Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| pantoea agglomerans as a cause of septic arthritis after palm tree thorn injury; case report and literature review. | we report the case of a 14 year old healthy boy, who was admitted six weeks after being injured by a palm tree thorn, with limping caused by pain and swelling in his right knee. an ultrasound examination revealed a foreign body in the posterior lateral aspect of the right knee. pantoea agglomerans was identified in the synovial fluid. the patient underwent two arthrotomies and was treated with amoxicillin-clavulanate intravenously for three weeks. the postoperative course was uneventful, and joi ... | 2003 | 12765929 |
| structural analysis of the dna-binding domain of the erwinia amylovora rcsb protein and its interaction with the rcsab box. | the transcriptional regulator rcsb interacts with other coactivators to control the expression of biosynthetic operons in enterobacteria. while in a heterodimer complex with the regulator rcsa the rcsab box consensus is recognized, dna binding sites for rcsb without rcsa have also been identified. the conformation of rcsb might therefore be modulated upon interaction with various coactivators, resulting in the recognition of different dna targets. we report the solution structure of the c-termin ... | 2003 | 12740396 |
| quorum sensing in plant-pathogenic bacteria. | quorum sensing (qs) allows bacteria to assess their local population density and/or physical confinement via the secretion and detection of small, diffusible signal molecules. this review describes how phytopathogenic bacteria have incorporated qs mechanisms into complex regulatory cascades that control genes for pathogenicity and colonization of host surfaces. traits regulated by qs include the production of extracellular polysaccharides, degradative enzymes, antibiotics, siderophores, and pigm ... | 2003 | 12730390 |
| bacteriocin serratine-p as a biological tool in the control of fire blight erwinia amylovora. | fire blight, caused by the bacterium erwinia amylovora (burill winslow et al.), is the most important bacterial disease in european pear growing. it can cause a lot of damage in some countries on apple and on pear trees in orchards and also in the fruit tree nurseries. in belgium, the disease is present since 1972. control of fire blight in belgian fruit orchards is made on a broad basis of measurements in and around the fruit trees. the use of an antibiotic is allowed for application only durin ... | 2002 | 12701444 |
| bacteriophages of erwinia amylovora. | fifty bacteriophage isolates of erwinia amylovora, the causal agent of fire blight, were collected from sites in and around the niagara region of southern ontario and the royal botanical gardens, hamilton, ontario. forty-two phages survived the isolation, purification, and storage processes. the majority of the phages in the collection were isolated from the soil surrounding trees exhibiting fire blight symptoms. only five phages were isolated from infected aerial tissue in pear and apple orchar ... | 2003 | 12676693 |
| the hrpx/hrpy two-component system activates hrps expression, the first step in the regulatory cascade controlling the hrp regulon in pantoea stewartii subsp. stewartii. | a regulatory cascade activating hrp/hrc type iii secretion and effector genes was delineated in pantoea stewartii subsp. stewartii, a bacterial pathogen of corn. four hrp regulatory genes were characterized: hrpx and hrpy encode the sensor kinase and response regulator, respectively, of a two-component signal transduction system; hrps encodes an ntrc-like transcriptional enhancer; and hrpl encodes an alternative sigma factor. epistasis analysis, expression studies using gene fusions, and genetic ... | 2003 | 12650455 |
| mathematical modeling of in vitro enzymatic production of 2-keto-l-gulonic acid using nad(h) or nadp(h) as cofactors. | a 2-keto-l-gulonic acid (2-klg) production process using stationary pantoea citrea cells and a corynebacterium 2,5-diketo-d-gluconic acid (2,5-dkg) reductase enzyme has been developed which may represent an improved method of vitamin c biosynthesis. experimental data was collected using the f22y/a272g 2,5-dkg reductase mutant and nadp(h) as a cofactor. an extensive kinetic analysis was performed and a kinetic rate equation model for this process was developed. a recent protein engineering effort ... | 2002 | 12646322 |
| gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in arabidopsis. | the major regulatory shoot signal is auxin, whose synthesis in young leaves has been a mystery. to test the leaf-venation hypothesis [r. aloni (2001) j plant growth regul 20: 22-34], the patterns of free-auxin production, movement and accumulation in developing leaf primordia of dr5::gus-transformed arabidopsis thaliana (l.) heynh. were visualized. dr5::gus expression was regarded to reflect sites of free auxin, while immunolocalization with specific monoclonal antibodies indicated total auxin d ... | 2003 | 12624772 |
| quorum-sensing-directed protein expression in serratia proteamaculans b5a. | n-acyl-l-homoserine-lactone-producing serratia species are frequently encountered in spoiling foods of vegetable and protein origin. the role of quorum sensing in the food spoiling properties of these bacteria is currently being investigated. a set of luxr luxi homologous genes encoding a putative quorum sensor was identified in the n-(3-oxo-hexanoyl)-l-homoserine lactone (3-oxo-c6-hsl)-producing serratia proteamaculans strain b5a. the 3-oxo-c6-hsl synthase spri showed 79 % similarity with esai ... | 2003 | 12624209 |
| respiration of 13c-labeled substrates added to soil in the field and subsequent 16s rrna gene analysis of 13c-labeled soil dna. | our goal was to develop a field soil biodegradation assay using (13)c-labeled compounds and identify the active microorganisms by analyzing 16s rrna genes in soil-derived (13)c-labeled dna. our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soil associated with sampling and laboratory incubation. the new field-based assay involved the release of (13)c-labeled compounds (glucose, phenol, caffeine, and naphthalene) to soil ... | 2003 | 12620850 |
| water activity, temperature, and ph effects on growth of the biocontrol agent pantoea agglomerans cpa-2. | the growth response of the biocontrol agent pantoea agglomerans to changes in water activity (a(w)), temperature, and ph was determined in vitro in nutrient yeast extract-sucrose medium. the minimum temperature at which p. agglomerans was able to grow was 267-272 kelvins (-6 to -1 degrees c), and growth of p. agglomerans did not change at varying ph levels (4.5-8.6). the minimum a(w) for growth was 0.96 in media modified with glycerol and 0.95 in media modified with nacl or glucose. solute used ... | 2002 | 12619821 |
| isolation of a pantoea dispersa-like strain fron a 71-year-old woman with acute myeloid leukemia and multiple myeloma. | 2003 | 12608369 | |
| microbiology of choledochal bile in patients with choledocholithiasis admitted to a tertiary hospital. | the present study was designed to investigate the microbiology of choledochal bile of patients with cholangitis and choledocholithiasis. | 2003 | 12603536 |
