Publications

morphological compatibility of white clover and perennial ryegrass cultivars grown under two nitrate levels in flowing solution culture.	the effects of nitrate (no3-) supply on shoot morphology, vertical distribution of shoot and root biomass and total nitrogen (n) acquisition by two perennial ryegrass (lolium perenne l.) cultivars (aberelan and preference) and two white clover (trifolium repens l.) cultivars (grasslands huia and aberherald) were studied in flowing nutrient culture. cultivars were grown from seed as monocultures and the clovers inoculated with rhizobium. the 6-week measurement period began on day 34 (grasses) and ...	2003	12876188
effects of earthworms and organic litter distribution on plant performance and aphid reproduction.	human management practices and large detritivores such as earthworms incorporate plant litter into the soil, thereby forming a heterogeneous soil environment from which plant roots extract nutrients. in a greenhouse experiment we investigated effects of earthworms and spatial distribution of (15)n-labelled grass litter on plants of different functional groups [ lolium perenne (grass), plantago lanceolata (forb), trifolium repens (legume)]. earthworms enhanced shoot and root growth in l. perenne ...	2003	12844255
[legume cross-reactivity].	legumes are dicotyledonous plants belonging to the fabales order. the main distinctive characteristic of which is their fruit (legumen, seeds contained in pods). this botanical order is formed by three families: mimosaceae, caesalpiniaceae and papilionaceae or fabacea. the papilionaceae family includes the most important allergenic species: lens culinaris (lentil), cicer arietinum (chick-pea), pisum sátivum (pea), arachis hipogea (peanut), phaseolus vulgaris (bean) y glycine max (soy). legumes a ...	2003	12783766
diversity of arbuscular mycorrhizal fungi colonising roots of the grass species agrostis capillaris and lolium perenne in a field experiment.	analysis of arbuscular mycorrhizal (am) fungal diversity through morphological characters of spores and intraradicular hyphae has suggested previously that preferential associations occur between plants and am fungi. a field experiment was established to investigate whether am fungal diversity is affected by different host plants in upland grasslands. indigenous vegetation from plots in an unimproved pasture was replaced with monocultures of either agrostis capillaris or lolium perenne. modifica ...	2004	12768382
epidemiology of atopy in 220 children. diagnostic reliability of skin prick tests and total and specific ige levels.	we have prospectively studied 220 children attending our division because they suffered from atopic dermatitis (ad), asthma, and allergic rhinitis (ar), to assess the epidemiology of atopic diseases, and effectiveness of the diagnostic tests commonly used in allergic children.	2003	12754457
carbon mineralisation and plant growth in soil amended with compost samples at different degrees of maturity.	the carbon and nitrogen mineralisation of a composting mixture of brewing yeast and lemon tree prunings was studied, at different degrees of stabilisation of this matrix, within an incubation experiment in soil. meanwhile, a growth test in pots with ryegrass (lolium perenne l.) was carried out using the selected soil and equal amounts of the composting mixture taken at different maturation steps, in order to evaluate the additions of these organic amendments in terms of fertilising value. sample ...	2003	12739731
impact of land use intensity on the species diversity of arbuscular mycorrhizal fungi in agroecosystems of central europe.	the impact of land use intensity on the diversity of arbuscular mycorrhizal fungi (amf) was investigated at eight sites in the "three-country corner" of france, germany, and switzerland. three sites were low-input, species-rich grasslands. two sites represented low- to moderate-input farming with a 7-year crop rotation, and three sites represented high-input continuous maize monocropping. representative soil samples were taken, and the amf spores present were morphologically identified and count ...	2003	12732553
surveys of microfungi in a former industrial area in duisburg-nord.	one hundred and forty microfungi (ascomycetes and deuteromycetes) were collected in the "landschaftspark duisburg-nord" located in north rhine-westphalia. new hosts for rarely found fungi are recorded for the first time. new for germany are massaria inquinans (tode) de not. and nitschkia grevillei (rhem) nannf. on acer pseudoplatanus l., pirottaea nigrostriata graddon on artemisia vulgaris l., ceratopycnis clematidis höhn. on clematis vitalba l., dasyscyphus aff. humuli (w. phillips) dennis on h ...	2002	12701427
the development of trichostrongylus colubriformis larvae on a range of herbage species or on plots of differing topographical aspect.	five "contaminations", where faeces containing trichostrongylus colubriformis eggs were deposited on pasture and serially recovered, were used to compare the rate of decline of faecal mass and larval development. in the first three contaminations, faeces from a common source were deposited on swards of browntop (agrostis capillaris cv grasslands muster), ryegrass (lolium perenne cv grassland nui), white clover (trifolium pratense cv grassland tahora), or onto bare ground in the late spring, summ ...	2003	12591198
the effect of birdsfoot trefoil (lotus corniculatus) and chicory (cichorium intybus) on parasite intensities and performance of lambs naturally infected with helminth parasites.	conventionally, farmers rely upon the routine use of anthelmintics to control helminth parasites and their use has proved highly cost-effective. however, several factors, including the emergence of helminths resistant to pharmaceutical anthelmintics, are forcing farmers to seek alternative approaches to parasite control. studies in new zealand have shown that some alternative forages may reduce parasitic infestation in sheep. in the current study, it was found that under uk environmental conditi ...	2003	12581592
the physico-chemical characterization of a boiling stable antifreeze protein from a perennial grass (lolium perenne).	we have characterized a cold-induced, boiling stable antifreeze protein. this highly active ice recrystallization inhibition protein shows a much lower thermal hysteresis effect and displays binding behavior that is uncharacteristic of any afp from fish or insects. ice-binding studies show it binds to the (1 0 1 0) plane of ice and ftir studies reveal that it has an unusual type of highly beta-sheeted secondary structure. ice-binding studies of both glycosylated and nonglycosylated expressed for ...	2003	12573283
[responses of agricultural crops of free-air co2 enrichment].	over the past decade, free-air co2 enrichment (face) experiments have been conducted on several agricultural crops: wheat(triticum aestivum l.), perennial ryegrass (lolium perenne), and rice(oryza sativa l.) which are c3 grasses; sorghum (sorghum bicolor (l.) möench), a c4 grass; white clover (trifolium repens), a c3 legume; potato (solanum tuberosum l.), a c3 forb with tuber storage; and cotton (gossypium hirsutum l.) and grape (vitis vinifera l.) which are c3 woody perennials. using reports fr ...	2002	12557686
