Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| natural cell-mediated cytotoxicity against various target cells in patients with epidermodysplasia verruciformis. | natural cell-mediated cytotoxicity of peripheral blood mononuclear cells was studied in eight patients with epidermodysplasia verruciformis induced by human papillomaviruses specific for epidermodysplasia verruciformis and in five patients with epidermodysplasia verruciformis-induced exclusively by human papillomavirus type 3. nine patients with various cutaneous warts and 25 age- and sex-matched healthy persons were control subjects. natural cell-mediated cytotoxicity against both k-562 erythro ... | 1990 | 2155952 |
| thermus aquaticus dna polymerase-catalysed chain reaction for the detection of human papillomaviruses. | to delineate the conditions for the polymerase chain reaction (pcr) using primers specific for human papillomavirus (hpv) types 6b, 16 and 18, a number of important technical features were analysed. buffer, concentrations of magnesium, taq polymerase, primers and dna templates, annealing temperature, and extension time were studied by a combination of gel electrophoresis, southern and slot-blot hybridization. amplification of e6 gene fragments of hpv-16 and hpv-18 generated bands of 110 bp and 1 ... | 1990 | 2155246 |
| the dna-binding domain of hpv-16 e2 protein interaction with the viral enhancer: protein-induced dna bending and role of the nonconserved core sequence in binding site affinity. | we expressed the carboxy-terminal portion of the e2 open reading frame (orf)-encoded protein of human papillomavirus type 16 (hpv-16) and purified it to near homogeneity. using dnase i footprinting techniques, we show that like the homologous protein from bovine papillomavirus type 1 (bpv-1), hpv-18, hpv-11, it binds dna at the enhancer consensus motif accn6ggt. base and phosphate backbone contact points were determined using methylation protection and interference and ethylation interference as ... | 1990 | 2154890 |
| human papillomavirus type 6b dna required for initiation but not maintenance of transformation of c127 mouse cells. | we describe the transformation of c127 mouse fibroblasts with human papillomavirus type 6b (hpv-6b) dna, which is associated primarily with benign tumors of the human genital tract. the major transformed phenotype of the hpv-6b-transfected cells lines, which had been g418 selected, pooled, and maintained without subsequent selection, was tumorigenicity in nude mice. we found that, unlike that reported for other hpvs or papovaviruses, the transformed phenotype was expressed after a delay, in whic ... | 1990 | 2154622 |
| longitudinal study of human papillomavirus infection of the female urogenital tract by in situ hybridization. | the recurrence and/or persistence of human papillomavirus (hpv)-associated lesions constitute a well-recognized clinical problem; yet, few studies have demonstrated and typed the hpv within these recurrence lesions. a patient with a 17-year history of anogenital condylomata presented with an unusual bladder tumor that had histologic features of hpv infection. this prompted a longitudinal analysis of hpv dna in archival material from these urogenital lesions. colorimetric in situ hybridization wa ... | 1990 | 2154168 |
| anal cloacogenic and squamous carcinomas. comparative histologic analysis using in situ hybridization for human papillomavirus dna. | in order to evaluate the morphologic and possible etiologic distinctions between anal cloacogenic and squamous carcinomas, we performed histologic examination and in situ hybridization for human papillomavirus (hpv) dna on anal canal and anal verge carcinomas from 37 patients. twenty-one neoplasms were invasive or in situ squamous carcinomas, 14 were invasive cloacogenic carcinomas, and two were unclassified. in situ hybridization was positive for hpv types 16/18 in 12 cases and for types 6/11 i ... | 1990 | 2154141 |
| oral hairy leukoplakia. | oral hairy leukoplakia was first described in homosexual men infected with the human immunodeficiency virus. it is thought to be caused by infection with both the epstein-barr virus and human papillomavirus. we report 59 cases of oral hairy leukoplakia. the disease was diagnosed in patients in all risk groups and was categorized in all classes of the walter reed classification without significant differences in prevalence. epstein-barr virus could be demonstrated in all tissue samples examined; ... | 1990 | 2153716 |
| in situ hybridization analysis of human papillomavirus dna segregation patterns in lesions of the female genital tract. | various histologic features may be used to divide human papillomavirus (hpv)-related lesions of the genital tract into two groups: condylomata and "low-grade" or grade 1 cervical intraepithelial neoplasias (cin 1) versus "high-grade" or grade 2 and 3 intraepithelial neoplasias. using in situ hybridization analysis we correlated hpv dna type with histologic features in 350 biopsies of lesions from the cervix, vulva, and perianal region. hpv dna was most commonly found in vulvar and perianal condy ... | 1990 | 2153624 |
| human papillomavirus type 16 dna is integrated into chromosome region 12q14-q15 in a cell line derived from a vulvar intraepithelial neoplasia. | the sk-v cell line, established from a precancerous lesion (a vulvar intraepithelial neoplasia), contains 10 to 20 copies of the human papillomavirus type 16 (hpv16) genome, and was previously shown to derive from a clone of cells present in the patient's lesions. by in situ hybridization the integrated hpv16 dna sequences were localized to a single site in chromosome region 12q14-q15. the localization of viral sequences to a single nonrearranged chromosome 12 suggests that integration occurred ... | 1990 | 2153440 |
| human papillomavirus dna in co2 laser-generated plume of smoke and its consequences to the surgeon. | carbon dioxide laser energy is absorbed by intracellular water but not by proteins or nucleic acids. the possibility of dispersing viral dna during laser therapy of human papillomavirus (hpv)-containing genital infections was explored using a filter hybridization technique. samples were taken using dacron swabs from 110 patients in nine separate treatment sessions as well as from five pre-filter canisters, four fume vacuum tubes, and from the nasopharynx, eyelids, and ears of the laser surgeon b ... | 1990 | 2153274 |
| analysis of integrated human papillomavirus type 16 dna in cervical cancers: amplification of viral sequences together with cellular flanking sequences. | we have isolated four clones of integrated human papillomavirus type 16 (hpv-16) dna from four different primary cervical cancer specimens. all clones were found to be monomeric or dimeric forms of hpv-16 dna with cellular flanking sequences at both ends. analysis of the viral sequences in these clones showed that e6/e7 open reading frames and the long control region were conserved and that no region specific for the integration was detected. analysis of the cellular flanking sequences revealed ... | 1990 | 2153245 |
| the demonstration of human papillomavirus 16 genomes in the nuclei of genital cancers using two different methods of in situ hybridization. | biopsy specimens of 16 invasive genital cancers (two vulval carcinomas, two carcinomas of the vagina, and 12 cervix carcinomas) were examined for the presence and distribution of human papillomavirus (hpv) dna by in situ hybridization with 3h-labeled and biotinylated dna probes of hpv 6, hpv 11, and hpv 16. none of the tumors reacted with hpv 6 or hpv 11. using in situ hybridization with 3h-labeled dna probes nine of the 16 cancers gave positive results with hpv 16. only three of the nine were p ... | 1990 | 2153043 |
