Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| fungal plant pathogens in portugal: alternaria dauci. | a leaf blight of carrot (daucus carota) caused by alternaria dauci was found in alentejo (ourique, portugal). morphological characteristics of the fungus are described. | 2008 | 19071897 |
| dna analysis of a radiotolerant bacterium pantoea agglomerans by ft-ir spectroscopy. | a radiation tolerance strain, pantoea agglomerans was isolated from gamma-irradiated carrot samples (daucus carota). d(10) determination showed that the radioresistance of this bacterium is five-fold higher than escherichia coli, both belonging to the family of enterobacteriaceae. dna isolated from untreated and irradiated bacterial cells was analyzed by ft-ir spectroscopy to investigate the radiotolerance of this bacterium. at doses <5kgy, an alteration of the interbase hydrogen networks was ob ... | 2007 | 19071550 |
| sulfur transfer through an arbuscular mycorrhiza. | despite the importance of sulfur (s) for plant nutrition, the role of the arbuscular mycorrhizal (am) symbiosis in s uptake has received little attention. to address this issue, 35s-labeling experiments were performed on mycorrhizas of transformed carrot (daucus carota) roots and glomus intraradices grown monoxenically on bicompartmental petri dishes. the uptake and transfer of 35so4(2-) by the fungus and resulting 35s partitioning into different metabolic pools in the host roots was analyzed wh ... | 2009 | 18978070 |
| chemical characterization and biological activity of essential oils from daucus carota l. subsp. carota growing wild on the mediterranean coast and on the atlantic coast. | the essential oils and supercritical co(2) extracts of wild daucus carota l. subsp. carota growing spontaneously in sardinia and in portugal were investigated. the main components in the sardinian essential oil of flowering and mature umbels with seeds are beta-bisabolene (17.6-51.0%) and 11-alpha-(h)-himachal-4-en-1-beta-ol (9.0-21.6%); instead, the oils from portuguese samples are predominantly composed of geranyl acetate (5.2-65.0%) and alpha-pinene (3.5-37.9%). supercritical extracts contain ... | 2009 | 18950693 |
| a small rna resembling the beet western yellows luteovirus st9-associated rna is a component of the california carrot motley dwarf complex. | abstract virions were purified from anthriscus cerefolium or coriandrum sativum plants infected with the viruses that cause california carrot motley dwarf. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of virion preparations yielded a single prominent protein species of approximately 28,000 molecular weight; however, denaturing agarose gel electrophoresis showed that virions contained three prominent single-stranded rnas of approximately 5.6, 4.2, and 2.8 kb. northern hybridization a ... | 1998 | 18944986 |
| long-term biosanitation by application of coniothyrium minitans on sclerotinia sclerotiorum-infected crops. | abstract the effect of the fungal mycoparasite coniothyrium minitans applied as a spray to crops infected with sclerotinia sclerotiorum (causal agent of white mold) on contamination of soil with s. sclerotiorum sclerotia was studied in a 5-year field experiment. sclerotial survival also was monitored during two subsequent years, when the field was returned to commercial agriculture. in a randomized block design, factorial combinations of four crops and three treatments were repeated 10 times. po ... | 1999 | 18944788 |
| use of heteroduplex mobility assay for identification and differentiation of phytoplasmas in the aster yellows group and the clover proliferation group. | abstract this paper describes the identification and differentiation of phytoplasmas by a highly sensitive diagnostic technique, dna heteroduplex mobility assay (hma). closely related phytoplasma isolates of clover proliferation (cp), potato witches'-broom (pwb), and alfalfa witches'-broom (awb) were collected from the field from 1990 to 1999. the entire 16s rrna gene and 16/23s spacer region were amplified by polymerase chain reaction (pcr) from the field samples and standard cp, pwb, and awb p ... | 2001 | 18943942 |
| reinvestigation of the bitter compounds in carrots (daucus carota l.) by using a molecular sensory science approach. | in order to reinvestigate the key molecules inducing bitter off-taste of carrots ( daucus carota l.), a sensory-guided fractionation approach was applied to bitter carrot extracts. besides the previously reported bitter compounds, 6-methoxymellein (1), falcarindiol (2), falcarinol (3), and falcarindiol-3-acetate (4), the following compounds were identified for the first time as bitter compounds in carrots with low bitter recognition thresholds between 8 and 47 micromol/l: vaginatin (5), isovagin ... | 2008 | 18922011 |
| lowering of intraocular pressure by topical application of daucus carota seed extract in rabbits. | in normotensive rabbits topical application of daucus carota seed extract at the concentration of 0.3, 0.6 and 1.2% resulted in mean iop reduction of 19.33. 23.20 and 25.61% respectively from baseline. as no significant difference was observed between the change in iop in 0.6 and 1.2% extract treated groups, 0.6% concentration was chosen for further evaluation in rabbits with experimentally elevated iop. in water loaded rabbits, maximum mean iop reduction with 0.6% extract was 29.39%, which was ... | 2008 | 18807759 |
| isolation of development-related genes in somatic embryo radicle of carrot (daucus carota l.) using modified cdna rda. | using modified cdna rda capitalizing on the high affinity of streptavidin for biotin and magnetic-absorption-based separation, we have obtained four bands of specifically expressed cdna in the carrot somatic embryo deregulated for 12 h, which were designated as nr-1, nr-2, nr-3 and nr-4, respectively. as revealed by homology analysis of their dna sequences after cloning them into pbs, remarkable homology was demonstrated in nr-2, nr-3 and nr-4 with the genes coding for lea (late embryogenesis ab ... | 2000 | 18763115 |
| partial nucleotide sequence of a carlavirus from carrot (daucus carota l.). | 2008 | 18751883 | |
| structural responses of daucus carota root-organ cultures and the arbuscular mycorrhizal fungus, glomus intraradices, to 12 pharmaceuticals. | pharmaceuticals and personal care products may enter the terrestrial environment through the amendment of agricultural soils with manure or biosolids with potential impacts on beneficial soil microbe populations. the beneficial symbiotic relationship between most plant species and arbuscular mycorrhizal fungi is a primary determinant of plant health and soil fertility. as such, there is increasing recognition of the need to study the impacts of anthropogenic stressors on plant-microbe interactio ... | 2008 | 18656230 |
| functional characteristics of a tiny but specialized olfactory system: olfactory receptor neurons of carrot psyllids (homoptera: triozidae). | with only approximately 50 olfactory receptor neurons (orns), the carrot psyllid trioza apicalis (homoptera: psylloidea) may have the smallest olfactory system described in adult neopteran insects. using single sensillum recordings (ssr) and gas chromatograph-linked ssr, we characterized 4 olfactory sensilla forming a distinct morphological type, which together house approximately 25% of all orns. we recorded responses to extracts and single constituents from daucus carota ssp. sativus, from the ... | 2008 | 18653644 |