feed intake of young double-muscled bulls fed on grass and supplemented with sugar-beet pulp.	daily dry matter intake in young growing double-muscled bulls, fed indoors on grass, was estimated based on forty-four intake data from 28 animals, ageing at least five months and weighing up to 400 kg live weight. intake was measured during five consecutive days using one of eighteen cuts of grass. fresh meadow grass (mainly lolium perenne) was fed ad libitum and two kg dried sugar-beet pulp was offered per animal and per day. animal live weight averaged 278 +/- 82 kg and mean total daily dry m ...	2002	12556046
morphological variation in different host populations of abacarus hystrix (acari: prostigmata: eriophyoidea).	the present systematic classification of eriophyoidea is widely recognised as artificial and not reflecting plant-herbivore linkage. quantitative description of host-related morphological variation can provide the basic information needed to improve the eriophyoid taxonomic system and enhance our understanding of mechanisms generating this variation. the purpose of this study was to investigate quantitative morphological traits of populations of cereal rust mite abacarus hystrix (nalepa 1896) li ...	2002	12537292
lotus corniculatus condensed tannins decrease in vivo populations of proteolytic bacteria and affect nitrogen metabolism in the rumen of sheep.	condensed tannins in forage legumes improve the nutrition of sheep by reducing ruminal degradation of plant protein and increasing crude protein flow to the intestine. however, the effects of condensed tannins in forage legumes on rumen bacterial populations in vivo are poorly understood. the aim of this study was to investigate the specific effects of condensed tannins from lotus corniculatus on four proteolytic rumen bacteria in sheep during and after transition from a ryegrass (lolium perenne ...	2002	12489781
alpha-tocopherol concentrations and case life of lamb muscle as influenced by concentrate or pasture finishing.	two experiments were conducted to evaluate alpha-tocopherol accumulation in muscle of lambs finished on pasture or concentrates. the objective for exp. 1 was to compare accumulation of alpha-tocopherol in the longissimus muscle of pasture-fed lambs to that of lambs fed three concentrations (15, 150, and 300 iu/kg of dm) of supplemental vitamin e (all rac alpha-tocopheryl acetate) in all-concentrate diets. the objective in exp. 2 was to investigate the effect of duration of supplemental vitamin e ...	2002	12413072
[allergens and risk factors in pediatric patients with allergic seasonal conjunctivitis].	the seasonal allergic conjunctivitis is an inflammatory disease of the ocular surface that affects mainly children, with predominance of male sex. it is an immunological disease with a typical reaction of hypersensitivity type 1 (ige), resulted from several biological reactions (antigen-antibody) and it's associated to several risk factors.	2002	12374042
effects of grazing undrenched weaner deer on chicory or perennial ryegrass/white clover pasture on the viability of gastrointestinal nematodes and lungworms.	this study determined the in vitro effects on the viability of internal parasites of grazing undrenched weaner deer on either chicory (cichorium intybus) or perennial ryegrass (lolium perenne)/white clover (trifolium repens) pasture. one experiment investigated the hatching and development of gastrointestinal nematode eggs and larvae, and the development and motility of l1 lungworm (dictyocaulus eckerti) larvae, and a second experiment used larval migration inhibition assays to test the viabilit ...	2002	12371691
a novel fungal protease expressed in endophytic infection of poa species.	the fungus acremonium typhinum produces a novel endoprotease during symbiotic endophytic infection of the grass, poa ampla. this protease is unusual because it is highly active in the presence of sodium dodecyl sulfate. the enzyme is a thiol-containing serine protease and is localized to a crude membrane fraction. similar protease activity has been detected in endophyte-infected poa autumnalis and poa sylvestris plants. expression of this protease may be important in endophytic infection of poa ...	1993	12231854
is lolium pollen from an urban environment more allergenic than rural pollen?	allergy to grass pollen is a highly prevalent allergic disease. hay fever is more predominant in urban than in rural areas, despite the increasingly smaller areas of surrounding grassland. the effect of vehicle exhaust pollutants, mainly diesel particles, and other industrial sources of atmospheric pollution leading to plant damage has been implicated in this phenomenon.	2002	12199966
clinical characteristics of patients with mustard hypersensitivity.	although mustard is frequently consumed in spain and elsewhere, only isolated case reports of mustard allergy have been reported. no large series of case studies have been published.	2002	12197573
attenuation of groundwater contamination caused by cattle slurry: a plot-scale experimental study.	infiltration of contaminants was investigated in a flat pasture plot lolium perenne l. which received 250 m3/ha of cattle slurry. lysimeters and piezometers had previously been installed in the plot to sample groundwater at different depths. water samples were analysed for ph, conductivity, nh4(+), no3-, orthophosphate, cl-, na+, k+, ca2+, mg2+ and chemical oxygen demand (cod), and for faecal coliforms and faecal streptococci. contaminant concentrations in water samples taken in lysimeters at a ...	2002	12139326
phytoremediation of subarctic soil contaminated with diesel fuel.	the effects of several plant species, native to northern latitudes, and different soil amendments, on diesel fuel removal from soil were studied. plant treatments included scots pine (pinus sylvestris), poplar (populus deltoides x wettsteinii), a grass mixture (red fescue, fesuca rubra; smooth meadowgrass, poa pratensis and perennial ryegrass, lolium perenne) and a legume mixture (white clover, trifolium repens and pea, pisum sativum). soil amendments included npk fertiliser, a compost extract a ...	2002	12118697
does decreased mowing frequency enhance alkaloid production in endophytic tall fescue and perennial ryegrass?	tall fescue, festuca arundinacea, and perennial ryegrass, lolium perenne. are widely infected with fungal endophytes (neotyphodium spp.). the symbiosis between plant and fungus leads to synthesis of alkaloids that have been shown to be either toxic or act as feeding deterrents against insect pests. as cultural practices have the potential to regulate production of plant secondary metabolites, we evaluated the influence of mowing frequency on the levels of major alkaloids in tall fescue and peren ...	2002	12049232
degradation of fresh ryegrass by methanogenic co-cultures of ruminal fungi grown in the presence or absence of fibrobacter succinogenes.	the ability of five ruminal fungi in syntrophic co-culture with the methanogen methanobrevibacter smithii to degrade perennial ryegrass ( lolium perenne) stem fragments and leaf blades was studied to determine the susceptibilities of non-autoclaved fresh tissues to fungal degradation. autoclaving did not significantly increase fungal degradation of stem fragments but strongly increased degradation of leaf blades by a species of caecomyces. in methanogenic co-cultures, non-autoclaved stem fragmen ...	2002	12029527
