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cytotoxicity and antibacterial activity of gold-supported cerium oxide nanoparticles.cerium oxide nanoparticles (ceo2) have been shown to be a novel therapeutic in many biomedical applications. gold (au) nanoparticles have also attracted widespread interest due to their chemical stability and unique optical properties. thus, decorating au on ceo2 nanoparticles would have potential for exploitation in the biomedical field.201425473288
effect of dietary supplementation of bacteriophage on performance, egg quality and caecal bacterial populations in laying hens.1. bacteriophages (bp) have gained increasing attention as a treatment of bacterial infection for animals. however, the data pertaining to dietary application of bp for laying hens have been limited. 2. this study aimed to investigate the effect of dietary bp on laying performance, egg quality and caecal bacterial populations in laying hens. 3. the dietary bp used in this experiment was a mixture of individual bp targeting salmonella gallinarum, salmonella pullorum, salmonella typhimurium, salmo ...201525471539
dam methylation is required for efficient biofilm production in salmonella enterica serovar enteritidis.the ecological success of salmonella enterica to survive in different environments is due, in part, to the ability to form biofilms, something which is especially important for food industry. the aim of the current study was to evaluate the involvement of dam methylation in biofilm production in s. enteritidis strains. the ability to generate biofilms was analyzed in wild type and dam mutant strains. in s. enteritidis, the absence of dam affected the capacity to develop pellicles at the air-liqu ...201525462918
synthesis, antibacterial, antioxidant activity and qsar studies of novel 2-arylidenehydrazinyl-4-arylthiazole analogues.a novel series of 2-arylidenehydrazinyl-4-arylthiazole analogues (3a-p) was designed and synthesized in excellent yields using a rapid, simple, efficient methodology. sixteen novel compounds were screened for in vitro antimicrobial activities against eleven bacteria, namely, staphylococcus aureus, listeria monocytogenes, enterococcus faecalis, bacillus subtilis, klebsiella pneumonia, citrobacter freundii, cronobacter sakazakii, salmonella enteritidis, escherichia coli, yersinia pestis, and pseud ...201425450634
programming of formalin-induced nociception by neonatal lps exposure: maintenance by peripheral and central neuroimmune activity.the immune and nociceptive systems are shaped during the neonatal period where they undergo fine-tuning and maturation. painful experiences during this sensitive period of development are known to produce long-lasting effects on the immune and nociceptive responses. it is less clear, however, whether inflammatory pain responses are primed by neonatal exposure to mild immunological stimuli, such as with lipopolysaccharide (lps). here, we examine the impact of neonatal lps exposure on inflammatory ...201525449583
[purulent pericarditis and colonic infiltrating to salmonella enteritidis complicated by acute intussusception in a case of il-12rβ1 deficiency].il-12 receptor β1 deficiency (il-12rβ1) predisposes patients to mycobacteria and salmonella infections. we report a case of il-12rβ1 deficiency with a fatal multi-resistant salmonella enteritidis infection. this boy was born after from a consanguineous marriage, and diagnosed as having a il-12rβ1 deficiency since the age of 3 months. he presented with recurrent salmonella enteritidis essentially digestive localization, complicated by purulent pericarditis at the same germ at the age of two and a ...201425445126
prosthetic joint infection due to salmonella species: a case series.prosthetic joint infection (pji) due to salmonella is rare. numerous outbreaks of salmonella have been reported throughout the united states in the last decade. we reviewed and analyzed cases of salmonella pji seen at our institution.201425424009
immunoprotectivity of salmonella enterica serovar enteritidis virulence protein, invh, against salmonella typhi.typhoid fever is a dreadful disease of a major threat to public health in developing countries. vaccination with bacterial immunodominant components such as surface proteins may prove as a potent alternative to live attenuated vaccines. invh, an important part of needle complex in type three secretion system (ttss) plays important role in efficient bacterial adherence and entry into epithelial cells.201425422747
proteomic analysis of outer membrane proteins in salmonella enterica enteritidis.salmonella enterica serovar enteritidis is the predominant agent causing salmonellosis in chickens and other domestic animals. in an attempt to identify antigenic s. enteritidis outer membrane proteins (omps) that may be useful for subunit vaccine development, we established a proteomic map and database of antigenic s. enteritidis omps. in total, 351 and 301 spots respectively from s. enteritidis strain 270 and strain 350 were detected by twodimensional gel electrophoresis. fifty-one antigen-rea ...201525418479
a new series of antibacterial nitrosopyrimidines: synthesis and structure-activity relationship.new nitrosopyrimidines were synthesized and evaluated as potential antibacterial agents. different compounds structurally related with 4,6-bis(alkyl or arylamino)-5-nitrosopyrimidines were evaluated. some of these nitrosopyrimidines displayed significant antibacterial activity against human pathogenic bacteria. among them compounds 1c, 2a-c, and 9a-c exhibited remarkable activity against methicillin-sensitive and -resistant staphylococcus aureus, escherichia coli, yersinia enterocolitica, and sa ...201525413501
potential use of caprylic acid in broiler chickens: effect on salmonella enteritidis.the effect of dietary caprylic acid (ca) on salmonella enteritidis, as well as the surface treatment of chicken skin contaminated with salmonella enteritidis was evaluated. to evaluate the dietary effect of ca on salmonella enteritidis, the individually housed broiler chickens (n=48) were divided into 4 groups (positive control, negative control, 2.5 g/kg of ca in the feed, and 5 g/kg of ca in the feed). the feed of all groups, except the negative control, was artificially contaminated with salm ...201525407657
reduction of salmonella on turkey breast cutlets by plant-derived compounds.the foodborne illnesses associated with poultry meat due to salmonella are a major concern in the united states. in this study, the antimicrobial efficacy of carvacrol, eugenol, thyme essential oil, and trans-cinnamaldehyde was determined against different salmonella serotypes in vitro and on turkey breast cutlets. minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) of antimicrobial agents were determined using a microdilution colorimetric assay. carvacrol was the ...201425405806
salmonella enterica serovar enteritidis enterocolitis during late stages of gestation induces an adverse pregnancy outcome in the murine model.foodborne diseases caused by salmonella enterica serovar enteritidis (s. enteritidis) are a significant health problem. pregnancy, state of immunological tolerance, is a predisposing condition for the development of infections with intracellular pathogens. salmonella species can cause pregnancy complications such as chorioamnionitis, transplacental fetal infection, pre term labor, abortions, neonatal and maternal septicemia. however, the specific mechanisms by which salmonella infections trigger ...201425365504
