Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| complications in hospitalized children with acute gastroenteritis caused by rotavirus: a retrospective analysis. | acute gastroenteritis (age) caused by rotavirus (rv) is a common disease among infants and toddlers, often leading to hospitalization and, in resource-poor countries, to death. however, little is known on specific complications of severe rv-positive (rv+) age and on the clinical course in chronically ill children. this was a retrospective analysis of data for children <5-áyears of age hospitalized due to age during six rotavirus seasons in three large german pediatric hospitals. the primary stud ... | 2011 | 21833497 |
| phylogenetic analysis of vp1, vp2, and vp3 gene segments of genotype g5 group a rotavirus strains circulating in brazil between 1986 and 2005. | genotype g5 group a rotavirus (rv-a), which is common in pigs and also detected in horses and cattle, circulated as endemic genotype in the 1980s and early 1990s in brazil. after 1996, g5 rv-a has been replaced by g9 rv-a, becoming only sporadically detected. recently, g5 has been reported in children with severe diarrhea in argentina, cameroon, paraguay, people's republic of china, and vietnam, suggesting that, although uncommon in humans, it has a worldwide distribution. in a previous study, b ... | 2011 | 21827799 |
| genetic heterogeneity of wild-type g4p[6] porcine rotavirus strains detected in a diarrhea outbreak in a regularly vaccinated pig herd. | group a rotavirus (rv-a) with short electropherotype was identified by ss-page in a neonatal diarrhea outbreak at a brazilian pig farm where the sows were regularly vaccinated with a commercial vaccine containing osu (g5p[7]) and gottfried (g4p[6]) porcine rv-a (porv-a) strains. the ss-page positive stool samples (n=20) were characterized as p[6] genotype by multiplex-nested-rt-pcr assay. the nucleotide analysis of the vp4 gene (vp8*) state that the viruses clustered in p[6] lineages that are al ... | 2011 | 21775076 |
| incidence assessment of rotavirus and adenovirus associated acute gastroenteritis cases in early childhood. | this study was undertaken to give an insight into the incidence of acute gastroenteritis cases of rotaviral/adenoviral aetiology in patients presenting to the emergency room of an inner-city government teaching hospital. group a rotavirus and adenovirus serotype 40-41 antigen results were obtained via immunochromatography. in 2007, there were 1543 patients with gastroenteritis between 0-5 years of age whose stool samples were tested for rota and adenovirus, of whom 386 (25%) had positive stool s ... | 2011 | 21753251 |
| monitoring of group c rotavirus in children with acute gastroenteritis in brazil: an emergent epidemiological issue after rotavirus vaccine? | group c rotavirus (gpcrv) has a worldwide distribution; however, its epidemiology and ecology are still unclear. evidence for a possible zoonotic role has been postulated recently for brazilian children strains. the aim of this study was to monitor gpcrv in children ôëñ15 years with acute gastroenteritis during the 2007-2010 national brazilian rotavirus surveillance, and to undertake the molecular characterization of the major vp6 capsid protein. a total of 3,019 fecal samples were first screene ... | 2011 | 21739455 |
| monitoring the circulation of rotavirus among children after the introduction of the rotarixôäó vaccine in goi+ónia, brazil. | the epidemiological features of rotavirus a (rva) infection differ between children from developing and developed countries which could result in differences in vaccine efficacy around the world. to evaluate the impact of rotarixôäó on rva prevalence, we monitored rva genotypes circulating in goi+ónia by monitoring virus in faecal samples from children that had or had not been previously vaccinated. from february-november of 2008, 220 faecal samples were collected from children in seven day-care ... | 2011 | 21739040 |
| norovirus infections in children under 5 years of age hospitalized due to the acute viral gastroenteritis in northeastern poland. | the primary aim of this study was to evaluate the frequency and seasonality of norovirus infection in hospitalized polish children under 5 years of age, and a secondary aim was to compare the clinical severity of norovirus and rotavirus disease. the prospective surveillance study was carried out from july 2009 through june 2010. stool samples from 242 children hospitalized due to acute viral gastroenteritis were tested for rotavirus group a and adenovirus with commercial immunochromatographic te ... | 2011 | 21732202 |
| grazing management effects on sediment, phosphorus, and pathogen loading of streams in cool-season grass pastures. | erosion and runoff from pastures may lead to degradation of surface water. a 2-yr grazing study was conducted to quantify the effects of grazing management on sediment, phosphorus (p), and pathogen loading of streams in cool-season grass pastures. six adjoining 12.1-ha pastures bisected by a stream in central iowa were divided into three treatments: continuous stocking with unrestricted stream access (csu), continuous stocking with restricted stream access (csr), and rotational stocking (rs). ra ... | 2011 | 21712600 |
| experimental infection of pigs with group a rotavirus and enterotoxigenic escherichia coli in india: gross, histopathological and immunopathological study. | the authors describe a detailed study conducted in assam, india, of gross, histopathological and immunopathological alterations in pigs experimentally infected with rotavirus and enterotoxigenic escherichia coli (etec) expressing k88 pili. a total of 30 caesarean derived piglets were infected experimentally with rotavirus alone or in combination with etec to study the gross and histopathological alterations and the distribution pattern of different b- and t cell subsets in the gut. villus atroph ... | 2011 | 21706463 |
| identification of an antibody binding epitope on the rotavirus non-structural protein nsp2 using phage display analysis. | the nsp2 of rotavirus has important roles in rotavirus replication associated with rna binding, hydrolysis of ntps and rna, and helix destabilising properties. a cell culture assay using a nsp2 specific monoclonal antibody (mab) and polyclonal antiserum to block viral replication showed a 73% and a 96% reduction in the amount of virus produced during replication respectively. phage display technology was used to identify the antibody binding region on the nsp2 protein with the motif (244)t-(y/f) ... | 2011 | 21697352 |
| phylogenetic analysis of rotavirus a nsp2 gene sequences and evidence of intragenic recombination. | the rotavirus non-structural protein nsp2 is one of the earliest and most abundant viral proteins produced during infection. this protein has multiple essential roles in the replication cycle involving rna binding, viroplasm formation, helicase and can hydrolyse the +¦-phosphate of rna and ntps acting as an rtpase and ntpase. in studying sequences from rotavirus strains isolated in australia between 1984 and 2009, the nsp2 gene was seen to be highly conserved and clustered with defined nsp2 geno ... | 2011 | 21689784 |