grass species and endophyte effects on survival and development of fall armyworm (lepidoptera: noctuidae).	grass selections including 10 zoysiagrasses, 18 paspalums, 34 bermuda grasses, tall fescue, creeping red fescue, and perennial ryegrasses with and without endophyte were evaluated for potential resistance to fall armyworm, spodoptera frugiperda (j. e. smith), larvae. laboratory evaluations assessed the degree of antibiosis among >70 grass lines to first-instar fall armvworms. when all parameters measured were considered, the trend in resistance to fall armyworm among endophyte-infected (e+) and ...	2002	12020031
influence of flavor on goat feeding preferences.	goat feeding preferences for straw pellets flavored with ryegrass (lolium perenne, cv. belida) or white clover (trifolium repens, cv. huia) aromatic extracts, obtained by means of a cold aromatic extraction method, were assessed with cafeteria trials. prior to the trials, odor differences between the two plant species, the two aromatic extracts, and the straw pellets sprayed with the two aromatic extracts were verified using sensory analysis performed by 30 human panelists. since odor difference ...	2002	11925067
[the effects of the primary growth of two ryegrasses on the dynamics of changes in digestibility and feed intake by sheep].	dynamics of changes in digestibility and feed intake by sheep of two ryegrass species during primary growth environmental impacts can cause short-term variations in chemical composition and feeding value of grass swards during growth and maturation and induce concomitant changes of intake of these grasses by animals. continuous digestion trials are effective in observing the dynamics of these variations. therefore this study was conducted to evaluate the effects of maturity-related alterations i ...	2001	11906563
dynamic and steady-state responses of inorganic nitrogen pools and nh(3) exchange in leaves of lolium perenne and bromus erectus to changes in root nitrogen supply.	short- and long-term responses of inorganic n pools and plant-atmosphere nh(3) exchange to changes in external n supply were investigated in 11-week-old plants of two grass species, lolium perenne and bromus erectus, characteristic of n-rich and n-poor grassland ecosystems, respectively. a switch of root n source from no(-)(3)to nh(4)(+) caused within 3 h a 3- to 6-fold increase in leaf apoplastic nh(4)(+) concentration and a simultaneous decrease in apoplastic ph of about 0.4 ph units in both s ...	2002	11842177
accumulation of hmx (octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine) in indigenous and agricultural plants grown in hmx-contaminated anti-tank firing-range soil.	to investigate their potential for phytoremediation, selected agricultural and indigenous terrestrial plants were examined fortheir capacity to accumulate and degrade the explosive octahydro-1 ,3,5,7-tetra nitro-1,3,5,7-tetrazocine (hmx). plant tissue and soil extracts were analyzed for the presence of hmx and possible degradative metabolites using high-performance liquid chromatography with diode-array uv detection (hplc-uv), micellar electrokinetic chromatography with diode-array uv detection ...	2002	11811476
ryegrass host genetic control of concentrations of endophyte-derived alkaloids.	endophytic fungi in pasture grasses produce alkaloids which affect invertebrate and vertebrate herbivores. while the competence to produce an alkaloid is a property of the fungus, the host plant may moderate fungal activity. host genetic influence on endophyte activity was studied in perennial ryegrass (lolium perenne l.) infected with a common strain of neotyphodium lolii (latch, christensen & samuels) glenn, bacon & hanlin. progeny seedling families of a partial diallel cross and their 12 pare ...	2002	11756253
in planta regulation of extension of an endophytic fungus and maintenance of high metabolic rates in its mycelium in the absence of apical extension.	the fungus neotyphodium lolii is an endophytic symbiont. it grows in the intercellular spaces of the perennial ryegrass lolium perenne, producing secondary metabolites which enhance the fitness of the association over that of uninfected l. perenne. we report that the average number of hyphal strands in a given section of a leaf remains constant during the life of a leaf, indicating synchrony of leaf and hyphal extension, including cessation of hyphal extension when leaf extension ceases. we used ...	2001	11722882
black cutworm (lepidoptera: noctuidae) larval emigration and biomass in mixtures of endophytic perennial ryegrass and kentucky bluegrass.	studies examined the possibility that mixtures of endophytic perennial ryegrass and kentucky bluegrass provide resistance against larvae of the black cutworm, agrotis ipsilon (hufnagel). we hypothesized that resistance against a. ipsilon in such stands would stem from the influence of kentucky bluegrass on a. ipsilon growth and behavior rather than the influence of endophytic perennial rvegrass. in replicated greenhouse experiments, black cutworm larvae initially emigrated more quickly from pots ...	2001	11681682
detection of endophyte toxins in the imported perennial ryegrass straw.	from 1997 to 1999, 29 cases of disorders were detected in cattle and horses that had been fed ryegrass straw imported from the u.s.a. these animals showed symptoms resembling ryegrass staggers and the clinical signs disappeared after removal of the straw. endophytic hyphae were detected in the seeds of all straw samples that were responsible for the clinical cases. lolitrem b concentrations in the straw ranged between 972 and 3740 ppb. ergovaline concentrations were between 355 and 1300 ppb. eve ...	2001	11642270
elimination of ergovaline from a grass-neotyphodium endophyte symbiosis by genetic modification of the endophyte.	the fungal endophytes neotyphodium lolii and neotyphodium sp. lp1 from perennial ryegrass (lolium perenne), and related endophytes in other grasses, produce the ergopeptine toxin ergovaline, among other alkaloids, while also increasing plant fitness and resistance to biotic and abiotic stress. in the related fungus, claviceps purpurea, the biosynthesis of ergopeptines requires the activities of two peptide synthetases, lps1 and lps2. a peptide synthetase gene hypothesized to be important for erg ...	2001	11592979
surface runoff pollution by cattle slurry and inorganic fertilizer spreading: chemical oxygen demand, ortho-phosphates, and electrical conductivity levels for different buffer strip lengths.	as a way of dealing with the removal of pollutants from farming practices generated wastewater in the eu, we investigate the effect of spreading cattle slurry and inorganic fertiliser on 8 x 5 m2 and 8 x 3 m2 areas, referred to surface runoff chemical oxygen demand (cod), ortho-phosphates (o-p) and electrical conductivity (ec) levels, and the efficiency of grass buffer strips of various lengths in removing pollutants from runoff. the experimental plot was a 15% sloped lolium perenne pasture. sur ...	2001	11496670
allergen-induced basophil activation: cd63 cell expression detected by flow cytometry in patients allergic to dermatophagoides pteronyssinus and lolium perenne.	in this study, we determined by flow cytometry the percentage of basophils activated after in vitro stimulation by allergens and expressing the cd63 marker. the diagnostic reliability of the technique was assessed as well as its correlation with other in vitro diagnostic parameters.	2001	11467990
