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simple nonradioactive dna hybridization method for identification of sibling species of anopheles dirus (diptera: culicidae) complex.a simple method was developed for species identification of mosquitoes in the anopheles dirus complex found in thailand using horseradish peroxidase-labeled dna probes and a chemiluminescent detection system. species-specific dna probes for anopheles dirus, species b, c, and d detected 1-5 ng of target dna, a sensitivity that was comparable with the radioisotopic detection system. identification of individual mosquitoes was performed by dot-blot analysis of crude mosquito dna. the method allowed ...19957608914
vector control in some countries of southeast asia: comparing the vectors and the strategies.the use of information on malaria vector behaviour in vector control is discussed in relation to the area of southeast asia comprising cambodia, laos, myanmar, thailand and vietnam. the major vectors in the region are anopheles dirus, an. minimus, an. maculatus and an. sundaicus, of which an. dirus is the most important. options for vector control and the biological features of mosquitoes, which would make them amenable to control by these measures, are listed. the methods with the greatest pote ...19957605123
blood volume ingested by anopheles dirus using membrane feeding. 19817256350
development of plasmodium inui in the aotus trivirgatus monkey.four splenectomized aotus trivirgatus griseimembra monkeys were affected with the os strain of plasmodium inui, a parasite of old world macaques. high parasite densities were obtained and two of the animals died. surviving animals maintained low to moderate level of chronic infections. three of the four animals produced parasitemias which infected anopheles dirus mosquitoes; oocyst densities were very low, however.19817241282
forest malaria in bangladesh. iii. breeding habits of anopheles dirus.a thorough, repetitive, 21-month search for breeding sites of anopheles dirus (=an. balabacensis s.l.) was conducted within a 3-km radius of an isolated, holoendemic malaria focus. during the annual 6-month monsoon, all breeding within 1.5 km of the focus took place at eight sites in 1975 and at nine sites in 1976. these sites were of two topographic types--puddles on footpaths and turbulence pits at the heads of drainage gullies--that shared the characteristic of being able to hold water for at ...19827072884
forest malaria in bangladesh. ii. transmission by anopheles dirus.seasonal, holoendemic malaria transmission in a small, isolated forest community was studied by doing outdoor and indoor all-night man-biting catches over 21 consecutive months. more than 3.8% of anopheles dirus (=an. balabacensis s.l.), the most frequently caught anopheline, were infective. one an. annularis was also infective. transmission occurred only during the 7-month monsoon. in the absence of ddt, an. dirus bit with equal frequency indoors and outdoors. when ddt was present in dwellings, ...19827072883
sporozoite-induced plasmodium cynomolgi infections in captive born macaca fascicularis.capability of captive born cynomolgus monkeys to substitute for rhesus in the plasmodium cynomolgi radical curative antimalarial drug development model was examined. eighteen monkeys divided into 3 groups were given standard or high doses of sporozoites intravenously. one group of 4 received 0.8 - 1.6 x 10(6) and a second group of 8 received 0.3 - 1.0 x 10(7) sporozoites. the third group of 6 was splenectomized and then received 3.0 - 4.0 x 10(6). the 2 groups of intact monkeys developed a persi ...19846740373
esterase patterns of anopheles dirus (bangkok strain) and anopheles balabacensis (perlis form) in the laboratory.esterase isoenzyme were performed in anopheles balabacensis (perlis form) and anopheles dirus (bangkok strain and kanchanaburi strain). the zymogram showed four positions, e1, e2, e3 and e4. seventy-five percent of an. balabacensis (perlis form) or an. dirus (bangkok strain) can be correctly diagnosed by the presence of fast band in e3 or super slow band in e3 respectively. homozygous slow band of e3 cannot be fully distinguished in both species, but by the combination of e2 and e3, 99.42% can b ...19836612417
