Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| performing comparative peptidomics analyses of salmonella from different growth conditions. | host-pathogen interactions are complex competitions during which both the host and the pathogen adapt rapidly to each other in order for one or the other to survive. salmonella enterica serovar typhimurium is a pathogen with a broad host range that causes a typhoid fever-like disease in mice and severe food poisoning in humans. the murine typhoid fever is a systemic infection in which s. typhimurium evades part of the immune system by replicating inside macrophages and other cells. the transitio ... | 2010 | 20013197 |
| microbial alkaloid staurosporine induces formation of nanometer-wide membrane tubular extensions (cytonemes, membrane tethers) in human neutrophils. | in the present work, we demonstrate that microbial alkaloid staurosporine (sts) and ro 31-8220, structurally related to sts protein kinase c inhibitor, caused development of membrane tubular extensions in human neutrophils upon adhesion to fibronectin-coated substrata. sts-induced tubular extensions interconnected neutrophils in a network and bound serum-opsonized bacteria salmonella enterica serovar typhimurium. the diameter of sts-induced extensions varied in the range 160-200 nm. the extensio ... | 2010 | 20009568 |
| enteric fever in mediterranean north africa. | typhoid fever is endemic in the mediterranean north african countries (morocco, algeria, tunisia, libya, and egypt) with an estimated incidence of 10-100 cases per 100,000 persons. outbreaks caused by salmonella enterica serovar typhi are common and mainly due to the consumption of untreated or sewage-contaminated water. salmonella enterica paratyphi b is more commonly involved in nosocomial cases of enteric fever in north africa than expected and leads to high mortality rates among infants with ... | 2009 | 20009276 |
| regulation of salmonella enterica pathogenicity island 1 by dna adenine methylation. | dna adenine methylase (dam(-)) mutants of salmonella enterica are attenuated in the mouse model and present multiple virulence-related defects. impaired interaction of salmonella dam(-) mutants with the intestinal epithelium has been tentatively correlated with reduced secretion of pathogenicity island 1 (spi-1) effectors. in this study, we show that s. enterica dam(-) mutants contain lowered levels of the spi-1 transcriptional regulators hila, hilc, hild, and invf. epistasis analysis indicates ... | 2010 | 20008574 |
| application of a short, disordered n-terminal flagellin segment, a fully functional flagellar type iii export signal, to expression of secreted proteins. | recently, we have demonstrated that the 26-47 segment of salmonella enterica serovar typhimurium flagellin is capable of mediating flagellar export. in order to reveal whether other parts of the n-terminal region have any significant influence on secretion, a series of plasmids were constructed containing the lac promoter followed by the 26-47, 2-65, or 2-192 portion of salmonella flagellin, to which various heterologous proteins of different size were fused (18 constructs overall). essentially, ... | 2010 | 20008166 |
| identification of conserved amino acid residues of the salmonella sigmas chaperone crl involved in crl-sigmas interactions. | proteins that bind sigma factors typically attenuate the function of the sigma factor by restricting its access to the rna polymerase (rnap) core enzyme. an exception to this general rule is the crl protein that binds the stationary-phase sigma factor sigma(s) (rpos) and enhances its affinity for the rnap core enzyme, thereby increasing expression of sigma(s)-dependent genes. analyses of sequenced bacterial genomes revealed that crl is less widespread and less conserved at the sequence level tha ... | 2010 | 20008066 |
| resistance development profiling of piperacillin in combination with the novel {beta}-lactamase inhibitor bli-489. | to evaluate development of resistance to the piperacillin/bli-489 combination. | 2010 | 20008048 |
| a live oral recombinant salmonella enterica serovar typhimurium vaccine expressing clostridium perfringens antigens confers protection against necrotic enteritis in broiler chickens. | necrotic enteritis (ne) in broiler chickens is caused by clostridium perfringens, and there is currently no effective vaccine for ne. we previously showed that in broiler chickens protection against ne can be achieved through intramuscular immunization with alpha toxin (at) and hypothetical protein (hp), and we subsequently identified b-cell epitopes in hp. in the present study, we identified b-cell epitopes in at recognized by chickens immune to ne. the gene fragments encoding immunodominant ep ... | 2010 | 20007363 |
| thioridazine protects the mouse from a virulent infection by salmonella enterica serovar typhimurium 74. | when administered to mice at doses of 100microg/mouse and 200microg/mouse, thioridazine (tdz) significantly protected animals from the lethality produced by a virulent strain of salmonella enterica serovar typhimurium and reduced the number of bacteria retrieved from the spleen, liver and heart blood. the protection conferred by tdz against a virulent salmonella infection is hypothesised to be due to a reduction in the 55kda virulence protein of the outer membrane of the organism, as this protei ... | 2010 | 20005679 |
| spray washing of tomatoes with chlorine dioxide to minimize salmonella on inoculated fruit surfaces and cross-contamination from revolving brushes. | chlorine dioxide (clo(2)) is an antimicrobial agent available for commercial produce washing. this study examined the efficacy of clo(2) at 5 parts per million (ppm) during spray washing of tomatoes (5.0 ml/s per fruit) for preventing salmonella enterica transfer from inoculated roller brushes to fruit and wash runoff. furthermore, the sanitizing effects of clo(2) during spray washing at low and high flow rates (5.0 and 9.3 ml/s per fruit, respectively) on tomato surfaces (nonstem scar areas) wi ... | 2009 | 20003724 |
| the transcriptional programme of salmonella enterica serovar typhimurium reveals a key role for tryptophan metabolism in biofilms. | biofilm formation enhances the capacity of pathogenic salmonella bacteria to survive stresses that are commonly encountered within food processing and during host infection. the persistence of salmonella within the food chain has become a major health concern, as biofilms can serve as a reservoir for the contamination of food products. while the molecular mechanisms required for the survival of bacteria on surfaces are not fully understood, transcriptional studies of other bacteria have demonstr ... | 2009 | 20003355 |
| salicylidene acylhydrazide-mediated inhibition of type iii secretion system-1 in salmonella enterica serovar typhimurium is associated with iron restriction and can be reversed by free iron. | salmonella enterica serovar typhimurium is an animal and zoonotic pathogen of worldwide importance. intestinal colonization, induction of enteritis and systemic translocation by this bacterium requires type iii protein secretion. strategies that target this process have the potential to control infection, pathology and transmission. we defined the global transcriptional response of s. typhimurium to inp0403, a member of a family of salicylidene acylhydrazides that inhibit type iii secretion (t3s ... | 2010 | 20002188 |
