Publications
Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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addition of feruloyl esterase and xylanase produced on-site improves sugarcane bagasse hydrolysis. | accessory enzymes that assist biomass degradation could be used to improve the recovery of fermentable sugar for use in biorefineries. in this study, different fungal strains isolated from the amazon rainforest were evaluated in terms of their ability to produce feruloyl esterase (fae) and xylanase enzymes, and an assessment was made of the contributions of the enzymes in the hydrolysis of pretreated sugarcane bagasse. in the selection step, screening using plate assays was followed by shake fla ... | 2014 | 25151076 |
species-specific detection and identification of fusarium species complex, the causal agent of sugarcane pokkah boeng in china. | pokkah boeng disease caused by the fusarium species complex results in significant yield losses in sugarcane. thus, the rapid and accurate detection and identification of the pathogen is urgently required to manage and prevent the spreading of sugarcane pokkah boeng. | 2014 | 25141192 |
escherichia coli w shows fast, highly oxidative sucrose metabolism and low acetate formation. | sugarcane is the most efficient large-scale crop capable of supplying sufficient carbon substrate, in the form of sucrose, needed during fermentative feedstock production. however, sucrose metabolism in escherichia coli is not well understood because the two most common strains, e. coli k-12 and b, do not grow on sucrose. here, using a sucrose utilizing strain, e. coli w, we undertake an in-depth comparison of sucrose and glucose metabolism including growth kinetics, metabolite profiling, microa ... | 2014 | 25125039 |
synergistic effect of aspergillus niger and trichoderma reesei enzyme sets on the saccharification of wheat straw and sugarcane bagasse. | plant-degrading enzymes can be produced by fungi on abundantly available low-cost plant biomass. however, enzymes sets after growth on complex substrates need to be better understood, especially with emphasis on differences between fungal species and the influence of inhibitory compounds in plant substrates, such as monosaccharides. in this study, aspergillus niger and trichoderma reesei were evaluated for the production of enzyme sets after growth on two "second generation" substrates: wheat st ... | 2014 | 25116172 |
development of hemicellulolytic enzyme mixtures for plant biomass deconstruction on target biotechnological applications. | an essential step in the conversion of lignocellulosic biomass to ethanol and other biorefinery products is conversion of cell wall polysaccharides into fermentable sugars by enzymatic hydrolysis. the objective of the present study was to understand the mode of action of hemicellulolytic enzyme mixtures for pretreated sugarcane bagasse (psb) deconstruction and wheat arabinoxylan (wa) hydrolysis on target biotechnological applications. in this study, five hemicellulolytic enzymes-two endo-1,4-xyl ... | 2014 | 25077777 |
draft genome sequence of kluyveromyces marxianus strain dmb1, isolated from sugarcane bagasse hydrolysate. | we determined the genome sequence of a thermotolerant yeast, kluyveromyces marxianus strain dmb1, isolated from sugarcane bagasse hydrolysate, and the sequence provides further insights into the genomic differences between this strain and other reported k. marxianus strains. the genome described here is composed of 11,165,408 bases and has 4,943 protein-coding genes. | 2014 | 25059876 |
polyhydroxyalkanoate biosynthesis and simultaneous remotion of organic inhibitors from sugarcane bagasse hydrolysate by burkholderia sp. | burkholderia sp. f24, originally isolated from soil, was capable of growth on xylose and removed organic inhibitors present in a hemicellulosic hydrolysate and simultaneously produced poly-3-hydroxybutyrate (p3hb). using non-detoxified hydrolysate, burkholderia sp. f24 reached a cell dry weight (cdw) of 6.8 g l(-1), containing 48 % of p3hb and exhibited a volumetric productivity (pp3hb) of 0.10 g l(-1) h(-1). poly-3-hydroxybutyrate-co-3-hydroxyvalerate copolymers (p3hb-co-3hv) were produced usin ... | 2014 | 25059637 |
implications of stillage land disposal: a critical review on the impacts of fertigation. | stillage is the main wastewater from ethanol production, generated specifically in the step of distillation. regardless the feedstock, stillage contains high concentrations of organic matter, potassium and sulfates, as well as acidic and corrosive characteristics. currently almost the entire volume of stillage generated in brazilian distilleries is directed to the fertigation of sugarcane fields, due to its fertilizer character. however, the polluting potential of stillage characterizes its land ... | 2014 | 25058869 |
isolation, screening, and identification of cellulolytic bacteria from natural reserves in the subtropical region of china and optimization of cellulase production by paenibacillus terrae me27-1. | from different natural reserves in the subtropical region of china, a total of 245 aerobic bacterial strains were isolated on agar plates containing sugarcane bagasse pulp as the sole carbon source. of the 245 strains, 22 showed hydrolyzing zones on agar plates containing carboxymethyl cellulose after congo-red staining. molecular identification showed that the 22 strains belonged to 10 different genera, with the burkholderia genus exhibiting the highest strain diversity and accounting for 36.36 ... | 2014 | 25050355 |
perspectives on the production of polyhydroxyalkanoates in biorefineries associated with the production of sugar and ethanol. | polyhydroxyalkanoates (pha) are biodegradable and biocompatible bacterial thermoplastic polymers that can be obtained from renewable resources. the high impact of the carbon source in the final cost of this polymer has been one of the major limiting factors for pha production and agricultural residues, mainly lignocellulosic materials, have gained attention to overcome this problem. in brazil, production of 2nd generation ethanol from the glucose fraction, derived from sugarcane bagasse hydrolys ... | 2014 | 25043132 |
variation in chilling tolerance for photosynthesis and leaf extension growth among genotypes related to the c4 grass miscanthus ×giganteus. | the goal of this study was to identify cold-tolerant genotypes within two species of miscanthus related to the exceptionally chilling-tolerant c4 biomass crop accession: m. ×giganteus 'illinois' (mxg) as well as in other mxg genotypes. the ratio of leaf elongation at 10 °c/5 °c to that at 25 °c/25 °c was used to identify initially the 13 most promising miscanthus genotypes out of 51 studied. net leaf co2 uptake (a sat) and the maximum operating efficiency of photosystem ii (фpsii) were measured ... | 2014 | 25039073 |
evidence of environmental and vertical transmission of burkholderia symbionts in the oriental chinch bug, cavelerius saccharivorus (heteroptera: blissidae). | the vertical transmission of symbiotic microorganisms is omnipresent in insects, while the evolutionary process remains totally unclear. the oriental chinch bug, cavelerius saccharivorus (heteroptera: blissidae), is a serious sugarcane pest, in which symbiotic bacteria densely populate the lumen of the numerous tubule-like midgut crypts that the chinch bug develops. cloning and sequence analyses of the 16s rrna genes revealed that the crypts were dominated by a specific group of bacteria belongi ... | 2014 | 25038101 |