cytogenetic studies of some species complexes of anopheles in thailand and southeast asia.recent studies on cytogenetics, behavioral, geographical and distinct morphological characters on adult, pupal and larval stages have revealed that "balabacensis" is a species complex. anopheles dirus the mainland species, is distributed widely in thailand and is renowned for its role as primary vector of human malarial parasites. further, evidence from cytogenetic and taxonomic studies suggests that "an. dirus" is a species complex comprising at least four distinct species provisionally designa ...19846543543
infection and transmission studies with the n-3 strain of plasmodium fieldi in the macaca mulatta monkey.seven different anophelines--anopheles freeborni, an. dirus, an. maculatus, an. atroparvus, an. stephensi, an. albimanus, and an. quadrimaculatus--were shown to be susceptible to infection with the n-3 strain of plasmodium fieldi. transmission was obtained via the bites of an. dirus, an. stephensi, and an. maculatus mosquitoes to macaca mulatta monkeys. sporozoites dissected from an. freeborni were also shown to be infectious. anopheles dirus and an. stephensi were the most suitable mosquitoes f ...19846491849
plasmodium falciparum infection in bisazir chemosterilized anopheles dirus in laboratory studies.bisazir, at a 0.5% solution induced sterility in males and at a 1.5% solution in female a. dirus. these sterilizing doses reduced p. falciparum infection in mosquitoes, however, they can still transmit malaria. it is concluded that by the concentration of 1.5 and 2.0% that induced complete sterility in males and females are not safe in sterile-male release programme for the control of a. dirus, unless all females were eliminated prior to release.19846390705
[experimental study on susceptibility of anopheles minimus and anopheles dirus to plasmodium vivax and plasmodium falciparum]. 19836380800
studies on a newly isolated strain of plasmodium brasilianum in aotus and saimiri monkeys and different anophelines.a strain of plasmodium brasilianum was isolated from an aotus vociferans monkey from peru. the parasite readily infected aotus monkeys from bolivia and columbia and saimiri sciureus monkeys from peru and bolivia. highest level mosquito infections were obtained by feeding on the saimiri monkeys. the most susceptible mosquito was anopheles freeborni, followed by anopheles dirus, anopheles stephensi, anopheles gambiae, anopheles culicifacies, anopheles maculatus and anopheles albimanus. anopheles q ...19854093810
[observation on the susceptibility of anopheles dirus to plasmodium yoelii yoelii]. 19854053316
[experimental observation on the dispersal of anopheles dirus in hainan island]. 19854053315
the sporogonic cycle of plasmodium reichenowi.plasmodium reichenowi, a malarial parasite of the chimpanzee, was infective to anopheles freeborni, anopheles quadrimaculatus, anopheles stephensi, anopheles maculatus, anopheles dirus, and anopheles culicifacies mosquitoes. anopheles gambiae and anopheles albimanus were not infected. mean oocyst diameters of p. reichenowi were smaller than those of the other chimpanzee parasite, plasmodium schwetzi. sporozoites were present in the salivary glands of an. freeborni at 15 days when held at 25 to 2 ...19863734994
intraspecific variation in sex heterochromatin of species b of the anopheles dirus complex in thailand.cytological examination of f1 larval mitotic chromosomes from a total of 126 families of anopheles dirus species b from southern thailand populations has revealed a pronounced quantitative variation of constitutive heterochromatin in the two sex chromosomes. five types of x chromosomes and four types of y chromosomes have been identified in this study. such gross variation in sex chromosomes is most likely due to a gradual acquisition of extra heterochromatin.19873609738
monandry (monogamy) in natural populations of anopheline mosquitoes.polymorphic y chromosomes within two species of anopheline mosquitoes provide markers for testing if females are inseminated by one or more males in nature. wild-caught females of anopheles dirus (sp. a) and an. maculatus s.s. produced 291 and 55 families, respectively, which showed a single type of y chromosome. one family of the former species showed two types of y chromosomes. these field data support the idea, established from laboratory studies, that female mosquitoes are largely monandrous ...19873504933
