rice (oryza sativa). iaa induces the expression of expansins, proteins that loosen the cell wall. loosening the cell wall is key for plant growth but may also make the plant vulnerable to biotic intruders. here, we report that rice gh3-8, an auxin-responsive gene functioning in auxin-depende ... | 2008 | 18192436 |
| lipase expression in pseudomonas alcaligenes is under the control of a two-component regulatory system. | preliminary observations in a large-scale fermentation process suggested that the lipase expression of pseudomonas alcaligenes can be switched on by the addition of certain medium components, such as soybean oil. in an attempt to elucidate the mechanism of induction of lipase expression, we have set up a search method for genes controlling lipase expression by use of a cosmid library containing fragments of p. alcaligenes genomic dna. a screen for lipase hyperproduction resulted in the selection ... | 2008 | 18192420 |
| crt1, an arabidopsis atpase that interacts with diverse resistance proteins and modulates disease resistance to turnip crinkle virus. | plant immunity frequently involves the recognition of pathogen-encoded avirulence (avr) factors by their corresponding plant resistance (r) proteins. this triggers the hypersensitive response (hr) where necrotic lesions formed at the site(s) of infection help restrict pathogen spread. hrt is an arabidopsis r protein required for resistance to turnip crinkle virus (tcv). in a genetic screen for mutants compromised in the recognition of tcv's avr factor, we identified crt1 (compromised recognition ... | 2008 | 18191794 |
| assaying protein palmitoylation in plants. | protein s-acylation (also known as palmitoylation) is the reversible post-translational addition of acyl lipids to cysteine residues in proteins through a thioester bond. it allows strong association with membranes. whilst prediction methods for s-acylation exist, prediction is imperfect. existing protocols for demonstrating the s-acylation of plant proteins are either laborious and time consuming or expensive. | 2008 | 18190696 |
| the life history of the plant pathogen pseudomonas syringae is linked to the water cycle. | pseudomonas syringae is a plant pathogen well known for its capacity to grow epiphytically on diverse plants and for its ice-nucleation activity. the ensemble of its known biology and ecology led us to postulate that this bacterium is also present in non-agricultural habitats, particularly those associated with water. here, we report the abundance of p. syringae in rain, snow, alpine streams and lakes and in wild plants, in addition to the previously reported abundance in epilithic biofilms. eac ... | 2008 | 18185595 |
| pseudomonas syringae pv. tomato dc3000 uses constitutive and apoplast-induced nutrient assimilation pathways to catabolize nutrients that are abundant in the tomato apoplast. | the plant apoplast is the intercellular space that surrounds plant cells, in which metabolic and physiological processes relating to cell wall biosynthesis, nutrient transport, and stress responses occur. the apoplast is also the primary site of infection for hemibiotrophic pathogens such as p. syringae, which obtain nutrients directly from apoplastic fluid. we have used apoplastic fluid extracted from healthy tomato leaves as a growth medium for pseudomonas spp. in order to investigate the role ... | 2008 | 18184070 |
| simultaneous interaction of arabidopsis thaliana with bradyrhizobium sp. strain ors278 and pseudomonas syringae pv. tomato dc3000 leads to complex transcriptome changes. | induced systemic resistance (isr) is a process elicited by telluric microbes, referred to as plant growth-promoting rhizobacteria (pgpr), that protect the host plant against pathogen attacks. isr has been defined from studies using pseudomonas strains as the biocontrol agent. here, we show for the first time that a photosynthetic bradyrhizobium sp. strain, ors278, also exhibits the ability to promote isr in arabidopsis thaliana, indicating that the isr effect may be a widespread ability. to inve ... | 2008 | 18184068 |
| expression of a ring-hc protein from rice improves resistance to pseudomonas syringae pv. tomato dc3000 in transgenic arabidopsis thaliana. | a cdna clone (osrhc1) was obtained, which encodes a novel ring zinc finger protein sharing similar structural features (multiple transmembrane domains at the n-half; a unique ring zinc finger consensus cys-x(2)-cys-x(11)-cys-x-his-x(3)-cys-x(2)-cys-x(6)-cys-x(2)-cys at the c terminus) to a group of closely related annotated proteins from both monocots and dicots. osrhc1 was found to be localized on plasma membrane of rice cells and induced by wounding in rice lines containing xa loci. ecotopic e ... | 2007 | 18182423 |
| comparative expression analysis of genes induced during development of bacterial rot and induction of hypersensitive cell death in lettuce. | the development of bacterial rot disease caused by pseudomonas cichorii is closely associated with programmed cell death. to investigate the molecular events occurring during the development of bacterial rot, we isolated 20 p. cichorii-responsive genes (pcrgs) in lettuce by differential display. among these pcrgs, signal transduction-, transcription/translation- and defense/stress responses-related pcrgs were subjected to a comparative expression study. we used rna samples isolated from lettuce ... | 2008 | 18171591 |