| genotype diversity of group a rotavirus strains in children with acute diarrhea in urban burkina faso, 2008-2010. | in this study, the diversity of g and p genotypes of rotavirus strains in burkinabe children were examined. between november 2008 and february 2010, 447 stool samples were collected from children <5 years of age with acute diarrhea visiting hospital in ouagadougou. group a rotavirus was previously detected in 151/447 (33.8%) of the samples tested by an immunochromatographic test and these samples were now tested further for rotavirus g and p genotypes by rt-pcr. of these, the rotavirus type gene ... | 2011 | 21678452 |
| high prevalence of enteric viruses in untreated individual drinking water sources and surface water in slovenia. | waterborne infections have been shown to be important in outbreaks of gastroenteritis throughout the world. although improved sanitary conditions are being progressively applied, fecal contaminations remain an emerging problem also in developed countries. the aim of our study was to investigate the prevalence of fecal contaminated water sources in slovenia, including surface waters and groundwater sources throughout the country. in total, 152 water samples were investigated, of which 72 samples ... | 2011 | 21665537 |
| intestinal and extra-intestinal pathogenicity of a bovine reassortant rotavirus in calves and piglets. | despite the impact of bovine group a rotaviruses (garvs) as economically important and zoonotic pathogens, there is a scarcity of data on cross-species pathogenicity and extra-intestinal spread of bovine reassortant garvs. during the course of characterizing the genotypes of all 11 genomic segments of bovine garvs isolated from diarrheic calves in south korea, a unique g6p[7] reassortant garv strain (kj9-1) was isolated. the strain harbors five bovine-like gene segments (vp7: g6; vp6: i2; vp1: r ... | 2011 | 21658866 |
| detection and full genomic analysis of g6p[9] human rotavirus in japan. | a rare genotype g6p[9] was identified in two human group a rotavirus strains designated as kf14 and kf17, that were detected in stool specimens from children with diarrhea in japan. vp7 gene sequences of these two strains were identical and genetically closely related to g6 human rotavirus strains reported in european countries and the united states. to our knowledge, this is the first report of detection of a g6 human rotavirus in japan. for further genetic analysis to elucidate the origin of t ... | 2011 | 21656185 |
| detection of the first g6p[14] human rotavirus strain from a child with diarrhea in egypt. | we report the first detection of a g6p[14] rotavirus strain in egypt from the stool of a child participating in a hospital-based diarrhea surveillance study conducted throughout the year 2004. rotavirus infection was initially detected using a rotavirus group a vp6 enzyme immunoassay; the p (vp4) and g (vp7) genotypes of the strain were identified by rt-pcr. we sequenced the vp7 gene and the vp8* portion of the vp4 gene and the strain displayed the strongest identity to the vp7 [>94% nucleotides ... | 2011 | 21640199 |
| detection of group a rotavirus rna and antigens in serum and cerebrospinal fluid from two children with clinically mild encephalopathy with a reversible splenial lesion. | we report on two children with mild encephalopathy with a reversible splenial lesion associated with group a rotavirus (garv) infection. we examined stool, serum, and cerebrospinal fluid samples to determine the presence of the garv vp7 gene and garv antigen by reverse-transcription pcr and enzyme-linked immunosorbent assay, respectively. garv antigen was detected in stool samples from both patients. the garv g genotype was g9 in one child and g3 in the other. garv antigens were also found in bo ... | 2011 | 21617303 |
| multiple reassortment and interspecies transmission events contribute to the diversity of feline, canine and feline/canine-like human group a rotavirus strains. | rna-rna hybridization assays and complete genome sequence analyses have shown that feline rotavirus (frv) and canine rotavirus (crv) strains display at least two distinct genotype constellations (genogroups), represented by the frv strain rva/cat-tc/aus/cat97/1984/g3p[3] and the human rotavirus (hrv) strain rva/human-tc/jpn/au-1/1982/g3p3[9], respectively. g3p[3] and g3p[9] strains have been detected sporadically in humans. the complete genomes of two crv strains (rva/dog-tc/ita/rv198-95/1995/g3 ... | 2011 | 21609783 |
| uniformity of rotavirus strain nomenclature proposed by the rotavirus classification working group (rcwg). | in april 2008, a nucleotide-sequence-based, complete genome classification system was developed for group a rotaviruses (rvs). this system assigns a specific genotype to each of the 11 genome segments of a particular rv strain according to established nucleotide percent cutoff values. using this approach, the genome of individual rv strains are given the complete descriptor of gx-p[x]-ix-rx-cx-mx-ax-nx-tx-ex-hx. the rotavirus classification working group (rcwg) was formed by scientists in the fi ... | 2011 | 21597953 |
| reassortment among bovine, porcine and human rotavirus strains results in g8p[7] and g6p[7] strains isolated from cattle in south korea. | group a rotaviruses (garvs) cause severe acute gastroenteritis in children and young animals. although zoonotic infections with bovine-like g6 and g8 garvs have been reported in many countries, there is little evidence for reassortment between bovine garvs and garvs from heterologous species. the finding of bovine garvs with the g6 and g8 genotypes in combination with the typical porcine p[7] prompted us to characterize all 11 genes of 30 bovine garvs isolated from clinically infected calves. by ... | 2011 | 21592683 |
| a community outbreak of rotavirus diarrhea associated with exposures in a hospital outpatient department in south china. | introduction:: between october 1 and december 10, 2006, bao'an, china had a 5-fold increase in acute rotavirus gastroenteritis (arge) cases compared with the same time in 2005. to identify the risk factors for arge during this outbreak, we conducted a case-control study among children ≤24 months old from the most heavily affected area. methods:: we defined an arge case as diarrhea, with group a rotavirus antigen detected from fecal samples by the enzyme-linked immunosorbent assay test. in the ca ... | 2011 | 21587095 |
| rotavirus a genotype p[4]g2: genetic diversity and reassortment events among strains circulating in brazil between 2005 and 2009. | group a rotaviruses (rv-a) are the leading cause of severe gastroenteritis in infants and young children worldwide. due to the epidemiologic complexity of rv-a, especially in developing countries, it is important to determine which genotypes are circulating, principally after the introduction in march 2006 of the monovalent (p[8]g1) rotarix® vaccine in brazil by the national immunization program. in phase iii trials with rotarix®, the prevalence of genotype p[4]g2 was extremely low, and therefor ... | 2011 | 21503926 |
| monitoring of group a rotaviruses in wild-living birds in hungary. | rotavirus is a common pathogen causing gastroenteritis in humans and domesticated animals. the incidence of rotavirus in wild-living animals, particularly in avian species, has not been systematically investigated. in this study 1220 fecal samples and cloacal swabs collected from wild-living birds during 2008 in hungary were tested for the presence of group a rotaviruses by a vp6 gene-specific reverse-transcription-polymerase-chain-reaction assay. of the 1220 samples, 276 and 944 were processed ... | 2011 | 21500648 |