structural and physicochemical characteristics of starch from sugar cane and sweet sorghum stalks. | the starch present in sugar cane and sorghum juice has been considered a problem to the sugar industry. the objective of this work was to study the structural and physicochemical characteristics of the starch present in sugar cane and sweet sorghum. sugar cane and sweet sorghum starches presented small granules (maximum 5.9 and 7.9 μm), a-type diffraction pattern, high degree of relative crystallinity (44.4 and 42.0%), and low amylose content (17.5 and 16.4%), respectively. sugar cane starch pre ... | 2014 | 25037392 |
isolation of oleaginous yeast (rhodosporidium toruloides) mutants tolerant of sugarcane bagasse hydrolysate. | rhodosporidium toruloides is a lipid-producing yeast, the growth of which is severely suppressed when hydrolysates of lignocellulosic biomass are used as carbon source. this is probably due to the toxic substances, such as organic acids, furans, and phenolic compounds produced during the preparation of the hydrolysates. in order to solve this problem, r. toruloides cultures were subjected to atmospheric room-temperature plasma mutagenesis, resulting in the isolation of mutants showing tolerance ... | 2014 | 25036690 |
silicon reduces impact of plant nitrogen in promoting stalk borer (eldana saccharina) but not sugarcane thrips (fulmekiola serrata) infestations in sugarcane. | the stalk borer eldana saccharina walker (lepidoptera: pyralidae) is a major limiting factor in south african sugarcane production, while yield is also reduced by sugarcane thrips fulmekiola serrata kobus (thysanoptera: thripidae). borer management options include appropriate nitrogen (n) and enhanced silicon (si) nutrition; the effect of n on sugarcane thrips is unknown. we tested the effects of these nutrients, in combination with resistant (n33) and susceptible (n27) sugarcane cultivars, on e ... | 2014 | 24999349 |
screening of lactic acid bacteria for their potential as microbial cell factories for bioconversion of lignocellulosic feedstocks. | the use of fossil carbon sources for fuels and petrochemicals has serious impacts on our environment and is unable to meet the demand in the future. a promising and sustainable alternative is to substitute fossil carbon sources with microbial cell factories converting lignocellulosic biomass into desirable value added products. however, such bioprocesses require availability of suitable and efficient microbial biocatalysts, capable of utilizing c5 sugars and tolerant to inhibitory compounds gene ... | 2014 | 24997803 |
validation of novel reference genes for reverse transcription quantitative real-time pcr in drought-stressed sugarcane. | one of the most challenging aspects of rt-qpcr data analysis is the identification of reliable reference genes. ideally, they should be neither induced nor repressed under different experimental conditions. to date, few reference genes have been adequately studied for sugarcane (saccharum spp.) using statistical approaches. in this work, six candidate genes ( αtub, gapdh, h1, samdc, ubq, and 25s rrna) were tested for gene expression normalization of sugarcane root tissues from drought-tolerant a ... | 2014 | 24987730 |
building the sugarcane genome for biotechnology and identifying evolutionary trends. | sugarcane is the source of sugar in all tropical and subtropical countries and is becoming increasingly important for bio-based fuels. however, its large (10 gb), polyploid, complex genome has hindered genome based breeding efforts. here we release the largest and most diverse set of sugarcane genome sequences to date, as part of an on-going initiative to provide a sugarcane genomic information resource, with the ultimate goal of producing a gold standard genome. | 2014 | 24984568 |
effect of chemical factors on integrated fungal fermentation of sugarcane bagasse for ethanol production by a white-rot fungus, phlebia sp. mg-60. | bioethanol production through integrated fungal fermentation (iff), involving a unified process for biological delignification with consolidated biological processing by the white-rot fungus phlebia sp. mg-60, was applied to sugarcane bagasse. initial moisture content of the bagasse was found to affect biological delignification by mg-60, and 75% moisture content was suitable for selective lignin degradation and subsequent ethanol production. additives, such as basal media, organic compounds, or ... | 2014 | 24968109 |
association between aflatoxin b1 occupational airway exposure and risk of hepatocellular carcinoma: a case-control study. | the aim of this study was to determine the airway exposure of sugar and papermaking factory workers to aflatoxin b1 (afb1) and to explore the potential association between afb1 airway exposure and the risk of hepatocellular carcinoma (hcc) in a case-control study. dust samples were collected from the sugarcane bagasse warehouse, and presser and paper production workshops. blood samples were collected from 181 workshop employees and 203 controls who worked outside the workshop. afb1 albumin adduc ... | 2014 | 24961349 |
maize elongin c interacts with the viral genome-linked protein, vpg, of sugarcane mosaic virus and facilitates virus infection. | the viral genome-linked protein, vpg, of potyviruses is involved in viral genome replication and translation. to determine host proteins that interact with sugarcane mosaic virus (scmv) vpg, a yeast two-hybrid screen was used and a maize (zea mays) elongin c (zmelc) protein was identified. zmelc transcript was observed in all maize organs, but most highly in leaves and pistil extracts, and zmelc was present in the cytoplasm and nucleus of maize cells in the presence or absence of scmv. zmelc exp ... | 2014 | 24954157 |
molecular evolutionary history of sugarcane yellow leaf virus based on sequence analysis of rna-dependent rna polymerase and putative aphid transmission factor-coding genes. | rna-dependent rna polymerase (rdrp) encoded by orf2 and putative aphid transmission factor (patf) encoded by orf5 of sugarcane yellow leaf virus (scylv) were detected in six sugarcane cultivars affected by yellow leaf using rt-pcr and real-time rt-pcr assays. expression of both genes varied among infected plants, but overall expression of rdrp was higher than expression of patf. cultivar h87-4094 from hawaii yielded the highest transcript levels of rdrp, whereas cultivar c1051-73 from cuba exhib ... | 2014 | 24952671 |
filamentous fungi and media for cellulase production in solid state cultures. | cellulase production was evaluated in two reference strains (t. reesei rut-c30 and t. reesei qm9414), two strains isolated from a sugarcane cultivation area (trichoderma sp. ipt778 and t. harzianum rifai ipt821) and one strain isolated in a program for biodiversity preservation in são paulo state (myceliophthora thermophila m77). solid state cultures were performed using sugarcane bagasse (c), wheat bran (w) and/or soybean bran (s). the highest fpa was 10.6 u/gdm for m77 in sc (10:90) at 80% moi ... | 2014 | 24948946 |
reduced peroxisomal citrate synthase activity increases substrate availability for polyhydroxyalkanoate biosynthesis in plant peroxisomes. | polyhydroxyalkanoates (phas) are bacterial carbon storage polymers used as renewable, biodegradable plastics. pha production in plants may be a way to reduce industrial pha production costs. we recently demonstrated a promising level of peroxisomal pha production in the high biomass crop species sugarcane. however, further production strategies are needed to boost pha accumulation closer to commercial targets. through exogenous fatty acid feeding of arabidopsis thaliana plants that contain perox ... | 2014 | 24944109 |
prospecting sugarcane resistance to sugarcane yellow leaf virus by genome-wide association. | using gwas approaches, we detected independent resistant markers in sugarcane towards a vectored virus disease. based on comparative genomics, several candidate genes potentially involved in virus/aphid/plant interactions were pinpointed. yellow leaf of sugarcane is an emerging viral disease whose causal agent is a polerovirus, the sugarcane yellow leaf virus (scylv) transmitted by aphids. to identify quantitative trait loci controlling resistance to yellow leaf which are of direct relevance for ... | 2014 | 24916990 |