| comparative genomic analyses of copper transporters and cuproproteomes reveal evolutionary dynamics of copper utilization and its link to oxygen. | copper is an essential trace element in many organisms and is utilized in all domains of life. it is often used as a cofactor of redox proteins, but is also a toxic metal ion. intracellular copper must be carefully handled to prevent the formation of reactive oxygen species which pose a threat to dna, lipids, and proteins. in this work, we examined patterns of copper utilization in prokaryotes by analyzing the occurrence of copper transporters and copper-containing proteins. many organisms, incl ... | 2008 | 18167539 |
| detection of plant-modulated alterations in antifungal gene expression in pseudomonas fluorescens cha0 on roots by flow cytometry. | the biocontrol activity of the root-colonizing pseudomonas fluorescens strain cha0 is largely determined by the production of antifungal metabolites, especially 2,4-diacetylphloroglucinol. the expression of these metabolites depends on abiotic and biotic environmental factors, in particular, elements present in the rhizosphere. in this study, we have developed a new method for the in situ analysis of antifungal gene expression using flow cytometry combined with green fluorescent protein (gfp)-ba ... | 2008 | 18165366 |
| detection of plant-modulated alterations in antifungal gene expression in pseudomonas fluorescens cha0 on roots by flow cytometry. | the biocontrol activity of the root-colonizing pseudomonas fluorescens strain cha0 is largely determined by the production of antifungal metabolites, especially 2,4-diacetylphloroglucinol. the expression of these metabolites depends on abiotic and biotic environmental factors, in particular, elements present in the rhizosphere. in this study, we have developed a new method for the in situ analysis of antifungal gene expression using flow cytometry combined with green fluorescent protein (gfp)-ba ... | 2008 | 18165366 |
| the downy mildew effector proteins atr1 and atr13 promote disease susceptibility in arabidopsis thaliana. | the downy mildew (hyaloperonospora parasitica) effector proteins atr1 and atr13 trigger rpp1-nd/wsb- and rpp13-nd-dependent resistance, respectively, in arabidopsis thaliana. to better understand the functions of these effectors during compatible and incompatible interactions of h. parasitica isolates on arabidopsis accessions, we developed a novel delivery system using pseudomonas syringae type iii secretion via fusions of atrs to the n terminus of the p. syringae effector protein, avrrps4. atr ... | 2007 | 18165328 |
| s-nitrosylation of peroxiredoxin ii e promotes peroxynitrite-mediated tyrosine nitration. | nitric oxide (no) is a free radical product of cell metabolism that plays diverse and important roles in the regulation of cellular function. s-nitrosylation is emerging as a specific and fundamental posttranslational protein modification for the transduction of no bioactivity, but very little is known about its physiological functions in plants. we investigated the molecular mechanism for s-nitrosylation of peroxiredoxin ii e (prxii e) from arabidopsis thaliana and found that this posttranslati ... | 2007 | 18165327 |
| the jaz proteins link jasmonate perception with transcriptional changes. | 2007 | 18165326 | |
| parasitism by cuscuta pentagona attenuates host plant defenses against insect herbivores. | considerable research has examined plant responses to concurrent attack by herbivores and pathogens, but the effects of attack by parasitic plants, another important class of plant-feeding organisms, on plant defenses against other enemies has not been explored. we investigated how attack by the parasitic plant cuscuta pentagona impacted tomato (solanum lycopersicum) defenses against the chewing insect beet armyworm (spodoptera exigua; baw). in response to insect feeding, c. pentagona-infested ( ... | 2008 | 18165323 |
| pseudomonas 2007. | 2008 | 18165299 | |
| pseudomonas 2007. | 2008 | 18165299 | |
| how novel methods can help discover more information about foodborne pathogens. | considerable emphasis is being placed on quantitative risk assessment modelling as a basis for regulation of trade in food products. however, for models to be accurate, information about the behaviour of potential pathogens in foods needs to be available. the question is how to obtain this knowledge in a simple and cost effective way. one technique that has great potential is the use of reporter bacteria which have been genetically modified to express a phenotype that can be easily monitored, su ... | 2000 | 18159282 |
| the complete genome sequence and analysis of the epsilonproteobacterium arcobacter butzleri. | arcobacter butzleri is a member of the epsilon subdivision of the proteobacteria and a close taxonomic relative of established pathogens, such as campylobacter jejuni and helicobacter pylori. here we present the complete genome sequence of the human clinical isolate, a. butzleri strain rm4018. | 2007 | 18159241 |
| pseudomonas syringae effector avrpto blocks innate immunity by targeting receptor kinases. | plants use receptor kinases, such as fls2 and efr, to perceive bacterial pathogens and initiate innate immunity. this immunity is often suppressed by bacterial effectors, allowing pathogen propagation. to counteract, plants have evolved disease resistance genes that detect the bacterial effectors and reinstate resistance. the pseudomonas syringae effector avrpto promotes infection in susceptible plants but triggers resistance in plants carrying the protein kinase pto and the associated resistanc ... | 2008 | 18158241 |