| cloning and expression of the gene encoding catalytic subunit of thermostable glucose dehydrogenase from burkholderia cepacia in escherichia coli. | we have cloned a 1620-nucleotide gene encoding the catalytic subunit (alpha subunit) of a thermostable glucose dehydrogenase (gdh) from burkholderia cepacia. the fad binding motif was found in the n-terminal region of the alpha subunit. the deduced primary structure of the alpha subunit showed about 48% identity to the catalytic subunits of sorbitol dehydrogenase (sdh) from gluconobacter oxydans and 2-keto-d-gluconate dehydrogenases (2kgdh) from erwinia herbicola and pantoea citrea. the alpha su ... | 2003 | 12573242 |
| differences between pseudomonas syringae pv. syringae b728a and pantoea agglomerans brt98 in epiphytic and endophytic colonization of leaves. | the leaf colonization strategies of two bacterial strains were investigated. the foliar pathogen pseudomonas syringae pv. syringae strain b728a and the nonpathogen pantoea agglomerans strain brt98 were marked with a green fluorescent protein, and surface (epiphytic) and subsurface (endophytic) sites of bean and maize leaves in the laboratory and the field were monitored to see if populations of these strains developed. the populations were monitored using both fluorescence microscopy and counts ... | 2003 | 12571050 |
| [use transposon and parde through in vivo cloning to promote the genetic stability of plasmid pcpp430]. | plasmid pcpp430 carrying the hrp gene cluster after transformated into 308r (pantoea agglomerans) can cause the hypersensitive response, and simultaneously induce the plant resistance to disease. it was genetically unstable in p. agglomerans. 0.8 kb parde region of the broad-host range plasmid rk2 is responsible for plasmid partition. it can mediate plasmid maitenance of many kinds in rhizobium meliloti, and also can promote the genetic stability of recombinant plasmid in the biocontrol bacteria ... | 2001 | 12552820 |
| quantitative assessment of antigen-specific cd8+ t cells in the mouse: application to vaccine research. | an effective hiv vaccine will likely need to induce potent and broad-based immunity, including cd8+ t cell responses. hence, a quantitative assay to measure such responses in animal models will be important in the evaluation of candidate hiv vaccines. we show here that a single immunization with hiv dna vaccines, followed by challenge with recombinant vaccinia virus expressing the relevant hiv antigen, allows quantitative assessment of cd8+ t cell responses. these responses can be profound (>30% ... | 2003 | 12527230 |
| characterization of five phyllosphere bacteria isolated from rosa rugosa leaves, and their phenotypic and metabolic properties. | five gram-negative bacteria, all of which were enterobacteriaceae, were isolated from the phyllosphere of green or senescing leaves of rosa rugosa, and their phenotypic and physiological characteristics were examined. partial 16s rdna sequences led to identification of these isolates as pantoea agglomerans, klebsiella terrigena, erwinia rhapontici, and two strains of rahnella aquatilis. interestingly, these phyllosphere bacteria had certain phenotypic and physiological convergences, while they s ... | 2002 | 12506991 |
| phylogenetical relationship based on groe genes among phenotypically related enterobacter, pantoea, klebsiella, serratia and erwinia species. | in an attempt to define the phylogenetical relationship among 17 phenotypically related species of genera enterobacter, pantoea, serratia, klebsiella and erwinia, we determined almost all of their groe operon sequences using the polymerase chain reaction direct sequencing method. the number of nucleotide substitutions per site was 0.12+/-0.030. the value was 3.6-fold higher than that of 16s rdna. as a result, we were successful in constructing molecular phylogenetic trees which had a finer resol ... | 1997 | 12501307 |
| erwinia aphidicola, a new species isolated from pea aphid, acyrthosiphon pisum. | five strains of gram-negative, oxidase-negative, facultatively anaerobic, fermentative, motile, rod-shaped bacterium with the general characteristics of the family enterobacteriaceae were isolated from the gut of multiple specimens of the pea aphid. all the strains caused aphid mortality when ingested by insects via a synthetic diet. the results of biochemical tests showed that these strains are most related to erwinia herbicola and pantoea agglomerans. according to dna-dna hybridization, the fi ... | 1997 | 12501306 |
| effect of leaf surface waxes on leaf colonization by pantoea agglomerans and clavibacter michiganensis. | to evaluate the influence of leaf cuticular waxes on bacterial colonization of leaves, bacterial colonization patterns were examined on four glossy maize (zea mays l.) mutants that were altered in their cuticular wax biosynthesis. mutant gl3 was indistinguishable from the wild-type maize in its ability to foster colonization by the two bacterial species, pantoea agglomerans and clavibacter michiganensis subsp. nebraskensis. in contrast, the other three mutants supported the development of popula ... | 2002 | 12481996 |
| preservation of chitinolytic pantoae agglomerans in a viable form by cellular dried alginate-based carriers. | improved viability of gram-negative bacteria during freeze-dehydration, storage, and soil inoculation is of crucial importance to their efficient application. the chitinolytic pantoae (enterobacter) agglomerans strain ic1270, a potential biocontrol agent of soil-borne plant-pathogenic fungi, was used as a model organism to study the efficacy of freeze-dried alginate-based beads (macrocapsules) as possible carriers for immobilized gram-negative bacterial cells. these macrocapsules were produced b ... | 2002 | 12467443 |
| [tibial osteitis caused by pantoea agglomerans after open grade iiib tibial shaft fracture]. | bone infection is not an uncommon complication of open tibial shaft fractures. we report an unusual case of chronic tibial osteitis caused by pantoea agglomerans after an open grade iiib tibial shaft fracture. p. agglomerans, an enterobacteria, was identified on bone samples obtained nine months after the fracture. the infection probably resulted from the initial soil contamination and developed despite fixation and bone covering with a muscle flap performed during the same operative time and ad ... | 2002 | 12447133 |
| tonb of escherichia coli activates fhua through interaction with the beta-barrel. | fhua is a multifunctional protein in the outer membrane of escherichia coli that actively transports fe(3+)-ferrichrome and the antibiotics albomycin and rifamycin cgp 4832, and serves as a receptor for the unrelated phages t5, t1, phi80 and uc-1, colicin m and microcin j25. the energy source for active transport is the proton-motive force of the cytoplasmic membrane, which is required for all fhua functions except infection by phage t5, and is thought to be mediated to the outer-membrane recept ... | 2002 | 12427941 |
| induced systemic resistance of selected endophytic bacteria against meloidogyne incognita on tomato. | in previous work, the four endophytic bacteria pantoea agglomerans mk-29, cedeca davisae mk-30, enterobacter spp. mk-42 and pseudomonas putida mt-19 were shown to reduce meloidogyne incognita on tomato when applied as a seed treatment and/or soil drench. the objective of this work was to study these bacteria for their potential to induce systemic resistance against root knot nematodes on tomato. to guarantee spatial separation between inducing agent and pathogen a split-root system was chosen an ... | 2001 | 12425091 |