| mutational analysis of human papillomavirus type 16 e7 functions. | the human papillomavirus type 16 e7 gene encodes a nuclear oncoprotein (98 amino acids [aas] long) consisting of three regions: regions 1 (aas 1 to 20) and 2 (aas 21 to 40), which show high homology to the sequences of conserved domains 1 and 2, respectively, of adenovirus e1a; and region 3 (aas 41 to 98) containing two metal-binding motifs cys-x-x-cys (aas 58 and 91 to 94). we constructed aa deletion (substitution) mutants and single-aa substitution mutants of e7 placed under the control of the ... | 1990 | 2152813 |
| the nuclear oncoproteins: rb and p53. | a novel class of oncogene has been recognised whose loss-of-function results in the expression of the malignant phenotype. two examples of such genes are the human retinoblastoma predisposition gene (rb1) and the gene encoding the cellular protein p53. these genes are thought to regulate and limit normal proliferation of cells and, as a consequence, can suppress tumorigenicity when introduced into transformed cells. they are hence frequently described as 'tumour suppressor genes'. both rb1 and p ... | 1990 | 2151736 |
| sexually transmitted diseases in lesbians. | the limited published data support the assumption that lesbians are a low risk group for sexually transmitted diseases (stds). however, there are virtually no contemporary data and the older papers do not take into account genital wart virus infection, now the third most prevalent std in britain. one of the dangers in assuming that lesbians are a 'safe group' for std and allied disorders is that patients may not attend for regular cervical cytology and could be at risk of developing cervical can ... | 1990 | 2083290 |
| case-control study of risk factors for cervical neoplasia in denmark. i: role of the "male factor" in women with one lifetime sexual partner. | possible risk factors for cervical cancer were investigated in 645 women, 20-49 years of age from copenhagen, with histologically confirmed cervical cancer or carcinoma in situ and in 614 controls drawn at random from the female population of the same area. to study the role of the "male factor", monogamous cases and controls together with their husbands were invited for further examination. this included a personal interview, a blood sample for analysis of herpes simplex virus antibodies, and a ... | 1991 | 2019457 |
| cervicography for evaluating the "atypical" papanicolaou smear. | a study was designed to compare various case-finding procedures in the evaluation of patients with an "atypical" papanicolaou smear. six hundred eighty-one consecutive patients were referred to the denver general hospital colposcopy clinic for smears showing evidence of atypical nuclear and/or cytoplasmic changes insufficient to justify a cytologic diagnosis of dysplasia. a full colposcopic evaluation with appropriate directed biopsies was carried out on all the patients. fourteen percent of the ... | 1991 | 2010901 |
| immunohistochemical localization of glutathione s-transferase in preneoplastic and neoplastic lesions of the human uterine cervix. | a mouse monoclonal antibody and a rabbit polyclonal antibody prepared against the placental form of the enzyme glutathione s-transferase (gst-pi) were used to immunohistochemically stain normal and neoplastic human uterine cervical tissues from 88 cases. of 65 cases of preneoplastic squamous lesions and invasive carcinomas of the cervix, 94% stained with the monoclonal antibody and 100% with the polyclonal antibody. in the 23 benign tissues, staining of ectocervical squamous epithelium was gener ... | 1991 | 1994644 |
| immunological study of condylomata acuminata in men infected with the human immunodeficiency virus. | as condylomata acuminata often persist in individuals infected with the human immunodeficiency virus (hiv), an immunohistological study of warts from infected men was undertaken to further knowledge about human papillomavirus persistence in this group. using an indirect immunoperoxidase method and a panel of monoclonal antibodies, the phenotypes of cells were studied in cryostat sections of perianal or anal warts removed from 14 hiv-infected men (10 homosexual and 4 heterosexual) and from 16 non ... | 1990 | 1983071 |
| detection of human papillomavirus dna in human cervical lesions by tissue in situ nucleic acid hybridization. | cervical cancer is one of the most common female cancers in taiwan. certain types of human papillomavirus (hpv) are frequently detected in the epithelial precancerous and cancerous lesions of the cervix. by the use of tissue in situ hybridization, we investigated the relationship of various types of hpv (group i, hpv-6 & 11, group ii, hpv-16 & 18, group iii, hpv-31, 33 & 35) with cervical condyloma, carcinoma as well as precancerous lesions. group i hpv dnas were mainly found in cervical condylo ... | 1990 | 1982125 |
| anal human papillomavirus infection among human immunodeficiency virus-seropositive and -seronegative men. | homosexual men are at high risk of anorectal human papillomavirus (hpv) infection, hpv-related anal cancer, and precancer, conditions known to increase with immunosuppression. the relationship between anal hpv infection, human immunodeficiency virus (hiv) infection, and immunosuppression was studied in homosexual men seen at a sexually transmitted disease clinic. history or presence of warts on rectal examination, and detection of anorectal hpv dna were each significantly associated with hiv ser ... | 1990 | 1973695 |
| association between poor prognosis in early-stage invasive cervical carcinomas and non-detection of hpv dna. | human papilloma virus (hpv) dna sequences (hpv types 16, 18, 33, 35 or uncharacterized) were detected by southern blot hybridisation and polymerase chain reaction in 84% of 106 early-stage invasive carcinomas of the uterine cervix. among hpv-positive patients, the risk of overall relapse did not differ with individual hpv types. compared with hpv-positive patients, those with no detectable hpv dna had a 2.6 times higher risk of overall relapse (p less than 0.05) and 4.5 times higher risk of dist ... | 1990 | 1971033 |
| human papillomavirus oncoproteins. | human papillomaviruses (hpvs) appear to play a role in the etiology of the vast majority of virus-associated human malignancies. studies of viral gene expression in carcinomas suggest the importance of two hpv encoded proteins, e6 and e7, in malignant development and these proteins have been shown to encode transforming and immortalising activities. the two proteins show some functional resemblance to the transforming proteins of other small dna tumour viruses such as adenovirus and sv40. recent ... | 1990 | 1966493 |
| detection of human papillomavirus (hpv) in genital warts and carcinomas by dna in situ hybridization in chinese patients. | a series of 51 genital biopsies from normal epithelium, condylomata acuminata, leucoplakia and squamous cell carcinoma from chinese male and female patients were analysed for the presence of human papillomavirus (hpv) types 6, 11, 16 and 18 by dna in situ hybridization. all of the nine genital condyloma acuminata were positive for hpv dna, in which hpv 6 was found in six cases, hpv 11 in two cases and hpv 18 in one case. twelve out of the 21 cases (57.1% of the total) of cervical squamous cell c ... | 1990 | 1966324 |
