Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| linking the genomes of nonmodel teleosts through comparative genomics. | recently the genomes of two more teleost species have been released: the medaka (oryzias latipes), and the three-spined stickleback (gasterosteus aculateus). the rapid developments in genomics of fish species paved the way to new and valuable research in comparative genetics and genomics. with the accumulation of information in model species, the genetic and genomic characterization of nonmodel, but economically important species, is now feasible. furthermore, comparison of low coverage gene map ... | 2008 | 18297360 |
| investigating the presence of organochlorine pesticides and polychlorinated biphenyls in wild and farmed gilthead sea bream (sparus aurata) from the western mediterranean sea. | the presence of organochlorine pesticides and polychlorinated biphenyls in wild and farmed gilthead sea bream (sparus aurata) from the western mediterranean, spain, is investigated. these pollutants were determined in white and red muscle and liver by gas chromatography coupled to tandem mass spectrometry. contaminant profiles present in both wild and farmed fish tissues include ddts, pcbs and hcb. the highest concentrations of contaminants were found in liver, and an increase in pollutant level ... | 2008 | 18289607 |
| cellular localization of insulin-like growth factor-ii protein in the sea bass (dicentrarchus labrax) from hatching to adult. | the cellular localization of igf-ii protein was investigated during larval and postlarval developmental stages of sea bass (dicentrarchus labrax) by immunohistochemistry using antisera raised against sparus aurata igf-ii. at hatching, igf-ii immunoreactivity was already present in the skin, developing intestine and skeletal muscle. during larval life igf-ii protein was also observed in heart musculature, in kidney and gill epithelia as well as in liver. in fry skeletal muscle a moderate igf-ii i ... | 2008 | 18283636 |
| differential localization and regulation of two aquaporin-1 homologs in the intestinal epithelia of the marine teleost sparus aurata. | aquaporin (aqp)-mediated intestinal water absorption may play a major osmoregulatory role in euryhaline teleosts, although the molecular identity and anatomical distribution of aqps in the fish gastrointestinal tract is poorly known. here, we have investigated the functional properties and cellular localization in the intestine of two gilthead seabream (sparus aurata) homologs of mammalian aquaporin-1 (aqp1), named saaqp1a and saaqp1b. heterologous expression in xenopus laevis oocytes showed tha ... | 2008 | 18171690 |
| molecular cloning and characterization of follistatin in the gilthead sea bream, sparus aurata. | follistatin (fst) is an activin-binding protein that neutralizes the activity of activin. fst also binds other members of the transforming growth factor-beta (tgf-beta) superfamily, including myostatin (mstn). we report herein on the isolation and characterization of a full-length cdna sequence predicted to encode fst in a marine fish, the gilthead sea bream sparus aurata. the deduced amino acid sequence of sea bream fst (safst) is highly conserved to the counterpart sequences in other vertebrat ... | 2009 | 18167029 |
| confinement exposure induces glucose regulated protein 75 (grp75/mortalin/mthsp70/pbp74/hspa9b) in the hepatic tissue of gilthead sea bream (sparus aurata l.). | glucose regulated protein 75 (grp75/mortalin/mthsp70/pbp74/hspa9b) is a molecular chaperone that was partially cloned and sequenced in gilthead sea bream (sparus aurata l.) using a rt-pcr and 3'race approach. the deduced amino acid sequence supported the early vertebrate divergence of the heat shock protein 70 family into cytoplasmic hsp70/hsc70 group, endoplasmic reticulum-resident group and the mitochondrial-type group of grp75. the tissue-specific regulation of grp75 was analyzed by real-time ... | 2008 | 18164226 |
| total substitution of fish oil by vegetable oils in gilthead sea bream (sparus aurata) diets: effects on hepatic mx expression and some immune parameters. | the use of vegetable oils in fish nutrition has been extensively studied; and recent work has focused attention on replacing fish oil with alternative fatty acid sources and their effect on the immune system. however, little is known about the effect of these oils on immune parameters such as the fish interferon system. in this study we evaluate the effect of two vegetable oils (linseed and soybean) on gilthead sea bream mx expression and other innate immune parameters. experimental diets were f ... | 2008 | 18158252 |
| susceptibility of photobacterium damselae subsp. piscicida strains isolated from senegalese sole, solea senegalensis kaup, and gilthead seabream, sparus aurata l., to several antibacterial agents. | 2008 | 18086037 | |
| high density and food deprivation affect arginine vasotocin, isotocin and melatonin in gilthead sea bream (sparus auratus). | arginine vasotocin (avt) and isotocin (it) levels in plasma and pituitary, and melatonin (mel) levels in plasma were determined in gilthead sea bream (sparus auratus) subjected to two different types of stress: i) high density (hd) and ii) food deprivation (nf: non-fed). fishes were randomly assigned to one of 4 treatments that lasted for 14 days: 1) fed fish under normal low density (nd, 4 kg m(-3)); 2) non-fed (nf) fish under nd; 3) fed fish under high density (hd, 70 kg m(-3)); and 4) non-fed ... | 2008 | 18054261 |
| t cell receptor beta chain from sea bream (sparus aurata): molecular cloning, expression and modelling of the complexes with mhc class i. | the t cell receptor is a fundamental mediator of the adaptive immune responses, since tr alphabeta on t cells recognize foreign structures (peptides derived from processed antigens) bound to the major histocompatibility complex (mhc) on apc cells. in the present study, we report the cloning of six trb chains cdna sequences from gilthead sea bream (sparus aurata), a fish of high economical impact in south mediterranean aquaculture. the v-beta domains have the canonical features of known teleost a ... | 2008 | 18035419 |
| hepatic glucokinase and glucose-6-phosphatase responses to dietary glucose and starch in gilthead sea bream (sparus aurata) juveniles reared at two temperatures. | the effects of carbohydrate sources/complexity and rearing temperature on hepatic glucokinase (gk) and glucose-6-phosphatase (g6pase) activities and gene expression were studied in gilthead sea bream juveniles. two isonitrogenous (50% crude protein) and isolipidic (19% crude lipids) diets were formulated to contain 20% waxy maize starch or 20% glucose. triplicate groups of fish (63.5 g initial body weight) were fed each diet to near satiation during four weeks at 18 degrees c or 25 degrees c. gr ... | 2008 | 18023221 |
| persistence of duplicated pac1 receptors in the teleost, sparus auratus. | : duplicated genes are common in vertebrate genomes. their persistence is assumed to be either a consequence of gain of novel function (neofunctionalisation) or partitioning of the function of the ancestral molecule (sub-functionalisation). surprisingly few studies have evaluated the extent of such modifications despite the numerous duplicated receptor and ligand genes identified in vertebrate genomes to date. in order to study the importance of function in the maintenance of duplicated genes, s ... | 2007 | 17997850 |
| classification of gilthead sea bream (sparus aurata) from 1h nmr lipid profiling combined with principal component and linear discriminant analysis. | the combination of (1)h nmr fingerprinting of lipids from gilthead sea bream (sparus aurata) with nonsupervised and supervised multivariate analysis was applied to differentiate wild and farmed fish and to classify farmed specimen according to their areas of production belonging to the mediterranean basin. principal component analysis (pca) applied on processed (1)h nmr profiles made a clear distinction between wild and farmed samples. linear discriminant analysis (lda) allowed classification of ... | 2007 | 17970589 |
