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partial sequence analysis of an atypical turkish isolate provides further information on the evolutionary history of plum pox virus (ppv).a variety of techniques, such as typing with subgroup-specific monoclonal antibodies, restriction length polymorphism (rflp) analysis or subgroup-specific rt-pcr are available for the discrimination of plum pox virus (ppv) isolates. however, the existence of ppv isolates showing abnormal typing properties has been observed in the past [candresse, t., cambra, m., dallot, s., lanneau, m., asensio, m., gorris, m.t., revers, f., macquaire, g., olmos, a., boscia, d., quiot j.b., dunez, j., 1998. comp ...200515681071
general-purpose infusion pumps: evaluating the hospira plum a+ with mednet.this study updates ecri's previous evaluations of general-purpose infusion pumps, most notably those published in the october 2002 and october 2003 health devices. it features the results of our findings for the new dose error reduction system now included with the hospira (formerly abbott) plum a+ with mednet, and it includes updated information for the previously evaluated models that also include this safety feature. general-purpose infusion pumps allow the controlled delivery of liquid medic ...200415675737
phenology and infestation patterns of plum curculio (coleoptera: curculionidae) on four highbush blueberry cultivars.the plum curculio, conotrachelus nenuphar (herbst), is a well known pest in apple and peach orchards, but it also is capable of having an economic impact in highbush blueberries. host phenology and plum curculio oviposition patterns were determined on four highbush blueberry cultivars differing in fruit maturation period. numbers of oviposition scars were higher on early- ('weymouth') and mid-season ('duke' and 'bluecrop') blueberries than on late-season 'elliott' in 2001, 2002, and 2003. in 200 ...200415666742
analogues of virus resistance genes map to qtls for resistance to sharka disease in prunus davidiana.plum pox virus (ppv), the causative agent of sharka disease in prunoideae, is one of the most serious problems affecting stone fruit production in europe and america. resistance to ppv was previously described in a prunus davidiana clone, p1908, and introduced into peach (prunus persica) genotypes. genetic resistance to ppv displays a complex pattern of quantitative inheritance. an analysis of quantitative trait loci (qtls) for resistance was performed on an f1 interspecific peach population obt ...200515666162
simultaneous detection of six stone fruit viruses by non-isotopic molecular hybridization using a unique riboprobe or 'polyprobe'.a new strategy for the simultaneous detection of plant viruses by molecular hybridization has been developed. two, four or six viral sequences were fused in tandem and transcribed to render unique riboprobes and designated as 'polyprobes'. the 'polyprobe four' (poly 4) covered the four ilarviruses affecting stone fruit trees including apple mosaic virus (apmv), prunus necrotic ringspot virus (pnrsv), prune dwarf virus (pdv), and american plum line pattern virus (aplpv) whereas the 'polyprobe two ...200415664050
cutaneous tumors as the initial presentation of non-t, non-b, nonmyeloid cd4+ cd56+ hematolymphoid malignancy in an adolescent boy.we describe a 14-year-old hispanic boy who presented with a 2-month history of enlarging plum-colored cutaneous tumors on his face, trunk, and proximal extremities. histopathologic examination showed nodular infiltrates of malignant mononuclear cells extending from the superficial dermis to the deep subcutis. immunohistochemical staining of the biopsy specimen and flow cytometry studies on a bone marrow aspirate revealed a cd4+, cd56+ hematolymphoid tumor that was negative for all other myeloid ...200515660891
executive government positions of influence in biodefense: the bio-plum book. 200415650442
linkage map positions and allelic diversity of two mal d 3 (non-specific lipid transfer protein) genes in the cultivated apple (malus domestica).non-specific lipid transfer proteins (nsltps) of rosaceae fruits, such as peach, apricot, cherry, plum and apple, represent major allergens for mediterranean atopic populations. as a first step in elucidating the genetics of nsltps, we directed the research reported here towards identifying the number and location of nsltp (mal d 3) genes in the apple genome and determining their allelic diversity. pcr cloning was initially performed on two cultivars, prima and fiesta, parents of a core apple ma ...200515647923
antioxidant properties of prunes (prunus domestica l.) and their constituents.prunes contain large amounts of phenolics and show high antioxidant activity. the aim of this study is to clarify the contents of caffeoylquinic acid (cqa) isomers, and to estimate the contribution of these isomers to the antioxidant activity of prunes. furthermore, structural elucidation and evaluation of antioxidant activity of prune components were also performed. cqa isomers in prunes were quantified by hplc analysis, and it has become apparent that prunes contain relatively high amount of 4 ...200415630217
detection and differentiation of plum pox virus using real-time multiplex pcr with sybr green and melting curve analysis: a rapid method for strain typing.a real-time multiplex pcr procedure with melting curve analysis, using the green fluorescence dye sybr green i, was developed for rapid and reliable identification of plum pox virus (ppv) isolates of strains d and m. members of the different strains were identified by their distinctive melting temperatures (t(m)s); 84.3-84.43 degrees c for d isolates, and 85.34-86.11 degrees c for m isolates. the associated amplicon sizes were 114 and 380 bp, respectively. the procedure was used for detection an ...200515620404
[osmolality of frequently consumed beverages].the objective of this work was to determine the osmolality of beverages frequently consumed by children and adolescents due to the scarce information available in our country. the samples were grouped as follows: milks; refreshments; beverages based on fruits, vegetables, cereals, and tubers; sport drinks; energizing drinks; oral rehydrating solutions; reconstituted drinks and infusions. a vapor pressure digital osmometer was used, five samples of each beverage from different lots were analyzed. ...200415602899
analysis of shaking beam actuator for piezoelectric linear ultrasonic motor.in this paper, piezoelectric linear ultrasonic motors (plum) have been investigated on the elliptic trajectory of a contact point in shaking beam, which has been accomplished by two resonance vibration modes of the actuators. the actuators have generated the vibration modes, longitudinal and flexural, by two longitudinal mechanical vibrations with phase difference of pi/2. modal and harmonic analysis of the shaking beam actuator were performed by the finite element method (fem) to calculate a re ...200415600096
