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possible complication of bee stings and a review of the cardiac effects of bee stings.we report the case of a patient who, ∼3 weeks after multiple bee stings, developed a prolonged heart block, syncope and cardiac arrest. this required a temporary pacemaker to be implanted, which was later replaced with a permanent pacemaker. an ecg taken following surgery for a fractured humerus 6 years earlier was reportedly normal. the patient had been a rubber tapper who walked ∼1.5 km/day, but after the bee attack he was no longer able to walk or get up from the bed without experiencing sync ...201627803018
land-use change has no detectable effect on reproduction of a disturbance-adapted, hawkmoth-pollinated plant species.land-use change is cited as a primary driver of global biodiversity loss, with myriad consequences for species, populations, and ecosystems. however, few studies have examined its impact on species interactions, particularly pollination. furthermore, when the effects of land-use change on pollination have been studied, the focus has largely been on species pollinated by diurnal pollinators, namely, bees and butterflies. here, we focus on oenothera harringtonii, a night-flowering, disturbance-ada ...201627803000
a new marker based on the avian spindlin gene that is able to sex most birds, including species problematic to sex with chd markers.we have developed a new marker (z43b) that can be successfully used to identify the sex of most birds (69%), including species difficult or impossible to sex with other markers. we utilized the zebra finch taeniopygia guttata est microsatellite sequence (ck309496) which displays sequence homology to the 5' untranslated region (utr) of the avian spindlin gene. this gene is known to be present on the z and w chromosomes. to maximize cross-species utility, the primer set was designed from a consens ...201627801957
an introduction to the toxins special issue on "bee and wasp venoms: biological characteristics and therapeutic application".n/a.201627801836
antioxidant enzyme activities and lipid oxidation in rape (brassica campestris l.) bee pollen added to salami during processing.the present research investigated the antioxidant effect of rape (brassica campestris l.) bee pollen (rbp) on salami during processing. eight flavonoids in rbp ethanol extract were quantified by high-performance liquid chromatography-mass spectrometry (hplc-ms) analysis, and quercetin, rutin, and kaempferol were the major bioactive compounds. the rbp ethanol extract exhibited higher total antioxidant capacity than 6-hydroxy-2,5,7,8-tertramethylchromancarboxylic acid (trolox) at the same concentr ...201627801831
virus infections of honeybees apis mellifera.the health and vigour of honeybee colonies are threatened by numerous parasites (such as varroa destructor and nosema spp.) and pathogens, including viruses, bacteria, protozoa. among honeybee pathogens, viruses are one of the major threats to the health and well-being of honeybees and cause serious concern for researchers and beekeepers. to tone down the threats posed by these invasive organisms, a better understanding of bee viral infections will be of crucial importance in developing effectiv ...201527800411
dose-dependence of protection from systemic reactions to venom immunotherapy by omalizumab.systemic reactions (sr) to venom immunotherapy (vit) are rare but may occur, with a rate significantly higher for honeybee than for vespid vit. in patients with repeated srs to vit it is difficult to reach the maintenance dose of venom and pre-treatment with omalizumab is indicated, as shown by some studies reporting its preventative capacity, when antihistamines and corticosteroids are ineffective.201627799850
clinical impact of stomach-partitioning gastrojejunostomy with braun enteroenterostomy for patients with gastric outlet obstruction caused by unresectable gastric cancer.to compare adverse events and post-therapeutic clinical courses between stomach-partitioning gastrojejunostomy with braun enteroenterostomy (spgj-bee) and endoscopic metallic stent placement (emsp) in patients with gastric outlet obstruction (goo) caused by unresectable gastric cancer and assess the clinical utility of spgj-bee.201627798911
synthesis of enantiopure alicyclic ethers and their activity on the chemosensory organ of the ectoparasite of honey bees, varroa destructor.the preparation of enantiopure conformationally restricted alicyclic ethers and their inhibitory activities on the chemosensory organ of the varroa destructor, a parasite of honey bees, are reported in this article. we tested the effect of enantiopure ethers of cis-5-(2'-hydroxyethyl)cyclopent-2-en-1-ol on the varroa chemosensory organ by electrophysiology, for their ability to inhibit the responses to two honey bee-produced odors that are important for the mite to locate its host: nurse bee hea ...201627792333
reduction of dacarbazine cytogenetic effects on somatic cells in male mice using bee glue (propolis) to manifest the scientific miracles in the quran.this study was carried out to investigate the ability of propolis to ameliorate the adverse cytogenetic effects of dacarbazine on bone marrow cells.201627790359
[insect venom allergies : update 2016 for otorhinolaryngologists].due to the increasing incidence of hymenoptera venom allergies and the potentially life-threatening reactions, it is important for otolaryngologists working in allergology to have an understanding of modern diagnostic and treatment standards for this allergic disease. molecular diagnosis with recombinant single allergens from bee and wasp venom components improves the diagnostics of insect venom allergies, particularly in patients with double-positive extract-based test results. detection of spe ...201627787580
a novel screen for genes associated with pheromone-induced sterility.for honey bee and other social insect colonies the 'queen substance' regulates colony reproduction rendering workers functionally sterile. the evolution of worker reproductive altruism is explained by inclusive fitness theory, but little is known of the genes involved or how they regulate the phenotypic expression of altruism. we previously showed that application of honeybee queen pheromone to virgin fruit flies suppresses fecundity. here we exploit this finding to identify genes associated wit ...201627786267
a new technique for preserving the form of artificially inflated endophalli of bees.we present a simple technique for keeping the form of artificially expanded endophalli in bees (hymenoptera). endophalli were inflated using the introduction of low melting-point agarose from a syringe inserted in the anterior opening of the metasoma. under refrigeration, the endophalli kept their expanded shape for up to three days allowing the description of structure, morphometric analyses, and examination of the external sculpturing of the cuticle under scanning electron microscope. the tech ...201727785704
