Publications
Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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a photopolymerized antimicrobial hydrogel coating derived from epsilon-poly-l-lysine. | hydrogels made from epsilon-poly-l-lysine-graft-methacrylamide (epl-ma) have been found to have impressive wide spectrum antimicrobial activity against both bacteria (specifically escherichia coli, pseudomonas aeruginosa, serratia marcescens and staphylococcus aureus) and fungi (specifically candida albicans and fusarium solani). the epl-ma hydrogel also possesses in vitro biocompatibility and epl-ma solution is relatively non-hemolytic: the concentration needed for onset of human red blood cell ... | 2011 | 21257199 |
in vivo confocal microscopy of equine fungal keratitis. | to describe in vivo corneal confocal microscopy of horses with fungal keratitis and correlate findings with clinical, histopathological, and microbiological evaluations of clinical cases and an ex vivo experimental equine fungal keratitis model. | 2011 | 21199274 |
[fusarium solani infection in a patient after allogeneic hemotopoietic stem cell transplantation: case report and literature review]. | to study fusarium solani infection as a complication in patients after allogeneic hemotopoietic stem cell transplantation and to discuss the diagnosis and appropriate therapy. | 2010 | 21176501 |
antifungal activity of pvd1 defensin involves plasma membrane permeabilization, inhibition of medium acidification, and induction of ros in fungi cells. | in recent years, studies have demonstrated the function of many antimicrobial peptides against an extensive number of microorganisms that have been isolated from different plant species and that have been used as models for the study of various cellular processes linked to these peptides' activities. recently, a new defensin from phaseolus vulgaris (l.) seeds, named pvd(1,) was isolated and characterized. pvd(1) was purified through anion exchange and phase-reverse chromatography. pvd(1)'s antif ... | 2010 | 21170711 |
changing indications for lamellar keratoplasty in shandong, 1993 - 2008. | with the advancement of microsurgical techniques, lamellar keratoplasty (lk) has been more valued and performed to treat corneal blindness. this study aimed to evaluate the indications and changing trends for lk during the past 16 years in shandong eye institute, an eye center in china. | 2010 | 21163128 |
a polycationic antimicrobial and biocompatible hydrogel with microbe membrane suctioning ability. | despite advanced sterilization and aseptic techniques, infections associated with medical implants have not been eradicated. most present coatings cannot simultaneously fulfil the requirements of antibacterial and antifungal activity as well as biocompatibility and reusability. here, we report an antimicrobial hydrogel based on dimethyldecylammonium chitosan (with high quaternization)-graft-poly(ethylene glycol) methacrylate (dmdc-q-g-em) and poly(ethylene glycol) diacrylate, which has excellent ... | 2010 | 21151166 |
sudden-death syndrome of soybean is caused by two morphologically and phylogenetically distinct species within the fusarium solani species complex--f. virguliforme in north america and f. tucumaniae in south america. | soybean sudden-death syndrome has become a serious constraint to commercial production of this crop in north and south america during the past decade. to assess whether the primary etiological agent is panmictic in both hemispheres, morphological and molecular phylogenetic analyses were conducted on strains selected to represent the known pathogenic and genetic diversity of this pathogen. maximum-parsimony analysis of dna sequences from the nuclear ribosomal intergenic spacer region and the sing ... | 2003 | 21148975 |
identification of fusarium species in formalin-fixed and paraffin-embedded sections by in situ hybridization using peptide nucleic acid probes. | fusarium has recently emerged as an opportunistic pathogen of humans, but the histological differentiation of fusarium from aspergillus and scedosporium is particularly difficult because these fungi may induce similar clinical features and exhibit filamentous development in host tissues. thus, there is a need to establish rapid and reliable methods that are applicable to pathological diagnoses. the aim of this study was to evaluate and establish in situ hybridization (ish) using peptide nucleic ... | 2010 | 21106796 |
[expression of integrin during the course of adhesion between fungi and corneal epithelial cells]. | to investigate the expression of integrin during the course of adhesion between fungi and corneal epithelial cells. | 2010 | 21092564 |
elimination of hydrophobic volatile organic compounds in fungal biofilters: reducing start-up time using different carbon sources. | fungal biofilters have been recently studied as an alternative to the bacterial systems for the elimination of hydrophobic volatile organic compounds (voc). fungi foster reduced transport limitation of hydrophobic vocs due to their hydrophobic surface and extended gas exchange area associated to the hyphal growth. nevertheless, one of their principal drawbacks is their slow growth, which is critical in the start-up of fungal biofilters. this work compares the use of different carbon sources (gly ... | 2010 | 21077141 |
enzymes for the biofunctionalization of poly(ethylene terephthalate). | the functionalization of synthetic polymers such as poly(ethylene terephthalate) to improve their hydrophilicity can be achieved biocatalytically using hydrolytic enzymes. a number of cutinases, lipases, and esterases active on polyethylene terephthalate have been identified and characterized. enzymes from fusarium solani, thermomyces insolens, t. lanuginosus, aspergillus oryzae, pseudomonas mendocina, and thermobifida fusca have been studied in detail. thermostable biocatalysts hydrolyzing poly ... | 2010 | 21076908 |
action of supernatants from combined growth of fusarium solani and pseudomonas aeruginosa against the tubercle bacillus. | 1946 | 21026122 | |
transgenically expressed rice germin-like protein1 in tobacco causes hyper-accumulation of h2o2 and reinforcement of the cell wall components. | our recent report documented that the rice germin-like protein1 (osglp1), being a cell wall-associated protein involves in disease resistance in rice and possesses superoxide dismutase (sod) activity as recognized by heterologous expression in tobacco. in the present study, the transgenic tobacco plants were analyzed further to decipher the detailed physiological and biochemical functions of the osglp1 and its associated sod activity. the transgenic tobacco lines expressing sod-active osglp1 sho ... | 2010 | 20971065 |
dermatitis in captive wyoming toads (bufo baxteri) associated with fusarium spp. | from may 2007 to june 2008, 30 of 49 wyoming toads (bufo baxteri) kept at omaha's henry doorly zoo (nebraska, usa) died showing clinical signs of ventral erythema, inappetance, lethargy, and delayed righting reflex. treatment with antifungals and antibiotics was unsuccessful in all cases. histopathologic analyses revealed dermatitis as the primary problem in 20 of 21 toads in which skin was examined. fungal dermatitis was present in 17 toads, with hyphae approximately 1-3 μm in diameter, and par ... | 2010 | 20966269 |