| cryotherapy in the management of histologically diagnosed subclinical human papilloma virus (hpv) infection of the cervix. | the aim of the study was to establish the efficacy of cryotherapy in the management of histologically diagnosed subclinical human papilloma virus (hpv) infection of the cervix. women with genital warts who had subclinical hpv infection of the cervix and no other sexually transmitted diseases were followed up at six weeks, 12 weeks, six months, 12 months and 18 months after treatment with cryotherapy. they came to the out-patient department of genito-urinary medicine (gum) at st luke's hospital, ... | 1990 | 1966312 |
| [pathomorphology of human papillomavirus (hpv) infection of the cervix uteri and vulva]. | an infection with human papilloma virus (hpv) is directly associated with the development of the cervical and vulvar carcinomas. the infection should be diagnosed and considered in the choice of therapy. taking no account of hpv infection in histological pictures may lead to overdiagnosis of ca"0" (cis). hpv infection is possible to be recognized--in most cases--in cytological and histopathological studies. cytological and histopathological studies are the basis for other examination methods. th ... | 1990 | 1966114 |
| genital human papillomavirus infection among women from major ethnic groups in singapore. | the close epidemiological relationships between specific genital human papillomavirus (hpv) types and neoplasia of the cervix uteri have been extensively documented worldwide, including singapore. cervical cancer incidence rates in singapore show variations between the major ethnic groups. to ascertain the corresponding hpv infection rates among the various races in singapore, we analysed the cervical smears of 225 women by filter in situ dna hybridization, and compared the data with a previous ... | 1990 | 1966000 |
| detection of hpv-16 dna in cervical carcinoma by paraffin section in situ hybridization. | thirty-eight cervical carcinomas and 1 cervical condyloma were examined for the presence of human papillomavirus (hpv) type 16 dna using 3h-dctp-labelled hpv-16 dna probe in paraffin section in situ hybridization and 32p-dctp-labelled probe in dot blot hybridization (tm-17). the results showed an hpv-16 dna positive rate in our series of about 74.4% (29/39) as determined by paraffin section in situ hybridization and about 71.8% (28/29) by dot blot hybridization. there was no significant differen ... | 1990 | 1965917 |
| [penile lesions induced by human papillomavirus. an immunohistochemical study and dna hybridization]. | forty-nine male patients with clinically evident genital warts or suspected hpv-induced lesions detected by 5% acetic acid test were biopsied. typical cytological and structural hpv-induced changes were observed on histological examination in 37 of 49 patients. the immunohistochemical detection of hpv capsid antigen was positive in 15 of 45 cases. in situ hpv-dna hybridization was positive in 26 of 47 cases (24 hpv 6/11; 2 hpv 16/18). quick blot hpv-dna hybridization was positive in 20 of 28 cas ... | 1990 | 1965182 |
| immunology of anogenital human papillomavirus (hpv) infection. | a subgroup of human papillomaviruses (hpvs) is implicated in the aetiology of neoplastic change in anogenital epithelium. although abundant circumstantial evidence exists for the involvement of the immune system in the control of hpv infection, restriction of infection to epithelium and the lack of a viral productive phase pose problems for immune response induction, and for immunological effector mechanisms. in this article we discuss how hpv antigens may be presented to the immune system on th ... | 1990 | 1964551 |
| a comparative sequence analysis of two human papillomavirus (hpv) types 2a and 57. | hpv 2a is commonly associated with verrucae vulgares, whereas hpv 57 was detected in mucosal lesions of the maxillary sinus and the genital tract, as well as in cutaneous lesions. the complete dna sequences of hpv 2a and hpv 57 were determined. the hpv 2a genome consists of 7860 base pairs and the hpv 57 genome contains 7861 base pairs. on the nucleotide level an 83% homology between the two sequences could be ascertained. compared to other hpvs they have a high g/c-content (hpv 2a: 48.8%, hpv 5 ... | 1990 | 1964523 |
| precursor lesions of the cervix. | screening has played a major role in decreasing the morbidity and mortality associated with carcinoma of the cervix. aggressive management of lesions recognized as precursor to invasive squamous carcinoma, (cervical intraepithelial neoplasia), has markedly reduced the incidence of invasive cancer. squamous carcinomas constitute most of the cervical cancers. the relationship between human papilloma virus and cervical abnormalities is reviewed. nursing diagnoses and care planning ideas are discuss ... | 1990 | 1964388 |
| human papillomavirus dna from warts for typing by endonuclease restriction patterns: purification by alkaline plasmid methods. | the alkaline plasmid dna extraction method of birnboim and doly was applied for the isolation of human papillomavirus (hpv) from warts. tissue from common and plantar warts was digested with proteinase k, and the extrachromosomal circular covalently-closed form of hpv-dna was rapidly extracted by alkaline sodium dodecyl sulphate and phenol-chloroform treatment. recovery of hpv-dna from the tissue was sufficient for determination of endonuclease restriction patterns by agarose gel electrophoresis ... | 1990 | 1964033 |
| [relationship between human papillomavirus and oncogenes (c-myc, n-myc) amplification in human cervical cancers]. | the human papillomavirus detection and oncogenes amplifications were studied on dnas from fifteen cervical cancers. we detected hpv16 and hpv18 using southern blot hybridization and polymerase chain reaction (pcr) technique. the positive subjects of hpvs were eight cases (53%) observed by southern blot hybridization and fourteen cases (93%) by pcr technique. the gene amplifications of oncogenes (c-myc and n-myc) were analysed by slot-blot method and were observed in c-myc but not in n-myc. the " ... | 1990 | 1963632 |
| discrimination of closely homologous hpv types by nonisotopic in situ hybridization: definition and derivation of tissue melting temperatures. | it is generally assumed that nucleic acid association during in situ hybridization reactions is similar to that of nucleic acid association in solution. this assumption has been investigated by detecting closely homologous human papillomavirus types 6 and 11 by in situ hybridization as a model for the evaluation of stringency conditions in clinical biopsies. by examining matched and mismatched, labelled and target sequences under various stringency conditions, empirical dna-dna stability curves ... | 1990 | 1963174 |
| herpes simplex and human papillomavirus genital infections: controversy over obstetric management. | plasma inhibitory factors, high levels of sex hormones, and depression of cell-mediated immunity may interfere with the natural host resistance to viral infections during pregnancy. it is apparent that hormonal, immunologic, and vascular changes in pregnancy may account for increased replication of herpes and for enhanced growth of condylomatous lesions. the challenge is to develop a rational plan of management for pregnant patients with herpes simplex or human papilloma virus infection. there h ... | 1990 | 1963124 |