interaction of artepillin c with model membranes.green propolis, a mixture of beeswax and resinous compounds processed by apis mellifera, displays several pharmacological properties. artepillin c, the major compound in green propolis, consists of two prenylated groups bound to a phenyl group. several studies have focused on the therapeutic effects of artepillin c, but there is no evidence that it interacts with amphiphilic aggregates to mimic cell membranes. we have experimentally and computationally examined the interaction between artepillin ...201727785542
multiple sequence alignment using multi-objective based bacterial foraging optimization algorithm.multiple sequence alignment (msa) is a widespread approach in computational biology and bioinformatics. msa deals with how the sequences of nucleotides and amino acids are sequenced with possible alignment and minimum number of gaps between them, which directs to the functional, evolutionary and structural relationships among the sequences. still the computation of msa is a challenging task to provide an efficient accuracy and statistically significant results of alignments. in this work, the ba ...201627784624
differential proteomics reveals novel insights into nosema-honey bee interactions.host manipulation is a common strategy by parasites to reduce host defense responses, enhance development, host exploitation, reproduction and, ultimately, transmission success. as these parasitic modifications can reduce host fitness, increased selection pressure may result in reciprocal adaptations of the host. whereas the majority of studies on host manipulation have explored resistance against parasites (i.e. ability to prevent or limit an infection), data describing tolerance mechanisms (i. ...201627784614
evolution of resistance to single and combined floral phytochemicals by a bumble bee parasite.repeated exposure to inhibitory compounds can drive the evolution of resistance, which weakens chemical defence against antagonists. floral phytochemicals in nectar and pollen have antimicrobial properties that can ameliorate infection in pollinators, but evolved resistance among parasites could diminish the medicinal efficacy of phytochemicals. however, multicompound blends, which occur in nectar and pollen, present simultaneous chemical challenges that may slow resistance evolution. we assesse ...201727783434
mixtures of herbicides and metals affect the redox system of honey bees.the increasing loss of bee colonies in many countries has prompted a surge of studies on the factors affecting bee health. in north america, main crops such as maize and soybean are cultivated with extensive use of pesticides that may affect non-target organisms such as bees. also, biosolids, used as a soil amendment, represent additional sources of metals in agroecosystems; however, there is no information about how these metals could affect the bees. in previous studies we investigated the eff ...201727780120
preliminary investigation of mineral content of pollen collected from different serbian maize hybrids - is there any potential nutritional value?bee pollen has already proved to be a good supplement rich in iron and zinc. studies on the application of flower pollen in the food industry and medicine have begun. bearing in mind the prevalence of maize as a crop culture, its pollen will be easily available. the mineral composition of pollen of seven serbian maize hybrids was analyzed in order to establish its nutritional value and the benefits of its implementation in the human diet using the inductively coupled plasma method.201627778338
the proportion of impervious surfaces at the landscape scale structures wild bee assemblages in a densely populated region.given the predicted expansion of cities throughout the world, understanding the effect of urbanization on bee fauna is a major issue for the conservation of bees. the aim of this study was to understand how urbanization affects wild bee assemblages along a gradient of impervious surfaces and to determine the influence of landscape composition and floral resource availability on these assemblages. we chose 12 sites with a proportion of impervious surfaces (soil covered by parking, roads, and buil ...201627777733
genomic characterization of a fructophilic bee symbiont lactobacillus kunkeei reveals its niche-specific adaptation.lactobacillus kunkeei is classified as a sole obligate fructophilic lactic acid bacterium that is found in fructose-rich niches, including the guts of honeybees. the species is differentiated from other lactobacilli based on its poor growth with glucose, enhanced growth in the presence of oxygen and other electron acceptors, and production of high concentrations of acetate from the metabolism of glucose. these characteristics are similar to phylogenetically distant fructobacillus spp. in the pre ...201627776911
antennal transcriptome and differential expression analysis of five chemosensory gene families from the asian honeybee apis cerana cerana.chemosensory genes play a central role in sensing chemical signals and guiding insect behavior. the chinese honeybee, apis cerana cerana, is one of the most important insect species in china in terms of resource production, and providing high-quality products for human consumption, and also serves as an important pollinator. communication and foraging behavior of worker bees is likely linked to a complex chemosensory system. here, we used transcriptome sequencing on adult a. c. cerana workers of ...201627776190
microglomerular synaptic complexes in the sky-compass network of the honeybee connect parallel pathways from the anterior optic tubercle to the central complex.while the ability of honeybees to navigate relying on sky-compass information has been investigated in a large number of behavioral studies, the underlying neuronal system has so far received less attention. the sky-compass pathway has recently been described from its input region, the dorsal rim area (dra) of the compound eye, to the anterior optic tubercle (aotu). the aim of this study is to reveal the connection from the aotu to the central complex (cx). for this purpose, we investigated the ...201627774056
pollination biology of the hexaploid self-compatible species turnera velutina (passifloraceae).the evolution of monomorphisms from heterostylous ancestors has been related to the presence of homostyly and the loss of self-incompatibility, allowing the occurrence of selfing, which could be advantageous under pollinator limitation. however, flowers of some monomorphic species show herkogamy, attraction and rewarding traits that presumably favour cross-pollination and/or a mixed mating system. this study evaluated the contributions of pollinators, breeding system and floral traits to the rep ...201727770594
pollination of campomanesia phaea (myrtaceae) by night-active bees: a new nocturnal pollination system mediated by floral scent.bees are the most important diurnal pollinators of angiosperms. in several groups of bees a nocturnal/crepuscular habit developed, yet little is known about their role in pollination and whether some plants are adapted specifically to these bees. we used a multidisciplinary approach to investigate the reproductive biology and to understand the role of nocturnal/crepuscular bees in pollination of campomanesia phaea (myrtaceae), popularly named cambuci. we studied the floral biology and breeding s ...201727770587