| outbreak of rotavirus gastroenteritis in a nursing home, slovenia, december 2010. | a gastroenteritis outbreak affected 45 people (40 residents and five staff) in a nursing home for the elderly in the celje region, north-east slovenia, between 17 december and 31 december 2010. rotavirus group a was laboratory confirmed in the stools of five ill individuals. the outbreak was identified when the number of affected persons was high but was successfully controlled after implementing preventive measures. | 2011 | 21492527 |
| [annual incidence of human group a rotavirus g-serotypes detected in a japanese university hospital between 2003 and 2007]. | group a rotavirus g-serotyping by polymerase chain reaction (pcr) using university hospital subject samples in september 2003 to august 2004, september 2004 to august 2005, september 2005 to august 2006, and september 2006 to august 2007 showed the most common serotypes g1 and g3, detected in 27 and 33 subjects, compared to 4 subjects in whom serotype g4 was detected. between 2003 and 2004, serotypes g1 accounted for 50% and g3 for 38%, contrasting with serotype g3 at 79% between 2004 and 2005, ... | 2011 | 21404601 |
| molecular characterization of group a rotaviruses detected in children with gastroenteritis in ireland in 2006-2009. | summarycommunity and hospital-acquired cases of human rotavirus are responsible for millions of gastroenteritis cases in children worldwide, chiefly in developing countries, and vaccines are now available. during surveillance activity for human rotavirus infections in ireland, between 2006 and 2009, a total of 420 rotavirus strains were collected and analysed. upon either pcr genotyping and sequence analysis, a variety of vp7 (g1-g4 and g9) and vp4 (p[4], p[6], p[8] and p[9]) genotypes were dete ... | 2011 | 21396145 |
| burden and typing of rotavirus group a in latin america and the caribbean: systematic review and meta-analysis. | the efficacy of licensed rotavirus vaccines has only been shown against certain rotavirus group a (rv-a) types. it is critical to understand the burden of rotavirus gastroenteritis (rvge) and its prevalent types to assess the potential impact of these vaccines in latin america and the caribbean (la&c). we performed a systematic review and meta-analyses of all the available evidence reported from 1990 to 2009 on the burden of rotavirus disease and strains circulating in la&c. eligible studies-185 ... | 2011 | 21384462 |
| a single-tube multiplex pcr for rapid detection in feces of 10 viruses causing diarrhea. | a novel multiplex polymerase chain reaction assay was developed to identify 10 viruses in a single tube. the assay was targeted to detect group a and c rotaviruses, adenovirus, norovirus gi, norovirus gii, sapovirus, astrovirus, aichi virus, parechovirus, and enterovirus. a total of 235 stool samples were collected from infants and children with acute gastroenteritis in kyoto, japan, from 2008 to 2009, then tested by this novel multiplex pcr and compared with a multiplex pcr described previously ... | 2011 | 21349292 |
| a waterborne outbreak of epidemic diarrhea due to group a rotavirus in malatya, turkey. | we characterized an outbreak of acute diarrheal disease caused by group a rotavirus that occurred during the autumn of 2005 in malatya city, turkey. a total 9907 patients between 0 to 91 years old (mean age: 25.05?19.67) were included in the epidemic. the patients? data were prospectively collected and statistically analyzed. microbiologic analyses were performed to determine the etiologic agent. rapid onset diarrhea (98.36%), abdominal cramps (69%), fever (44.4%) and vomiting (69.6%) were the m ... | 2011 | 21344142 |
| epidemiology of group a rotaviruses surveillance and burden of disease studies. | human infection has been reported with groups a, b, and c rotaviruses (rvs). of these, group a rvs are the most important, being a major cause of severe gastroenteritis (ge). each year, group a rvs are estimated to cause approx 870,000 deaths worldwide in children less than 5 years (yr) of age, mostly in developing countries (1). this chapter will describe the epidemiological features of group a rv infections, and will critically review the current surveillance strategies used to define the burd ... | 2000 | 21318862 |
| highly sensitive detection of the group a rotavirus using apolipoprotein h-coated elisa plates compared to quantitative real-time pcr. | the principle of a capture elisa is binding of specific capture antibodies (polyclonal or monoclonal) to the surface of a suitable 96 well plate. these immobilized antibodies are capable of specifically binding a virus present in a clinical sample. subsequently, the captured virus is detected using a specific detection antibody. the drawback of this method is that a capture elisa can only function for a single virus captured by the primary antibody. human apolipoprotein h (apoh) or ß2-glycoprote ... | 2011 | 21310042 |
| development of a combined immunomagnetic separation and quantitative reverse transcription-pcr assay for sensitive detection of infectious rotavirus in water samples. | a quantitative and rapid detection method for rotavirus in water samples was developed using immunomagnetic separation combined with quantitative reverse transcription-polymerase chain reaction (ims-rt-qpcr). magnetic beads coated with antibodies against representative group a rotavirus were used to capture and purify intact rotavirus particles in both artificial and real environmental water sample matrix. compared to extracting rna using commercial kits and rt-qpcr assay, the developed ims-rt-q ... | 2011 | 21256895 |
| analysis of human p[4]g2 rotavirus strains isolated in brazil reveals codon usage bias and strong compositional constraints. | the rotavirus genus belongs to the family reoviridae and its genome consist of 11 segments of double-stranded rna. group a rotaviruses (rv-a) are the main etiological agent of acute viral gastroenteritis in infants and young children worldwide. understanding the extent and causes of biases in codon usage is essential to the understanding of viral evolution. however, the factors shaping synonymous codon usage bias and nucleotide composition in human rv-a are currently unknown. in order to gain in ... | 2011 | 21255687 |
| instability of human rotavirus g genotypes circulating in a rural area of bangladesh. | a total of 280 fecal specimens from patients with acute gastroenteritis attending one rural hospital (dharmapasha health complex, sunamgonj) in bangladesh from august 2004 to may 2006 were tested for rotavirus by polyacrylamide gel electrophoresis (page). the diversity of rotavirus was investigated using electropherotyping and reverse transcription-pcr amplification of the vp7 and vp4 genes. the electrophoretic patterns of dsrna of rotavirus showed 9 different migrations (6 long and 3 short) by ... | 2011 | 21240155 |
| rotavirus a genotype g1p[8]: a novel method to distinguish wild-type strains from the rotarix vaccine strain. | rotaviruses are important enteric pathogens for humans and animals. group a rotaviruses (rv-a) are the most common agents of severe gastroenteritis in infants and young children and vaccination is the most effective method to reduce rv-a-associated diseases. g1p[8], the most prevalent rv-a genotype worldwide, is included in the rv-a vaccine rotarix®. the discrimination between wild-type g1p[8] and vaccine g1p[8] strains is an important topic in the study of rv-a epidemiology to manage outbreaks ... | 2010 | 21225209 |