identification of a napin-like peptide from eugenia malaccensis l. seeds with inhibitory activity toward staphylococcus aureus and salmonella enteritidis.this study aimed to purify and characterize peptides from the seeds of eugenia malaccensis, l. (jambo) with inhibitory activity against the foodborne pathogens staphylococcus aureus and salmonella enteritidis. crude extract (ce), precipitate fraction 30-60 % and molecules between 3.5 and 10 kda obtained from precipitate fraction 30-60 % (em2) showed inhibitory activity against the tested bacterial strains. the highest antibacterial activity was observed for em2 against s. aureus. the major peak ...201425362256
complete genome sequence of salmonella enterica subsp. enterica serovar enteritidis strain sej.salmonella enterica constitutes a group of enteric pathogens with a broad host range, including humans, reptiles, and birds. s. enterica subsp. enterica is a common cause of inflammatory diarrhea in humans. we present the draft genome of s. enterica subsp. enterica serovar enteritidis strain sej, including a 59-kbp plasmid.201425342692
salmonella enterica serovar enteritidis and listeria monocytogenes in mango (mangifera indica l.) pulp: growth, survival and cross-contamination.this study examined the ability of salmonella enterica serovar enteritidis and listeria monocytogenes to grow or survive in mango pulp stored at -20°c, 4°c, 10°c and 25°c, as well as to cross-contaminate mangoes by means of a knife contaminated with different levels of these pathogens.201425328926
tracing the sources of human salmonellosis: a multi-model comparison of phenotyping and genotyping methods.salmonella source attribution is usually performed using frequency-matched models, such as the (modified) dutch and hald models, based on phenotyping data, i.e. serotyping, phage typing, and antimicrobial resistance profiling. however, for practical and economic reasons, genotyping methods such as multi-locus variable number of tandem repeats analysis (mlva) are gradually replacing traditional phenotyping of salmonellas beyond the serovar level. as mlva-based source attribution of human salmonel ...201425315490
prevalence and characterization of salmonella shed by captive and free-range california sea lions (zalophus californianus) from a rehabilitation center and three state reserves along the california coast.salmonella is a genus of zoonotic bacteria that can infect a variety of animals, and may cause gastrointestinal disease in marine mammals. many of the same salmonella serotypes are shed by california sea lions (zalophus californianus) and humans, which poses transmission questions and public health concerns. in this study, 454 fecal samples from three free-ranging california sea lion populations along the california coast and from animals undergoing rehabilitation at the marine mammal center, sa ...201425314819
pyogenic liver abscess caused by salmonella enteritidis: a rare case report.salmonella enteritidis is one of the most important serovars transmitted from animals to humans and a serovar most commonly reported worldwide. infection with enteritidis is mainly limited to the intestinal tract, but under certain circumstances may cross the mucosal barrier to disseminate and get established as some localized infectious focus. although cited as one of the very uncommon causes, enteritidis may involve the liver and evolve into an overt abscess. pyogenic liver abscess by a gas fo ...201425308026
horizontal transmission of salmonella enteritidis in experimentally infected laying hens housed in conventional or enriched cages.the majority of human illnesses caused by salmonella enteritidis are attributed to contaminated eggs, and the prevalence of this pathogen in commercial laying flocks has been identified as a leading epidemiologic risk factor. flock housing and management systems can affect opportunities for the introduction, transmission, and persistence of foodborne pathogens in poultry. the animal welfare implications of different types of housing for laying hens have been widely discussed in recent years, but ...201425306464
bone mineralization of broiler chicks challenged with salmonella enteritidis fed diet containing probiotic (bacillus subtilis).the present study was carried out to determine the effect of probiotic, bacillus subtilis, on ash and calcium contents of tibia bone in unchallenged and challenged broiler chicks with salmonella enteritidis. in a completely randomized design, 160 chicks were divided into four groups. each group had four replicates with 10 birds each. treatments were control group, probiotic-treated group, challenged group and challenged probiotic-treated group. ash and calcium contents of tibia at 21 and 42 days ...201425300924
development of a new molecular subtyping tool for salmonella enterica serovar enteritidis based on single nucleotide polymorphism genotyping using pcr.the lack of a sufficiently discriminatory molecular subtyping tool for salmonella enterica serovar enteritidis has hindered source attribution efforts and impeded regulatory actions required to disrupt its food-borne transmission. the underlying biological reason for the ineffectiveness of current molecular subtyping tools such as pulsed-field gel electrophoresis (pfge) and phage typing appears to be related to the high degree of clonality of s. enteritidis. by interrogating the organism's genom ...201425297333
modeling the effect of temperature on survival rate of salmonella enteritidis in yogurt.the aim of the study was to determine the inactivation rates of salmonella enteritidis in commercially produced yogurt and to generate primary and secondary mathematical models to predict the behaviour of these bacteria during storage at different temperatures. the samples were inoculated with the mixture of three s. enteritidis strains and stored at 5 degrees c, 10 degrees c, 15 degrees c, 20 degrees c and 25 degrees c for 24 h. the number of salmonellae was determined every two hours. it was f ...201425286657
microarray-based detection of salmonella enterica serovar enteritidis genes involved in chicken reproductive tract colonization.salmonella enterica serovar enteritidis has developed the potential to contaminate table eggs internally, by colonization of the chicken reproductive tract and internalization in the forming egg. the serotype enteritidis has developed mechanisms to colonize the chicken oviduct more successfully than other serotypes. until now, the strategies exploited by salmonella enteritidis to do so have remained largely unknown. for that reason, a microarray-based transposon library screen was used to identi ...201425281378
salmonella enterica serovar enteritidis antimicrobial peptide resistance genes aid in defense against chicken innate immunity, fecal shedding, and egg deposition.salmonella enterica serovar enteritidis (s. enteritidis) is a major etiologic agent of nontyphoid salmonellosis in the united states. s. enteritidis persistently and silently colonizes the intestinal and reproductive tract of laying hens, resulting in contaminated poultry products. the consumption of contaminated poultry products has been identified as a significant risk factor for human salmonellosis. to understand the mechanisms s. enteritidis utilizes to colonize and persist in laying hens, w ...201425267840