| phenotypic, serological and genetic characterization of pseudomonas anguilliseptica strains isolated from cod, gadus morhua l., in northern europe. | the biochemical, serological and genetic characteristics of six strains of pseudomonas anguilliseptica isolated from cod, gadus morhua, in scotland were compared to well characterized isolates of this same bacterial species but of different origin. biochemical and physiological analyses showed that this group of isolates was highly homogeneous, their characteristics matching previous descriptions of the pathogen. similar results were obtained for the six cod isolates in the serological assays, a ... | 2007 | 17958609 |
| biomagnification of organochlorine pollutants in farmed and wild gilthead sea bream (sparus aurata) and stable isotope characterization of the trophic chains. | organochlorine pollutants (pesticides and polychlorinated biphenyls) were analysed in farmed and wild gilthead sea bream (sparus aurata) tissues (white muscle and liver) from the western mediterranean (spain) and in their diets. determination was carried out by gas chromatography coupled to tandem mass spectrometry after clean up of the fatty extracts by normal phase hplc, with detection limits around 0.1 ng/g. carbon and nitrogen stable isotope ratios were also determined in the samples. organo ... | 2008 | 17945331 |
| mitigation of lethal effects of karlodinium veneficum and k. armiger on sparus aurata: changes in haematocrit and plasma osmolality. | between january and april 2000, several experiments were performed during a karlodinium spp. proliferation in alfacs bay (ebro delta, nw mediterranean) to determine the effects of these dinoflagellates on sea bream sparus aurata cultivated in the area. moribund fish showed an increase in plasma osmolality together with a decrease in the haematocrit percentage compared to control fish. the efficacy of copper sulphate, hydrogen peroxide, potassium permanganate and formalin against karlodinium spp. ... | 2007 | 17933397 |
| natural occurrence of metallothioneinlike proteins in liver tissues of four fish species from the northeast mediterranean sea. | fish with different ecological needs were captured from the northeast mediterranean sea to determine metallothioneinlike proteins in their livers. heat-treated liver samples were run on a column packed with sephadex g-75 (sigma, germany) for subsequent determination of metals, sulfhydryl, and absorbance at 254 and 280 nm. liver cytosols of sparus auratus and mullus barbatus had three heat-stable protein peaks, whereas mugil cephalus and atherina hepsetus had two. the third protein peak, containi ... | 2007 | 17910363 |
| maternal transfer of organochlorine compounds to oocytes in wild and farmed gilthead sea bream (sparus aurata). | to investigate the transfer of lipophilic organochlorine compounds to the gonads in gilthead sea bream females, liver and oocytes from wild and farmed females, just before spawning, were analysed by gas chromatography coupled to tandem mass spectrometry after clean-up of the fatty extracts by normal phase hplc. this analytical methodology allowed us to achieve limits of detection of around 0.1 ng/g. data obtained show that the chemicals studied are transferred from the liver to oocytes in ratios ... | 2008 | 17720218 |
| diversity of shewanella population in fish sparus aurata harvested in the aegean sea. | to study the diversity of shewanella population in sparus aurata fish harvested in the aegean sea, as well as to elucidate the influence of fish storage conditions on the selection in shewanella strains. | 2007 | 17714405 |
| characterization of the de novo biosynthetic enzyme of platelet activating factor, ddt-insensitive cholinephosphotransferase, of human mesangial cells. | platelet activating factor (paf), a potent inflammatory mediator, is implicated in several proinflammatory/inflammatory diseases such as glomerulonephritis, glomerulosclerosis, atherosclerosis, cancer, allergy, and diabetes. paf can be produced by several renal cells under appropriate stimuli and it is thought to be implicated in renal diseases. the aim of this study is the characterization of dtt-insensitive cholinephosphotransferase (paf-cpt) of human mesangial cell (hmc), the main regulatory ... | 2007 | 17710109 |
| role of sp1 and srebp-1a in the insulin-mediated regulation of glucokinase transcription in the liver of gilthead sea bream (sparus aurata). | insulin induction of glucokinase (gck) transcription in the liver is essential for maintaining glucose homeostasis. to study the molecular mechanism underlying the regulation of hepatic gck expression in the carnivorous fish gilthead sea bream (sparus aurata), we analysed the role of sterol regulatory element binding protein-1a (srebp-1a) and specificity protein (sp) 1 in insulin-dependent gck transcription. transient transfection experiments performed in hepg2 cells and electrophoretic mobility ... | 2008 | 17686483 |
| simultaneous determination of selected veterinary antibiotics in gilthead seabream (sparus aurata) by liquid chromatography-mass spectrometry. | a method was optimised and validated for simultaneous monitoring of several drugs of different classes of antibiotics such as quinolones (oxilinic acid and flumequine), tetracyclines (oxytetracycline), sulfonamides (sulfadiazine) and trimethoprim in fish muscle and skin. the method is based on solid-liquid extraction without further sample clean up followed by liquid chromatography-mass spectrometry (lc-ms) determination with electrospray ion source (esi) in positive mode. the limits of quantifi ... | 2007 | 17644050 |
| co-expression of igfs and gh receptors (ghrs) in gilthead sea bream (sparus aurata l.): sequence analysis of the ghr-flanking region. | the tissue-specific expression of igfs and gh receptors (ghrs) was analyzed in gilthead sea bream (sparus aurata l.) as an attempt to understand the functional partitioning of duplicated ghrs on the regulation offish growth by season and aging. gene transcripts were measured in liver, muscle, and adipose tissue by means of quantitative real-time pcr assays. in juvenile fish, concurrent increases in circulating levels of gh and igf-i and hepatic mrna levels of igf-i and ghr-i were evidenced with ... | 2007 | 17641285 |
| effect of the pineal hormone melatonin on teleost fish phagocyte innate immune responses after in vitro treatment. | although neuroendocrine-immune system interaction has been shown in teleost fish, no study has evaluated the role of melatonin (mel) on fish immune response even considering that it is affected by the photoperiod. gilthead seabream (sparus aurata l.) and sea bass (dicentrarchus labrax l.) head-kidney leucocytes were incubated with mel (0-control-, 20 pm-400 microm) and leucocyte viability and main innate cellular immune parameters were evaluated. overall, seabream and sea bass head-kidney leucoc ... | 2007 | 17620289 |
| modelling metallothionein induction in the liver of sparus aurata exposed to metal-contaminated sediments. | metallothionein (mt) in the liver of gilthead seabreams (sparus aurata l., 1758) exposed to sado estuary (portugal) sediments was quantified to assess the mt induction potential as a biomarker of sediment-based contamination by copper (cu), cadmium (cd), lead (pb) and arsenic (as). sediments were collected from two control sites and four sites with different levels of contamination. sediment cu, cd, pb, as, total organic matter (tom) and fine fraction (ff) levels were determined. generalized lin ... | 2008 | 17617458 |