successful treatment of fusarium solani ecthyma gangrenosum in a patient affected by leukocyte adhesion deficiency type 1 with granulocytes transfusions. | ecthyma gangrenosum (eg) manifests as a skin lesion affecting patients suffering extreme neutropenia and is commonly associated with pseudomonas aeruginosa in immunocompromised patients. leukocyte adhesion deficiency i (lad i) which count among primary immunodeficiency syndromes of the innate immunity, is an autosomal recessive disorder characterized in its severe phenotype by a complete defect in cd18 expression on neutrophils, delayed cord separation, chronic skin ulcers mainly due to recurren ... | 2010 | 20929531 |
direct enzymatic acylation of cellulose pretreated in bmimcl ionic liquid. | cellulose esters are an important class of functional biopolymers with great interest in the chemical industry. in this work the enzymatic acylation of avicel cellulose with vinyl propionate, vinyl laurate and vinyl stearate, has been performed successfully in a solvent free reaction system. at first cellulose was putted into the ionic liquid bmimcl (1-n-butyl-3-methylimidazolium chloride) in order to facilitate the unwrap of the structure of the polysaccharide molecule and make it accessible to ... | 2010 | 20888759 |
fusarium solani invader of the eggs of the insectpanstrongylus geniculatus in a vivarium. | a strain offusarium solani sensu snyder & hansen invaded the eggs of the insectpanstrongylus geniculatus in a vivarium. none of the invaded eggs hatched. to establish experimentally the pathogenicity of thisfusarium species against the eggs ofp. geniculatus, the fungus and the eggs were incubated together under different relative humidities and temperatures. at 64% relative humidity and 26 °c, the fungus grew well colonizing and penetrating all of the chorions.three embryos died and were also co ... | 1996 | 20882454 |
fusarium solani is responsible for mass mortalities in nests of loggerhead sea turtle, caretta caretta, in boavista, cape verde. | the fungus fusarium solani (mart.) saccardo (1881) was found to be the cause of infections in the eggs of the sea turtle species caretta caretta in boavista island, cape verde. egg shells with early and severe symptoms of infection, as well as diseased embryos were sampled from infected nests. twenty-five isolates with similar morphological characteristics were obtained. their its rrna gene sequences were similar to the genbank sequences corresponding to f. solani and their maximum identity rang ... | 2010 | 20875054 |
solar disinfection of fungal spores in water aided by low concentrations of hydrogen peroxide. | our previous contribution showed that fusarium solani spores are inactivated by low amounts of hydrogen peroxide (lower than 50 mg l(-1)) together with solar irradiation in bottles. the purpose of the current study was to evaluate the effectiveness of solar h(2)o(2)/uv-vis in distilled water and simulated municipal wastewater treatment plant effluent (se) contaminated with chlamydospores of fusarium equiseti in a 60 l solar cpc photo-reactor under solar irradiation. this study showed that f. equ ... | 2011 | 20859602 |
effect of biofumigation with manure amendments and repeated biosolarization on fusarium densities in pepper crops. | in the region of murcia (southeast spain), sweet pepper has been grown as a monoculture in greenhouses for many years. until 2005, when it was banned, soils were disinfested with methyl bromide (mb) to control pathogens and to prevent soil fatigue effects. the genus fusarium plays an important role in the microbiological component associated with yield decline in pepper monocultures. in the present study, soils were treated with manure amendments, alone (biofumigation, b) or in combination with ... | 2011 | 20820866 |
fusarium keratitis 3 weeks after healed corneal cross-linking. | to report a case of fusarium solani keratitis after corneal cross-linking (cxl) treatment. | 2010 | 20795586 |
a novel class of peptide pheromone precursors in ascomycetous fungi. | recently, sexual development in the heterothallic ascomycete trichoderma reesei (anamorph of hypocrea jecorina) has been achieved and thus initiated attempts to elucidate regulation and determinants of this process. while the α-type pheromone of this fungus fits the consensus known from other fungi, the assumed a-type peptide pheromone precursor shows remarkably unusual characteristics: it comprises three copies of the motif (li)gc(ts)vm thus constituting a caax domain at the c-terminus and two ... | 2010 | 20735770 |
biocidal efficacy of silver-impregnated contact lens storage cases in vitro. | silver-impregnated contact lens (cl) storage cases are designed to reduce microbial contamination during use, but there are limited data on their effectiveness. this study evaluated early antimicrobial activity of silver-impregnated cl cases and silver-release characteristics in vitro. | 2011 | 20720221 |
purification and characterization of an extracellular laccase from the anthracene-degrading fungus fusarium solani mas2. | an extracellular laccase was purified from the culture medium of the non-white rot, anthracene-degrading fungal strain fusarium solani mas2. both native page and sds-page revealed one single band corresponding to a molecular weight of about 72 kda. treatment with endoglycosidase h reduced the molecular weight by 12%. the purified laccase maintained stable at ph 3-11 and up to 50 degrees c. the highest activity was detected at ph 3.0 and at 70 degrees c. the enzyme retained 46.2-97.2% of it activ ... | 2010 | 20716485 |
biodegradation of anthracene and benz[a]anthracene by two fusarium solani strains isolated from mangrove sediments. | an investigation was undertaken on the biodegradation of two kinds of polycyclic aromatic hydrocarbons (pahs), anthracene (ant) and benz[a]anthracene (baa), by fungi isolated from pah-contaminated mangrove sediments environment in ma wan, hong kong. ant (50mg l(-1)) and baa (20mg l(-1)), respectively, were added to mineral salt medium initially for screening of pah-degrading fungi, and finally two fungal species capable of using ant or baa as the sole carbon source were isolated and identified a ... | 2010 | 20691587 |
agar block smear preparation: a novel method of slide preparation for preservation of native fungal structures for microscopic examination and long-term storage. | we describe a novel method of fungal slide preparation named "agar block smear preparation." a total of 510 agar block smears of 25 fungal strains obtained from culture collections, 90 qc fungal strains, and 82 clinical fungal strains from our clinical microbiology laboratory, which included a total of 137 species of yeasts, molds, and thermal dimorphic fungi, were prepared and examined. in contrast to adhesive tape preparation, agar block smears preserved the native fungal structures, such as i ... | 2010 | 20660221 |