adaptation of ruminococcus flavefaciens resulting in increased degradation of ryegrass cell walls.	this study investigated the long term adaptation of a ruminal bacterium to growth on four different plant cell wall substrates. no significant increase in degradation was detected for lucerne, barley straw or weeping lovegrass after 23 serial subcultures of the cellulolytic rumen bacterium ruminococcus flavefaciens strain 17 on each of these substrates. significantly increased substrate degradation by r. flavefaciens strain 17 was however observed after 23 subcultures on perennial ryegrass. the ...	2001	11451517
release of allergen-bearing cytoplasm from hydrated pollen: a mechanism common to a variety of grass (poaceae) species revealed by electron microscopy.	the release of submicronic particles from grass pollen after rainfall was suggested to be responsible for outbreaks of grass pollen asthma. recently, we provided evidence for the release of respirable allergen-bearing particles from hydrated ryegrass (lolium perenne ) pollen as a possible explanation for this phenomenon.	2001	11447390
correlation of endophyte toxins (ergovaline and lolitrem b) with clinical disease: fescue foot and perennial ryegrass staggers.	fescue foot, summer syndrome, reproductive problems, and ryegrass staggers are all diseases of livestock related to endophyte toxins in pasture grasses. range finding experiments and case studies of fescue foot relative to ergovaline toxin found in endophyte infected tall fescue and lolitrem b present in endophyte infected perennial ryegrass were conducted. within 42 d of initiating a feeding trial with chopped tall fescue straw containing 825 ppb ergovaline and at environmental temperatures of ...	2001	11383653
association between feeding perennial ryegrass (lolium perenne cultivar grasslands impact) containing high concentrations of ergovaline, and health and productivity in a herd of lactating dairy cows.	perennial ryegrasses are frequently infected with fungal endophyte (neotyphodium lolii) to increase the resistance of the plant to insect damage. unfortunately, a side effect of endophyte infection can be the production of alkaloids, including lolitrem b and ergovaline, that produce toxic effects in animals. a significant 4.6 litre decrease in milk production in a herd of holstein-friesian dairy cows was associated with high concentrations of ergovaline in ryegrass silage. simultaneously, milk s ...	2001	11349413
a comparison of manometric and membrane-electrode procedures for measuring rates of leaf decay.	two instruments were compared for consistency of decay-rate measurements of four leaf types: lolium perenne, typha domingenesis, casuarina cunninghamiana and eucalyptus polyanthemos, via progressive biological oxygen demand (bod). the instruments were (a) a dissolved oxygen probe (i.e. an oxygen-selective membrane electrode) used in two different modes: (1) measurements of sacrificial samples that were successively discarded, and (2) repeated measurements of the same sample, and (b) a mercury fr ...	2001	11349381
anaerobically digested poultry slaughterhouse wastes as fertiliser in agriculture.	chemical and physical analysis, 27-d plant growth assays with carrot (daucus carota) and chinese cabbage (brassica campestris var. chinensis), and 5-d phytotoxicity assays with chinese cabbage and perennial ryegrass (lolium perenne) were used to investigate the suitability of anaerobically digested poultry slaughterhouse waste for fertiliser in agriculture and the effect of aerobic post-treatment on the properties of the digested material. the digested material appeared to be rich in nitrogen. i ...	2001	11265792
a terminal flower1-like gene from perennial ryegrass involved in floral transition and axillary meristem identity.	control of flowering and the regulation of plant architecture have been thoroughly investigated in a number of well-studied dicot plants such as arabidopsis, antirrhinum, and tobacco. however, in many important monocot seed crops, molecular information on plant reproduction is still limited. to investigate the regulation of meristem identity and the control of floral transition in perennial ryegrass (lolium perenne) we isolated a ryegrass terminal flower1-like gene, lptfl1, and characterized it ...	2001	11244130
predicting the transfer of radiocaesium from organic soils to plants using soil characteristics.	a model predicting plant uptake of radiocaesium based on soil characteristics is described. three soil parameters required to determine radiocaesium bioavailability in soils are estimated in the model: the labile caesium distribution coefficient (kd1), k+ concentration in the soil solution [mk] and the soil solution-->plant radiocaesium concentration factor (cf, bq kg-1 plant/bq dm-3). these were determined as functions of soil clay content, exchangeable k+ status, ph, nh4+ concentration and org ...	2001	11202684
the effect of applying sodium fertilizer on the rate of digestion of perennial ryegrass and white clover incubated in rumen liquor, with implications for ruminal tympany in cattle.	a high herbage k:na ratio increases the risk of ruminal tympany in cattle, which may relate to digestion rate. experiment 1 examined whether in vitro digestibility of ryegrass was affected by nacl fertilizer or by na concentration in artificial saliva. fertilizer na increased grass digestibility, but na in artificial saliva decreased it, probably due to the energy cost of sodium exclusion from bacteria. increased herbage digestibility with fertilizer na is therefore not due to additional na, but ...	2001	11145830
overseeding endophytic perennial ryegrass into stands of kentucky bluegrass to manage bluegrass billbug (coleoptera: curculionidae).	studies were conducted to determine the utility of overseeding endophyte enhanced varieties of perennial ryegrass, lolium perenne l., into existing stands of kentucky bluegrass, poa pratensis l., to manage bluegrass billbug, sphenophorus parvulus gyllenhal, larval populations and their damage. two commercial varieties of endophyte enhanced perennial ryegrass (repell ii and triple play) were overseeded at two different rates in the field. s. parvulus larval populations, their damage, and turfgras ...	2000	11142296
cloning and expression of a distinct subclass of plant thioredoxins.	mrnas encoding a novel thioredoxin were isolated from pollen rna of lolium perenne (lptrx), hordeum bulbosum (hbtrx), phalaris coerulescens (ptrx) and secale cereale (sctrx). the cdnas contain a single orf of 393 bp encoding a protein of 131 amino acids. the predicted proteins showed highest homology to plant thioredoxins of the h class yet form a distinct subgroup that is characterized by a high level of sequence conservation (95.4-97.7% identity). genbank searches revealed additional members o ...	2000	11106422
portal net appearance of amino acids in growing pigs fed a barley-based diet with inclusion of three different forage meals.	the net absorption of amino acids (aa) in young pigs fed a barley-based control diet (c) and diets where barley was replaced by 200 g/kg fresh weight of dried lucerne (medicago sativa; l20), white clover (trifolium repens; w20) or perennial ryegrass (lolium perenne; pr20) meal was studied. castrated male pigs were fitted with permanent catheters in the hepatic portal vein and mesenteric artery, and the hepatic portal net absorption of aa was estimated from the porto-arterial plasma concentration ...	2000	11103219