| molecular and functional characterization of gilthead seabream sparus aurata caspase-1: the first identification of an inflammatory caspase in fish. | caspases are a family of cysteine proteases that fulfil critical roles in mammalian apoptosis and in the proteolytic activation of cytokines. in humans, the caspase family includes 13 members whose functions seem to correlate with their phylogenetic relationship. they are classified into two main groups, the cell death (apoptotic) and the inflammatory caspases. caspase-1 is the best characterized inflammatory caspase and is responsible for the processing of interleukin-1beta (il-1beta), il-18 an ... | 2008 | 17610954 |
| insulin regulation of lipoprotein lipase (lpl) activity and expression in gilthead sea bream (sparus aurata). | lipoprotein lipase (lpl) is a key enzyme in lipoprotein metabolism by virtue of its capacity to hydrolyze triglycerides circulating in the form of lipoprotein particles. here we analyzed the fasting effects of lpl in gilthead sea bream (sparus aurata) and also present the first study in fish of the role of insulin as a potential modulator of both lpl activity and expression. fasting for 2 weeks provoked a clear decrease in adipose tissue lpl activity, concomitant with lower levels of plasma insu ... | 2007 | 17600746 |
| modelling the effect of high pressure on the inactivation kinetics of a pressure-resistant strain of pediococcus damnosus in phosphate buffer and gilt-head seabream (sparus aurata). | the aim of this research was to: (i) determine the inactivation pattern of a pressure-resistant strain of pediococcus damnosus by high hydrostatic pressure in phosphate buffer (ph 6.7) and gilt-head seabream using the linear, biphasic and weibull models; and (ii) validate the applicability of the weibull model to predict survival curves at other experimental pressure levels. | 2007 | 17578414 |
| nodavirus increases the expression of mx and inflammatory cytokines in fish brain. | nodavirus has become a serious pathogen for a wide range of cultured marine fish species. in the present work, the expression of genes related to immune and inflammatory responses of sea bream (sparus aurata l.), considered as non susceptible species, was studied both in vitro and in vivo. no replication of the virus was observed in head kidney macrophages and blood leukocytes. moreover, the enhancement of expression of several immune genes (tumor necrosis factor alpha (tnfalpha), interleukin-1- ... | 2008 | 17543386 |
| changes in cathepsin gene expression and relative enzymatic activity during gilthead sea bream oogenesis. | the aim of this study was to provide evidence on the modulation of lysosomal enzymes in terms of both gene expression and enzymatic activity during follicle maturation. for this purpose three lysosomal enzymes, cathepsins b, d, and l, were studied in relation to yolk formation and degradation, during the main phases of ovarian follicle growth in the pelagophil species, the sea bream sparus aurata. specific attention was focused on the gene expression quantification method, on the assay of enzyma ... | 2008 | 17538957 |
| a pth/pthrp receptor antagonist blocks the hypercalcemic response to estradiol-17beta. | estradiol (e(2)) increases circulating calcium and phosphate levels in fish, thus acting as a hypercalcemic and hyperphosphatemic factor during periods of high calcium requirements, such as during vitellogenesis. since parathyroid hormone (pth)-related protein (pthrp) has been shown to be calciotropic in fish, we hypothesized that the two hormones could be mediating the same process. sea bream (sparus auratus) juveniles receiving a single intraperitoneal injection of piscine pthrp(1-34) showed a ... | 2007 | 17537843 |
| parathyroid hormone-related protein and calcium regulation in vitamin d-deficient sea bream (sparus auratus). | gilthead sea bream (sparus auratus l.) were fed a vitamin d-deficient diet for 22 weeks. growth rate, whole body mineral pools and calcium balance were determined. plasma parathyroid hormone-related protein (pthrp) and calcitriol levels were assessed. expression of mrna for pthrp and pth1r was quantified in gills and hypophysis. fish on vitamin d-deficient diet (d- fish) showed reduced growth and lower calcium turnover (calcium influx, efflux and accumulation rates decreased) and unaltered plasm ... | 2007 | 17535884 |
| stable isotope determination in wild and farmed gilthead sea bream (sparus aurata) tissues from the western mediterranean. | stable isotopes of carbon and nitrogen (delta(13)c and delta(15)n) have been determined in wild and farmed gilthead sea bream (sparus aurata) samples of white and red muscle, liver, gills and gonads. first, delta(13)c and delta(15)n values were determined in samples with and without lipid removal to check the possible effect of lipid content on the stable isotope values of the different tissues studied. differences were found for delta(13)c in all tissues studied apart from white muscle of wild ... | 2007 | 17532023 |
| survival of vibrio alginolyticus in seawater and retention of virulence of its starved cells. | the aim of this study was to evaluate the survival responses of four strains of vibrio alginolyticus in seawater under starvation conditions. we used microcosms containing sterilised seawater and incubated at ambient temperature (22-25 degrees c). v. alginolyticus maintained its culturability for at least nine months. long-term-starved cells showed an absence or a decrease in their enzymatic activities. resuscitation assays of viable but non-culturable (vbnc) cells were conducted and the recover ... | 2007 | 17524473 |
| conjugated linoleic acid affects lipid composition, metabolism, and gene expression in gilthead sea bream (sparus aurata l). | to maximize growth, farmed fish are fed high-fat diets, which can lead to high tissue lipid concentrations that have an impact on quality. the intake of conjugated linoleic acid (cla) reduces body fat in mammals and this study was undertaken to determine the effects of dietary cla on growth, composition, and postprandial metabolic variables in sea bream. fish were fed 3 diets containing 48 g/100 g protein and 24 g/100 g fat, including fish oil supplemented with 0 (control), 2, or 4% cla for 12 w ... | 2007 | 17513392 |
| four parasitic crustacean species from marine fishes of turkey. | the aim of this work was to present a preliminary knowledge of the parasitic copepods of marine fish of turkey. in this study, four parasitic crustaceans were reported from five different fish species found in turkish seas: lepeophtheirus europaensis (zeddam, berrebi, renaud, raibaut & gabrion, 1988) was found on the gills of the european flounder, platichtys flesus (linnaeus, 1758 (pleuronectidae); nerocila bivittata (risso, 1816) on caudal peduncles of east atlantic peacock wrasse, symphodus t ... | 2007 | 17471420 |
| molecular characterization of a cdna from the gilthead sea bream (sparus aurata) encoding a fish prion protein. | we have identified and characterized a cdna from the brain tissue of the highly commercial marine fish species, the gilthead sea bream (sparus aurata), which encodes a 496 amino acid residue protein sharing the organizational and structural features of the mammalian prion proteins. tissue mrna expression analyses revealed the presence of this transcript in various tissues of the gilthead sea bream including the brain, the spleen, and the heart. sequence comparison and phylogenetic analysis showe ... | 2007 | 17468028 |
| authenticating production origin of gilthead sea bream (sparus aurata) by chemical and isotopic fingerprinting. | recent eu legislation (ec/2065/2001) requires that fish products, of wild and farmed origin, must provide consumer information that describes geographical origin and production method. the aim of the present study was to establish methods that could reliably differentiate between wild and farmed european gilthead sea bream (sparus aurata). the methods that were chosen were based on chemical and stable isotopic analysis of the readily accessible lipid fraction. this study examined fatty acid prof ... | 2007 | 17464521 |