| molecular and pathogenic characterization of salmonella enterica serovar bovismorbificans strains of animal, environmental, food, and human origin in hungary. | in this study, we characterized 110 strains of salmonella enterica serovar bovismorbificans contaminating environment, animals, food of animal origin, and human, to assess their significance along the food chain in hungary. additionally, five strains from germany were tested for comparative purposes. characterization involved antibiotic susceptibility testing, class 1 integron detection by polymerase chain reaction, plasmid profiling, virulotyping (using virulence gene-specific polymerase chain ... | 2010 | 20001326 |
| validation of double digest selective label database for sequenced prokaryotic genomes. | summary: a database for simulation of double digest selective label (ddsl) typing technique has been created and validated against a sequenced strain (salmonella enterica serovar typhimurium strain lt2). in silico bands were in agreement with experimental, and the technique was able to discriminate among strains belonging to the same species. when compared with other strain discrimination techniques, ddsl showed a higher discriminatory power. the database contains precomputed data which may be s ... | 2010 | 19996164 |
| septic arthritis of the sternoclavicular joint caused by salmonella in a healthy person. | the sternoclavicular (sc) joint is an unusual site for septic arthritis in healthy people. it is mostly described as affecting intravenous drug abusers. we report a rare case of sternoclavicular osteo-arthritis caused by non-typhoid salmonella in an immunocompetent person. the patient presented with general weakness, dizziness, epigastric and anterior chest pain that had persisted for one week. peptic ulcer disease was diagnosed by gastro-intestinal endoscopy. computed tomography (ct) revealed f ... | 2009 | 19994814 |
| evolutionary diversification of an ancient gene family (rhs) through c-terminal displacement. | rhs genes are prominent features of bacterial genomes that have previously been implicated in genomic rearrangements in e. coli. by comparing rhs repertoires across the enterobacteriaceae, this study provides a robust explanation of rhs diversification and evolution, and a mechanistic model of how rhs diversity is gained and lost. | 2009 | 19968874 |
| high prevalence of salmonella in tegu lizards (tupinambis merianae), and susceptibility of the serotypes to antibiotics. | species of tegu (tupinambis) are the largest lizards in south america. large numbers of these lizards are hunted; there is a vigorous trade in their skins and the meat is consumed by rural and native peoples. the animals are also bred in captivity, an economic activity for rural populations which can help in the animals' conservation. faecal samples from 30 captive-born tegus were analysed for the presence of salmonella in two separate samplings. in the first analysis, samples from 26 animals (8 ... | 2010 | 19968856 |
| design, expression and characterization of recombinant hybrid peptide attacin-thanatin in escherichia coli. | antimicrobial peptides will be attractive and potential candidates as peptide drugs because of their efficient action against microbes and low toxicity to mammal cells. to improve their antibacterial activity, some modifications needs to be made. in this research, the hybrid peptide gene attacin-thanatin with 642 bp in length with preferred codons of e. coli was generated using the technology of gene splicing by overlap extension. the gene was inserted in-frame into e. coli expression plasmid pe ... | 2010 | 19967452 |
| size-dependent pcr inhibitory effect induced by gold nanoparticles. | in this work, the effect of gold nanoparticles (aunps) with diameters of around 5, 10 and 20 nm on pcr efficiency is evaluated respectively using a real-time pcr machine. gold nanoparticles show no obvious effect on pcr at low particle concentration. when the concentration is increased, pcr inhibition is observed. at the same particle concentration, gold nanoparticles of different sizes show different inhibitory effects on pcr. it is found that taq polymerase can interact with aunps. the interac ... | 2009 | 19964596 |
| phosphoribosylpyrophosphate synthetase (prsa) variants alter cellular pools of ribose 5-phosphate and influence thiamine synthesis in salmonella enterica. | phosphoribosylamine (pra) is the first intermediate in the common purine/thiamine biosynthetic pathway and is primarily synthesized by the product of the purf gene, glutamine phosphoribosylpyrophosphate (prpp) amidotransferase (e.c. 2.4.2.14). past genetic and biochemical studies have shown that multiple mechanisms for the synthesis of pra independent of purf are present in salmonella enterica. here, we describe mutant alleles of the essential prsa gene, which encodes prpp synthetase (e.c. 2.7.6 ... | 2010 | 19959576 |
| antimicrobial drug use and antimicrobial resistance in enteric bacteria among cattle from alberta feedlots. | the purpose of this study was to determine whether antimicrobial resistance (amr) in foodborne pathogens (escherichia coli o157, salmonella, and campylobacter) and non-type-specific e. coli obtained from fecal samples of feedlot cattle was associated with antimicrobial drug (amd) use. a secondary objective was to determine if amr in non-type-specific e. coli could be used as a predictor of amr in foodborne pathogens. fecal samples were collected from pen floors in 21 alberta feedlots during marc ... | 2010 | 19958100 |
| the salmonella spi2 effector ssei mediates long-term systemic infection by modulating host cell migration. | host-adapted strains of salmonella enterica cause systemic infections and have the ability to persist systemically for long periods of time despite the presence of a robust immune response. chronically infected hosts are asymptomatic and transmit disease to naïve hosts via fecal shedding of bacteria, thereby serving as a critical reservoir for disease. we show that the bacterial effector protein ssei (also called srfh), which is translocated into host cells by the salmonella pathogenicity island ... | 2009 | 19956712 |
| proteome of salmonella enterica serotype typhimurium grown in a low mg/ph medium. | to determine the impact of a low mg(2+)/ph defined growth medium (mgm) on the proteome of salmonella enterica serotype typhimurium, we cultured s. typhimurium cells in the medium under two different conditions termed mgm shock and mgm dilution and then comparatively analyzed the bacterial cells harvested from these conditions by a global proteomic approach. proteomic results showed that mgm shock and mgm dilution differentially affected the s. typhimurium proteome. mgm shock induced a group of p ... | 2009 | 19953200 |
| blacmy-2-positive inca/c plasmids from escherichia coli and salmonella enterica are a distinct component of a larger lineage of plasmids. | large multidrug resistance plasmids of the a/c incompatibility complex (inca/c) have been found in a diverse group of gram-negative commensal and pathogenic bacteria. we present three completed sequences from inca/c plasmids that originated from escherichia coli (cattle) and salmonella enterica serovar newport (human) and that carry the cephamycinase gene blacmy-2. these large plasmids (148 to 166 kbp) share extensive sequence identity and synteny. the most divergent plasmid, peh4h, has lost sev ... | 2010 | 19949054 |
| synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth. | we have previously described a murein hydrolase activity for the surface layer (s-layer) of lactobacillus acidophilus atcc 4356. here we show that, in combination with nisin, this s-layer acts synergistically to inhibit the growth of pathogenic gram-negative salmonella enterica and potential pathogenic gram-positive bacteria, staphylococcus aureus and bacillus cereus. in addition, bacteriolytic effects were observed for the gram-positive species tested. we postulate that the s-layer enhances the ... | 2010 | 19948852 |