| novel group a rotavirus g8 p[1] as primary cause of an ovine diarrheic syndrome outbreak in weaned lambs. | rotavirus is a worldwide major cause of diarrhea outbreaks in neonatal ruminants. an outbreak of ovine diarrheic syndrome (ods) in 50-75 days-old lambs (weaned lambs) is described. fecal immunochromatography and intestinal immunohistochemistry for rotavirus group a were performed. in addition, semi-nested multiplex rt-pcr for g and p rotavirus genotyping in combination with sequencing were performed, to support the diagnosis and identify the viral strain. a novel ovine rotavirus group a g8 p[1] ... | 2010 | 21216110 |
| human rotavirus genotypes detection among hospitalized children, a study in tehran, iran. | human rotavirus genotypes g1-g4, g9, p[4] and p[8] are major worldwide causes of acute gastroenteritis in children. rotavirus genotype g1p[8] is predominant in many countries. in this study, the genotypic diversity of group a rotaviruses were detected in children <5 years of age who were treated for dehydration and diarrhea in tehran, iran from october 2004 to september 2008. | 2011 | 21194260 |
| increased sensitivity for various rotavirus genotypes in stool specimens by amending three mismatched nucleotides in the forward primer of a real-time rt-pcr assay. | the real-time taqman rt-pcr assay (pang et al., 2004) did not detect 14 clinical samples with rotavirus g2 genotype. three to five nucleotides (nt) were found to be mismatched between the published forward primer when compared to g2p[4], g2p[8], g3p[4], g9p[4], g8 and g12 sequences. an additional forward primer was designed and included in a modified assay to test the 14 clinical samples and 12 samples with known rotavirus g and p genotypes. the modified assay has improved significantly the sens ... | 2010 | 21185331 |
| full genomic analysis of a g8p[1] rotavirus strain isolated from an asymptomatic infant in kenya provides evidence for an artiodactyl-to-human interspecies transmission event. | group a rotavirus (gar) g8p[1] strains, found sometimes in cattle, have been reported rarely from humans. therefore, analysis of the full genomes of human g8p[1] strains are of significance in the context of studies on interspecies transmission of rotaviruses. however, to date, only partial-length nucleotide sequences are available for the 11 genes of a single human g8p[1] strain, while the partial sequences of two other strains have been reported. the present study reports the first complete ge ... | 2011 | 21181935 |
| vp7 gene of human rotavirus a genotype g5: phylogenetic analysis reveals the existence of three different lineages worldwide. | group a rotavirus (rv-a) genotype g5, which is common in pigs, was also detected in children with severe diarrhea in brazil, argentina, paraguay, cameroon, china, thailand, and vietnam. to evaluate the evolutionary relationship among rv-a g5 strains, the vp7 and vp4 genes of 28 brazilian rv-a g5 human strains, sampled between 1986 and 2005, were sequenced and compared with other rv-a g5 strains currently circulating worldwide in animals and humans. the phylogenetic analysis of rv-a g5 vp7 gene s ... | 2011 | 21181934 |
| porcine kobuvirus from pig stool in korea. | to survey for porcine kobuvirus infection, a total of 119 pig fecal samples in korea were collected from three pig farms with good breeding facilities in three provinces. forty-three (36.1%) of the fecal samples tested were positive for porcine kobuvirus. in addition, porcine epidemic diarrhea virus (pedv) and transmissible gastroenteritis virus (tgev) infections were not identified in any of the fecal samples, but porcine group a rotavirus (gar) infection was identified in 11.8% (14/119) of the ... | 2010 | 21161358 |
| genome sequence based molecular epidemiology of unusual us rotavirus a g9 strains isolated from omaha, usa between 1997 and 2000. | after discovery in the early 1980s, rotavirus a serotype g9 was detected infrequently for almost a decade. since the mid-1990s, however, serotype g9 has emerged to become a globally common strain linked to the introduction of a single, new genetic variant of g9 vp7 gene. studies have demonstrated that genetically divergent g9 strains co-circulated at low frequency with the emerging variants. examples include unique u.s. g9 strains om46/hu/usa/1998 and om67/hu/usa/1998, isolated in omaha during t ... | 2010 | 21130184 |
| epidemiology and clinical features of gastroenteritis in hospitalised children: prospective survey during a 2-year period in a parisian hospital, france. | rotavirus is recognised as the most important agent of severe acute gastroenteritis (age) in young children. in a 2-year prospective survey, we investigated the epidemiology and clinical features of the viral and bacterial pathogens in children hospitalised for age. the study was performed in a parisian teaching hospital from november 2001 to may 2004. clinical data were prospectively collected to assess the gastroenteritis severity (20-point vesikari severity score, the need for intravenous reh ... | 2010 | 21128089 |
| reassortant group a rotavirus from straw-colored fruit bat (eidolon helvum). | bats are known reservoirs of viral zoonoses. we report genetic characterization of a bat rotavirus (bat/ke4852/07) detected in the feces of a straw-colored fruit bat (eidolon helvum). six bat rotavirus genes (viral protein [vp] 2, vp6, vp7, nonstructural protein [nsp] 2, nsp3, and nsp5) shared ancestry with other mammalian rotaviruses but were distantly related. the vp4 gene was nearly identical to that of human p[6] rotavirus strains, and the nsp4 gene was closely related to those of previously ... | 2010 | 21122212 |
| frequency, clinical characteristics, and genotype distribution of rotavirus gastroenteritis in greece (2007-2008). | rotavirus is the leading cause of acute gastroenteritis among young children worldwide. a prospective multi-center study was conducted (2007-2008) in five pediatric hospitals to determine the prevalence, the clinical characteristics, and genotype distribution of rotavirus infection in greece. faecal samples were examined for the presence of group a rotavirus antigen by immunochromatography. rotavirus strains were subjected to g and p genotyping by reverse-transcriptase polymerase chain reaction ... | 2011 | 21108355 |
| immunogenicity and virus-like particle formation of rotavirus capsid proteins produced in transgenic plants. | the human pathogen, group a rotavirus, is the most prevalent cause of acute infantile and pediatric gastroenteritis worldwide, especially in developing countries. there is an urgent demand for safer, more effective and cheaper vaccines against rotavirus infection. plant-derived antigens may provide an exclusive way to produce economical subunit vaccines. virus-like particles, constituting viral capsid proteins without viral nucleic acids, are considered a far safer candidate compared with live a ... | 2010 | 21104033 |
| [rotavirus prevalence in infants and children in the public healthcare system of the state of pernambuco]. | rotaviruses are considered important etiological agents of acute gastroenteritis and a common cause of the hospitalization of children aged zero to four years-old. in brazil, the incidence of rotavirus gastroenteritis in children is 12 to 42% and the distribution of infection is related to seasonality, which apparently occurs in different periods and intensity according to each region. the study investigated group a rotavirus in fecal samples of suspected children attended by the public health s ... | 2010 | 21085867 |