modeling evolution of resistance of sugarcane borer (lepidoptera: crambidae) to transgenic bt corn. | diatraea saccharalis (f.) (lepidoptera: crambidae) is a target pest of transgenic corn expressing bacillus thuringiensis (bt) protein, and the first evidence of resistance by d. saccharalis to cry1ab corn was detected in a field population in northeast louisiana in 2004. we used a model of population dynamics and genetics of d. saccharalis to 1) study the effect of interfield dispersal, the first date that larvae enter diapause for overwintering, toxin mortality, the proportion of non-bt corn in ... | 2014 | 24914780 |
biological neutralization and biosorption of dyes of alkaline textile industry wastewater. | the present work was aimed to secure biological neutralization and biosorption of dyes of an alkaline textile industrial effluent (atie) using an alkaliphilic bacterium, enterococcus faecalis strain r-16 isolated from gujarat coast. the isolate was capable and competent to bring down the ph of atie from 12.1 to 7.0 within 2 h in the presence of carbon and nitrogen sources. carboxylic group concentration (cgc), nmr and ft-ir analysis revealed production of carboxylic acid as a result of neutraliz ... | 2014 | 24910184 |
the impact of stress on the health of sugar cane cutters. | evaluate the impact of stress on sugar cane cutters and the prevalence of physical and psychological symptoms before and after harvest. | 2014 | 24897043 |
description of helicotylenchus persiaensis sp. n. (nematoda: hoplolaimidae) from iran . | in order to identify the species of helicotylenchus steiner, 1945 present in iran, 497 soil and root samples were collected from the rhizosphere of different plants and localities throughout the country during 2009-2010. a new and several known species of helicotylenchus were identified from the collected material. h. persiaensis sp. n. is characterized by its short tail (8-11 µm , c = 54.2-79.0, c' = 0.6-1.2), usually with smooth terminus or with 1-3 very coarse annules, rarely with minor ventr ... | 2014 | 24872247 |
new species and new records of tetranychidae (acarina, prostigmata) from thailand. | sampling efforts conducted in several provinces from thailand disclosed three new species of tetranychid mites. two of them belong to the genus tetranychus, namely tetranychus occultaspina sp. nov. and tetranychus truncatissimus sp. nov. and the third species belongs to the genus schizotetranychus, schizotetranychus krungthepensis sp. nov. they were collected on ipomoea aquatica, bambusa multiplex and saccharum officinarum, respectively. new records and new hosts are also mentioned. | 2014 | 24871006 |
defense response in non-genomic model species: methyl jasmonate exposure reveals the passion fruit leaves' ability to assemble a cocktail of functionally diversified kunitz-type trypsin inhibitors and recruit two of them against papain. | multiplicity of protease inhibitors induced by predators may increase the understanding of a plant's intelligent behavior toward environmental challenges. information about defense mechanisms of non-genomic model plant passion fruit (passiflora edulis sims) in response to predator attack is still limited. here, via biochemical approaches, we showed its flexibility to build-up a broad repertoire of potent kunitz-type trypsin inhibitors (ktis) in response to methyl jasmonate. seven inhibitors (20- ... | 2014 | 24849173 |
comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-pcr. | the increasingly used real time quantitative reverse transcription-pcr (qrt-pcr) method for gene expression analysis requires one or several reference gene(s) acting as normalization factor(s). in order to facilitate gene expression studies in sugarcane (saccharum officinarum), a non-model plant with limited genome information, the stability of 13 candidate reference genes was evaluated. the genorm, normfinder and deltact methods were used for selecting stably expressed internal controls across ... | 2014 | 24823940 |
characterization of small interfering rnas derived from sugarcane mosaic virus in infected maize plants by deep sequencing. | rna silencing is a conserved surveillance mechanism against viruses in plants. it is mediated by dicer-like (dcl) proteins producing small interfering rnas (sirnas), which guide specific argonaute (ago)-containing complexes to inactivate viral genomes and may promote the silencing of host mrnas. in this study, we obtained the profile of virus-derived sirnas (vsirnas) from sugarcane mosaic virus (scmv) in infected maize (zea mays l.) plants by deep sequencing. our data showed that vsirnas which d ... | 2014 | 24819114 |
utilization of agricultural residues of pineapple peels and sugarcane bagasse as cost-saving raw materials in scenedesmus acutus for lipid accumulation and biodiesel production. | the aim of this study is to optimize the lipid accumulation in microalgae by using two agricultural residues of pineapple peels and sugarcane bagasse as low-cost organic carbon sources. green microalgae scenedesmus acutus was isolated and selected for cultivation. effects of three initial sugar concentrations and the stage for adding sugar during cultivation on biomass and lipid production were investigated. the results clearly showed that two-stage cultivation is more suitable than one-stage. t ... | 2014 | 24817554 |
a high degree of african streak virus diversity within nigerian maize fields includes a new mastrevirus from axonopus compressus. | the a-strain of maize streak virus (msv-a; genus mastrevirus, family geminiviridae), the causal agent of maize streak disease, places a major constraint on maize production throughout sub-saharan africa. in west-african countries such as nigeria, where maize is not cultivated year-round, this msv strain is forced to overwinter in non-maize hosts. in order to both identify uncultivated grasses that might harbour msv-a during the winter season and further characterise the diversity of related maiz ... | 2014 | 24796552 |
digesta sampling sites and marker methods for estimation of ruminal outflow in bulls fed different proportions of corn silage or sugarcane. | the objective of this experiment was to assess ruminal outflow and apparent total-tract digestibility using digesta samples from 3 sites (reticulum, omasum, and abomasum) and 3 marker methods (single marker: indigestible ndf [indf; sample without separation]; double marker: indf + co-edta [filtered sample]; and triple marker: indf + ytterbium [yb] acetate + co-edta [filtered and centrifuged]) in bulls fed corn silage and sugarcane-based diets. eight crossbred (holstein × zebu) bulls (353 ± 37 kg ... | 2014 | 24778340 |
biogeographical variation and population genetic structure of sporisorium scitamineum in mainland china: insights from issr and sp-srap markers. | a total of 100 sporisorium scitamineum isolates were investigated by inter simple sequence repeat (issr) and single primer-sequence related amplified polymorphism (sp-srap) markers. these isolates were clearly assorted into three distinct clusters regardless of method used: either cluster analysis or by principal component analysis (pca) of the issr, sp-srap, or issr + sp-srap data set. the total gene diversity (h t) and gene diversity between subpopulations (h s) were estimated to be 0.34 to 0. ... | 2014 | 24772015 |