seizure-specific wavelet (seizlet) design for epileptic seizure detection using correntropy ellipse features based on seizure modulus maximas patterns.eeg signal analysis of pediatric patients plays vital role for making a decision to intervene in presurgical stages.201727769876
mechanistic modeling of pesticide exposure: the missing keystone of honey bee toxicology.the role of pesticides in recent honey bee losses is controversial, partly because field studies often fail to detect effects predicted by laboratory studies. this dissonance highlights a critical gap in the field of honey bee toxicology: there exists little mechanistic understanding of the patterns and processes of exposure that link honey bees to pesticides in their environment. the authors submit that 2 key processes underlie honey bee pesticide exposure: 1) the acquisition of pesticide by fo ...201727769096
methoprene does not affect juvenile hormone titers in honey bee (apis mellifera) workers.methoprene, a juvenile hormone (jh) analog, is a widely used insecticide that also accelerates behavioral development in honey bees (apis mellifera). jh regulates the transition from nursing to foraging in adult worker bees, and treatment with jh or methoprene have both been shown to induce precocious foraging. to determine how methoprene changes honey bee behavior, we compared jh titers of methoprene-treated and untreated bees. behavioral observations confirmed that methoprene treatment signifi ...201627763722
trpa1 channels in drosophila and honey bee ectoparasitic mites share heat sensitivity and temperature-related physiological functions.the transient receptor potential cation channel, subfamily a, member 1 (trpa1) is conserved between many arthropods, and in some has been shown to function as a chemosensor for noxious compounds. activation of arthropod trpa1 channels by temperature fluctuations has been tested in only a few insect species, and all of them were shown to be activated by heat. the recent identification of chemosensitive trpa1 channels from two honey bee ectoparasitic mite species (vdtrpa1 and tmtrpa1) have provide ...201627761115
biological role of paenilarvins, iturin-like lipopeptide secondary metabolites produced by the honey bee pathogen paenibacillus larvae.the gram-positive bacterium paenibacillus larvae (p. larvae) is the causative agent of a deadly honey bee brood disease called american foulbrood (afb). afb is a notifiable epizootic in most countries and, hence, p. larvae is of considerable relevance for veterinarians and apiculturists alike. over the last decade, much progress has been made in the understanding of the (patho)biology of p. larvae. recently, several non-ribosomally produced peptides (nrp) and peptide/polyketide (nrp/pk) hybrids ...201627760211
estimating basal energy expenditure in liver transplant recipients: the value of the harris-benedict equation.reliable measurement of basal energy expenditure (bee) in liver transplant (lt) recipients is necessary for adapting energy requirements, improving nutritional status and preventing weight gain. indirect calorimetry (ic) is the gold standard for measuring bee. however, bee may be estimated through alternative methods, including electrical bioimpedance (bi), harris-benedict equation (hbe), and mifflin-st. jeor equation (msj) that carry easier applicability and lower cost.201727759783
body size phenology in a regional bee fauna: a temporal extension of bergmann's rule.bergmann's rule originally described a positive relationship between body size and latitude in warm-blooded animals. larger animals, with a smaller surface/volume ratio, are better enabled to conserve heat in cooler climates (thermoregulatory hypothesis). studies on endothermic vertebrates have provided support for bergmann's rule, whereas studies on ectotherms have yielded conflicting results. if the thermoregulatory hypothesis is correct, negative relationships between body size and temperatur ...201627758035
genotype diversity in the honey bee parasite nosema ceranae: multi-strain isolates, cryptic sex or both?there is great controversy as to whether microsporidia undergo a sexual cycle. in the paradigmatic case of nosema ceranae, although there is no morphological evidence of sex, some meiosis-specific genes are present in its reduced genome and there is also high intraspecific variability, with incongruent phylogenies having been systematically obtained. the possibility of sexual recombination is important from an epidemiological standpoint, particularly as n. ceranae is considered to be a major fac ...201627756211
season and landscape composition affect pollen foraging distances and habitat use of honey bees.honey bees (apis mellifera l.) show a large variation in foraging distances and use a broad range of plant species as pollen resources, even in regions with intensive agriculture. however, it is unknown how increasing areas of mass-flowering crops like oilseed rape (brassica napus; osr) or a decrease of seminatural habitats (snh) change the temporal and spatial availability of pollen resources for honey bee colonies, and thus foraging distances and frequency in different habitat types. we studie ...201627755712
hygienic behaviour in brazilian stingless bees.social insects have many defence mechanisms against pests and pathogens. one of these is hygienic behaviour, which has been studied in detail in the honey bee, apis mellifera hygienic honey bee workers remove dead and diseased larvae and pupae from sealed brood cells, thereby reducing disease transfer within the colony. stingless bees, meliponini, also rear broods in sealed cells. we investigated hygienic behaviour in three species of brazilian stingless bees (melipona scutellaris, scaptotrigona ...201627754850
metabolomic profiling of the effects of melittin on cisplatin resistant and cisplatin sensitive ovarian cancer cells using mass spectrometry and biolog microarray technology.in the present study, liquid chromatography-mass spectrometry (lc-ms) was employed to characterise the metabolic profiles of two human ovarian cancer cell lines a2780 (cisplatin-sensitive) and a2780cr (cisplatin-resistant) in response to their exposure to melittin, a cytotoxic peptide from bee venom. in addition, the metabolomics data were supported by application of biolog microarray technology to examine the utilisation of carbon sources by the two cell lines. data extraction with mzmine 2.14 ...201627754384
authors' response on hoppe et al. (2015) "effects of a neonicotinoid pesticide on honey bee colonies: a response to the field study by pilling et al. (2013)". environ sci eur (2015) 27-28.the published commentary by hoppe et al. (environ sci eur 27-28, 2015) makes a number of strong criticisms of pilling et al. (plos one 8:e77193, 2013), which this authors' response will show are either wrong, inaccurate or misleading. a selection of these misrepresentations include a claim that technical thiamethoxam was used rather than the commercial product. this is not true. pilling et al. (plos one 8:e77193, 2013) clearly state that formulated commercial products were used which also includ ...201527752432