| expression and assembly of cholera toxin b subunit (ctb) in transgenic carrot (daucus carota l.). | we expressed the cholera toxin b subunit (ctb) fused to an endoplasmic reticulum retention signal (sekdel) in carrot roots using an agrobacterium-mediated transformation method. fourteen independent transgenic lines were regenerated via somatic embryogenesis after 6 months of culture. the sctb gene was detected in the genomic dna of transgenic carrot by pcr amplification. expressions and assembly of sctb protein into oligomeric structures of pentameric size were observed in transgenic plant extr ... | 2009 | 18642102 |
| population and biomass kinetics in fed-batch cultures of daucus carota l. somatic embryos. | the population dynamics of developing somatic embryos of carrot (daucur carota l.) was investigated in batch and fed-batch cultures using modified murashige and skoog medium. these substrate limitations coincided not only with stoppage of biomass increase, but also with the increase in total concentration of embryos as well as the advancement of the embryo into a more mature stage. both glucose and ammonium were depleted from the culture. restoring either glucose, or ammonium and nitrate, as to ... | 1993 | 18609625 |
| essential oil of daucus carota subsp. halophilus: composition, antifungal activity and cytotoxicity. | essential oils are known to possess antimicrobial activity against a wide spectrum of bacteria and fungi. daucus carota l. is used since olden times in traditional medicine, due to recognized therapeutic properties, namely the antimicrobial activity of their essential oils. | 2008 | 18606215 |
| upregulation of the promoter activity of the carrot (daucus carota) phenylalanine ammonia-lyase gene (dcpal3) is caused by new members of the transcriptional regulatory proteins, dcerf1 and dcerf2, which bind to the gcc-box homolog and act as an activator to the dcpal3 promoter. | the phenylalanine ammonia-lyase (pal) gene, dcpal3, was expressed during the synthesis of anthocyanin in suspension-cultured cells of carrot (daucus carota). there were two putative cis-elements in the dcpal3 promoter region: the box-l and gcc-box homologs. both of these are committed to the upregulation of promoter activity. although box-l is known as the conserved cis-element present in the promoter region of most pal genes of many plant species targeted by the r2r3-myb protein, among pal gene ... | 2008 | 18584290 |
| effect of decontamination agents on the microbial population, sensorial quality, and nutrient content of grated carrots (daucus carota l.). | several decontamination agents including water, sodium hypochlorite, peroxyacetic acid, neutral electrolyzed oxidizing water, and chlorine dioxide gas were tested for their effectiveness to reduce the natural microflora on grated carrots. microbial reductions of the total aerobic count obtained after the different treatments varied between 0.11 and 3.29 log colony-forming units (cfu)/g. whether or not a decontamination step induced significant changes in the sensory attributes of grated carrots ... | 2008 | 18582083 |
| a semicontinuous culture model that links cell growth to extracellular nutrient concentration. | during semicontinuous culture, a sample of fixed volume is removed at regular time intervals to make measurements and/or harvest culture components, and an equal volume of fresh medium is immediately added to the culture, thereby instantaneously enhancing nutrient concentrations and diluting cell concentration. the resulting cell concentration versus time curve (i.e., the actual cell growth curve) has a saw-toothed appearance because of the periodic dilution of cell concentration. the observed c ... | 1986 | 18553861 |
| asymmetric reduction of prochiral ketones to chiral alcohols catalyzed by plants tissue. | as an important organic compound, chiral alcohols are the key chiral building blocks to many single enantiomer pharmaceuticals. asymmetric reduction of the corresponding prochiral ketones to produce the chiral alcohols by biocatalysis is one of the most promising routes. asymmetric reduction of different kinds of non-natural prochiral ketones catalyzed by various plants tissue was studied in this work. acetophenone, 4'-chloroacetophenone and ethyl 4-chloroacetoacetate were chosen as the model su ... | 2008 | 18548304 |
| diversity of dcmaster-like elements of the pif/harbinger superfamily in the carrot genome. | transposable elements constitute a significant fraction of plant genomes. both autonomous and non-autonomous elements of the dcmaster family, residing in the carrot genome, were described previously. dcmaster elements were classified as members of the pif/harbinger superfamily. in the present paper we report on the identification of other groups of dcmaster-like elements. beside the previously described, relatively highly abundant miniature inverted repeat element (mite) family krak (ca. 3,600 c ... | 2009 | 18535910 |
| prepenetration apparatus assembly precedes and predicts the colonization patterns of arbuscular mycorrhizal fungi within the root cortex of both medicago truncatula and daucus carota. | arbuscular mycorrhizas (am) are widespread, ancient endosymbiotic associations that contribute significantly to soil nutrient uptake in plants. we have previously shown that initial fungal penetration of the host root is mediated via a specialized cytoplasmic assembly called the prepenetration apparatus (ppa), which directs am hyphae through the epidermis (genre et al., 2005). in vivo confocal microscopy studies performed on medicago truncatula and daucus carota, host plants with different patte ... | 2008 | 18515499 |
| novel mechanism of modulating natural antioxidants in functional foods: involvement of plant growth promoting rhizobacteria nrrl b-30488. | the significance of plant growth-promoting rhizobacteria (pgpr) mediated increase in antioxidant potential in vegetables is yet unknown. the plant growth-promoting bacterium bacillus lentimorbus nrrl b-30488 (b-30488) mediated induction of dietary antioxidant in vegetables ( trigonella foenum-graecum, lactuca sativa, spinacia oleracea, and daucus carota) and fruit ( citrus sinensis) after minimal processing (fresh, boiled, and frozen) was tested by estimating the total phenol content, level of a ... | 2008 | 18491912 |
| color evolution of aqueous solutions obtained by thermal processing of carrot (daucus carota l.) roots: influence of light. | the color of aqueous solutions obtained by heating carrot (daucus carota l.) roots in water ("stocks") is different when the thermal treatment is applied with or without exposure to light. cie l*, a*, and b* scale values of stocks processed for different times were recorded and 4 patterns were initially observed. to explain the 1st part of this evolution (patterns 1 and 2), pectin extraction and beta-elimination in stocks were studied. light dependence was investigated to explain patterns 3 and ... | 2008 | 18460127 |
| spatial and temporal expression profiling of cell-wall invertase genes during early development in hybrid poplar. | cell-wall invertase genes are spatially and temporally regulated in several plant species, including daucus carota l., lycopersicon esculentum l. and solanum tuberosum l. however, few studies of cell-wall invertase genes of trees have been conducted, despite the importance of trees as a source of lignocellulosic biopolymers. we identified three putative cell-wall invertase genes in hybrid poplar (populus alba l. x grandidentata michx.) that showed higher homology to each other than to cell-wall ... | 2008 | 18450570 |
| ionic thiocyanate (scn(-)) production, fate, and phytotoxicity in soil amended with brassicaceae seed meals. | brassicaceae seed meals produce ionic thiocyanate (scn (-)), a bioherbicidal compound. this study determined the fate of scn (-) in a field soil amended with seed meals of sinapis alba, brassica juncea, and brassica napus and quantified crop phytotoxicity by monitoring carrot ( daucus carota) emergence. meals were applied at 1 or 2 t ha (-1), and soils were sampled to 35 cm for scn (-). maximum scn (-) (211 micromol kg (-1) of soil) was measured at 5 days in 0-5 cm samples from plots amended wit ... | 2008 | 18442242 |