| diversity of endophytic bacterial populations and their interaction with xylella fastidiosa in citrus plants. | citrus variegated chlorosis (cvc) is caused by xylella fastidiosa, a phytopathogenic bacterium that can infect all citrus sinensis cultivars. the endophytic bacterial communities of healthy, resistant, and cvc-affected citrus plants were studied by using cultivation as well as cultivation-independent techniques. the endophytic communities were assessed in surface-disinfected citrus branches by plating and denaturing gradient gel electrophoresis (dgge). dominant isolates were characterized by fat ... | 2002 | 12324338 |
| differences in attachment of salmonella enterica serovars and escherichia coli o157:h7 to alfalfa sprouts. | numerous salmonella enterica and escherichia coli o157:h7 outbreaks have been associated with contaminated sprouts. we examined how s. enterica serovars, e. coli serotypes, and nonpathogenic bacteria isolated from alfalfa sprouts grow on and adhere to alfalfa sprouts. growth on and adherence to sprouts were not significantly different among different serovars of s. enterica, but all s. enterica serovars grew on and adhered to alfalfa sprouts significantly better than e. coli o157:h7. e. coli o15 ... | 2002 | 12324317 |
| temporal distribution of chaetocnema pulicaria (coleoptera: chrysomelidae) populations in iowa. | in 1999 and 2000, yellow sticky cards and sweep net samples were used to document the occurrence of an overwintering adult generation of chaetocnema pulicaria melsheimer, corn flea beetle, followed by two distinct populations peaks during the growing season in iowa emergence of the overwintering adult generation started in mid-april and continued until early june in both years, with populations as high as 45 +/- 7.9 per 10 sweeps. periods that ranged from 14 to 32 d were observed in 1999 and 200 ... | 2002 | 12216815 |
| construction and characterization of a prou-gfp transcriptional fusion that measures water availability in a microbial habitat. | we constructed and characterized a transcriptional fusion that measures the availability of water to a bacterial cell. this fusion between the prou promoter from escherichia coli and the reporter gene gfp was introduced into strains of e. coli, pantoea agglomerans, and pseudomonas syringae. the prou-gfp fusion in these bacterial biosensor strains responded in a quantitative manner to water deprivation caused by the presence of nacl, na(2)so(4), kcl, or polyethylene glycol (molecular weight, 8000 ... | 2002 | 12200319 |
| the effect of nitrogen and carbon sources on growth of the biocontrol agent pantoea agglomerans strain cpa-2. | the effect of several nitrogen and carbon sources on the growth of pantoea agglomerans (strain cpa-2) was studied for the first time. | 2002 | 12100585 |
| fitness of salmonella enterica serovar thompson in the cilantro phyllosphere. | the epiphytic fitness of salmonella enterica was assessed on cilantro plants by using a strain of s. enterica serovar thompson that was linked to an outbreak resulting from cilantro. salmonella serovar thompson had the ability to colonize the surface of cilantro leaves, where it was detected by confocal laser scanning microscopy (clsm) at high densities on the veins and in natural lesions. the population sizes of two common colonizers of plant surfaces, pantoea agglomerans and pseudomonas chloro ... | 2002 | 12089050 |
| plant-dependent genotypic and phenotypic diversity of antagonistic rhizobacteria isolated from different verticillium host plants. | to study the effect of plant species on the abundance and diversity of bacterial antagonists, the abundance, the phenotypic diversity, and the genotypic diversity of rhizobacteria isolated from potato, oilseed rape, and strawberry and from bulk soil which showed antagonistic activity towards the soilborne pathogen verticillium dahliae kleb. were analyzed. rhizosphere and soil samples were taken five times over two growing seasons in 1998 and 1999 from a randomized field trial. bacterial isolates ... | 2002 | 12089011 |
| the autoregulatory role of esar, a quorum-sensing regulator in pantoea stewartii ssp. stewartii: evidence for a repressor function. | capsular polysaccharide synthesis and virulence in the plant pathogenic bacterium pantoea stewartii ssp. stewartii requires the quorum-sensing regulatory proteins, esar and esai, and the diffusible inducer n-(3-oxo-hexanoyl)-l-homoserine lactone. prior mutational studies suggested that esar might function as a repressor of quorum sensing in the control of capsular polysaccharide synthesis. further, a lux box-like palindromic sequence coinciding with the putative -10 element of the esar promoter ... | 2002 | 12067349 |
| response of the ice-nucleating bacterium pantoea ananas kuin-3 during cold acclimation. | the ice-nucleating bacterium pantoea ananas kuin-3 accumulated glucose in cells following a shift in temperature (10 degrees c) from the optimum growth temperature (30 degrees c). this accumulation might be caused by the activation of glucose-6-phosphatase. although this strain after culturing at 30 degrees c was harmed by freezing, the cryotolerance of this strain was reached about 80% after cold acclimation at 10 degrees c. | 2002 | 12036064 |
| functional analysis of the erwinia herbicola tutb gene and its product. | the tutb gene, which lies just downstream of tpl, has been cloned from erwinia herbicola, and its product was analyzed. despite its high sequence similarity to tryptophan transporters, tutb was found to be a tyrosine-specific transporter. tryptophan acted as a competitive inhibitor of tyrosine transport. unlike the tryptophanase operon, the tpl and tutb genes do not constitute an operon. | 2002 | 12003958 |
| effect of package and storage conditions on viability and efficacy of the freeze-dried biocontrol agent pantoea agglomerans strain cpa-2. | to reduce concentrations of protective and rehydrating media and to evaluate the effect of storage temperature, packaging and atmosphere conditions on the stability of freeze-dried pantoea agglomerans cells. efficacy against penicillium digitatum of freeze-dried cells in orange fruits was also evaluated. | 2002 | 11972691 |
| a note: gut bacteria produce components of a locust cohesion pheromone. | faecal pellets from germ-free locusts were used as culture media to determine the ability of locust gut bacteria to synthesize phenolic components of the locust cohesion pheromone. | 2002 | 11966918 |
| post-harvest biological control by pantoea agglomerans (cpa-2) on golden delicious apples. | to investigate the potential of pantoea agglomerans to control the major post-harvest diseases on golden delicious apples. | 2002 | 11849352 |
| posttranscriptional activation of the transcriptional activator rob by dipyridyl in escherichia coli. | the transcriptional activator rob consists of an n-terminal domain (ntd) of 120 amino acids responsible for dna binding and promoter activation and a c-terminal domain (ctd) of 169 amino acids of unknown function. although several thousand molecules of rob are normally present per escherichia coli cell, they activate promoters of the rob regulon poorly. we report here that in cells treated with either 2,2"- or 4,4"-dipyridyl (the latter is not a metal chelator), rob-mediated transcription of var ... | 2002 | 11844771 |