a simplified rt-pcr-based detection of recombinant plum pox virus isolates.closely related natural plum pox virus (ppv) isolates derived from homologous rna recombination between ppv-d and ppv-m have been recently identified and shown naturally spread in several european countries. as their serological properties were identical with those of conventional ppv-m isolates, they could be detected only by combined analysis of at least two different genome parts. to simplify the detection of such recombinants primers specific to ppv-m and ppv-d sequences with binding sites l ...200415595211
evaluation of hydrophobic and hydrophilic kaolin particle films for peach crop, arthropod and disease management.hydrophobic and/or hydrophilic kaolin particle film treatments to peach (prunus persica (l) batsch) trees were evaluated for crop and pest management capabilities in six studies from 1997 to 2000. unsprayed control and standard treatments, the latter consisting of a commercial pesticide program, were included for comparison. treatments in initial studies were applied via handgun, which resulted in a uniform and heavy deposit of kaolin after the first application. in contrast, treatments in subse ...200515593071
interaction of amphetamine and its n-alkyl-substituted derivatives with micro- and mesoporous adsorbents in polar liquids.the process of concentrating amphetamine (1-phenyl-2-propanamine, c6h5ch2ch(nh2)ch3) and its n-alkyl substituted derivatives c6h5ch2ch(nhr)ch3 and c6h5ch2ch(n(ch3)r)ch3 (r=(ch2)(n)ch3 at n=0, 1, 2, and 3) from diluted aqueous solution was investigated using six adsorbents having different textures and chemical compositions. three chemically modified carbon adsorbents prepared from plum stones and routinely used spe cartridges packed with graphitized adsorbents such as hypercarb and envicarb and ...200515589530
stability of gene silencing-based resistance to plum pox virus in transgenic plum (prunus domestica l.) under field conditions.plum pox virus (ppv) is one of the most devastating diseases of prunus species. since few sources of resistance to ppv have been identified, transgene-based resistance offers a complementary approach to developing ppv-resistant stone fruit cultivars. c5, a transgenic clone of prunus domestica l., containing the ppv coat protein (cp) gene, has been described as highly resistant to ppv in greenhouse tests, displaying characteristics typical of post-transcriptional gene silencing (ptgs). we show in ...200415587267
inhibition of cancer cell proliferation in vitro by fruit and berry extracts and correlations with antioxidant levels.the effects of 10 different extracts of fruits and berries on cell proliferation of colon cancer cells ht29 and breast cancer cells mcf-7 were investigated. the fruits and berries used were rosehips, blueberries, black currant, black chokeberries, apple, sea buckthorn, plum, lingonberries, cherries, and raspberries. the extracts decreased the proliferation of both colon cancer cells ht29 and breast cancer cells mcf-7, and the effect was concentration dependent. the inhibition effect for the high ...200415563205
is there a scientific basis for the therapeutic effects of serenoa repens in benign prostatic hyperplasia? mechanisms of action.the huge resurgence of interest in herbal remedies has spawned a global industry that now competes with conventional drugs as adjuncts and/or alternatives for various conditions. the medical treatment of benign prostatic hyperplasia (bph) is no exception. along with alpha-blockers and 5alpha-reductase inhibitors the extract of the american dwarf palm, serenoa repens, is unquestionably the most widely used. together with pygeum africanum, an extract from the bark of the african plum tree, it is l ...200415540722
sublethal toxicity of orchard stormwater runoff in sacramento splittail (pogonichthys macrolepidotus) larvae.the sublethal effects of stormwater runoff from sections of a plum orchard treated with esfenvalerate or diazinon were evaluated in 7-day-old sacramento splittail (pogonichthys macrolepidotus) larvae. fish were exposed to eight runoff samples using the usepa standard static renewal method for 96 h acute toxicity testing, then transferred to clean water for three-month to assess the survival, growth, histopathological abnormalities, and heat stress proteins (hsp). no significant mortality was obs ...200515465130
exposure time of oral rabies vaccine baits relative to baiting density and raccoon population density.oral rabies vaccination (orv) baiting programs for control of raccoon (procyon lotor) rabies in the usa have been conducted or are in progress in eight states east of the mississippi river. however, data specific to the relationship between raccoon population density and the minimum density of baits necessary to significantly elevate rabies immunity are few. we used the 22-km2 us national aeronautics and space administration plum brook station (pbs) in erie county, ohio, usa, to evaluate the per ...200415362821
inhibitory effects of cystatins on proteolytic activities of the plum pox potyvirus cysteine proteinases.in an effort to develop new antiviral strategies effective against potyviruses, several cystatins were evaluated for their ability to inhibit the cysteine proteinases of plum pox potyvirus (ppv) using in vitro proteolytic assays. the following cystatins were purified as gst fusion proteins and shown to be active against papain:oryzacystatins i and ii (oci and ocii), corn cystatin ii (ccii), human stefin a (hsa), the domain 8 of tomato multicystatin (tmc-8) and a large 24kda tomato cystatin (ltcy ...200415351491
high-resolution mapping and chromosome landing at the root-know nematode resistance locus ma from myrobalan plum using a large-insert bac dna library.the ma gene for root-knot nematode (rkn)resistance from myrobalan plum (prunus cerasifera l.)confers a complete-spectrum and a heat-stable resistance to meloidogvne spp., conversely to mi-i from tomato,which has a more restricted spectrum and a reduced efficiency at high temperature. this gene was identified from a perennial self-incompatible near-wild rootstock species and lies in cosegregation with the scar marker scaflp2 on the prunus linkage group 7 in a 2.3 cm interval between the scar scal ...200415322755
'core knowledges': a dissociation between spatiotemporal knowledge and contact-mechanics in a non-human primate?human toddlers demonstrate striking failures when searching for hidden objects that interact with other objects, yet successfully locate hidden objects that do not undergo mechanical interactions. this pattern hints at a developmental dissociation between contact-mechanical and spatiotemporal knowledge. recent studies suggest that adult non-human primates may exhibit a similar dissociation. here, i provide the first direct test of this dissociation using a search paradigm with adult rhesus monke ...200415320376
[test for urokinase-type plasminogen activator inhibitor of edible plants in vitro].to study the effect of anti-progression activity of edible plants using urokinase as the biomarker.200415312585