correlation of survival rates of anopheles dirus a (diptera: culicidae) with different infection densities of plasmodium cynomolgi.the survival rates are described for 36 paired replicates of anopheles dirus a mosquitos that had been allowed to engorge themselves on rhesus monkeys that were either infected or non-infected with plasmodium cynomolgi. the survival rates of infected mosquitos with a mean number of oocysts less than 10 did not differ significantly from those that were non-infected; however, there was a significant difference in the survival rates of non-infected groups and those with a mean number of oocysts in ...19863493859
scanning electron microscopic study of pupal seta 9-111-v of anopheles balabacensis (perlis form) and anopheles dirus (bangkok strain). 19873448784
geographic distribution and biting behaviour of four species of the anopheles dirus complex (diptera: culicidae) in thailand.a cytogenetic analysis of the species status of members of the anopheles dirus group, from natural populations, over a six-year period has produced biogeographic and behavioural data which are presented herein. species a, b, c and d have quite distinct geographic distributions in thailand. species a is the only species in most of the mainland but is absent from the southern half of the peninsula. species b is dominant in the far south of the peninsula giving way to species c on the north-east si ...19883406803
transmission of the os strain of plasmodium inui to saimiri sciureus boliviensis and aotus azarae boliviensis monkeys by anopheles dirus mosquitoes.eight saimiri and 7 aotus monkeys were exposed to infection with the os strain of plasmodium inui via the bites of from 2 to 7 anopheles dirus mosquitoes. all saimiri monkeys developed high-level infections of from 152,000 to 500,000/mm3 after prepatent periods of from 14 to 17 days. only 1 aotus monkey developed a patent infection after a period of 28 days. feeding on these animals failed to result in infection of an. dirus mosquitoes.19883379532
identification of isomorphic malaria vectors using a dna probe.about 7,000 recombinant clones, derived from chromosomally-identified families of wild-caught females of anopheles dirus species d, were screened. the most promising clone was totally specific to species d when tested against single f1 females of all four species of the complex. in fresh specimens the clone was positive for dna levels 150 times less than the normal dna content of single individuals. fresh adult males and females, larvae, and dried specimens have been successfully identified. the ...19883341521
some entomological observations on malaria transmission in a remote village in northwestern thailand.anopheline mosquitoes and their relation to malaria transmission were studied during the months of march, may and july in the karen village of mae tha waw located in the northwestern mountains of tak province. thirteen species were captured on human bait during 80 man-nights of collecting. four additional species were collected during routine larval surveys. anopheles minimus and an. maculatus comprised 92.5% of the specimens captured biting man. anopheles minimus and an. nivipes were implicated ...19873333058
a note on anopheles dirus peyton and harrison, 1979 [an. balabacensis (sensu lato) baisas, 1936] in india. 19883268459
diagnostic restriction fragment patterns of dna from the four isomorphic species of anopheles dirus.total dna from isofemale lines of the four isomorphic species of the anopheles dirus were screened against twenty restriction endonucleases. seven enzymes (ava ii, alu i, bgl ii, hae iii, hinf i, mbo i and sau3a i) produced some unique dna fragments for each of the lines. seven other enzymes (bamh i, bstn i, cfo i, ecor i, kpn i, nru i and pst i) produced unique fragments in two of the lines. the remaining six enzymes (hha i, mnl i, msp i, nae i, rsa i and taq i) gave vague patterns which might ...19883238483
distribution and chromosomal polymorphism of the malaria vector anopheles dirus species d.preliminary results indicate that species d of the anopheles dirus complex is widespread west of the thai-burma border in burma and bangladesh. a chromosomal study of an. dirus species d in these areas has revealed that this malaria vector is highly polymorphic for chromosomal rearrangements in salivary gland polytene chromosomes. the data from the limited number of wild-caught samples suggest that different geographically isolated populations may occur with respect to the frequency of inversion ...19883238479