| occurrence and transferability of beta-lactam resistance in enterobacteriaceae isolated in children's university hospital in bratislava. | occurrence and transferability of beta-lactam resistance in 30 multi-resistant escherichia coli, klebsiella spp., enterobacter spp., pantoea agglomerans, citrobacter freundii and serratia marcescens strains isolated from children between 0 and 3 years of age is presented. the strains were resistant to ampicillin (30), cefoxitin (22), cefotaxime (30), ceftriaxone (30), ceftazidime (30) and aztreonam (28), but susceptible to cefepime (30) and imipenem (26). twenty-eight of 30 isolates possessed a ... | 2001 | 11830947 |
| survival of pantoea agglomerans strain cpa-2 in a spray-drying process. | spray drying could be a suitable method for preserving microorganisms, as it allows large quantities of cultures to be dried at low cost. the aims of this paper were to evaluate the effects of spray-drying conditions on survival of the biocontrol agent pantoea agglomerans cpa-2, which has shown antifungal activity against penicillium expansum and penicillium digitatum on citrus fruits. various compounds cited in the bibliography as carriers were tested in our spray drying, and some salts (mgso4, ... | 2002 | 11808793 |
| control of penicillium expansum and botrytis cinerea on apples and pears with the combination of candida sake and pantoea agglomerans. | the effectiveness of candida sake (cpa-1) in combination with pantoea agglomerans (cpa-2) for controlling penicillium expansum and botrytis cinerea on pears and apples was determined. the concentrations tested were 2 x 10(6) and 2 x 10(7) cfu/ml for c. sake and 2 x 10(7) and 8 x 10(7) cfu/ml for p. agglomerans. at room temperature, the two antagonists were combined in proportions of 0 to 100% in 25% increments. at the proportion of 50:50, no rot development was observed in pears, and the greates ... | 2002 | 11808791 |
| biological control of postharvest pear diseases using a bacterium, pantoea agglomerans cpa-2. | epiphytic microorganisms isolated from the fruits and leaf surfaces of apples and pears were screened for antagonistic activity against penicillium expansum on pears. from 247 microorganisms tested for antagonistic properties against p. expansum, a bacterium strain identified as pantoea agglomerans (cpa-2) was selected. this bacterium was very effective against botrytis cinerea, p. expansum and rhizopus stolonifer. complete control at the three tested concentrations (2 x 10(7), 8 x 10(7) and 1 x ... | 2001 | 11759762 |
| response of herb processing workers to work-related airborne allergens. | a group of 51 herb processing workers employed in a big herb processing facility located in eastern poland were examined by the skin and precipitin tests with, respectively, 4 and 17 extracts of microorganisms associated with organic dusts. out of this number, 32 workers were examined by the skin test with 7 extracts of selected herbs processed in the facility. all the subjects were asked about the occurrence of work-related symptoms. 32 healthy office workers were examined with microbial extrac ... | 2001 | 11748888 |
| young farmers with cellular reactivity to airborne microbes suffer more frequently from work-related skin symptoms and allergic dermatitis. | 75 farming students (49 males and 26 females aged 16-23 years) underwent dermatological, laryngological and pulmonary examination, skin prick tests with common and farm allergens, phadiatop and total ige measurement. after that, the migration inhibition tests with antigens of airborne microbes typical for farm environment (saccharopolyspora rectivirgula, pantoea agglomerans, and aspergillus fumigatus) were carried out. possible differences between students with positive results and those non-rea ... | 2001 | 11748885 |
| microfloral diversity of cultured and wild strains of psoroptes ovis infesting sheep. | pcr amplification of 16s rdna was used to determine the diversity of bacteria associated with 3 strains of sheep scab mite, psoroptes ovis. eight species of bacteria were identified by phylogenetic analysis of the pcr product sequences. seven of these species are previously unreported in association with sheep scab mites. five species were matched to serratia marcesens, proponibacteium acnes, phyllobacterium rubiacearum, pantoea agglomerans, curacaobacter baltica, whereas the remaining 3 sequenc ... | 2001 | 11719954 |
| crystallization and rhenium mad phasing of the acyl-homoserinelactone synthase esai. | acyl-homoserine-l-lactones (ahls) are diffusible chemical signals that are required for virulence of many gram-negative bacteria. ahls are produced by ahl synthases from two substrates, s-adenosyl-l-methionine and acyl-acyl carrier protein. the ahl synthase esai, which is homologous to the ahl synthases from other pathogenic bacterial species, has been crystallized in the primitive tetragonal space group p4(3), with unit-cell parameters a = b = 66.40, c = 47.33 a. the structure was solved by mul ... | 2001 | 11717525 |
| biological activity of harpin produced by pantoea stewartii subsp. stewartii. | pantoea stewartii subsp. stewartii causes stewart's wilt of sweet corn. a hypersensitive response and pathogenicity (hrp) secretion system is needed to produce water-soaking and wilting symptoms in corn and to cause a hypersensitive response (hr) in tobacco. sequencing of the hrp cluster revealed a putative harpin gene, hrpn. the product of this gene was overexpressed in escherichia coli and shown to elicit the hr in tobacco and systemic resistance in radishes. the protein was designated hrpn(pn ... | 2001 | 11605962 |
| genetic organization of the pantoea stewartii subsp. stewartii hrp gene cluster and sequence analysis of the hrpa, hrpc, hrpn, and wtse operons. | the hrp/wts gene cluster of pantoea stewartii subsp. stewartii is required for pathogenicity on sweet corn and the ability to elicit a hypersensitive response (hr) in tobacco. site-directed transposon mutagenesis and nucleotide sequencing were used to identify hrp/wts genes within the left 20 kb of this cluster. seventeen open reading frames (orfs) comprise seven genetic complementation groups. these orfs share homology with hrp and dsp genes from erwinia amylovora, erwinia chrysanthemi, and pse ... | 2001 | 11605961 |
| the replicon of psw800 from pantoea stewartii. | a 2019 bp dna fragment containing the replicon of psw800 from pantoea stewartii sw2 was cloned and characterized. this replicon contains two genes--repa and repb, which encode a 36.5 kda replication initiation protein (repa) and a peptide of 18 aa, respectively. these two genes overlap by 8 bases with repb situated upstream. the replicon also transcribes an antisense rna (rnai) that inhibits the expression of repa and repb. the ribosome-binding sequence (rbs) of repa is likely to be hidden in a ... | 2001 | 11577155 |
| evaluation of plant growth promoting and colonization ability of endophytic diazotrophs from deep water rice. | a study of the diversity of endophytic bacteria present in seeds of a deepwater rice variety revealed the presence of seven types of box-pcr fingerprints. in order to evaluate the plant growth promoting potential the presence of nitrogenase, indole acetic acid production and mineral phosphate solubilization were estimated in the representative box-pcr types. the seven representatives of box-pcr types produced indole acetic acid, reduced acetylene and showed specific immunological cross-reaction ... | 2001 | 11566385 |