| purple carrot (daucus carota l.) polyacetylenes decrease lipopolysaccharide-induced expression of inflammatory proteins in macrophage and endothelial cells. | carrots ( daucus carota l.) contain phytochemicals including carotenoids, phenolics, polyacetylenes, isocoumarins, and sesquiterpenes. purple carrots also contain anthocyanins. the anti-inflammatory activity of extracts and phytochemicals from purple carrots was investigated by determining attenuation of the response to lipopolysaccharide (lps). a bioactive chromatographic fraction (sephadex lh-20) reduced lps inflammatory response. there was a dose-dependent reduction in nitric oxide production ... | 2008 | 18433135 |
| a carrot g-box binding factor-type basic region/leucine zipper factor dcbz1 is involved in abscisic acid signal transduction in somatic embryogenesis. | carrot (daucus carota) somatic embryogenesis has been extensively used as an experimental system for studying embryogenesis. in maturing zygotic embryos, abscisic acid (aba) is involved in acquisition of desiccation tolerance and dormancy. on the other hand, somatic embryos contain low levels of endogenous aba and show desiccation intolerance and lack dormancy, but tolerance and dormancy can be induced by exogenous application of aba. in aba-treated carrot embryos, some aba-inducible genes are e ... | 2016 | 18407508 |
| cadmium accumulation in the edible parts of different cultivars of radish, raphanus sativus l., and carrot, daucus carota var. sativa, grown in a cd-contaminated soil. | cadmium accumulation among 12 cultivars of radish (raphanus sativus) and 10 cultivars of carrot (daucus carota var. sativa) was studied in a cd-contaminated soil. the cd concentration in the edible parts of radish and carrot ranged from 0.04 to 0.14 and 0.14 to 0.19 mg kg(-1) fresh weight, respectively. all the tested carrot cultivars and 33% of the tested radish cultivars exceeded the chinese allowable limit for cd. the study showed a greater scope for selecting radish cultivars than for carrot ... | 2008 | 18392549 |
| epr as an analytical tool in assessing the mineral nutrients and irradiated food products-vegetables. | epr spectral investigations of some commonly available vegetables in south india, which are of global importance like daucus carota (carrot), cyamopsis tetragonoloba (cluster beans), coccinia indica (little gourd) and beta vulgaris (beet root) have been carried out. in all the vegetable samples a free radical corresponding to cellulose radical is observed. almost all the samples under investigation exhibit mn ions in different oxidation states. the temperature variation epr studies are done and ... | 2008 | 18372211 |
| survival and flowering of hybrids between cultivated and wild carrots (daucus carota) in danish grasslands. | many crop species are able to hybridize with related weedy or wild relatives, which could lead to transfer of cultivar genes, and among them transgenes, into wild populations. it is not clear, however, whether the hybrids and their descendants are able to survive and reproduce in natural habitats, as inherited cultivar traits may be maladaptive under such conditions. to test this, we produced hybrid (f(1)) seeds by controlled crosses between wild [see text for formula] and cultivated carrots (da ... | 2008 | 18289499 |
| dcmaster transposon display markers as a tool for diversity evaluation of carrot breeding materials and for hybrid seed purity testing. | in recent years, transposon insertion polymorphisms have been utilized as molecular markers, and a range of techniques tailored towards identification of insertion sites of various transposable elements have been developed. in the present paper we describe the application of a recently developed dcmaster transposon display system to analyse the genetic diversity of polish breeding materials of carrot (daucus carota) and to identify polymorphisms useful for hybrid seed purity testing. using 3 set ... | 2008 | 18263967 |
| mitochondrial regulation of flower development. | flower development in plants depends not only on a set of nuclear genes but also on the coordinate action of the mitochondrion. certain mitochondrial genomes in combination with certain nuclear genomes lead to the expression of cytoplasmic male-sterility (cms). both mitochondrial genes that determine male-sterility and nuclear restorer-of-fertility genes that suppress the male-sterile phenotype have been cloned. lately, the interactions between mitochondrial and nuclear genes through retrograde ... | 2008 | 18054525 |
| mitochondrial nad2 gene is co-transcripted with cms-associated orfb gene in cytoplasmic male-sterile stem mustard (brassica juncea). | the transcriptional patterns of mitochondrial respiratory related genes were investigated in cytoplasmic male-sterile and fertile maintainer lines of stem mustard, brassica juncea. there were numerous differences in nad2 (subunit 2 of nadh dehydrogenase) between stem mustard cms and its maintainer line. one novel open reading frame, hereafter named orfb gene, was located at the downstream of mitochondrial nad2 gene in the cms. the novel orfb gene had high similarity with ymf19 family protein, or ... | 2009 | 18046626 |
| determination of kinetic and equilibrium parameters of the batch adsorption of mn(ii), co(ii), ni(ii) and cu(ii) from aqueous solution by black carrot (daucus carota l.) residues. | in this study, the effect of temperature on the adsorption of mn(ii), ni(ii), co(ii) and cu(ii) from aqueous solution by modified carrot residues (mcr) was investigated. the equilibrium contact times of adsorption process for each heavy metals-mcr systems were determined. kinetic data obtained for each heavy metal by mcr at different temperatures were applied to the lagergren equation, and adsorption rate constants (kads) at these temperatures were determined. these rate constants related to the ... | 2008 | 17980960 |
| selecting and evaluating native plants for region-specific phytotoxicity testing. | in this study, we evaluated methodology to determine risks to terrestrial native plant species from potential herbecide drift, focusing on 1) selection of native species for testing, 2) growth of these species, and 3) variability in herbicide response among native species and compared with crop plants. native plant species were selected for initial testing on the basis of spatial analysis, which indicated that species from illinois, usa, were at potential risk for off-target effects of herbicide ... | 2008 | 17973566 |
| improvement of texture properties and flavor of frozen dough by carrot (daucus carota) antifreeze protein supplementation. | the effects of concentrated carrot protein (ccp) containing 15.4% (w/w) carrot (daucus carota) antifreeze protein on texture properties of frozen dough and volatile compounds of crumb were studied. ccp supplementation lowered the freezable water content of the dough, resulting in some beneficial effects including holding loaf volume steadily and making the dough softer and steadier during frozen storage. furthermore, spme-gc-ms analysis showed ccp supplementation did not give any negative influe ... | 2007 | 17935294 |
| simultaneous modelling of the thermal degradation kinetics of pectin methylesterase in lettuce (lactuca sativa l.) and carrot (daucus carota l.) extracts: analysis of seasonal variation and tissue type. | the thermal degradation kinetics of pectin methylesterase (pme) from carrot and lettuce were studied. fresh extracts were exposed to temperatures from 55 to 70 degrees c until the enzyme was inactivated. a model based on the presence of two forms of the enzyme, one active and one non-active, is proposed. the natural variability of the pme activity was taken into the model in the form of normally distributed random effects. the common model parameters obtained (cleavage constant (0.0395+/-0.0062 ... | 2007 | 17928712 |
