Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
stylet morphometrics and citrus leaf vein structure in relation to feeding behavior of the asian citrus psyllid diaphorina citri, vector of citrus huanglongbing bacterium.the asian citrus psyllid (acp), diaphorina citri (hemiptera: psyllidae), is the primary vector of the phloem-limited bacterium candidatus liberibacter asiaticus (las) associated with huanglongbing (hlb, citrus greening), considered the world's most serious disease of citrus. stylet morphometrics of acp nymphs and adults were studied in relation to citrus vein structure and to their putative (histologically verified) feeding sites on valencia orange leaves. acp nymphs preferred to settle and feed ...201323555830
fumigant toxicity of citrus sinensis essential oil on musca domestica l. adults in the absence and presence of a p450 inhibitor.essential oils (eos) are potential tools for controlling musca domestica l. in a fumigant assay, m. domestica adults treated with citrus sinensis eo (lc50=3.9mg/dm(3)), with (4r)(+)-limonene (95.1%) being its main component, died within 15min or less. the terpenes absorbed by the flies and their metabolites, analyzed using spme fiber, were (4r)(+)-limonene (lc50=6.2mg/dm(3)), α-pinene (lc50=11.5mg/dm(3)), β-pinene (lc50=6.4mg/dm(3)), and two new components, carveol (lc50=1122mg/dm(3)) and carvon ...201323545130
computational predictions provide insights into the biology of tal effector target sites.transcription activator-like (tal) effectors are injected into host plant cells by xanthomonas bacteria to function as transcriptional activators for the benefit of the pathogen. the dna binding domain of tal effectors is composed of conserved amino acid repeat structures containing repeat-variable diresidues (rvds) that determine dna binding specificity. in this paper, we present talgetter, a new approach for predicting tal effector target sites based on a statistical model. in contrast to prev ...201323526890
transcriptional profiling of canker-resistant transgenic sweet orange (citrus sinensis osbeck) constitutively overexpressing a spermidine synthase gene.citrus canker disease caused by xanthomonas citri subsp. citri (xcc) is one of the most devastating diseases affecting the citrus industry worldwide. in our previous study, the canker-resistant transgenic sweet orange (citrus sinensis osbeck) plants were produced via constitutively overexpressing a spermidine synthase. to unravel the molecular mechanisms underlying xcc resistance of the transgenic plants, in the present study global transcriptional profiling was compared between untransformed li ...201223509803
metabolic responses to iron deficiency in roots of carrizo citrange [citrus sinensis (l.) osbeck. x poncirus trifoliata (l.) raf].the effects of iron (fe) deficiency on the low-molecular-weight organic acid (lmwoa) metabolism have been investigated in carrizo citrange (cc) [citrus sinensis (l.) osb. × poncirus trifoliata (l.) raf.] roots. major lmwoas found in roots, xylem sap and root exudates were citrate and malate and their concentrations increased with fe deficiency. the activities of several enzymes involved in the lmwoa metabolism were also assessed in roots. in the cytosolic fraction, the activities of malate dehyd ...201323462311
characterization of citrus sinensis transcription factors closely associated with the non-host response to xanthomonas campestris pv. vesicatoria.plants, when exposed to certain pathogens, may display a form of genotype-independent resistance, known as non-host response. in this study, the response of citrus sinensis (sweet orange) leaves to xanthomonas campestris pv. vesicatoria (xcv), a pepper and tomato pathogenic bacterium, was analyzed through biochemical assays and cdna microarray hybridization and compared with asiatic citrus canker infection caused by xanthomonas citri subsp. citri. citrus leaves exposed to the non-host bacterium ...201323453188
modification of carotenoid levels by abscission agents and expression of carotenoid biosynthetic genes in 'valencia' sweet orange.the effect of 5-chloro-3-methyl-4-nitro-1h-pyrazole (cmnp) and ethephon on peel color, flavedo carotenoid gene expression, and carotenoid accumulation was investigated in mature 'valencia' orange ( citrus sinensis l. osbeck) fruit flavedo at three maturation stages. abscission agent application altered peel color. cmnp was more effective than ethephon in promoting green-to-red (a) and blue-to-yellow (b) color at the middle and late maturation stages and total carotenoid changes at all maturation ...201323451824