cytogenetic evidence for a fifth species within the taxon anopheles dirus in thailand.crossbreeding and chromosomal evidence are presented for the existence of a fifth sibling species within the taxon of anopheles dirus in thailand. the new species is morphologically identifiable as anopheles balabacensis "fraser's hill form." structural differences in mitotic chromosomes and extensive asynapsis in hybrid polytene chromosomes indicate that significant genetic divergence exists between this species and its closest relatives, an. dirus species a, b, c and d and an. balabacensis.19883199123
cytological differences and chromosomal rearrangements in four members of the anopheles dirus complex (diptera: culicidae).a reference photomap of the larval salivary gland, polytene chromosomes of the anopheles dirus complex (species a) is presented. samples of species a, b, c, and d from natural populations in thailand were compared to this standard map using the larval progeny of wild-caught females. all species show differences in their chromosome banding patterns involving band size, number, and shape, particularly at the free ends of the x, 2r, and 2l. these differences provide useful diagnostic characters for ...19883169545
establishment of a natural mating colony of anopheles dirus peyton et harrison and their biological characteristics. 19873113847
effects of cyromazin and methoprene on the developmental stages of anopheles dirus, aedes aegypti and culex quinquefasciatus (diptera : culicidae).the effects of two chemical compounds, cyromazin and methoprene, on the developmental stages of anopheles dirus, aedes aegypti and culex quinquefasciatus were investigated under laboratory conditions, with the mean temperature of 24 degrees +/- 1 degree c and the relative humidity at 65-75%. both compounds were tested against the second, third and fourth instar larvae. the concentrations of cyromazin used for an. dirus and cx. quinquefasciatus ranged from 0.0008 to 0.5 mg/l; and for ae. aegypti ...19852866585
the importance of anopheles dirus (a. balabacensis) as a vector of malaria in northeast india.anopheles dirus (a. balabacensis) is the most prevalent among twelve anopheline species collected during the course of entomological studies carried out in changlang district of arunachal pradesh during july and august, 1988. it is observed to be a forest species and is found to breed in small stagnant water pools completely or partially shaded by plants in the open jungle. sporozoites have been detected in several specimens of a. dirus (a. balabacensis) supporting its role in transmission of ma ...19892792474
incrimination of anopheles dirus as a vector of malaria in dibrugarh district, assam.anopheles nigerrimus, a. vagus, a. dirus, a. karwari and a. kochi were the most abundant species among 12 anopheline species collected during the course of entomological studies carried out from july to september, 1988 in an area endemic for malaria under tengakhat phc of dibrugarh district, assam. sporozoites were detected in the salivary glands of one specimen of a. dirus, peyton & harrison (a. balabacensis) which constituted about 18.11 per cent of the total anopheline collection.19892699591
[observation on the development of plasmodium falciparum in anopheles dirus].this paper is a record of our observation on the stages of development of plasmodium falciparum in anopheles dirus. the malarial parasites were derived from 5 infected patients living in guizhou province and used to infect 8 batches of an.dirus. the morphology of various developmental stages studied under light microscope and their average size were as follows: microgametes were filament-shaped, 13.31 +/- 2.22 microns; macrogametes and zygotes oval or round, 4.36 +/- 0.59 microns and 3.39 +/- 0. ...19892699280
scanning electron microscopic observations and differentiation of eggs of the anopheles dirus complex.microscopic observations have revealed differences among the eggs of species a, b, c and d of the anopheles dirus complex. the eggs of species a and c are similar in size and shape. they are intermediate in size between the eggs of species b, which is the largest, and that of species d, which is the smallest. the pattern of outer chorionic cells between the frill and the float is species specific. the pattern consists of rows of irregularly shaped cells in species d and different numbers of rows ...19892614407