| transcriptional activation of the human papillomavirus-16 p97 promoter by an 88-nucleotide enhancer containing distinct cell-dependent and ap-1-responsive modules. | the p97 promoter upstream of the oncogenic early genes of human papillomavirus (hpv)-16 is active in keratinocytes and in cervical carcinoma cells due to a 5' keratinocyte-dependent cis enhancer. in this study, we have mapped the main enhancer activity to an 88-nucleotide (nt) fragment composed of multiple cis elements. a 63-nt promoter-proximal enhancer core was sufficient for p97 activation in a human keratinocytic cell line, hacat, and in cervical carcinoma cells. although the enhancer functi ... | 1990 | 1963084 |
| hybricomb. a novel diagnostic tool for dna probing. | a technique is described in which unlabeled dna probes are immobilized on the plastic surface of a 12-tooth comb and used to capture homologs by a hybridization procedure. in the examples described, cellular dna from cervical biopsies is chemically labeled using the chemi-probe system. the sample containing labeled dna is then hybridized with the immobilized unlabeled probe (reverse hybridization). by means of this technique, human papillomavirus sequences can be detected with a sensitivity comp ... | 1990 | 1963055 |
| [in situ hybridization study of human papillomavirus from the penile cancer]. | the histochemical detection of human papillomavirus (hpv) was performed using the technique of in situ hybridization from the formalin fixed paraffin embedded specimens of penile carcinoma and condylomata+ acuminata . one of 19 penile cancer cases (5.3%) revealed the positive staining on the nuclei of the tumors for hpv type 16/18. however, the positive nuclei were scattered and localized in small part of the tumor which showed no koilocytosis. on the contrary, 11 of 12 condyloma acuminata cases ... | 1990 | 1962829 |
| [studies of human papillomavirus (hpv) in urological tumors]. | urological tumors were examined for the presence of human papillomavirus (hpv) dna by using southern blot hybridization. in 20 male patients with condyloma acuminatum, hpv type 6 was found at 85% (17/20), hpv type 11 at 95% (19/20), hpv type 16 at 5% (1/20) and hpv type 18 at 0% (0/20). in 2 female patients with condyloma acuminatum, hpv types 6, 11, 16 and 18 were found at 100% (2/2), 100% (2/2), 50% (1/2) and 0% (0/2), respectively. all 6 of the patients who were positive for hpv type 6, were ... | 1990 | 1962828 |
| clinical and pathological correlates of hpv type 16 dna in anal cancer. | the aetiology of anal squamous cell carcinoma (scc) has recently been associated with a sexually transmissible agent--human papillomavirus (hpv) type 16. in this study clinical and pathological data from a prospective series of 67 anal scc collected over a three year period were compared with the hpv type 16 dna content of these tumours to determine whether any of the clinical or histological parameters might predict hpv dna content in a tumour specimen. of twelve clinicopathological variables e ... | 1990 | 1962812 |
| general sexually transmitted diseases. | this review discusses current trends in sexually transmitted diseases. topics include treatment and prevention of syphilis and gonorrhea, as well as the association of sexually transmitted diseases with sexual assault. also discussed are the various organisms that cause vulvitis, including trichomonas, candida, and gardnerella species, along with a discussion of vulvar lesions that are especially difficult to diagnose and treat. diagnosis, sampling, pathogenesis, and treatment of chlamydia trach ... | 1991 | 1958806 |
| cervico-vaginal screening in an std clinic. | from may 1986 to march 1988, there were 3,622 "new" female clients at the calgary sexually transmitted disease (std) clinic of whom 2,278 registered for the first time. a cervico-vaginal (pap) smear was obtained from those who had not had one in the previous 6 to 12 months and any history of venereal warts (vw) was recorded. 621 smears were accessed of which 611 were suitable for inclusion in this study. 65 (10.6%) smears revealed human papillomavirus (hpv) and/or cervical intraepithelial neopla ... | 1991 | 1954594 |
| warts: benign or malignant? | the number of identifiable types of human papillomavirus (hpv), based on deoxyribonucleic acid (dna) sequence, has steadily increased in recent years. although at one time verruca vulgaris was considered a benign reactive proliferation, current evidence submits that this is not the case; some types of hpv are malignant. | 1991 | 1940290 |
| an ounce of prevention ... stds and women's health. | using recent data from the u.s., the argument is reiterated that sexually transmitted diseases (stds) have serious consequences on female morbidity, mortality, pregnancy outcome and that the cost of screening and treatment can afford significant savings. since the rosy view of the 1950s that stds had been eliminated with penicillin, new viral, parasitic and bacterial forms have emerged, and antibiotic-resistant classical stds have increased yearly. 13 million new cases were reported in 1989, e ... | 1991 | 1936219 |
| human papillomavirus, gonorrhea, syphilis, and cervical dysplasia in jailed women. | we assessed the prevalence of human papillomavirus (hpv) by cervicovaginal lavage and southern blot and inquired about behavioral risk factors for cervical disease and sexually transmitted diseases by interview in 114 female detainees at a large new york city jail. of the women screened, 8% had abnormal pap smears, 35% had hpv, 7% had gonorrhea, and 22% had serologic syphilis. given the high rates of hpv infection and cervical cytology, pap smears should be a routine intake procedure for incarce ... | 1991 | 1928533 |
| [spontaneous regression of flat condylomata of the cervix]. | in this study 146 patients with a human papilloma virus infection of the cervix detected on cervical smear. this infection was at the worst associated with cin 1 or cin 2. no treatment was carried out on these patients except for advising the use of condoms. among the 123 women whose diagnosis was cin 1, 65.2% of them had a negative smear within 18 months. this somewhat high rate of spontaneous regression was found in out patients who were not selected. they are near the results published by bro ... | 1991 | 1885888 |
| hiv and other sexually transmitted diseases among female prostitutes in kinshasa. | in 1988, 1233 prostitutes from different geographic areas of kinshasa participated in a cross-sectional survey on hiv infection and other sexually transmitted diseases (stds). despite relatively good knowledge about aids and stds, the reported preventive behaviour was poor. only 12% of the women reported regular use of condoms, while greater than 50% of the women reported regular use of antibiotics and 38% reported doing nothing specific to prevent stds. thirty-five per cent of the women were hi ... | 1991 | 1883543 |
| human papillomavirus infection and other risk factors for cervical neoplasia: a case-control study. | a case control design has been used to investigate risk factors associated with the development of cervical squamous intraepithelial lesions (sil) in a population of urban women in which non-affluent minority groups were heavily represented. eighty-five women with histologically confirmed sil were compared to a control group of 70 cytologically normal women. hpv infection was determined using both southern blot hybridization and polymerase chain reaction (pcr) amplification specific for hpv type ... | 1991 | 1874571 |