| sp1 and sp3 regulate glucokinase gene transcription in the liver of gilthead sea bream (sparus aurata). | to better understand the transcriptional machinery that governs glucokinase (gck) expression, we have cloned and characterized the proximal promoter region of gck from gilthead sea bream (sparus aurata). the 5'-flanking region of gck was isolated by chromosome walking. smart race-pcr allowed us to locate the transcription start site 98 bp (bp) upstream from the translational start. transfection analysis in hepg2 cells revealed the presence of a functional promoter in the 1397 bp 5'-flanking isol ... | 2007 | 17446237 |
| melatonin synthesis under calcium constraint in gilthead sea bream (sparus auratus l.). | brain or blood plasma melatonin was analysed as a measure for pineal melatonin production in sea bream. access to calcium was limited by diluting the seawater to 2.5 per thousand and removing calcium from the diet or by prolonged feeding of vitamin d-deficient diet. interactions/relations between melatonin and calcium balance and the hypercalcemic endocrines pthrp and calcitriol were assessed. restricting calcium availability in both water and diet had no effect on plasma melatonin, but when cal ... | 2008 | 17434515 |
| the response of digestive proteases to abrupt salinity decrease in the euryhaline sparid sparus aurata l. | the response of the digestive proteases to abrupt salinity change was studied in juvenile gilthead sea bream (sparus aurata) for 15 days after transfer from 33 per thousand to 21 per thousand. salinity decrease affected significantly neither the activity of total acid proteases in stomach, nor the activities of total alkaline proteases and major serine proteases--trypsin and chymotrypsin--in the alkaline part of the intestine. the activity of the major proteases was significantly different betwe ... | 2007 | 17407829 |
| effect of host factors and experimental conditions on the horizontal transmission of enteromyxum leei (myxozoa) to gilthead sea bream, sparus aurata l., and european sea bass, dicentrarchus labrax (l.). | 2007 | 17394527 | |
| emergence of pathogenic betanodaviruses belonging to the sjnnv genogroup in farmed fish species from the iberian peninsula. | betanodaviruses are the causative agents of viral nervous necrosis (vnn) or viral encephalopathy and retinopathy (ver) in cultured marine fish. based on the rna2 gene fish nodaviruses have been traditionally classified into four different genotypes and recently a fifth genotype has been proposed. this study presents sequencing data of 24 new nodaviruses obtained from three different fish species: sea bass, dicentrarchux labrax (l.), sea bream, sparus aurata l., and senegalese sole, solea senegal ... | 2007 | 17394524 |
| sequence analysis and tissue expression pattern of sparus aurata chymotrypsinogens and trypsinogen. | two apparently full-length cdna clones encoding chymotrypsinogens i and ii (chtri, 1022 bp; chtrii, 909 bp) and one cdna clone encoding trypsinogen ii (trpii, 848 bp) were isolated from a cdna library prepared from gilthead sea bream (sparus aurata) liver. the deduced amino acid sequences of the isolated cdnas contain highly conserved residues essential for serine protease catalytic activity and conformational maintenance. the deduced amino acid sequences of chtri and chtrii are 261 aa and 277 a ... | 2007 | 17341447 |
| an unidentified epi-epithelial myxosporean in the intestine of gilthead sea bream sparus aurata l. | in the course of experimental infections of gilthead sea bream sparus aurata with the myxozoan enteromyxum leei, stages of an unidentified myxozoan were observed attached to the intestinal brush border of some fish. infection levels of the parasite, which was named "epi-epithelial myxosporean" (eem) were recorded, and its structure was studied by light microscopy (lm) and electron microscopy (em). in situ hybridisation (ish) probes specific for e. leei were developed and used to differentiate be ... | 2007 | 17333277 |
| a gene-based radiation hybrid map of the gilthead sea bream sparus aurata refines and exploits conserved synteny with tetraodon nigroviridis. | comparative teleost studies are of great interest since they are important in aquaculture and in evolutionary issues. comparing genomes of fully sequenced model fish species with those of farmed fish species through comparative mapping offers shortcuts for quantitative trait loci (qtl) detections and for studying genome evolution through the identification of regions of conserved synteny in teleosts. here a comparative mapping study is presented by radiation hybrid (rh) mapping genes of the gilt ... | 2007 | 17286862 |
| effects of 17beta-estradiol and 4-nonylphenol on osmoregulation and hepatic enzymes in gilthead sea bream (sparus auratus). | sexually immature sparus auratus were injected intraperitoneally with coconut oil either alone (control) or containing 17beta-estradiol (e2, 10 microg/g body mass) or 4-nonyphenol (4-np, 100 and 200 microg/g body mass) and sampled 10 days later. gill and kidney na(+),k(+)-atpase activities, plasma levels of e2 and cortisol, plasma osmolites (osmolality, sodium and chloride) and metabolites (glucose, lactate, proteins and triglycerides) were examined. livers were used for measuring hepatosomatic ... | 2007 | 17251064 |
| the type ii interleukin-1 receptor (il-1rii) of the bony fish gilthead seabream sparus aurata is strongly induced after infection and tightly regulated at transcriptional and post-transcriptional levels. | interleukin-1beta (il-1beta) is the prototypic pro-inflammatory cytokine. all the biological effects of il-1beta are mediated through interaction with type 1 il-1 receptor (il-1ri), whereas another receptor, called type 2 il-1r (il-1rii), lacks an intracellular signalling domain and acts as a decoy receptor that down-regulates responses to il-1beta. although both receptors are present in bony fish, their expression and biological role in the regulation of il-1beta activity in non-mammalian verte ... | 2007 | 17234266 |
| purification and kinetic properties of 6-phosphofructo-1-kinase from gilthead sea bream muscle. | the kinetic properties of 6-phosphofructo-1-kinase (pfk) from skeletal muscle (pfkm) of gilthead sea bream (sparus aurata) were studied, after 10,900-fold purification to homogeneity. the native enzyme had an apparent molecular mass of 662 kda and is composed of 81 kda subunits, suggesting a homooctameric structure. at physiological ph, s. aurata pfkm exhibited sigmoidal kinetics for the substrates, fructose-6-phosphate (fru-6-p) and atp. fructose-2,6-bisphosphate (fru-2,6-p(2)) converted the sa ... | 2007 | 17229526 |
| ici 182,780 has agonistic effects and synergizes with estradiol-17 beta in fish liver, but not in testis. | ici 182,780 (ici) belongs to a new class of antiestrogens developed to be pure estrogen antagonists and, in addition to its therapeutic use, it has been used to knock-out estrogen and estrogen receptor (er) actions in several mammalian species. in the present study, the effects and mechanism of action of ici were investigated in the teleost fish, sea bream (sparus auratus). | 2006 | 17192186 |
| tumour necrosis factor (tnf)alpha as a regulator of fat tissue mass in the mediterranean gilthead sea bream (sparus aurata l.). | the study was undertaken to analyze the lipolytic effect and transcriptional regulation of tumour necrosis factor (tnf)alpha in gilthead sea bream (sparus aurata l.). the study was also focused on the transcriptional regulation and analysis of the 5-flanking region of lipoprotein lipase (lpl) in an attempt to identify cis-regulatory elements that would support the tnfalpha-mediated effects. the lipolytic effect of tnfalpha was evidenced by the increased release of glycerol in the culture medium ... | 2007 | 17189708 |