identification and characterisation of xylanolytic yeasts isolated from decaying wood and sugarcane bagasse in brazil. | in this study, yeasts associated with lignocellulosic materials in brazil, including decaying wood and sugarcane bagasse, were isolated, and their ability to produce xylanolytic enzymes was investigated. a total of 358 yeast isolates were obtained, with 198 strains isolated from decaying wood and 160 strains isolated from decaying sugarcane bagasse samples. seventy-five isolates possessed xylanase activity in solid medium and were identified as belonging to nine species: candida intermedia, c. t ... | 2014 | 24748334 |
multi-scale structural and chemical analysis of sugarcane bagasse in the process of sequential acid-base pretreatment and ethanol production by scheffersomyces shehatae and saccharomyces cerevisiae. | heavy usage of gasoline, burgeoning fuel prices, and environmental issues have paved the way for the exploration of cellulosic ethanol. cellulosic ethanol production technologies are emerging and require continued technological advancements. one of the most challenging issues is the pretreatment of lignocellulosic biomass for the desired sugars yields after enzymatic hydrolysis. we hypothesized that consecutive dilute sulfuric acid-dilute sodium hydroxide pretreatment would overcome the native r ... | 2014 | 24739736 |
selfing rate estimation in sugarcane under unfavorable natural conditions of crossing by using microsatellite markers. | the self-fertilization or selfing rate estimation using microsatellite markers and its impact on survival and selection rate were evaluated in families derived from polycrosses that involved parents that were widely used in sugarcane breeding in brazil. these factors were evaluated under unfavorable natural conditions of flowering and crossing. after the germination test, the viable progeny were taken to the field for survival rate evaluation (4, 6, and 10 months) and phenotypic selection at pla ... | 2014 | 24737476 |
paternity identification in sugarcane polycrosses by using microsatellite markers. | although polycrosses have been used to test the potential of cross-combination of a large number of sugarcane parents, the male parent of the half-sib progenies produced is unknown. the present study aimed to integrate the molecular marker technology to the sugarcane polycross approach by the application of microsatellite markers to identify the male parent of 41 elite clones derived from polycross families. ten microsatellite [single sequence repeats (ssrs)] primer pairs were used to identify t ... | 2014 | 24737475 |
managing the sugarcane borer, diatraea saccharalis, and corn earworm, helicoverpa zea, using bt corn and insecticide treatments. | the sugarcane borer, diatraea saccharalis (fabricius) (lepidoptera: crambidae) and the corn earworm, helicoverpa zea (boddie) (lepidoptera: noctuidae), are important pests of corn in brazil and have not been successfully managed, because of the difficulty of managing them with pesticides. the objective of this study was to evaluate the effect of bt corn mon810, transformed with a gene from bacillus thuringiensis berliner (bacillales: bacillaceae) insecticide seed treatment, and foliar insecticid ... | 2013 | 24735131 |
phosphate enhances levan production in the endophytic bacterium gluconacetobacter diazotrophicus pal5. | gluconacetobacter diazotrophicus is a gram-negative and endophytic nitrogen-fixing bacterium that has several beneficial effects in host plants; thus, utilization of this bacterium as a biofertilizer in agriculture may be possible. g. diazotrophicus synthesizes levan, a d-fructofuranosyl polymer with β-(2→6) linkages, as an exopolysaccharide and the synthesized levan improves the stress tolerance of the bacterium. in this study, we found that phosphate enhances levan production by g. diazotrophi ... | 2014 | 24717418 |
bioethanol production from fermentable sugar juice. | bioethanol production from renewable sources to be used in transportation is now an increasing demand worldwide due to continuous depletion of fossil fuels, economic and political crises, and growing concern on environmental safety. mainly, three types of raw materials, that is, sugar juice, starchy crops, and lignocellulosic materials, are being used for this purpose. this paper will investigate ethanol production from free sugar containing juices obtained from some energy crops such as sugarca ... | 2014 | 24715820 |
the impact of dietary sugarcane addition to finishing diets on performance, apparent digestibility, and fatty acid composition of holstein × zebu bulls. | the objective of this study was to evaluate the influence of corn silage (cs) or sugarcane (sc) additions to finishing diets on voluntary intake, apparent digestibility, animal performance, and lm fatty acid (fa) composition in crossbred holstein × zebu bulls. forty-two bulls (initial bw 328 ± 39.2 kg and an initial age of 23.5 mo) were used in this experiment. a completely randomized design was used to examine roughage source (cs vs. sc) and ratio of concentrate (co) on nutrient utilization and ... | 2014 | 24713164 |
draft genome sequences of xanthomonas sacchari and two banana-associated xanthomonads reveal insights into the xanthomonas group 1 clade. | we present draft genome sequences for three strains of xanthomonas species, each of which was associated with banana plants (musa species) but is not closely related to the previously sequenced banana-pathogen xanthomonas campestris pathovar musacearum. strain ncppb4393 had been deposited as xanthomonas campestris pathovar musacearum but in fact falls within the species xanthomonas sacchari. strain ncppb1132 is more distantly related to xanthomonas sacchari whilst strain ncppb 1131 grouped in a ... | 2011 | 24710305 |
evaluation of the tolerance of acetic acid and 2-furaldehyde on the growth of pichia stipitis and its respiratory deficient. | the use of lignocellulosic residues for ethanol production is limited by toxic compounds in fermenting yeasts present in diluted acid hydrolysates like acetic acid and 2-furaldehyde. the respiratory deficient phenotype gives the cell the ability to resist several toxic compounds. so the aim of this work was to evaluate the tolerance to toxic compounds present in lignocellulosic hydrolysates like acetic acid and 2-furaldehyde in pichia stipitis and its respiratory deficient strains. the respirato ... | 2014 | 24700134 |
impact of nano-caco3 -ldpe packaging on quality of fresh-cut sugarcane. | in order to evaluate the effects of nano-caco3 -based low density polyethylene (nano-caco3 -ldpe) packaging on the quality of fresh-cut sugarcane, concentrations of o2 and co2 within the packages, overall visual quality (ovq), total bacterial count (tbc), yeast and mould count (ymc), reducing sugar content and total phenolic content, respiration, ethylene production, and the activities of phenylalanine ammonia-lyase (pal), polyphenol oxidase (ppo), peroxidase (pod), acid invertase (ai) and neutr ... | 2014 | 24700113 |
pseudozyma brasiliensis sp. nov., a xylanolytic, ustilaginomycetous yeast species isolated from an insect pest of sugarcane roots. | a novel ustilaginomycetous yeast isolated from the intestinal tract of an insect pest of sugarcane roots in ribeirão preto, são paulo state, brazil, represents a novel species of the genus pseudozyma based on molecular analyses of the d1/d2 rdna large subunit and the internal transcribed spacer (its1+its2) regions. the name pseudozyma brasiliensis sp. nov. is proposed for this species, with ghg001(t) ( = cbs 13268(t) = ufmg-cm-y307(t)) as the type strain. p. brasiliensis sp. nov. is a sister spe ... | 2014 | 24682702 |
ethanol fermentation from molasses at high temperature by thermotolerant yeast kluyveromyces sp. iipe453 and energy assessment for recovery. | high temperature ethanol fermentation from sugarcane molasses b using thermophilic crabtree-positive yeast kluyveromyces sp. iipe453 was carried out in batch bioreactor system. strain was found to have a maximum specific ethanol productivity of 0.688 g/g/h with 92 % theoretical ethanol yield. aeration and initial sugar concentration were tuning parameters to regulate metabolic pathways of the strain for either cell mass or higher ethanol production during growth with an optimum sugar to cell rat ... | 2014 | 24682264 |