response of a soil bacterial community to grassland succession as monitored by 16s rrna levels of the predominant ribotypes.	the composition of predominant soil bacteria during grassland succession was investigated in the dutch drentse a area. five meadows, taken out of agricultural production at different time points, and one currently fertilized plot represented different stages of grassland succession. since fertilization and agricultural production were stopped, the six plots showed a constant decline in the levels of nutrients and vegetation changes. the activity of the predominant bacteria was monitored by direc ...	2000	10966420
effects of zinc and influence of acremonium lolii on growth parameters, chlorophyll a fluorescence and antioxidant enzyme activities of ryegrass (lolium perenne l. cv apollo).	the effects of zinc on growth, mineral content, chlorophyll a fluorescence, and detoxifying enzyme activity (ascorbate peroxidase (apx), ec 1.11.1.11; superoxide dismutase (sod), ec 1.15.1.1) of ryegrass infected or not by acremonium lolii, and treated with nutrient solution containing 0-50 mm znso(4) were studied. the introduction of zinc induces stress with a decrease in growth at 1, 5 and 10 mm znso(4) and a cessation of growth at 50 mm znso(4), in ryegrass plants infected by a. lolii or not. ...	2000	10948221
amino acids as a nitrogen source in temperate upland grasslands: the use of dual labelled ((13)c, (15)n) glycine to test for direct uptake by dominant grasses.	it is becoming increasingly apparent that soil amino acids are a principal source of nitrogen (n) for certain plants, and especially those of n-limited environments. this study of temperate upland grasslands used glycine-2-(13)c-(15)n and ((15)nh4)(2)so(4) labelling techniques to test the hypothesis that plant species which dominate 'unimproved' semi-natural grasslands (festuca-agrostis-galium) are able to utilise amino acid n for growth, whereas those plants which dominate 'improved' grasslands ...	2000	10920354
effect of breed and age on the voluntary intake and the micromineral status of non-pregnant sheep. ii. micromineral status.	twenty-four non-pregnant sheep, divided into four groups of six animals each, were used to test breed (suffolk and rambouillet) and age (mature (six years old) and yearling (16 months old) ewes) differences in micromineral status. the animals grazed alternatively two mixed pastures containing trifolium repens latum, lolium perenne and pennisetum clandestinum from 07:00 to 16:30hours, and remained penned overnight. hand-plucked forage samples, blood and wool samples were collected on days 1 (exce ...	2000	10867321
effect of breed and age on the voluntary intake and the micromineral status of non-pregnant sheep. 1. estimation of voluntary intake.	twenty-four sheep were used to test breed and age differences in voluntary intake (vi). the sheep were divided into four groups of six animals each: suffolk mature ewes (sm), suffolk yearlings (sy), rambouillet mature ewes (rm), and rambouillet yearlings (ry). the animals grazed alternatively two mixed pastures containing trifolium repens latum, lolium perenne and pennisetum clandestinum from 07:00 to 16:30 hours and remained penned overnight. voluntary intake was estimated using chromic sesquio ...	2000	10867320
expulsion of allergen-containing materials from hydrated rye grass (lolium perenne) pollen revealed by using immunogold field emission scanning and transmission electron microscopy.	several studies demonstrated episodes of grass pollen-induced allergic asthma after heavy rainfalls. it has been hypothesized that these asthma attacks might be due to the release of respirable allergen-bearing particles from pollen cytoplasm.	2000	10856148
lawn parameters influencing abundance and distribution of the hairy chinch bug (hemiptera: lygaeidae).	management of lawns that promotes conditions detrimental to the development of insect pests may represent a valuable environmentally benign turfgrass management strategy. in the cool-humid region of quebec, canada, we investigated 45 lawns infested with hairy chinch bug, blissus leucopterus hirtus montandon, to identify lawn parameters related to its distribution and abundance. kentucky bluegrass, creeping bentgrass, and perennial ryegrass, respectively, accounted for 55.8, 19.6, and 9.3% of the ...	2000	10826187
duration of regrowth of ryegrass (lolium perenne) effects on grazing behavior, intake, rumen fill, and fermentation of lactating dairy cows.	the relative importance of duration of sward regrowth and fill and fermentation in the rumen on the control of grazing time and intake rate during the first grazing session of the day was studied. four lactating dairy cows were allowed to graze ryegrass (lolium perenne) swards, with five different regrowth periods after mowing (6, 9, 16, 22, and 30 d). the cows were allowed to graze until they stopped voluntarily (cessation of grazing activity for at least 15 min). before and after grazing the r ...	2000	10821574
synthetic zeolites as amendments for sewage sludge-based compost.	the effects of incorporating a synthetic zeolite (zeolite p) in a range of concentrations (0.1-1.0 w:w) into an experimental horticultural compost, derived from sewage sludge, have been investigated. the impact of zeolite treatment on time-related changes of the labile zinc, copper, iron and manganese pools within the compost was compared to lime incorporation (5% w:w) and to a proprietary unamended peat-based compost. addition of 0.5% and 1.0% zeolite significantly reduced labile zinc over a 90 ...	2000	10819210
histamine receptor expression on peripheral blood lymphocytes is influenced by specific and nonspecific activation.	histamine is a physiological mediator which exerts both effector and regulatory functions through its receptors on various cells. the aim of the study was to investigate changes in histamine receptor expression on peripheral blood lymphocytes affected by stimulation with both specific and nonspecific stimuli. lymphocytes were obtained from both healthy and allergic subjects. cells were incubated with various allergens (mixed grass pollen, lolium perenne, dermatophagoides pteronyssinus 1, bee ven ...	2000	10807051
ileal amino acid digestibilities in pigs of barley-based diets with inclusion of lucerne (medicago sativa), white clover (trifolium repens), red clover (trifolium pratense) or perennial ryegrass (lolium perenne).	two experiments were performed with post-valve t-cannulated growing pigs, using five animals in each experiment in a change-over design to evaluate the effect of inclusion of four different dried forage meals on ileal crude protein (cp) and amino acid (aa) digestibilities. the control diets (c1 and c2) were barley-based and the experimental diets were formulated by replacing the barley with 100 or 200 g/kg of either lucerne (medicago sativa) or white clover (trifolium repens) meal in expt 1 and ...	1999	10743486
use of length heterogeneity pcr and fatty acid methyl ester profiles to characterize microbial communities in soil.	in length heterogeneity pcr (lh-pcr) a fluorescently labeled primer is used to determine the relative amounts of amplified sequences originating from different microorganisms. labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene sequencer. we used lh-pcr to evaluate the composition of the soil microbial community. four soils, which differed in terms of soil type and/or crop management practice, were studied. previous data for mi ...	2000	10742258