| production of the biocontrol agent pantoea agglomerans strain cpa-2 using commercial products and by-products. | the aim of this paper was to find the nitrogen and carbon sources that provide maximum biomass production of strain cpa-2 of the biocontrol agent pantoea agglomerans and minimum cost of media, whilst maintaining biocontrol efficacy. to reduce the cost of media, commercial products and by-products were tested. p. agglomerans can be produced using a combination of nitrogen sources such as yeast extract (5 g l(-1)) and dry beer yeast (10 g l(-1)) with inexpensive carbohydrates such as sucrose (10 g ... | 2001 | 11549003 |
| intervening sequences in rrl genes and fragmentation of 23s rrna in genera of the family enterobacteriaceae. | intervening sequences (ivss) in the rrl genes for 23s rrna are transcribed but later removed by rnase iii without religation during rna processing, leading to fragmented rrna. we examined about 240 strains of the family enterobacteriaceae for presence of ivss using pcr. no ivss were detected in strains belonging to escherichia, shigella, enterobacter, erwinia, ewingella, hafnia, kluyvera, morganella, pantoea, or serratia. previously unreported ivss were detected in klebsiella oxytoca, citrobacte ... | 2001 | 11544246 |
| molecular identification of microorganisms from endodontic infections. | a relatively wide range of bacteria have been isolated from root canals using standard culture techniques. however, only 50% of the bacteria in the oral cavity are cultivable (s. s. socransky et al., arch. oral biol. 8:278-280, 1963); hence, bacterial diversity in endodontic infections is underestimated. this study used a pcr-based 16s rrna gene assay, followed by cloning and sequencing of 16s rrna amplicons from a small subset of samples to assess the diversity of bacteria present in infected r ... | 2001 | 11526164 |
| comparative antimicrobial spectrum and activity of bms284756 (t-3811, a desfluoroquinolone) tested against 656 enterobacteriaceae, including preliminary in vitro susceptibility test comparisons and development. | bms284756 (t-3811), a novel des-f(6)-quinolone, was evaluated using isolates of enterobacteriaceae from the sentry antimicrobial surveillance program tested by etest (ab biodisk, solna, sweden), reference broth microdilution and disk diffusion (5-microg) methods. ciprofloxacin, levofloxacin, gemifloxacin and gatifloxacin were also tested by broth microdilution as comparator antimicrobial agents within the same drug class. the 656 isolate collection included species from the genera citrobacter, e ... | 2001 | 11516937 |
| samsonia erythrinae gen. nov., sp. nov., isolated from bark necrotic lesions of erythrina sp., and discrimination of plant-pathogenic enterobacteriaceae by phenotypic features. | bacterial strains isolated from diseased erythrina (erythrina sp.) trees in martinique (french west indies) were studied using phenotypic tests, 16s rdna sequence analysis and dna-dna hybridization. numerical analysis of phenotypic characteristics showed that these strains formed an homogeneous phenon among plant-pathogenic enterobacteriaceae, and gave useful and updated information for the identification of these bacteria. results of dna-dna hybridization indicated that strains from erythrina b ... | 2001 | 11491325 |
| identity and antibiotic susceptibility of enterobacterial flora of salad vegetables. | the enterobacterial flora from carrots (organic and non-organic) and salad vegetables has been identified and antibiotic susceptibilities determined. pseudomonas fluorescens and pantoea (formerly enterobacter) agglomerans were the species most commonly found; the former was usually resistant to at least six of the antibiotics under test. rahnella aquatilis (often producing beta-lactamase) was also found in carrots. there were no clear differences in flora from organic and non-organic carrots. th ... | 2001 | 11463532 |
| work-related skin symptoms and type i allergy among eastern-polish farmers growing hops and other crops. | 73 eastern-polish farmers growing hops and other crops were examined. they were questioned by a dermatologist and subsequently skin prick-tested with allergens of hops, grain dust, straw dust, hay dust, storage mites, and antigens of microorganisms typical for farm environment. results: 14 farmers (19.2%) complained of work-related skin symptoms, caused most often by hops (11%), followed by grain (5.6%), hay (5.5%) and straw (4.1%). five farmers (6.8%) complained of hand dermatitis, four (5.5%) ... | 2001 | 11426925 |
| [ability of representatives of pantoea agglomerans, as well as bacillus subtilis and some pseudomonas species to suppress the development of phytopathgenic bacteria and micromycetes in regulating plant growth]. | the ability of representatives of pantoea agglomerans (erwinia herbicola (lohnis) dye [21]), bacillus subtilis and some species of pseudomonas genus to inhibit the growth of phytopathogenic bacteria and micromycetes and to regulate the growth of plants has been comparatively studied. the ability to inhibit the growth of mycellium of phytopathogenic fusarium avenaceum, f. gibbosum, f. oxysporum was found out in all of 13 investigated strains of p. agglomerans, while the growth of f. culmorum is i ... | 2000 | 11421000 |
| a novel cryoprotective protein (crp) with high activity from the ice-nucleating bacterium, pantoea agglomerans ifo12686. | the ice-nucleating bacterium, pantoea agglomerans ifo12686, induces the cryoprotective protein (crp) by cold acclimation at 12 degrees c. the crp was purified to apparent homogeneity by various chromatographies. we found that the purified crp was a monomer of approximately 29,000 according to gel filtration chromatography and sds-page, and was a heat-stable protein. the crp could protect freeze-labile enzymes, lactate dehydrogenase (ldh), alcohol dehydrogenase (adh) and isocitrate dehydrogenase ... | 2001 | 11388469 |
| biofilm formation by gram-negative bacteria on central venous catheter connectors: effect of conditioning films in a laboratory model. | human blood components have been shown to enhance biofilm formation by gram-positive bacteria. we investigated the effect of human blood on biofilm formation on the inner lumen of needleless central venous catheter connectors by several gram-negative bacteria, specifically enterobacter cloacae, pseudomonas aeruginosa, and pantoea agglomerans. results suggest that a conditioning film of blood components promotes biofilm formation by these organisms in an in vitro system. | 2001 | 11376074 |
| fhua barrel-cork hybrids are active transporters and receptors. | the crystal structure of escherichia coli fhua reveals a beta-barrel domain that is closed by a globular cork domain. it has been assumed that the proton motive force of the cytoplasmic membrane through the interaction of the tonb protein with the tonb box of the cork opens the fhua channel. yet, deletion of the cork results in an fhua derivative, fhuadelta5-160, that still displays tonb-dependent substrate transport and phage receptor activity. to investigate this unexpected finding further, we ... | 2001 | 11344156 |