| comparative expression of beta-glucuronidase with five different promoters in transgenic carrot (daucus carota l.) root and leaf tissues. | tissue-specific patterns and levels of protein expression were characterized in transgenic carrot plants transformed with the beta-glucuronidase (gus) gene driven by one of five promoters: cauliflower mosaic virus 35s (35s) and double 35s (d35s), arabidopsis ubiquitin (ubq3), mannopine synthase (mas2) from agrobacterium tumefaciens or the rooting loci promoter (rold) from a. rhizogenes. five independently transformed carrot lines of each promoter construct were assessed for gus activity. in leav ... | 2008 | 17924115 |
| the zinc binding site of the shaker channel kdc1 from daucus carota. | kdc1 is a voltage-dependent shaker-like potassium channel subunit cloned from daucus carota which produces conductive channels in xenopus oocytes only when coexpressed with other plant shaker potassium subunits, such as kat1 from arabidopsis thaliana. external zn(2+) determines a potentiation of the current mediated by the dimeric construct kdc1-kat1, which has been ascribed to zinc binding at a site comprising three histidines located at the s3-s4 (h161, h162) and s5-s6 (h224) linkers of kdc1. ... | 2008 | 17890387 |
| little nuclei genes affecting nuclear morphology in arabidopsis thaliana. | efforts to understand nuclear organization in plant cells have received little assistance from the better-studied animal nuclei, because plant proteomes do not contain recognizable counterparts to the key animal proteins involved in nuclear organization, such as lamin nuclear intermediate filament proteins. previous studies identified a plant-specific insoluble nuclear protein in carrot (daucus carota), called nuclear matrix constituent protein1 (nmcp1), which contains extensive coiled-coil doma ... | 2007 | 17873096 |
| content and in-vitro accessibility of pro-vitamin a carotenoids from sri lankan cooked non-leafy vegetables and their estimated contribution to vitamin a requirement. | vitamin a deficiency is a public health problem in sri lanka, which affects especially pre-school children. carrots (daucus carota), pumpkins (cucurbita maxima), squashes (cucurbita moschata) and sweet potatoes (ipomoea batatas) of orange, yellow-fleshed varieties are good sources of provitamin a carotenoids, but have not been studied in sri lanka in terms of the food as eaten. the content of carotenoids in each preparation method and the in-vitro accessibility of beta-carotene and alpha-caroten ... | 2007 | 17852483 |
| morphogenetic responses of cultured totipotent cells of carrot (daucus carota var. carota) at zero gravity. | an experiment designed to test whether embryos capable of developing from isolated somatic carrot cells could do so under conditions of weightlessness in space was performed aboard the unmanned soviet biosatellite kosmos 782 under the auspices of the joint united states-soviet biological satellite mission. space flight and weightlessness seem to have had no adverse effects on the induction of embryoids or on the development of their organs. a portion of the crop of carrot plantlets originated in ... | 1978 | 17847331 |
| cloning and expression of dcga gene encoding alpha subunit of gtp-binding protein in carrot seedlings. | a homology-based cloning strategy yielded a cdna clone designated dcga, presumably encoding alpha subunit of gtp-binding protein, from carrot (daucus carota) seedlings. molecular phylogenetic tree analysis of g protein alpha subunits from various biological sources suggested that, unlike in animal cells, classification of galpha proteins into specific subfamilies could not be applicable to the proteins from higher plants. the restriction digests prepared from genomic dna of carrot showed one or ... | 2007 | 17827744 |
| transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. | the luciferase gene from the firefly, photinus pyralis, was used as a reporter of gene expression by light production in transfected plant cells and transgenic plants. a complementary dna clone of the firefly luciferase gene under the control of a plant virus promoter (cauliflower mosaic virus 35s rna promoter) was introduced into plant protoplast cells (daucus carota) by electroporation and into plants (nicotiana tabacum) by use of the agrobacterium tumefaciens tumor-inducing plasmid. extracts ... | 1986 | 17758108 |
| arginine decarboxylase and polyamines required for embryogenesis in the wild carrot. | embryogenic cultures of daucus carota treated with 1 millimolar alpha-difluoromethylarginine, a specific inhibitor of arginine decarboxylase, exhibited nearly a 50 percent reduction in embryo formation compared with controls. putrescine and spermidine concentrations in the treated cells were greatly reduced. addition of putrescine, spermidine, or spermine to the culture medium restored embryogenesis in the treated cultures. embryogenesis was not significantly affected by alpha-difluoromethylorni ... | 1984 | 17746056 |
| enantioselective hydrolysis of bromo- and methoxy-substituted 1-phenylethanol acetates using carrot and celeriac enzymatic systems. | enantioselective hydrolysis of bromo- and methoxy-substituted 1-phenylethanol acetates was conducted using comminuted carrot (daucus carota l.) and celeriac (apium graveolens l. var. rapaceum) roots. hydrolysis of the acetates led to alcohols, preferentially to r-(+)-enantiomers. efficiencies of both reactions - hydrolysis of the acetates with an electron-donating methoxy group and oxidation of the resulting alcohols - increased in the following order: ortho < meta < para. the presence of an ele ... | 2013 | 17708446 |
| (e)-methylisoeugenol and elemicin: antibacterial components of daucus carota l. essential oil against campylobacter jejuni. | the essential oil of wild daucus carota l. obtained from aerial parts at the end of the flowering stage (dceo) was reported as antimicrobial against the human enteropathogen campylobacter jejuni. the aim of the present study was to extend this analysis to other campylobacter species and to identify the active compounds of the essential oil, subjected to gc, gc-ms, and (13)c nmr analysis. a minimum inhibitory concentration assay was used to quantify the antimicrobial activity of dceo and the majo ... | 2007 | 17685629 |
| metabolic engineering of novel ketocarotenoid production in carrot plants. | carotenoids constitute a vast group of pigments that are ubiquitous throughout nature. carrot (daucus carota l.) roots provide an important source of dietary beta-carotene (provitamin a), alpha-carotene and lutein. ketocarotenoids, such as canthaxanthin and astaxanthin, are produced by some algae and cyanobacteria but are rare in plants. ketocarotenoids are strong antioxidants that are chemically synthesized and used as dietary supplements and pigments in the aquaculture and neutraceutical indus ... | 2008 | 17682834 |
| evidence for p-hydroxybenzoate formation involving enzymatic phenylpropanoid side-chain cleavage in hairy roots of daucus carota. | in this study, methyl jasmonate (mj)-elicited hairy root cultures of daucus carota were explored to study the enzymatic route to p-hydroxybenzoic acid (p-hba) biosynthesis. treatment with 100microm mj caused an enhanced accumulation of p-hba as well as total phenolic content in elicited root lines as compared to untreated (controls) lines. using cell-free extract as the source of crude enzymes, attempt was made to reveal the enzymatic route to p-hba formation. the accumulation of p-hba was prece ... | 2008 | 17658659 |