| identification of the salmonella enterica damx gene product, an inner membrane protein involved in bile resistance. | the damx gene product of salmonella enterica serovar typhimurium is a protein located in the inner membrane. damx migrates as a 70-kda protein in sds-page even though the predicted protein size is 46 kda. synthesis of damx protein occurs in both exponential- and stationary-phase cultures. disruption of damx causes severe sensitivity to bile. lack of the outer membrane protein asma suppresses bile sensitivity in salmonella damx mutants. | 2010 | 19948803 |
| in salmonella enterica, 2-methylcitrate blocks gluconeogenesis. | strains of salmonella enterica serovar typhimurium lt2 lacking a functional 2-methylcitric acid cycle (2-mcc) display increased sensitivity to propionate. previous work from our group indicated that this sensitivity to propionate is in part due to the production of 2-methylcitrate (2-mc) by the krebs cycle enzyme citrate synthase (glta). here we report in vivo and in vitro data which show that a target of the 2-mc isomer produced by glta (2-mc(glta)) is fructose-1,6-bisphosphatase (fbpase), a ke ... | 2010 | 19948794 |
| some putative prebiotics increase the severity of salmonella enterica serovar typhimurium infection in mice. | prebiotics are non-digestible food ingredients believed to beneficially affect host health by selectively stimulating the growth of the beneficial bacteria residing in the gut. such beneficial bacteria have been reported to protect against pathogenic infections. however, contradicting results on prevention of salmonella infections with prebiotics have been published. the aim of the present study was to examine whether s. typhimurium sl1344 infection in mice could be prevented by administration o ... | 2009 | 19948011 |
| immobilization of bacteriophages on modified silica particles. | bacteriophages are selective anti-bacterial agents, which are receiving increasing acceptance by regulatory agencies for use both in the food industry and in clinical settings for biocontrol. while immobilized phage could be particularly useful to create antimicrobial surfaces, current immobilization strategies require chemical bioconjugation to surfaces or more difficult processes involving modification of their head proteins to express specific binding moieties, for example, biotin or cellulos ... | 2010 | 19945158 |
| quantitative analysis of the immune response upon salmonella typhimurium infection along the porcine intestinal gut. | salmonella enterica serovar typhimurium causes enteric disease and compromises food safety. in pigs, the molecular response of the intestine to s. typhimurium has been traditionally characterized by in vitro models that do not reflect the actual immunological competence of the intestinal mucosa. in this work, we performed an oral s. typhimurium infection study to obtain insight into the in vitro response in three different sections (jejunum, ileum and colon) of the porcine intestine. for this, s ... | 2010 | 19941811 |
| influence of rpos, camp-receptor protein, and ppgpp on expression of the opggh operon and osmoregulated periplasmic glucan content of salmonella enterica serovar typhimurium. | a transcriptional fusion (opgg1::mudj) to the opggh operon of salmonella enterica serovar typhimurium (s. typhimurium) lt2, isolated by resistance to mecillinam, was used to study the influence of global regulators rpos, ppgpp, and camp/camp-receptor protein (crp) on expression of the opggh operon and osmoregulated periplasmic glucan (opg) content. neither high growth medium osmolarity nor absence of ppgpp or crp had important effects on opgg1::mudj expression in exponential cultures. however, u ... | 2009 | 19940937 |
| study of an unusual paratyphoid epornitic in canaries (serinus canaria). | high mortalities in 17 canary flocks from different regions of tehran, iran, were reported. necropsy and histopathologic examination revealed necrotic hepatitis and overall congestive septicaemia in carcasses. salmonella enterica was isolated from 34 examined samples, two samples from each flock, including visceral organs of carcasses and droppings of live diseased birds. all isolates were typed as salmonella enterica serovar typhimurium by conventional serotyping. antibiotic resistance profilin ... | 2009 | 19937532 |
| a comparison of the efficacy of cleaning and disinfection methods in eliminating salmonella spp. from commercial egg laying houses. | effective terminal cleaning and disinfection (c&d) is regarded as a necessary step for the elimination of salmonella spp. from laying houses. a total of 60 commercial laying houses that had housed laying flocks infected with salmonella enterica serovar enteritidis or salmonella enterica serovar typhimurium that were representative of all production systems (cage, barn, free-range) were intensively sampled immediately after c&d as well as in the follow-on flock. the procedures investigated were: ... | 2009 | 19937529 |
| efficacy of several vaccination programmes in commercial layer and broiler breeder hens against experimental challenge with salmonella enterica serovar enteritidis. | two experiments were performed to evaluate the protective effect of various vaccination combinations given at 5 and 9 weeks of age against experimental challenge with salmonella enterica serovar enteritidis (se) phage type 4 at 12 weeks of age. in experiment 1, groups of commercial layers were vaccinated by one of the following programmes: group 1, two doses of a se bacterin (layermune se); group 2, one dose of a live salmonella enterica serovar gallinarum vaccine (cevac sg9r) followed by one do ... | 2009 | 19937524 |
| bovine muc1 inhibits binding of enteric bacteria to caco-2 cells. | inhibition of bacterial adhesion to intestinal epithelial receptors by the consumption of natural food components is an attractive strategy for the prevention of microbial related gastrointestinal illness. we hypothesised that muc1, a highly glycosylated mucin present in cows' milk, may be one such food component. purified bovine muc1 was tested for its ability to inhibit binding of common enteric bacterial pathogens to caco-2 cells grown in vitro. muc1 caused dose-dependent binding inhibition o ... | 2010 | 19936918 |
| prevalence of chlamydia trachomatis and neisseria gonorrhoeae in hiv-seropositive patients and gonococcal antimicrobial susceptibility: an update in thailand. | we surveyed the rate of chlamydial and gonococcal infections among human immunodeficiency virus (hiv)-seropositive patients in thailand as well as the current status of antimicrobial resistance of neisseria gonorrhoeae and determined the prevalence of penicillinase-producing n. gonorrhoeae (ppng) in thailand. a total of 1,158 endocervical swabs from 824 hiv-seropositive patients were collected to detect both organisms by gen-probe. the prevalences of chlamydial and gonococcal infection were 9.7 ... | 2009 | 19934542 |
| genome-wide transposon mutagenesis identifies a role for host neuroendocrine stress hormones in regulating the expression of virulence genes in salmonella. | bacterial sensing of environmental signals plays a key role in regulating virulence and mediating bacterium-host interactions. the sensing of the neuroendocrine stress hormones epinephrine (adrenaline) and norepinephrine (noradrenaline) plays an important role in modulating bacterial virulence. we used mudj transposon mutagenesis to globally screen for genes regulated by neuroendocrine stress hormones in salmonella enterica serovar typhimurium. we identified eight hormone-regulated genes, includ ... | 2010 | 19933366 |