modeling the microbial growth and temperature profile in a fixed-bed bioreactor. | aiming to scale up and apply control and optimization strategies, currently is required the development of accurate plant models to forecast the process nonlinear dynamics. in this work, a mathematical model to predict the growth of the kluyveromyces marxianus and temperature profile in a fixed-bed bioreactor for solid-state fermentation using sugarcane bagasse as substrate was built up. a parameter estimation technique was performed to fit the mathematical model to the experimental data. the es ... | 2014 | 24658796 |
one-pot green synthesis of carbon dots by using saccharum officinarum juice for fluorescent imaging of bacteria (escherichia coli) and yeast (saccharomyces cerevisiae) cells. | we are reporting highly economical plant-based hydrothermal method for one-pot green synthesis of water-dispersible fluorescent carbon dots (cds) by using saccharum officinarum juice as precursor. the synthesized cds were characterized by uv-visible, fluorescence, fourier transform infrared (ft-ir), dynamic light scattering (dls), high-resolution transmission electron microscopic (hr-tem), and laser scanning confocal microscopic techniques. the cds are well dispersed in water with an average siz ... | 2014 | 24656348 |
induced over-expression of atdreb2a ca improves drought tolerance in sugarcane. | drought is one of the most challenging agricultural issues limiting sustainable sugarcane production and, in some cases, yield losses caused by drought are nearly 50%. dreb proteins play vital regulatory roles in abiotic stress responses in plants. the transcription factor dreb2a interacts with a cis-acting dre sequence to activate the expression of downstream genes that are involved in drought-, salt- and heat-stress response in arabidopsis thaliana. in the present study, we evaluated the effec ... | 2014 | 24656336 |
selecting low-cost carbon sources for carotenoid and lipid production by the pink yeast rhodosporidium toruloides ncyc 921 using flow cytometry. | the present work studied low-cost carbon sources for carotenoid and lipid production using the yeast rhodosporidum toruloides ncyc 921. carob pulp syrup and sugarcane molasses at different concentrations were used as low-cost carbon sources in r. toruloides batch cultivations. carob pulp syrup containing a total sugar concentration of 75 g l(-1) induced the highest total fatty acid productivity (1.90 g l(-1)h(-1)) and the highest carotenoid productivity (9.79 μg l(-1)h(-1)). flow cytometric anal ... | 2014 | 24650616 |
extraction optimization by response surface methodology: purification and characterization of phytosterol from sugarcane (saccharum officinarum l.) rind. | a green, simple, and effective method for the extraction of sugarcane lipids from sugarcane rind was investigated by response surface methodology. the optimum conditions of technological progress obtained through response surface methodology were as follows: liquid-to-solid ratio 7.94: 1 ml/g, extraction temperature 50°c and extraction time 5.98 h. the practical sugarcane lipids extraction yield was 6.55 ± 0.28%, which was in good consistence with the predicted extraction yield of 6.47%. the res ... | 2014 | 24648272 |
organic loading rate impact on biohydrogen production and microbial communities at anaerobic fluidized thermophilic bed reactors treating sugarcane stillage. | this study aimed to evaluate the effect of high organic loading rates (olr) (60.0-480.00 kg cod m(-3)d(-1)) on biohydrogen production at 55°c, from sugarcane stillage for 15,000 and 20,000 mg codl(-1), in two anaerobic fluidized bed reactors (afbr1 and afbr2). it was obtained, for h2 yield and content, a decreasing trend by increasing the olr. the maximum h2 yield was observed in afbr1 (2.23 mmol g cod added(-1)). the volumetric h2 production was proportionally related to the applied hydraulic r ... | 2014 | 24632626 |
genetic variability among the chloroplast genomes of sugarcane (saccharum spp) and its wild progenitor species saccharum spontaneum l. | a striking characteristic of modern sugarcane is that all sugarcane cultivars (saccharum spp) share a common cytoplasm from s. officinarum. to explore the potential value of s. spontaneum cytoplasm, new saccharum hybrids with an s. spontaneum cytoplasm were developed at the united states department of agriculture-agricultural research service, sugarcane research laboratory, through a combination of conventional and molecular breeding approaches. in this study, we analyzed the genetic variability ... | 2014 | 24615073 |
the sugarcane defense protein sugarwin2 causes cell death in colletotrichum falcatum but not in non-pathogenic fungi. | plants respond to pathogens and insect attacks by inducing and accumulating a large set of defense-related proteins. two homologues of a barley wound-inducible protein (barwin) have been characterized in sugarcane, sugarwin1 and sugarwin2 (sugarcane wound-inducible proteins). induction of sugarwins occurs in response to diatraea saccharalis damage but not to pathogen infection. in addition, the protein itself does not show any effect on insect development; instead, it has antimicrobial activitie ... | 2014 | 24608349 |
process optimization and production kinetics for cellulase production by trichoderma viride vkf3. | microbial cellulases are the enzymes widely studied due to their enormous applications in biochemical industry. among 12 fungal isolates isolated from mangrove plant debris and soil sample collected from valanthakad mangroves, kerala, india, 3 of them were found to exhibit cellulolytic activity. among them, the most potent isolate which exhibited maximum cellulolytic activity was identified as trichoderma viride vkf3 [gene bank accession number- jx683684.1] based on colony morphology, microscopi ... | 2014 | 24600546 |
construction of individual, fused, and co-expressed proteins of endoglucanase and β-glucosidase for hydrolyzing sugarcane bagasse. | at least a combination of endoglucanase (eglii) and β-glucosidase (bglz) is required for hydrolyzing crystalline cellulose. to understand the catalytic efficiency of combination enzymes for converting biomass to sugars, eglii and bglz were constructed in the form of individual, fused as well as co-expression proteins, and their activities for hydrolyzing sugarcane bagasse were evaluated. the genes, eglii isolated from bacillus amyloliquefaciens psm3.1 earlier and bglz from b. amyloliquefaciens a ... | 2016 | 24598011 |
secretomic survey of trichoderma harzianum grown on plant biomass substrates. | the present work aims at characterizing t. harzianum secretome when the fungus is grown in synthetic medium supplemented with one of the four substrates: glucose, cellulose, xylan, and sugarcane bagasse (sb). the characterization was done by enzymatic assays and proteomic analysis using 2-de/maldi-tof and gel-free shotgun lc-ms/ms. the results showed that sb induced the highest cellulolytic and xylanolytic activities when compared with the other substrates, while remarkable differences in terms ... | 2014 | 24593137 |
mating populations of fusarium section liseola from rice, sugarcane and maize. | mating studies were conducted on 78 isolates of fusarium species section liseola from rice, sugarcane and maize. from the crosses with tester strains of gibberella fujikuroi species complex, 64.1% (50 out of 78 isolates) were cross-fertile with tester strains of mating populations a to e. the results of the mating studies showed that of the 50 isolates, 19 belonged to mating population a (gibberella moniliformis), 18 to mating population b (gibberella sacchari), 4 to mating population e (gibbere ... | 2011 | 24575220 |