| [advanced study on serk genes family]. | somatic embryogenesis receptor-like kinase (serk) genes were identified in different plant species, such as daucus carota, arabidopsis thaliana and oryza sativa. studies of these genes showed that they have conserved structures and expression patterns. these genes are not only expressed in embryogenic tissues, but also in post-embryogenic development. they function in embryogenesis and reproductive development and defences in particular, related signal transduction pathways. | 2007 | 17650484 |
| differential effects of hexaconazole and paclobutrazol on biomass, electrolyte leakage, lipid peroxidation and antioxidant potential of daucus carota l. | the application of triazole fungicides is a common practice in the cultivation of carrot (daucus carota l.) plants. it is there for seems important to test the changes that are occurring in this food crop under triazoles, the non-traditional plant growth regulators, treatments in order to identify the extent to which it tolerate the fungicide application and thereby make it an economical food crop. a field experiment was conducted to find out the effects of two triazole fungicides (hexaconazole ... | 2007 | 17644352 |
| biostimulation of pah degradation with plants containing high concentrations of linoleic acid. | many plant species enhance the biodegradation of polycyclic aromatic hydrocarbons (pahs), but there is little understanding of the mechanisms by which this occurs. this research identified phytochemicals that stimulate pyrene degradation using crushed roottissues from 43 plants that were screened in soil spiked with 100 ppm pyrene. among the plants tested, root tissues from apium graveolens (celery), raphanus sativus (radish), solanum tuberosum (potato), and daucus carota (carrot) were most effe ... | 2007 | 17626440 |
| a predictive nutritional model for plant cells and hairy roots. | a structured nutritional model is proposed to describe growth and nutritional behavior of eschscholtzia californica suspension cells and catharanthus roseus and daucus carota hairy roots in in vitro culture. the model describes the cells specific growth rate from concentration of intracellular nutrients such as inorganic phosphate (pi), nitrogen sources (no(3) (-) and nh(4) (+)) and sugars. two-level michaelis-menten kinetics are used to describe pi and no(3) (-) uptake and simple michaelis-ment ... | 2008 | 17614323 |
| identification and characterization of a new virus in the genus potyvirus from wild populations of angelica lucida l. and a. genuflexa nutt., family apiaceae. | a novel potyvirus was discovered in angelica lucida l. (wild celery) and a. genuflexa nutt. (kneeling angelica) (family apiaceae) in the matanuska- susitna valley, alaska. the experimental plant host range of the virus included species in three families: chenopodiaceae (chenopodium amaranticolor coste et reyn and chenopodium quinoa willd.), solanaceae (nicotiana benthamiana domin, and n. clevlandii gray.), and apiaceae (anethum graveolens l., apium graveolens l. var. dulce (miller)), daucus caro ... | 2007 | 17558540 |
| results of a screening programme to identify plants or plant extracts that inhibit ruminal protein degradation. | one aim of the ec framework v project, 'rumen-up' (qlk5-ct-2001-00 992), was to find plants or plant extracts that would inhibit the nutritionally wasteful degradation of protein in the rumen. a total of 500 samples were screened in vitro using 14c-labelled casein in a 30-min incubation with ruminal digesta. eight were selected for further investigation using a batch fermentation system and soya protein and bovine serum albumin as proteolysis substrates; proteolysis was monitored over 12 h by th ... | 2007 | 17445338 |
| determination of polyacetylenes in carrot roots (daucus carota l.) by high-performance liquid chromatography coupled with diode array detection. | a new high-performance liquid chromatographic method with diode array detection was developed for the separation and simultaneous determination of the carrot polyacetylenes falcarindiol (fadoh), falcarindiol 3-acetate (fadoac) and falcarinol (faoh) in carrot root extracts. the optimal chromatographic conditions were achieved on a c18 column with a linear gradient elution of water and acetonitrile as mobile phases, at the flow rate of 1.0 ml/min. all calibration curves of the three carrot polyace ... | 2007 | 17444217 |
| simulation of germination of pioneer species along an experimental drought gradient. | the germination of ten plant species from the iberian peninsula was assessed along a water deficit gradient between -0. 1652 (moist) and -0.4988 mpa (dry) of osmotic potential, created by addition of increasing concentrations of polyethylene glycol (peg 6000) to distilled water in which plants were grown hydroponically. the level and rate of germination of daucus carota and thapsia villosa significantly decreased with decreasing psi. seeds of dactylis glomerata and dittrichia viscosa had positiv ... | 2006 | 17405330 |
| biotransformation of cardenolides by plant cell cultures1. | a culture of daucus carota cell line ca68, previously shown to specifically hydroxylate digitoxigenin at the c-5 position, has been shown also to convert gitoxigenin and oleandrigenin to their corresponding 5beta-hydroxy derivatives. these two products have not been identified previously. 5beta-hydroxygitoxigenin has been identified by physico-chemical methods and data on its mass spectrum and (13)c-nmr spectrum are reported. 5beta-hydroxyoleandrigenin was identified from its high resolution mas ... | 1981 | 17401955 |
| a new flavone glycoside from seeds of daucus carota. | phytochemical examination of seeds of daucus carota l. resulted in the isolation of apigenin -4'-o-beta-d-glucoside, kaempferol-3-o-beta-d-glucoside and a new flavone glycoside which was characterised as apigenin-7-o-beta-d-galactopyranosyl-(1-->4)-o-beta-d-mannopyranoside. | 1982 | 17396982 |
| carrot infection by alternaria radicina in field conditions and results of laboratory tests. | carrot black rot caused by the fungus alternaria radicina (meier) drechsler et eddy, the economically important disease of carrot (daucus carota l.). research was carried out in 2004 and 2005 in order to establish correlation between plant infestation in the field and laboratory experiments. to determine the incidence and severity of the disease in mature crop, a 5-degree scale was used, where 1 meant no visible disease symptoms and 5 meant total foliage infestation. plants were rated individual ... | 2006 | 17390868 |
| [photosynthetic characteristics and coenological survey of lactuca serriola in its invaded area]. | lactuca serriola, a national class quarantine object, is a new invasive species in the coastal area of southeast china. the coenological survey showed that because of its big individual, l. serriola could easily form dominant population in its invaded area, and its main accompany species were conyza canadensis, c. bonarinisis, bidentis bipinnata, oenothera laciniata, ipomoea hederacea, setaria viridis, daucus carota, xanthium sibiricum, erigeron annuus, l. indica, humulus scandens, solanum nigru ... | 2006 | 17330472 |
| expression of escherichia coli heat-labile enterotoxin b subunit (ltb) in carrot (daucus carota l.). | we expressed the b subunit of enterotoxigenic escherichia coli heat-labile enterotoxin (ltb) encoded by a synthetic codon-optimized gene in carrot. an agrobacterium-mediated transformation method was used. thirty independent transgenic lines were regenerated via somatic embryogenesis after 6 months in culture and were transferred to a greenhouse. gm1-elisa assay was used to assess ltb protein content in mature taproots. some transgenic lines expressed ltb up to 0.3% of the total soluble protein, ... | 2007 | 17310334 |