substrate preference profiles of proteases released by allergenic pollens.	pollens are important triggers for allergic asthma and seasonal rhinitis. we have recently reported that proteases released by major allergenic pollens can injure airway epithelial cells in vitro. disruption of epithelial integrity by proteases released following deposition of pollens on mucosal surfaces could promote sensitization and induce inflammation.	2000	10718856
quantitative assessment of in planta distribution of metabolic activity and gene expression of an endophytic fungus.	using perennial ryegrass infected with an acremonium transformant carrying the escherichia coli beta-d-glucuronidase gene (gusa) (gus system) under control of a constitutive promoter, we have developed methods for the quantitative extraction of endophyte-associated gus activity from plant material. fluorometric assays of these extracts allow quantitative assessment of the distribution of endophyte-associated gus activity within single plants (tillers) with high resolution. fluorescence microscop ...	1997	10627142
efficiency of some in vitro allergy tests for evaluating atopy in children and adults.	in recent years, an increase in the prevalence of allergic diseases has been reported, in industrialised countries. it is also a problem in non-industrialised countries, but its extent is less well documented. in this study, inhalant screening allergens (alatop), food screening allergens (fp5e), and total and specific ige were measured in 237 allergic patients (164 adults and 73 children) in order to examine the distribution of common allergens, the percentage of atopy/non-atopy among allergic c ...	1999	10616753
isolation of lolicine a, lolicine b, lolitriol, and lolitrem n from lolium perenne infected with neotyphodium lolii and evidence for the natural occurrence of 31-epilolitrem n and 31-epilolitrem f.	lolicines a (1a) and b (2a), late-eluting lolitrem-like compounds, were identified in extracts of perennial ryegrass (lolium perenne) seed that was infected with the endophytic fungus neotyphodium lolii. the lolicines were isolated as their 11-o-propionates (1b and 2b) and their structures determined by mass spectrometry and one- and two-dimensional nmr spectroscopy. the structures of lolicines a and b were similar to those of paspaline (5a) and paspaline b (5b), compounds known to be biosynthet ...	1998	10554283
terpendole m, a novel indole-diterpenoid isolated from lolium perenne infected with the endophytic fungus neotyphodium lolii.	terpendole m (1), a novel indole-diterpenoid, was isolated from perennial ryegrass (lolium perenne) infected with the endophytic fungus neotyphodium lolii. it was identified as 14alpha-hydroxyterpendole c by nmr and mass spectral techniques. the known indole-diterpenoids paspaline (3) and 13-desoxypaxilline (4) were also isolated from perennial ryegrass for the first time. terpendole m was less tremorgenic than terpendole c (2) in a standard mouse bioassay. these findings provide clues to the bi ...	1999	10552421
structural analysis of n-glycans from allergenic grass, ragweed and tree pollens: core alpha1,3-linked fucose and xylose present in all pollens examined.	the n-glycans from soluble extracts of ten pollens were examined. the pyridylaminated oligosaccharides derived from these sources were subject to gel filtration and reverse-phase hplc, in conjunction with exoglycosidase digests, and in some cases matrix-assisted laser desorption-ionisation mass spectrometry. in comparison to known structures, it was possible to determine the major structures of the n-glycans derived from kentucky blue grass (poa pratensis), rye (secale cerale), ryegrass (lolium ...	1998	10386890
influence of an elevated atmospheric co2 content on soil and rhizosphere bacterial communities beneath lolium perenne and trifolium repens under field conditions.	> abstract the increase in atmospheric co2 content alters c3 plant photosynthetic rate, leading to changes in rhizodeposition and other root activities. this may influence the activity, the biomass, and the structure of soil and rhizosphere microbial communities and therefore the nutrient cycling rates and the plant growth. the present paper focuses on bacterial numbers and on community structure. the rhizospheres of two grassland plants, lolium perenne (ryegrass) and trifolium repens (white clo ...	1999	10384008
differential recognition of recombinant phl p 5 isoallergens by phl p 5-specific t cells.	at present, recombinant allergens are considered for the diagnosis and treatment of atopic allergies. to evaluate the theoretical impact of isoallergen variation on the selection of isoallergens for specific allergy vaccination, we characterized the t-cell response of allergic patients to the phleum pratense major allergen, phl p 5, and five of its recombinant isoallergens.	1999	10224358
the kinetics of change in cytokine production by cd4 t cells during conventional allergen immunotherapy.	the effect of conventional allergen immunotherapy on allergen-specific t lymphocyte cytokine production is incompletely understood, particularly during the initial phase of treatment.	1999	10069882
production, faecal egg counts and worm burdens of ewe lambs which grazed six contrasting forages.	lambs were grazed on monospecific swards of one of six forages, some containing condensed tannins (ct); sulla (hedysarum coronarium), lotus corniculatus and l. pedunculatus and some without ct (lucerne (medicago sativa), plantain (plantago lanceolata) and a ryegrass/white clover pasture (lolium perenne/trifolium repens)) to evaluate effects of forage type on gastrointestinal nematode burdens, faecal egg count as well as liveweight gain (lwg), wool growth and dag formation over a 42-day period. a ...	1998	9877067
[specific skin reactions induced by individual pollen preparations in hypersensitivity persons].	skin tests are the most useful single modality for demonstrating an ige-mediated mechanism underlying clinical symptoms. skin reactivity to an allergen depends on person's exposure and genetic factors influencing the ige response. however, the reproducibility of allergy skin tests has been shown to be variable depending, among other things, on the allergen extract employed and the kind of technique used. these variations have led to controversy regarding clinical relevancy of allergy skin testin ...	2009	9863408
specific antigen targeting to surface ige and igg on mouse bone marrow-derived mast cells enhances efficiency of antigen presentation.	the discovery that bone marrow-derived mast cells can express major histocompatibility complex class ii molecules and act as antigen-presenting cells prompted us to evaluate this function when antigen is internalized through fluid-phase endocytosis or via specific uptake by using igg and ige antibodies. this study was performed using a specific t-cell hybridoma developed against lol p 1, the major allergen of grass pollen lolium perenne. expression of fc gamma r and fc epsilon ri by mast cells l ...	1998	9767412