adhesion of salmonella enteritidis and listeria monocytogenes on stainless steel welds.pathogenic microorganisms are able to adhere on equipment surfaces, being possible to contaminate food during processing. salmonella spp. and listeria monocytogenes are important pathogens that can be transmitted by food, causing severe foodborne diseases. most surfaces of food processing industry are made of stainless steel joined by welds. however currently, there are few studies evaluating the influence of welds in the microorganism's adhesion. therefore the purpose of the present study was t ...201425261827
rapid identification of novel antigens of salmonella enteritidis by microarray-based immunoscreening.we report on an approach to rapidly screen thousands of salmonella enteritidis proteins with the goal of identifying novel immunodominant proteins. we used a microarray-based system that warrants high throughput and easy handling. seven immunogenic candidates were selected after screening. comparative analyses by elisa and microarrays manifested their immunodominant character. the large repetitive protein (sen4030) that plays a role as a putative adhesin in initial cell surface interaction and i ...201425253911
oligomerization of esculin improves its antibacterial activity and modulates antibiotic resistance.in this particular study, the antibacterial activity of esculin and oligomer fractions was assessed. mic values of esculin and its oligomer fractions as well as of some antibiotics against gram-positive and gram-negative strains and against escherichia coli multiresistant variants were determined by the standard broth microdilution method. both esculin and oligoesculin fractions exhibited antibacterial effect against reference strains; staphylococcus aureus, enterococcus faecalis, salmonella ent ...201525248728
an immunogenic salmonella ghost confers protection against internal organ colonization and egg contamination.the tightly regulated expression of the phix174 lysis gene e from a multi-copy plasmid led to the stable production of an salmonella enteritidis bacterial ghost. the present study was conducted to evaluate induction of the humoral and cell-mediated immune responses induced after single or double intramuscular immunization with the s. enteritidis ghost and to assess its protective effect on colonization of the intestinal tract, visceral and reproductive organs, internal egg contamination, and egg ...201425241048
bacteriophage p22 to challenge salmonella in foods.in this study we considered the influence of phage addition on the fate of salmonella enterica serovar typhimurium in different foods. phage p22 was applied to the following: liquid eggs, energy drinks, whole and skimmed milk, apple juice, chicken breast and chicken mince all spiked with its host, whose growth was monitored for 24 and 48 h at 4 °c. appreciable host inactivation, generally in the order of 2 log cycles, was achieved compared to phage-free controls in all food matrices when 10(4) u ...201425240138
correction for rehman et al., high-quality draft whole-genome sequences of 162 salmonella enterica subsp. enterica serovar enteritidis strains isolated from diverse sources in canada. 201425237031
saccharomyces boulardii prevention of the hepatic injury induced by salmonella enteritidis infection.salmonella enterica subsp. enterica serovar enteritidis (salmonella enteritidis) is the predominant cause of serovar-associated food-borne outbreaks in many countries and causes significant clinical symptoms of liver injury, enteritis, and diarrheal diseases. saccharomyces boulardii is used in clinical application for prophylaxis and the treatment of a variety of diseases caused by bacterial infection. we used a mouse model of salmonella enteritidis infection, which included pretreatment with s. ...201425231732
comparative evaluation of salmonella enteritidis ghost vaccines with a commercial vaccine for protection against internal egg contamination with salmonella.the study was conducted for the comparative evaluation of the vaccine potential of salmonella enteritidis (s. enteritidis, se) ghost, se ghost carrying escherichia coli heat labile enterotoxin b subunit (ltb) protein, and a commercial vaccine. group a chickens were used as a non-vaccinated control, group b chickens were immunized with the ghost carrying ltb protein, group c chickens were immunized with the ghost and, group d chickens were immunized with a commercial vaccine. group d chickens sho ...201425218296
membrane lipid composition and stress/virulence related gene expression of salmonella enteritidis cells adapted to lactic acid and trisodium phosphate and their resistance to lethal heat and acid stress.this study evaluated the acid and heat resistance of salmonella enteritidis in simulated gastric fluid (ph 2.0) and during thermal treatment (54-60 °c), respectively, after adaptation to lactic acid (la) or trisodium phosphate (tsp) at various phs (ph 5.3-9.0). the changes in membrane lipid composition and expression levels of rpos and rpoh were examined to elucidate their roles in bacterial stress resistance. transcriptional profile of several virulence-related genes was also analyzed. results ...201425217722
passive protection against salmonella enterica serovar enteritidis infection from maternally derived antibodies of hens vaccinated with a ghost vaccine.this study evaluated maternal immunity against salmonella enterica serovar enteritidis acquired through the egg yolk. two-hundred 19-week-old specific pathogen free (spf) broiler breeders which were randomly divided into two groups of equal size were injected with s. enteritidis ghosts (5 × 10(9) colony forming units in 0.1 ml per hen) and phosphate-buffered saline (pbs, 0.01 mol ⋅ l(-1), ph 7.4) twice, respectively, with an interval of 2 weeks. an indirect enzyme-linked immunosorbent assay (eli ...201425200368
prevalence of salmonella isolates and antimicrobial resistance in poultry meat from south korea.contamination of salmonella was assessed in duck and chicken meat collected from supermarkets, traditional markets, internet shopping malls, and wholesale markets in jeonlado, south korea, in 2013. salmonella contamination was found in 51.3% of duck meat samples and 3.7% of chicken meat samples. salmonella contamination of duck meat samples differed by meat type, i.e., 69.8% of samples of whole ducks and 33.9% of samples of duck pieces. six serotypes were identified from 64 salmonella isolates i ...201425198850
survey of salmonella contamination in chicken layer farms in three caribbean countries.this study was conducted to investigate the demography, management, and production practices on layer chicken farms in trinidad and tobago, grenada, and st. lucia and the frequency of risk factors for salmonella infection. the frequency of isolation of salmonella from the layer farm environment, eggs, feeds, hatchery, and imported day-old chicks was determined using standard methods. of the eight risk factors (farm size, age group of layers, source of day-old chicks, vaccination, sanitation prac ...201425198837
growth and survival of enterobacteriaceae and inoculated salmonella on walnut hulls and maturing walnut fruit.postharvest contamination of in-shell walnuts may occur when the fruit is dropped to or harvested from the orchard floor or as the outer hull is removed with mechanical abrasion and water. to evaluate the effect of maturity on the potential for microbial contamination, 'howard' walnut fruits were collected weekly from the tree canopy, from 6 to 7 weeks before to 1 week after typical commercial harvest. the numbers of microorganisms able to form colonies on plate count agar, macconkey agar (presu ...201425198836