| predominance of porcine p[23] genotype rotaviruses in piglets with diarrhea in northern thailand. | of 131 stool samples collected from piglets with diarrhea in northern thailand between july 2006 and august 2008, 14 (10.7%) were positive for group a rotavirus. sequence analysis showed that 13 strains (92.9%) belonged to the rare p[23] genotype combination with g9 or g3 genotypes. | 2010 | 21084504 |
| a hospital based study on inter- and intragenotypic diversity of human rotavirus a vp4 and vp7 gene segments, germany. | efforts to reduce the impact of group a rotaviruses on human morbidity and mortality rely on oral immunisation with live attenuated or recombinant vaccines. a major challenge in immunisation is the vast inter- and intragenotypic diversity accomplished by circulating rotaviruses. | 2010 | 21074491 |
| [epidemiological surveillance of norovirus and rotavirus diarrhea among outpatient children in five metropolitan cities]. | to survey the clinical epidemiological features of norovirus and rotavirus diarrhea among children living in 5 cities. | 2010 | 21055297 |
| laboratory-based rotavirus surveillance during the introduction of a vaccination program, brazil, 2005-2009. | : brazil introduced universal antirotavirus vaccination in march 2006. this article reports the results of rotavirus a (rv-a) surveillance from january 2005 to december 2009. | 2011 | 21048523 |
| [detection of bovine rotavirus g10p[11] in a diary farm in daqing, china]. | group a rotavirus are the most frequently detected viral agent associated with the acute diarrhea in calves. in order to investigate the situation of rotavirus strains circulating in diary farms, a total of 117 fecal specimens were collected from diarrhea calves under 4 weeks-age on yinluo diary farm in daqing region in china from 2008 to 2009. ten specimens were detected to be positive by a rotavirus group a diagnostic kit, which confirmed that the rotavirus was important viral agent associated ... | 2010 | 21043143 |
| quantification and molecular characterization of enteric viruses detected in effluents from two hospital wastewater treatment plants. | hospital wastewater has been described as an important source of spreading pathogenic microorganisms in the environment. however, there are few studies reporting the presence and concentrations of gastroenteric viruses and hepatitis a viruses in these environmental matrices. the aim of this study was to assess the contamination by viruses responsible for acute gastroenteritis and hepatitis derived from hospital wastewater treatment plants (wwtps). rotavirus a (rv-a), human adenoviruses (hadv), n ... | 2010 | 21040941 |
| full genomic analysis of a simian sa11-like g3p[2] rotavirus strain isolated from an asymptomatic infant: identification of novel vp1, vp6 and nsp4 genotypes. | we report here the full genomic analysis of a simian sa11-like g3p[2] group a rotavirus (gar) strain, b10, isolated from an asymptomatic infant in kenya in 1987. by nucleotide sequence identities and phylogenetic analyses, the vp7-vp4-vp2-vp3-nsp1-nsp2-nsp3-nsp5 genes of strain b10 exhibited maximum genetic relatedness to those of the different isolates of simian strain sa11, and were assigned to the g3-p[2]-c5-m5-a5-n5-t5-h5 genotypes, respectively. on the other hand, the vp1, vp6 and nsp4 gene ... | 2010 | 21035567 |
| comparison of the rapid methods for screening of group a rotavirus in stool samples. | in this study, the sensitivity and specificity of three immunochromatography (ic) test kits for rapid detection of group a rotavirus were compared and evaluated with stool samples collected from children, who suffered from acute gastroenteritis during february to june, 2009 in japan. a total of 86 stool samples were tested and compared with a reference rt-pcr method. the sensitivity among ip-rota v, dipstick eiken rota and rota-adeno test kits were 97.2, 95.8 and 88.7%, while the specificity wer ... | 2010 | 21030457 |
| molecular characterization of unusual bovine group a rotavirus g8p[14] strains identified in western india: emergence of p[14] genotype. | a total of 78 fecal specimens were collected from both apparently healthy (n=71) and diarrheic (n=7) cattle from an organized farm in pune, western india in december 2007-january 2008. three specimens tested positive for group a rotavirus (rv) by antigen capture elisa were subjected to rt-pcr for amplification of entire coding regions of three structural (vp4, vp6 and vp7) and one nonstructural (nsp4) genes. all three strains were genotyped as g8p[14]. phylogenetic analysis of the vp7 and vp4 ge ... | 2010 | 20880637 |
| determination of g and p type diversity of group a rotaviruses in faecal samples of diarrhoeic calves in kashmir, india. | to determine the genetic diversity of group a rotaviruses in bovine calves in kashmir, india. | 2010 | 20875036 |
| [study on the epidemiological of rotavirus diarrhea in lulong in 2008-2009]. | to analyze the feature of epidemiological of rotavirus diarrhea in lulong county, hebei province. | 2010 | 20848835 |
| outbreak of acute gastroenteritis in young children with death due to rotavirus genotype g9 in rio branco, brazilian amazon region, 2005. | an epidemic of acute gastroenteritis occurred in rio branco city, acre state, in brazil's amazon region in 2005. an investigation was conducted to confirm the etiology and identify possible risk factors for death. | 2010 | 20843719 |
| diagnosis of viral gastroenteritis by simultaneous detection of adenovirus group f, astrovirus, rotavirus group a, norovirus genogroups i and ii, and sapovirus in two internally controlled multiplex real-time pcr assays. | norovirus, rotavirus group a, astrovirus, sapovirus and adenovirus serotypes 40 and 41, are common causes of gastroenteritis. conventional diagnosis of these causative agents is based on antigen detection and electron microscopy. | 2010 | 20829103 |
| multiple-gene characterization of rotavirus strains: evidence of genetic linkage among the vp7-, vp4-, vp6-, and nsp4-encoding genes. | a total of 162 rotavirus strains detected between 1996 and 2006 among individuals with diarrhea in rio de janeiro, brazil, were analyzed by multiple-gene genotyping. characterization of strains was done by rt-pcr assay for amplification and typing of the vp7-, vp4-, vp6-, and nsp4-encoding genes. overall, 139 (85.8%) strains belonged to the common group a rotavirus combinations: 67 (41.4%) belonged to genotype g1-p[8]-i1-e1; 18 (11.1%) were g2-p[4]-i2-e2; 11 (6.8%) were g3-p[8]-i1-e1; 12 (7.4%) ... | 2010 | 20827779 |
| detection of rotavirus a in sewage samples using multiplex qpcr and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration. | group a rotaviruses (rv-a) are the most common agents of viral gastroenteritis in children worldwide. the goal of this study was to compare two different methods to concentrate rv-a from sewage samples and to improve the detection and quantification of rv-a using a multiplex quantitative pcr assay with an internal control. both rv-a and the internal control virus, bacteriophage pp7, were seeded into wastewater and then concentrated using either an ultrafiltration-based adsorption-elution protoco ... | 2010 | 20804786 |