| salicylic acid and systemic acquired resistance play a role in attenuating crown gall disease caused by agrobacterium tumefaciens. | we investigated the effects of salicylic acid (sa) and systemic acquired resistance (sar) on crown gall disease caused by agrobacterium tumefaciens. nicotiana benthamiana plants treated with sa showed decreased susceptibility to agrobacterium infection. exogenous application of sa to agrobacterium cultures decreased its growth, virulence, and attachment to plant cells. using agrobacterium whole-genome microarrays, we characterized the direct effects of sa on bacterial gene expression and showed ... | 2008 | 18156296 |
| pseudomonas syringae bett is a low-affinity choline transporter that is responsible for superior osmoprotection by choline over glycine betaine. | the plant pathogen pseudomonas syringae derives better osmoprotection from choline than from glycine betaine, unlike most bacteria that have been characterized. in this report, we identified a betaine/carnitine/choline family transporter (bcct) in p. syringae pv. tomato strain dc3000 that mediates the transport of choline and acetylcholine. this transporter has a particularly low affinity (k(m) of 876 microm) and high capacity (v(max) of 80 nmol/min/mg of protein) for choline transport relative ... | 2008 | 18156257 |
| pseudomonas syringae bett is a low-affinity choline transporter that is responsible for superior osmoprotection by choline over glycine betaine. | the plant pathogen pseudomonas syringae derives better osmoprotection from choline than from glycine betaine, unlike most bacteria that have been characterized. in this report, we identified a betaine/carnitine/choline family transporter (bcct) in p. syringae pv. tomato strain dc3000 that mediates the transport of choline and acetylcholine. this transporter has a particularly low affinity (k(m) of 876 microm) and high capacity (v(max) of 80 nmol/min/mg of protein) for choline transport relative ... | 2008 | 18156257 |
| systemic and intracellular responses to photooxidative stress in arabidopsis. | as the sun tracks daily through the sky from east to west, different parts of the canopy are exposed to high light (hl). the extent of and mechanisms by which a systemic acquired acclimation (saa) response might preacclimate shaded leaves that will be subsequently exposed to full sunlight is largely undefined. we investigated the role of an arabidopsis thaliana zinc finger transcription factor, zat10, in saa. zat10 overexpression resulted in enhanced tolerance to photoinhibitory light and exogen ... | 2007 | 18156220 |
| rar1 and hsp90 form a complex with rac/rop gtpase and function in innate-immune responses in rice. | a rice (oryza sativa) rac/rop gtpase, os rac1, is involved in innate immunity, but its molecular function is largely unknown. rar1 (for required for mla12 resistance) and hsp90 (a heat shock protein 90 kd) are important components of r gene-mediated disease resistance, and their function is conserved in several plant species. hsp90 has also recently been shown to be important in mammalian innate immunity. however, their functions at the molecular level are not well understood. in this study, we ... | 2007 | 18156216 |
| the biochemistry and biology of extracellular plant lipid-transfer proteins (ltps). | plant lipid-transfer proteins (ltps) are abundant, small, lipid binding proteins that are capable of exchanging lipids between membranes in vitro. despite their name, a role in intracellular lipid transport is considered unlikely, based on their extracellular localization. a number of other biological roles, including antimicrobial defense, signaling, and cell wall loosening, have been proposed, but conclusive evidence is generally lacking, and these functions are not well correlated with in vit ... | 2008 | 18096636 |
| specific elements of the glyoxylate pathway play a significant role in the functional transition of the soybean cotyledon during seedling development. | the soybean (glycine max) cotyledon is a specialized tissue whose main function is to serve as a nutrient reserve that supplies the needs of the young plant throughout seedling development. during this process the cotyledons experience a functional transition to a mainly photosynthetic tissue. to identify at the genetic level the specific active elements that participate in the natural transition of the cotyledon from storage to photosynthetic activity, we studied the transcript abundance profil ... | 2007 | 18093333 |
| three-dimensional models of nb-arc domains of disease resistance proteins in tomato, arabidopsis, and flax. | three dimensional models of nb-arc domains in five different proteins were constructed based on the recently published crystal structure of the apoptotic protease activating factor 1, of which two are for tomato species, one each for flax, arabidopsis, and nematode. standard multiple sequence alignment was performed for chosen members of the nb-arc domains, very divergent from each other in protein sequence, followed by homology model building and structure refinement. in this alignment, amino a ... | 2008 | 18092830 |