antimicrobial activity of simulated solar disinfection against bacterial, fungal, and protozoan pathogens and its enhancement by riboflavin. | riboflavin significantly enhanced the efficacy of simulated solar disinfection (sodis) at 150 watts per square meter (w m(-2)) against a variety of microorganisms, including escherichia coli, fusarium solani, candida albicans, and acanthamoeba polyphaga trophozoites (>3 to 4 log(10) after 2 to 6 h; p < 0.001). with a. polyphaga cysts, the kill (3.5 log(10) after 6 h) was obtained only in the presence of riboflavin and 250 w m(-2) irradiance. | 2010 | 20639371 |
development of a new mlst scheme for differentiation of fusarium solani species complex (fssc) isolates. | fungi belonging to the fusarium solani species complex (fssc) are well known plant pathogens. in addition to being the causative agent of some superficial infections, fssc has recently emerged as a group of common opportunistic moulds, mainly in patients with haematological malignancies. molecular typing methods are essential in order to better understand the epidemiology of such opportunistic agents with the final goal of preventing contamination. a three-locus typing scheme has thus been devel ... | 2010 | 20624428 |
novel short antibacterial and antifungal peptides with low cytotoxicity: efficacy and action mechanisms. | short antimicrobial peptides with nine and eleven residues were developed against several clinically important bacterial and fungal pathogens (specifically escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, candida albicans, and fusarium solani). twelve analogues of previously reported peptides bp76 (kklfkkilkfl) and pac-525 (kwrrwvrwi) were designed, synthesized, and tested for their antimicrobial activities. two of our eleven amino acid peptides, p11-5 (gklfkkilkil) and p11-6 (kk ... | 2010 | 20603106 |
characterization of a 20 kda dnase elicitor from fusarium solani f. sp. phaseoli and its expression at the onset of induced resistance in pisum sativum. | summary dnase released from fusarium solani f. sp. phaseoli (fsph dnase) has previously been reported to induce pathogenesis-related (pr) genes, phytoalexin accumulation and disease resistance against subsequent challenge with the true pea pathogen, fusarium solani f. sp. pisi (fspi). this report is a further analysis of dnase production with probes specific for both the gene and protein. n-terminal analysis of the approximately 20 kda fsph dnase protein facilitated both the development of anti- ... | 2001 | 20573002 |
oxidation of elemental sulfur by fusarium solani strain thif01 harboring endobacterium bradyrhizobium sp. | nineteen fungal strains having an ability to oxidize elemental sulfur in mineral salts medium were isolated from deteriorated sandstones of angkor monuments. these fungi formed clearing zone on agar medium supplemented with powder sulfur due to the dissolution of sulfur. representative of the isolates, strain thif01, was identified as fusarium solani on the basis of morphological characteristics and phylogenetic analyses. pcr amplification targeting 16s rrna gene and analyses of full 16s rrna ge ... | 2010 | 20571793 |
analysis of pea hmg-i/y expression suggests a role in defence gene regulation. | summary hmg-i/y proteins are characterized by the presence of at-hook motifs, dna binding domains that recognize at-rich tracts of dna. by facilitating protein:protein and protein:dna interactions in the vicinity of these at-rich binding sites, hmg-i/y positively or negatively regulates gene expression. several pea defence gene promoters have at-rich tracts of dna that are potential targets for modulation via hmg-i/y. in this study, a comparison of the expression of a pea defence gene (drr206) m ... | 2003 | 20569385 |
fungal outbreak in a show cave. | castañar de ibor cave (spain) was discovered in 1967 and declared a natural monument in 1997. in 2003 the cave was opened to public visits. despite of extensive control, on 26 august 2008 the cave walls and sediments appeared colonized by long, white fungal mycelia. this event was the result of an accidental input of detritus on the afternoon of 24 august 2008. we report here a fungal outbreak initiated by mucor circinelloides and fusarium solani and the methods used to control it. | 2010 | 20553941 |
miniemulsion as efficient system for enzymatic synthesis of acid alkyl esters. | the aim of this work is to devise an efficient enzymatic process for the production of linear alkyl esters in aqueous miniemulsion systems. the esterification reactions of linear alcohols and carboxylic acids were performed with three different enzymes, commercial amano lipase ps from pseudomonas cepacia, lipase type vii from candida rugosa, and lyophilized fusarium solani pisi cutinase expressed in saccharomyces cerevisiae su50. the miniemulsion system shows a high potential for the synthesis o ... | 2010 | 20503297 |
the genomic organization of plant pathogenicity in fusarium species. | comparative genomics is a powerful tool to infer the molecular basis of fungal pathogenicity and its evolution by identifying differences in gene content and genomic organization between fungi with different hosts or modes of infection. through comparative analysis, pathogenicity-related chromosomes have been identified in fusarium oxysporum and fusarium solani that contain genes for host-specific virulence. lateral transfer of pathogenicity chromosomes, inferred from genomic data, now has been ... | 2010 | 20471307 |
do multipurpose contact lens disinfecting solutions work effectively against non-fda/iso recommended strains of bacteria and fungi? | recent outbreaks of microbial keratitis have increased concerns about the efficacy of multipurpose solutions (mps) against 'real-world' organisms. this study determined, in accordance with fda/iso standard methods, the effects of five mps against clinical isolates and type strains of bacteria, and isolates of fungi from subjects' ocular structures; and of three mps against environmental fungal isolates. | 2010 | 20444107 |
direct site-directed photocoupling of proteins onto surfaces coated with beta-cyclodextrins. | a method called dock'n'flash was developed to offer site-specific capture and direct uva-induced photocoupling of recombinant proteins. the method involves the tagging of recombinant proteins with photoreactive p-benzoyl-l-phenylalanine (pbpa) by genetic engineering. the photoreactive pbpa tag is used for affinity capture of the recombinant protein by beta-cyclodextrin (beta-cd), which provides hydrogen atoms to be abstracted in the photocoupling process. to exemplify the method, a recombinant, ... | 2010 | 20441154 |
knock down of chitosanase expression in phytopathogenic fungus fusarium solani and its effect on pathogenicity. | chitosanases are lytic enzymes involved in the degradation of chitosan, a component of fungal cell walls. the phytopathogenic fungus fusarium solani produces an extracellular chitosanase, csn1, the role of which in the physiology and virulence of the fungus remains to be expounded. here, we studied the expression of the csn1 gene through gene silencing and examined its effect on fungal pathogenicity. a vector construct encoding a hairpin rna (hprna) of csn1 was constructed and introduced into th ... | 2010 | 20419375 |