| multiplex semi-nested rt-pcr with exogenous internal control for simultaneous detection of bovine coronavirus and group a rotavirus. | neonatal calf diarrhea is a multi-etiology syndrome of cattle and direct detection of the two major agents of the syndrome, group a rotavirus and bovine coronavirus (bcov) is hampered by their fastidious growth in cell culture. this study aimed at developing a multiplex semi-nested rt-pcr for simultaneous detection of bcov (n gene) and group a rotavirus (vp1 gene) with the addition of an internal control (mrna nd5). the assay was tested in 75 bovine feces samples tested previously for rotavirus ... | 2010 | 20723564 |
| assessment of adenovirus, hepatitis a virus and rotavirus presence in environmental samples in florianopolis, south brazil. | to assess the presence of human adenovirus (hadv), hepatitis a (hav) virus and rotavirus a (rv-a) in environmental samples from the southern region of brazil and to provide viral contamination data for further epidemiological studies and governmental actions. | 2010 | 20698910 |
| rotavirus strain diversity in the centre coast of tunisia from 2000 through 2003. | an epidemiological survey investigating rotavirus infection in children was undertaken in the coastal region of tunisia from january 2000 through september 2003. a total of 309 fecal specimens were screened by enzyme-linked immunosorbent assay and latex agglutination assay for the presence of group a rotavirus antigen. the detection rate was 26.2%. rotavirus outbreaks showed a temperature-dependant pattern (p = .026) but no significant association with rainfall. rotavirus strains isolated were a ... | 2010 | 20684712 |
| molecular characterization and genotyping of human rotavirus strains in abidjan, cote d'ivoire. | in this study, we characterized human rotavirus strains recovered from infants and young children with acute diarrhea in abidjan, cote d'ivoire, during 2000-2004. in total, 719 fecal specimens were collected from children aged 1-60 months with acute infantile gastroenteritis. examination with a commercial enzyme-linked immunosorbent assay showed the presence of group a rotavirus antigen in 208 diarrheal specimens (28.9%). polyacrylamide gel electrophoresis of the rna extracted from rotavirus-pos ... | 2010 | 20684706 |
| characterization of human rotavirus recovered from children with acute diarrhea in kinshasa, democratic republic of congo. | between july and october of 2003, 2004, and 2005, outbreaks of acute gastroenteritis occurred among children <5 years of age in kinshasa, democratic republic of the congo. stool specimens (67 in 2003, 108 in 2004, and 116 in 2005) were collected and screened for rotaviruses using either latex agglutination (diarlex laa; orion diagnostics) or enzyme immunoassay (ideia; dakocytomation). the molecular characteristics of the rotavirus strains were then determined. group a rotavirus was detected in 1 ... | 2010 | 20684702 |
| molecular characterization of rotavirus strains circulating among children with acute gastroenteritis in madagascar during 2004-2005. | a survey was undertaken of the etiology of acute gastroenteritis in children <16 years of age in antananarivo, madagascar, from may 2004 through may 2005. with use of electron microscopy of fecal specimens, 104 (36%) of 285 children were found to be infected with rotavirus. rotavirus strain characterization was undertaken using enzyme-linked immunosorbent assay, electropherotyping, reverse-transcription polymerase chain reaction genotyping, and nucleotide sequencing. the predominant group a rota ... | 2010 | 20684699 |
| epidemiology of rotavirus infection in children in blantyre, malawi, 1997-2007. | acute gastroenteritis caused by rotavirus infection is an important cause of morbidity and mortality among infants and young children in africa. from 1997 through 2007, we enrolled 3740 children <5 years of age with acute gastroenteritis who received hospital care at the queen elizabeth central hospital in blantyre, malawi. group a rotavirus was detected in fecal specimens by enzyme immunoassay. rotavirus strains were characterized for vp7 (g) and vp4 (p) types with use of reverse-transcription ... | 2010 | 20684698 |
| prevalence and diversity of rotavirus strains in children with acute diarrhea from rural communities in the limpopo province, south africa, from 1998 to 2000. | data regarding the prevalence and molecular epidemiology of rotavirus infection in rural areas of africa are limited. in this study the prevalence and genetic diversity of rotaviruses in a rural south african setting were investigated. | 2010 | 20684695 |
| molecular analysis of human group a rotavirus g10p[14] genotype in slovenia. | rotavirus g10 genotype is one of the main rotaviruses circulating in cattle throughout the world but is also found in asymptomatic and symptomatic infections in children, and thought to be acquired through zoonotic transmission. | 2010 | 20675186 |
| genomic analysis of codon, sequence and structural conservation with selective biochemical-structure mapping reveals highly conserved and dynamic structures in rotavirus rnas with potential cis-acting functions. | rotaviruses are a major cause of acute, often fatal, gastroenteritis in infants and young children world-wide. virions contain an 11 segment double-stranded rna genome. little is known about the cis-acting sequences and structural elements of the viral rnas. using a database of 1621 full-length sequences of mammalian group a rotavirus rna segments, we evaluated the codon, sequence and rna structural conservation of the complete genome. codon conservation regions were found in eight orfs, suggest ... | 2010 | 20671030 |
| identification of a g2-like porcine rotavirus bearing a novel vp4 type, p[32]. | a porcine group a rotavirus (garv) strain, 61/07/ire, was isolated from a 4-5 week asymptomatic piglet, during an epidemiological survey of porcine herds in southern ireland, in 2007. the nucleotide (nt) and amino acid (aa) sequence of the full-length vp4 protein of the porv strain 61/07/ire was determined. based on the entire vp4 open reading frame (nt), strain 61/07/ire displayed ≤76.5% identity to representatives of the established 31 p-types, a value far lower than the percentage identity cu ... | 2010 | 20663474 |
| [expression and assembly of rotavirus-like particles in insect cells mediated by recombinant bombyx mori multibac]. | to construct recombinant baculoviruses co-expressing three structural genes vp2, vp6 and vp7 of rotavirus, and assemble rotavirus-like particles (vlps) in bmn cells. | 2010 | 20650749 |
| the genome segments of a group d rotavirus possess group a-like conserved termini but encode group-specific proteins. | rotaviruses are a leading cause of viral acute gastroenteritis in humans and animals. they are grouped according to gene composition and antigenicity of vp6. whereas group a, b, and c rotaviruses are found in humans and animals, group d rotaviruses have been exclusively detected in birds. despite their broad distribution among chickens, no nucleotide sequence data exist so far. here, the first complete genome sequence of a group d rotavirus (strain 05v0049) is presented, which was amplified usin ... | 2010 | 20631147 |
| chemical and microbiological parameters as possible indicators for human enteric viruses in surface water. | there are still conflicting results on the suitability of chemical and microbiological parameters as indicators for the viral contamination of surface waters. in this study, conducted over 20 months, the abundance of human adenovirus, human polyomavirus, enterovirus, group a rotavirus and norovirus was determined in ruhr and rhine rivers, germany. additionally, prevalence of different possible indicators such as somatic coliphages, e. coli, intestinal enterococci, and total coliforms was also co ... | 2010 | 20627679 |