[comparison in susceptibility of anopheles dirus and anopheles stephensi to b strain of plasmodium cynomolgi].this paper reports on the comparative susceptibility of a. dirus (hainan strain) and a. stephensi (hor strain) to the b strain of p. cynomolgi in paired feeding experiments. in the most susceptible infective period, the infection rate in midgut and salivary gland of the two species was over 90%, the difference is not statistically significant. in relatively infective period, three experiments were performed, the infection rate in midgut of a. dirus was 24.4-60.4% with an average of 46.5% (53/114 ...19892591037
a nitrocellulose membrane-based elisa for the detection of plasmodium infections in mosquitos.a nitrocellulose (nc) membrane was evaluated as a solid-phase support for the detection of malaria-infected mosquitos using monoclonal antibodies (mab) with a laboratory model based on plasmodium inui and anopheles dirus. mabs produced against sporozoites of the n34 strain of p. inui, and selected by immunofluorescence assay and the circumsporozoite precipitin test, were used. a one-site indirect nc-elisa that used unlabelled mab and enzyme-labelled anti-mouse igg was developed. its sensitivity ...19892575463
hyperendemic malaria in a thai village: dependence of year-round transmission on focal and seasonally circumscribed mosquito (diptera: culicidae) habitats.in a longitudinal study of hyperendemic malaria in a village in eastern thailand (from october 1985 to november 1987), man-biting anopheline mosquitoes were collected for 16 man-nights per month in 22 of 26 mo. mosquitoes were separated according to collection sites (inner, central, more populated; outer, peripheral, more forested), biting period, and parity, and then they were tested for sporozoite antigen using an enzyme-linked immunosorbent assay (elisa). abundance of anopheles dirus peyton & ...19902280385
evaluation of survival potential and malaria susceptibility among different size classes of laboratory-reared anopheles dirus.four size classes of anopheles dirus were reared from different larval densities. higher densities produced smaller adults with lower survivorship. larger females took larger bloodmeals by artificial feeding with cultured plasmodium falciparum and developed significantly more oocysts.19902240360
anopheles dirus species e: chromosomal and crossing evidence for another member of the dirus complex.cytogenetic and crossing data provide strong evidence for the existence of another species, dirus e in southwestern india, within the dirus complex of anopheles. these findings are in accord with unpublished morphological observations. our data suggest a significant genetic divergence between species e and its close relatives, an. dirus a, b and c in thailand. these data also suggest that dirus e is an incipient sibling species of its geographically nearest relative, dirus d, and that it seeming ...19902230776
the peruvian iii strain of plasmodium brasilianum in saimiri sciureus boliviensis monkeys.a strain of plasmodium brasilianum was isolated from a naturally infected saimiri monkey from peru and subsequently passaged to 21 splenectomized saimiri sciureus boliviensis monkeys. nine of 12 attempts to transmit infection by sporozoite inoculation were successful with prepatent periods ranging from 23 to 41 days. gametocytes were infective to anopheles freeborni, anopheles stephensi, anopheles dirus, anopheles maculatus, and anopheles gambiae mosquitoes. the strain demonstrated a high level ...19902213410
active malaria transmission in south mizoram.anopheles dirus was incriminated as malaria vector with 1.12% sporozoite rate in tlabong (demagiri) subdivision, mizoram. high parity rate of a. dirus (33.69%) and a. minimus (52.87%) further confirmed their vectorial status. both the vectors were found highly susceptible to 4% ddt.19902209927
seasonal fluctuation of plasmodium falciparum gametocytaemia.two numerically minor components of plasmodium falciparum prevalence--gametocytaemia and trophozoite densities greater than 99/500 white blood cells--displayed an annual cycle that reflected the seasonal abundance of infective anopheles dirus at a hyperendemic focus in thailand, even though the gross monthly prevalence for combined ages remained stable. gametocyte prevalence rose more than 300% within 30 d after the capture of the dry season's first infective mosquito, remained at about 8% until ...19902189241