new excised-leaf assay method to test inoculativity of asian citrus psyllid (hemiptera: psyllidae) with candidatus liberibacter asiaticus associated with citrus huanglongbing disease.the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: psyllidae), is the primary vector of candidatus liberibacter asiaticus (las) associated with huanglongbing, or citrus greening, the most devastating citrus (citrus spp.) disease worldwide. here, we developed a new "excised-leaf assay" that can speed up las-inoculativity tests on asian citrus psyllid from the current 3-12 mo (when using whole citrus seedlings for inoculation) to only 2-3 wk. young adults of asian citrus psyllid that ...201323448011
quantification of hesperidin in citrus-based foods using a fungal diglycosidase.a simple enzymatic-spectrophotometric method for hesperidin quantification was developed by means of a specific fungal enzyme. the method utilises the diglycosidase α-rhamnosyl-β-glucosidase (ec 3.2.1.168) to quantitatively hydrolyse hesperidin to hesperetin, and the last is measured by its intrinsic absorbance in the uv range at 323 nm. the application of this method to quantify hesperidin in orange (citrus sinensis) juices was shown to be reliable in comparison with the standard method for fla ...201223442693
variations in type iii effector repertoires, pathological phenotypes and host range of xanthomonas citri pv. citri pathotypes.the mechanisms determining the host range of xanthomonas are still undeciphered, despite much interest in their potential roles in the evolution and emergence of plant pathogenic bacteria. xanthomonas citri pv. citri (xci) is an interesting model of host specialization because of its pathogenic variants: pathotype a strains infect a wide range of rutaceous species, whereas pathotype a*/a(w) strains have a host range restricted to mexican lime (citrus aurantifolia) and alemow (citrus macrophylla) ...201323437976
effect of the use of a neutralizing step after antimicrobial application on microbial counts during challenge studies for orange disinfection.the effects of using a neutralizer after applying antimicrobial treatments and the effect of time lapse between treatment application and subsequent recovery and enumeration of escherichia coli o157:h7 and salmonella were investigated in valencia oranges. inoculated oranges surfaces were washed with distilled water for 15 s and then sprayed with a solution containing 200 mg/liter sodium hypochlorite (ph 6.5) for 15 s; they were then dipped in l-lactic acid (2.0% at 55°c) for 1 min or in distille ...201323433383
[analysis of chlorophyll in gannan navel orange with algorithm of ga and spa based on hyperspectral].the hyperspectral effective information of gannan navel orange was extracted by genetic algorithm (ga) and successive projections algorithm (spa) for partial least squares (pls) model. average spectral was extracted from region of interest (rod) of hyperspectral images after preprocessing. ga and spa were used to select 27 and 8 characteristic wavelengths for chlorophyll model with pls. the correlation coefficients and rms error of ga-pls were 0.80 and 2.45, and the correlation coefficients and ...201223427571
[determination of soluble solids content in navel oranges by vis/nir diffuse transmission spectra combined with cars method].soluble solids content (ssc) is one of important internal quality index for navel oranges. in the present study, visible/near infrared (vis/nir) diffuse transmission spectra of navel oranges were acquired using a qualityspec spectrometer in the wavelength range of 350-1 000 nm, and cars (competitive adaptive reweighted sampling) was used to select important variables related with ssc of navel oranges from spectra data, then was compared with other variable selection methods such as uninformative ...201223427541
draft genome sequence of fructophilic lactobacillus florum.herein we report the first genome sequence for lactobacillus florum. l. florum 2f was isolated from valencia orange leaves and is fructophilic, like other strains of this species. the draft genome of l. florum 2f contains 1,261,842 bp with a g+c content of 41.5% in 46 contigs (≥500 bp).201323405360
a sensitive method for determination of allergenic fragrance terpene hydroperoxides using liquid chromatography coupled with tandem mass spectrometry.different compositions of monoterpenes are utilized for their pleasant scent in cosmetics and perfumes. however, the most commonly used fragrance terpenes easily oxidize upon contact with air, forming strongly skin-sensitizing hydroperoxides. due to their thermolability and low uv absorbance, detection methods for hydroperoxides are scarce. for the first time, a simple and sensitive method using lc/esi-ms/ms was developed to quantitatively determine hydroperoxides from the common fragrance compo ...201323404959