mycotic keratitis in india: a five-year retrospective study. | mycotic keratitis is a fungal infection of the cornea. this infection is difficult to treat and it can lead to severe visual impairment or blindness. it is worldwide in distribution, but is more common in the tropics and subtropical regions. trauma is the major predisposing factor, followed by ocular and systemic defects, prior application of corticosteroids, and prolonged use of antibiotic eye-drops. the objective of this study was to determine causative agents and to identify the predisposing ... | 2010 | 20351459 |
other fungi causing onychomycosis. | nondermatophyte onychomycosis account for 2% to 12% of all nail fungal infections and can be caused by a wide range of fungi, mainly scopulariopsis brevicaulis, aspergillus versicolor, a. flavus, a. niger, a. fumigatus, fusarium solani, f. oxysporum and scytalidium spp. among the predisposing factors are footwear, hyperhidrosis, local trauma, peripheral circulatory disease, and immunosuppression. these nondermatophyte fungi lack the keratinolytic capacity of dermatophytes, but they still can inf ... | 2010 | 20347658 |
[mycotic keratitis in an eye care hospital in mexico city]. | some of the most common precipitating events for keratomycoses (fungal keratitis), include surgical trauma (after cornea transplantation), the use of contaminated contact lenses or alterations in lacrimal secretions. diagnosis and treatment (to avoid loss of vision) for these type of infections are challenging. | 2010 | 20346302 |
metal based biologically active compounds: design, synthesis, and antibacterial/antifungal/cytotoxic properties of triazole-derived schiff bases and their oxovanadium(iv) complexes. | a new series of oxovanadium(iv) complexes have been designed and synthesized with a new class of triazole schiff bases derived from the reaction of 3,5-diamino-1,2,4-triazole with 2-hydroxy-1-naphthaldehyde, pyrrole-2-carboxaldehyde, pyridine-2-carboxaldehyde and acetyl pyridine-2-carboxaldehyde, respectively. physical (magnetic susceptibility, molar conductance), spectral (ir, (1)h nmr, (13)c nmr, mass and electronic) and analytical data have established the structures of these synthesized schi ... | 2010 | 20338672 |
acidified nitrite enhances hydrogen peroxide disinfection of acanthamoeba, bacteria and fungi. | in the human innate immune system, stimulated phagocytes release reactive nitrogen intermediates that can react with superoxide to form the powerful oxidant peroxynitrite and other less abundant species. in this study, the efficacy of hydrogen peroxide (h2o2) and acidified nitrite (nano2) alone and in combination was compared against a variety of bacteria, fungi and protozoa. | 2010 | 20335189 |
on the structure and possible functions of a pigment of fusarium solani d2 purple. | 1947 | 20270776 | |
synthesis and antileishmanial and antimicrobial activities of some 2,3-disubstituted 3h-quinazolin-4-ones. | a series of 2,3-disubstituted 3h-quinazolin-4-ones was synthesized. antimicrobial activities of the synthesized compounds were investigated against gram (+ve) and gram (-ve) bacteria, including b. subtilis, s. aureus, s. flexneri, p. aeruginosa, and s. typhi, and six fungi, namely trichophyton longifusus, candida albicans, aspergillus flavus, microsporum canis, fusarium solani, and candida glabrata using the broth microdilution method. compounds 9, 11, and 12 showed significant activities agains ... | 2010 | 20235747 |
natural occurrence of the fusarium solani on tityus stigmurus (thorell, 1876) (scorpiones: buthidae). | members of the fusarium solani species complex are agents of human mycoses, also affecting plants and other animals. nevertheless, this fungus has not been reported on scorpions. ten specimens of tityus stigmurus collected in the field and showing their surface covered by white mycelia were used to assess fungus presence in the animal after its death. identification of the fungi was based upon the cultural and morphological characteristics. the fungus was isolated from chelicerae and intersegmen ... | 2010 | 20231972 |
[characterization of a chitosanase from fusarium solani and its expression in an industrial strain of saccharomyces cerevisiae]. | to investigate the biochemical characteristics of the chitosanase from fusarium solani, and its application in chitooligosaccharides production, and express the chitosanase gene (csn) in a saccharomyces cerevisiae industrial strain. | 2009 | 20222446 |
endophytic fungi diversity of aquatic/riparian plants and their antifungal activity in vitro. | two hundred and fourteen endophytic fungi were isolated from 500 segments of aquatic/riparian plants ottelia acuminata, myriophyllum verticillatum, equisetum arvense, cardamine multijuga, and impatiens chinensis. they were identified to 31 taxa in which cladosporium, fusarium, and geotrichum were the dominant genera. among all isolates, 169 (79%) were anamorphic fungi, 1 (0.5%) was an teleomorphic ascomycete and 44 (21%) were sterile mycelia. there were significant differences in the colonizatio ... | 2010 | 20221722 |
a case of primary localized cutaneous infection due to fusarium oxysporum. | fusarium is a ubiquitous hyalohyphomycete isolated from food, widespread in the environment (plants, soil) and present at all latitudes. fusarium oxysporum and fusarium solani are the most frequent pathogenic species, followed by f. moniliforme and f. chlamydosporum. infections due to this mold may be disseminated or localized. localized forms include cutaneous and subcutaneous infection, onychomycosis, endophtalmitis, otitis, sinusitis, arthritis, osteomyelitis, and brain abscess. disseminated ... | 2010 | 20177971 |
molecular phylogenetic diversity of dermatologic and other human pathogenic fusarial isolates from hospitals in northern and central italy. | fifty-eight fusaria isolated from 50 italian patients between 2004 and 2007 were subject to multilocus dna sequence typing to characterize the spectrum of species and circulating sequence types (sts) associated with dermatological infections, especially onychomycoses and paronychia, and other fusarioses in northern and central italy. sequence typing revealed that the isolates were nearly evenly divided among the fusarium solani species complex (fssc; n = 18), the f. oxysporum species complex (fo ... | 2010 | 20107100 |
two cutinase-like proteins secreted by mycobacterium tuberculosis show very different lipolytic activities reflecting their physiological function. | cutinases are extracellular enzymes that are able to degrade cutin, a polyester protecting plant leaves and many kinds of lipids. although cutinases are mainly found in phytopathogenic fungi or bacteria, 7 genes related to the cutinase family have been predicted in the genome of mycobacterium tuberculosis. these genes may encode proteins that are involved in the complex lipid metabolism of the bacterium. here, we report on the biochemical characterization of two secreted proteins of m. tuberculo ... | 2010 | 20103719 |