a comprehensive genetic map of sugarcane that provides enhanced map coverage and integrates high-throughput diversity array technology (dart) markers. | sugarcane genetic mapping has lagged behind other crops due to its complex autopolyploid genome structure. modern sugarcane cultivars have from 110-120 chromosomes and are in general interspecific hybrids between two species with different basic chromosome numbers: saccharum officinarum (2n = 80) with a basic chromosome number of 10 and s. spontaneum (2n = 40-128) with a basic chromosome number of 8. the first maps that were constructed utilised the single dose (sd) markers generated using rflp, ... | 2014 | 24564784 |
transcriptome profile of trichoderma harzianum ioc-3844 induced by sugarcane bagasse. | profiling the transcriptome that underlies biomass degradation by the fungus trichoderma harzianum allows the identification of gene sequences with potential application in enzymatic hydrolysis processing. in the present study, the transcriptome of t. harzianum ioc-3844 was analyzed using rna-seq technology. the sequencing generated 14.7 gbp for downstream analyses. de novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities. ... | 2014 | 24558413 |
effect of feeding strategies on lipid production by lipomyces starkeyi. | the aim of this study was to produce microbial oil from lipomyces starkeyi dsm 70296 grown in hemicellulose hydrolysate (h-h). glucose and xylose were used for batch, fed-batch, repeated fed-batch, and continuous cultures, and h-h was tested at continuous culture. the highest cell and lipid concentrations of 85.4 and 41.8g/l, respectively, were obtained using repeated fed-batch strategy. continuous culture with dilution rate of 0.03h(-1) presented the highest overall cell (0.443g/g) and lipid yi ... | 2014 | 24556374 |
genetic diversity in sugarcane varieties in brazil based on the ward-modified location model clustering strategy. | we evaluated the genetic diversity of 77 clones of sugarcane used in crosses made by the brazilian interuniversity network for the development of the sugar/energy sector (ridesa) breeding program. characterization of the genotypes was carried out at the ratoon stage, based on eight morphological traits and seven agronomic traits. diversity analysis was carried out beginning with the ward-modified location model. the ideal number of groups was three. groups 1, 2, and 3 were composed of 37, 21, an ... | 2014 | 24535897 |
pyraclostrobin wash-off from sugarcane leaves and aerobic dissipation in agricultural soil. | to mitigate damage from the fungal sugarcane pathogen brown rust (puccinia melanocephala), a section 18 emergency use label was put in place by the united states environmental protection agency (u.s. epa) for the application of pyraclostrobin (trade name headline sc, produced by basf, research triangle park, nc) on sugarcane in 2008. to assess the dynamics of this fungicide in louisiana soil, samples (n = 24) from a non-treated field were spiked with pyraclostrobin (3.1 μg g(-1)) and analyzed in ... | 2014 | 24533668 |
control of diatraea saccharalis by the endophytic pantoea agglomerans 33.1 expressing cry1ac7. | despite the fact that bacillus thuringiensis (bt) is found in more than 90 % of the products used against insects, it has some difficulty reaching the internal regions where the larvae feed. to solve this problem, many genetically modified microorganisms that colonize the same pests have been developed. thus, the endophytic bacterium pantoea agglomerans (33.1), which has been recently described as a promising sugarcane growth promoter, was genetically modified with the pjtt vector (which carries ... | 2014 | 24531524 |
using multibiomarker approach as a tool to improve the management plan for a private reserve of natural heritage (rppn). | this study aimed to monitor an aquatic ecosystem during two different periods (dry and rainy season) on a protected area located inside a sugarcane farm, using the fish astyanax sp. as bioindicator. an integrated approach was adopted by combining the responses of well-known biomarkers: acetylcholinesterase, lipid peroxidation (lpo), catalase (cat), glutathione s-transferase (gst), micronucleus test, and liver histopathology. the activity of enzymes cat and gst was increased after the rainy seaso ... | 2014 | 24531322 |
structural and functional characterization of the chaperone hsp70 from sugarcane. insights into conformational changes during cycling from cross-linking/mass spectrometry assays. | hsp70 cycles from an atp-bound state, in which the affinity for unfolded polypeptides is low, to an adp-bound state, in which the affinity for unfolded polypeptides is high, to assist with cell proteostasis. such cycling also depends on co-chaperones because these proteins control both the hsp70 atpase activity and the delivery of unfolded polypeptide chains. although it is very important, structural information on the entire protein is still scarce. this work describes the first cloning of a cd ... | 2014 | 24530624 |
assessment of in vitro cyto/genotoxicity of sequentially treated electroplating effluent on the human hepatocarcinoma huh-7 cell line. | the present study compares in vitro toxicity of electroplating effluent after the batch treatment process with that obtained after the sequential treatment process. activated charcoal prepared from sugarcane bagasse through chemical carbonization, and tolerant indigenous bacteria, bacillus sp. strain ist105, were used individually and sequentially for the treatment of electroplating effluent. the sequential treatment involving activated charcoal followed by bacterial treatment removed 99% of cr( ... | 2014 | 24525376 |
de novo assembly and transcriptome analysis of contrasting sugarcane varieties. | sugarcane is an important crop and a major source of sugar and alcohol. in this study, we performed de novo assembly and transcriptome annotation for six sugarcane genotypes involved in bi-parental crosses. the de novo assembly of the sugarcane transcriptome was performed using short reads generated using the illumina rna-seq platform. we produced more than 400 million reads, which were assembled into 72,269 unigenes. based on a similarity search, the unigenes showed significant similarity to mo ... | 2014 | 24523899 |
breaking dogmas: the plant vascular pathogen xanthomonas albilineans is able to invade non-vascular tissues despite its reduced genome. | xanthomonas albilineans, the causal agent of sugarcane leaf scald, is missing the hrp type iii secretion system that is used by many gram-negative bacteria to colonize their host. until now, this pathogen was considered as strictly limited to the xylem of sugarcane. we used confocal laser scanning microscopy, immunocytochemistry and transmission electron microscopy (tem) to investigate the localization of x. albilineans in diseased sugarcane. sugarcane plants were inoculated with strains of the ... | 2014 | 24522883 |
solving ethanol production problems with genetically modified yeast strains. | the current world demand for bioethanol is increasing as a consequence of low fossil fuel availability and a growing number of ethanol/gasoline flex-fuel cars. in addition, countries in several parts of the world have agreed to reduce carbon dioxide emissions, and the use of ethanol as a fuel (which produces fewer pollutants than petroleum products) has been considered to be a good alternative to petroleum products. the ethanol that is produced in brazil from the first-generation process is opti ... | 2013 | 24516432 |