| conversion of glycerol to poly(3-hydroxypropionate) in recombinant escherichia coli. | we have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3hp)]. for this, the genes for glycerol dehydratase (dhab1) of clostridium butyricum, propionaldehyde dehydrogenase (pdup) of salmonella enterica serovar typhimurium lt2, and polyhydroxyalkanoate (pha) synthase (phac1) of ralstonia eutropha were expressed in recombinant escherichia coli. poly(3hp) was accumulated up to 11.98% (wt/wt [cell dry weight]) in a two-step, fed-batch fermentation. the present ... | 2010 | 19933347 |
| cd8+ t cell adjuvant effects of salmonella flicd flagellin in live vaccine vectors or as purified protein. | salmonella flagellin, the flagellum structural subunit, has received particular interest as a vaccine adjuvant conferring enhanced immunogenity to soluble proteins or peptides, both for activation of antibody and cellular immune responses. in the present study, we evaluated the salmonella enterica flicd flagellin as a t cell vaccine adjuvant using as model the 9-mer (syvpsaeqi) synthetic h2(d)-restricted cd8(+) t cell-specific epitope (cs(280-288)) derived from the plasmodium yoelii circumsporoz ... | 2010 | 19932669 |
| salmonella enterica serovar typhimurium lipopolysaccharide deacylation enhances its intracellular growth within macrophages. | modification of lipid a is essential for bacterial adaptation to its host. salmonella typhimurium lpxr potentially detoxifies lipid a by 3'-o-deacylation; however, the involvement of deacylation in its adaptation remains unclear. lpxr-dependent 3'-o-deacylation was observed in the stationary phase. when macrophages were infected with stationary phase bacteria, the intracellular growth of the lpxr-null strain was lower than that of the wild-type strain. furthermore, the expression level of induci ... | 2010 | 19932105 |
| factors influencing survival of enterotoxigenic escherichia coli, salmonella enterica (serovar typhimurium) and vibrio parahaemolyticus in marine environments. | the presence and persistence of enterotoxigenic escherichia coli (etec) is poorly investigated in marine habitats. here we compared etec with the more studied fecal contaminant, salmonella enterica serotype typhimurium (s. enterica) and the marine bacteria vibrio parahaemolyticus. all three species of bacteria were culturable on agar plates during 8 weeks of incubation in seawater. however, the culturability of etec was positively affected by low temperature whereas v. parahaemolyticus was negat ... | 2010 | 19930458 |
| toll-like receptor 4 signalling through myd88 is essential to control salmonella enterica serovar typhimurium infection, but not for the initiation of bacterial clearance. | toll-like receptor-4 (tlr4) is important in protection against lethal salmonella enterica serovar typhimurium (s. typhimurium) infection. control of the early stages of sublethal s. typhimurium infection in mice depends on tlr4-dependent activation of macrophages and natural killer (nk) cells to drive an inflammatory response. tlr4 signals through the adapter proteins mal/myd88 and trif-related adaptor molecule (tram)/tir-domain-containing adaptor-inducing interferon-b (trif). in the mouse typho ... | 2009 | 19930040 |
| cellular cholesterol involvement in src, pkc, and p38/jnk transduction pathways by porins. | biological membranes are described as a mosaic of different domains where interactions between membrane components induce the formation of subdomains with different characteristics and functions. lipids play an important role in the formation of lipid-enriched microdomains where they dynamically associate to form platforms important for membrane protein sorting and construction of signaling complexes. cholesterol confined in lipid domains is a crucial component required by microorganisms, direct ... | 2009 | 19929574 |
| bacteriophage therapy of salmonella enterica: a fresh appraisal of bacteriophage therapy. | the most serious criticisms leveled at bacteriophage therapy are as follows: phages induce neutralizing antibodies, phages are active only when administered shortly after bacterial infection, and phage-resistant bacteria emerge rapidly in the course of therapy. | 2010 | 19929381 |
| binding of salmonella-specific antibody facilitates specific t cell responses via augmentation of bacterial uptake and induction of apoptosis in macrophages. | most antigens from intracellular bacteria or vaccines induce both humoral and cell-mediated immune responses, but interactions between these responses are not fully understood. this study aims to resolve how specific antibodies participate in the activation of specific t cells in protecting hosts against salmonella enterica serotype typhimurium (s. typhimurium) infection. | 2010 | 19929376 |
| salmonella enterica serovar typhimurium mutants completely lacking the f(0)f(1) atpase are novel live attenuated vaccine strains. | the f(0)f(1) atpase plays a central role in both the generation of atp and the utilisation of atp for cellular processes such as rotation of bacterial flagella. we have deleted the entire operon encoding the f(0)f(1) atpase, as well as genes encoding individual f(0) or f(1) subunits, in salmonella enteric serovar typhimurium. these mutants were attenuated for virulence, as assessed by bacterial counts in the livers and spleens of intravenously infected mice. the attenuated in vivo growth of the ... | 2010 | 19925904 |
| adjuvant effects for oral immunization provided by recombinant lactobacillus casei secreting biologically active murine interleukin-1{beta}. | vaccine delivery systems using lactic acid bacteria are under development, but their efficiency is insufficient. autologous cytokines, such as interleukin-1beta (il-1beta), are potential adjuvants for mucosal vaccines and can be provided by recombinant lactic acid bacteria. the aim of this study was the construction and evaluation of recombinant lactobacillus casei producing il-1beta as an adjuvant delivery agent. the recombinant strain was constructed using an expression/secretion vector plasmi ... | 2010 | 19923575 |
| genomic and phenotypic variation in epidemic-spanning salmonella enterica serovar enteritidis isolates. | salmonella enterica serovar enteritidis (s. enteritidis) has caused major epidemics of gastrointestinal infection in many different countries. in this study we investigate genome divergence and pathogenic potential in s. enteritidis isolated before, during and after an epidemic in uruguay. | 2009 | 19922635 |
| the phev phenylalanine trna gene klebsiella pneumoniae clinical isolates is an integration hotspot for possible niche-adaptation genomic islands. | horizontally acquired genomic islands may allow bacteria to conquer and colonize previously uncharted niches. four klebsiella pneumoniae trna gene insertion hotspots (arg6, asn34, met56, and phev) in 101 clinical isolates derived from blood, sputum, wound, bile or urine specimens were screened by long-range pcr for the presence or absence of integrated islands. the phev phenylalanine trna gene was the most frequently occupied site and harbored at least three entirely distinct types of islands: ( ... | 2010 | 19921332 |
| novel integron gene cassette arrays identified in a global collection of multi-drug resistant non-typhoidal salmonella enterica. | investigation of integron carriage in a global collection of multi-drug resistant salmonella enterica identified 3 unique class 1 integron gene cassette arrays not previously reported in this species. the present study used pcr and dna sequence analysis to characterize the structure of these gene cassette arrays. a approximately 4.0 kb integron containing the gene cassette array arr2/cmla5/bla (oxa10) /aada1 was found in isolates belonging to serovars isangi and typhimurium from south africa. a ... | 2010 | 19921331 |