geographically and temporally distant natural recombinant isolates of plum pox virus (ppv) are genetically very similar and form a unique ppv subgroup.natural recombinant plum pox virus (ppv) isolates were detected in albania, bulgaria, czech republic, germany, hungary and slovakia. despite different geographical origins and dates of isolation, all the recombinant isolates were closely related at the molecular level and shared the same recombination breakpoint as well as a typical signature in their n-terminal coat protein sequence, suggesting a common origin. biological assays with four recombinant isolates demonstrated their capacity to be a ...200415302961
electrochemical determination of triple helices: electrocatalytic oxidation of guanine in an intramolecular triplex.electrocatalytic oxidation of the oligonucleotide 5'- gaa gag gtt ttt cct ctt ctt ttt ctt ctc c (ts) by ru(bpy)(3)(2+) was studied by cyclic voltammetry. this oligonucleotide forms either an intramolecular triplex, hairpin, or single strand, depending on the ph (plum, g. e.; breslauer, k. j. j. mol. biol. 1995, 248, 679-695). in the triplex form, the guanine doublet in ts is buried inside the folded structure, and as such is less susceptible to oxidation by electrogenerated ru(bpy)(3)(3+). digit ...200415285684
african plum and benign prostatic hypertrophy. 200415273076
microsatellite genetic linkage maps of myrobalan plum and an almond-peach hybrid--location of root-knot nematode resistance genes.inheritance and linkage studies were carried out with microsatellite [or simple sequence repeat (ssr)] markers in a f(1) progeny including 101 individuals of a cross between myrobalan plum ( prunus cerasifera ehrh) clone p.2175 and the almond (prunus dulcis mill.)-peach ( prunus persica l. batsch) hybrid clone gn22 ["garfi" (g) almond x "nemared" (n) peach]. this three-way interspecific prunus progeny was produced in order to associate high root-knot nematode (rkn) resistances from myrobalan and ...200415241595
specific detection and quantification of plum pox virus by real-time fluorescent reverse transcription-pcr.plum pox virus (ppv), a destructive and economically devastating pathogen of prunus species, was recently discovered in pennsylvania and canada. current containment efforts involve eradication of infected trees based on elisa surveys, which are laborious and less sensitive than pcr-based techniques. a real-time, fluorescent, reverse transcription-polymerase chain reaction (rt-pcr) assay was developed for the detection of ppv in the smart cycler (cepheid). the methods developed are reproducible, ...200415234814
xylella fastidiosa subspecies: x. fastidiosa subsp. [correction] fastidiosa [correction] subsp. nov., x. fastidiosa subsp. multiplex subsp. nov., and x. fastidiosa subsp. pauca subsp. nov.xylella fastidiosa, a fastidious bacterium causing disease in over 100 plant species, is classified as a single species, although genetic studies support multiple taxons. to determine the taxonomic relatedness among strains of x. fastidiosa, we conducted dna-dna relatedness assays and sequenced the 16s-23s intergenic spacer (its) region using 26 strains from 10 hosts. under stringent conditions (tm -15 degrees c), the dna relatedness for most x. fastidiosa strains was *70%. however, at high stri ...200415214634
effect of the epicuticular waxes of fruits and vegetables on the photodegradation of rotenone.the effect of epicuticular waxes extracted from fruits (apple, nectarine, pear, and plum) and vegetables (tomato and eggplant) on the photodegradation of rotenone was studied. the waxes affected the decay rate and the degradation pathway of this botanical insecticide. tomato, nectarine, and plum waxes decreased the photodegradation rate compared to controls, whereas apple and pear waxes increased it. rotenone irradiated under sunlight without waxes gave seven photoproducts; in contrast, in the p ...200415161214
validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative pcr detection of transgenes.with the development of transgenic crops, many countries have issued regulations to label the genetically modified organisms (gmos) and their derived products. polymerase chain reaction (pcr) methods are thought to be reliable and useful techniques for qualitative and quantitative detection of gmos. these methods generally need to amplify the transgene and compare the amplified result with that of the corresponding reference gene to obtain reliable results. in this article, we reported the devel ...200415161200
incidence and control of dogwood borer (lepidoptera: sesiidae) and american plum borer (lepidoptera: pyralidae) infesting burrknots on clonal apple rootstocks in new york.surveys were conducted in the major apple growing regions of new york state to determine the incidence of borers infesting burrknots on clonal apple rootstocks. dogwood borer, synanthedon scitula (harris), was generally prevalent throughout the state, but american plum borer, euzophera semifuneralis (walker), was limited to western new york apple orchards near infested stone fruit trees. insecticides evaluated in the field for efficacy against both borers were chlorpyrifos, endosulfan, indoxacar ...200415154480
3 using odor-baited trap trees as sentinels to monitor plum curculio (coleoptera: curculionidae) in apple orchards.in commercial orchards in massachusetts in 2003, we conducted experiments aimed at developing guidelines for use of perimeter-row trap trees baited with grandisoic acid plus benzaldehyde as sentinels in a practical approach to determining need and timing of insecticide applications against overwintered plum curculios, conotrachelus nenuphar (herbst). evaluations were based on percentages of sampled fruit injured by plum curculio. trap trees baited with grandisoic acid released at approximately 1 ...200415154475
comparison of a sprayable pheromone formulation and two hand-applied pheromone dispensers foruse in the integrated control of oriental fruit moth (lepidoptera: tortricidae).the efficacy of integrated programs using a sprayable pheromone formulation or one of two hand-applied pheromone dispensers, and a conventional oriental fruit moth, grapholita molesta (busck) (lepidoptera: tortricidae) control program, was compared using 4-5-ha blocks of peach orchard at three niagara peninsula farms during 2000-2002. in the integrated programs, chlorpyrifos and mating disruption with 3m sprayable pheromone, isomate ofm rosso, or rak 5 hand-applied dispensers were used to contro ...200415154471
a new bipyrrole and some phenolic constituents in prunes (prunus domestica l.) and their oxygen radical absorbance capacity (orac).isolation and structural elucidation of prune constituents were performed and total 10 compounds were determined by nmr and ms analyses. a novel compound was identified to be 2-(5-hydroxymethyl-2',5'-dioxo-2',3',4',5'-tetrahydro-1'h-1,3'-bipyrrole)carbaldehyde, and 7 phenolic compounds were isolated from prunes for the first time. in addition, antioxidant activity of them was evaluated on the basis of the oxygen radical absorbance capacity (orac).200415118329