| cervical human papillomavirus deoxyribonucleic acid and cytologic evaluations in gynecologic outpatients. | we studied the prevalence and cytologic manifestations of human papillomavirus deoxyribonucleic acid among 2668 gynecologic outpatients. the study population consisted of 1573 women who came into the emergency room, 623 women from an abortion clinic who were seen because of induced first-trimester abortion, and 472 women who had been referred to a colposcopy clinic because of an abnormal papanicolaou smear finding. a dot blot technique was used for the detection of human papillomavirus types 6/1 ... | 1991 | 1852101 |
| the detection of genital human papillomavirus infection using polymerase chain reaction. | 1991 | 1851893 | |
| human papillomavirus screening in college women. | 1991 | 1851770 | |
| correlation of hpv antigen and type of genital warts with atypia. | five clinical types of genital warts were identified, studied histopathologically and stained for human papilloma virus (hpv) antigen. hpv antigen was found in all degrees of koilocytosis and it was present more often in the hyperplastic warts than in other types of genital warts. however, as reported earlier, the presence of hpv antigen did not decrease proportionately as the degree of atypia increased. | 1991 | 1851721 |
| prevalence of different types of human papillomavirus in cervical infection of north italian women. | we investigated the prevalences of human papillomavirus (hpv) of type 6, 11, 16, 18, 31, 33, 35 and 42 in 276 cervical tissue samples biopsied from a group of north-italian women in which a hpv infection of the cervix had been suspected. the specimens were evaluated by conventional histological examination and by hpv typing, using southern blot hybridization on the extracted dna. we related different degrees of squamous cervical dysplasia, showing or not viral cytopathic effects, with the hpv ty ... | 1991 | 1851706 |
| human papillomavirus types 16 and 18 in adenocarcinoma of the uterine cervix. | many reports have shown a link between human papillomavirus (hpv) and cervical squamous neoplasia. however, the association of hpv with cervical adenocarcinoma has been studied less extensively. the authors evaluated the presence of hpv-dna in 106 patients with adenocarcinoma of the uterine cervix by in situ hybridization, using 35s-labeled probes for hpv 16 dna and hpv 18 dna. the overall prevalence of hpv-dna was 18% (19 of 106). hpv 16 was present in 2 (2%) cases, hpv 18 was observed in 15 (1 ... | 1991 | 1850951 |
| diagnostic sensitivity of polymerase chain reaction and southern blot hybridization for the detection of human papillomavirus dna in biopsy specimens from cervical lesions. | human papillomaviruses (hpvs) are associated with benign and malignant neoplasms of the cervix. one of the criteria for their etiologic role requires an assessment of whether virtually all or only a small fraction of lesions contain viral genomes. dna preparations from colposcopically directed punch biopsies of cervical lesions were analyzed by southern blot hybridization and the polymerase chain reaction (pcr) for the presence of hpv dna. the biopsy specimens represented different pathologic en ... | 1991 | 1850950 |
| expression of human papillomavirus proteins in yeast saccharomyces cerevisiae. | the l1 and l2 proteins of human papillomavirus (hpv) types 1, 6, and 16 and the e6 and e7 proteins of hpv 16 were expressed in saccharomyces cerevisiae. the yeast expressed proteins were readily detected by immune blotting and were generally intact. the hpv 1 l1 and l2 proteins expressed in yeast were indistinguishable from the major and minor capsid proteins purified from hpv 1 virions as judged by gel electrophoresis and immunoblotting. the hpv 6 and hpv 16 l2 proteins and hpv 16 e7 proteins w ... | 1991 | 1850917 |
| human papillomavirus infection among hiv-infected individuals. implications for development of malignant tumors. | human papillomavirus (hpv) infection has been strongly associated with development of anogenital squamous cell cancer. cell-mediated immunity likely plays an important role in prevention of hpv-associated disease, and hpv-associated squamous cell cancer has been shown to occur with increased frequency among iatrogenically immunosuppressed individuals. similarly, individuals with hiv-associated immunodeficiency have been shown to have a high prevalence of anogenital hpv infection as well as a hig ... | 1991 | 1850727 |
| comparison of peroxidase-labeled dna probes with radioactive rna probes for detection of human papillomaviruses by in situ hybridization in paraffin sections. | a study comparing in situ hybridization using nonradioactive dna probes directly conjugated with horseradish peroxidase (hrp), and 35s-labeled antisense rna probes for human papillomavirus (hpv) types 6/11, 16, and 18 was performed on formalin-fixed, paraffin-embedded tissue from 34 lesions of the cervix and vulva. these lesions included exophytic condylomas and intraepithelial and invasive neoplasms. hpv 6/11 was detected in two of four condylomata acuminata by both in situ techniques. hpv 16 w ... | 1991 | 1850519 |
| human papillomavirus detection in adenocarcinoma of the anus. | human papillomavirus (hpv) is associated with carcinoma of the cervix but not with carcinoma of the endometrium. hpv 16 is the type most commonly detected in squamous cell carcinomas from this site, whereas hpv 18 predominates in adenocarcinomas. we analyzed eight anal carcinomas for hpv dna using the polymerase chain reaction and type-specific (open reading frame e6) primers for hpv 16, 18, 31, and 35. hpv dna sequences were amplified in two of six anal adenocarcinomas and, in each case, the ty ... | 1991 | 1850518 |
| focal epithelial hyperplasia (heck's disease): the first reported case from italy. | a case of focal epithelial hyperplasia (heck's disease) of the oral mucosa observed for the first time in italy is reported. the patient was of italian extraction. the lesions, represented by soft nodules, were multiple and located on the vestibular and labial mucosa. biopsy tissues were studied for the presence of human papilloma virus (hpv) by electron microscopy, in situ hybridization and immunoperoxidase staining to hpv group antigens. no viral particles consistent with hpv were found in the ... | 1991 | 1850180 |
| amplification of human papillomavirus genomes in vitro is dependent on epithelial differentiation. | human papillomaviruses (hpvs) infect squamous epithelium and establish their genomes as episomes in proliferating basal cells. as infected cells differentiate, the viral dna is amplified to high copy number and infectious virus is produced. viral production has not yet been observed in vitro due to the inability of standard culture methods to duplicate most stages of epithelial differentiation. in this study, we have examined a cell line derived from a low-grade cervical lesion and found that it ... | 1991 | 1850010 |