| suppression subtraction hybridization (ssh) and macroarray techniques reveal differential gene expression profiles in brain of sea bream infected with nodavirus. | despite of the impact that viruses have on aquatic organisms, relatively little is known on how fish fight against these infections. in this work, the brain gene expression pattern of sea bream (sparus aurata) in response to nodavirus infection was investigated. we used the suppression subtractive hybridization (ssh) method to generate a subtracted cdna library enriched with gene transcripts differentially expressed after 1 day post-infection. some of the ests from the infected tissues fell in g ... | 2007 | 17188359 |
| host switch of lamellodiscus elegans (monogenea: monopisthocotylea) and sparicotyle chrysophrii (monogenea: polyopisthocotylea) between cage-reared sparids. | sharpsnout bream (diplodus puntazzo) has been used in adriatic aquaculture for less than a decade, but the decreasing trend of rearing this species will probably result in its complete substitution by more exploited sea bream (sparus aurata). only two facilities still rear both fish species in neighbouring cages in monoculture. a switch of parasites was observed between sparids during monitoring of the gill monogeneans of farmed fish. in wild fish of the adriatic sea, lamellodiscus elegans (mono ... | 2007 | 17187241 |
| transcriptional regulation of arylalkylamine-n-acetyltransferase-2 gene in the pineal gland of the gilthead seabream. | pineal serotonin-n-acetyltransferase (arylalkylamine-n-acetyltransferase; aanat) is considered the key enzyme in the generation of circulating melatonin rhythms; the rate of melatonin production is determined by aanat activity. in all the examined species, aanat activity is regulated at the post-translational level and, to a variable degree, also at the transcriptional level. here, the transcriptional regulation of pineal aanat (aanat2) of the gilthead seabream (sparus aurata) was investigated. ... | 2007 | 17184485 |
| the use of stable isotope ratio analyses to discriminate wild and farmed gilthead sea bream (sparus aurata). | continuous flow isotope ratio mass spectrometry (cf-irms) has been used to analyze samples of gilthead sea bream (g.s.b.) (sparus aurata) of known geographical origin (four countries on the mediterranean sea) from wild and farmed sources (40 farmed and 10 wild). delta(13)c and delta(15)n values have been measured on muscle samples as these are the most informative parameters of the diet of the animals. both stable isotopes are indicators of the origin of the fish: delta(13)c giving a tool to dis ... | 2007 | 17171779 |
| kinetic of biomarker responses in juveniles of the fish sparus aurata exposed to contaminated sediments. | sediments in the national park of the atlantic islands (galicia, spain) were affected by the spill of the tanker prestige (november, 2002) and still present high levels of polycyclic aromatic hydrocarbons. the adverse effects associated with the contaminants in sediments were tested using a chronic bioassay, exposing juveniles of the fish sparus aurata (seabream). a toxicokinetic approach is proposed to evaluate sediment quality by linking chemical and ecotoxicological data along the time. sedim ... | 2007 | 17171271 |
| evaluation of immunological parameters in farmed gilthead sea bream, sparus aurata l., before and during outbreaks of 'winter syndrome'. | in recent years, 'winter syndrome' has caused severe economic losses in many marine farms in southern europe. this study compared the activity of the immune system in healthy, asymptomatic and diseased gilthead sea bream. serum protein and immunoglobulin content were evaluated as well as serum complement activity from these groups of fish. differential leucocyte counts were also determined. immunological assays were performed to determine intra- and extracellular 'respiratory burst' activity as ... | 2006 | 17169115 |
| experimental transmission of enteromyxum leei to freshwater fish. | the myxosporean enteromyxum leei is known to infect a wide range of marine fish hosts. the objective of the present study was to determine whether freshwater fish species are also receptive hosts to this parasite. seventeen species of freshwater fish were experimentally fed e. leei-infected gut tissue from donor gilthead sea bream sparus aurata obtained from a commercial sea bream cage farm. four of the tested species, tiger barb puntius tetrazona, zebra danio danio rerio, oscar astronotus ocell ... | 2006 | 17140140 |
| fine structure of spermatozoa in the gilthead sea bream (sparus aurata linnaeus, 1758) (perciformes, sparidae). | scanning and transmission electron microscopy were used to investigate the fine structure of the sperm of the sparid fish sparus aurata l. the mature spermatozoon of gilthead sea bream belongs, like that of the other sparid fish, to a "type i" as defined by mattei (1970). it has a spherical head which lacks an acrosome, a short, irregularly-shaped midpiece and a long cylindrical tail. the nucleus reveals a deep invagination (nuclear fossa) in which the centriolar complex is located. the two cent ... | 2007 | 17128413 |
| regulation of troponin t expression during muscle development in sea bream sparus auratus linnaeus: the potential role of thyroid hormones. | in the sea bream sparus auratus three stage-specific fast troponin t (ftnt) isoforms have been cloned and correspond to embryonic-, larval- and adult-specific isoforms. characterisation, using database searches, of the putative genomic organisation of fugu rubripes and tetraodon nigroviridis ftnt indicates that alternative exon splicing in the 5 region of the gene generates the different isoforms. moreover, comparison of teleost ftnts suggests that alternative splicing of ftnt appears to be comm ... | 2006 | 17114408 |
| cell-mediated cytotoxicity is the main innate immune mechanism involved in the cellular defence of gilthead seabream (teleostei: sparidae) against enteromyxum leei (myxozoa). | the cellular innate immune response of gilthead seabream (sparus aurata l.) against the myxozoan enteromyxum leei was studied. enteromyxosis was transmitted by maintaining uninfected fish (recipients) together with infected animals. a group of fish not exposed to the infection served as controls. after 10, 22, 38, 52 and 108 days, control and recipient fish were sampled and leucocyte subpopulations and cellular immune responses (leucocyte peroxidases, phagocytosis, respiratory burst and cytotoxi ... | 2006 | 17096645 |
| isolation and characterization of a fish f-type lectin from gilt head bream (sparus aurata) serum. | a novel fucose-binding lectin, designated saufbp32, was purified by affinity chromatography on fucose-agarose, from the serum of the gilt head bream sparus aurata. electrophoretic mobility of the subunit revealed apparent molecular weights of 35 and 30 kda under reducing and non-reducing conditions, respectively. size exclusion analysis suggests that the native lectin is a monomer under the selected experimental conditions. agglutinating activity towards rabbit erythrocytes was not significantly ... | 2007 | 17095163 |
| vegetable oils affect the composition of lipoproteins in sea bream (sparus aurata). | the aim of the present study was to determine the influence of the dietary fatty acid profile on the lipoprotein composition in sea bream fed different vegetable oils. six experimental diets were formulated combining fish oil with three vegetable oils (soybean, rapeseed, linseed) in order to obtain 60-80 % (w/w) fish-oil replacement. vldl, ldl and hdl in plasma samples were obtained by sequential centrifugal flotation. the lipid class, protein content and fatty acid composition of each lipoprote ... | 2006 | 17092370 |