[identification of different citrus sinensis (l.) osbeck trees varieties using fourier transform infrared spectroscopy and hierarchical cluster analysis].researched on diversity of the spring leaf samples of seven different citrus sinensis (l.) osbeck varieties by fourier transform infrared (ftir) spectroscopy technology, the results showed that the fourier transform infrared spectra of seven varieties leaves was composited by the absorption band of cellulose and polysaccharide mainly, the wave number of characteristics absorption peaks were similar at their ftir spectra. however, there were some differences in shape of peaks and relatively absor ...201223387167
[near-infrared spectrum detection result influenced by navel oranges placement position].the present paper studies the near-infrared spectroscopy of soluble solids content in navel orange that was influenced by different placement positions of the navel orange. according to the different angles between incident light and the straight line composed by navel orange stems and pit, the authors chose three different placement positions,vertical (90 degrees), parallel (0 degrees) and random(not including 0 degrees and 90 degrees). the authors acquired the semi-transmission spectrum of the ...201223387166
[determination of parameter range in detecting cu of gannan navel orange by libs setup].in order to improve the precision and sensitivity of laser-induced breakdown spectroscopy device on detecting heavy metal, parameter optimization should be done. after selecting the initial delay time parameter in detecting navel orange experiment of laser-induced breakdown spectroscopy device, a cell range from 1.10 to 1.30 micros of delay time containing the initial parameter was extracted and experiment on it was conducted. there are more than one peak in the curve of element line intensity a ...201223387151
larvicidal activity of citrus limonoids against aedes albopictus larvae.development of insecticide resistance occurred due to the continuous and misuse of synthetic insecticides therefore, the recent study was conducted to explore eco-friendly plant extracts that have some potential to suppress mosquito larval population.201223378967
metabolism in orange fruits is driven by photooxidative stress in the leaves.in plants, stress signals propagate to trigger distant responses and thus stress acclimation in non-exposed organs. we tested here the hypothesis that leaves submitted to photooxidative stress may influence the metabolism of nearby fruits and thus quality criteria. leaves of orange trees (citrus sinensis (l.) osbeck cv. 'navelate') were acclimated to shade for 1 week and then submitted to full (fl) and medium light (ml) conditions. as expected, photoinhibition was detected in leaves of both fl a ...201323330573
evaluation of codon biology in citrus and poncirus trifoliata based on genomic features and frame corrected expressed sequence tags.citrus, as one of the globally important fruit trees, has been an object of interest for understanding genetics and evolutionary process in fruit crops. meta-analyses of 19 citrus species, including 4 globally and economically important citrus sinensis, citrus clementina, citrus reticulata, and 1 citrus relative poncirus trifoliata, were performed. we observed that codons ending with a- or t- at the wobble position were preferred in contrast to c- or g- ending codons, indicating a close associat ...201323315666
phytochemical profile and antioxidant activity of physiological drop of citrus fruits.the phytochemical content and the antioxidant activity (aa) of physiological drop of the main citrus species grown in china were investigated. among the flavonoids, hesperidin was found mostly in mandarin and sweet orange, naringin was found mostly in sour orange, pummelo, grapefruit and a hybrid (gaocheng), narirutin was found in most varieties, neohesperidin was found in gaocheng and huyou, and nobiletin and tangeretin were found in most varieties. hydroxycinnamic acids were the main phenolic ...201323301602