volatile flavor constituents of fruits from southern africa: mobola plum (parinari curatellifolia).the volatile flavor components of mobola plum (parinari curatellifolia), a native fruit of southern africa, have been isolated by a vacuum headspace concentration method. the concentrate was analyzed by hyphenated gas chromatographic techniques, including gas chromatography (gc)/mass spectrometry (ms) and gc/fourier transform infrared (ftir), together with other gc-coupled detection devices for the specific and selective detection of nitrogen- and sulfur-containing compounds. a total of 88 compo ...200415080640
isolation and phylogenetic analysis of xylella fastidiosa from its invasive alternative host, porcelain berry.a strain of xylella fastidiosa was isolated from an invasive alternative host species, porcelain berry. its genetic relationship with strains isolated from a native alternative host, wild grape; a nonnative alternative host, mulberry; and other economically important hosts including cultivated grape, peach, plum, oak, maple and oleander was determined by using sequence analysis of the 16s-23s rrna intergenic spacer region. our phylogenetic analysis revealed that the porcelain berry strain is mos ...200415018107
microbial biogeography along an estuarine salinity gradient: combined influences of bacterial growth and residence time.shifts in bacterioplankton community composition along the salinity gradient of the parker river estuary and plum island sound, in northeastern massachusetts, were related to residence time and bacterial community doubling time in spring, summer, and fall seasons. bacterial community composition was characterized with denaturing gradient gel electrophoresis (dgge) of pcr-amplified 16s ribosomal dna. average community doubling time was calculated from bacterial production ([(14)c]leucine incorpor ...200415006771
monitoring plum curculio, conotrachelus nenuphar (coleoptera: curculionidae), populations in apple and peach orchards in the mid-atlantic.we evaluated responses of plum curculio, conotrachelus nenuphar (herbst), to four trap types in commercial and unsprayed apple and peach orchards. trap types included black pyramid and clear plexiglas panel traps deployed outside the orchard 2 m from the border row, and branch-mimicking cylinder and trunk-mounted screen traps attached to trees in the border row. bait treatments evaluated in conjunction with each trap type included the synthetic fruit volatile benzaldehyde, the aggregation pherom ...200414998130
reproductive development of northern and southern strains of plum curculio (coleoptera: curculionidae).laboratory-reared southern and field-collected northern strains of plum curculio, conotrachelles nenuphar (herbst), were sampled to examine the relationship between degree-day (dd) accumulation and female reproductive development, as measured by mating status, oocyte size, and number of oocytes. the overall goal was to generate an objective degree-day model for predicting damage potential that could be applied to various host commodities rather than relying on separate biofix models for each cro ...200414998124
growth and the composition and transport of carbohydrate in compatible and incompatible peach/plum grafts.the growth of scions and rootstocks of compatible (prunus persica l. batsch cv. springtime/prunus cerasifera l. ehrh. cv. myrobolan p2032) and incompatible (prunus persica l. batsch cv. springtime/prunus cerasifera l. ehrh. cv. myrobolan p18) peach/plum grafts were compared. the composition of soluble carbohydrates in phloem and cortical tissues of both peach/plum grafts and ungrafted plums and the translocation of these compounds across the union of grafted plants were examined. sorbitol and su ...198714975918
the effect of different atmospheric ozone partial pressures on photosynthesis and growth of nine fruit and nut tree species.nursery stock of peach (prunus persica l. batsch, cv. o'henry), nectarine (p. persica l. batsch, cv. fantasia), plum (p. salicina lindel., cv. casselman), apricot (p. armeniaca l., cv. tilton), almond (p. dulcis mill., cv. nonpareil), prune (p. domestica l., cv. improved french), cherry (p. avium l., cv. bing), oriental pear (pyrus pyrifolia rehd., cv. 20th century), and apple (malus pumula mill., cv. granny smith) were planted in open-top chambers on april 1, 1988 at the university of californi ...199114972900
growth, cambial activity and phloem structure in compatible and incompatible peach/plum grafts.peach (prunus persica l. batsch) shoots grafted onto compatible or incompatible clones of rootstocks of myrobolan plum (prunus cerasifera l. ehrh. cv. myrobolan) were observed in a greenhouse for 100 days after grafting. the incompatible grafts showed foliar symptoms of incompatibility and reduced shoot growth about 60 days after grafting. light microscopic studies revealed that cambial activity stopped earlier in the rootstocks, than in the scions, of incompatible grafts. structural modificatio ...198814972805
comparison of drought resistance among prunus species from divergent habitats.root and shoot characteristics related to drought resistance were compared among cultivated peach (prunus persica (l.) batsch.), p. andersonii (nevada desert almond), p. besseyi (western sand cherry), p. maritima (beach plum), p. subcordata (sierra or pacific plum), and p. tomentosa (nanking cherry). in all species, shoot characteristics were more closely associated with drought adaptation than root characteristics. the most xeric species, p. andersonii, had the lowest specific leaf area, smalle ...199214969942
carbon and nitrogen partitioning in peach/plum grafts.modifications in root-shoot relationships induced by graft incompatibility were studied in peach/plum graft combinations by means of carbohydrate and nitrogen analyses and isotope labeling. mobilization of stored carbon, phloem transport of carbon, and mobilization, assimilation and distribution of nitrogen were studied in one compatible peach/plum graft (prunus persica l. batsch cv. springtime grafted on prunus cerasifera l. ehrh cv. myrobalan p 2032) and one incompatible graft (prunus persica ...199214969877
carbohydrate composition of selected plum/prune preparations.eighteen plum/prune preparations and byproducts were analyzed for proximate constituents and carbohydrate profiles. plum puree and prune juice contained the highest concentrations of ash (13.0 and 13.8%, respectively). crude protein (cp), acid-hydrolyzed fat (ahf), and total dietary fiber (tdf) concentrations were higher in byproducts (waste cake and dried plum pits) compared with the other fractions. several classes of oligosaccharides were found in low concentrations in many of the substrates ...200414969541
field evaluation of plant odor and pheromonal combinations for attracting plum curculios.the attractiveness of different synthetic host odors and a synthetic aggregation pheromone (grandisoic acid [ga]) to overwintered adult plum curculios (pcs), conotrachelus nenuphar (herbst) (coleoptera: curculionidae), was examined using two types of traps (sticky panels and black pyramids) placed in border areas surrounding an unsprayed section of an apple orchard in massachusetts. in 2001, we evaluated the response of pcs to three synthetic fruit volatiles (benzaldehyde [ben], ethyl isovalerat ...200314969360