| shelf-life extension of minimally processed carrots by gaseous chlorine dioxide. | chlorine dioxide (clo(2)) gas is a strong oxidizing and sanitizing agent that has a broad and high biocidal effectiveness and big penetration ability; its efficacy to prolong the shelf-life of a minimally processed (mp) vegetable, grated carrots (daucus carota l.), was tested in this study. carrots were sorted, their ends removed, hand peeled, cut, washed, spin dried and separated in 2 portions, one to be treated with clo(2) gas and the other to remain untreated for comparisons. mp carrots were ... | 2007 | 17306398 |
| the impact of cu treatment on phenolic and polyamine levels in plant material regenerated from embryos obtained in anther culture of carrot. | the influence of copper sulphate on the regeneration of carrot (daucus carota l.) androgenic embryos and changes in the levels of phenolic substances and polyamines that might be indicative of the response to oxidative stress were investigated. the cultivation on the regeneration medium supplemented with cu(2+) at the concentrations 1 and 10 microm for 15 weeks resulted in significant dose-dependent inhibition of the growth and organogenic ability of carrot embryos. the total content of phenolic ... | 2007 | 17303431 |
| carotenoid biosynthesis structural genes in carrot (daucus carota): isolation, sequence-characterization, single nucleotide polymorphism (snp) markers and genome mapping. | carotenoid pigments are important components of the human diet and carrots are the main dietary sources of the vitamin a precursors alpha- and beta-carotene. carotenoids play essential biological roles in plants and the genes coding for the carotenoid pathway enzymes are evolutionarily conserved, but little information exists about these genes for carrot. in this study, we utilized published carrot sequences as well as heterologous pcr approaches with primers derived from sequence information of ... | 2007 | 17186217 |
| enzymatic evidence for the key role of arginine in nitrogen translocation by arbuscular mycorrhizal fungi. | key enzymes of the urea cycle and (15)n-labeling patterns of arginine (arg) were measured to elucidate the involvement of arg in nitrogen translocation by arbuscular mycorrhizal (am) fungi. mycorrhiza was established between transformed carrot (daucus carota) roots and glomus intraradices in two-compartment petri dishes and three ammonium levels were supplied to the compartment containing the extraradical mycelium (erm), but no roots. time courses of specific enzyme activity were obtained for gl ... | 2007 | 17142485 |
| carrot antifreeze protein does not exhibit the polygalacturonase-inhibiting activity of pgip family. | the carrot (daucus carota) antifreeze protein (dcafp) has a strong antifreeze activity and identified as belonging to the plant polygalacturonase-inhibiting protein (pgip) family based on its sequence similarities, including the presence of a leucine-rich repeat (lrr) motif. in this study, yeast two-hybrid technology was used to analyze whether the carrot afp could act as a pgip. the complete dcafp and polygalacturonase (pgase; obtained from fungus alternaria alternata by rt-pcr) coding sequence ... | 2006 | 17112975 |
| introduction of the carrot hsp17.7 into potato (solanum tuberosum l.) enhances cellular membrane stability and tuberization in vitro. | we have examined the ability of a carrot (daucus carota l.) heat shock protein gene encoding hsp17.7 (dchsp17.7) to confer enhanced heat tolerance to potato (solanum tuberosum l.), a cool-season crop. the dchsp17.7 gene was fused to a 6xhistidine (his) tag to distinguish the engineered protein from endogenous potato proteins and was introduced into the potato cultivar 'désirée' under the control of the cauliflower mosaic virus (camv) 35s promoter. western analysis showed that engineered dchsp17. ... | 2006 | 17086756 |
| efficacy of eight larvicidal botanical extracts from khaya senegalensis and daucus carota against culex annulirostris. | the failure to discover a significant new class of insecticides has led many researchers back to biodiscovery studies in the search for new and economically viable alternatives. after a preliminary screening of botanical extracts using descending series of concentrations (1,000, 500, 100, 50, and 5 mg/liter), 8 extracts from 2 potential botanical agents, khaya senegalensis (desrousseaux) and daucus carota l., were tested against 4th instars of culex annulirostris (skuse) following the standard w ... | 2006 | 17067042 |
| carrot (daucus carota l.). | plants are susceptible to infection by a broad range of fungal pathogens. many horticulturally important crop species lack adequate genetic resistance to disease. studies on potential mechanisms of disease resistance in plants have revealed the importance of a range of pathogenesis-related (pr) proteins with antifungal activity in reducing colonization of plant tissues by pathogens. we are evaluating a range of pr-proteins, through heterologous expression in transgenic carrot tissues, for their ... | 2006 | 17033046 |
| accumulation of p-hydroxybenzoic acid in hairy roots of daucus carota. | we report here the accumulation of p-hydroxybenzoic acid in agrobacterium rhizogenes-induced hairy root cultures of daucus carota. this phenolic acid finds application in food, pharmaceutical and polymer industries. metabolic profiling of phenolics by hplc/esi-ms from these hairy roots showed a considerable amount of p-hydroxybenzoic acid accumulation both in cytosol and in the cell wall. analyses of hcl and naoh treated soluble phenolic fractions resulted in the elution of peaks with same reten ... | 2007 | 17027117 |
| the dcmaster transposon display maps polymorphic insertion sites in the carrot (daucus carota l.) genome. | dcmaster is a family of pif/harbinger-like class ii transposable elements identified in carrot. we present a modified transposon display molecular marker system allowing amplification of genomic regions containing dcmaster elements. we scored 77 dcmaster transposon display (dcmtd) amplicons, of which 54 (70%) were segregating in the f(2) progeny from the cross between wild and cultivated carrot. segregating amplicons were incorporated into a previously developed molecular linkage map of carrot. ... | 2007 | 17011731 |
| complete plastid genome sequence of daucus carota: implications for biotechnology and phylogeny of angiosperms. | carrot (daucus carota) is a major food crop in the us and worldwide. its capacity for storage and its lifecycle as a biennial make it an attractive species for the introduction of foreign genes, especially for oral delivery of vaccines and other therapeutic proteins. until recently efforts to express recombinant proteins in carrot have had limited success in terms of protein accumulation in the edible tap roots. plastid genetic engineering offers the potential to overcome this limitation, as dem ... | 2006 | 16945140 |
| kdc2, a functional homomeric potassium channel expressed during carrot embryogenesis. | in daucus carota, the model system for embryogenesis, it has been demonstrated that potassium and k(+) selective channels are involved in embryo development. here, we report the isolation and cloning of a new carrot shaker-like potassium channel, potassium d. carota channel 2 (kdc2), whose expression pattern during somatic embryogenesis proceeds along with the establishment of the polar axes and the settlement of the hypocotyl region. in plants, kdc2 transcript is localized at the shoot level, i ... | 2006 | 16934807 |