exploration of potential baker's yeast from sugarcane juice: optimization and evaluation. | the present study was carried out to explore baker's yeasts strains from sugarcane juice to assess its potential in laboratory scale production of breads. collected juice samples were processed for isolation and identification of yeast strains based on standard cultural, morphological and biochemical characteristics. among the six isolated strains, four (designated as s1, s2, s5 and s6) were identified as saccharomyces cerevisiae and the rests (designated s3 and s4) were as s. rouxii. when asses ... | 2013 | 24505984 |
fitness costs and stability of cry1ab resistance in sugarcane borer, diatraea saccharalis (f.). | the sugarcane borer, diatraea saccharalis (f.), is a major target species of transgenic corn expressing bacillus thuringiensis (bt) proteins in south america and the u.s. mid-south region. in this study, the fitness of seven insect genotypes of d. saccharalis were assayed on non-toxic diet, which included a cry1ab-susceptible strain (ss-2009), two cry1ab-resistant strains (rr-43a(bc), rr-l5b(bc)), and four f1 hybrids (f1-r43a(m)s(f), f1-r43a(f)s(m), f1-r5b(m)s(f), and f1-r5b(f)s(m)). the f1 hybr ... | 2014 | 24503242 |
genetic analysis of resistance to six virus diseases in a multiple virus-resistant maize inbred line. | novel and previously known resistance loci for six phylogenetically diverse viruses were tightly clustered on chromosomes 2, 3, 6 and 10 in the multiply virus-resistant maize inbred line, oh1vi. virus diseases in maize can cause severe yield reductions that threaten crop production and food supplies in some regions of the world. genetic resistance to different viruses has been characterized in maize populations in diverse environments using different screening techniques, and resistance loci hav ... | 2014 | 24500307 |
leaf microbodies (peroxisomes) and catalase localization in plants differing in their photosynthetic carbon pathways. | the tropical grasses sugarcane (saccharum officinarum) and pangolagrass (digitaria decumbens) contained fewer leaf microbodies than temperate orchardgrass (dactylis glomerata). leaf microbodies were seen in both the mesophyll and bundle sheath cells of tropical grasses. the fibrous elements in the microbodies of tropical grasses differed from those of the temperate grass. catalase was predominantly localized in the microbodies of leaf cells (3,3'-diaminobenzidine method). the site of greatest ca ... | 1971 | 24493219 |
[differences in the light-activation of nadp-dependent glyceraldehyde-3-phosphate dehydrogenase and of ribulose-5-phosphate kinase between plants containing the calvin and those containing the c4-dicarboxylic acid pathway of photosynthetic carbon reduction]. | 1. preceding experiments had shown that irradiance of intact leaves or of isolated chloroplasts causes a reversible increase in the activity of nadp-gpd (ziegler and ziegler, 1965) as well as of ribulose-5-phosphate kinase (latzko and gibbs, 1969). examination of several species which carry out the calvin type of photosynthetic co2 fixation (vicia faba, spinacia oleracea, nicotiana tabacum, avena sativa) now revealed that the dark level of nadp-gpd activity ranges between 300-400 μmol nadph/mg c ... | 1971 | 24493083 |
expression analysis of sugarcane aquaporin genes under water deficit. | the present work is a pioneer study specifically addressing the aquaporin transcripts in sugarcane transcriptomes. representatives of the four aquaporin subfamilies (pip, tip, sip, and nip), already described for higher plants, were identified. forty-two distinct aquaporin isoforms were expressed in four ht-supersage libraries from sugarcane roots of drought-tolerant and -sensitive genotypes, respectively. at least 10 different potential aquaporin isoform targets and their respective unitags wer ... | 2013 | 24490055 |
physiological and photosynthetic characteristics of indica hang2 expressing the sugarcane pepc gene. | phosphoenolpyruvate carboxylase (pepc) is known to play a key role in the initial fixation of co2 in c4 photosynthesis. the pepc gene from sugarcane (a c4 plant) was introduced into indica rice (hang2), a process mediated by agrobacterium tumefaciens. integration patterns and copy numbers of the gene was confirmed by dna blot analysis. rt-pcr and western blotting results showed that the pepc gene was expressed at both the mrna and protein levels in the transgenic lines. real-time pcr results ind ... | 2014 | 24469712 |
valorization of sugarcane bagasse ash: producing glass-ceramic materials. | some aluminosilicates, for example mullite and wollastonite, are very important in the ceramic and construction industries. the most significant glass-ceramic for building applications has wollastonite as the main crystal phase. in this work we report on the use of sugarcane bagasse ash (scba) to produce glass-ceramics with silicates as the major crystalline phases. the glasses (frits) were prepared by mixing ash, limestone (calcium and magnesium carbonates) and potassium carbonate as the fluxin ... | 2014 | 24463731 |
isolation and identification of hexazinone-degrading bacterium from sugarcane-cultivated soil in kenya. | the s-triazine herbicide hexazinone [3-cyclohexyl-6-dimethylamino-1-methyl-1,3,5-triazine-2,4(1h,3h)-dione], is widely used in agriculture for weed control. laboratory biodegradation experiments for hexazinone in liquid cultures were carried out using sugarcane-cultivated soils in kenya. liquid culture experiments with hexazinone as the only carbon source led to the isolation of a bacterial strain capable of its degradation. through morphological, biochemical and molecular characterization by 16 ... | 2014 | 24458247 |
diversity of culturable yeasts in phylloplane of sugarcane in thailand and their capability to produce indole-3-acetic acid. | yeasts were isolated by the enrichment technique from the phylloplane of 94 samples of sugarcane leaf collected from seven provinces in thailand. all sugarcane leaf samples contained yeasts and 158 yeast strains were obtained. on the basis of the d1/d2 domain of the large subunit rrna gene sequence analysis, 144 strains were identified to 24 known species in 14 genera belonging to the ascomycota viz. candida akabanensis, candida dendronema, candida mesorugosa, candida michaelii, candida nivarien ... | 2014 | 24442819 |
localization of the c4 and c 3 pathways of photosynthesis in the leaves of pennisetum purpureum and other c4 species. insignificance of phenol oxidase. | mesophyll protoplasts and bundle-sheath cells of pennisetum purpureum schum., a c4 plant with low phenol-oxidase activity, were enzymatically separated according to methods recently developed with sugarcane (saccharum officinarum l.), maize (zea mays l.), and sorghum (sorghum bicolor l.). the phosphoenolpyruvate carboxylase and nadp-malic dehydrogenase of the c4 pathway were found to be localized in the mesophyll protoplasts while ribulose-1,5-diphosphate (rudp) carboxylase, phosphoribulokinase ... | 1974 | 24442563 |
evaluating the composition and processing potential of novel sources of brazilian biomass for sustainable biorenewables production. | the search for promising and renewable sources of carbohydrates for the production of biofuels and other biorenewables has been stimulated by an increase in global energy demand in the face of growing concern over greenhouse gas emissions and fuel security. in particular, interest has focused on non-food lignocellulosic biomass as a potential source of abundant and sustainable feedstock for biorefineries. here we investigate the potential of three brazilian grasses (panicum maximum, pennisetum p ... | 2014 | 24438499 |