| fitness costs and stability of a high-level ciprofloxacin resistance phenotype in salmonella enterica serotype enteritidis: reduced infectivity associated with decreased expression of salmonella pathogenicity island 1 genes. | the fitness costs associated with high-level fluoroquinolone resistance were examined for phenotypically and genotypically characterized ciprofloxacin-resistant salmonella enterica serotype enteritidis mutants (104-cip and 5408-cip; mic, >32 microg/ml). the stability of the fluoroquinolone resistance phenotype in both mutants was investigated to assess whether clones with better fitness could emerge in the absence of antibiotic selective pressure. mutants 104-cip and 5408-cip displayed altered m ... | 2010 | 19917752 |
| development of a dna microarray to detect antimicrobial resistance genes identified in the national center for biotechnology information database. | to understand the mechanisms and epidemiology of antimicrobial resistance (ar), the genetic elements responsible must be identified. due to the myriad of possible genes, a high-density genotyping technique is needed for initial screening. to achieve this, ar genes in the national center for biotechnology information genbank database were identified by their annotations and compiled into a nonredundant list of 775 genes. a dna microarray was constructed of 70mer oligonucelotide probes designed to ... | 2010 | 19916789 |
| improving the aoac use-dilution method by establishing a minimum log density value for test microbes on inoculated carriers. | the aoac use-dilution methods, 955.14 (salmonella enterica), 955.15 (staphylococcus aureus), and 964.02 (pseudomonas aeruginosa), are used to measure the efficacy of disinfectants on hard inanimate surfaces. the methods do not provide procedures to assess log density of the test microbe on inoculated penicylinders (carrier counts). without a method to measure and monitor carrier counts, the associated efficacy data may not be reliable and repeatable. this report provides a standardized procedure ... | 2009 | 19916390 |
| epidemiological characteristics of salmonella enterica serovar typhimurium from healthy pigs in japan. | we characterized 53 salmonella enterica ser. typhimurium strains recovered from healthy pigs during 1998-1999 (n=12) and 2004-2005 (n=41) as to their carriage of dt104 spacer region, class 1 and 2 integrons, virulence genes (spvc, rck, and pefa), and xbai- and blni-pulsed-field gel electrophoresis (pfge) profiles. no dt104 strain was detected in 1998-1999, whereas 65.9% (27/41) of the strains in 2004-2005 were dt104 showing resistance to ampicillin, chloramphenicol, streptomycin, sulfonamides, t ... | 2010 | 19915327 |
| effects of x-ray radiation on escherichia coli o157:h7, listeria monocytogenes, salmonella enterica and shigella flexneri inoculated on shredded iceberg lettuce. | the main goal of this investigation was to study the efficacy of x-ray doses (0.1, 0.2, 0.3, 0.5, 0.75, 1.0, 1.5 and 2.0 kgy) on inoculated escherichia coli o157: h7, listeria monocytogenes, salmonella enterica and shigella flexneri on shredded iceberg lettuce. the second goal was to study the effect of x-ray on the inherent microflora counts and visual color of shredded iceberg lettuce during storage at 4 degrees c for 30 days. treatment with 1.0 kgy x-ray significantly reduced the population o ... | 2010 | 19913700 |
| inactivation of escherichia coli o157:h7, listeria monocytogenes, salmonella enterica and shigella flexneri on spinach leaves by x-ray. | several recent foodborne disease outbreaks associated with leafy green vegetables, including spinach, have been reported. x-ray is a non-thermal technology that has shown promise for reducing pathogenic and spoilage bacteria on spinach leaves. inactivation of inoculated escherichia coli o157:h7, listeria monocytogenes, salmonella enterica and shigella flexneri on spinach leaves using x-ray at different doses (0.1, 0.2, 0.3, 0.5, 0.75, 1.0, 1.5 and 2.0 kgy) was studied. the effect of x-ray on col ... | 2010 | 19913687 |
| genome wide evolutionary analyses reveal serotype specific patterns of positive selection in selected salmonella serotypes. | the bacterium salmonella enterica includes a diversity of serotypes that cause disease in humans and different animal species. some salmonella serotypes show a broad host range, some are host restricted and exclusively associated with one particular host, and some are associated with one particular host species, but able to cause disease in other host species and are thus considered "host adapted". five salmonella genome sequences, representing a broad host range serotype (typhimurium), two host ... | 2009 | 19912661 |
| cluster of cases of salmonella enterica serotype rissen infection in a general hospital, italy, 2007. | in 2007, three strains of salmonella enterica serotype rissen (s. rissen) were isolated in the laboratory of diagnostic microbiology of the general hospital of prato, tuscany, italy, over a 1 month and half interval of time. the first isolate was recovered on january 26 from an outpatient with enteritis. then, two strains were isolated on february 16 and march 11 respectively, from central venous catheters of patients who were being hospitalized in two departments of the hospital. an epidemiolog ... | 2010 | 19912602 |
| real-time reverse-transcriptase--polymerase chain reaction for salmonella enterica detection from jalapeño and serrano peppers. | outbreaks of salmonella linked to fresh produce emphasize the need for rapid detection methods to curb the spread of foodborne pathogens. reverse-transcriptase-polymerase chain reaction (rt-pcr) detects the presence of mrna (shorter half-life than dna), with greater potential of detecting viable pathogens. real-time rt-pcr eliminates the need for gel electrophoresis and significantly enhances the speed of detection (<1 day) compared with traditional methods (>5 days). the objectives of this rese ... | 2010 | 19911882 |
| identification of in vivo induced protein antigens of salmonella enterica serovar typhi during human infection. | during infectious disease episodes, pathogens express distinct subsets of virulence factors which allow them to adapt to different environments. hence, genes that are expressed or upregulated in vivo are implicated in pathogenesis. we used in vivo induced antigen technology (iviat) to identify antigens which are expressed during infection with salmonella enterica serovar typhi. we identified 7 in vivo induced (ivi) antigens, which included bcfd (a fimbrial structural subunit), grxc (a glutaredox ... | 2009 | 19911130 |
| transcriptome analysis of genes controlled by luxs/autoinducer-2 in salmonella enterica serovar typhimurium. | the enteric pathogen salmonella enterica serovar typhimurium uses autoinducer-2 (ai-2) as a signaling molecule. ai-2 requires the luxs gene for its synthesis. the regulation of global gene expression in salmonella typhimurium by luxs/ai-2 is currently not known; therefore, the focus of this study was to elucidate the global gene expression patterns in salmonella typhimurium as regulated by luxs/ai-2. the genes controlled by luxs/ai-2 were identified using microarrays with rna samples from wild-t ... | 2010 | 19909098 |