| interplay between ja, sa and aba signalling during basal and induced resistance against pseudomonas syringae and alternaria brassicicola. | we have examined the role of the callose synthase pmr4 in basal resistance and beta-aminobutyric acid-induced resistance (baba-ir) of arabidopsis thaliana against the hemi-biotrophic pathogen pseudomonas syringae and the necrotrophic pathogen alternaria brassicicola. compared to wild-type plants, the pmr4-1 mutant displayed enhanced basal resistance against p. syringae, which correlated with constitutive expression of the pr-1 gene. treating the pmr4-1 mutant with baba boosted the already elevat ... | 2008 | 18088307 |
| plastid omega3-fatty acid desaturase-dependent accumulation of a systemic acquired resistance inducing activity in petiole exudates of arabidopsis thaliana is independent of jasmonic acid. | systemic acquired resistance (sar) is an inducible defense mechanism that is activated throughout the plant, subsequent to localized inoculation with a pathogen. the establishment of sar requires translocation of an unknown signal from the pathogen-inoculated leaf to the distal organs, where salicylic acid-dependent defenses are activated. we demonstrate here that petiole exudates (pexs) collected from arabidopsis leaves inoculated with an avirulent (avr) pseudomonas syringae strain promote resi ... | 2008 | 18088304 |
| fuscopeptins, antimicrobial lipodepsipeptides from pseudomonas fuscovaginae, are channel forming peptides active on biological and model membranes. | fp-a and fp-b are ldps produced by the plant pathogen pseudomonas fuscovaginae. as expected from their primary structure, they shared a similar mechanism of action with the better characterized sps, synthesized by strains of pseudomonas syringae pv. syringae. indeed, they displayed hemolytic activity on human erythrocytes and were able to induce calcein release from luvs: the effect was dependent on the concentration of the fps and the lipid composition of the liposome and, in particular, it inc ... | 2008 | 18085513 |
| natural variation in rps2-mediated resistance among arabidopsis accessions: correlation between gene expression profiles and phenotypic responses. | natural variation in gene expression (expression traits or e-traits) is increasingly used for the discovery of genes controlling traits. an important question is whether a particular e-trait is correlated with a phenotypic trait. here, we examined the correlations between phenotypic traits and e-traits among 10 arabidopsis thaliana accessions. we studied defense against pseudomonas syringae pv tomato dc3000 (pst), with a focus on resistance gene-mediated resistance triggered by the type iii effe ... | 2007 | 18083910 |
| the pseudomonas aeruginosa type iii secretion system plays a dual role in the regulation of caspase-1 mediated il-1beta maturation. | pseudomonas aeruginosa is an opportunistic bacterial pathogen that forms a serious problem for immunocompromised patients and also the leading cause of mortality in cystic fibrosis. the overall importance of a functional type iii secretion system (t3ss) in p. aeruginosa virulence has been well established, but the underlying mechanisms are still unclear. using in vitro infected macrophages as w as a murine model of acute lung infection, we show that the caspase-1 mediated maturation and secretio ... | 2008 | 18081695 |
| the pseudomonas aeruginosa type iii secretion system plays a dual role in the regulation of caspase-1 mediated il-1beta maturation. | pseudomonas aeruginosa is an opportunistic bacterial pathogen that forms a serious problem for immunocompromised patients and also the leading cause of mortality in cystic fibrosis. the overall importance of a functional type iii secretion system (t3ss) in p. aeruginosa virulence has been well established, but the underlying mechanisms are still unclear. using in vitro infected macrophages as w as a murine model of acute lung infection, we show that the caspase-1 mediated maturation and secretio ... | 2008 | 18081695 |
| structure and function of pa4872 from pseudomonas aeruginosa, a novel class of oxaloacetate decarboxylase from the pep mutase/isocitrate lyase superfamily. | pseudomonas aeruginosa pa4872 was identified by sequence analysis as a structurally and functionally novel member of the pep mutase/isocitrate lyase superfamily and therefore targeted for investigation. substrate screens ruled out overlap with known catalytic functions of superfamily members. the crystal structure of pa4872 in complex with oxalate (a stable analogue of the shared family alpha-oxyanion carboxylate intermediate/transition state) and mg2+ was determined at 1.9 a resolution. as with ... | 2008 | 18081320 |
| structure and function of pa4872 from pseudomonas aeruginosa, a novel class of oxaloacetate decarboxylase from the pep mutase/isocitrate lyase superfamily. | pseudomonas aeruginosa pa4872 was identified by sequence analysis as a structurally and functionally novel member of the pep mutase/isocitrate lyase superfamily and therefore targeted for investigation. substrate screens ruled out overlap with known catalytic functions of superfamily members. the crystal structure of pa4872 in complex with oxalate (a stable analogue of the shared family alpha-oxyanion carboxylate intermediate/transition state) and mg2+ was determined at 1.9 a resolution. as with ... | 2008 | 18081320 |