| all-d-cecropin b: synthesis, conformation, lipopolysaccharide binding, and antibacterial activity. | cecropin b (lcb) is a natural peptide with antibacterial and antifungal properties. the enantiomer of lcb, containing all-d amino acids (dcb), was synthesized to examine its antibacterial and binding properties. the conformation of dcb was compared to its enantiomer by circular dichroism. both the l- and d-peptides showed an identical induction of alpha-helical secondary structure. however, binding studies between lipopolysaccharide (lps) and dcb or lcb were studied with a dimethylmethylene blue ... | 2001 | 11330824 |
| 16s rdna-based identification of bacteria from conjunctival swabs by pcr and dgge fingerprinting. | establishment of a new molecular biology technique for the identification of multiple bacteria from the ocular environment, which can be applied supplementarily to cultivation in cases of severe bacterial infections. | 2001 | 11328723 |
| variability and interactions between endophytic bacteria and fungi isolated from leaf tissues of citrus rootstocks. | fungi and bacteria were isolated from surface disinfected leaf tissues of several citrus rootstocks. the principal bacterial species isolated were alcaligenes sp., bacillus spp. (including b. cereus, b. lentus, b. megaterium, b. pumilus, and b. subtilis), burkholderia cepacia, curtobacterium flaccumfaciens, enterobacter cloacae, methylobacterium extorquens, and pantoea agglomerans, with p. agglomerans and b. pumilus being the most frequently isolated species. the most abundant fungal species wer ... | 2001 | 11315114 |
| genetic organization of the hrp gene cluster and dspae/bf operon in erwinia herbicola pv. gypsophilae. | erwinia herbicola pv. gypsophilae induces gall formation in gypsophila that is dependent on the existence of a pathogenicity plasmid (ppathehg). we previously demonstrated the presence of several hrp genes on this plasmid. by employing transposon mutagenesis and sequencing, a functional hrp gene cluster on the ppathehg has now been characterized completely. the hrp genes of e. herbicola pv. gypsophilae are remarkably similar to and colinear with those of erwinia amylovora and pantoea stewartii a ... | 2001 | 11277443 |
| cloning and expression of glucose 3-dehydrogenase from halomonas sp. alpha-15 in escherichia coli. | the gene encoding glucose 3-dehydrogenase (g3dh) from halomonas sp. alpha-15 was cloned and expressed in escherichia coli. an open reading frame of 1686 nucleotides was shown to encode g3dh. the flavine adenine dinucleotide binding motif was found in the n-terminal region of g3dh. the deduced primary structure of g3dh showed about 30% identity to sorbitol dehydrogenase from gluconobacter oxydans and 2-keto-d-gluconate dehydrogenases from erwinia herbicola and pantoea citrea. the folding predicti ... | 2001 | 11263965 |
| acyl-homoserine lactone production is more common among plant-associated pseudomonas spp. than among soilborne pseudomonas spp. | a total of 137 soilborne and plant-associated bacterial strains belonging to different pseudomonas species were tested for their ability to synthesize n-acyl-homoserine lactones (nahl). fifty-four strains synthesized nahl. interestingly, nahl production appears to be more common among plant-associated than among soilborne pseudomonas spp. indeed, 40% of the analyzed pseudomonas syringae strains produced nahl which were identified most often as the short-chain nahl, n-hexanoyl-l-homoserine lacton ... | 2001 | 11229911 |
| a cold acclimation protein with refolding activity on frozen denatured enzymes. | we found that a cold acclimation protein from an ice-nucleating bacterium, patoea ananas kuin-3, has refolding activity on frozen denatured protein. based on a sds-page analysis, we confirmed that the cold shock-treated cells of strain kuin-3 could produce some cold acclimation proteins that inhibit their syntheses by the addition of chloramphenicol during the cold acclimation. among such proteins, hsc25 had refolding activity similar to groels. hsc25 was purified to apparent homogeneity by (nh4 ... | 2000 | 11210132 |
| the use of 16s and 16s-23s rdna to easily detect and differentiate common gram-negative orchard epiphytes. | the identification of gram-negative pathogenic and non-pathogenic bacteria commonly isolated from an orchard phylloplane may result in a time consuming and tedious process for the plant pathologist. the paper provides a simple "one-step" protocol that uses the polymerase chain reaction (pcr) to amplify intergenic spacer regions between 16s and 23s genes and a portion of 16s gene in the prokaryotic rrna genetic loci. amplified 16s rdna, and restriction fragment length polymorphisms (rflp) followi ... | 2001 | 11166101 |
| characterization and properties of intracellular proteins after cold acclimation of the ice-nucleating bacterium pantoea agglomerans (erwinia herbicola) ifo12686. | the ice-nucleating bacterium pantoea agglomerans (erwinia herbicola) ifo12686 (ina(+)) responds to a decrease in temperature by the induction of proteins. the pattern of protein bands from strain ifo12686 following a shift in temperature from 30 to 12 degrees c could be divided into four major groups: (1) increasing protein bands, (2) decreasing protein bands, (3) increasing--decreasing protein bands, and (4) almost constant protein bands. we identified a cryoprotective function in the increasin ... | 2000 | 11161552 |
| pantoea agglomerans strain eh318 produces two antibiotics that inhibit erwinia amylovora in vitro. | pantoea agglomerans (synonym: erwinia herbicola) strain eh318 produces through antibiosis a complex zone of inhibited growth in an overlay seeded with erwinia amylovora, the causal agent of fire blight. this zone is caused by two antibiotics, named pantocin a and b. using a genomic library of eh318, two cosmids, pcpp702 and pcpp704, were identified that conferred on escherichia coli the ability to inhibit growth of e. amylovora. the two cosmids conferred different antibiotic activities on e. col ... | 2001 | 11133457 |
| effect of protective agents, rehydration media and initial cell concentration on viability of pantoea agglomerans strain cpa-2 subjected to freeze-drying. | the effect of initial cell density, protective agents and rehydration media on the viability of biocontrol agent pantoea agglomerans cpa-2 when subjected to freeze-drying was studied. several additives were tested as protective agents against freeze-drying injury. maximum viability of the bacterial cells was obtained with disaccharides (survival levels > 60%). freeze-dried samples were rehydrated with several media; the highest percentage viability was obtained with 10% non-fat skim milk (100%+) ... | 2000 | 11119153 |
| impact of fluoroquinolone administration on the emergence of fluoroquinolone-resistant gram-negative bacilli from gastrointestinal flora. | we assessed the risk factors for acquisition of fluoroquinolone-resistant, gram-negative organisms in the gastrointestinal tract of hospitalized patients. we analyzed stool samples from 204 patients and recovered fluoroquinolone-resistant, gram-negative organisms from 63. receipt of fluoroquinolone during the month preceding admission was the only risk factor identified, whereas female sex, duration of hospitalization, exposure to indwelling devices, admission from another hospital, and history ... | 2001 | 11112677 |