improvement of sec-dependent secretion of a heterologous model protein in bacillus subtilis by saturation mutagenesis of the n-domain of the amye signal peptide. | due to the lack of an outer membrane, gram-positive bacteria (e.g., bacillus species) are considered as promising host organisms for the secretory production of biotechnologically relevant heterologous proteins. however, the yields of the desired target proteins were often reported to be disappointingly low. here, we used saturation mutagenesis of the positively charged n-domain (positions 2-7) of the signal peptide of the bacillus subtilis alpha-amylase (amye) as a novel approach for the improv ... | 2010 | 20077115 |
[culture-filtrate producing condition and biological activity of fusarium solani]. | to study the culture-filtrate producing condition of fusarium solani isolated from astragalus root and explore the mechanism astragalus root rot disease caused by, in order to find theoretical support for screening resistant germ plasma via mycotoxin. | 2009 | 20069894 |
efficacy of posaconazole as treatment and prophylaxis against fusarium solani. | invasive fusariosis is a highly aggressive fungal infection associated with high mortality in heavily immunocompromised patients. although posaconazole is efficacious as salvage therapy against infections caused by fusarium species, concerns remain regarding this agent in the setting of reduced potency. to evaluate the efficacy of posaconazole as treatment or prophylaxis against invasive fusariosis caused by fusarium solani, we utilized a neutropenic murine model of disseminated disease. icr mic ... | 2010 | 20065054 |
multi-enzyme inhibition assay for the detection of insecticidal organophosphates and carbamates by high-performance thin-layer chromatography applied to determine enzyme inhibition factors and residues in juice and water samples. | esterase inhibition assays provide an effect-directed tool of rapid screening for inhibitors in environmental and food samples. according to a multi-enzyme microtiter-plate assay, rabbit liver esterase (rle), bacillus subtilis esterase (bs2), and cutinase from fusarium solani pisi (cut) were used for the detection of 21 organophosphorus and carbamate pesticides by high-performance thin-layer chromatography-enzyme inhibition assays (hptlc-ei). staining was performed with fast blue salt b coupling ... | 2010 | 20060788 |
the microbiology of lascaux cave. | lascaux cave (montignac, france) contains paintings from the upper paleolithic period. shortly after its discovery in 1940, the cave was seriously disturbed by major destructive interventions. in 1963, the cave was closed due to algal growth on the walls. in 2001, the ceiling, walls and sediments were colonized by the fungus fusarium solani. later, black stains, probably of fungal origin, appeared on the walls. biocide treatments, including quaternary ammonium derivatives, were extensively appli ... | 2010 | 20056706 |
onychomycosis caused by fusarium solani in a woman with diabetes. | a case of untreated fusarial onychomycosis leading to serious consequences is reported. fusarium solani is a widespread fungus and an occasional human pathogen. it usually invades rapidly in immunocompromised hosts, and often results in a poor outcome despite treatment. we report a woman with diabetes mellitus who had untreated fusarial infection of the nails, which developed into subcutaneous fusariosis, superinfected by bacteria, and then evolved into osteomyelitis that subsequently resulted i ... | 2009 | 20055843 |
a dark strain in the fusarium solani species complex isolated from primary subcutaneous sporotrichioid lesions associated with traumatic inoculation via a rose bush thorn. | fusarium species are hyaline hyphomycetes widely distributed in nature and documented agents of both superficial and systemic infections in humans. in this paper, we report a darkly-pigmented and initially non-sporulating isolate in the fusarium solani species complex (fssc) causing a post-traumatic sporotrichoid infection in an otherwise healthy, male patient. sequencing of multiple loci showed that the isolate represented an otherwise unknown lineage, possibly corresponding to a separate speci ... | 2010 | 20055744 |
concurrent acanthamoeba and fusarium keratitis with silicone hydrogel contact lens use. | purpose:: to report a case of simultaneous acanthamoeba and fusarium keratitis associated with no-rob multipurpose contact lens solution and silicone hydrogel contact lens use. method:: observational case report results:: a 39-ycar-old woman was referred for worsening of a presumed bacterial conical ulcer in the setting of silicone hydrogel lens wear with occasional overnight wear, no-rub multipurpose contact lens solution use, and combined topical antibiotic/corticosteroid treatment. initial co ... | 2009 | 20023585 |
silver enhances the in vitro antifungal activity of the saponin, cay-1. | summary the fungicidal properties of purified cay-1, dissolved silver ion and ethylenediamine tetraacetic acid (edta) separately were studied in vitro as were the abilities of silver and edta to enhance cay-1 fungicidal properties. non-germinated and germinating conidia of aspergillus flavus, aspergillus fumigatus, aspergillus niger, fusarium verticillioides (fusarium moniliforme), fusarium oxysporum and fusarium solani were incubated separately with cay-1 (0-24.8 mug ml(-1)), silver (0-111.1 mu ... | 2009 | 20002309 |
identifying sequences potentially related to resistance response of piper tuberculatum to fusarium solani f. sp. piperis by suppression subtractive hybridization. | piper tuberculatum is an exotic piper from the amazon region that shows resistance to infection by fusarium solani f. sp. piperis, causal agent of fusarium disease in black pepper (piper nigrum l.). in this work we aimed to study the interaction between p. tuberculatum and f. solani f. sp. piperis at a molecular level, using suppression subtractive hybridization to identify genes potentially related to fusarium disease resistance. comparative sequence analysis confirmed that clones isolated here ... | 2009 | 20001904 |
an outbreak of early-onset endophthalmitis caused by fusarium species following cataract surgery. | this study aimed to report an outbreak of early-onset endophthalmitis caused by fusarium species following cataract surgery. | 2009 | 19958115 |
transesterification of oil mixtures catalyzed by microencapsulated cutinase in reversed micelles. | recombinant cutinase from fusarium solani pisi was used to catalyze the transesterification reaction between a mixture of triglycerides (oils) and methanol in reversed micelles of bis(2-ethylhexyl) sodium sulfosuccinate (aot) in isooctane for the purposes of producing biodiesel. the use of a bi-phase lipase-catalyzed system brings advantages in terms of catalyst re-use and the control of water activity in the medium and around the enzyme micro-environment. small-scale batch studies were performe ... | 2010 | 19943181 |