determination of the uptake of [pt(nh3)4](no3)2 by grass cultivated on a sandy loam soil and by cucumber plants, grown hydroponically.	two cultivation experiments were carried out in order to answer the question to what extent platinum can enter the food chain by accumulation in plants, when the platinum is present in a bio-available form: (i) cucumber plants (cucumis sativus) were grown hydroponically in nutrient solutions containing [pt(nh3)4](no3)2 (from 0.5 to 50 micrograms pt/l solution); and (ii) a water-soluble platinum compound--[pt(nh3)4](no3)2--was added in increasing amounts to a sandy loam soil (from 0.5 to 50 mg pt ...	1998	9753790
molecular basis of ige-recognition of lol p 5, a major allergen of rye-grass pollen.	grass pollen, especially of rye-grass (lolium perenne). represents an important cause of type i allergy. identification of ige-binding (allergenic) epitopes of major grass pollen allergens is essential for understanding the molecular basis of interaction between allergens and human ige antibodies and therefore facilitates the devising of safer and more effective diagnostic and immunotherapy reagents. the aim of this study was to identify the allergenic epitopes of lol p 5, a major allergen of ry ...	1998	9747889
detection and quantification of group 4 allergens in grass pollen extracts using monoclonal antibodies.	grass pollen extracts are complex mixtures consisting of different major allergenic and non-allergenic components. phl p 4 is an important allergen, because more than 75% of grass pollen allergic patients produce specific ige antibodies against group 4 allergens.	1998	9720813
injury to murine airway epithelial cells by pollen enzymes.	pollens are important triggers for asthma but the mechanism of sensitisation to their proteins remains poorly understood. the intrinsic protease activity of some allergens may contribute to sensitisation by disrupting the integrity of the airway epithelial barrier. pollens release a variety of enzymes, including proteases, upon hydration. the hypothesis that such enzymes might be able to damage airway epithelial cells was therefore tested.	1998	9708228
growth, voluntary food intake and digestion in farmed temperate and tropical deer.	growth and voluntary feed intake (vfi) in grazing temperate farmed deer species are influenced by the feeding value of the forage and the stage of the deer's seasonal cycle. liveweight gain (lwg) of growing red deer was greater when perennial ryegrass (0.80)/white clover (0.20) pasture was grazed at 10 cm than 5 cm surface height, but venison production by one year of age was still low. chicory and red clover were of superior feeding value for deer than perennial ryegrass-based pastures, increas ...	1998	9704535
establishment and fecundity of ostertagia circumcincta and trichostrongylus colubriformis in lambs fed lotus (lotus pedunculatus) or perennial ryegrass.	forty four 12-14 week old polled dorset ram lambs, raised helminth free from birth, were used to investigate the effects of condensed tannins (ct) in lotus (lotus pedunculatus) on lamb growth and gastrointestinal nematode establishment and fecundity. condensed tannins bind to proteins in the rumen and increase the flow of protein to the intestines. lambs were allocated to either a ryegrass (lolium perenne) or lotus diet fed ad libitum, with or without twice daily drenching of polyethylene glycol ...	1998	9703616
specific ige to recombinant vegetal panallergen (rbet v 2) and fruit allergy in pollinic patients.	the presence of ige antibodies to the allergens rbet v 1 and rbet v 2 was investigated in patients with known sensitization to ryegrass (lolium perenne) and/or olive (olea europaea) pollen, by comparing a group of 10 patients who had allergic symptoms after ingestion of fruits (a) with a group of 17 patients who had only seasonal respiratory symptoms (b). there was no significant difference between the two groups for total ige. all patients showed specific ige to both l. perenne and o. europaea. ...	1998	9689344
molecular characterization of a cross-reactive juniperus oxycedrus pollen allergen, jun o 2: a novel calcium-binding allergen.	species belonging to the cupressaceae family are a relevant source of allergens that are present in a wide number of countries.	1998	9648704
limited genetic control of specific ige responses to rye grass pollen allergens in australian twins.	both genetic and environmental factors are thought to contribute to specific ige responses, however, the relative contribution of each in the responses to individual ryegrass pollen allergens is largely unknown even though some responses to allergens have been linked to certain hla complexes.	1998	9543082
ige antibodies to recombinant pollen allergens (phl p 1, phl p 2, phl p 5, and bet v 2) account for a high percentage of grass pollen-specific ige.	pollen from different grass species are some of the most potent elicitors of type i allergy worldwide. the characterization of antigenic structures and ige epitopes common to different grass species is relevant to define reagents for diagnosis and specific therapy of grass pollen allergy.	1998	9500760
dissection of the grass allergen-specific immune response in patients with allergies and control subjects: t-cell proliferation in patients does not correlate with specific serum ige and skin reactivity.	pollinosis, caused by grasses of the poaceae family, is a problem worldwide. the relative importance of grass groups 1 and 5 major allergens is well established. however, not much is known about the recognition of these allergens by t cells and whether this t-cell reactivity correlates with skin reactivity and serum ige levels.	1998	9500758
performance of lactating dairy cows fed alfalfa silage or perennial ryegrass silage.	the nutrient contents of perennial ryegrass (lolium perenne l.) and alfalfa (medicago sativa l.) are reasonably similar. despite similarities, the lactation performance of dairy cows fed perennial ryegrass has not been compared with the lactation performance of dairy cows fed alfalfa. the present study was implemented to compare the performance of lactating cows fed alfalfa or perennial ryegrass silage. alfalfa and perennial ryegrass were harvested at late bud and boot stages of maturity, respec ...	1998	9493091
endophytic fungi in indigenous australasian grasses associated with toxicity to livestock.	grazing of echinopogon spp. by livestock in australia has caused symptoms similar to those of perennial ryegrass staggers. we observed an endophytic fungus in the intercellular spaces of the leaves and seeds of new zealand and australian specimens of echinopogon ovatus. culture of surface-sterilized seeds from new zealand specimens yielded a slow-growing fungus. an examination in which immunoblotting and an enzyme-linked immunosorbent assay (elisa) were used indicated that e. ovatus plants from ...	1998	9464398
biochemical and immunological characterization of recombinant allergen lol p 1.	pollen from perennial rye grass (lolium perenne), a major cause of type-i allergy worldwide, contains a complex mixture of allergenic proteins among which lol p 1 is one of the most important. we describe the expression, purification and characterization of a recombinant lol p 1 overproduced in escherichia coli. the recombinant allergen, expressed in high yields and purified in milligram amounts, bound to specific ige antibodies from human sera, induced histamine release from sensitized human ba ...	1997	9395340