deciphering why salmonella gallinarum is less invasive in vitro than salmonella enteritidis.salmonella gallinarum and salmonella enteritidis are genetically closely related however associated with different pathologies. several studies have suggested that s. gallinarum is less invasive in vitro than s. enteritidis. in this study we confirm that the s. gallinarum strains tested were much less invasive than the s. enteritidis strains tested in cells of avian or human origin. in addition, the s. gallinarum t3ss-1-dependent ability to invade host cells was delayed by two to three hours com ...201425175996
removal of salmonella enterica enteritidis and escherichia coli from green peppers and melons by ultrasound and organic acids.the aim of this study was to evaluate the effectiveness of ultrasound treatment combined with organic acids in the decontamination step for green peppers and melons. the influence of the surface roughness of the peppers and melons on bacterial adhesion was evaluated, as measured using a profilometer. the adhesion of salmonella enterica serovar enteritidis and escherichia coli to the green pepper and melon surfaces was also evaluated by measuring the hydrophobicity of the microorganisms and the s ...201425173914
different clinical presentation of community-onset bacteremia among human immunodeficiency virus-infected and human immunodeficiency virus-uninfected adults in the ed.the objective of this study is to analyze the differences in clinical presentation and outcome of community-onset bacteremia between human immunodeficiency virus (hiv)-infected adults and hiv-uninfected adults visiting the emergency department (ed).201425171801
monitoring of behavior using a video-recording system for recognition of salmonella infection in experimentally infected growing pigs.behavior is one of the most commonly used indicators of illness; however, few studies have investigated how different common diseases affect animal behavior. this experiment was conducted to investigate behavioral and clinical alterations in growing pigs experimentally infected with salmonella spp. during a 4-week post-infection period. a total of 48 growing pigs were divided into one of the three treatment groups (1) control, (2) infection with salmonella typhimurium or (3) infection with salmo ...201525166898
high resolution assembly and characterization of genomes of canadian isolates of salmonella enteritidis.there is a need to characterize genomes of the foodborne pathogen, salmonella enterica serovar enteritidis (se) and identify genetic information that could be ultimately deployed for differentiating strains of the organism, a need that is yet to be addressed mainly because of the high degree of clonality of the organism. in an effort to achieve the first characterization of the genomes of se of canadian origin, we carried out massively parallel sequencing of the nucleotide sequence of 11 se isol ...201425156331
anti-inflammatory activity of animal oils from the peruvian amazon.animal oils and fats from the fishes electrophorus electricus and potamotrygon motoro, the reptiles boa constrictor, chelonoidis denticulata (geochelone denticulata) and melanosuchus niger and the riverine dolphin inia geoffrensis are used as anti-inflammatory agents in the peruvian amazon. the aim of the study was to assess the topic anti-inflammatory effect of the oils/fats as well as to evaluate its antimicrobial activity and fatty acid composition.201425150527
investigating the physiological roles of low-efficiency d-mannonate and d-gluconate dehydratases in the enolase superfamily: pathways for the catabolism of l-gulonate and l-idonate.the sequence/function space in the d-mannonate dehydratase subgroup (mand) of the enolase superfamily was investigated to determine how enzymatic function diverges as sequence identity decreases [wichelecki, d. j., et al. (2014) biochemistry 53, 2722-2731]. that study revealed that members of the mand subgroup vary in substrate specificity and catalytic efficiency: high-efficiency (kcat/km = 10(3)-10(4) m(-1) s(-1)) for dehydration of d-mannonate, low-efficiency (kcat/km = 10-10(2) m(-1) s(-1)) ...201425145794
antibacterial activity of leaf essential oil and its constituents from cinnamomum longepaniculatum.staphylococcus aureus atcc 25923, escherichia coli atcc 25922 and salmonella enteritidis cmcc (b) 50041, were used in the antibacterial tests of cinnamomum longepaniculatum leaf essential oil and its five chemical constituents. the effect of 1, 8-cineole on the ultrastructural structure of the bacteria (s. aureus and e. coli) was also investigated by transmission electron microscopy. the c. longepaniculatum leaf essential oil and the five chemical constituents showed variable levels of inhibitio ...201425126170
quantification of polyphenols and evaluation of antimicrobial, analgesic and anti-inflammatory activities of aqueous and acetone-water extracts of libidibia ferrea, parapiptadenia rigida and psidium guajava.vast numbers of plant species from northeastern brazil have not yet been phytochemically or biologically evaluated.201425124277
nontyphoidal cardiac salmonellosis: two case reports and a review of the literature.nontyphoidal salmonella, especially salmonella enterica, is a rare cause of endocarditis and pericarditis that carries a high mortality rate. proposed predisposing conditions include immunodeficiency states, congenital heart defects, and cardiac valve diseases. we present 2 cases of cardiovascular salmonellosis. the first case is that of a 73-year-old woman with mechanical mitral and bioprosthetic aortic valves who died from sequelae of nontyphoidal salmonella mitral valve vegetation, aortic val ...201425120393
induction of viable but non-culturable (vbnc) state in salmonella cultured in m9 minimal medium containing high glucose.an environmental isolate of salmonella enterica serovar enteritidis (se) clone, se cl#15-1, loses its culturability during 72-h culture in m9 minimal medium containing 0.8% glucose, a concentration twice higher than that in normal m9 medium, whereas the bacterium retains its culturability in normal m9 medium. live/dead analysis using the 5-cyano-2,3-di(p-tolyl) tetrazolium chloride (ctc)-reduction assay revealed that se cells cultured in m9 medium containing 0.8% glucose died with time when in t ...201425109306
screening of lactobacillus strains of domestic goose origin against bacterial poultry pathogens for use as probiotics.lactobacilli are natural inhabitants of human and animal mucous membranes, including the avian gastrointestinal tract. recently, increasing attention has been given to their probiotic, health-promoting capacities, among which their antagonistic potential against pathogens plays a key role. a study was conducted to evaluate probiotic properties of lactobacillus strains isolated from feces or cloacae of domestic geese. among the 104 examined isolates, previously identified to the species level by ...201425104766