| stoichiometry studies reveal functional properties of kdc1 in plant shaker potassium channels. | functional heteromeric plant shaker potassium channels can be formed by the assembly of subunits from different tissues, as well as from diverse plant species. kdc1 (k(+) daucus carota 1) produces inward-rectifying currents in xenopus oocytes when coexpressed with kat1 and other subunits appertaining to different plant shaker subfamilies. owing to the presence of kdc1, resulting heteromeric channels display slower activation kinetics, a shift of the activation threshold toward more negative memb ... | 2006 | 16920836 |
| seed germination in response to chemicals: effect of nitrogen and ph in the media. | seed germination generally presents a peak in the next growing season after a fire. among other factors associated with fire are the increase of soil nitrogen and changes in the ph of the soil. in this study, we addressed the question, whether or not the germination response of eight species is linked with the increase in ph and nitrogenous compounds in the germination media? we assessed the separate and combined effects of nitrogenous compounds and ph on the percentage and rate of germination o ... | 2006 | 16850869 |
| daucus carota l. mediated bioreduction of prochiral ketones. | stereoselective reductions of ketones to secondary alcohols are of the utmost importance in organic synthesis. very high selectivities are observed with traditional reducing agents, mainly based on boron or transition metals, complexed with chiral ligands. bioreductions mediated by intact cells from cut plants, vegetables and fruits are attractive alternatives and could facilitate transition towards a more biobased economy. this emerging area highlights the recent results obtained in the aqueous ... | 2006 | 16763677 |
| pharmacological evidence for the potential of daucus carota in the management of cognitive dysfunctions. | the present study was aimed at investigating the effects of daucus carota seeds on cognitive functions, total serum cholesterol levels and brain cholinesterase activity in mice. the ethanolic extract of daucus carota seeds (dce) was administered orally in three doses (100, 200, 400 mg/kg) for seven successive days to different groups of young and aged mice. elevated plus maze and passive avoidance apparatus served as the exteroceptive behavioral models for testing memory. diazepam-, scopolamine- ... | 2006 | 16755009 |
| effect of p availability on temporal dynamics of carbon allocation and glomus intraradices high-affinity p transporter gene induction in arbuscular mycorrhiza. | arbuscular mycorrhizal (am) fungi depend on a c supply from the plant host and simultaneously provide phosphorus to the colonized plant. we therefore evaluated the influence of external p on c allocation in monoxenic daucus carota-glomus intraradices cultures in an am symbiosis. fungal hyphae proliferated from a solid minimal medium containing colonized roots into a c-free liquid minimal medium with high or low p availability. roots and hyphae were harvested periodically, and the flow of c from ... | 2006 | 16751522 |
| reduced expression of top1beta gene induces programmed cell death and alters ascorbate metabolism in daucus carota cultured cells. | topoisomerase i (topo i) is a nuclear enzyme which plays a fundamental role in several pathways involving changes in dna topology. the topo i-mediated reaction is accomplished by the transient covalent binding of the enzyme to dna (topo i-dna complex). stabilization of the topo i-dna complex, leading to irreversible double-strand breaks, has been reported to occur in animal cells under oxidative stress conditions and during apoptosis. in order to study the existence of a putative link between th ... | 2006 | 16717060 |
| tissue-specific accumulation of carotenoids in carrot roots. | raman spectroscopy can be used for sensitive detection of carotenoids in living tissue and raman mapping provides further information about their spatial distribution in the measured plant sample. in this work, the relative content and distribution of the main carrot (daucus carota l.) root carotenoids, alpha-, beta-carotene, lutein and lycopene were assessed using near-infrared fourier transform raman spectroscopy. the pigments were measured simultaneously in situ in root sections without any p ... | 2006 | 16699778 |
| differential accumulation of biotin enzymes during carrot somatic embryogenesis. | the activities of four biotin enzymes, acetyl-coenzyme a (coa) carboxylase, 3-methylcrotonyl-coa carboxylase, pyruvate carboxylase, and propionyl-coa carboxylase, and the accumulation of six biotin-containing polypeptides were determined during development of somatic embryos of carrot (daucus carota). acetyl-coa carboxylase activity increased more than sevenfold, whereas the activities of 3-methylcrotonyl-coa carboxylase, pyruvate carboxylase, and propionyl-coa carboxylase were relatively unalte ... | 1992 | 16669096 |
| a wound-inducible glycine-rich protein from daucus carota with homology to single-stranded nucleic acid-binding proteins. | a cdna clone was isolated that encodes a wound-inducible glycine-rich protein. the homology of the carrot (daucus carota) protein with the a1 protein of the heterogeneous ribonucleoprotein complex suggests that the polypeptide plays a role in the biosynthesis and processing of heterogeneous nuclear rna and in the maturation of specific mrnas in response to wounding. | 1992 | 16669093 |
| immunological detection of acetaldehyde-protein adducts in ethanol-treated carrot cells. | polyclonal antibodies able to recognize protein-acetaldehyde conjugates were produced and characterized. the antibodies react with sodium cyanoborohydride-reduced schiff's bases between acetaldehyde and a protein, independently of the nature of the macromolecule binding the acetaldehyde moiety. only conjugates between acetaldehyde or propionaldehyde and a protein are recognized; conjugates obtained with other aldehydes are not reactive. results concerning the formation of acetaldehyde adducts wi ... | 1992 | 16668764 |
| regulation of glutamate dehydrogenase activity in relation to carbon limitation and protein catabolism in carrot cell suspension cultures. | glutamate dehydrogenase (gdh) specific activity and function have been studied in cell suspension cultures of carrot (daucus carota l. cv chantenay) in response to carbon and nitrogen supply in the culture medium. the specific activity of gdh was derepressed in sucrose-starved cells concomitant with protein catabolism, ammonium excretion, and the accumulation of metabolically active amino acids. the addition of sucrose led to a rapid decrease in gdh specific activity, an uptake of ammonium from ... | 1992 | 16668745 |
| calcium-pumping atpases in vesicles from carrot cells : stimulation by calmodulin or phosphatidylserine, and formation of a 120 kilodalton phosphoenzyme. | ca(2+)-atpases keep cytoplasmic [ca(2+)] low by pumping ca(2+) into intracellular compartments or out of the cell. the transport properties of ca(2+)-pumping atpases from carrot (daucus carota cv danvers) tissue culture cells were studied. atp-dependent ca(2+) transport in vesicles that comigrated with an endoplasmic reticulum marker, was stimulated three- to fourfold by calmodulin. cyclopiazonic acid (a specific inhibitor of the sarcoplasmic/endoplasmic reticulum ca(2+)-atpase) partially inhibi ... | 1991 | 16668581 |