| presence and distribution of human papillomavirus sense and antisense rna transcripts in genital cancers. | rna transcription in eight human papillomavirus (hpv) type 16-positive genital carcinomas and in two cervical squamous cell carcinoma (scc)-derived cell lines was analysed by in situ hybridization using 125i-labelled subgenomic riboprobes. transcripts corresponding to the e6 and e7 open reading frames were always present, except within the keratinizing layers of differentiated sccs. intranuclear e1 gene transcripts were detectable in both cell lines and some tumours whereas e2/e4 transcripts wer ... | 1991 | 1849971 |
| the use of the polymerase chain reaction for the detection of human papillomavirus type 13. | human papillomavirus type 13 (hpv-13) is associated with oral focal epithelial hyperplasia (feh). the purpose of this study was to establish conditions for the application of polymerase chain reaction (pcr) to the specific detection and amplification of hpv-13 dna. to design primers for hpv-13 a part of the hpv-13 genome was sequenced first: the smallest bamhi fragment (597 bp) of hpv-13 was subcloned and sequenced. the sequence was found to be part of a large open reading frame and had signific ... | 1991 | 1849917 |
| cervical papillomavirus infection and cervical dysplasia in hispanic, native american, and non-hispanic white women in new mexico. | human papillomavirus infections of the cervix are found with varying frequencies in different populations worldwide, and have been associated with cervical cytologic abnormalities. | 1991 | 1849706 |
| human papillomavirus segregation patterns in genital and nongenital warts in prepubertal children and adults. | this study compared the segregation patterns of human papillomavirus (hpv) in genital and nongenital warts in prepubertal children and adults. hpv 2 was detected in most nongenital warts in children and adults, whereas neither hpv 6 or 11 was detected at nongenital sites in either group with the use of in situ or southern blot hybridization analyses. of nine genital tract lesions in children. hpv 2 was detected in two and hpv 6 or 11 in six. more than 90% of cases of regional tract condylomata i ... | 1991 | 1849698 |
| from papanicolaou to bethesda: the rationale for a new cervical cytologic classification. | the bethesda system for reporting cervical/vaginal diagnoses was introduced to replace the numerical papanicolaou class designations, thereby facilitating precise communication between cytopathologist and clinician. the terminology for squamous epithelial lesions includes the following categories: 1) atypical squamous cells of undetermined significance; 2) squamous intraepithelial lesion (sil), which encompasses the spectrum of squamous cell carcinoma precursors, divided into low-grade sil (huma ... | 1991 | 1849626 |
| amino acid sequence homology of epstein-barr virus nuclear antigen-2 to adenovirus e1a, human papilloma virus-16 e7 and sv40 large t antigen in the functional domains for growth transformation. | 1991 | 1849247 | |
| chromosomal characterizations of human nasal and nasopharyngeal cells immortalized by human papillomavirus type 16 dna. | cell lines nw-1 and npc-n were established by transfection of human nasal and nasopharyngeal epithelial cells, respectively, with human papillomavirus type 16 (hpv-16) dna. additional treatment of npc-n cells with mitomycin c gave rise to the cell line npc-n-m. chromosomal analysis showed that 40-47% of nw-1, npc-n, and npc-n-m cells had a near-tetraploid number of chromosomes and several chromosomal abnormalities. these cells showed evidence of gene amplification in the form of double minutes ( ... | 1991 | 1849038 |
| bowenoid papulosis. | bowenoid papulosis is an uncommon genital dysplasia induced by human papillomavirus infection. clinically, it usually resembles persistent warts, but histologically it may be suggestive of squamous cell carcinoma in situ. this unusual disorder and recent advances in our understanding of it are reviewed. | 1991 | 1848870 |
| evidence of human papillomavirus deoxyribonucleic acid in vulvar squamous papillomatosis. | squamous papillae of the vulva represent a controversial lesion. forty-six women with histologically confirmed vulvar squamous papillae and koilocytosis were studied at chang gung memorial hospital. vulvar washings were analyzed by the filter in-situ technique using biotin-labeled probes for typing human papillomavirus dna. forty of 46 women (87%) were symptomatic. human papillomavirus (hpv) dna was detected in 76% (35 of 46) of women, including type 6/11 in 21.7%, type 16/18/31/33 in 45.6%, and ... | 1991 | 1848835 |
| subclinical manifestations of vulvar human papillomavirus infection. | we studied the presence of human papillomavirus (hpv) dna by in situ hybridization in 74 colposcopically defined subclinical vulvar lesions obtained from 51 patients (mean age 25 years, range 18-42 years) referred for atypical papanicolaou smears to the colposcopy clinic. serial sections of formalin-fixed, paraffin-embedded biopsy tissue were stained by routine hematoxylin and eosin stain or were separately hybridized with biotin-labeled probes of hpv types 6, 11, 16, and 18 at high stringency a ... | 1991 | 1848833 |
| prevalence of human papillomavirus genomes in tissues from the lower genital tract as detected by molecular in situ hybridization. | incidence cases of human papillomavirus (hpv)-related disease of the lower anogenital tract were prospectively collected and submitted to rna-dna in situ hybridization, which allows a direct correlation between morphology and the presence of viral dna. the overall hpv detection rate in condylomata and intraepithelial or invasive neoplasia varied for the different genital areas: cervix (64%), vagina (56%), vestibule (59%), vulva (50%), and penis (42%). hpvs 6/11 were associated with condylomata, ... | 1991 | 1848832 |
| human papillomavirus type 1-associated squamous cell carcinoma in a heart transplant recipient. | the occurrence of squamous cell carcinomas in organ transplant recipients with warts represents a good model to study viral carcinogenesis. most of the cases were reported in renal transplant recipients. we present the case of a heart transplant recipient in whom multiple common warts, preepitheliomatous keratoses, and squamous cell carcinomas developed. the warts began 4 years after the transplantation and the first carcinoma occurred 2 years after the warts, all the lesions being on sun-expose ... | 1991 | 1848746 |
| high prevalence of human papillomavirus in prostate tissues. | specific human papillomavirus (hpv) types are associated with benign and malignant lesions of the anogenital region including the prostate gland. using polymerase chain reaction (pcr) amplification of type-specific hpv sequences, we have assessed the prevalence of hpv dna in prostate tissue from 88 individuals. amplified sequences specific for hpv 16 were found in 34 of 56 benign prostatic hyperplasias and in 14 of 27 prostatic carcinomas. in contrast, hpv 18 was identified in only three benign ... | 1991 | 1848641 |
| human papillomavirus types 16 and 33, herpes simplex virus type 2 and other risk factors for cervical cancer in sichuan province, china. | cancer of the cervix is relatively common in china, but has been investigated epidemiologically in only a few studies. in the hospital-based case-control study reported here, we investigated the role of various lifestyle and dietary factors, as well as infection with human papillomavirus (hpv) types 16 and 33 and herpes simplex virus type 2 in the aetiology of invasive cervical cancer. the study was conducted in sichuan province, and involved 101 cases with histologically-confirmed cervical canc ... | 1991 | 1848537 |