prevalence, characterization and antibiotic resistance of salmonella isolates in large corvid species of europe and north america between 2010 and 2013.it is well understood that salmonella is carried by animals and in majority of cases as asymptomatic hosts. surveillance efforts have focused on the role of agriculture and contamination points along the food chain as the main source of human infection; however, very little attention has been paid to the contribution of wildlife in the dissemination of salmonella and what effect anthropogenic sources have on the circulation of antibiotic resistant salmonella serovars in wildlife species. a purpo ...201525103698
assessing the effects of exposure to environmental stress on some functional properties of bifidobacterium animalis ssp. lactis.this study assessed the effects of exposing a strain of bifidobacterium animalis ssp. lactis to acid, bile and osmotic stresses on antagonistic properties, biofilm formation and antibiotic susceptibility/resistance profile. exposure to each stress factor appeared to have no significant effect on the antagonism against escherichia coli nctc 12900 and salmonella enterica serovar enteritidis pt4. no suppression in biofilm formation due to exposure to stress was observed. bile and osmotic stresses r ...201425097108
development of ssdna aptamers for the sensitive detection of salmonella typhimurium and salmonella enteritidis.salmonella enterica subsp. enterica ser. enteritidis and salmonella enterica subsp. enterica ser. typhimurium are the most common and severe food-borne pathogens responsible for causing salmonellosis in humans and animals. the development of an early and ultra-sensitive detection system is the first critical step in controlling this disease. to accomplish this, we used the cell systematic evolution of ligands by exponential enrichment (cell-selex) technique to identify single-stranded dna (ssdna ...201425096391
liver abscess caused by ctx-m-55-type extended-spectrum β-lactamase (esbl)-producing salmonella enteritidis.liver abscesses secondary to salmonella species are rarely described in the general population. we herein describe a case of a liver abscess caused by ctx-m-55-type extended-spectrum β-lactamase (esbl)-producing salmonella enteritidis, which has not been reported in the literature. a 54-year-old male was admitted due to a high fever and was clinically diagnosed with a liver abscess. culture of the fluid from the liver abscess revealed ctx-m-55-type esbl-producing s. enteritidis. although the pat ...201425088889
short communication: combined antimicrobial activity of reuterin and diacetyl against foodborne pathogens.reuterin (β-hydroxypropionialdehyde) is a broad-spectrum antimicrobial substance produced by some strains of lactobacillus reuteri during anaerobic fermentation of glycerol. some of these strains are able to survive and produce reuterin in cheese and yogurt when added as adjuncts to the starter. similarly, in fermented dairy foods, other inhibitory compounds such as lactic acid and diacetyl are produced during fermentation. in this work, we studied the combined effect of reuterin and diacetyl un ...201425087026
precise characterization method of antibody-conjugated magnetic nanoparticles for pathogen detection using stuffer-free multiplex ligation-dependent probe amplification.antibody-conjugated magnetic nanoparticles (ab-mnps) have potential in pathogen detection because they allow target cells to be easily separated from complex sample matrices. however, the sensitivity and specificity of pathogen capture by ab-mnps generally vary according to the types of mnps, antibodies, and sample matrices, as well as preparation methods, including immobilization. therefore, achieving a reproducible analysis utilizing ab-mnps as a pathogen detection method requires accurate cha ...201425070923
trs-based pcr as a potential tool for inter-serovar discrimination of salmonella enteritidis, s. typhimurium, s. infantis, s. virchow, s. hadar, s. newport and s. anatum.salmonella enterica subsp. enterica comprises a number of serovars, many of which pose an epidemiological threat to humans and are a worldwide cause of morbidity and mortality. most reported food infection outbreaks involve the serovars salmonella enteritidis and salmonella typhimurium. rapid identification to determine the primary sources of the bacterial contamination is important to the improvement of public health. in recent years, many dna-based techniques have been applied to genotype salm ...201425063578
rapid whole-genome sequencing for surveillance of salmonella enterica serovar enteritidis.for salmonella enterica serovar enteritidis, 85% of isolates can be classified into 5 pulsed-field gel electrophoresis (pfge) types. however, pfge has limited discriminatory power for outbreak detection. although whole-genome sequencing has been found to improve discrimination of outbreak clusters, whether this procedure can be used in real-time in a public health laboratory is not known. therefore, we conducted a retrospective and prospective analysis. the retrospective study investigated isola ...201425062035
evaluation of anti-se bacteriophage as feed additives to prevent salmonella enteritidis (se) in broiler.this experiment was conducted to evaluate anti-salmonella enteritidis (anti-se) bacteriophage as feed additives to prevent salmonella enteritidis in broilers. the experimental diets were formulated for 2 phases feeding trial, and 3 different levels (0.05, 0.1 and 0.2%) of anti-se bacteriophage were supplemented in basal diet. the basal diet was regarded as the control treatment. a total of 320 1-d-old male broilers (ross 308) were allotted by randomized complete block (rcb) design in 8 replicate ...201325049801
development of recombinant vaccines in lactobacilli for elimination of salmonella.many lactobacillus and lactococcus strains are generally regarded as safe for consumption because they are utilized for food fermentation or inhabit the intestinal mucosa as commensals. recently, vaccine delivery systems using lactic acid bacteria (lab) have been under development. our research group has been investigating the development of oral mucosal vaccines against salmonella enterica serovar enteritidis (se) using lactobacillus casei igm393 as an antigen delivery vehicle. recombinant lact ...201125045314
till receipts--a new approach for investigating outbreaks? evaluation during a large salmonella enteritidis phage type 14b outbreak in a north west london takeaway restaurant, september 2009.selecting suitable controls for outbreak investigations is often difficult and if done inappropriately will lead to biased inferences. till receipts and other sales records are frequently available on food premises, but their applicability has not been fully explored. using data from an investigation into a salmonella outbreak affecting 66 individuals exposed in a london takeaway restaurant, this study aimed to evaluate the use of till receipts to assess associations between sales and illness. c ...201425033053
composition, antioxidant and antimicrobial activities of methanol extracts of some acinos miller species.gc and gc/ms analyses of the methanol extracts obtained from the aerial parts of six acinos miller species from serbia and montenegro were performed. seventy-four constituents, accounting for 84.9-99.0% of the total composition of the extracts, were identified. the common feature of the a. suaveolens, a. majoranifolius and a. alpinus methanol extracts was the high content of monoterpenes, while the common feature of the a. graveolens, a. arvensis and a. hungaricus methanol extracts was the preva ...201425026735