small rna profiling reveals phosphorus deficiency as a contributing factor in symptom expression for citrus huanglongbing disease.huanglongbing (hlb) is a devastating citrus disease that is associated with bacteria of the genus 'candidatus liberibacter' (ca. l.). powerful diagnostic tools and management strategies are desired to control hlb. host small rnas (srna) play a vital role in regulating host responses to pathogen infection and are used as early diagnostic markers for many human diseases, including cancers. to determine whether citrus srnas regulate host responses to hlb, srnas were profiled from citrus sinensis 10 ...201323292880
[determination: of citrus red 2 in navel orange by ultra performance liquid chromatography-tandem mass spectrometry].an ultra performance liquid chromatography-tandem mass spectrometry (uplc-ms/ ms) method was developed for the determination of citrus red 2 (cr2) in navel orange. the sample was extracted with acetonitrile, cleaned-up by an nh2 solid phase extraction cartridge. then, the analyte was separated on a waters acquity uplc beh c18 column (50 mm x 2.1 mm, 1.7 microm) in gradient elution with the mobile phases of water and acetonitrile. the separated compounds were detected with a waters xevo tq ms tan ...201223256388
synergistic combinations of high hydrostatic pressure and essential oils or their constituents and their use in preservation of fruit juices.this work addresses the inactivation achieved with escherichia coli o157:h7 and listeria monocytogenes egd-e by combined processes of high hydrostatic pressure (hhp) and essential oils (eos) or their chemical constituents (ccs). hhp treatments (175-400 mpa for 20 min) were combined with 200 μl/l of each eo (citrus sinensis l., citrus lemon l., citrus reticulata l., thymus algeriensis l., eucalyptus globulus l., rosmarinus officinalis l., mentha pulegium l., juniperus phoenicea l., and cyperus lo ...201323246609
[experimental study on chromium in gannan navel orange by laser-induced breakdown spectroscopy].it is a relatively new task to apply the laser induced breakdown spectroscopy (libs) to fruit samples. to apply libs technique in the field related to analysis of trace heavy metal element in fruits samples, we have done primary experiments using gannan navel orange samples. the authors put the samples into different concentration gradient k2cr2o7 solution and left it for 30 hours, and then we did the libs experiment, discriminated characteristic spectra of chromium element and recorded the peak ...201223240437
[effectiveness of antimicrobial formulations for acne based on orange (citrus sinensis) and sweet basil (ocimum basilicum l) essential oils].currently, the antimicrobial resistance has developed in bacterial strains involved in the development of acne. therefore, alternatives to antibiotic treatment have become necessary.201623235794
green synthesis of gold nanoparticles using citrus fruits (citrus limon, citrus reticulata and citrus sinensis) aqueous extract and its characterization.this study reports the biological synthesis of gold nanoparticles by the reduction of haucl(4) by using citrus fruits (citrus limon, citrus reticulata and citrus sinensis) juice extract as the reducing and stabilizing agent. a various shape and size of gold nanoparticles were formed when the ratio of the reactants were altered with respect to 1.0mm chloroauric acid solution. the gold nanoparticles obtained were characterized by uv-visible spectra, transmission electron microscopy (tem) and x-ray ...201323211617
effects of fruit juices of citrus sinensis l. and citrus limon l. on experimental hypercholesterolemia in the rat.in citrus juices there are various substances of different chemical nature, some of which possess already-known hypocholesterolemic properties. therefore it has seemed interesting to evaluate the effects of a prolonged administration of lyophilized juices of citrus sinensis l. osbeck and citrus limon l. burm fil. in diet-induced rat hypercholesterolemia. lyophilized citrus juices were administered for 15 days (by gavage, at the dose of 5g/kg in aqueous veicle in a volume of 0.5 ml/100 g body wei ...199623194620
the draft genome of sweet orange (citrus sinensis).oranges are an important nutritional source for human health and have immense economic value. here we present a comprehensive analysis of the draft genome of sweet orange (citrus sinensis). the assembled sequence covers 87.3% of the estimated orange genome, which is relatively compact, as 20% is composed of repetitive elements. we predicted 29,445 protein-coding genes, half of which are in the heterozygous state. with additional sequencing of two more citrus species and comparative analyses of s ...201323179022