effects of a neonicotinoid pesticide on honey bee colonies: a response to the field study by pilling et al. (2013).our assessment of the multi-year overwintering study by pilling et al. (2013) revealed a number of major deficiencies regarding the study design, the protocol and the evaluation of results. colonies were exposed for short periods per year to flowering oilseed rape and maize grown from thiamethoxam-coated seeds. thiamethoxam as the sole active ingredient was used, not a more efficacious commercial product, at seed treatment rates that were lower than recommended as per common agricultural practic ...201527752429
variations in lead, cadmium, arsenic, and mercury concentrations during honeybee wax processing using casting technology.beeswax foundations are a necessary material in intensive modern beekeeping. heavy metals can accumulate in these foundations for decades, as it is a common beekeeping practice to recycle wax. beeswax samples were analysed using gfaas for as, cd, pb, and hg concentrations during the production of beeswax foundations using casting technology with a prolonged cooling and sedimentation phase. significant differences were determined in the concentrations of as, cd, pb (p<0.01, all), and hg (p<0.05) ...201627749267
environment-specific modulation of odorant representations in the honeybee brain.ca(2+) imaging techniques were applied to investigate the neuronal behavior of projection neurons in the honeybee antennal lobe (al) to examine the effects of long-lasting adaptation on odorant coding. responses to eight test odorants were measured before, during, and after an odor adaptation phase. bees were exposed to the adapting odor for 30 min. test odorant responses were only recorded from a sub-population of accessible glomeruli on the al surface. projection neurons, the output neurons of ...201627748970
pair-rule patterning in the honeybee apis mellifera: expression of even-skipped combines traits known from beetles and fruitfly.we have studied the binding pattern of antibody mab 2b8 directed against even-skipped orthologous proteins (eve) in honeybee embryos. primary and secondary eve stripes form in roughly anterior-to-posterior succession; there are 8 primary and 16 secondary stripes. the most posterior primary stripes appear only after the onset of gastrulation. the secondary stripes form by a splitting of primary stripes; they demarcate the parasegmental pattern. while these findings resemble eve expression in long ...199727747387
acute extensive anterior st elevation myocardial infarction following bee sting: a rare report of kounis syndrome in lad territory.herein we report a case of extensive anterior myocardial infarction (mi) after bee sting, in 57-year-old man who had no known risk factors for coronary artery disease (cad).201627747172
brain transcriptomes of honey bees (apis mellifera) experimentally infected by two pathogens: black queen cell virus and nosema ceranae.regulation of gene expression in the brain plays an important role in behavioral plasticity and decision making in response to external stimuli. however, both can be severely affected by environmental factors, such as parasites and pathogens. in honey bees, the emergence and re-emergence of pathogens and potential for pathogen co-infection and interaction have been suggested as major components that significantly impaired social behavior and survival. to understand how the honey bee is affected ...201627747157
the circuitry of olfactory projection neurons in the brain of the honeybee, apis mellifera.in the honeybee brain, two prominent tracts - the medial and the lateral antennal lobe tract - project from the primary olfactory center, the antennal lobes (als), to the central brain, the mushroom bodies (mbs), and the protocerebral lobe (pl). intracellularly stained uniglomerular projection neurons were reconstructed, registered to the 3d honeybee standard brain atlas, and then used to derive the spatial properties and quantitative morphology of the neurons of both tracts. we evaluated putati ...201627746723
effects of emollient containing bee venom on atopic dermatitis: a double-blinded, randomized, base-controlled, multicenter study of 136 patients.atopic dermatitis (ad) is a common, complex disease that follows a chronic relapsing course and significantly affects the quality of life of patients. skin barrier dysfunction and inflammatory processes induce and aggravate this skin condition. proper use of an emollient for hydration is a keystone of ad treatment. bee venom is known to have anti-inflammatory effects and has been widely used in traditional medicine to treat various inflammatory disorders.201627746639
apis mellifera ligustica, spinola 1806 as bioindicator for detecting environmental contamination: a preliminary study of heavy metal pollution in trieste, italy.honeybees have become important tools for the ecotoxicological assessment of soil, water and air metal contamination due to their extraordinary capacity to bioaccumulate toxic metals from the environment. the level of heavy metal pollution in the trieste city was monitored using foraging bees of apis mellifera ligustica from hives owned by beekeepers in two sites strategically located in the suburban industrial area and urban ones chosen as control. the metal concentration in foraging bees was d ...201727744589
native bee diversity and pollen foraging specificity in cultivated highbush blueberry (ericaceae: vaccinium corymbosum) in rhode island.we identified 41 species of native bees from a total of 1,083 specimens collected at cultivated highbush blueberry plantings throughout rhode island in 2014 and 2015. andrena spp., bombus spp., and xylocopa virginica (l.) were collected most often. bombus griseocollis (degeer), b. impatiens cresson, b. bimaculatus cresson, b. perplexus cresson, and andrena vicina smith collected the largest mean numbers of blueberry pollen tetrads. the largest mean percent blueberry pollen loads were carried by ...201627744311
contribution of large-sized primary sensory neuronal sensitization to mechanical allodynia by upregulation of hyperpolarization-activated cyclic nucleotide gated channels via cyclooxygenase 1 cascade.under physiological state, small- and medium-sized dorsal root ganglia (drg) neurons are believed to mediate nociceptive behavioral responses to painful stimuli. however, recently it has been found that a number of large-sized neurons are also involved in nociceptive transmission under neuropathic conditions. nonetheless, the underlying mechanisms that large-sized drg neurons mediate nociception are poorly understood. in the present study, the role of large-sized neurons in bee venom (bv)-induce ...201727743933
food to some, poison to others - honeybee royal jelly and its growth inhibiting effect on european foulbrood bacteria.honeybee colonies (apis mellifera) serve as attractive hosts for a variety of pathogens providing optimal temperatures, humidity, and an abundance of food. thus, honeybees have to deal with pathogens throughout their lives and, even as larvae they are affected by severe brood diseases like the european foulbrood caused by melissococcus plutonius. accordingly, it is highly adaptive that larval food jelly contains antibiotic compounds. however, although food jelly is primarily consumed by bee larv ...201727743422