| natural incidence of endophytic bacteria in pea cultivars under field conditions. | pea plants grown in the field were used to study the natural incidence of endophytic bacteria in the stem. eleven pea cultivars at the flowering stage were screened for the presence of endophytic bacteria using a printing technique with surface disinfested stem cross-sections on 5% trypticase soy agar (tsa). five cultivars showed colonization. cultivar twiggy showed the highest and most consistent colonization and was further investigated. stems of cv. twiggy at the pod stage were analyzed for e ... | 2000 | 11109492 |
| regulation of hrp genes and type iii protein secretion in erwinia amylovora by hrpx/hrpy, a novel two-component system, and hrps. | two novel regulatory components, hrpx and hrpy, of the hrp system of erwinia amylovora were identified. the hrpxy operon is expressed in rich media, but its transcription is increased threefold by low ph, nutrient, and temperature levels--conditions that mimic the plant apoplast. hrpxy is autoregulated and directs the expression of hrpl; hrpl, in turn, activates transcription of other loci in the hrp gene cluster (z.-m. wei and s. v. beer, j. bacteriol. 177:6201-6210, 1995). the deduced amino -a ... | 2000 | 11059492 |
| enhancement of disease resistance against penaeid acute viraemia and induction of virus-inactivating activity in haemolymph of kuruma shrimp, penaeus japonicus, by oral administration of pantoea agglomerans lipopolysaccharide (lps). | 2000 | 11016589 | |
| 16s ribosomal dna sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. | some bacteria are difficult to identify with phenotypic identification schemes commonly used outside reference laboratories. 16s ribosomal dna (rdna)-based identification of bacteria potentially offers a useful alternative when phenotypic characterization methods fail. however, as yet, the usefulness of 16s rdna sequence analysis in the identification of conventionally unidentifiable isolates has not been evaluated with a large collection of isolates. in this study, we evaluated the utility of 1 ... | 2000 | 11015374 |
| quorum sensing in the plant pathogen erwinia carotovora subsp. carotovora: the role of expr(ecc). | the production of the main virulence determinants of the plant pathogen erwinia carotovora subsp. carotovora, the extracellular cell wall-degrading enzymes, is partly controlled by the diffusible signal molecule n-(3-oxohexanoyl)-l-homoserine lactone (ohhl). ohhl is synthesized by the product of the expi/cari gene. linked to expi we found a gene encoding a putative transcriptional regulator of the luxr-family. this gene, expr(ecc), is transcribed convergently to the expi gene and the two open re ... | 2000 | 10755301 |
| an algorithm for automated bacterial identification using matrix-assisted laser desorption/ionization mass spectrometry. | an algorithm for bacterial identification using matrix-assisted laser desorption/ionization (maldi) mass spectrometry is being developed. this mass spectral fingerprint comparison algorithm is fully automated and statistically based, providing objective analysis of samples to be identified. based on extraction of reference fingerprint ions from test spectra, this approach should lend itself well to real-world applications where samples are likely to be impure. this algorithm is illustrated using ... | 2000 | 10740862 |
| genetic and biochemical characterization of the pathway in pantoea citrea leading to pink disease of pineapple. | pink disease of pineapple, caused by pantoea citrea, is characterized by a dark coloration on fruit slices after autoclaving. this coloration is initiated by the oxidation of glucose to gluconate, which is followed by further oxidation of gluconate to as yet unknown chromogenic compounds. to elucidate the biochemical pathway leading to pink disease, we generated six coloration-defective mutants of p. citrea that were still able to oxidize glucose into gluconate. three mutants were found to be af ... | 2000 | 10735866 |
| biological control of an insect pest by gut-colonizing enterobacter cloacae transformed with ice nucleation gene. | the ice nucleation (in) gene inaa of epiphytic erwinia (pantoea) ananas in10 was transformed into enterobacter cloacae wbmh-3-cmr originated from the faeces of silkworms. the transformant designated as ent. cloacae wbmh-3-cmr(pice6s13) exhibited in activity, unlike the parent strain. the transgenic strain was ingested by mulberry pyralid larvae, fed on detached mulberry leaves, and the supercooling capacity and cold hardiness of these larvae were examined. the mean supercooling point (scp) of th ... | 2000 | 10735247 |
| exploitation of gut bacteria in the locust. | 2000 | 10706273 | |
| dissimilatory metal reduction by the facultative anaerobe pantoea agglomerans sp1. | anaerobic enrichments with acetate as the electron donor and fe(iii) as the terminal electron acceptor were obtained from sediments of salt pond, a coastal marine basin near woods hole, mass. a pure culture of a facultatively anaerobic fe(iii) reducer was isolated, and 16s rrna analysis demonstrated that this organism was most closely related to pantoea (formerly enterobacter) agglomerans, a member of the family enterobacteriaceae within the gamma subdivision of the proteobacteria. this organism ... | 2000 | 10653716 |
| isolation of pantoea agglomerans in two cases of septic monoarthritis after plant thorn and wood sliver injuries. | arthritis after plant injury is often apparently aseptic. we report two cases due to pantoea agglomerans. in one case, the bacterium was isolated only from the pediatric blood culture media, bactec peds plus, monitored in bactec 9240, and not from the other media inoculated with the joint fluid. this procedure could help improve the diagnosis of septic arthritis. | 2000 | 10618144 |
| the phylogenetic position of serratia, buttiauxella and some other genera of the family enterobacteriaceae. | the phylogenetic relationships of the type strains of 38 species from 15 genera of the family enterobacteriaceae were investigated by comparative 16s rdna analysis. several sequences of strains from the genera citrobacter, erwinia, pantoea, proteus, rahnella and serratia, analysed in this study, have been analysed previously. however, as the sequences of this study differ slightly from the published ones, they were included in the analysis. of the 23 enterobacterial genera included in an overvie ... | 1999 | 10555323 |
| distribution of tetracycline resistance genes and transposons among phylloplane bacteria in michigan apple orchards. | the extent and nature of tetracycline resistance in bacterial populations of two apple orchards with no or a limited history of oxytetracycline usage were assessed. tetracycline-resistant (tc(r)) bacteria were mostly gram negative and represented from 0 to 47% of the total bacterial population on blossoms and leaves (versus 26 to 84% for streptomycin-resistant bacteria). a total of 87 isolates were screened for the presence of specific tc(r) determinants. tc(r) was determined to be due to the pr ... | 1999 | 10543801 |
| endotoxins and igg antibodies as indicators of occupational exposure to the microbial contaminants of metal-working fluids. | the aim of this study was to evaluate workers' exposure to microbes and bacterial endotoxins during the use of metal-working fluids (mwf). | 1999 | 10541909 |