the genetic diversity of citrus dwarfing viroid populations is mainly dependent on the infected host species.as with viruses, viroids infect their hosts as polymorphic populations of variants. identifying possible sources of genetic variability is significant in the case of the species citrus dwarfing viroid (cdvd) which has been proposed as a dwarfing agent for high-density citrus plantings. here, a natural cdvd isolate (cmc) was used as an inoculum source for long-term (25 years) and short-term (1 year) bioassays in different citrus host species. characterization of progenies indicated that the genet ...201323152366
a reference genetic map of c. clementina hort. ex tan.; citrus evolution inferences from comparative mapping.most modern citrus cultivars have an interspecific origin. as a foundational step towards deciphering the interspecific genome structures, a reference whole genome sequence was produced by the international citrus genome consortium from a haploid derived from clementine mandarin. the availability of a saturated genetic map of clementine was identified as an essential prerequisite to assist the whole genome sequence assembly. clementine is believed to be a 'mediterranean' mandarin × sweet orange ...201223126659
identification of flavonoids with trypsin inhibitory activity extracted from orange peel and green tea leaves.orange peel (citrus sinensis) and green tea (camellia sinensis) leaves, rich sources of food flavonoids, were analyzed for their trypsin inhibitory potential. hesperetin, rutin and hesperidin from orange peel, and catechin from green tea leaves, were isolated and their chemical structures were analyzed. all four compounds were evaluated for their trypsin inhibitory potential.201323124490
development and systematic validation of qpcr assays for rapid and reliable differentiation of xylella fastidiosa strains causing citrus variegated chlorosis.the xylem-limited, gram-negative, fastidious plant bacterium xylella fastidiosa is the causal agent of citrus variegated chlorosis (cvc), a destructive disease affecting approximately half of the citrus plantations in the state of são paulo, brazil. the disease was recently found in central america and is threatening the multi-billion u.s. citrus industry. many strains of x. fastidiosa are pathogens or endophytes in various plants growing in the u.s., and some strains cross infect several host p ...201323123161
isolation and biological activities of decanal, linalool, valencene, and octanal from sweet orange oil.product 1 (82.25% valencene), product 2 (73.36% decanal), product 3 (78.12% octanal), and product 4 (90.61% linalool) were isolated from sweet orange oil by combined usage of molecular distillation and column chromatography. the antioxidant activity of sweet orange oil and these products was investigated using 2,2-diphenyl-1-picrylhydrazyl and reducing power assays. in this test, product 1 (82.25% valencene), product 2 (73.36% decanal), and product 4 (90.61% linalool) had antioxidant activity, b ...201223106968
the genome sequence of an isolate of indian citrus ringspot virus infecting the sweet orange in india.whole-genome sequencing of an isolate of mandarivirus infecting the sweet orange [citrus sinensis (l) blanco] in the western part of india (pune) was done. the single-stranded positive-sense rna genome of indian citrus ringspot virus (icrsv) pune has 7,560 nucleotides (nt), excluding a poly(a) tail, comprised of 27.98% (2,115 nt) a, 32.12% (2,428 nt) c, 19.68% (1,488 nt) g, and 20.22% (1,529 nt) t residues. the genome, organized into six open reading frames (orfs), shares 97.7% sequence identity ...201223087111
a dark incubation period is important for agrobacterium-mediated transformation of mature internode explants of sweet orange, grapefruit, citron, and a citrange rootstock.citrus has an extended juvenile phase and trees can take 2-20 years to transition to the adult reproductive phase and produce fruit. for citrus variety development this substantially prolongs the time before adult traits, such as fruit yield and quality, can be evaluated. methods to transform tissue from mature citrus trees would shorten the evaluation period via the direct production of adult phase transgenic citrus trees.201223082165
molecular cloning and characterization of two novel nac genes from mikania micrantha (asteraceae).nac proteins, which are plant-specific transcription factors, have been identified to play important roles in plant response to stresses and in plant development. the full-length cdnas that encode 2 putative nac proteins, designated as mmataf1 and mmnap, respectively, were cloned from mikania micrantha by rapid amplification of cdna ends. the full-length cdnas of mmataf1 and mmnap were 1329 and 1072 bp, respectively, and they encoded deduced proteins of 260- and 278-amino acid residues, respecti ...201223079980