| gac two-component system in pseudomonas syringae pv. tabaci is required for virulence but not for hypersensitive reaction. | pseudomonas syringae pv. tabaci 6605 causes wildfire disease on host tobacco plants. to investigate the regulatory mechanism of the expression of virulence, gac two-component system-defective mutants, deltagaca and deltagacs, and a double mutant, deltagacadeltagacs, were generated. these mutants produced smaller amounts of n-acyl homoserine lactones required for quorum sensing, had lost swarming motility, and had reduced expression of virulence-related hrp genes and the algt gene required for ex ... | 2008 | 18080141 |
| rna recognition by 3'-to-5' exonucleases: the substrate perspective. | the 3'-to-5' exonucleolytic decay and processing of a variety of rnas is an essential feature of rna metabolism in all cells. the 3'-5' exonucleases, and in particular the exosome, are involved in a large number of pathways from 3' processing of rrna, snrna and snorna, to decay of mrnas and mrna surveillance. the potent enzymes performing these reactions are regulated to prevent processing of inappropriate substrates whilst mature rna molecules exhibit several attributes that enable them to evad ... | 2007 | 18078842 |
| rna recognition by 3'-to-5' exonucleases: the substrate perspective. | the 3'-to-5' exonucleolytic decay and processing of a variety of rnas is an essential feature of rna metabolism in all cells. the 3'-5' exonucleases, and in particular the exosome, are involved in a large number of pathways from 3' processing of rrna, snrna and snorna, to decay of mrnas and mrna surveillance. the potent enzymes performing these reactions are regulated to prevent processing of inappropriate substrates whilst mature rna molecules exhibit several attributes that enable them to evad ... | 2007 | 18078842 |
| modulation of bacterial type iii secretion system by a spermidine transporter dependent signaling pathway. | many gram-negative bacterial pathogens employ type iii secretion systems (t3ss) to inject effector proteins into host cells in infectious processes. | 2007 | 18074016 |
| complete dna sequence and analysis of the transferable multiple-drug resistance plasmids (r plasmids) from photobacterium damselae subsp. piscicida isolates collected in japan and the united states. | photobacterium damselae subsp. piscicida is a bacterial fish pathogen that causes a disease known as pasteurellosis. two transferable multiple-drug resistance (r) plasmids, pp99-018 (carrying resistance to kanamycin, chloramphenicol, tetracycline, and sulfonamide) and pp91278 (carrying resistance to tetracycline, trimethoprim, and sulfonamide), isolated from p. damselae subsp. piscicida strains from japan (p99-018) and the united states (p91278), respectively, were completely sequenced and analy ... | 2008 | 18070959 |
| complete dna sequence and analysis of the transferable multiple-drug resistance plasmids (r plasmids) from photobacterium damselae subsp. piscicida isolates collected in japan and the united states. | photobacterium damselae subsp. piscicida is a bacterial fish pathogen that causes a disease known as pasteurellosis. two transferable multiple-drug resistance (r) plasmids, pp99-018 (carrying resistance to kanamycin, chloramphenicol, tetracycline, and sulfonamide) and pp91278 (carrying resistance to tetracycline, trimethoprim, and sulfonamide), isolated from p. damselae subsp. piscicida strains from japan (p99-018) and the united states (p91278), respectively, were completely sequenced and analy ... | 2008 | 18070959 |
| atnfxl1, an arabidopsis homologue of the human transcription factor nf-x1, functions as a negative regulator of the trichothecene phytotoxin-induced defense response. | trichothecenes are a closely related family of phytotoxins that are produced by phytopathogenic fungi. in arabidopsis, expression of atnfxl1, a homologue of the putative human transcription repressor nf-x1, was significantly induced by application of type a trichothecenes, such as t-2 toxin. an atnfxl1 mutant growing on medium lacking trichothecenes showed no phenotype, whereas a hypersensitivity phenotype was observed in t-2 toxin-treated atnfxl1 mutant plants. microarray analysis indicated tha ... | 2008 | 18069941 |
| two distinct forms of m-locus protein kinase localize to the plasma membrane and interact directly with s-locus receptor kinase to transduce self-incompatibility signaling in brassica rapa. | many flowering plants possess systems of self-incompatibility (si) to prevent inbreeding. in brassica, si recognition is controlled by the multiallelic gene complex (s-haplotypes) at the s-locus, which encodes both the male determinant s-locus protein 11 (sp11/scr) and the female determinant s-receptor kinase (srk). upon self-pollination, the s-haplotype-specific interaction between the pollen-borne sp11 and the cognate stigmatic srk receptor induces si signaling in the stigmatic papilla cell an ... | 2007 | 18065692 |
| interaction between sgt1 and cytosolic/nuclear hsc70 chaperones regulates arabidopsis immune responses. | the conserved eukaryotic protein sgt1 (for suppressor of g2 allele of skp1) has characteristics of an hsp90 (for heat shock protein 90 kd) cochaperone and in plants regulates hormone responses and resistance gene-triggered immunity. we affinity-purified sgt1-interacting proteins from arabidopsis thaliana leaf extracts and identified by mass spectrometry cytosolic heat shock cognate 70 (hsc70) chaperones as the major stable sgt1 interactors. arabidopsis sgt1a and sgt1b proteins associate with hsc ... | 2007 | 18065690 |