| epidermal growth factor (egf) elicits down-regulation of human papillomavirus type 16 (hpv-16) e6/e7 mrna at the transcriptional level in an egf-stimulated human keratinocyte cell line: functional role of egf-responsive silencer in the hpv-16 long control region. | we have found that epidermal growth factor (egf) elicits negative regulation of human papillomavirus type 16 (hpv-16) e6/e7 at the mrna level in the hpv-16-immortalized human keratinocyte cell line (phk160b). this down-regulation of hpv-16 e6/e7 expression was achieved when the cells were stimulated to proliferate with the concomitantly enhanced c-myc expression by egf in a dose-dependent manner. by using partly synchronized phk160b cells, negative and positive regulations of the hpv-16 e6/e7 ex ... | 1991 | 1848315 |
| oligonucleotide primers for dna amplification of the early regions 1, 6, and 7 from human papillomavirus types 6, 11, 16, 18, 31, and 33. | human papillomavirus (hpv) type-specific sequences required for polymerase chain reaction (pcr) mediated amplification of hpv dna sequences are presented. one primer pair within the e1 open reading frame (orf) was shared by hpv 6, hpv 11, hpv 16, and hpv 31, whereas the other primer pair within the e1 orf was specific for hpv 16. eight primer pairs from the e6 and e7 orfs specifically detected hpv 6, hpv 16, hpv 18, and hpv 33 sequences. this system has been used for detection of hpv dna in biop ... | 1991 | 1848065 |
| expression of anionic glutathione s transferase (gst pi) gene in carcinomas of the uterine cervix and in normal cervices. | the aim of the present study was to analyse in invasive carcinomas of the uterine cervix, the anionic glutathione s transferase (gst pi) gene, possibly implicated in the drug resistance of human cancers. total rna preparations obtained from invasive cervical cancers (106 specimens), carcinomas in situ (cis) (three specimens) and normal cervical epitheliums (24 specimens) were analysed by northern and slot blot hybridisation. a 0.7 kb gst pi transcript band was detected in all the cervical specim ... | 1991 | 1847644 |
| immunization against human papillomavirus type 16 tumor cells with recombinant vaccinia viruses expressing e6 and e7. | papillomaviruses are etiological agents of epithelial proliferative disease. in man, neoplastic transformation of the uterine cervix has been linked to infection with specific subtypes of human papillomavirus, particularly types 16 and 18. we previously reported that live vaccinia virus recombinants expressing early transforming proteins of other tumor viruses can immunize against challenge with cognate tumor cells and we have extended this approach to hpv16. neoplastic transformation by papillo ... | 1991 | 1847269 |
| human papillomavirus dna in biopsies of oral tissues. | the dnas of human papillomavirus (hpv) types 4, 16 and 18 have been detected in biopsies of normal and malignant human oral mucosa by southern blot hybridization and the polymerase chain reaction (pcr). by the former technique, hpv-4, hpv-16 and hpv-18 dnas were detected in three separate carcinomas but were found in adjacent dysplastic and normal tissue by the pcr only. the pcr technique also allowed detection of hpv-16 and hpv-18 dna in additional carcinomas and normal samples. the oral hpv-4 ... | 1991 | 1846646 |
| comparative virologic studies of condylomata acuminata reveal a lack of dual infections with human papillomaviruses. | condylomata acuminata are epithelial proliferations caused by infection of the anogenital squamous epithelium with human papillomavirus (hpv). dna-dna hybridization techniques and the extremely sensitive polymerase chain reaction (pcr) were used to analyze biopsies from patients with clinically diagnosed condyloma acuminatum for the presence of hpv dna. pcr analyses using primers and oligonucleotide probes specific for the e6/e7 region of hpv-6, -11, or -16 showed that 31 (93.9%) of 33 tissue bi ... | 1991 | 1846393 |
| detection of human papillomavirus dna in anal intraepithelial neoplasia and anal cancer. | forty anal paraffin-embedded tissue specimens from 24 subjects were studied for the presence of human papillomavirus (hpv) types 6, 11, 16, 18, 31, and 33, herpes simplex virus (hsv), epstein-barr virus, and cytomegalovirus dna by using the polymerase chain reaction. these tissues ranged from histologically normal to invasive squamous cell carcinoma. hpv dna was detected in the invasive anal cancer tissues of 11 of 13 subjects. hpv types were segregated by histopathological severity, with hpv 16 ... | 1991 | 1846314 |
| human immunodeficiency virus-induced immunosuppression: a risk factor for human papillomavirus infection. | in a group of 92 women with genital condylomata, 15 (16.3%) human immunodeficiency virus-positive patients were found, whereas no case was detected in a control group of 100 women. the relative risk was greater than 19.28. human immunodeficiency-positive status was associated with other parameters: lower age and parity, major frequency of induced abortions, and sexually transmitted diseases. thus although human immunodeficiency-positive status seems to be a true risk factor in relation to the al ... | 1991 | 1846062 |
| genital human papillomavirus infection in female university students as determined by a pcr-based method. | the presence of genital human papillomavirus (hpv) was determined at cervical and vulvar sites using two methods, the food and drug administration-approved virapap test and polymerase chain reaction (pcr) dna amplification technology, in 467 women presenting to a university health service for a routine annual gynecologic examination. the pcr system afforded the sensitive detection of a broad spectrum of genital hpv types. using pcr, we found that 46% of the study population was infected with hpv ... | 1991 | 1845912 |
| the e7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. | the contribution of the e6 and e7 open reading frames of human papillomavirus type 6b (hpv6b) and hpv16 to immortalization of human keratinocytes was evaluated by using amphotropic recombinant retroviruses. the hpv16 e7 gene could immortalize primary human keratinocytes without the cooperation of the viral e6 gene; however, e6 was able to contribute significantly to the efficiency of the e7 immortalizing function. infection of hfe cells with retroviruses carrying the 16e6, 6be6, or 6be6e7 open r ... | 1991 | 1845902 |
| condylomata acuminata and risk of cancer: an epidemiological study. | to determine whether patients with condylomata acuminata have an increased risk of developing cancer. | 1991 | 1781836 |
| a case-control study of nutrient status and invasive cervical cancer. i. dietary indicators. | a study of 748 cases and 1,411 hospital and community controls in four latin american countries evaluated the association between certain elements of diet and invasive cervical cancer. subjects were interviewed about their adult consumption of 58 food items, including the major sources of putative protective agents (vitamin a, carotenoids, vitamin c, and folacin) as well as other behavioral and medical characteristics related to cervical cancer. participation rates were above 95% for both cases ... | 1991 | 1755447 |