influence of arbuscular mycorrhizae and phosphate fertilization on shoot apical growth of micropropagated apple and plum rootstocks.we studied the effects of phosphate fertilization and inoculation with the arbuscular mycorrhizal fungi glomus mosseae (nicol. and gerd.) gerdmann and trappe, glomus intraradices schenck and smith or glomus viscosum nicolson on shoot apical growth of plantlets that had been micropropagated from mm 106 apple (malus pumila l.) and mr.s. 2/5 plum (prunus cerasifera ehrh.) rootstocks. unfertilized and non-mycorrhizal plantlets showed no apical growth during the post in vitro acclimation phase, where ...199614871682
abscisic acid related compounds and lignans in prunes (prunus domestica l.) and their oxygen radical absorbance capacity (orac).four new abscisic acid related compounds (1-4), together with (+)-abscisic acid (5), (+)-beta-d-glucopyranosyl abscisate (6), (6s,9r)-roseoside (7), and two lignan glucosides ((+)-pinoresinol mono-beta-d-glucopyranoside (8) and 3-(beta-d-glucopyranosyloxymethyl)-2- (4-hydroxy-3-methoxyphenyl)-5-(3-hydroxypropyl)-7-methoxy-(2r,3s)-dihydrobenzofuran (9)) were isolated from the antioxidative ethanol extract of prunes (prunus domestica l.). the structures of 1-4 were elucidated on the basis of nmr a ...200414733519
do we need stimulation programs as a part of nursing care for patients in "persistent vegetative state"? a conceptual analysis.the rehabilitative care of persons suffering long-lasting effects of brain injury is a significant challenge for nurses as they are the health professionals who usually spend the most time with them. historically in germany, the term "apallic syndrome" has been commonly used for what plum and posner (1980) termed the persistent vegetative state. when persons are diagnosed as being in a persistent vegetative state, that is awake but not aware, for more than six months, they seldom receive active ...200314733174
effects of foliar sprays containing calcium, magnesium and titanium on plum (prunus domestica l.) fruit quality.an experiment was performed in which ti(4+)-ascorbate was sprayed onto plum trees in several combinations with other commercial compounds containing ca2+ and mg2+ to study the effects on the commercial quality of fruits, with special focus on improving their resistance against postharvest handling damage. all the treatments containing titanium increased the tree performance (branch elongation, flowering and fruit setting intensities) and fruit size. at harvest fruits from the ti-treated trees sh ...200314717435
nutrients and antioxidant molecules in yellow plums (prunus domestica l.) from conventional and organic productions: a comparative study.yellow plums (prunus domestica l) conventionally and organically grown in the same farm were selected to study the influence of different agronomic practices on antioxidant vitamins (ascorbic acid, vitamin e, beta-carotene) and phenolics (total polyphenols, phenolic acids, flavonols) concentration. conventional plums were grown on tilled soil. three organic cultivations were performed: tilled soil, soil covered with trifolium, and soil covered with natural meadow. differences in macronutrients w ...200414709018
1-methylcyclopropene increases storability and shelf life in climacteric and nonclimacteric plums.the effect of 1-methylcyclopropene (1-mcp) at three different doses (0.25, 0.50, and 0.75 microl l(-1)) on the ripening processes of a climacteric, cv. santa rosa, and a suppressed climacteric type, cv. golden japan, plum was studied. for both cultivars, positive effects were observed in terms of inhibition of ethylene production and delays of the physical, chemical, and biochemical changes associated with ripening. 1-mcp-treated plums were firmer with lower weight loss, reduced degrees brix/tit ...200314705896
superoxide radical scavenging activity of the major polyphenols in fresh plums.the effect of polyphenols among different varieties of plums on superoxide radical scavenging activity (srsa) was studied by an enzymatic method and their ic(50) values were determined. we found that the srsa levels of the polyphenols were closely related to their chemical structures; cyanidin showed the lowest ic(50) among the polyphenols examined, and aglycones are more effective than their glycosides. by 69-339 cultivar exhibited the lowest ic(50) among the eleven plum cultivars, which means ...200314690398
ionizing irradiation quarantine treatment against plum curculio (coleoptera: curculionidae).plum curculio, conotrachelus nenuphar (herbst), is a quarantine pest of many temperate fruits, such as pomes, malus spp.; stone fruits, prunus spp.; and blueberries, vaccinium spp.; in north america east of the rocky mountains and a small area in utah. there are two strains, a northern univoltine one that undergoes obligate diapause as an adult and a southern multivoltine strain that usually has facultative diapause. current quarantine treatments for shipment to areas that do not have the pest i ...200314650511
screening of foods containing proanthocyanidins and their structural characterization using lc-ms/ms and thiolytic degradation.a normal-phase hplc-ms/ms method was applied to screen for proanthocyanidins in 88 different kinds of foods. thirty-nine foods were found to contain proanthocyanidins. these foods include 19 kinds of fruits, eight cereals/beans, seven nuts, two beverages, two spices, and one vegetable. twenty-five kinds of foods were found to contain both oligomeric (dp </= 10) and polymeric proanthocyanidins (dp > 10), and the other 14 foods contained only oligomers. procyanidins with b-type linkages were detec ...200314640607
contribution of individual polyphenolics to total antioxidant capacity of plums.to study the effect of polyphenolics on antioxidant capacities of plums, the amounts of total phenolics, total flavonoids and individual phenolic compounds, and vitamin c equivalent antioxidant capacity (vceac) of eleven plum cultivars was determined. there was a good linear relationship between the amount of total phenolics and total antioxidant capacity (r2 = 0.9887). the amount of total flavonoids and total antioxidant capacity also showed a good correlation (r2 = 0.9653). although the summat ...200314640564
the complete nucleotide sequence of plum pox virus isolates from sweet (ppv-swc) and sour (ppv-soc) cherry and their taxonomic relationships within the species.plum pox virus (ppv) sweet (swc) and sour (soc) cherry isolates were the first ppv isolates to be recovered from natural infection in sweet and sour cherry plants, respectively. their complete nucleotide sequences have been determined finding a deduced genome organisation typical for ppv species. both genomes are 9795 nucleotides long, excluding the 3' terminal poly(a) tail, and contain an open reading frame of 9432 nt, encoding a polyprotein of 3143 amino acids. the nucleotide and predicted ami ...200314579174