[diversity of human salmonella isolates in the south moravian region in 2009-2012].to characterize the diversity of human salmonella isolates from one of the administrative regions of the czech republic in a time horizon of four years using different typing methods.201425025676
[demarcation of infection with salmonella typhi and s. paratyphi against salmonella enteritidis in children].typhoid and paratyphoid infections (stp) are an important differential diagnosis for salmonella enteritidis infections (nts). the aim of this study is to carve out clinical differences between both infections.201425010129
occurrence, genetic characterization and antimicrobial resistance of salmonella isolated from chicken meat and giblets.summary this study was undertaken to survey the presence of salmonella in 200 chicken samples collected from mansoura, egypt. salmonella was detected in 16% (8/50), 28% (14/50), 32% (16/50) and 60% (30/50) of whole chicken carcasses, drumsticks, livers and gizzards, respectively, with an overall prevalence of 34% (68/200) among all samples. one hundred and sixty-six isolates were identified biochemically as salmonella, and confirmed genetically by pcr, based on the presence of inva and stn genes ...201525004116
glycerol supplementation enhances the protective effect of dietary floramax-b11 against salmonella enteritidis colonization in neonate broiler chickens.two independent trials were conducted in the present study to evaluate the effect of 5% glycerol supplementation combined with dietary floramax-b11 (fm) against salmonella enteritidis colonization in neonate broiler chickens. in each trial, 60 chicks were randomly assigned into 4 groups. group 1 received a control diet. group 2 received a control diet supplemented with 5% glycerol. group 3 received a control diet supplemented with fm, and group 4 received a control diet supplemented with 5% glyc ...201425002551
estimating the burden of acute gastrointestinal illness in grenada.this is the first study conducted in grenada, with a population of approximately 108,000, to quantify the magnitude, distribution, and burden of self-reported acute gastroenteritis (age). a retrospective population survey was conducted in october 2008 and april 2009 and a laboratory survey from october 2008 to september 2009. the estimated monthly prevalence of age was 10.7% (95% ci 9.0-12.6; 1.4 episodes/ person-year), with a median of 3 days of illness. of those who reported age, 31% sought me ...201324992809
a dual mechanism involved in membrane and nucleic acid disruption of avbd103b, a new avian defensin from the king penguin, against salmonella enteritidis cvcc3377.the food-borne bacterial gastrointestinal infection is a serious public health threat. defensins are evolutionarily conserved innate immune components with broad-spectrum antibacterial activity that do not easily induce resistance. avbd103b, an avian defensin with potent activity against salmonella enteritidis, was isolated from the stomach contents of the king penguin (aptenodytes patagonicus). to elucidate further the antibacterial mechanism of avbd103b, its effect on the s. enteritidis cvcc33 ...201424981062
purification of a novel chitin-binding lectin with antimicrobial and antibiofilm activities from a bangladeshi cultivar of potato (solanum tuberosum).a new chitin-binding lectin was purified from a bangladeshi cultivar 'deshi' of potato (solanum tuberosum l.) through anion-exchange and affinity chromatographies using a chitin column. sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) showed the molecular mass of the lectin as 20,000 daltons. this molecular mass was almost half of the molecular masses of chitin-binding lectins derived from other potatoes. the lectin showed both bactericidal and growth-inhibiting activities a ...201424980018
a colonisation-inhibition culture consisting of salmonella enteritidis and typhimurium δhilassraflig strains protects against infection by strains of both serotypes in broilers.consumption of contaminated poultry meat is still an important cause of salmonella infections in humans and there is a need for control methods that protect broilers from day-of-hatch until slaughter age against infection with salmonella. colonisation-inhibition, a concept in which a live salmonella strain is orally administered to day-old chickens and protects against subsequent challenge, can potentially be used as control method. in this study, the efficacy of a salmonella typhimurium δhilass ...201424975814
quinolone-resistant salmonella enterica serotype enteritidis infections associated with international travel.we found a strong association between nalidixic acid-resistant salmonella enterica serotype enteritidis infections in the united states and recent international travel by linking salmonella enteritidis data from the national antimicrobial resistance monitoring system and the foodborne diseases active surveillance network.201424973311
improving the efficiency of transposon mutagenesis in salmonella enteritidis by overcoming host-restriction barriers.transposon mutagenesis using transposome complex is a powerful method for functional genomics analysis in diverse bacteria by creating a large number of random mutants to prepare a genome-saturating mutant library. however, strong host restriction barriers can lead to limitations with species- or strain-specific restriction-modification systems. the purpose of this study was to enhance the transposon mutagenesis efficiency of salmonella enteritidis to generate a larger number of random insertion ...201424973023
development and testing of external quality assessment samples for salmonella detection in poultry samples.salmonella-contaminated poultry house dust plus 10 g chicken faeces inoculated with salmonella enteritidis and then frozen for storage and transport were used as candidate external quality assurance test samples. variations in faeces sample preparation, storage and culture were examined initially. this indicated that, within modest limits, the age of the inoculating culture and of the faeces did not affect detection, nor did swirling the pre-enrichment culture or extending its duration. under op ...201424964747
salmonella enterica serovar enteritidis, england and wales, 1945-2011.in england and wales, the emergence of salmonella enterica serovar enteritidis resulted in the largest and most persistent epidemic of foodborne infection attributable to a single subtype of any pathogen since systematic national microbiological surveillance was established. we reviewed 67 years of surveillance data to examine the features, underlying causes, and overall effects of s. enterica ser. enteritidis. the epidemic was associated with the consumption of contaminated chicken meat and egg ...201424960614
soft cheese supplemented with black cumin oil: impact on food borne pathogens and quality during storage.black cumin seed oil (bcso) was tested for its inhibitory effect against some pathogenic bacteria (staphylococcus aureus atcc 6538, escherichia coli atcc 8739, listeria monocytogenes scott a and salmonella enteritidis pt4) in domiati cheese during cold storage. physical, chemical and sensorial changes in cheese during storage were recorded. pasteurized milk was inoculated before renneting with a mixed culture of bacteria at ca. 4 log cfu ml(-1). in vitro and in situ supplementation with bcso sho ...201424955014