| transgenic expression of a fungal endo-polygalacturonase increases plant resistance to pathogens and reduces auxin sensitivity. | polygalacturonases (pgs), enzymes that hydrolyze the homogalacturonan of the plant cell wall, are virulence factors of several phytopathogenic fungi and bacteria. on the other hand, pgs may activate defense responses by releasing oligogalacturonides (ogs) perceived by the plant cell as host-associated molecular patterns. tobacco (nicotiana tabacum) and arabidopsis (arabidopsis thaliana) plants expressing a fungal pg (pg plants) have a reduced content of homogalacturonan. here, we show that pg pl ... | 2008 | 18065558 |
| firefly luciferase complementation imaging assay for protein-protein interactions in plants. | the development of sensitive and versatile techniques to detect protein-protein interactions in vivo is important for understanding protein functions. the previously described techniques, fluorescence resonance energy transfer and bimolecular fluorescence complementation, which are used widely for protein-protein interaction studies in plants, require extensive instrumentation. to facilitate protein-protein interaction studies in plants, we adopted the luciferase complementation imaging assay. t ... | 2008 | 18065554 |
| moraxella catarrhalis synthesizes an autotransporter that is an acid phosphatase. | moraxella catarrhalis o35e was shown to synthesize a 105-kda protein that has similarity to both acid phosphatases and autotransporters. the n-terminal portion of the m. catarrhalis acid phosphatase a (mapa) was most similar (the blast probability score was 10(-10)) to bacterial class a nonspecific acid phosphatases. the central region of the mapa protein had similarity to passenger domains of other autotransporter proteins, whereas the c-terminal portion of mapa resembled the translocation doma ... | 2008 | 18065547 |
| moraxella catarrhalis synthesizes an autotransporter that is an acid phosphatase. | moraxella catarrhalis o35e was shown to synthesize a 105-kda protein that has similarity to both acid phosphatases and autotransporters. the n-terminal portion of the m. catarrhalis acid phosphatase a (mapa) was most similar (the blast probability score was 10(-10)) to bacterial class a nonspecific acid phosphatases. the central region of the mapa protein had similarity to passenger domains of other autotransporter proteins, whereas the c-terminal portion of mapa resembled the translocation doma ... | 2008 | 18065547 |
| interplay of different transporters in the mediation of divalent heavy metal resistance in pseudomonas putida kt2440. | according to in silico analysis, the genome of pseudomonas putida kt2440 encodes at least four zn/cd/pb efflux transporters-two p-type atpases (cada1 and cada2) and two czc chemiosmotic transporters (czccba1 and czccba2). in this study we showed that all these transporters are functional, but under laboratory conditions only two of them were involved in the mediation of heavy metal resistance in p. putida kt2440. cada2 conferred cd(2+) and pb(2+) resistance, whereas czccba1 was involved in expor ... | 2008 | 18065533 |
| interplay of different transporters in the mediation of divalent heavy metal resistance in pseudomonas putida kt2440. | according to in silico analysis, the genome of pseudomonas putida kt2440 encodes at least four zn/cd/pb efflux transporters-two p-type atpases (cada1 and cada2) and two czc chemiosmotic transporters (czccba1 and czccba2). in this study we showed that all these transporters are functional, but under laboratory conditions only two of them were involved in the mediation of heavy metal resistance in p. putida kt2440. cada2 conferred cd(2+) and pb(2+) resistance, whereas czccba1 was involved in expor ... | 2008 | 18065533 |
| expression of two old yellow enzyme homologues from gluconobacter oxydans and identification of their citral hydrogenation abilities. | two genes that encode proteins which share 30-35% sequence identity with yeast oye (old yellow enzyme, an nad(p)h fmn-oxidoreductase), the well-studied archetype of the oye protein family, have been identified in gluconobacter oxydans m5. the two genes are localized in the chromosome and plasmid, respectively. comparison of the deduced amino acid sequences of the enzymes with database entries revealed 75.1% similarity and 64.9% identity to that of the pseudomonas syringae pv. glycinea nad(p)h-de ... | 2008 | 18064575 |
| structure and dynamics of the antifungal molecules syringotoxin-b and syringopeptin-25a from molecular dynamics simulation. | the phytopathogen pseudomonas syringae pv. syringae produces toxic cyclic lipodepsipeptides (clps): nona-peptides and syringopeptins. all clps inhibit the growth of many fungal species, including human pathogens, although different fungi display different degrees of sensitivity. the best studied clps are syringomycin-e (sr-e), syringotoxin-b (st-b) and syringopeptin-25a (sp-25a). their biological activity is affected by membrane composition and their structural differences. we previously (matyus ... | 2008 | 18064450 |
| evolution of the chaperone/usher assembly pathway: fimbrial classification goes greek. | many proteobacteria use the chaperone/usher pathway to assemble proteinaceous filaments on the bacterial surface. these filaments can curl into fimbrial or nonfimbrial surface structures (e.g., a capsule or spore coat). this article reviews the phylogeny of operons belonging to the chaperone/usher assembly class to explore the utility of establishing a scheme for subdividing them into clades of phylogenetically related gene clusters. based on usher amino acid sequence comparisons, our analysis s ... | 2007 | 18063717 |