quantitative genetics of sugarcane : ii. correlation analysis of continuous characters in relation to hybrid sugarcane breeding. | 1. a study has been made of the genetic, clonal and environmental correlations existing among commercially important characters in a sugarcane breeding population of interspecific hybrid origin. 2. in general, there is a close correspondence between genetic correlation coefficients measuring the extent of association at the level of family means, and clonal correlations measuring genotypic relationships at the level of clone means. greater weight has therefore been given to the numerical estimat ... | 1969 | 24435213 |
in vitro selection and characterization of polyethylene glycol (peg) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (saccharum officinarum l.). | a system for in vitro selection of drought tolerant callus lines in sugarcane was developed. high molecular weight peg was used as selective agent. selected callus line grew better than non-selected callus when grown on different concentrations of peg. the activity of antioxidant enzymes like cat, pox, apx and sod were high in selected callus than in non-selected callus. osmolytes like proline and ascorbic acid were at higher levels in selected callus than in non-selected callus, however at high ... | 2013 | 24431494 |
suitability of some local agro-industrial wastes as carrier materials forrhizobium sp. infectingsesbania bispinosa. | two indigenous rhizobial strains (sb-1 and jjs-1) infectingsesbania bispinosa survived at room temperature (32±2°c) up to 90 days in filtermud (the best system), peat, charcoal, sugarcane bagasse and sawdust (the poorest system). viable counts remained more than 10(8)/g up to 75 days in filtermud, peat and charcoal. | 1992 | 24425491 |
bacterial flora in the rhizosphere of sugarcane plant, saccharum officinarum. | streptomycin-resistant bacteria were recovered from the root-free soils under sugarcane plants. nitrosomonas species and azotobacter chrococcum were also present in greater numbers in rhizosphere soils as compared to root-free soils up to 30 cm deep. | 1991 | 24425034 |
production of fructooligosaccharides from sucrose by a transfructosylase from aspergillus niger. | a strain of aspergillus niger isolated from sugarcane fields, produced an extracellular transfructosylase in the culture medium. sucrose and raffinose induced the production to the enzyme, which was purified by 138-fold. the optimum ph for activity and stability were 5.5 and 6.5, respectively. its optimum temperature was 55°c. the enzyme hydrolysed sucrose rapidly and simultaneously formed fructooligosaccharides by transfructosylation. | 1991 | 24425020 |
phytochemical contents and antioxidant capacities of different parts of two sugarcane (saccharum officinarum l.) cultivars. | antioxidant capacities and phytochemicals such as phenolics, flavonoids, triterpenoids, phytosterols of four parts of two sugarcane cultivars have been characterised. the total triterpenoid content (ttc), total phenolic content (tpc), total flavonoid content (tfc) and total sterol content (tsc) were different among parts and cultivars of sugarcane. the node of both cultivars contained the highest ttc (2096.02 and 1779.66 mg ursolic acid/100g dw for green-rind and red-rind sugarcane, respectively ... | 2014 | 24423556 |
biosynthesis of invertase by saccharomyces cerevisiae with sugarcane molasses as substrate. | biosynthesis of invertase by saccharomyces cerevisiae 01k32 was inversely proportional to the concentration of sugarcane blackstrap molasses included in the medium. in a fermenter, an intracellular invertase activity of 440 u/g dry cells was obtained. | 1993 | 24420295 |
production of α-galactosidase by monascus grown on soybean and sugarcane wastes. | extracts of both sugarcane and soybean wastes supported the growth of monascus but sugarcane waste was superior for the production of α-galactosidase. an aqueous extract prepared from 5% (w/v) soybean waste and 7% (w/v) sugarcane waste gave the best result and was superior to the standard peptone/glucose/yeast extract medium. liquid-solid mixtures were slightly less effective. enzyme production could be enhanced by adding raffinose. enzymatic hydrolysis of p-nitrophenyl-α-d-galactoside was optim ... | 1993 | 24420194 |
sugarcane-pressmud as a novel substrate for production of citric acid by solid-state fermentation. | sugarcane-pressmud, a by-product of cane-sugar manufacture, was used as a substrate for production of citric acid by aspergillus niger cftri 30, in a solid-state fermentation system. of the 170 g of sugar supplied, 131 g were consumed, with a 79% yield of citric acid over 120 h. potassium ferrocyanide improved the conversion to about 88% and lowered the fermentation time by 24 h. enrichment with sugar and nh4no3 was essential to improve productivity. about 174 g citric acid/kg dry sugarcane-pres ... | 1993 | 24420047 |
genomic analysis of xanthomonas translucens pathogenic on wheat and barley reveals cross-kingdom gene transfer events and diverse protein delivery systems. | in comparison to dicot-infecting bacteria, only limited numbers of genome sequences are available for monocot-infecting and in particular cereal-infecting bacteria. herein we report the characterisation and genome sequence of xanthomonas translucens isolate dar61454 pathogenic on wheat and barley. based on phylogenetic analysis of the atp synthase beta subunit (atpd) gene, dar61454 is most closely related to other x. translucens strains and the sugarcane- and banana- infecting xanthomonas strain ... | 2014 | 24416331 |
draft genome sequence of atrazine-utilizing bacteria isolated from indian agricultural soil. | we report the draft genome sequences of two tropical bacterial isolates capable of degrading the herbicide atrazine. alcaligenes sp. strain egd-ak7 and arthrobacter sp. strain ak-yn10 were isolated from indian agricultural soil in which sugarcane is grown, with a reported history of atrazine use. egd-ak7 has the atzabcdef genes and ak-yn10 has the trzn and atzbc genes for atrazine degradation. | 2014 | 24407646 |
finished genome of zymomonas mobilis subsp. mobilis strain cp4, an applied ethanol producer. | zymomonas mobilis subsp. mobilis is one of the most rigorous ethanol-producing organisms known to date, considered by many to be the prokaryotic alternative to yeast. the two most applied z. mobilis subsp. mobilis strains, zm4 and cp4, derive from recife, brazil, and have been isolated from sugarcane fermentations. of these, zm4 was the first z. mobilis representative strain to be sequenced and analyzed. here, we report the finishing of the genome sequence of strain cp4, which is highly similar ... | 2014 | 24407627 |
genome characterization of sugarcane yellow leaf virus from china reveals a novel recombinant genotype. | sugarcane yellow leaf virus (scylv; genus polerovirus, family luteoviridae) is a recombinant virus associated with yellow leaf disease, a serious threat to sugarcane in china and worldwide. among the nine known scylv genotypes existing worldwide, col, haw, reu, ind, chn1, chn2, bra, cub and per, the last five have been reported in china. in this study, the complete genome sequences (5,880 nt) of gz-gz18 and hn-cp502 isolates from the chinese provinces of guizhou and hainan, respectively, were cl ... | 2014 | 24395076 |
isolation of a novel peroxisomal catalase gene from sugarcane, which is responsive to biotic and abiotic stresses. | catalase is an iron porphyrin enzyme, which serves as an efficient scavenger of reactive oxygen species (ros) to avoid oxidative damage. in sugarcane, the enzymatic activity of catalase in a variety (yacheng05-179) resistant to the smut pathogen sporisorium scitamineum was always higher than that of the susceptible variety (liucheng03-182), suggesting that catalase activity may have a positive correlation with smut resistance in sugarcane. to understand the function of catalase at the molecular ... | 2014 | 24392135 |