| bifunctional protein in carrot contains both aspartokinase and homoserine dehydrogenase activities. | we have purified homoserine dehydrogenase to homogeneity and subjected polypeptide fragments derived from digests of the protein to amino acid sequencing. the amino acid sequence of homoserine dehydrogenase from carrot (daucus carota) indicates that in carrot both aspartokinase and homoserine dehydrogenase activities reside on the same protein. additional evidence that aspartokinase and homoserine dehydrogenase reside on a bifunctional protein is provided by coelution of activities during purifi ... | 1991 | 16668550 |
| rapid purification and thermostability of the cytoplasmic aspartate aminotransferase from carrot suspension cultures. | several isoenzymic forms of aspartate aminotransferase (aat) have been identified in protein extracts from carrot (daucus carota) cell suspension cultures. the cellular location of the major form (form i) of aat in carrot suspension cultures was determined by heat inactivation, subcellular fractionation, and amino acid sequence analysis. in mammalian systems, there are two forms of aat, a heat-stable cytoplasmic form and a heat-labile form in the mitochondria. the thermostability of three isoenz ... | 1991 | 16668442 |
| modulation of auxin-binding proteins in cell suspensions : i. differential responses of carrot embryo cultures. | this paper shows that the level of 2,4-dichlorophenoxyacetic acid (2,4-d) in the medium determines the level of auxin-binding proteins in the membranes of carrot, daucus carota, cells grown in suspension. this induction takes slightly more than 2 hours to complete and can be elicited by natural as well as synthetic auxins. the auxin binding sites thus generated, which are pronase-sensitive, bind 2,4-d, indoleacetic acid, and naphthalene-acetic acid (naa) equally well. however both alpha- and bet ... | 1991 | 16668416 |
| heterogeneity and cell type-specific localization of a cell wall glycoprotein from carrot suspension cells. | ep1, an extracellular protein from carrot (daucus carota) cell suspensions, has been partially characterized by means of an antiserum and a cdna clone. in both embryo and suspension cultures different molecular mass ep1 proteins were detected, some of which (31, 32, 52, and 54 kilodaltons) were bound to the cell wall and released into the medium, whereas others (49, 60, and 62 kilodaltons) were more firmly bound to the cell wall and could be extracted with a salt solution. immunoprecipitation of ... | 1991 | 16668246 |
| purification and immunohistochemical detection of an embryogenic cell protein in carrot. | an embryogenic cell protein from carrot (daucus carota l.), designated ecp31 for embryogenic cell protein and with a relative mass of 31,000, was purified by sequential column chromatographies. its apparent relative mass was estimated to be 120,000 by gel filtration. immunoblotting and immunohistochemical studies showed that ecp31 was preferentially localized in the peripheral cells of clusters of embryogenic cells in the presence of 2,4-dichlorophenoxyacetic acid (2,4-d) and disappeared during ... | 1991 | 16668093 |
| ethanol-induced injuries to carrot cells : the role of acetaldehyde. | carrot (daucus carota l.) cell cultures show high sensitivity to ethanol since both unorganized cell growth and somatic embryogenesis are strongly inhibited by ethanol at relatively low concentrations (10-20 millimolar). the role of acetaldehyde on ethanol-induced injuries to suspension cultured carrot cells was evaluated. when ethanol oxidation to acetaldehyde is prevented by adding an alcohol-dehydrogenase (ec 1.1.1.1) inhibitor (4-methylpyrazole) to the culture medium, no ethanol toxicity was ... | 1991 | 16668049 |
| the role of glutamate dehydrogenase in plant nitrogen metabolism. | in vivo nuclear magnetic resonance spectroscopy, in vitro gas chromatography-mass spectrometry, and automated (15)n/(13)c mass spectrometry have been used to demonstrate that glutamate dehydrogenase is active in the oxidation of glutamate, but not in the reductive amination of 2-oxogiutarate. in cell suspension cultures of carrot (daucus carota l. cv chantenay), primary assimilation of ammonium occurs via the glutamate synthase pathway. glutamate dehydrogenase is derepressed in carbonlimited cel ... | 1991 | 16668014 |
| conditions favorable for the somatic embryogenesis in carrot cell culture enhance expression of the roic promoter-gus fusion gene. | we obtained carrot (daucus carota) cells possessing the 5'-noncoding sequence of the orf12 gene (roic) of tl-dna of the ri plasmid and a structural gene of bacterial beta-glucuronidase by agrobacterium-mediated transformation. when such cells were cultured in medium containing 2,4-dichlorophenoxyacetic acid, substantial reduction in beta-glucuronidase activity was observed. upon transferring the cells from a 2,4-d-containing medium to one devoid of 2,4-dichlorophenoxyacetic acid, enhanced expres ... | 1991 | 16667958 |
| specific levels of dna methylation in various tissues, cell lines, and cell types of daucus carota. | the level of dna methylation in daucus carota was found to be tissue specific, but no simple correlation between developmental stage or age of tissue and the level of dna methylation was found. among three different suspension culture lines from the same variety grown under identical conditions, large differences in the level of dna methylation were observed. the highest and lowest levels were found in two embryogenic cell lines originating from the same clone. suspension cells from one of the e ... | 1991 | 16667947 |
| short-term treatment with cell wall degrading enzymes increases the activity of the inositol phospholipid kinases and the vanadate-sensitive atpase of carrot cells. | treating carrot (daucus carota l.) suspension culture cells with a mixture of cell wall degrading enzymes, driselase, resulted in an increase in the percentage of [(3)h]phosphatidylinositol bisphosphate. analysis of the lipid kinase activities in the isolated plasma membranes after whole cell treatment indicated that treatment with driselase (2% weight/volume; the equivalent of 340 units per milliliter of hemicellulase and 400 units per milliliter of cellulase activity) or treatment with hemicel ... | 1990 | 16667922 |
| abscisic acid regulation of dc8, a carrot embryonic gene. | dc8 encodes a hydrophylic 66 kilodalton protein located in the cytoplasm and cell walls of carrot (daucus carota) embryo and endosperm. during somatic embryogenesis, the levels of dc8 mrna and protein begin to increase 5 days after removal of auxin. to study the role of abscisic acid (aba) in the regulation of dc8 gene, fluridone, 1-methyl-3-phenyl,-5(3-trifluoro-methyl-phenyl)-4(1h)-pyridinone, was used to inhibit the endogenous aba content of the embryos. fluridone, 50 micrograms per millilite ... | 1990 | 16667766 |
| purification and some properties of a daucus carota lectin which enhances the activation of prophenoloxidase by cacl(2). | carrot (daucus carota) cell cultures were found to secrete a protein, with the molecular weight of 58,000, which could strongly enhance the activation of carrot prophenoloxidase by cacl(2). the protein was isolated from the culture filtrate and purified to electrophoretic homogeneity. the purified protein did also show hemagglutinating activity toward rat as well as rabbit erythrocytes and this activity was inhibitied by n-acetylglucosamine or fetuin. | 1990 | 16667519 |
| the s-methylmethionine cycle in lemna paucicostata. | the metabolism of s-methylmethionine has been studied in cultures of plants of lemna paucicostata and of cells of carrot (daucus carota) and soybean (glycine max). in each system, radiolabeled s-methylmethionine was rapidly formed from labeled l-methionine, consistent with the action of s-adenosyl-l-methionine:methionine s-methyltransferase, an enzyme which was demonstrated during these studies in lemna homogenates. in lemna plants and carrot cells radiolabel disappeared rapidly from s-methylmet ... | 1990 | 16667513 |