| comparative efficacy of immunological, molecular and culture assays for detection of group a rotavirus from faecal samples of buffalo (bubalus bubalis) calves. | group a rotaviruses play an important role in causing gastroenteritis and mortality in buffalo (bubalus bubalis) calves. a number of assays like rna-polyacrylamide gel electrophoresis (rna-page), enzyme-linked immunosorbent assay (elisa), reverse transcription polymerase chain reaction (rt-pcr) and virus isolation have been employed for rotavirus diagnosis. we evaluated the comparative efficacy of different assays for detection of group a rotavirus in buffalo calves. a total of 455 faecal sample ... | 2010 | 20607400 |
| molecular characterization of rotaviruses in a japanese raccoon dog (nyctereutes procyonoides) and a masked palm civet (paguma larvata) in japan. | group a rotaviruses infect and cause diarrhea in humans and a wide range of mammals. previous studies have suggested that some strains can cross the species barrier to infect humans (martella et al., 2010). however, there are few reports on infection and characterization of rotaviruses in wild animals. to estimate what types of rotaviruses infect wild animals, we investigated infection of rotaviruses in wild animals living in urban areas in japan between 2003 and 2008. of 145 fecal specimens obt ... | 2010 | 20605380 |
| molecular characterization of human group a rotavirus from stool samples in young children with diarrhea in indonesia. | detection and genotyping of group a rotavirus strains from stool samples in young children with diarrhea in indonesia were examined using reverse transcription-nested multiplex pcr. of 421 stool specimens, 257 samples was rotavirus positive. g1 type was the most common g-type (54%), followed by g2 (6%) and g9 (3%). p[8] was the most common p-type (39%), followed by p[6] (19%), p[4] (10%) and p[11] 1%. eighteen percent of the samples had mixed g genotype infection and 5% had mixed p genotype infe ... | 2010 | 20578517 |
| concentration of acrylamide in a polyacrylamide gel affects vp4 gene coding assignment of group a equine rotavirus strains with p[12] specificity. | it is universally acknowledged that genome segment 4 of group a rotavirus, the major etiologic agent of severe diarrhea in infants and neonatal farm animals, encodes outer capsid neutralization and protective antigen vp4. | 2010 | 20573245 |
| chemical and microbiological parameters as possible indicators for human enteric viruses in surface water. | there are still conflicting results on the suitability of chemical and microbiological parameters as indicators for the viral contamination of surface waters. in this study, conducted over 20 months, the abundance of human adenovirus, human polyomavirus, enterovirus, group a rotavirus and norovirus was determined in ruhr and rhine rivers, germany. additionally, prevalence of different possible indicators such as somatic coliphages, e. coli, intestinal enterococci, and total coliforms was also co ... | 2010 | 20556879 |
| detection and molecular characterization of enteric viruses in environmental samples in monastir, tunisia between january 2003 and april 2007. | a prospective study was performed to characterize the main human enteric viruses able to persist in sewage samples and in shellfish tissues, and to establish the correlation between environmental strains and viral infantile diarrhoea observed in the same area during the same period. | 2010 | 20553345 |
| changing profile of the bovine rotavirus g6 population in the south of ireland from 2002 to 2009. | bovine group a rotavirus is one of the main causes of neonatal diarrhoea in calves. this study examined the different g and p genotypes circulating in the bovine population, from 2002-2009, in the south of ireland. rotavirus positive bovine faecal samples (n=332) were collected from the cork regional veterinary laboratory, between 2002 and 2009 and subjected to rna extraction, page analysis, and g and p genotyping. genotyping analysis identified g6, g10, p[5], and p[11] to be the predominant g a ... | 2010 | 20541335 |
| molecular characterization and epidemiology of rotavirus isolates obtained from children with diarrhoea in malaysia. | this retrospective study examined the g/p type of rotavirus in rna samples that have previously been e-typed by rna-page in 1996. the results were then compared to 2007 samples to ascertain the extent of changes that may have occurred in this 11-years time interval. the g and p genotypes were determined by hemi-nested pcr and further analysed by phylogenetic study. in 1996, the g/p combination g1p[8], g(ut)p[8] and g1p(ut) prevalence rate were 81%, 9% and 7%, respectively. as expected, the g9 ge ... | 2009 | 20527266 |
| detection and characterisation of group a rotavirus in asymptomatic piglets in southern ireland. | porcine group a rotaviruses (garv) are causative agents of enteritis in piglets and are a large reservoir of genetic material for the diversification of human garvs. accumulation of information on the genetic heterogeneity of porcine viruses is pivotal for readily characterising unusual human strains. screening of 292 fecal samples, collected from 4-5- to 8-9-week-old asymptomatic pigs from four herds in ireland between 2005 and 2007 resulted in 19 (6.5%) samples testing positive by reverse-tran ... | 2010 | 20526785 |
| correlation between rotavirus a genotypes detected in hospitalized children and sewage samples in 2006, córdoba, argentina. | routine rotavirus a (rv-a) surveillance is based on clinical cases, so only symptomatic infections are reported. the objective of this study was to determine whether the rv-a genotypes and cold seasonal pattern described in patients with diarrhea is reflected by sewage surveillance, which could be representative of the rv-a genotypes circulating in the population. the genotype distribution of rv-a in effluent samples from a local sewage treatment plant was compared to those from local clinical c ... | 2010 | 20513096 |
| detection of uncommon rotavirus a strains p[8]g8 and p[4]g8 in the city of rio de janeiro, 2002. | group a rotaviruses (rv-a) are the major cause of gastroenteritis in infants and young children around the world. each year rv-a causes approximately 11 million episodes of severe diarrhea, with an estimated of 611,000 deaths. epidemiologic surveys have identified p[8]g1, p[4]g2, p[8]g3, p[8]g4, and p[8]g9 as the most common global genotypes associated with diarrhea in children up to 5-year old. surveillance studies and documentation of rv-a g and p genotypes is necessary for a comprehensive eva ... | 2010 | 20513095 |
| complete genome constellation of a caprine group a rotavirus strain reveals common evolution with ruminant and human rotavirus strains. | this study reports the first complete genome sequence of a caprine group a rotavirus (gar) strain, go34. the vp7-vp4-vp6-vp1-vp2-vp3-nsp1-nsp2-nsp3-nsp4-nsp5 genes of strain go34, detected in bangladesh, were assigned to the g6-p[1]-i2-r2-c2-m2-a11-n2-t6-e2-h3 genotypes, respectively. strain go34 was closely related to the vp4, vp6-7 and nsp4-5 genes of bovine gars and the nsp1 gene of go34 to an ovine gar. strain go34 shared low nucleotide sequence identities (<90 %) with vp2-3 genes of other g ... | 2010 | 20505013 |