| 62-kb plasmids harboring rulab homologues confer uv-tolerance and epiphytic fitness to pseudomonas syringae pv. syringae mango isolates. | the presence of genetic determinants homologous to rulab genes for ultraviolet (uv) radiation resistance was determined in a collection of pseudomonas syringae pv. syringae strains isolated from mango. the potential role of these plasmids in uv tolerance and ecological fitness in the mango phyllosphere was also evaluated. nearly all of the 62-kb plasmids present in the p. syringae pv. syringae strains hybridized with a rulab probe, but these 62-kb plasmids showed differences in restriction patte ... | 2008 | 18058161 |
| chloroplast vector systems for biotechnology applications. | 2007 | 18056863 | |
| pseudomonas syringae type iii effector avrrpt2 alters arabidopsis thaliana auxin physiology. | the pseudomonas syringae type iii effector avrrpt2 promotes bacterial virulence on arabidopsis thaliana plants lacking a functional rps2 gene (rps2 mutant plants). to investigate the mechanisms underlying the virulence activity of avrrpt2, we examined the phenotypes of transgenic a. thaliana rps2 seedlings constitutively expressing avrrpt2. these seedlings exhibited phenotypes reminiscent of a. thaliana mutants with altered auxin physiology, including longer primary roots, increased number of la ... | 2007 | 18056646 |
| network inference, analysis, and modeling in systems biology. | 2007 | 18055607 | |
| identification of two catalases in azotobacter vinelandii: a katg homologue and a novel bacterial cytochrome c catalase, cccav. | azotobacter vinelandii produces two detectable catalases during growth on minimal medium. the heat-labile catalase expressed during exponential growth phase was identified as a katg homologue by liquid chromatography-tandem mass spectrometry (lc-ms/ms) using a mixed protein sample. the second catalase was heat resistant and had substantial residual activity after treatment at 90 degrees c. this enzyme was purified by anion-exchange and size exclusion chromatography and was found to exhibit stron ... | 2008 | 18055590 |
| identification of two catalases in azotobacter vinelandii: a katg homologue and a novel bacterial cytochrome c catalase, cccav. | azotobacter vinelandii produces two detectable catalases during growth on minimal medium. the heat-labile catalase expressed during exponential growth phase was identified as a katg homologue by liquid chromatography-tandem mass spectrometry (lc-ms/ms) using a mixed protein sample. the second catalase was heat resistant and had substantial residual activity after treatment at 90 degrees c. this enzyme was purified by anion-exchange and size exclusion chromatography and was found to exhibit stron ... | 2008 | 18055590 |
| core genome responses involved in acclimation to high temperature. | plants can acclimate rapidly to environmental conditions, including high temperatures. to identify molecular events important for acquired thermotolerance, we compared viability and transcript profiles of arabidopsis thaliana treated to severe heat stress (45 degrees c) without acclimation or following two different acclimation treatments. notably, a gradual increase to 45 degrees c (22 degrees c to 45 degrees c over 6 h) led to higher survival and to more and higher-fold transcript changes than ... | 2008 | 18055584 |
| intraspecific variation in a generalist herbivore accounts for differential induction and impact of host plant defences. | plants and herbivores are thought to be engaged in a coevolutionary arms race: rising frequencies of plants with anti-herbivore defences exert pressure on herbivores to resist or circumvent these defences and vice versa. owing to its frequency-dependent character, the arms race hypothesis predicts that herbivores exhibit genetic variation for traits that determine how they deal with the defences of a given host plant phenotype. here, we show the existence of distinct variation within a single he ... | 2007 | 18055390 |
| intraspecific variation in a generalist herbivore accounts for differential induction and impact of host plant defences. | plants and herbivores are thought to be engaged in a coevolutionary arms race: rising frequencies of plants with anti-herbivore defences exert pressure on herbivores to resist or circumvent these defences and vice versa. owing to its frequency-dependent character, the arms race hypothesis predicts that herbivores exhibit genetic variation for traits that determine how they deal with the defences of a given host plant phenotype. here, we show the existence of distinct variation within a single he ... | 2007 | 18055390 |
| a two-strain mixture of rhizobacteria elicits induction of systemic resistance against pseudomonas syringae and cucumber mosaic virus coupled to promotion of plant growth on arabidopsis thaliana. | we evaluated a commercial biopreparation of plant growth-promoting rhizobacteria (pgpr) strains bacillus subtilis gb03 and b. amyloliquefaciens in937a formulated with the carrier chitosan (bioyield) for its capacity to elicit growth promotion and induced systemic resistance against infection by cucumber mosaic virus (cmv) and pseudomonas syringae pv. tomato dc3000 in arabidopsis thaliana. the biopreparation promoted plant growth of arabidopsis hormonal mutants, which included auxin, gibberellic ... | 2007 | 18051759 |