[successful treatment of resistant fusarium solani keratitis with liposomal amphotericin b]. | the prognosis for fusarium keratitis is poor. effective drugs to treat this infection are therefore needed. | 2009 | 19942316 |
pyrene degradation and copper and zinc uptake by fusarium solani and hypocrea lixii isolated from petrol station soil. | this study aimed to isolate and identify potential polycyclic aromatic hydrocarbon (pah)-degrading and/or metal-tolerant fungi from pah-contaminated and metal-contaminated soils. | 2010 | 19922595 |
biodegradation of low-density polyethylene (ldpe) by isolated fungi in solid waste medium. | in this study, biodegradation of low-density polyethylene (ldpe) by isolated landfill-source fungi was evaluated in a controlled solid waste medium. the fungi, including aspergillus fumigatus, aspergillus terreus and fusarium solani, were isolated from samples taken from an aerobic aged municipal landfill in tehran. these fungi could degrade ldpe via the formation of a biofilm in a submerged medium. in the sterilized solid waste medium, lpde films were buried for 100 days in a 1-l flask containi ... | 2010 | 19919893 |
a murine model of contact lens-associated fusarium keratitis. | fusarium solani and f. oxysporum were the causative organisms of the 2005/2006 outbreak of contact lens-associated fungal keratitis in the united states. the present study was an investigation of the ability of f. oxysporum grown as a biofilm on silicone hydrogel contact lenses to induce keratitis. | 2010 | 19875664 |
endophytic fungal strains of fusarium solani, from apodytes dimidiata e. mey. ex arn (icacinaceae) produce camptothecin, 10-hydroxycamptothecin and 9-methoxycamptothecin. | camptothecin and 10-hydroxycamptothecin are two important precursors for the synthesis of the clinically useful anticancer drugs, topotecan and irinotecan. in recent years, efforts have been made to identify novel plant and endophytic fungal sources of camptothecin and 10-hydroxycamptothecin. in this study we have isolated endophytic fungi strains from apodytes dimidiata (icacinaceae), a medium sized tree from the western ghats, india. the fungi were identified as fusarium solani using both its ... | 2010 | 19863979 |
parthenium management by cultural filtrates of phytopathogenic fungi. | in the present study, herbicidal activity of culture filtrates of nine phytopathogenic fungi, namely, alternaria alternata (fr.) keissl., drechslera australiensis (bugnicourt) subramanian & jain, drechslera hawaiiensis (curtis and cooke) shoemaker, drechslera biseptata (saccardo & roumeguere) richardson & fraser., drechslera rostrata (drechsler) ricardson & fraser, fusarium oxysporum (massey) synd. & hans., fusarium solani (martius) saccardo., monilia stophila (montagne) and cladosporium sp. (gr ... | 2009 | 19844827 |
endophytic bacteria from ocimum sanctum and their yield enhancing capabilities. | endophytes are beneficial microbes that reside intercellularly inside the plants. interaction of endophytes with the host plants and their function within their host are important to address ecological relevance of endophyte. four endophytic bacteria os-9, os-10, os-11, and os-12 were isolated from healthy leaves of ocimum sanctum. these isolated microbes were screened in dual culture against various phytopathogenic fungi viz. rhizoctonia solani, sclerotium rolfsii, fusarium solani, alternaria s ... | 2010 | 19826860 |
steady state and time resolved fluorescence quenching and chemical modification studies of a lectin from endophytic fungus fusarium solani. | the solute quenching studies of a lectin from endophytic fungus fusarium solani were carried out using different quenchers such as acrylamide, succinimide, potassium iodide and cesium chloride. the lectin showed emission maximum at 348 nm indicating relative exposure of tryptophan. the quenchable fraction of the fluorophore was 100% with acrylamide, whereas it was only 50% with succinimide. the ionic quenchers iodide and cesium showed opposite effects at different ph. in the case of cesium, rais ... | 2010 | 19823920 |
structural and functional studies of aspergillus oryzae cutinase: enhanced thermostability and hydrolytic activity of synthetic ester and polyester degradation. | cutinases are responsible for hydrolysis of the protective cutin lipid polyester matrix in plants and thus have been exploited for hydrolysis of small molecule esters and polyesters. here we explore the reactivity, stability, and structure of aspergillus oryzae cutinase and compare it to the well-studied enzyme from fusarium solani. two critical differences are highlighted in the crystallographic analysis of the a. oryzae structure: (i) an additional disulfide bond and (ii) a topologically favor ... | 2009 | 19810726 |
antifungal mechanism of a novel antifungal protein from pumpkin rinds against various fungal pathogens. | a novel antifungal protein (pr-2) was identified from pumpkin rinds using water-soluble extraction, ultrafiltration, cation exchange chromatography, and reverse-phase high-performance liquid chromatography. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry indicated that the protein had a molecular mass of 14865.57 da. automated edman degradation showed that the n-terminal sequence of pr-2 was qgigvgdndgkrgkr-. the pr-2 protein strongly inhibited in vitro growth of bot ... | 2009 | 19807165 |
genetic diversity in the fungus fusarium solani f.sp. cucurbitae race 1, the casual agent of root and crown rot of cucurbits in iran, using molecular markers. | fusarium solani f.sp. cucurbitae race 1 is a pathogen on cucurbit plants. in this study genetic diversity among 26 isolates of fusarium solani f.sp. cucurbitae race 1 was studied using restriction fragment length polymorphism (rflp) of its (interal transcribed spacer) regions and random amplified polymorphic dnas (rapd) markers. outcome of digestion with six restriction enzymes including ecor i, rsa i, bme 181, msp i, hae iii and hind iii, together with the patterns of restriction fragment lengt ... | 2009 | 19803117 |
design of eudragit rl 100 nanoparticles by nanoprecipitation method for ocular drug delivery. | the objective of the current study was to prepare positively charged amphotericin-b-loaded nanoparticles providing a controlled release formulation. the particles were prepared by solvent displacement or nanoprecipitation method. the non-biodegradable positively charged polymer eudragit rl 100 was used to prepare the different formulations with varying ratios of drug and polymer. the formulations were evaluated in terms of particle size, zeta potential, and differential scanning calorimetry meas ... | 2010 | 19800990 |
the role of short-range cys171-cys178 disulfide bond in maintaining cutinase active site integrity: a molecular dynamics simulation. | understanding structural determinants in enzyme active site integrity can provide a good knowledge to design efficient novel catalytic machineries. fusarium solani pisi cutinase with classic triad ser-his-asp is a promising enzyme to scrutinize these structural determinants. we performed two md simulations: one, with the native structure, and the other with the broken cys171-cys178 disulfide bond. this disulfide bond stabilizes a turn in active site on which catalytic asp175 is located. function ... | 2009 | 19781526 |