| gut colonization by an ice nucleation active bacterium, erwinia (pantoea) ananas reduces the cold hardiness of mulberry pyralid larvae. | to evaluate the suitability of using ice nucleation active (ina) bacteria for the biological control of insect pests, the supercooling point (scp) of larvae of mulberry pyralid, glyphodes duplicalis, and silkworm, bombyx mori, ingesting ina strains of erwinia (pantoea) ananas and pseudomonas syringae was determined. mean scp of the guts of silkworm larvae ingesting ina strains of e. ananas ranged from -2.5 to -2.8 degrees c, being 5 degrees c higher than that in control treatments. similarly, me ... | 1999 | 10413571 |
| gdhb, a gene encoding a second quinoprotein glucose dehydrogenase in pantoea citrea, is required for pink disease of pineapple. | the pink disease of pineapple, caused by the bacterium pantoea citrea, is characterized by a dark coloration on fruit slices after canning. a glucose dehydrogenase (gdh) encoded by the gdha gene has been implicated in the colour formation activity of p. citrea. in this paper it has been shown that p. citrea contains a second, homologous gdh gene and its product, gdhb, represents the main source of gdh activity in this organism. unlike gdha, gdhb is constitutively expressed during the exponential ... | 1999 | 10376838 |
| characterization of plasmids that encode streptomycin-resistance in bacterial epiphytes of apple. | streptomycin resistance in strains of pseudomonas syringae pv. papulans, pantoea agglomerans and a yellow-pigmented, non-fluorescent pseudomonas sp. (py), isolated from apple orchards in new york and washington states, is predominantly associated with stra-strb genes carried on conjugal plasmids (r plasmids). none of 128 resistant erwinia amylovora strains from the eastern and western usa hybridized with a stra-strb probe, smp3. resistant py strains transfered r plasmids to ps. syringae pv. papu ... | 1999 | 10347868 |
| erwinia pyrifoliae sp. nov., a novel pathogen that affects asian pear trees (pyrus pyrifolia nakai) | a novel pathogen from asian pears (pyrus pyrifolia nakai) was analysed by sequencing the 16s rdna and the adjacent intergenic region, and the data were compared to related enterobacteriaceae. the 16s rdna of the asian pear pathogen was almost identical with the sequence of erwinia amylovora, in contrast to the 16s-23s rrna intergenic transcribed spacer region of both species. a dendrogram was deduced from determined sequences of the spacer regions including those of several related species such ... | 1999 | 10319516 |
| reclassification of non-pigmented erwinia herbicola strains from trees as erwinia billingiae sp. nov. | twenty-two erwinia-like strains, isolated from trees since the late fifties and belonging to a distinct phenotypic group with resemblance to pantoea agglomerans, were further characterized by conventional biochemical tests, the biolog metabolic fingerprinting system and fatty acid analysis. their phylogenetic positions were determined by comparing the 16s rrna gene sequence of a representative strain to available sequences of erwinia, pantoea, pectobacterium and brenneria species. the strains we ... | 1999 | 10319458 |
| dust-borne bacteria in animal sheds, schools and children's day care centres. | a total of 316 bacterial strains, including psychrophiles, mesophiles and thermophiles, were isolated and identified from indoor dusts in schools, children's day care centres and animal sheds. several species which had not previously been reported from indoor environments were found: sphingomonas, brevibacterium, nocardiopsis, deinococcus and rhodococcus/gordona. a new psychrophilic actinomycete genus was also found in animal sheds, representing a new undescribed peptidoglycan type and an unusua ... | 1999 | 10212408 |
| new types of antimicrobial compounds produced by lactobacillus plantarum. | new types of antimicrobial compounds were identified in the culture filtrate of lactobacillus plantarum vtt e-78076. activity was detected in the low molecular mass fraction separated by gel chromatography. this fraction totally inhibited the growth of the gram-negative test organism, pantoea agglomerans (enterobacter agglomerans) vtt e-90396. characteristic compounds from this fraction were identified by gc/ms-analysis and the identification was confirmed using pure commercial reference compoun ... | 1999 | 10200070 |
| location and survival of leaf-associated bacteria in relation to pathogenicity and potential for growth within the leaf | the growth and survival of pathogenic and nonpathogenic pseudomonas syringae strains and of the nonpathogenic species pantoea agglomerans, stenotrophomonas maltophilia, and methylobacterium organophilum were compared in the phyllosphere of bean. in general, the plant pathogens survived better than the nonpathogens on leaves under environmental stress. the sizes of the total leaf-associated populations of the pathogenic p. syringae strains were greater than the sizes of the total leaf-associated ... | 1999 | 10103233 |
| identification of an rcsa/rcsb recognition motif in the promoters of exopolysaccharide biosynthetic operons from erwinia amylovora and pantoea stewartii subspecies stewartii. | the regulation of capsule synthesis (rcs) regulatory network is responsible for the induction of exopolysaccharide biosynthesis in many enterobacterial species. we have previously shown that two transcriptional regulators, rcsa and rcsb, do bind as a heterodimer to the promoter of amsg, the first reading frame in the operon for amylovoran biosynthesis in the plant pathogenic bacterium erwinia amylovora. we now identified a 23-base pair fragment from position -555 to -533 upstream of the translat ... | 1999 | 9920870 |
| performance evaluation of disinfectant formulations using poloxamer-hydrogel biofilm-constructs. | poloxamer f127 is a di-block co-polymer of polyoxyethylene and polyoxypropylene. aqueous solutions show thermo-reversible gelation, being liquid at temperatures < 15 degrees c and robust gels at temperatures > 15 degrees c. chilled poloxamer solutions (30% w/v) were inoculated with approximately 10(4-5) cfu ml-1 of stationary phase cultures of pseudomonas aeruginosa, ps. fluorescens, pantoea agglomerans, micrococcus luteus, staphylococcus epidermidis, bacillus subtilis or listeria innocua. drops ... | 1998 | 9871316 |
| allergic alveolitis among agricultural workers in eastern poland: a study of twenty cases. | the aim of this study was to identify the specific agents which caused extrinsic allergic alveolitis (eaa) in the selected group of 20 agricultural workers from eastern poland. the microbiological analysis of the samples of plant materials or dusts reported by the patients as causing symptoms has been carried out, followed by allergological tests (inhalation challenge, agar-gel precipitation test, inhibition of leukocyte migration, skin test) with extrinsic microbial antigens. it was found that ... | 1998 | 9852490 |
| purification and characterization of gallic acid decarboxylase from pantoea agglomerans t71 | oxygen-sensitive gallic acid decarboxylase from pantoea (formerly enterobacter) agglomerans t71 was purified from a cell extract after stabilization by reducing agents. this enzyme has a molecular mass of approximately 320 kda and consists of six identical subunits. it is highly specific for gallic acid. gallic acid decarboxylase is unique among similar decarboxylases in that it requires iron as a cofactor, as shown by plasma emission spectroscopy (which revealed an iron content of 0.8 mol per m ... | 1998 | 9835557 |