| [demonstration and biological significance of viral and cellular oncogenes in uterine carcinomas]. | premalignant and malignant lesions of the cervix uteri and endometrium were analyzed for the presence of human papillomavirus (hpv) dna and c-myc or c-erbb2/neu oncogene expression. hpv dna was detected by pcr in 100% of the cervical carcinomas and cin lesions, but also in endometrial lesions (3/8 adenocarcinomas, two hyperplasias and one adenomyosis uteri). myc-overexpression was found in 25-30% of the cervical carcinomas and severe dysplasias, but not in endometrial lesions. c-erbb2 was overex ... | 1991 | 1724842 |
| human papillomavirus type 18 e6 and e7 antibodies in human sera: increased anti-e7 prevalence in cervical cancer patients. | antibody-reactive regions on the human papillomavirus type 18 (hpv-18) e6 and e7 proteins were identified with rabbit polyclonal anti-fusion protein sera by screening of an fd phage expression library containing subgenomic hpv-18 dna fragments and by testing of overlapping decapeptides representing the e6 and e7 open reading frames. peptides comprising the delineated regions (designated e6/1 to e6/4 and e7/1) were synthesized and used in an enzyme-linked immunosorbent assay (elisa) to detect ant ... | 1991 | 1722219 |
| evaluation of histologic, morphometric, and immunohistochemical criteria in the differential diagnosis of small cell carcinomas of the cervix with particular reference to human papillomavirus types 16 and 18. | clinicopathologic analyses including immunohistochemical, morphometric, virologic, and dna ploidy studies were performed on seven cases of small cell (undifferentiated) carcinoma (scc) and 13 cases of small cell squamous carcinoma (scsc) of the uterine cervix in an attempt to evaluate which criteria are the most useful in identifying aggressive cervical carcinomas composed of small cells. highly malignant behavior was found to correlate most closely with the histologic pattern of the tumor. diff ... | 1991 | 1722042 |
| antigenic and immunogenic epitopes shared by human papillomavirus type 16 and bovine, canine, and avian papillomaviruses. | all types of papillomaviruses (pv) share common, so-called group-specific epitopes. to identify the major group-specific epitopes, we immunized 26 guinea pigs or rabbits with purified bovine pv type 1 (bpv), canine pv, or avian pv from the common chaffinch. the resulting hyperimmune sera, as well as a commercially available rabbit antiserum to bpv and seven monoclonal antibodies to bpv, were tested in an enzyme-linked immunosorbent assay with a set of 66 overlapping 20-amino-acid peptides repres ... | 1991 | 1719234 |
| human papillomavirus dna in tissue biopsy specimens of vulvar vestibulitis patients treated with interferon. | thirteen women with the diagnosis of vulvar vestibulitis based on clinical symptoms, presence of vestibular tenderness on physical examination, and acetowhite changes of the vulvar vestibule were treated with intradermal injection of alpha-interferon. biopsies of the acetowhite areas were analyzed for human papillomavirus (hpv) dna using polymerase chain reaction amplification and dot blot hybridization. eleven of 13 subjects harbored one of the hpv dna types; six of these were type 16 and/or 18 ... | 1991 | 1717908 |
| antibodies specific for the human retinoblastoma protein identify a family of related polypeptides. | even though the retinoblastoma gene is one of the best-studied tumor suppressor genes, little is known about its functional role. like all tumor suppressor gene products, the retinoblastoma protein (prb) is thought to inhibit some aspect of cell proliferation. it also appears to be a cellular target of several dna tumor virus-transforming proteins, such as adenovirus e1a, human papillomavirus e7, or simian virus 40 large t antigen. to help in the analysis of prb, we have prepared a new set of an ... | 1991 | 1717832 |
| specific interaction between hpv-16 e1-e4 and cytokeratins results in collapse of the epithelial cell intermediate filament network. | the human papillomaviruses (hpv) are associated specifically with epithelial lesions, ranging from benign warts to invasive carcinoma. the virus encodes three late proteins, which are produced only in terminally differentiating keratinocytes, two of which are structural components of the virion. the third, e1-e4, is derived primarily from the e4 open reading frame, which represents a region of maximal divergence between different hpv types. e1-e4 does not seem to be a component of the virus part ... | 1991 | 1715519 |
| expression of beta 4 integrins in human skin: comparison of epidermal distribution with beta 1-integrin epitopes, and modulation by calcium and vitamin d3 in cultured keratinocytes. | the expression of beta 4 integrins in adult and fetal human skin as well as in cultured keratinocytes was studied by immunodetection with monoclonal antibodies, and compared with that of beta 1 integrins. the distribution of the beta 1 and beta 4 integrin epitopes was entirely different in the adult epidermis. as reported previously by us (j clin invest 84:1916, 1989), the beta 1 epitopes were present most prominently at lateral cell-cell contact points, whereas staining with the beta 4 antibody ... | 1991 | 1714931 |
| identification of t- and b-cell epitopes of the e7 protein of human papillomavirus type 16. | there is strong evidence implicating human papillomavirus type 16 (hpv16) in the genesis of human genital cancer. viral dna has been identified in invasive carcinoma of the uterine cervix and in cell lines derived from cervical carcinomas. these sequences are actively transcribed, and translation products corresponding to the early (e)-region genes have been identified. the most abundant viral protein is the e7 protein, which has been shown to possess transforming activity for both established a ... | 1991 | 1714516 |
| office therapy for human papillomavirus infection in nongenital sites. | the many different treatment possibilities for the eradication of warts provide evidence that no single method that is completely effective has been found. although the various methods described herein are usually successful therapies for warts, they are all associated with treatment failures and side effects. until the perfect cure for warts is discovered, the physician must evaluate every wart carefully before deciding on a course of action. | 1991 | 1711941 |
| a method for detection of human papillomavirus dna in stored slides of stained cervical smears. | a simple method was developed for extraction of dna from stored slides of papanicolaou stained cervical smears. hpv type 16 dna was detected by dna hybridization in 7/10 samples from known hpv-16 positive patients, whereas the 10 samples from negative patients were also negative with our assay. using our method for obtaining data from stained cervical smears, it will be possible to design and perform historical cohort studies on stored material. | 1990 | 1705024 |
| characterization of human antibody-reactive epitopes encoded by human papillomavirus types 16 and 18. | we have previously reported that the most common human serum immunoglobulin g antibody reactivities to human papillomavirus type 16 and type 18 (hpv16 and hpv18)-encoded proteins are directed against the minor capsid proteins (hpv16 l2 and hpv18 l2) and to the e7 protein of hpv16 (s. a. jenison, x.-p. yu, j. m. valentine, l. a. koutsky, a. e. christiansen, a. m. beckmann, and d. a. galloway, j. infect. dis. 162:60-69, 1990). in this study, the antibody-reactive segments of the hpv16 e7, hpv16 l2 ... | 1991 | 1704924 |