bumble bees regulate their intake of essential protein and lipid pollen macronutrients.bee population declines are linked to the reduction of nutritional resources due to land-use intensification, yet we know little about the specific nutritional needs of many bee species. pollen provides bees with their primary source of protein and lipids, but nutritional quality varies widely among host-plant species. therefore, bees might have adapted to assess resource quality and adjust their foraging behavior to balance nutrition from multiple food sources. we tested the ability of two bumb ...201627742891
[mortal poisoning by bee stings in a child: study of a case observed in the regional hospital of maradi (niger)].we report a fatal case due to a massive attack by a swarm of bees in a nine-year-old child. the accident was fatal because of two aggravating factors: the cephalic location and the large number of stings (about 300). complications were coagulopathy, anemia, hemorrhage, coma, and oligoanuria. support was symptomatic at a facility second level. the outcome was fatal within 14 days. in view of the encountered difficulties, we recommend to build written protocols for the management of envenomation i ...201627738911
comparative genomics of 9 novel paenibacillus larvae bacteriophages.american foulbrood disease, caused by the bacterium paenibacillus larvae, is one of the most destructive diseases of the honeybee, apis mellifera. our group recently published the sequences of 9 new phages with the ability to infect and lyse p. larvae. here, we characterize the genomes of these p. larvae phages, compare them to each other and to other sequenced p. larvae phages, and putatively identify protein function. the phage genomes are 38-45 kb in size and contain 68-86 genes, most of whic ...201727738559
nosema ceranae infection promotes proliferation of yeasts in honey bee intestines.nosema ceranae infection not only damages honey bee (apis melifera) intestines, but we believe it may also affect intestinal yeast development and its seasonal pattern. in order to check our hypothesis, infection intensity versus intestinal yeast colony forming units (cfu) both in field and cage experiments were studied.201627736915
improvement of neurological disorders in postmenopausal model rats by administration of royal jelly.royal jelly (rj) from honeybees (apis mellifera) has estrogenic activity. estrogen deficiency after menopause leads to a high risk of memory impairment and depression as well as metabolic syndrome and osteoporosis. we here investigated the effect of rj on memory impairment and depression-like behaviors in ovariectomized (ovx) rats.201627736245
monitoring of neonicotinoid pesticides in beekeeping.the decline of pollinating species is correlated to the extensive use of neonicotinoids against pest insects for crop protection. in this study, the concentrations of neonicotinoid insecticides were determined in honeybees, honeycomb and honey samples, collected in spring 2015 (blooming period) from different areas in sicily (it), to carry out an evaluation of bees products' safety and an overview of neonicotinoid contamination in beekeeping. the results obtained showed only the presence of clot ...201727736155
a fatal case caused by massive honey bee stings.bee venom is a complex substance which acts in different ways; local or systemic anaphylaxis associated with ige and direct toxic effects of the large volume of injected venom. we report a 10- year-old boy who was the vulnerable of 5989 honey bee attacks. to the best of our knowledge, this case had the ultimate number of honey bee stings in the literature, until now. he was admitted to pediatric intensive care unit after 3 hours of incident. plasmapheresis was started to remove circulating venom ...201727735801
is non-host pollen suitable for generalist bumblebees?current evidence suggests that pollen is both chemically and structurally protected. despite increasing interest in studying bee-flower networks, the constraints for bee development related to pollen nutritional content, toxicity and digestibility as well as their role in the shaping of bee-flower interactions have been poorly studied. in this study we combined bioassays of the generalist bee bombus terrestris on pollen of cirsium, trifolium, salix, and cistus genera with an assessment of nutrit ...201627731933
changes in the bacteriome of honey bees associated with the parasite varroa destructor, and pathogens nosema and lotmaria passim.the honey bee, apis mellifera, is a globally important species that suffers from a variety of pathogens and parasites. these parasites and pathogens may have sublethal effects on their bee hosts via an array of mechanisms, including through a change in symbiotic bacterial taxa. our aim was to assess the influence of four globally widespread parasites and pathogens on the honey bee bacteriome. we examined the effects of the ectoparasitic mite varroa destructor, the fungal pathogens nosema apis an ...201727730366
case of disseminated intravascular coagulation after honey bee sting. 201627728595
a rare manifestation of bee sting. 201627728582
hemiplegia following multiple bee sting. 201627727866
importance of ecological factors and colony handling for optimizing health status of apiaries in mediterranean ecosystems.we analyzed six apiaries in several natural environments with a mediterranean ecosystem in madrid, central spain, in order to understand how landscape and management characteristics may influence apiary health and bee production in the long term. we focused on five criteria (habitat quality, landscape heterogeneity, climate, management and health), as well as 30 subcriteria, and we used the analytic hierarchy process (ahp) to rank them according to relevance. habitat quality proved to have the h ...201627727312
phylogeography of partamona rustica (hymenoptera, apidae), an endemic stingless bee from the neotropical dry forest diagonal.the south america encompasses the highest levels of biodiversity found anywhere in the world and its rich biota is distributed among many different biogeographical regions. however, many regions of south america are still poorly studied, including its xeric environments, such as the threatened caatinga and cerrado phytogeographical domains. in particular, the effects of quaternary climatic events on the demography of endemic species from xeric habitats are poorly understood. the present study us ...201627723778
differential gene expression underlying ovarian phenotype determination in honey bee, apis mellifera l., caste development.adult honey bee queens and workers drastically differ in ovary size. this adult ovary phenotype difference becomes established during the final larval instar, when massive programmed cell death leads to the degeneration of 95-99% of the ovariole anlagen in workers. the higher juvenile hormone (jh) levels in queen larvae protect the ovaries against such degeneration. to gain insights into the molecular architecture underlying this divergence critical for adult caste fate and worker sterility, we ...201627720811