pr-1, a novel antifungal protein from pumpkin rinds. | a novel antifungal protein, m(r) = ca. 40 kda, was isolated from pumpkin rind and designated pr-1. when purified by anion exchange chromatography and hplc, it inhibited growth of several fungi including botrytis cinerea, fusarium oxysporum, fusarium solani and rhizoctonia solani, as well as the yeast, candida albicans, at 10-20 microm. it did not inhibit growth of escherichia coli or staphylococcus aureus even at 200 microm. laser scanning microscopy of fungal cells exposed to rhodamine-labeled ... | 2010 | 19760117 |
fusarium solani onychomycosis of the thumbnail coinfected with pseudomonas aeruginosa: report of two cases. | fusarium species are non-dermatophytic moulds, which are commonly known soil saprophytes and important plant pathogens, and have been frequently reported to be aetiological agents of opportunistic infections in humans. the prevalence of onychomycosis caused by fusarium species varies in the literature because of geographical differences in mould distribution and diagnostic methods. onychomycosis caused by fusarium species is considered rare in korea, and only four cases have been described to da ... | 2011 | 19751392 |
identification of two gh18 chitinase family genes and their use as targets for detection of the crayfish-plague oomycete aphanomyces astaci. | the oomycete aphanomyces astaci is regarded as the causative agent of crayfish plague and represents an evident hazard for european crayfish species. native crayfish populations infected with this pathogen suffer up to 100% mortality. the existence of multiple transmission paths necessitates the development of a reliable, robust and efficient test to detect the pathogen. currently, a. astaci is diagnosed by a pcr-based assay that suffers from cross-reactivity to other species. we developed an al ... | 2009 | 19719847 |
isotope fractionations in the biosynthesis of cell components by different fungi: a basis for environmental carbon flux studies. | abstract the isotope fractionation of carbon from substrates possessing different isotope ratios into fatty acids of polar lipids and amino acids was determined for four different fungi (rhizopus arrhizus, mortierella isabellina, fusarium solani, aspergillus niger). carbon isotope ratios of fungi closely followed that of the substrates. palmitic acid (c16:0), derived from phospholipids, did not display a large carbon isotope fractionation against the substrate. stearic acid (c18:0), however, was ... | 2003 | 19719589 |
the genome of nectria haematococca: contribution of supernumerary chromosomes to gene expansion. | the ascomycetous fungus nectria haematococca, (asexual name fusarium solani), is a member of a group of >50 species known as the "fusarium solani species complex". members of this complex have diverse biological properties including the ability to cause disease on >100 genera of plants and opportunistic infections in humans. the current research analyzed the most extensively studied member of this complex, n. haematococca mating population vi (mpvi). several genes controlling the ability of indi ... | 2009 | 19714214 |
in the aftermath of the fusarium keratitis outbreak: what have we learned? | the 2005-2006 outbreak of fusarium keratitis associated with soft hydrophilic contact lens wear was both unprecedented and unexpected. more than 250 cases have been reported worldwide that have primarily been associated with bausch & lomb renu with moistureloc and, more recently, with bausch and lomb renu multiplus multipurpose contact lens disinfecting solutions. this article documents the outbreak, presenting the time line and the historical and scientific basis for its occurrence. underlying ... | 2007 | 19668512 |
invasive fusariosis with prolonged fungemia in a patient with acute lymphoblastic leukemia: case report and review of the literature. | fusariumspp are rare but important opportunistic pathogens in immunocompromised patients. disseminated fusarial infections occur mostly in patients with hematologic malignancies with myelosuppressive chemotherapy or in patients with severe immunodeficiency. although more frequent than aspergillus fungemia, fusarium fungemia remains a rare event. we describe the case of a female patient with febrile neutropenia and persistent fungemia due to fusarium solani, treated with posaconazole and liposoma ... | 2010 | 19665409 |
methylcitrate cycle activation during adaptation of fusarium solani and fusarium verticillioides to propionyl-coa-generating carbon sources. | propionyl-coa is an inhibitor of both primary and secondary metabolism in aspergillus species and a functional methylcitrate cycle is essential for the efficient removal of this potentially toxic metabolite. although the genomes of most sequenced fungal species appear to contain genes coding for enzymes of the methylcitrate cycle, experimental confirmation of pathway activity in filamentous fungi has only been provided for aspergillus nidulans and aspergillus fumigatus. in this study we demonstr ... | 2009 | 19661181 |
hyphal penetration of worn hydrogel contact lenses by fusarium. | to determine the relative susceptibility of worn and unworn hydrogel contact lenses to penetration by hyphae of the fusarium solani-fusarium oxysporum species complex. | 2009 | 19654522 |
improved microwave-mediated synthesis of 3-(3-aryl-1,2,4-oxadiazol-5-yl)propionic acids and their larvicidal and fungal growth inhibitory properties. | the synthesis of 3-(3-aryl-1,2,4-oxadiazol-5-yl)propionic acids from arylamidoximes and succinic anhydride under focused microwave irradiation conditions is described. the new synthetic method furnished the desired products in 2-3 min and good yields. furthermore, the previously complicated purification procedure has been simplified in a manner which is quick, eco-friendly and cost-effective. larvicidal bioassay and fungal growth inhibitory tests were performed using several 3-(3-aryl-1,2,4-oxad ... | 2009 | 19652406 |
fungal biomass is a key factor affecting polymorphonuclear leucocyte-induced hyphal damage of filamentous fungi. | previous studies have not systematically assessed the effect of fungal biomass on polymorphonuclear leucocyte (pmn)-induced hyphal damage (hd) of filamentous fungi. we hypothesised that fungal biomass is a significant factor affecting pmn-induced hd. one isolate each consisting of a volume of 2 x 10(4) conidia ml(-1) of aspergillus fumigatus, aspergillus flavus, aspergillus terreus, rhizopus oryzae, rhizopus microsporus, cunninghamella bertholletiae, scedosporium prolificans and fusarium solani ... | 2010 | 19638003 |