serovar distribution, antimicrobial resistance profiles, and pfge typing of salmonella enterica strains isolated from 2007-2012 in guangdong, china.salmonella enterica includes the major serovars associated with human salmonellosis. in this study, 1764 clinical salmonella enterica isolates from diarrhea outpatients were collected from fifteen cities in guangdong province, china, between 2007 and 2012. these isolates represent all of the salmonella isolates collected from the province during that period.201424939394
identification and characterization of lactic acid bacteria in a commercial probiotic culture.the aim of the present study was to describe the identification and characterization (physiological properties) of two strains of lactic acid bacteria (lab 18 and 48) present in a commercial probiotic culture, floramax(®)-b11. isolates were characterized morphologically, and identified biochemically. in addition, the midi system id, the biolog id system, and 16s rrna sequence analyses for identification of lab 18 and lab 48 strains were used to compare the identification results. tolerance and r ...201424936379
recovery of salmonella serovar enteritidis from inoculated broiler hatching eggs using shell rinse and shell crush sampling methods.this study compared the recovery of salmonella from hatching eggs using 3 sampling methods (eggshell rinsing, eggshell crush following a previous rinse, and eggshell crush without previous rinse). eggshells were drop-inoculated with approximately 10(1), 10(2), or 10(3) cfu/eggshell of salmonella enteritidis and allowed to dry at room temperature for 1 or 24 h. for the shell rinse groups, each inoculated egg was rinsed with buffered peptone water. these rinsed eggs were used for the shell crush w ...201424931964
assessment of the risk of salmonellosis from internally contaminated shell eggs following initial storage at 18 °c (65 °f), compared with 7 °c (45 °f).in the u.s., chicken-breeder farms that supply hatcheries typically store and transport eggs intended for broiler production at a temperature of 18.3 °c (65 °f). however, in case of surplus, some of these eggs may be diverted to human consumption. according to the u.s. food and drug administration's 'egg safety final rule,' shell eggs intended for human consumption are required to be held or transported at or below 7.2 °c (45 °f) ambient temperature beginning 36 h after time of lay. we adapted a ...201424929877
evaluation of indirect impedance for measuring microbial growth in complex food matrices.the suitability of indirect impedance to accurately measure microbial growth in real food matrices was investigated. a variety of semi-solid and liquid food products were inoculated with bacillus cereus, listeria monocytogenes, staphylococcus aureus, lactobacillus plantarum, pseudomonas aeruginosa, escherichia coli, salmonella enteriditis, candida tropicalis or zygosaccharomyces rouxii and co2 production was monitored using a conductimetric (don whitely r.a.b.i.t.) system. the majority (80%) of ...201424929710
evaluation of viability-qpcr detection system on viable and dead salmonella serovar enteritidis.the propidium monoazide (pma) coupled with pcr (viability pcr) is used in foodborne pathogen detection in order to detect only viable bacteria. originally presented to fully remove the signal of dead bacteria, the limits of the viability pcr rapidly came out in the literature. in this study, the use of pma in a viability-qpcr (v-qpcr) was assessed on viable and dead cells of salmonella enterica subsp. enterica serovar enteritidis. the pma treatment protocol was modified (dark incubation duration ...201424927988
molecular characterization and antibiotic resistance of salmonella in children with acute gastroenteritis in abuja, nigeria.in nigeria, acute gastroenteritis in children under five years of age is a major cause of mortality and morbidity; identification and characterization of microbial agents of acute gastroenteritis, including salmonella, remains a powerful tool for effective management, surveillance, and control.201424916869
antibacterial activity of silver nanoparticles: sensitivity of different salmonella serovars.salmonella spp. is one of the main causes of foodborne illnesses in humans worldwide. consequently, great interest exists in reducing its impact on human health by lowering its prevalence in the food chain. antimicrobial formulations in the form of nanoparticles exert bactericidal action due to their enhanced reactivity resultant from their high surface/volume ratio. silver nanoparticles (agnps) are known to be highly toxic to gram-negative and gram-positive microorganisms, including multidrug r ...201424904542
effect of cinnamon (cinnamomum zeylanicum) essential oil supplementation on lamb growth performance and meat quality characteristics.a trial was conducted to examine the effect of cinnamon essential oil supplementation on lamb growth performance and meat quality. sixteen male lambs were randomly assigned to two groups. the first group served as control and was given a basal diet, and the second group was given the same diet supplemented with cinnamon oil (1 ml/kg of concentrated feed) for 35 days. incorporation of cinnamon oil did not affect growth performance (p>0.05). meat ph, colour, water-holding capacity, shear force, in ...201424902083
application of φsp-1 and φsp-3 as a therapeutic strategy against salmonella enteritidis infection using caenorhabditis elegans as model organism.the potential of salmonella-specific phages φsp-1 and φsp-3 as biocontrol agents was studied in vitro, employing host cell lysis test and in vivo, using caenorhabditis elegans as a model organism. for in vivo testing, stage 4 c. elegans larvae were experimentally infected with the pathogen salmonella. worm mortality was scored for 10 days. td50 (the time required for 50% of the nematodes to die) of infected worms in the presence of bacteriophages was comparable to uninfected worms, and the two p ...201424893888
molecular typing of salmonella enterica serovar enteritidis isolates from food-producing animals in japan by multilocus variable-number tandem repeat analysis: evidence of clonal dissemination and replacement.salmonella enterica serovar enteritidis is a zoonotic pathogen. human infections are associated with contaminated eggs and egg products. in japan, since 1989, the incidence of food-borne disease caused by s. enteritidis has increased and a pandemic has occurred; however, little is known about changes that occurred before and after this pandemic event in the dominant lineage of isolates from food-producing animals. this study aimed to determine the s. enteritidis lineages in japan over the last f ...201424886658
altered formalin-induced pain and fos induction in the periaqueductal grey of preadolescent rats following neonatal lps exposure.animal and human studies have demonstrated that early pain experiences can produce alterations in the nociceptive systems later in life including increased sensitivity to mechanical, thermal, and chemical stimuli. however, less is known about the impact of neonatal immune challenge on future responses to noxious stimuli and the reactivity of neural substrates involved in analgesia. here we demonstrate that rats exposed to lipopolysaccharide (lps; 0.05 mg/kg ip, salmonella enteritidis) during pos ...201424878577