ties between ageing plasticity and reproductive physiology in honey bees (apis mellifera) reveal a positive relation between fecundity and longevity as consequence of advanced social evolution.honey bees (apis mellifera) are the best studied model of ageing among the social insects. as in other social insects, the reproductive queen far outlives her non-reproductive workers despite developing from the same genome in the same colony environment. thus, the different social roles of the two female castes are critical for the profound phenotypic plasticity. in several special cases, such as the reproductive workers of apis mellifera capensis, within-caste plasticity enables further studie ...201627720052
abiotic and biotic factors affecting the replication and pathogenicity of bee viruses.bees are important pollinators of plants in both agricultural and non-agricultural landscapes. recent losses of both managed and wild bee species have negative impacts on crop production and ecosystem diversity. therefore, in order to mitigate bee losses, it is important to identify the factors most responsible. multiple factors including pathogens, agrochemical exposure, lack of quality forage, and reduced habitat affect bee health. pathogen prevalence is one factor that has been associated wit ...201627720045
why do social insect queens live so long? approaches to unravel the sociality-aging puzzle.social insects are characterized by an apparent reshaping of the fecundity/longevity trade-off with sociality. currently, we have only sketchy information about the potential underlying causes and mechanisms of aging and senescence which in addition are restricted to few model insect organisms (mainly the fruit fly drosophila melanogaster and the honey bee apis mellifera). how can we gain a more thorough understanding how sociality shapes senescence and the fecundity/longevity trade-off? by revi ...201627720043
nutritional and mineral contents of honey extracted by centrifugation and pressed processes.in this study, wild honey samples extracted by two different methods (centrifugation and pressed processing) were characterized and compared based on their physicochemical, and nutritional properties, macro- and micro-mineral contents, and pollen counts. twelve colonies of africanized apis mellifera were used; six honey samples were obtained by centrifugation and six by honeycomb press. all physicochemical parameters of honey samples (moisture, ph, total acidity, ash, dry matter, and qualitative ...201727719904
beneficial microorganisms for honey bees: problems and progresses.nowadays, honey bees are stressed by a number of biotic and abiotic factors which may compromise to some extent the pollination service and the hive productivity. the eu ban of antibiotics as therapeutic agents against bee pathogens has stimulated the search for natural alternatives. the increasing knowledge on the composition and functions of the bee gut microbiota and the link between a balanced gut microbiota and health status have encouraged the research on the use of gut microorganisms to i ...201627717968
diet-related gut bacterial dysbiosis correlates with impaired development, increased mortality and nosema disease in the honeybee (apis mellifera).dysbiosis, defined as unhealthy shifts in bacterial community composition, can lower the colonization resistance of the gut to intrinsic pathogens. here, we determined the effect of diet age and type on the health and bacterial community composition of the honeybee (apis mellifera). we fed newly emerged bees fresh or aged diets, and then recorded host development and bacterial community composition from four distinct regions of the hosts' digestive tract. feeding fresh pollen or fresh substitute ...201627717118
compositional mapping of bitumen using local electrostatic force interactions in atomic force microscopy.in recent years, many researchers have investigated bitumen surface morphology, especially the so-called bee-like structures, in an attempt to relate the chemical composition and molecular conformation to bitumen micromechanics and ultimately performance properties. even though recent studies related surface morphology and its evolution to stiffness and stress localization, the complex chemical nature of bitumen and its time- and temperature-dependent properties still engender significant questi ...201727716931
molecular dynamics of the honey bee toxin tertiapin binding to kir3.2.tertiapin (tpn), a short peptide isolated from the venom of the honey bee, is a potent and selective blocker of the inward rectifier k(+) (kir) channel kir3.2. here we examine in atomic detail the binding mode of tpn to kir3.2 using molecular dynamics, and deduce the key residue in kir3.2 responsible for tpn selectivity. the binding of tpn to kir3.2 is stable when the side chain of either lys16 (tpn(k16)-kir3.2) or lys17 (tpn(k17)-kir3.2) of the toxin protrudes into the channel pore. however, th ...201627716538
production of knockout mutants by crispr/cas9 in the european honeybee, apis mellifera l.the european honeybee (apis mellifera l.) is used as a model organism in studies of the molecular and neural mechanisms underlying social behaviors and/or advanced brain functions. the entire honeybee genome has been sequenced, which has further advanced molecular biologic studies of the honeybee. functions of genes of interest, however, remain largely to be elucidated in the honeybee due to the lack of effective reverse genetic methods. moreover, genetically modified honeybees must be maintaine ...201627715425
ingestion of bt rice pollen does not reduce the survival or hypopharyngeal gland development of apis mellifera adults.because of its ecological and economic importance, the honey bee apis mellifera is commonly used to assess the environmental risk of insect-resistant, genetically modified plants. in the current laboratory study, feeding-exposure experiments were used to determine whether pollen from transgenic rice harms a. mellifera worker bee. in one experiment, the survival and mean acinus diameter of hypopharyngeal glands of adult bees were similar when bees were fed on pollen from bt rice lines or from a n ...201627714836
moku virus; a new iflavirus found in wasps, honey bees and varroa.there is an increasing global trend of emerging infectious diseases (eids) affecting a wide range of species, including honey bees. the global epidemic of the single stranded rna deformed wing virus (dwv), driven by the spread of varroa destructor has been well documented. however, dwv is just one of many insect rna viruses which infect a wide range of hosts. here we report the full genome sequence of a novel iflavirus named moku virus (mv), discovered in the social wasp vespula pensylvanica col ...201627713534