| consumption of fresh fruit juice: how a healthy food practice caused a national outbreak of salmonella panama gastroenteritis. | in spring 2008, 15 salmonella panama laboratory-confirmed cases were reported within 2 weeks, twice the average annual number of reported cases of this infrequent serotype in the netherlands. to identify the source responsible for this national outbreak, we carried out an epidemiological, microbiological, and trace-back investigation. in total, 33 cases were reported, and a matched case-control study (23 cases/24 controls) identified consumption of fresh (unpasteurized) fruit juice purchased fro ... | 2010 | 19909088 |
| uptake through glycoprotein 2 of fimh(+) bacteria by m cells initiates mucosal immune response. | the mucosal immune system forms the largest part of the entire immune system, containing about three-quarters of all lymphocytes and producing grams of secretory iga daily to protect the mucosal surface from pathogens. to evoke the mucosal immune response, antigens on the mucosal surface must be transported across the epithelial barrier into organized lymphoid structures such as peyer's patches. this function, called antigen transcytosis, is mediated by specialized epithelial m cells. the molecu ... | 2009 | 19907495 |
| sensitive and rapid molecular detection assays for salmonella enterica serovars typhimurium and heidelberg. | salmonella enterica is a significant cause of gastroenteritis worldwide, with serovars typhimurium and heidelberg being particularly prevalent, which have broad host ranges infecting poultry, dairy animals, and humans. traditional methods used for the detection of salmonella from contaminated food products are time-consuming and labor-intensive. the aim of this study was to develop a sensitive and rapid pcr-based detection method with optimized specificity for high-throughput screening of food a ... | 2009 | 19903399 |
| transfer of plasmid-mediated ctx-m-9 from salmonella enterica serotype virchow to enterobacteriaceae in human flora-associated rats treated with cefixime. | food animals are a potential source of ctx-m resistance genes for humans. we evaluated the transfer of the bla(ctx-m-9) gene from an animal strain of salmonella enterica serotype virchow to enterobacteriaceae of the human intestinal flora by using human flora-associated (hfa) rats with and without cefixime treatment. in the absence of antibiotic, no transconjugant enterobacteria were found in the feces of hfa rats. however, the transfer rate was high if escherichia coli j5 recipient strains were ... | 2010 | 19901088 |
| the salmonella enterica serotype typhi vi capsular antigen is expressed after the bacterium enters the ileal mucosa. | salmonella enterica serotype typhi, the etiological agent of typhoid fever, produces the vi capsular antigen, a virulence factor absent in salmonella enterica serotype typhimurium. previous studies suggest that the capsule-encoding viab locus reduces inflammatory responses in intestinal tissue; however, there are currently no data regarding the in vivo expression of this locus. here we implemented direct and indirect methods to localize and detect vi antigen expression within polarized intestina ... | 2010 | 19901065 |
| epidemic multiple drug resistant salmonella typhimurium causing invasive disease in sub-saharan africa have a distinct genotype. | whereas most nontyphoidal salmonella (nts) are associated with gastroenteritis, there has been a dramatic increase in reports of nts-associated invasive disease in sub-saharan africa. salmonella enterica serovar typhimurium isolates are responsible for a significant proportion of the reported invasive nts in this region. multilocus sequence analysis of invasive s. typhimurium from malawi and kenya identified a dominant type, designated st313, which currently is rarely reported outside of africa. ... | 2009 | 19901036 |
| cells in shearable and nonshearable regions of salmonella enterica serovar enteritidis biofilms are morphologically and physiologically distinct. | cellular morphology, exopolymer chemistry, and protein expression of shearable and nonshearable fractions of salmonella enterica serovar enteritidis biofilms were examined. biofilms were grown at a laminar flow velocity of 0.07 cm.s-1 for ~120 h, resulting in biofilms with a thickness (mean +/- sd) of 43 +/- 24 microm. an empirically determined shear-inducing flow (1.33 cm.s-1) was then applied for 5 min, effectively reducing biofilm thickness by ~70% and leaving 13 +/- 6 microm of nonshearable ... | 2009 | 19898535 |
| promoter and riboswitch control of the mg2+ transporter mgta from salmonella enterica. | the mgta protein from salmonella enterica serovar typhimurium mediates mg(2+) uptake from the periplasm into the cytoplasm. here we report that the phop/phoq two-component regulatory system, which responds to periplasmic mg(2+), governs mgta transcription initiation at all investigated mg(2+) concentrations and that the mg(2+)-sensing 5' leader region of the mgta gene controls transcription elongation into the mgta coding region when salmonella is grown in media with <50 mum mg(2+). overexpressi ... | 2010 | 19897653 |
| bistable expression of csgd in biofilm development of salmonella enterica serovar typhimurium. | bacterial persistence in the environment and in the infected host is often aided by the formation of exopolymer-enclosed communities known as biofilms. heterogeneous gene expression takes place in microcompartments formed within the complex biofilm structure. this study describes cell differentiation within an isogenic bacterial cell population based on the example of biofilm formation by salmonella enterica serovar typhimurium. we analyzed the expression of the major biofilm regulator csgd at t ... | 2010 | 19897646 |
| short-term signatures of evolutionary change in the salmonella enterica serovar typhimurium 14028 genome. | salmonella enterica serovar typhimurium is a gram-negative pathogen that causes gastroenteritis in humans and a typhoid-like disease in mice and is often used as a model for the disease promoted by the human-adapted s. enterica serovar typhi. despite its health importance, the only s. typhimurium strain for which the complete genomic sequence has been determined is the avirulent lt2 strain, which is extensively used in genetic and physiologic studies. here, we report the complete genomic sequenc ... | 2010 | 19897643 |
| salmonella infection of a total knee replacement. | salmonella enterica serotype choleraesuis is a rare infective pathogen in total knee arthroplasty and accounts for less than 1% of all cases. in the united kingdom, the most prevalent organisms are staphylococci, which account for over 50% of cases. we present an interesting case report of an infected revision total knee replacement following foreign travel to a region with an increased prevalence of salmonella. the patient presented typically with an effused knee and raised inflammatory markers ... | 2010 | 19897369 |
| tlr2-, tlr4- and myd88-independent acquired humoral and cellular immunity against salmonella enterica serovar typhimurium. | toll-like receptors (tlr) are central to pathogen recognition by the innate immune system. however, their role in the generation of acquired immunity is less clear. using experimental salmonella enterica serovar typhimurium (st) infection of mice, we determined the role of tlr2, tlr4 and of the tlr adaptor protein myeloid differentiation factor 88 (myd88) in the generation of specific t- and b-cell responses against this pathogen. when infected with an attenuated st strain, mice deficient in tlr ... | 2010 | 19895846 |