location of independent root-knot nematode resistance genes in plum and peach.prunus species express different ranges and levels of resistance to the root-knot nematodes (rkn) meloidogyne spp. in myrobalan plum ( prunus cerasifera), the dominant ma gene confers a high-level and wide-spectrum resistance to the predominant rkn, meloidogyne arenaria, meloidogyne incognita, meloidogyne javanica and the isolate meloidogyne sp. florida which overcomes the resistance of the amygdalus sources. in japanese plum ( prunus salicina), a similar wide-spectrum dominant resistance gene, ...200414569426
substances which inhibit ice nucleation: a review.there are a number of substances described in the published literature which inhibit ice nucleation. certain bacterial strains, mostly found among the nonfluorescent pseudomonade species, release material into the growth medium which reduces the nucleation temperature of water droplets to below that of distilled water. extracts from the seeds of food crops including apricot, peach and plum can reduce the nucleation temperature of water droplets and dispersions of silver iodide. antifreeze glycop ...200314566386
quantification of polyphenolics and their antioxidant capacity in fresh plums.total phenolics, total flavonoids, and antioxidant capacity of 11 cultivars of fresh plums were determined using spectrophotometric methods. identification and quantification of individual polyphenolics were performed using reversed-phase high-performance liquid chromatography equipped with a diode array detector. the total phenolic contents of various cultivars widely varied from 125.0 to 372.6 mg/100 g expressed as gallic acid equivalents. the level of total flavonoids in fresh plums ranged be ...200314558771
electro-acupuncture combined with plum-blossom needle tapping for treatment of supraorbital neuritis--a clinical observation of 59 cases. 200314535183
defined medium for growth of foot-and-mouth disease virus.pledger, richard a. (plum island animal disease laboratory, greenport, n. y.) and jerome polatnick. defined medium for growth of foot-and-mouth disease virus. j. bacteriol. 83:579-583. 1962.-foot-and-mouth disease virus, grown in primary bovine calf-kidney cell layers with a defined medium containing glucose as the only organic substrate, produced virus titers equivalent to those obtained with complex media containing serum and lactalbumin hydrolyzate. mannose was the only other substrate examin ...196214487167
the composition of plum gums. 195914403355
comparison of the prothrombin-proconvertin determinations (owren-aas) with prothrombin-index obtained with the larsen-plum prothrombin time method. 195514396286
the biosynthesis of polysaccharides. incorporation of d-(1-14c)glucose and d-(6-14c)glucose into plum-leaf polysaccharides.1. the utilization of specifically labelled d-glucose in the biosynthesis of plum-leaf polysaccharides has been studied. after these precursors had been metabolized in plum leaves, the polysaccharides were isolated from the leaves, and their monosaccharide constituents isolated and purified. 2. both the specific activities and the distribution of (14)c along the carbon chains of the monosaccharides were determined. significant (14)c activity was found in units of d-galactose, d-glucose, d-xylose ...196514342252
influence of tissue culture passage on virulence of foot-and-mouth disease virus for mother mice.campbell, charles h. (plum island animal disease laboratory, greenport, n.y.). influence of tissue culture passage on virulence of foot-and-mouth disease virus for mother mice. j. bacteriol. 86:593-597. 1963.-foot-and-mouth disease virus serially passaged in bovine cell cultures was more lethal for mother mice than the parent virus from infected steers. in tests with virus from several pools of bovine tongue tissue, only 0 to 20% of the mice died. serial passage of such virus in primary calf-kid ...196314066443
the biosynthesis and metabolism of polyols. sorbitol (d-glucitol) of plum leaves. 196113861243
the biosynthesis and metabolism of polyols. 2. the metabolism of 14c-labelled d-glucose, d-glucuronic acid and d-glucitol (sorbitol) by plum leaves. 196213861242
suppressor activity of potyviral and cucumoviral infections in potyvirus-induced transgene silencing.the process known as 'recovery' by which virus-infected plants become resistant to the infection is an interesting phenomenon where both rna silencing and virus resistance fully converge. in a previous study, we showed that transgenic nicotiana benthamiana nibv3 plants, transformed with a mutated nib coding sequence from plum pox virus (ppv), showed a delayed, very specific, resistance phenotype, which was induced by the initial infection. this recovery was the consequence of the activation of a ...200313679623
fluorescent labelling reveals spatial separation of potyvirus populations in mixed infected nicotiana benthamiana plants.the distribution of potyviruses in mixed infected nicotiana benthamiana plants was investigated by using green and red fluorescent proteins (gfp, dsred). full-length cdna clones of plum pox virus (ppv-nat-agfps; ppv-nat-red), tobacco vein mottling virus (tvmv-gfp; tvmv-red) and clover yellow vein virus (clyvv-gfp) expressing fluorescent proteins, referred to here as labelled viruses, were used to characterize the distribution of different potyviral populations (e.g. tvmv-gfp/ppv-nat-red), as wel ...200313679622
the metabolism of d-glucuronic acid in plum tissue and its relation to gummosis. 195513269152
[plum-sized para-urethral diverticulum with calculus in a 50 year old woman]. 195413173741
[erosive effect of the hog plum (spondias lutea, l.) on the lower molars of the albino rat]. 195313134736
elimination of ppv and pnrsv through thermotherapy and meristem-tip culture in nectarine.the plum pox virus (ppv) and prunus necrotic ringspot virus (pnrsv) cause serious disease problems in stone-fruit trees. in this work, the possibility of obtaining plant material free from these viruses through thermotherapy and meristem-tip culture from infected nectarine shoots (prunus persica var. nectarina max, cv. 'arm king') was studied. in addition, the detection of these viruses in in vitro cultures and young acclimatized plantlets with double antibody sandwich-enzyme-linked immunosorben ...200312898177
photoelectrochemical solar cell using extract of eugenia jambolana lam as a natural sensitizer.the extract of jambol o (java plum), eugenia jambolana lam, was used as a natural sensitizer of a wide band-gap semiconductor (tio2) in photoelectrochemical solar cells. the natural dye, adsorbed onto the semiconductor surface, absorbs visible light and promotes electron transfer across the dye/semiconductor interface. photogenerated current and voltage as high as 2.3 ma and 711 mv, respectively, were obtained and effective conversion of visible light into electricity was achieved. the use of a ...200312894301