| vanadium distribution, lipid peroxidation and oxidative stress markers upon decavanadate in vivo administration. | the contribution of decameric vanadate species to vanadate toxic effects in cardiac muscle was studied following an intravenous administration of a decavanadate solution (1mm total vanadium) in sparus aurata. although decameric vanadate is unstable in the assay medium, it decomposes with a half-life time of 16 allowing studying its effects not only in vitro but also in vivo. after 1, 6 and 12h upon decavanadate administration the increase of vanadium in blood plasma, red blood cells and in cardi ... | 2007 | 17030392 |
| effects of gutting and ungutting on microbiological, chemical, and sensory properties of aquacultured sea bream (sparus aurata) and sea bass (dicentrarchus labrax) stored in ice. | the effect of gutting and ungutting on microbiological, chemical, and sensory properties of aqua-cultured sea bream (sparus aurata) and sea bass (dicentrarchus labrax) stored in ice were studied. the total viable mesophilic and psychrophilic bacterial counts increased throughout the storage period of gutted and ungutted sea bream and sea bass. the mesophilic counts reached 8.19 log cfu/g for ungutted sea bream and 7.93 log cfu/g for ungutted sea bass after 14 days of storage. the mesophilic coun ... | 2006 | 16954060 |
| a genetic linkage map of the hermaphrodite teleost fish sparus aurata l. | the gilthead sea bream (sparus aurata l.) is a marine fish of great importance for fisheries and aquaculture. it has also a peculiar sex-determination system, being a protandrous hermaphrodite. here we report the construction of a first-generation genetic linkage map for s. aurata, based on 204 microsatellite markers. twenty-six linkage groups (lg) were found. the total map length was 1241.9 cm. the ratio between sex-specific map lengths was 1:1.2 (male:female). comparison with a preliminary rad ... | 2006 | 16951080 |
| arginine vasotocin, isotocin and melatonin responses following acclimation of gilthead sea bream (sparus aurata) to different environmental salinities. | gilthead sea bream (sparus aurata) is a euryhaline species with a capacity to cope with demands in a wide range of salinities and thus is a perfect model-fish to study osmoregulatory responses to salinity-adaptive processes and their hormonal control. immature sea bream acclimated to different salinities, i.e. sw (38 per thousand), lsw (5 per thousand) and hsw (55 per thousand), were kept at 18 degrees c under natural photoperiod. arginine vasotocin (avt) and isotocin (it) in plasma and pituitar ... | 2006 | 16949846 |
| effect of photoperiod on the fish innate immune system: a link between fish pineal gland and the immune system. | the pineal gland via its secretory product, melatonin, influences the light-dark rhythm in most vertebrates including fish. apart from the information concerning this circadian rhythm, the interrelation of the melatonin with other physiological processes has not been considered in fish. thus, we evaluated the changes in the humoral innate immune system of seabream (sparus aurata l.) and sea bass (dicentrarchus labrax l.) specimens exposed to a constant light-dark photoperiod (12 hr l:12 hr d). s ... | 2006 | 16948787 |
| pthrp regulation and calcium balance in sea bream (sparus auratus l.) under calcium constraint. | juvenile gilthead sea bream were exposed to diluted seawater (2.5 per thousand salinity; dsw) for 3 h or, in a second experiment, acclimated to dsw and fed a control or calcium-deficient diet for 30 days. branchial ca2+ influx, drinking rate and plasma calcium levels were assessed. sea bream plasma parathyroid hormone related protein (spthrp) was measured, and mrnas of pthrp, its main receptor, pth1r, and the calcium-sensing receptor (casr) were quantified in osmoregulatory tissues and the pitui ... | 2006 | 16943495 |
| interactions between bacteria and cryptosporidium molnari in gilthead sea bream (sparus aurata) under farm and laboratory conditions. | the possible interaction of cryptosporidium molnari and bacteria in gilthead sea bream (sparus aurata) was studied. epidemiological data from a pathological survey under farm conditions were analyzed. in addition, parasite and bacteria burdens were studied in experimental models in which naturally and experimentally parasitized fish were challenged with a particular strain of vibrio harveyi (h57). all the bacteria species present were studied. under farm conditions, the parasite was more prevale ... | 2006 | 16934406 |
| bioaccumulation of some heavy metals in different tissues of dicentrarchus labrax l, 1758, sparus aurata l, 1758 and mugil cephalus l, 1758 from the camlik lagoon of the eastern coast of mediterranean (turkey). | in this study, bioaccumulation of the heavy metals (fe, zn, cd) in the liver, gill, gonad and muscle tissues of dicentrarchus labrax, mugil cephalus and sparus aurata were investigated seasonally. camlik is the important fisheries lagoon located in the iskenderun bay, mediterranean coast of turkey. due to heavy industrial and agricultural activities in the region, the bay has the polluted coastal waters of turkey. therefore, the levels of cadmium, zinc, iron were determined in the muscle in each ... | 2006 | 16897534 |
| parasitic diseases of marine fish: epidemiological and sanitary considerations. | over recent decades, parasitic diseases have been increasingly considered a sanitary and economic threat to mediterranean aquaculture. in order to monitor the distribution of parasites in cultured marine fish from italy and study their pathogenic effects on the host, a three-year survey based on parasitological and histopathological exams was carried out on 2141 subjects from eleven fish species and coming from different farming systems (extensive, intensive inland farms, inshore floating cages, ... | 2006 | 16881387 |
| virulence and enterobacterial repetitive intergenic consensus pcr of vibrio alginolyticus strains isolated from tunisian cultured gilthead sea bream and sea bass outbreaks. | vibrio alginolyticus was isolated from the internal organs of diseased gilthead sea bream (sparus aurata) and sea bass (dicentrarchus labrax) cultured in two fish farms located on the tunisian mediterranean coast, from 2003 to 2005. after phenotypic characterisation, a selection of 34 isolates from gilthead sea bream and sea bass were molecularly typed by repetitive intergenic consensus pcr (eric-pcr) showing a high polymorphism among the isolated strains (19 genotypes). most of the isolates wer ... | 2006 | 16870360 |
| early local and systemic innate immune responses in the teleost gilthead seabream after intraperitoneal injection of whole yeast cells. | the early cellular innate immune responses of the teleost gilthead seabream (sparus aurata l.) against whole yeast cells were studied. fish received a single intraperitoneal (i.p.) injection of saccharomyces cerevisiae and leukocyte mobilization, degranulation, peroxidase content, respiratory burst, phagocytic and cytotoxic activities were assayed in both head-kidney leukocytes (hkls) and peritoneal exudate leukocytes (pels). the total number of pels significantly increased from 4 h post-injecti ... | 2007 | 16843011 |
| pthrp potentiating estradiol-induced vitellogenesis in sea bream (sparus auratus, l.). | in fish, vitellogenin is an important nutritional precursor protein produced solely in the liver and released into the blood where it binds calcium. in the gilthead sea bream (sparus auratus) 17beta-estradiol (e2) plays an important role in the synthesis of vitellogenin, but also the pituitary hormones prolactin (prl) and growth hormone (gh) can stimulate vitellogenin induction in fish. considering the emerging involvement of pthrp in fish calcium metabolism and the importance of calcium regulat ... | 2006 | 16839552 |