characterization of adaptive immune responses induced by a new genetically inactivated salmonella enteritidis vaccine.the superior conservation of antigenic determinants on the surface of genetically inactivated bacterial ghosts makes them attractive immunogenic inactivated vaccine candidates. the efficacy of salmonella enteritidis (se) ghost vaccination was evaluated in chickens by characterizing the nature of the adaptive immune response. chickens from the immunized group demonstrated significant increases in se-specific plasma igg, intestinal secretory iga, and lymphocyte proliferative response. the populati ...201424878070
molecular clonality and detection of class 1 integron in multidrug-resistant salmonella enterica isolates from animal and human in iran.a total of 70 multidrug-resistant (mdr) salmonella isolates (44 human and 26 poultry) were examined. the conserved segment-pcr, restriction fragment length polymorphism-pcr analysis, and dna sequencing were used to determine the presence and cassette content of integrons. the genetic relatedness among the isolates was examined by pulsed-field gel electrophoresis (pfge). the rate of integron carriage for mdr salmonella isolates was 91.4% and integron-positive isolates belonged to six distinct ser ...201424866249
multistate outbreak of salmonella enterica serotype enteritidis infection associated with pet guinea pigs.salmonella causes about one million illnesses annually in the united states. although most infections result from foodborne exposures, animal contact is an important mode of transmission. we investigated a case of salmonella enterica serotype enteritidis (se) sternal osteomyelitis in a previously healthy child who cared for two recently deceased guinea pigs (gps). a case was defined as se pulsed-field gel electrophoresis (pfge) xbai pattern jegx01.0021, blni pattern jega26.0002 (outbreak strain) ...201424866204
inhibitory effects of citral, cinnamaldehyde, and tea polyphenols on mixed biofilm formation by foodborne staphylococcus aureus and salmonella enteritidis.biofilms are significant hazards in the food industry. in this study, we investigated the effects of food additive such as citral, cinnamaldehyde, and tea polyphenols on mixed biofilm formation by foodborne staphylococcus aureus and salmonella serotype enteritidis. the adhesion rates of mixed strains in sub-mic of additives were determined by a microtiter plate assay and bacterial communication signal autoinducer 2 (ai-2) production via a bioluminescence reporter vibrio harveyi bb170. the struct ...201424853514
plasmid profile and pulsed-field gel electrophoresis analysis of salmonella enterica isolates from humans in turkey.this study was conducted for typing salmonella enterica subspecies enterica strains in turkey using pulsed-field gel electrophoresis (pfge) and plasmid dna profile analysis. fourty-two strains were isolated from clinical samples obtained from unrelated patients with acute diarrhea. the samples were collected from state hospitals and public health laboratories located at seven provinces in different regions of turkey at different times between 2004 and 2010. the strains were determined to belong ...201424852084
[native valve endocarditis due to salmonella enteritidis. a cure with medical treatment]. 201424844193
characterization of the invasiveness of monophasic and aphasic salmonella typhimurium strains in 1-day-old and point-of-lay chickens.egg-related outbreaks of salmonellosis are a significant health concern. although salmonella enteritidis (se) is the major egg-associated serotype, salmonella typhimurium (st) can also infect the hen's reproductive tract and contaminate eggs. recently, monophasic and aphasic variants of st have been reported with increased frequency in europe, and the isolation of these variants from laying flocks triggers the same legislative restrictions associated with biphasic st strains. however, little is ...201424802091
control of salmonella enteritidis in turkeys using organic acids and competitive exclusion product.to evaluate the use of organic acids (oas) and competitive exclusion (ce) product administered continuously in the feed and transiently in drinking water on the control of salmonella enterica subspecie enterica serotype enteritidis (se) prior to slaughter.201424797347
a genome-wide screen identifies salmonella enteritidis lipopolysaccharide biosynthesis and the htra heat shock protein as crucial factors involved in egg white persistence at chicken body temperature.eggs contaminated with salmonella enteritidis are an important source of human foodborne salmonella infections. salmonella enteritidis is able to contaminate egg white during formation of the egg within the chicken oviduct, and it has developed strategies to withstand the antimicrobial properties of egg white to survive in this hostile environment. the mechanisms involved in the persistence of salmonella enteritidis in egg white are likely to be complex. to address this issue, a microarray-based ...201424795321
marinade with thyme and orange oils reduces salmonella enteritidis and campylobacter coli on inoculated broiler breast fillets and whole wings.essential oils have been reported to possess antimicrobial properties and therefore have potential usage as natural antimicrobials in food. in a previous study, thyme orange essential oil combination (toc) used at the 0.5% level as a dip application on chicken cut-up parts had a significant antibacterial effect against salmonella and campylobacter. a study was designed to evaluate the effect of salt-phosphate marinade solution containing 0.5% toc to 1) reduce salmonella enteritidis and campyloba ...201424795320
characterization of multidrug-resistant salmonella enterica serovars indiana and enteritidis from chickens in eastern china.a total of 310 salmonella isolates were isolated from 6 broiler farms in eastern china, serotyped according to the kauffmann-white classification. all isolates were examined for susceptibility to 17 commonly used antimicrobial agents, representative isolates were examined for resistance genes and class i integrons using pcr technology. clonality was determined by pulsed-field gel electrophoresis (pfge). there were two serotypes detected in the 310 salmonella strains, which included 133 salmonell ...201424788434
high-quality draft whole-genome sequences of 162 salmonella enterica subsp. enterica serovar enteritidis strains isolated from diverse sources in canada.we report the high-quality draft genome sequences of 162 strains of salmonella enterica subsp. enterica serovar enteritidis representing diverse phage types and pulsed-field gel electrophoresis (pfge) profiles. the analysis of these genomes will enable the identification of markers that are useful for differentiating strains of this highly clonal serovar and will provide insights into the evolution, virulence, and epidemiology of the strains.201424786953
comparative analysis of virulence and resistance profiles of salmonella enteritidis isolates from poultry meat and foodborne outbreaks in northern jordan.this study was conducted to isolate salmonella enteritidis from poultry samples and compare their virulence and antibiotic resistance profiles to s. enteritidis isolated from outbreaks in northern jordan. two hundred presumptive isolates were obtained from 302 raw poultry samples and were subjected to further analysis and confirmation. a phylogenic tree based on 16s rrna sequencing was constructed and selected isolates representing each cluster were further studied for their virulence in normal ...201424780883
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