review of field and monitoring studies investigating the role of nitro-substituted neonicotinoid insecticides in the reported losses of honey bee colonies (apis mellifera).the nitro-substituted neonicotinoid insecticides, which include imidacloprid, thiamethoxam and clothianidin, are widely used to control a range of important agricultural pests both by foliar applications and also as seed dressings and by soil application. since they exhibit systemic properties, exposure of bees may occur as a result of residues present in the nectar and/or pollen of seed- or soil-treated crop plants and so they have been the subject of much debate about whether they cause advers ...201627709399
beyond 16s rrna community profiling: intra-species diversity in the gut microbiota.interactions with microbes affect many aspects of animal biology, including immune system development, nutrition and health. in vertebrates, the gut microbiota is dominated by a small subset of phyla, but the species composition within these phyla is typically not conserved. moreover, several recent studies have shown that bacterial species in the gut are composed of a multitude of strains, which frequently co-exist in their host, and may be host-specific. however, since the study of intra-speci ...201627708630
bee happy. 201627708091
effects of varroa destructor on temperature and humidity conditions and expression of energy metabolism genes in infested honeybee colonies.varroa destructor mites pose an increasing global threat to the apicultural industry and agricultural ecology; however, the issue of whether certain environmental factors reflect the level of mite infection is far from resolved. here, a wireless sensor network (wsn) system was used to examine how v. destructor, which has vital impacts on honeybee (apis mellifera) health and survival, affects the temperature and humidity of honeybee hives in a field experiment. this approach may facilitate early ...201627706787
management-related traffic as a stressor eliciting parental care in a roadside-nesting bird: the european bee-eater merops apiaster.traffic is often acknowledged as a threat to biodiversity, but its effects have been mostly studied on roads subjected to high traffic intensity. the impact of lower traffic intensity such as those affecting protected areas is generally neglected, but conservation-oriented activities entailing motorized traffic could paradoxically transform suitable habitats into ecological traps. here we questioned whether roadside-nesting bee-eaters merops apiaster perceived low traffic intensity as a stressor ...201627706229
predicting acute contact toxicity of pesticides in honeybees (apis mellifera) through a k-nearest neighbor model.ecological risk assessment of plant protection products (ppps) requires an understanding of both the toxicity and the extent of exposure to assess risks for a range of taxa of ecological importance including target and non-target species. non-target species such as honey bees (apis mellifera), solitary bees and bumble bees are of utmost importance because of their vital ecological services as pollinators of wild plants and crops. to improve risk assessment of ppps in bee species, computational m ...201727705831
contextual organismality: beyond pattern to process in the emergence of organisms.biologists have taken the concept of organism largely for granted. however, advances in the study of chimerism, symbiosis, bacterial-eukaryote associations, and microbial behavior have prompted a redefinition of organisms as biological entities exhibiting low conflict and high cooperation among their parts. this expanded view identifies organisms in evolutionary time. however, the ecological processes, mechanisms, and traits that drive the formation of organisms remain poorly understood. recogni ...201627704542
lateralization of gene expression in the honeybee brain during olfactory learning.in the last decade, it has been demonstrated that brain functional asymmetry occurs not only in vertebrates but also in invertebrates. however, the mechanisms underlying functional asymmetry remain unclear. in the present study, we trained honeybees of the same parentage and age, on the proboscis extension reflex (per) paradigm with only one antenna in use. the comparisons of gene expression between the left and right hemispheres were carried out using high throughput sequencing. our research re ...201627703214
a mathematical model for the interplay of nosema infection and forager losses in honey bee colonies.we present a mathematical model (a) for the infection of a honey bee colony with nosema ceranae. this is a system of five ordinary differential equations for the dependent variables healthy and infected worker bees in the hive, healthy and infected forager bees, and disease potential deposited in the hive. the model is then (b) extended to account for increased forager losses, e.g. caused by exposure to external stressors. the model is non-autonomous with periodic coefficient functions. algebrai ...201627702226
the effect of seasons on brazilian red propolis and its botanical source: chemical composition and antibacterial activity.the aim of this study was to evaluate the effect of seasons on the chemical composition and antibacterial activity of brazilian red propolis (brp) and its plant source. brp was collected from maceio, alagoas state, north-east of brazil, during one year. chemical composition was determined by physicochemical analyses and hplc while antimicrobial activity was assessed against streptococcus mutans, streptococcus sobrinus, staphylococcus aureus and actinomyces naeslundii by determining the minimal i ...201727701899
the glass is not yet half empty: agitation but not varroa treatment causes cognitive bias in honey bees.honey bees (apis mellifera) are prone to judge an ambiguous stimulus negatively if they had been agitated through shaking which simulates a predator attack. such a cognitive bias has been suggested to reflect an internal emotional state analogous to humans who judge more pessimistically when they do not feel well. in order to test cognitive bias experimentally, an animal is conditioned to respond to two different stimuli, where one is punished while the other is rewarded. subsequently a third, a ...201727699501
bee venom (apis mellifera) an effective potential alternative to gentamicin for specific bacteria strains: bee venom an effective potential for bacteria.mellitine, a major component of bee venom (bv, apis mellifera), is more active against gram positive than gram negative bacteria. moreover, bv has been reported to have multiple effects, including antibacterial, antivirus, and anti-inflammation effects, in various types of cells. in addition, wasp venom has been reported to have antibacterial properties. the aim of this study was to evaluate the antibacterial activity of bv against selected gram positive and gram negative bacterial strains of me ...201627695631
insect visitors and potential pollinators of orchis militaris (orchidaceae) in southern belgium.as part of a research project on the food deception strategy in orchis militaris (l.), the objective of this study was to identify insect visitors and potential pollinators of this orchid species in belgium. in 2013, insects were collected over 2 d per site in five localities distributed in southern belgium (wallonia). a total of 104 insects belonging to 49 species were caught. dipterans were the most abundant visitors (50% of total specimens), followed by hymenopterans (32%). rhingia campestris ...201627694346