highly efficient dry season transmission of malaria in thailand.man-biting collections were made for 7 consecutive nights per month for 24 months at 2 sites in a thai village regularly treated with ddt and fenitrothion yet hyperendemic for plasmodium falciparum and p. vivax. only anopheles dirus was incriminated as a vector: 1.6% were infective and 2.4% were infected (median numbers of oocysts = 3.5). transmission occurred within the village, which was located in groves of rubber and fruit trees, during the dry months of november to may only, when rates of p ...19902189240
[analysis of amino acids in hemolymph and acid hydrolysates of midguts of anopheles dirus infected with plasmodium cynomolgi].quantitative determinations of free amino acids in hemolymph and acid hydrolysates of midguts of female anopheles dirus infected with plasmodium cynomolgi bastianellii were carried out and the results were compared with those of noninfected mosquitoes. on day 10 after infected blood meal, the contents of methionine, isoleucine, leucine, ornithine, lysine in the hemolymph of infected mosquitoes markedly decreased as compared with those in the controls. however, the quantitative analysis of the am ...19902099259
[induction of retarded exoerythrocytic schizonts by chloroguanide resulting in delayed parasitaemia of plasmodium inui in macaca mulatta].three rhesus monkeys inoculated with a large number of sporozoites of plasmodium inui dissected from the salivary glands of infected mosquitoes, anopheles dirus. two of the monkeys (inoculated with 8.06 x 10(5) and 1.3 x 10(7) sporozoites, respectively) were treated with chloroguanide base 6 mg/(kg.d) x 4 d starting from 24 h after the inoculations and the other one (inoculated with 5.93 x 10(6) sporozoites) was not treated with chloroguanide as control. the primary parasitaemia attacks occurred ...19902088005
plasmodium vivax: gametocyte infectivity of naturally infected thai adults.up to 200 laboratory reared anopheles dirus mosquitoes were fed on each of 496 symptomatic thai men who had patent, naturally acquired plasmodium vivax gametocytaemia. mean gametocyte densities were 455/mm3 (range: 0-3281), geometric mean oocyst number was 9 (0-142), mean frequency of infection was 43% (0-100%), and mean sporozoite number in salivary glands was 9525 (0-285,000). there was little relation between gametocyte density and either oocyst number or frequency of mosquito infection. ther ...19912038501
the number of sporozoites produced by individual malaria oocysts.mature oocysts of plasmodium falciparum and p. vivax from western thailand were separated from the midguts of anopheles dirus by collagenase digestion, and the number of sporozoites contained in each was counted. for 26 p. vivax oocysts, the mean count was 3, 688 (range 1, 954-5, 577) and for 14 p. falciparum, the mean count was 3, 385 (range 1, 359-4, 554); a single p. cynomolgi oocyst contained 7, 521. counts were not significantly correlated with oocyst density, oocyst age, or identity of the ...19911951866
comparative susceptibility of southeast asian anopheles mosquitoes to the simian malaria parasite plasmodium cynomolgi.seven anopheles species/isolates were compared with anopheles dirus (control) for susceptibility to plasmodium cynomolgi b strain. the mean numbers of oocysts in paired replicates of an. dirus and an. takasagoensis were not significantly different. the remaining test species had significantly fewer mean numbers of oocysts than anopheles dirus (p less than 0.01). anopheles dirus had the highest percentage of mosquitoes infected with p. cynomolgi sporozoites (82%). of the test groups, anopheles di ...19911791460
evaluation of monoclonal antibodies against plasmodium vivax sporozoites for elisa development.nine monoclonal antibodies (mabs) developed against plasmodium vivax (grassi & feletti) salivary gland sporozoites were evaluated for use in an enzyme-linked immunosorbent assay (elisa), using sporozoites developed in anopheles dirus peyton & harrison an. gambiae giles and an.maculatus theobald. four of the antibodies were unsuitable due to the low sensitivity of the resulting assays or the requirement for high concentrations of capture antibody. an additional two mabs were rejected because they ...19911768896
population genetic evidence for species a, b, c and d of the anopheles dirus complex in thailand and enzyme electromorphs for their identification.mixtures of chromosomal forms a, b, c and d in natural populations of anopheles dirus peyton & harrison sensu lato in thailand show significant positive values of wright's fixation index for six enzyme-electromorph loci. the mean value of fis over all loci was found to be +0.28 (sd 0.02), with a range of +0.57 (odh) to +0.10 (idh-2). partitioning electromorph data for the chromosomal forms reduces the mean fis to 0.03 (sd 0.01), which suggests that positive assortative mating is a characteristic ...19921600224