| antibacterial effects of allspice, garlic, and oregano essential oils in tomato films determined by overlay and vapor-phase methods. | physical properties as well as antimicrobial activities against escherichia coli o157:h7, salmonella enterica, and listeria monocytogenes of allspice, garlic, and oregano essential oils (eos) in tomato puree film-forming solutions (tpffs) formulated into edible films at 0.5% to 3% (w/w) concentrations were investigated in this study. antimicrobial activities were determined by 2 independent methods: overlay of the film on top of the bacteria and vapor-phase diffusion of the antimicrobial from th ... | 2009 | 19895486 |
| effects of allspice, cinnamon, and clove bud essential oils in edible apple films on physical properties and antimicrobial activities. | essential oils (eos) derived from plants are rich sources of volatile terpenoids and phenolic compounds. such compounds have the potential to inactivate pathogenic bacteria on contact and in the vapor phase. edible films made from fruits or vegetables containing eos can be used commercially to protect food against contamination by pathogenic bacteria. eos from cinnamon, allspice, and clove bud plants are compatible with the sensory characteristics of apple-based edible films. these films could e ... | 2009 | 19895483 |
| antimicrobial substances produced by coliform strains active against foodborne pathogens. | in the present study, 31 coliform strains were isolated from salad, cheese, and meat products sold in commercial establishments in rio de janeiro city, and were tested for antibiotic resistance and antimicrobial substance production. thirteen strains (41.9%) were resistant to at least one antibiotic tested, among which one presented resistance to nine different antibiotics. two strains (6.4%) exhibited inhibitory activity against the indicator strains, escherichia coli lmifrj and salmonella ente ... | 2010 | 19895262 |
| case report of pediatric gastroenteritis due to ctx-m-15 extended-spectrum beta-lactamase-producing salmonella enterica serotype enteritidis. | a clinical isolate of salmonella enterica serotype enteritidis in korea was found to produce the extended-spectrum beta-lactamase ctx-m-15. the isolate was recovered in 2008 from the stool of a 3-yr-old boy with gastroenteritis. this isolate was found to be resistant to multiple drugs, including ampicillin, piperacillin, cefotaxime, ceftazidime, cefepime, and aztreonam. the resistance to cefotaxime was transferred by conjugation to recipient escherichia coli j53. the patient was eventually succe ... | 2009 | 19893356 |
| listeria monocytogenes does not survive ingestion by acanthamoeba polyphaga. | listeria monocytogenes is a ubiquitous bacterium capable of infecting humans, particularly pregnant women and immunocompromised individuals. although the intracellular invasion and pathogenesis of listeriosis in mammalian tissues has been well studied, little is known about the ecology of l. monocytogenes , and in particular the environmental reservoir for this bacterium has not been identified. this study used short-term co-culture at 15, 22 and 37 degrees c to examine the interaction of l. mon ... | 2010 | 19892759 |
| physical and chemical characterization and immunologic properties of salmonella enterica serovar typhi capsular polysaccharide-diphtheria toxoid conjugates. | typhoid fever remains a serious public health problem in developing countries, especially among young children. recent studies showed more than 50% of typhoid cases are in children under 5 years old. licensed vaccines, such as salmonella enterica serovar typhi capsular vi, did not confer protection against typhoid fever for this age group. vi conjugate, prepared by binding vi to pseudomonas aeruginosa recombinant exoprotein a (repa), induces protective levels of antibody at as young as 2 years o ... | 2010 | 19889941 |
| detection of mutations in the gyra gene in fluoroquinolone resistance salmonella enterica serotypes typhi and paratyphi a isolated from the infectious diseases hospital, kuwait. | enteric fever due to salmonella enterica is a major health problem, and fluoroquinolones such as ciprofloxacin are mostly the antibiotic of choice for treatment. resistance to ciprofloxacin has been noticed to increase due to the emergence of new mutations in the bacterial dna. | 2010 | 19889623 |
| inhibition of fungi and gram-negative bacteria by bacteriocin bactn635 produced by lactobacillus plantarum sp. tn635. | the aim of this study was to evaluate 54 lactic acid bacteria (lab) strains isolated from meat, fermented vegetables and dairy products for their capacity to produce antimicrobial activities against several bacteria and fungi. the strain designed tn635 has been selected for advanced studies. the supernatant culture of this strain inhibits the growth of all tested pathogenic including the four gram-negative bacteria (salmonella enterica atcc43972, pseudomonas aeruginosa atcc 49189, hafnia sp. and ... | 2010 | 19888697 |
| antimicrobial resistance genes in salmonella enterica isolates from poultry and swine in thailand. | a total of 184 resistant salmonella enterica isolated from poultry and swine were investigated for the distribution of and associations between various antimicrobial resistance genes. all the isolates were screened for the presence of class 2 and 3 integrase genes and 18 resistance genes corresponding to their resistance phenotypes. none of the isolates carried class 2 and 3 integrons. the investigated resistance genes were responsible for resistance in 78% of the isolates. all the strains harbo ... | 2009 | 19887742 |
| multi-immunogenic outer membrane vesicles derived from an msbb-deficient salmonella enterica serovar typhimurium mutant. | to develop low endotoxic and multi-immunogenic outer membrane vesicles (omvs), a deletion mutant of the msbb gene in salmonella enterica serovar typhimurium (s. typhimurium) was used as a source of low endotoxic omv, and an expression vector of the canine parvovirus (cpv) vp2 epitope fused to the bacterial ompa protein was constructed and transformed into the salmonella deltaamsbb mutant. in a lethality test, balb/c mice injected intraperitoneally with the salmonella deltaamsbb mutant survived f ... | 2009 | 19884791 |
| first report of extended-spectrum-beta-lactamase-producing salmonella enterica serovar kentucky isolated from poultry in ireland. | therapy of invasive human salmonellosis is complicated by increasing antimicrobial resistance. food animals are the principal source of infection with nontyphoid salmonella. we report the emergence of broad-spectrum-cephalosporin resistance in salmonella enterica serovar kentucky in poultry in ireland. | 2010 | 19884382 |
| unusual class 1 integron configuration found in salmonella genomic island 2 from salmonella enterica serovar emek. | salmonella genomic island 2 (sgi2) is an independently derived genomic island related to sgi1 with the integron in a different position. the integron in sgi2 was found to include an additional 2.1 kb derived from the tni module of tn5058, tn502, or tn512 that was not detected previously. independent evolution of the backbone was confirmed with 21 single base differences found in over 11.5 kb, representing 40% of the 27.4-kb sgi2 backbone. | 2010 | 19884375 |
| a new generation of stable, nonantibiotic, low-copy-number plasmids improves immune responses to foreign antigens in salmonella enterica serovar typhi live vectors. | we hypothesized that adequately engineered attenuated salmonella enterica serovar typhi strains can serve as multivalent mucosal live vector vaccines to immunize against unrelated human pathogens. toward this ultimate goal, we have developed a novel genetic stabilization system for antigen-expressing plasmids, engineered to encode the single-stranded binding protein (ssb), an essential protein involved in dna metabolism which was deleted from the live vector chromosome. we utilized full-length p ... | 2010 | 19884333 |