evaluation of the radioprotective effect of the leaf extract of syzygium cumini (jamun) in mice exposed to a lethal dose of gamma-irradiation.the effects of various concentrations (5, 10, 20, 30, 40, 50, 60, and 80 mg/kg body weight (b.wt.) of the leaf extracts of syzygium cumini linn. and eugenia cumini (sce, black plum, jamun, family myrtaceae) on the radiation-induced sickness and mortality in mice exposed to 10 gy gamma-irradiation were studied. the treatment of mice with different doses of sce, consecutively for five days before irradation, delayed the onset of mortality and reduced the symptoms of radiation sickness when compare ...200312866620
odor-baited trap trees: a new approach to monitoring plum curculio (coleoptera: curculionidae).we compared a trap approach with a trap-tree approach to determine the need and timing of insecticide applications against overwintered adult plum curculios, conotrachelus nenuphar (herbst.), in commercial apple orchards in massachusetts in 2002. all traps and trap trees were baited with benzaldehyde (attractive fruit odor) plus grandisoic acid (attractive pheromone). sticky clear plexiglas panel traps placed at orchard borders, designed to intercept adults immigrating from border areas by fligh ...200312852623
an apricot (prunus armeniaca l.) f2 progeny linkage map based on ssr and aflp markers, mapping plum pox virus resistance and self-incompatibility traits.a genetic linkage map of apricot ( prunus armeniaca l.) was constructed using aflp and ssr markers. the map is based on an f(2) population (76 individuals) derived from self-pollination of an f(1) individual ('lito') originated from a cross between 'stark early orange' and 'tyrinthos'. this family, designated as 'lito' x 'lito', segregated for two important agronomical traits: plum pox virus resistance (ppv) and self-incompatibility. a total of 211 markers (180 aflps, 29 ssrs and two agronomic t ...200312845439
effect of temperature on plum pox virus infection.one of the key factors of progress of an epidemic is the duration of virus availability for a vector in plants, which could be influenced by temperature. using five epidemiologically different isolates of plum pox virus (ppv) we studied the effect of temperature on the virus infectivity, intensity of disease symptoms and virus accumulation in nicotiana benthamiana plants as determined by a double-antibody sandwich-enzyme-linked immunosorbent assay (das-elisa). no differences in infectivity and i ...200312828345
early antibiotic selection and efficient rooting and acclimatization improve the production of transgenic plum plants (prunus domestica l.).we describe here an improved system for routinely developing transgenic plum plants (prunus domestica l.) through the use of agrobacterium tumefaciens. the production of non-transformed "escapes" has been virtually eliminated, and rates of plant establishment in the greenhouse have been dramatically improved. the system is based on the regeneration of shoots from hypocotyls extracted from mature seed. the shoot regeneration medium is murashige and skoog (ms) salts and vitamins supplemented with ...200312827433
expression of self-complementary hairpin rna under the control of the rolc promoter confers systemic disease resistance to plum pox virus without preventing local infection.homology-dependent selective degradation of rna, or post-transcriptional gene silencing (ptgs), is involved in several biological phenomena, including adaptative defense mechanisms against plant viruses. small interfering rnas mediate the selective degradation of target rna by guiding a multicomponent rnase. expression of self-complementary hairpin rnas within two complementary regions separated by an intron elicits ptgs with high efficiency. plum pox virus (ppv) is the etiological agent of shar ...200312823862
'candidatus phytoplasma phoenicium' sp. nov., a novel phytoplasma associated with an emerging lethal disease of almond trees in lebanon and iran.almonds (prunus amygdalus) represent an important crop in most mediterranean countries. a new and devastating disease of almond trees in lebanon was recently reported, characterized by the development of severe witches'-brooms on which no flowers or fruits developed, and leading to tree death within a few years. a phytoplasma was detected in diseased trees by pcr amplification of rrna operon sequences, and rflp patterns of amplified dna indicated that the phytoplasma belonged to the pigeon pea w ...200312807209
identification of odors from overripe mango that attract vinegar flies, drosophila melanogaster.bioassays with a variety of overripe fruits, including mango, plum, pear, and grape, and their extracts showed that odors from overripe mango were most attractive to adult vinegar flies, drosophila melanogaster. combined gas chromatography-electroantennographic detection (gc-ead) analyses of solid-phase microextraction (spme) and tenax extracts of overripe mango odors showed that several volatile compounds, including ethanol, acetic acid, amyl acetate, 2-phenylethanol, and phenylethyl acetate el ...200312775150
from plums to prunes: influence of drying parameters on polyphenols and antioxidant activity.prunes, which are industrially obtained by dehydrating fresh plums at 85-90 degrees c for 18 h, contain higher levels of phenolic compounds than most other fruits. prune phenolics have shown beneficial effects on human health. reports are available in the literature on ascorbic acid, phenol composition, and antioxidant activity of fresh plums and prunes, but there is a lack of publications on the influence of drying parameters on the phenolic compounds and antioxidant activity. a study was carri ...200312769544
climatic factors governing plant phenological phases along a norwegian fjord.in the present project, the time of leaf budding and flowering, and partly also of fruit ripening, was studied over 3 years in different cultivated and native plants on a gradient along a western norwegian fjord about 300 km long, from oceanic to relatively continental regions. in the plants investigated, flowering of the red currant was most strongly favoured by oceanic conditions in the outermost part of the fjord. on the other hand, flowering of the apple was earliest in the middle district, ...200312750970
determination of antioxidant properties of aromatic herbs, olives and fresh fruit using an enzymatic sensor.the aim was to experimentally evaluate the antioxidant capacity of different fresh aromatic herbs (field balm, marjoram, parsley, rosemary, sage, sweet basil), several varieties of olives from central italy ('carboncello', 'rosciolo', 'olivastro', 'coratello', 'leccino', 'frantoio') and several types of fresh fruit (apple, apricot, banana, cherry, fig, grape, medlar, melon, peach, pear, pineapple, plum, water melon, yellow plum) using a superoxide dismutase (sod) biosensor developed by the prese ...200312733012
using the plum procedure of spss to fit unequal variance and generalized signal detection models.the recent addition of aprocedure in spss for the analysis of ordinal regression models offers a simple means for researchers to fit the unequal variance normal signal detection model and other extended signal detection models. the present article shows how to implement the analysis and how to interpret the spss output. examples of fitting the unequal variance normal model and other generalized signal detection models are given. the approach offers a convenient means for applying signal detectio ...200312723779