| ectopic expression of apple mbr7 gene induced enhanced resistance to transgenic arabidopsis plant against a virulent pathogen. | a disease resistance related gene, mbr7, was identified in the wild apple species, malus baccata. the mbr7 gene has a single open reading frame (orf) of 3,288 nucleotides potentially encoding a 1,095-amino acid protein. its deduced amino acid sequence resembles the n protein of tobacco and the nl27 gene of potato and has several motifs characteristic of a tir-nbs-lrr r gene subclass. ectopic expression of mbr7 in arabidopsis enhanced the resistance against a virulent pathogen, pseudomonas syring ... | 2007 | 18051364 |
| proteomic analysis of the proteins regulated by hrpb from the plant pathogenic bacterium burkholderia glumae. | plant pathogenic bacteria transfer effector proteins into plant cells via the hypersensitive response and pathogenicity (hrp) type iii protein secretion system (t3ss) during infection. the genes encoding the hrp t3ss are expressed only under plant apoplast-mimicking conditions in an arac-type transcriptional activator hrpb-dependent manner. to identify the proteins controlled by hrpb in burkholderia glumae in vitro, we constitutively expressed hrpb and analyzed the proteins showing altered expre ... | 2008 | 18050277 |
| calcium efflux as a component of the hypersensitive response of nicotiana benthamiana to pseudomonas syringae. | using a model plant nicotiana benthamiana, we have demonstrated that initial calcium uptake in response to the hr (hypersensitive response)-causing pathogen pseudomonas syringae pv syringae 61 is followed by net calcium efflux initiated at about 12 h after the bacterial challenge and sustained for at least 48 h. our data suggest that calcium not only acts as an important second messenger in the activation of resistance responses but may also be a downstream mediator of later cell death accelerat ... | 2008 | 18048411 |
| bioinformatic evaluation of l-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of l-arginine catabolism in the cyanobacterium synechocystis sp. pcc 6803. | so far very limited knowledge exists on l-arginine catabolism in cyanobacteria, although six major l-arginine-degrading pathways have been described for prokaryotes. thus, we have performed a bioinformatic analysis of possible l-arginine-degrading pathways in cyanobacteria. further, we chose synechocystis sp. pcc 6803 for a more detailed bioinformatic analysis and for validation of the bioinformatic predictions on l-arginine catabolism with a transcript analysis. | 2007 | 18045455 |
| pathogen resistance of transgenic tobacco plants producing caffeine. | caffeine (1,3,7-trimethylxanthine) is a typical purine alkaloid, and produced by a variety of plants such as coffee and tea. its physiological function, however, is not completely understood, but chemical defense against pathogens and herbivores, and allelopathic effects against competing plant species have been proposed. previously, we constructed transgenic tobacco plants, which produced caffeine up to 5 microg per gram fresh weight of leaves, and showed them to repel caterpillars of tobacco c ... | 2008 | 18036626 |
| identification of prophages in bacterial genomes by dinucleotide relative abundance difference. | prophages are integrated viral forms in bacterial genomes that have been found to contribute to interstrain genetic variability. many virulence-associated genes are reported to be prophage encoded. present computational methods to detect prophages are either by identifying possible essential proteins such as integrases or by an extension of this technique, which involves identifying a region containing proteins similar to those occurring in prophages. these methods suffer due to the problem of l ... | 2007 | 18030328 |
| preliminary investigations on inducing salt tolerance in maize through inoculation with rhizobacteria containing acc deaminase activity. | twenty rhizobacterial strains containing 1-aminocyclopropane-1-carboxylate deaminase were isolated from the rhizosphere of salt-affected maize fields. they were screened for their growth-promoting activities under axenic conditions at 1, 4, 8, and 12 ds x m-1 salinity levels. based upon the data of the axenic study, the 6 most effective strains were selected to conduct pot trials in the wire house. besides one original salinity level (1.6 ds x m-1), 3 other salinity levels (4, 8, and 12 ds x m-1 ... | 2007 | 18026206 |
| effects of glycosylation on swimming ability and flagellar polymorphic transformation in pseudomonas syringae pv. tabaci 6605. | the role of flagellin glycosylation on motility was investigated in pseudomonas syringae pv. tabaci. the swimming activity of glycosylation-defective mutants was prominently decreased in a highly viscous medium. the mutants showed differences in polymorphic transitions and in the bundle formation of flagella, indicating that glycosylation stabilizes the filament structure and lubricates the rotation of the bundle. | 2008 | 18024523 |
| effects of glycosylation on swimming ability and flagellar polymorphic transformation in pseudomonas syringae pv. tabaci 6605. | the role of flagellin glycosylation on motility was investigated in pseudomonas syringae pv. tabaci. the swimming activity of glycosylation-defective mutants was prominently decreased in a highly viscous medium. the mutants showed differences in polymorphic transitions and in the bundle formation of flagella, indicating that glycosylation stabilizes the filament structure and lubricates the rotation of the bundle. | 2008 | 18024523 |