| modulation of acth-induced cortisol release by polyunsaturated fatty acids in interrenal cells from gilthead seabream, sparus aurata. | highly unsaturated fatty acids are essential components of cellular membranes of vertebrates and can modulate physiological processes, including membrane transport, receptor function and enzymatic activities. in gilthead sea bream, dietary deficiencies of essential fatty acids of marine fish raise the basal cortisol levels and alter the pattern of cortisol release after stress. the aim of the present study was to clarify the effect of different essential fatty acids on adrenocorticotropic hormon ... | 2006 | 16837609 |
| full-length sequence and expression analysis of toll-like receptor 9 in the gilthead seabream (sparus aurata l.). | toll-like receptors (tlrs) have recently emerged as key sensors of invading microbes, acting through recognition of pathogen-associated molecular patterns. it has been demonstrated that tlr9 is involved in the recognition of unmethylated cpg motifs in mice, humans, and pigs. we report here the full-length sequence of tlr9 cdna in the gilthead sea bream (sparus aurata l.). the predicted protein (1063 amino acids) was similar to mammalian tlr9s, showing 21 leucine-rich repeats in the extracellular ... | 2006 | 16797882 |
| influence of testosterone administration on osmoregulation and energy metabolism of gilthead sea bream sparus auratus. | the osmoregulatory and metabolic role of testosterone (t) in the euryhaline teleost sparus auratus was examined. fish were implanted with a slow-release coconut oil implant alone (control) or containing t (2 or 5microgg(-1) body weight) and sampled 1, 3, and 7 days after implantation. gill na(+),k(+)-atpase activity increased in fish treated with the lower dose of t after 7 days of treatment. kidney na(+),k(+)-atpase activity enhanced at first day post-implantation in the group treated with the ... | 2006 | 16765351 |
| parathyroid hormone-related protein regulates intestinal calcium transport in sea bream (sparus auratus). | parathyroid hormone-related protein (pthrp) is a factor associated with normal development and physiology of the nervous, cardiovascular, immune, reproductive, and musculoskeletal systems in higher vertebrates. it also stimulates whole body calcium uptake in sea bream (sparus auratus) larvae with an estimated 60% coming from intestinal uptake in seawater. the present study investigated the role of pthrp in the intestinal calcium transport in the sea bream in vitro. unidirectional mucosal-to-sero ... | 2006 | 16763076 |
| growth hormone and prolactin actions on osmoregulation and energy metabolism of gilthead sea bream (sparus auratus). | the gilthead sea bream (sparus auratus) is an euryhaline fish where prolactin (prl) and growth hormone (gh) play a role in the adaptation to different environmental salinities. to find out the role of these pituitary hormones in osmoregulation and energy metabolism, fish were implanted with slow release implants of ovine gh (ogh, 5 microg g(-1) body mass) or ovine prolactin (oprl, 5 microg g(-1) body mass), and sampled 7 days after the start of the treatment. gh increased branchial na(+),k(+)-at ... | 2006 | 16750408 |
| osmoregulatory and metabolic changes in the gilthead sea bream sparus auratus after arginine vasotocin (avt) treatment. | the influence of arginine vasotocin (avt) on osmoregulation and metabolism in gilthead sea bream sparus auratus was evaluated by two experimental approaches. in the first, seawater (sw, 36 ppt)-acclimatized fish were injected intraperitoneally with vehicle (vegetable oil) or two doses of avt (0.5 and 1 microg/g body weight). twenty-four hours later, eight fish from each group were sampled; the remaining fish were transferred to low saline water (lsw, 6 ppt, hypoosmotic test), sw (transfer contro ... | 2006 | 16737699 |
| effects of administration of probiotic strains on galt of larval gilthead seabream: immunohistochemical and ultrastructural studies. | two bacterial strains lactobacillus fructivorans (as17b), isolated from adult seabream (sparus aurata l.) gut, and lactobacillus plantarum (906), isolated from human faeces, were administered contemporaneously during seabream development using brachionus plicatilis and/or artemia salina and dry feed as vectors. experimental group a received the probiotic strains already via rotifers from day 5 post-hatch (ph), whereas treatment of group b began with artemia feeding from day 27 ph. fish were samp ... | 2007 | 16730458 |
| ontogeny of the endocrine pancreatic cells of the gilthead sea bream, sparus aurata (teleost). | the development of the gilthead sea bream, sparus aurata, endocrine pancreas was studied from hatching to 114 days, using immunocytochemical techniques. bonito insulin (ins)-, synthetic somatostatin-14 (ss-14)-, salmon somatostatin-25 (ss-25)-, mammalian somatostatin-28 (1-12) (ss-28)-, porcine glucagon (glu)-, glucagon-like peptide-1 (1-19) (glp-1)-, synthetic porcine peptide tyrosine tyrosine (pyy)-, and neuropeptide y (npy)-like immunoreactivities were demonstrated. the different types of end ... | 2006 | 16630618 |
| cortisol and parathyroid hormone-related peptide are reciprocally modulated by negative feedback. | in previous in vitro studies, we have shown that the n-terminal region of parathyroid hormone-related protein (pthrp) can stimulate cortisol production in sea bream, sparus auratus, interrenal tissue, possibly through a paracrine action. in the current study, the systemic interaction between cortisol and pthrp was studied in vivo. sustained elevated blood cortisol levels, induced either by cortisol injection or confinement stress, suppressed circulating pthrp 6 and 24-fold, respectively, by comp ... | 2006 | 16624313 |
| sterol regulatory element binding protein-1a transactivates 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene promoter. | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (pfkfb) catalyzes the synthesis and degradation of fructose-2,6-bisphosphate, a key modulator of glycolysis-gluconeogenesis. to gain insight into the molecular mechanism behind hormonal and nutritional regulation of pfkfb expression, we have cloned and characterized the proximal promoter region of the liver isoform of pfkfb (pfkfb1) from gilthead sea bream (sparus aurata). transient transfection of hepg2 cells with deleted gene promoter constr ... | 2006 | 16614080 |
| effect of heat-inactivated fish and non-fish derived probiotics on the innate immune parameters of a teleost fish (sparus aurata l.). | the in vitro effects of four heat-inactivated bacterial species on the cellular innate immune responses of the gilthead seabream (sparus aurata l.) were investigated. head-kidney leucocytes were isolated and incubated for 30 min with two bacteria isolated from seabream skin (pdp11 and 51m6; members of the vibrionaceae and in the genus shewanella) and two bacteria used as probiotics in humans and cattle (lactobacillus delbrüeckii subsp. lactis and bacillus subtilis) at 5 x 10(5), 5 x 10(6) or 5 x ... | 2006 | 16516307 |
| vegetable lipid sources affect in vitro biosynthesis of triacylglycerols and phospholipids in the intestine of sea bream (sparus aurata). | despite the good growth performance of several fish species when dietary fish oil is partly replaced by vegetable oils, recent studies have reported several types of intestinal morphological alterations in cultured fish fed high contents of vegetable lipid sources. however, the physiological process implied in these morphological changes have not been clarified yet, since alterations in the physiological mechanisms involved in the different processes of lipid absorption could be responsible for ... | 2006 | 16512929 |
| intra-adrenal interactions in fish: catecholamine stimulated cortisol release in sea bass (dicentrarchus labrax l.). | the effect of the catecholamines, adrenaline and noradrenaline, on sea bass (dicentrarchus labrax) and sea bream (sparus auratus) interrenal cortisol production was studied in vitro using a dynamic superfusion system technique. increasing concentrations of catecholamines (10(-6), 10(-8) and 10(-10) m) stimulated cortisol production in a dose-dependent manner, in sea bass only. the increase in cortisol production stimulated by adrenaline (10(-6) m) and noradrenaline (10(-6) m) was inhibited by so ... | 2006 | 16443381 |