[tumour occurrence of minnow phoxinys phoxinus (l.) from the north dvina and pechora river basins]. | tumours occurrence of minnow and tumour parameters are found to be greatest at the end of june--the first part of july. black tumours give metastases, while gray tumours are separated from the neighboring tissue by a capsule. the stroma of the tumour is pierced through by fungal hyphae of fusarium solani (mart.) app. et wr. the minnow living the first year (the end of it) and the second year (the beginning of it) suffers tumours more frequently. the minnow taken from the saitanovka river estuary ... | 2009 | 19637775 |
backbone dynamics of the antifungal psd1 pea defensin and its correlation with membrane interaction by nmr spectroscopy. | plant defensins are cysteine-rich cationic peptides, components of the innate immune system. the antifungal sensitivity of certain exemplars was correlated to the level of complex glycosphingolipids in the membrane of fungi strains. psd1 is a 46 amino acid residue defensin isolated from pea seeds which exhibit antifungal activity. its structure is characterized by the so-called cysteine-stabilized alpha/beta motif linked by three loops as determined by two-dimensional nmr. in the present work we ... | 2010 | 19632194 |
quantitative determination of gibberellins by high performance liquid chromatography from various gibberellins producing fusarium strains. | high performance liquid chromatographic (hplc) method was developed for analysis of seven gibberellins, i.e., ga3, ga4, ga7, ga3 methyl ester, ga7 methyl ester 3,13 diacetate, ga7 methyl ester, and fusaric acid, using an isocratic system. method was used for estimation of gibberellins from different fusarium strains. gibberellins were extracted from 28 strains of fusarium, out of which six strains of fusarium were isolated from soil of different parts of india and 22 strains were procured from t ... | 2010 | 19626451 |
enzymatic surface hydrolysis of poly(ethylene terephthalate) and bis(benzoyloxyethyl) terephthalate by lipase and cutinase in the presence of surface active molecules. | a lipase from thermomyces lanuginosus and cutinases from thermobifida fusca and fusarium solani hydrolysed poly(ethylene terephthalate) (pet) fabrics and films and bis(benzoyloxyethyl) terephthalate (3pet) endo-wise as shown by maldi-tof-ms, lc-uvd/ms, cationic dyeing and xps analysis. due to interfacial activation of the lipase in the presence of triton x-100, a seven-fold increase of hydrolysis products released from 3pet was measured. in the presence of the plasticizer n,n-diethyl-2-phenylace ... | 2009 | 19616594 |
high-level expression and characterization of fusarium solani cutinase in pichia pastoris. | high-level extracellular production of fusarium solani cutinase was achieved using a pichia pastoris expression system. the cutinase-encoding gene was cloned into ppiczalphaa with the saccharomyces cerevisiae alpha-factor signal sequence and methanol-inducible alcohol oxidase promoter by two different ways. the additional sequences of the c-myc epitope and (his)6-tag of the vector were fused to the c-terminus of cutinase, while the other expression vector was constructed without any additional s ... | 2009 | 19580870 |
flash photolysis of cutinase: identification and decay kinetics of transient intermediates formed upon uv excitation of aromatic residues. | aromatic amino acids play an important role in ultraviolet (uv)-induced photochemical reactions in proteins. in this work, we aim at gaining insight into the photochemical reactions induced by near-uv light excitation of aromatic residues that lead to breakage of disulfide bridges in our model enzyme, fusarium solani pisi cutinase, a lipolytic enzyme. with this purpose, we acquired transient absorption data of cutinase, with supplemental experimental data on tryptophan (trp) and lysozyme as refe ... | 2009 | 19580759 |
induce systemic resistance in lupine against root rot diseases. | root rot caused by soil borne pathogenic fungi is the most sever disease attacks lupine plants. isolation trials from diseased plants in some areas of dakahlia province (egypt) was carried out. rhizoctonia solani and fusarium solani proved to be the most dominant isolates. meanwhile, fusarium oxysporum and sclerotium rolfsii were less frequent. efficacies of some plant resistance elicitors viz.: chitosan (chi), salicylic acid (sa) and hydroquinone (hq) in comparing to the fungicide rhizolex t-50 ... | 2009 | 19579949 |
antifungal activity of nettle (urtica dioica l.), colocynth (citrullus colocynthis l. schrad), oleander (nerium oleander l.) and konar (ziziphus spina-christi l.) extracts on plants pathogenic fungi. | anti-mycotic activity of the ethanol extracts from nettle (urtica dioica l.), colocynth (citrullus colocynthis l. schrad), konar (ziziphus spina-christi l.) and oleander (nerium oleander l.) floral parts were screened in vitro against four important plant pathogenic fungi viz.; alternaria alternate, fusarium oxysporum, fusarium solani and rizoctonia solani using agar dilution bioassay. extracts showed antifungal activity against all the tested fungi. among the plants, nettle and colocynth were t ... | 2009 | 19579919 |
[preliminary study on the use of dna suspension array for the identification of corneal pathogenic fungi]. | to develop a multiplex, microsphere-based dna suspension array for the identification of important pathogenic fungi of cornea and to study the feasibility of its application in the clinical diagnosis of fungal keratitis. | 2009 | 19573328 |
molecular detection of dermatophytes and nondermatophytes in onychomycosis by nested polymerase chain reaction based on 28s ribosomal rna gene sequences. | onychomycosis is often caused by dermatophytes, but the role of nondermatophytes is underestimated due to the difficulty of identifying them by conventional direct microscopy and culture. | 2009 | 19566663 |
continuous hydrolysis of 4-cyanopyridine by nitrilases from fusarium solani o1 and aspergillus niger k10. | the operational stabilities of nitrilases from aspergillus niger k10 and fusarium solani o1 were examined with 4-cyanopyridine as the substrate in continuous-stirred membrane reactors (csmrs). the former enzyme was fairly stable at 30 degrees c with a deactivation constant (k (d)) and enzyme half-life of 0.014 h(-1) and 50 h, respectively, but the latter exhibited an even higher stability characterized by k (d) = 0.008 h(-1) and half-life of 87 h at 40 degrees c. another advantage of this enzyme ... | 2009 | 19554325 |
crystal structure and enhanced activity of a cutinase-like enzyme from cryptococcus sp. strain s-2. | the structural and enzymatic characteristics of a cutinase-like enzyme (cle) from cryptococcus sp. strain s-2, which exhibits remote homology to a lipolytic enzyme and a cutinase from the fungus fusarium solani (fs cutinase), were compared to investigate the unique substrate specificity of cle. the crystal structure of cle was solved to a 1.05 a resolution. moreover, hydrolysis assays demonstrated the broad specificity of cle for short and long-chain substrates, as well as the preferred specific ... | 2009 | 19544571 |
fusarium keratitis in mexico. | to describe the clinical characteristics, diagnosis, treatment, and visual outcome of 61 cases of fusarium keratitis in mexico. | 2009 | 19512910 |