quantitative evaluation of antioxidant components in prunes (prunus domestica l.).prunes are known to show high antioxidant activity on the basis of the oxygen radical absorbance capacity (orac), and their major antioxidant components are caffeoylquinic acid isomers. the aim of this study is to prove the contribution of caffeoylquinic acid isomers to the orac of prunes, and to investigate the existence of other antioxidant components. caffeoylquinic acid isomers in ethanol (etoh) extracts of prunes were quantified by hplc analysis, and the degree of contribution of these isom ...200312590502
development of microsatellite markers in peach [ prunus persica (l.) batsch] and their use in genetic diversity analysis in peach and sweet cherry ( prunus avium l.).we report the sequence of 41 primer pairs of microsatellites from a ct-enriched genomic library of the peach cultivar 'merrill o'henry'. ten microsatellite-containing clones had sequences similar to plant coding sequences in databases and could be used as markers for known functions. for microsatellites segregating at least in one of the two prunus f(2) progenies analyzed, it was possible to demonstrate mendelian inheritance. microsatellite polymorphism was evaluated in 27 peach and 21 sweet che ...200212582570
genetic diversity in apricot revealed by aflp markers: species and cultivar comparisons.the genetic diversity of apricot ( prunus armeniaca; 2n = 16) was studied using aflp markers. forty seven apricot cultivars were selected from the following geographic regions: europe, north america, north africa, turkey, iran and china. five ecori- msei aflp primer combinations revealed 416 legible bands, of which 379 were polymorphic markers. a similarity matrix was prepared using the simple matching coefficient of similarity. a upgma dendrogram demonstrated a gradient of decreasing genetic di ...200212582532
genetic linkage maps of two apricot cultivars ( prunus armeniaca l.), and mapping of ppv (sharka) resistance.genetic linkage maps for two apricot cultivars have been constructed using aflp, rapd, rflp and ssr markers in 81 f1 individuals from the cross 'goldrich' x 'valenciano'. this family segregated for resistance to 'plum pox virus' (ppv), the most-important virus affecting prunus species. of the 160 rapd arbitrary primers screened a total of 44 were selected. sixty one polymorphic rapd markers were scored on the mapping population: 30 heterozygous in 'goldrich', 19 heterozygous in 'valenciano', seg ...200212582518
generation and characterisation of functional recombinant antibody fragments against rna replicase nib from plum pox virus.a monoclonal antibody (mab 2a) able to react against the rna replicase nib from plum pox virus (ppv) was obtained and used for generating a specific scfv fragment. the vh and vl coding sequences were cloned and expressed as a fusion scfv protein to alkaline phosphatase. this fusion protein was able to recognise viral nib in both western and tissue-print elisa blots. the affinity and specificity of scfv2a for nib was similar to that of the parental mab and the region yleafy from ppv-nib was ident ...200312535657
molecular studies of the synergistic interactions between plum pox virus hc-pro protein and potato virus x.helper component proteinase (hc-pro) is a multifunctional viral protein involved in vection and movement of potyvirus; suppression of host post-transcriptional gene silencing reaction; and synergism of potyvirus with other viruses, notably potexvirus. when the hc-pro of plum pox potyvirus (ppv) was transiently expressed in n. benthamiana and n. clevelandii plants via potato virus x (pvx) vector, a highly synergistic response of leaf necrosis or plant death, was observed in pvxhc-infected plants. ...200212491099
zantedeschia mosaic virus causing leaf mosaic symptom in calla lily is a new potyvirus.anovel virus, zantedeschia mosaic virus (zamv-kr), causing mosaic and malformation symptoms was isolated from calla lily ( zantedeschia spp.) in korea and its biological and molecular properties were characterized. the virus was distinct from dasheen mosaic virus, an araceae-infecting potyvirus, by serological and sequence analyses. multiple alignments of the cp amino acid sequence between the virus and other potyviruses showed 51.8 to 62.1% identity. phylogenetic analyses of the cp revealed tha ...200212491097
fruit-juice concentrate of asian plum inhibits growth signals of vascular smooth muscle cells induced by angiotensin ii.bainiku-ekisu, the fruit-juice concentrate of the oriental plum (prunus mume) has recently been shown to improve human blood fluidity. we have shown that angiotensin ii (angii) stimulates growth of vascular smooth muscle cells (vsmcs) through epidermal growth factor (egf) receptor transactivation that involves reactive oxygen species (ros) production. to better understanding the possible cardiovascular protective effect of bainiku-ekisu, we have studied whether bainiku-ekisu inhibits angii-induc ...200212467906
identification of immunogenic hot spots within plum pox potyvirus capsid protein for efficient antigen presentation.pepscan analysis has been used to characterize the immunogenic regions of the capsid protein (cp) in virions of plum pox potyvirus (ppv). in addition to the well-known highly immunogenic n- and c-terminal domains of cp, regions within the core domain of the protein have also shown high immunogenicity. moreover, the n terminus of cp is not homogeneously immunogenic, alternatively showing regions frequently recognized by antibodies and others that are not recognized at all. these results have help ...200212438590
general-purpose infusion pumps.general-purpose infusion pumps deliver liquid medications and other solutions to patients through intravenous (i.v.) or epidural routes at specified flows. they are most often used when greater accuracy or higher flows are needed than can be provided by manually adjusted gravity systems. in this evaluation (which updates our february 1997 and april-may 1998 studies), we present our findings for four newly evaluated pumps from three suppliers: the abbott plum a+, the alaris medley medication safe ...200212436838
the production of a genus-specific recombinant antibody (scfv) using a recombinant potyvirus protease.a single chain variable fragment antibody (scfv; anti-nia scfv102) was selected from a synthetic human antibody library by using a nia protease of plum pox virus (ppv) as an antigen, which was expressed in bacteria. the nia protease forms the nuclear inclusion body a and acts as the major protease in the cleavage of the viral polyprotein into functional proteins. the nia protein was detected with anti-nia scfv102 after expression in escherichia coli cells as well as from ppv-infected nicotiana b ...200212393153
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