| food deprivation alters osmoregulatory and metabolic responses to salinity acclimation in gilthead sea bream sparus auratus. | the influence of acclimation to different environmental salinities (low salinity water, lsw; seawater, sw; and hyper saline water, hsw) and feeding conditions (fed and food deprived) for 14 days was assessed on osmoregulation and energy metabolism of several tissues of gilthead sea bream sparus auratus. fish were randomly assigned to one of six treatments: fed fish in lsw, sw, and hsw, and food-deprived fish in lsw, sw, and hsw. after 14 days, plasma, liver, gills, kidney and brain were taken fo ... | 2006 | 16432730 |
| kudoa iwatai (myxosporea: multivalvulida) in wild and cultured fish in the red sea: redescription and molecular phylogeny. | gilt-head sea bream, sparus aurata l., the mediterranean's most important mariculture species, has been cultured for the last 30 yr in eilat (israeli red sea). kudoa sp. was the first myxosporean parasite reported from this species. in recent years, an increase in prevalence in both land-based and sea-cage facilities in eilat has been observed. infections with the same kudoa species appeared in cultured european sea bass dicentrarchus labrax (l.) and grey mullet mugil cephalus in the same farms, ... | 2005 | 16419765 |
| arginine vasotocin (avt) and isotocin (it) in fish brain: diurnal and seasonal variations. | an hplc assay with solid-phase extraction and fluorescence derivatization was developed for measurement of arginine vasotocin (avt) and isotocin (it) in the neural tissues of fish. the efficiency and usefulness of the method have been verified in experiments by examination of peptides concentrations in brains of three fish species. the day-night changes in neuropeptides levels have been studied in brains of adult sea bream (sparus aurata) and juvenile atlantic salmon (salmo salar). seasonal fluc ... | 2006 | 16413807 |
| toxicity and gross pathology of ivermectin bath treatment in sea bream sparus aurata, l. | the isopod parasite ceratothoa oestroides becomes hard to eradicate in intensive fish rearing systems and in addition to causing weight loss in adult fish it causes mortalities in larval and juveniles stages of sea bass and sea bream. faced with the fast emergence of this infection in adriatic finfish facilities, an effective compound had to be tested for bath treatment. ivermectin was chosen for this purpose on the basis of extensive practice in northern european countries which used ivermectin ... | 2006 | 16406587 |
| gilthead seabream ( sparus aurata l.) innate defence against the parasite enteromyxum leei (myxozoa). | the humoral innate immune response of gilthead seabream (sparus aurata l.) against the myxozoan enteromyxum leei has been studied. at 10, 22, 38, 52 and 108 days of cohabitation fish were sampled to examine gut histology and to determine serum innate immune parameters and the mrna expression of pro-inflammatory cytokines (il-1beta and tnfalpha) in head-kidney. the parasite was successfully transmitted to 45% of the recipient fish and prevalence reached a maximum (62.5%) at the last sampling time ... | 2006 | 16393358 |
| characterization of muscle-regulatory gene, myod, from flounder (paralichthys olivaceus) and analysis of its expression patterns during embryogenesis. | specification and differentiation of skeletal muscle cells are driven by the activity of genes encoding members of the myogenic regulatory factors (mrfs). in vertebrates, the mrf family includes myod, myf5, myogenin, and mrf4. the mrfs are capable of converting a variety of nonmuscle cells into myoblasts and myotubes. to better understand their roles in fish muscle development, we isolated the myod gene from flounder (paralichthys olivaceus) and analyzed its structure and patterns of expression. ... | 2006 | 16362809 |
| detection of lymphocystis disease virus (lcdv) in asymptomatic cultured gilt-head seabream (sparus aurata, l.) using an immunoblot technique. | an immunoblot technique for the detection of lymphocystis disease virus (lcdv) in naturally infected gilt-head seabream (sparus aurata, l.) has been developed. a specific antiserum against a 60 kda viral protein has been proven to be an appropriate tool for lcdv diagnosis either from inoculated cell cultures or from fish tissues using the immunoblot assay. the sensitivity of this technique varied between 10(-1) and 10(2) tcid50. lcdv has also been detected in fish tissues from both, diseased and ... | 2006 | 16298500 |
| time course of osmoregulatory and metabolic changes during osmotic acclimation in sparus auratus. | changes in different osmoregulatory and metabolic parameters over time were assessed in gills, kidney, liver and brain of gilthead sea bream sparus auratus transferred either from seawater (sw, 38 p.p.t.) to hypersaline water (hsw, 55 p.p.t.) or from sw to low salinity water (lsw, 6 p.p.t.) for 14 days. changes displayed by osmoregulatory parameters revealed two stages during hyperosmotic and hypo-osmotic acclimation: (i) an adaptive period during the first days of acclimation (1-3 days), with i ... | 2005 | 16272252 |
| identification of estrogen-responsive genes in the testis of sea bream (sparus auratus) using suppression subtractive hybridization. | there is growing evidence that estrogens play important roles in both normal and xenoestrogen disrupted testis physiology. however, the mechanisms and signaling pathways involved, in particular in fish, are largely unknown. we have used suppression subtractive hybridization to isolate 152 candidate estrogen-responsive genes in the testis of male estradiol (e2)-treated sea bream (sparus aurata). the e2 up-regulation of some of the genes (e.g., choriogenin l and h, vitellogenin i and ii, apolipopr ... | 2006 | 16267841 |
| life-history- and ecosystem-driven variation in composition and residence pattern of seabream species (perciformes: sparidae) in two mediterranean coastal lagoons. | species composition and length-frequency distributions of six sparid fish species were investigated in two central mediterranean coastal lagoons off the western coast of italy: fogliano and caprolace. in the former, the sparid fauna was dominated by the gilt-head seabream (sparus aurata), whereas in caprolace, species composition was more homogeneous across all six species. size structure varied considerably among species: s. aurata, diplodus puntazzo and diplodus vulgaris had a single-cohort st ... | 2006 | 16266729 |
| interactions of microorganisms isolated from gilthead sea bream, sparus aurata l., on vibrio harveyi, a pathogen of farmed senegalese sole, solea senegalensis (kaup). | four bacterial isolates from farmed gilthead sea bream, sparus aurata, included in a previous study as members of the vibrionaceae and pseudomonodaceae and the genus micrococcus, have been evaluated for their adhesive ability to skin and intestinal mucus of farmed senegalese sole, solea senegalensis, and their antagonistic effect on vibrio harveyi, a pathogen of sole. these isolates showed higher adhesion to sole mucus than the pathogenic strains of v. harveyi assayed. only two of the isolates s ... | 2005 | 16266326 |
| food-deprivation induces hsp70 and hsp90 protein expression in larval gilthead sea bream and rainbow trout. | heat shock proteins (hsps) expression is commonly used as indicators of cellular stress in animals. however, very little is known about either the expression patterns of hsps or their role in the stress-tolerance phenomenon in early life stages of fish. to this end, we examined the impact of food-deprivation (12 h), reduced oxygen levels (3.5 mg/l for 1 h) and heat shock (hs: +5 degrees c for 1 h) on hsp70 and hsp90 protein expression in early life stages of the gilthead sea bream (sparus aurata ... | 2005 | 16257553 |