rumen fermentation and nutrient flows for cows fed grass and grass supplemented with molassed beet pulp pellets.	an experiment was carried out to determine the effect of a grass diet and a concentrate supplement on rumen fermentation and nutrient flows to the duodenum. perennial ryegrass was cut and fed indoors to eight rumen- and duodenum-cannulated friesian cows with or without 3 kg/d of molassed beet pulp in a randomized design experiment. the dry matter intake of grass was significantly lower for cows fed the concentrate supplement (13.6 vs. 11.5 kg of dry matter/d), but total dry matter and organic ma ...	1997	9361218
ige antibodies to aeroallergens in allergic children in são paulo, brazil.	the sensitization to inhaled allergens was studied in 80 brazilian children and 12 controls, aged 6 to 16 years. we performed skin tests with alternaria alternata, cat, dog, lolium perenne, grasses and the following domestic mites: blomia tropicalis, dermatophagoides pteronyssinus, dermatophagoides farinae, lepidoglyphus destructor, chortoglyphus arcutus and aleuroglyphus ovatus. the frequencies of positive skin tests (mean diameter > or = 23 mm) were respectively 15%, 11%, 11%, 6%, 7%, 95%, 92% ...	1997	9330189
intake stimulants in perennial ryegrass (lolium perenne l.) fed to sheep.	few studies exist on intake stimulants in forage plants, although a large amount of research has been conducted on deterrents to the feeding behavior of herbivore livestock. to study intake stimulants in plants, chemical components of perennial ryegrass were extracted, fractionated, and examined for their effects on the feeding behavior of sheep. methanol extracts were found to be stimulants, and pentane extracts tended to be deterrents. the ethyl acetate fraction of the methanol extracts stimul ...	1997	9313150
ozone increases group 5 allergen content of lolium perenne.		1997	9284991
infestation of sheep dung by nematophagous fungi and implications for the control of free-living stages of gastro-intestinal nematodes.	a field trial was conducted to assess the rate at which dung becomes infested by fungi which parasitise nematodes (nematophagous fungi) after deposition. sheep dung was placed on field plots of bare ground, ryegrass (lolium perenne), browntop (agrostis capillaris) and white clover (trifolium repens) in summer (february) and autumn (april), and subsamples were examined at intervals for the presence of nematophagous fungi. nematophagous fungi occurred in 71% of 129 samples recovered in february an ...	1997	9211650
viability of cryptosporidium parvum during ensilage of perennial ryegrass.	the survival of cryptosporidium parvum during ensilage of perennial ryegrass was examined in laboratory silos with herbage prepared in one of three different ways; either untreated, inoculated with a strain of lactobacillus plantarum or by direct acidification with formic acid. the ph values of all silages initially fell below 4.5, but only formic acid-treated silage remained stable at less than ph 4 after 106 d, with the ph of the untreated and inoculant-treated silages rising to above 6. the f ...	1997	9147867
immunological cross-reactivity of the major allergen from perennial ryegrass (lolium perenne), lol p i, and the cysteine proteinase, bromelain.	antibodies prepared in rabbits against the major allergen from ryegrass (lolium perenne), lol p i, cross-reacted with the cysteine proteinase bromelain from pineapple and vice versa. deglycosylation of the proteins showed that the cross-reaction was based on recognition of the carbohydrate moiety of the allergen, but for bromelain the cross-reaction was most likely due to a combination of factors. the results indicate that the carbohydrate residues from these allergens play an important role in ...	1997	9104799
grass immunotherapy induces inhibition of allergen-specific human peripheral blood mononuclear cell proliferation.	the peripheral blood mononuclear cells (pbmc) from humans allergic to grass pollens (gr+ subjects) show strong in vitro proliferative responses to purified allergens from lolium perenne pollen lol p 1, and to a lesser extent to lol p 2 and lol p 3. by contrast, pbmc from grass allergic patients undergoing immunotherapy (gr + it subjects) exhibit a very poor lol p-specific proliferative response, similar to that observed in nongrass allergic subjects (gr-subjects). unlike gr-subjects, both gr+ an ...	1997	9030100
human recombinant antibody fragments specific for a rye-grass pollen allergen: characterization and potential applications.	one of the major allergens from the pollen of perennial rye grass (lolium perenne), lol pii, was used to isolate specific antibody fragments from a random combinatorial library displaying a large repertoire of human fab on filamentous phages. after five panning cycles on recombinant lol pii immunotubes, phage binders were isolated and the antibody fragments expressed as soluble fab molecules in the escherichia coli periplasm. the dna sequencing of the clones producing antibodies with the highest ...	1996	9010244
cloning and expression pattern of hor v 9, the group 9 pollen isoallergen from barley.	in this study we report the cloning, sequence, and characterization of hor v 9 allergen cdnas from barley (hordeum vulgare) pollen. structural homologues of kentucky bluegrass (poa pratensis) group 9 pollen allergens were identified in a cdna library of barley pollen expressed mrnas. the hor v 9 cdna clone (hvp9742) contained an open reading frame encoding 313 amino acids which included a putative 27-residue signal peptide and one asparagine sequon for glycosylation. the mrna corresponding to cl ...	1996	8982067
characterization of dac g 4, a major basic allergen from dactylis glomerata pollen.	monoclonal antibodies were produced against dac g 4, a purified major basic allergen from dactylis glomerata pollen. their ability to be used for immunopurification of dac g 4 was studied on a biacore apparatus (pharmacia). the allergen was purified by affinity chromatography with one monoclonal antibody. its precise molecular mass, 59,185 +/- 30 d, was determined by mass spectrometry. its isoelectric point is 10.4. sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting show ...	1996	8977507
inhalative occupational and ingestive immediate-type allergy caused by chicory (cichorium intybus).	we report a first case of occupational allergy to chicory (cichorium intybus) in a vegetable wholesaler. symptoms occurred after oral, cutaneous or inhalatory exposure. the patient also reported reactions after ingestion of botanically related endive (cichorium endivia) and lettuce (lactuca sativa). we identified the responsible allergen by sds-page and immunoblot to be a 48-kda protein, confined to the non-illuminated parts of the plants. no cross-reactivity was found with mugwort (artemisia vu ...	1996	8877160
receiver-operating characteristic (roc) curves: a fundamental tool for improving the clinical usefulness of in vitro ige tests.	in order to establish the most efficient thresholds for serum allergen-specific ige measured by the pharmacia cap system, a "second-generation" in vitro method, we evaluated results from 89 subjects with suspected inhalant allergies, using receiver-operating characteristic (roc) curve analysis. sera samples were analyzed by the pharmacia cap system for specific ige, the choice of allergens to be tested being based upon the symptoms and clinical history of each patient. results were analyzed by r ...	1996	8837664