| norepinephrine augments salmonella enterica-induced enteritis in a manner associated with increased net replication but independent of the putative adrenergic sensor kinases qsec and qsee. | stress has long been correlated with susceptibility to microbial infection. one explanation for this phenomenon is the ability of pathogens to sense and respond to host stress-related catecholamines, such as norepinephrine (ne). in gram-negative enteric pathogens, it has been proposed that ne may facilitate growth by mediating iron supply, or it may alter gene expression by activating adrenergic sensor kinases. the aim of this work was to investigate the relative importance of these processes in ... | 2010 | 19884332 |
| salmonella enterica serovar typhimurium trxa mutants are protective against virulent challenge and induce less inflammation than the live-attenuated vaccine strain sl3261. | in salmonella enterica serovar typhimurium, trxa encodes thioredoxin 1, a small, soluble protein with disulfide reductase activity, which catalyzes thiol disulfide redox reactions in a variety of substrate proteins. thioredoxins are involved as antioxidants in defense against oxidative stresses, such as exposure to hydrogen peroxide and hydroxyl radicals. we have made a defined, complete deletion of trxa in the mouse-virulent s. typhimurium strain sl1344 (sl1344 trxa), replacing the gene with a ... | 2010 | 19884329 |
| multilocus variable-number tandem-repeat analysis and plasmid profiling to study the occurrence of blacmy-2 within a pulsed-field gel electrophoresis-defined clade of salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium circulating in food animal populations and carrying resistance to antimicrobial agents represents a human health risk. recently, a new clade of s. typhimurium, wa-typ035/187, was reported in cattle and humans in the pacific northwest, united states of america. the objective of this study was to describe a possible mechanism of acquisition of expanded-spectrum cephalosporin resistance in this clade. ceftazidime resistance increased steadily among wa-typ035/ ... | 2010 | 19880639 |
| sdia bridges chemical signaling between salmonella enterica serovar typhimurium and yersinia enterocolitica in mice. | 2010 | 19880607 | |
| functional analysis of the nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (cobt) enzyme, involved in the late steps of coenzyme b12 biosynthesis in salmonella enterica. | in salmonella enterica, the cobt enzyme activates the lower ligand base during the assembly of the nucleotide loop of adenosylcobalamin (adocbl) and other cobamides. previously, mutational analysis identified a class of alleles (class m) that failed to restore adocbl biosynthesis during intragenic complementation studies. to learn why class m cobt mutations were deleterious, we determined the nature of three class m cobt alleles and performed in vivo and in vitro functional analyses guided by av ... | 2010 | 19880598 |
| development of a live oral attaching and effacing escherichia coli vaccine candidate using vibrio cholerae cvd 103-hgr as antigen vector. | attaching and effacing escherichia coli (aeec) share the ability to induce pedestal formation and intimate adherence of the bacteria to the intestinal epithelial cell and effacement of microvilli of epithelial tissue. the locus of enterocyte effacement (lee) pathogenicity island encodes the ability to induce attaching and effacing (a/e) lesions and contains the gene eae, which encodes intimin, an outer membrane protein that is an adhesin for a/e lesion formation. here we show the utility of usin ... | 2010 | 19878715 |
| molecular basis and evolution of multiple drug resistance in the foodborne pathogen salmonella enterica serovar ohio. | the molecular basis and evolution of multidrug resistance were established for 54 isolates of salmonella enterica serovar ohio, recorded between 1991 and 2005 in asturias, a northern region of spain. all isolates were closely related, as shown by cluster analysis of xbai-blni combined profiles. of these, 33.3% were resistant to one or more unrelated agent(s). sulphonamides, streptomycin, tetracycline, and trimethoprim, encoded by sul1, aada1, tet(a) or tet(b), and dfra1, respectively, were the m ... | 2010 | 19877766 |
| the use of chromogenic bacteria as coloured substitutes for pathogens: a simple strategy during design and development of a new method for sample pretreatment. | in the present study, chromogenic (red) bacteria were used to simulate actual target bacteria during set-up and optimization of an isolation process of bacteria, designed for food samples. isolation of bacteria from food in the context of molecular biological detection of food pathogens is a multistep process. development of such a separation method requires continuous monitoring of the location of the presumable targets in the sample tubes. therefore, red-coloured pigmented bacteria were used a ... | 2010 | 19874486 |
| bacilli of the hog-cholera group (bacillus cholerae suis) in man. | 1. the organisms isolated by hirschfeld from febrile cases resembling paratyphoid fever and named paratyphoid c can be placed in the hog-cholera bacillus group by their agglutination absorption properties though they are not typical culturally. 2. when fed to a pig a febrile disease resulted from which the animal recovered. after injection of hog-cholera virus the organisms fed were found generally, distributed and some of them had lost cultural characters so that they are brought into the class ... | 1920 | 19868429 |
| a group of paratyphoid bacilli from animals closely resembling those found in man. | 1. in addition to the paratyphoid bacilli already named there exists a group which occurs in a variety of animals and which culturally is the same as bacillus schottmülleri. as a rule this group can be separated from the latter by the type of clumps formed when bouillon cultures are used as antigens, while other antigens and complement fixation tests have failed to differentiate it. agglutination absorption tests sharply separate the animal from the human paratyphoids. 2. no differences have bee ... | 1920 | 19868428 |
| genotyping, plasmid analysis, and antimicrobial susceptibility of salmonella enterica serotype enteritidis isolates from humans and chickens in central taiwan. | salmonella enterica serotype enteritidis (se) is the most frequent etiological agent of human salmonellosis. the molecular epidemiology and antimicrobial susceptibility of human and chicken isolates of se were examined. | 2009 | 19864196 |
| psgi15, a small cole-like qnrb19 plasmid of a salmonella enterica serovar typhimurium strain carrying salmonella genomic island 1 (sgi1). | 2010 | 19861336 | |
| ceftazidime-resistant salmonella enterica, morocco. | 2009 | 19861080 | |
| division of the salmonella-containing vacuole and depletion of acidic lysosomes in salmonella-infected host cells are novel strategies of salmonella enterica to avoid lysosomes. | salmonella has evolved several strategies to counteract intracellular microbicidal agents like reactive oxygen and nitrogen species. however, it is not yet clear how salmonella escapes lysosomal degradation. some studies have demonstrated that salmonella can inhibit phagolysosomal fusion, whereas other reports have shown that the salmonella-containing vacuole (scv) fuses/interacts with lysosomes. here, we have addressed this issue from a different perspective by investigating if the infected hos ... | 2010 | 19858305 |