| noroviruses as cause of gastroenteritis in elderly patients. | background and aims. gastroenteritis is one of the infections that are particularly important to the elderly people. the knowledge of the main pathogens causing gastroenteritis in this group of patients, whose number will be dramatically increased in coming decades, is essential. the contribution of group a rotavirus, adenovirus type 41 and 42, norovirus and astrovirus as cause of gastroenteritis among patients of all ages, especially those with more than 65 years old over an extended time perio ... | 2010 | 20460955 |
| rearranged genomic rna segments offer a new approach to the reverse genetics of rotaviruses. | group a rotaviruses (rv), members of the reoviridae family, are a major cause of infantile acute gastroenteritis. the rv genome consists of 11 double-stranded rna segments. in some cases, an rna segment is replaced by a rearranged rna segment, which is derived from its standard counterpart by partial sequence duplication. we report here a reverse genetics system for rv based on the preferential packaging of rearranged rna segments. using this system, wild-type or in vitro-engineered forms of rea ... | 2010 | 20427539 |
| molecular epidemiology of group a rotavirus in buenos aires, argentina 2004-2007: reemergence of g2p[4] and emergence of g9p[8] strains. | detection and characterization of group a rotavirus in buenos aires, argentina, was conducted on 710 fecal samples from children 0-15 years old collected between 2004 and 2007. rotavirus was detected in 140 (19.7%) samples with g9p[8] (30.0%) and g2p[4] (21.4%) as the most common genotypes. mixed (g and/or p) infections accounted for 17.9% of the samples and the emerging g12 strain was detected during 2004 (3.5%) and 2007 (2.5%). genotype g2 was the most prevalent during 2004 (43.9%) and 2007 (5 ... | 2010 | 20419826 |
| genetic characterization of norovirus gii.4 2006b variants from jeju island, south korea. | ninety-seven fecal specimens collected from children with acute gastroenteritis between 2007 and 2008 that were found to be negative for group a rotavirus in prescreening by elisa with vp6-specific antibody were re-screened for viruses by reverse transcription (rt)-pcr. forty (41.2%) samples were found to be positive for virus by rt-pcr; of these, norovirus (32.5%, n = 13) and rotavirus (32.5%, n = 13) were the most common, followed by astrovirus (5.0%, n = 2) and enterovirus (2.5%, n = 1). co-i ... | 2010 | 20419823 |
| detection and genotyping of korean porcine rotaviruses. | porcine group a rotavirus (garv) is considered to be an important animal pathogen due to their economic impact in the swine industry and its potential to cause heterologous infections in humans. this study examined 475 fecal samples from 143 farms located in 6 provinces across south korea. rt-pcr and nested pcr utilizing primer pairs specific for the garv vp6 gene detected garv-positive reactions in 182 (38.3%) diarrheic fecal samples. a total of 98 porcine garv strains isolated from the garv-po ... | 2010 | 20359834 |
| geographical differences in the proportion of human group a rotavirus strains within new zealand during one epidemic season. | the prospect of future rotavirus vaccine programs means it is important to understand rotavirus strain diversity within new zealand, especially if this was to influence vaccine effectiveness. the g-genotype of 359 group a rotavirus strains isolated from 416 stool samples collected from june 2005 to may 2006 (inclusive) from children less than 5 years of age in multiple centers throughout new zealand was determined. g1 was the dominant circulating strain (55.8%) followed by g4 (21.4%), g3 (3.4%), ... | 2010 | 20336723 |
| rotavirus antigenemia and genomia in children with rotavirus gastroenteritis. | we investigated group a rotavirus (garv) antigenemia and genomia in children with rotavirus gastroenteritis. a total of 16 patients (2-29 months old), who received a diagnosis of garv gastroenteritis using a commercial rapid test, were enrolled in this study. the sera from the patients were tested for the presence of garv antigen and the vp7 and nsp3 genes using an enzyme-linked immunosorbent assay (elisa) and reverse transcription-polymerase chain reaction, respectively. furthermore, when the v ... | 2010 | 20332567 |
| genotypic linkages of vp4, vp6, vp7, nsp4, nsp5 genes of rotaviruses circulating among children with acute gastroenteritis in thailand. | rotavirus is the main cause of acute viral gastroenteritis in infants and young children worldwide. surveillance of group a rotavirus has been conducted in chiang mai, thailand since 1987 up to 2004 and those studies revealed that group a rotavirus was responsible for about 20-61% of diarrheal diseases in hospitalized cases. in this study, we reported the continuing surveillance of group a rotavirus in 2005 and found that group a rotavirus was detected in 43 out of 147 (29.3%) stool samples. fiv ... | 2010 | 20223298 |
| novel light-upon-extension real-time pcr assay for simultaneous detection, quantification, and genogrouping of group a rotavirus. | we have developed a light-upon-extension (lux) real-time pcr assay for detection, quantification, and genogrouping of group a rotavirus (rv), the most common cause of acute gastroenteritis in children. the lux system uses a fluorophore attached to one primer and having a self-quenching hairpin structure, making it cost-effective and specific. we designed genogroup-specific primers having different fluorophores, making it possible to differentiate between the two main genogroups of human group a ... | 2010 | 20220154 |
| analysis of genetic diversity and molecular evolution of human group b rotaviruses based on whole genome segments. | group b rotavirus (gbr) is a rare enteric pathogen that causes severe diarrhoea, primarily in adults. nearly full-length sequences of all 11 rna segments were determined for human gbrs detected recently in india (idh-084 in 2007, ic-008 in 2008), bangladesh (bang117 in 2003) and myanmar (mmr-b1 in 2007), and analysed phylogenetically with the sequence data of gbrs reported previously. all rna segments of gbr strains from india, bangladesh and myanmar showed >95 % nucleotide sequence identities. ... | 2010 | 20200192 |
| rotavirus nsp5 orchestrates recruitment of viroplasmic proteins. | rotavirus genome replication and the first steps of virus morphogenesis take place in cytoplasmic viral factories, called viroplasms, containing four structural (vp1, vp2, vp3 and vp6) and two non-structural (nsp2 and nsp5) proteins. nsp2 and nsp5 have been shown to be essential for viroplasm formation and, when co-expressed in uninfected cells, to form viroplasm-like structures (vls). in the present work, vls formation was shown upon co-expression of nsp5 with the core protein vp2 despite the a ... | 2010 | 20200190 |
| rotavirus-associated diarrhoea in foals in greece. | severe outbreaks of diarrhoeic syndrome occurred in young foals at the same stud farm during two consecutive breeding periods namely spring 2006 and 2007. rotavirus-like particles were detected by electron microscopy in the faeces of the affected foals and group a rotavirus infection was confirmed by reverse-transcription (rt)-pcr with selected sets of rotavirus-specific primers. sequence analysis of the genes encoding the outer capsid rotavirus proteins vp7 and vp4 enabled classification of the ... | 2010 | 20197218 |