development of a polymorphic strain of plasmodium vivax in monkeys.a strain of plasmodium vivax from thailand with a polymorphic repeat unit of the circumsporozoite protein was established in saimiri sciureus boliviensis and 3 species of aotus monkeys. all 11 attempts to transmit infection via sporozoite inoculation, 4 times to splenectomized s. sciureus boliviensis, 2 times to splenectomized aotus nancymai, and 5 times to intact saimiri monkeys, were successful. anopheles freeborni, anopheles stephensi, anopheles dirus, and anopheles gambiae mosquitoes were in ...19921597793
anopheles dirus size and fecundity: relationship to larval density and protein accumulation.anopheles dirus were reared at two different larval densities (100/pan and 400/pan) to produce two different size classes of adults. both the wing length and fecundity of females from the two densities were significantly different (p less than 0.001). adult size was related to larval density and protein accumulation during immature life. egg production was also related to adult size. consequently, larger field adults have the potential to live longer and produce more eggs. the smaller adult size ...19921523465
[angkor. the mystery of the dead city and anopheles dirus].the desertion of angkor, which during more than five centuries was the center of a glorious civilization, has long been a matter of mystery and conjecture. the discovery of the vectorial capacity of the jungle mosquito anopheles dirus, its epidemiological importance in the emergence and spread of multidrug resistance in plasmodium falciparum malaria, the wiping out of large populations after transfer or deportation of non-immune khmers into forest areas can now easily explain the desertion of an ...19921494307
relationship between female anopheles dirus (diptera: culicidae) body size and parity in a biting population.wing length of host-seeking anopheles dirus peyton & harrison was measured in tha-mai district, chanthaburi province, thailand. overall, wing length of nulliparous females (mean = 3.035 mm) was not significantly smaller than that of parous females (mean = 3.039 mm). wing length was correlated with rainfall and minimum air temperature; females tended to be smaller in the rainy season and larger in the cool and early dry seasons, often in association with a high parity rate. malaria transmission p ...19921460629
adulticidal effect of cyfluthrin against mosquitos of public health importance in malaysia.the efficacy of two formulations, wettable powder and emulsifiable concentrate, of cyfluthrin sprayed on plywood [10 mg (ai)/m2] was assessed against six species of mosquitos. the bioassay followed the who standard method, with some modification for the bioassay of insecticidal deposits on wall surfaces. the results indicated that these two formulations of cyfluthrin were effective against anopheles dirus and mansonia uniformis, moderately toxic to aedes aegypti and ae. albopictus in decreasing ...19921355928
malaria transmission during post-spray period of pirimiphos-methyl in arunachal pradesh.observations made for a period of four years from 1985 to 1988 on post-spray impact of pirimiphos-methyl (25 per cent wp) on malaria vectors in tirap district of arunachal pradesh showed that a low density (0.0 to 0.02 pmh) of anopheles dirus was maintained in the areas sprayed with the insecticide at the dosages of 1 and 2 g/m2 from 1981 to 1984. the post-spray data (1985 to 1988) showed a reduction of 62.5 to 62.8 per cent in spr, 55.6 to 64.7 per cent in srf and 72.3 to 75.5 per cent decline ...19921344956
[distinguishing anopheles dirus of hainan province by gas chromatography of cuticular hydrocarbons].in order to identify the members of anopheles dirus complex of hainan province an analysis of relative content of cuticular saturated n-paraffins of anopheles dirus collected from different localities and in different months in hainan province was performed by using gas chromatography. the difference among the cuticular hydrocarbons n-paraffins components was statistically insignificant. the values of stepwise cluster analysis (q model) were very close for all the collected populations. these da ...19921303337
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