melittin ameliorates cvb3-induced myocarditis via activation of the hdac2-mediated gsk-3β/nrf2/are signaling pathway.viral myocarditis (vmc) is characterized as an inflammatory process of the myocardium and can be fatal in infants and children. melittin is a major polypeptide in honey bee venom that has been traditionally used against inflammation. however, its effect on vmc and the underlying molecular mechanism has not been reported. in this study, balb/c mice were intraperitoneally injected with cvb3 to build a vmc model and treated with melittin. the results showed that melittin increased the mice's body w ...201627693786
viability and infectivity of fresh and cryopreserved nosema ceranae spores.the microsporidium fungus nosema ceranae is an intracellular parasite that infects the midgut of the honey bee, apis mellifera. a major limitation of research on n. ceranae is that the fungus is non-culturable and thus studying it depends on the seasonal availability of nosema spores. also, spore viability and infectivity can vary considerably, and thus there is a need for reliable methods for determining those traits. this study examined different conditions for n. ceranae spore cryopreservatio ...201627693753
honeybees learn landscape features during exploratory orientation flights.exploration is an elementary and fundamental form of learning about the structure of the world [1-3]. little is known about what exactly is learned when an animal seeks to become familiar with the environment. navigating animals explore the environment for safe return to an important place (e.g., a nest site) and to travel between places [4]. flying central-place foragers like honeybees (apis mellifera) extend their exploration into distances from which the features of the nest cannot be directl ...201627693138
traceability of satsuma mandarin (citrus unshiu marc.) honey through nectar/honey-sac/honey pathways of the headspace, volatiles, and semi-volatiles: chemical markers.headspace solid-phase microextraction (hs-spme) and ultrasonic solvent extraction (use), followed by gc-ms/fid, were applied for monitoring the nectar (ne)/honey-sac (hos)/honey (ho) pathways of the headspace, volatiles, and semi-volatiles. the major ne (4 varieties of citrus unshiu) headspace compounds were linalool, α-terpineol, 1h-indole, methyl anthranilate, and phenylacetonitrile. corresponding extracts contained, among others, 1h-indole, methyl anthranilate, 1,3-dihydro-2h-indol-2-one and ...201627689986
bee venom ameliorates cognitive dysfunction caused by neuroinflammation in an animal model of vascular dementia.vascular dementia (vad) is caused by the reduction of blood supply by vessel occlusion and is characterized by progressive cognitive decline. vad incidence has been growing due to the aging population, placing greater strain on social and economic resources. however, the pathological mechanisms underlying vad remain unclear. many studies have used the bilateral common carotid artery occlusion (bccao) animal model to investigate potential therapeutics for vad. in this study, we investigated wheth ...201627686075
interspecific sensitivity of bees towards dimethoate and implications for environmental risk assessment.wild and domesticated bee species are exposed to a variety of pesticides which may drive pollinator decline. due to wild bee sensitivity data shortage, it is unclear if the honey bee apis mellifera is a suitable surrogate species in the current eu risk assessment scheme. furthermore, the underlying causes for sensitivity differences in bees are not established. we assessed the acute toxicity (median lethal dose, ld50) of dimethoate towards multiple bee species, generated a species sensitivity di ...201627686060
molecular diagnosis and immunotherapy.to describe recent insights into how molecular diagnosis can improve indication and selection of suitable allergens for specific immunotherapy and increase the safety of this therapy.201627685663
synergistic mortality between a neonicotinoid insecticide and an ergosterol-biosynthesis-inhibiting fungicide in three bee species.neonicotinoid insecticides have been identified as an important factor contributing to bee diversity declines. nonetheless, uncertainties remain about their impact under field conditions. most studies have been conducted on apis mellifera and tested single compounds. however, in agricultural environments, bees are often exposed to multiple pesticides. we explore the synergistic mortality between a neonicotinoid (clothianidin) and an ergosterol-biosynthesis-inhibiting fungicide (propiconazole) in ...201627685544
a push-pull protocol to reduce colonization of bird nest boxes by honey bees.introduction of the invasive africanized honey bee (ahb) into the neotropics is a serious problem for many cavity nesting birds, specifically parrots. these bees select cavities that are suitable nest sites for birds, resulting in competition. the difficulty of removing bees and their defensive behavior makes a prevention protocol necessary. here, we describe a push-pull integrated pest management protocol to deter bees from inhabiting bird boxes by applying a bird safe insecticide, permethrin, ...201627685258
preparation of single-cohort colonies and hormone treatment of worker honeybees to analyze physiology associated with role and/or endocrine system.honeybee workers are engaged in various tasks related to maintaining colony activity. the tasks of the workers change according to their age (age-related division of labor). young workers are engaged in nursing the brood (nurse bees), while older workers are engaged in foraging for nectar and pollen (foragers). the physiology of the workers changes in association with this role shift. for example, the main function of the hypopharyngeal glands (hpgs) changes from the secretion of major royal jel ...201627684286
high species richness of native pollinators in brazilian tomato crops.pollinators provide an essential service to natural ecosystems and agriculture. in tomatoes flowers, anthers are poricidal, pollen may drop from their pore when flowers are shaken by the wind. however, bees that vibrate these anthers increase pollen load on the stigma and in fruit production. the present study aimed to identify the pollinator richness of tomato flowers and investigate their morphological and functional traits related to the plant-pollinator interaction in plantations of central ...201627683812
association of environmental markers with childhood type 1 diabetes mellitus revealed by a long questionnaire on early life exposures and lifestyle in a case-control study.the incidence of childhood type 1 diabetes (t1d) incidence is rising in many countries, supposedly because of changing environmental factors, which are yet largely unknown. the purpose of the study was to unravel environmental markers associated with t1d.201627682602
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