tetraploid rangpur lime rootstock increases drought tolerance via enhanced constitutive root abscisic acid production.whole-genome duplication, or polyploidy, is common in many plant species and often leads to better adaptation to adverse environmental condition. however, little is known about the physiological and molecular determinants underlying adaptation. we examined the drought tolerance in diploid (2x) and autotetraploid (4x) clones of rangpur lime (citrus limonia) rootstocks grafted with 2x valencia delta sweet orange (citrus sinensis) scions, named v/2xrl and v/4xrl, respectively. physiological experim ...201323050986
citrus phenylpropanoids and defence against pathogens. part ii: gene expression and metabolite accumulation in the response of fruits to penicillium digitatum infection.the effect of infection of citrus sinensis (var. navelina) fruits with penicillium digitatum was studied at gene expression and metabolite levels. in this study, expression of genes involved in the phenylpropanoid pathway was studied in the flavedo (outer coloured part of the peel) and albedo (inner white part) in response to pathogen infection. results of the time-course experiment showed that maximal expression of 10 out of 17 phenylpropanoid genes analysed occurred at 48h post-inoculation, wh ...201323017425
ceramides as possible nutraceutical compounds: characterization of the ceramides of the moro blood orange ( citrus sinensis ).ceramides are presented as nutraceutical compounds for protection of colon carcinoma and as important cosmetic preparation components, increasing absorption through the skin. therefore, the ceramide (cer) content of moro blood oranges was determined by mass spectrometry. a total of 114 cer species were identified: ∼160 mg in the peels and ∼140 mg in the pulp per kilogram of oranges, expressed as "milligram equivalents of d18:1,17:0 cer". the predominant ceramides contained 4-hydroxy-8-sphingenin ...201222985176
protective effects of sweet orange (citrus sinensis) peel and their bioactive compounds on oxidative stress.protective effects of sweet orange (citrus sinensis) peel and their bioactive compounds on oxidative stress were investigated. according to hplc-dad and hplc-ms/ms analysis, hesperidin (hd), hesperetin (ht), nobiletin (nt), and tangeretin (tt) were present in water extracts of sweet orange peel (wesp). the cytotoxic effect in 0.2mm t-bhp-induced hepg2 cells was inhibited by wesp and their bioactive compounds. the protective effect of wesp and their bioactive compounds in 0.2mm t-bhp-induced hepg ...201222980779
on line characterization of 58 phenolic compounds in citrus fruit juices from spanish cultivars by high-performance liquid chromatography with photodiode-array detection coupled to electrospray ionization triple quadrupole mass spectrometry.polyphenol profile of citrus juices of sweet orange, tangerine, lemon and grapefruit from spanish cultivars was obtained by high-performance liquid chromatography with diode array detection coupled to electrospray ionization and triple quadrupole mass spectrometry. fifty eight phenolic compounds of five different classes were identified in these citrus juices. flavanone: o-dihexoside of naringenin; flavones: apigenin-7-o-rutinoside-4'-o-glucoside, luteolin-7-o-neohesperidoside-4'-o-glucoside, lu ...201222967544
a draft of the genome and four transcriptomes of a medicinal and pesticidal angiosperm azadirachta indica.the azadirachta indica (neem) tree is a source of a wide number of natural products, including the potent biopesticide azadirachtin. in spite of its widespread applications in agriculture and medicine, the molecular aspects of the biosynthesis of neem terpenoids remain largely unexplored. the current report describes the draft genome and four transcriptomes of a. indica and attempts to contextualise the sequence information in terms of its molecular phylogeny, transcript expression and terpenoid ...201222958331
d-limonene rich volatile oil from blood oranges inhibits angiogenesis, metastasis and cell death in human colon cancer cells.to identify the chemical constituents of volatile oil from blood orange (citrus sinensis (l) osbeck) and understand the possible mechanisms of inhibition of colon cancer cell proliferation.201222935404
analysis of full-length sequences of two citrus yellow mosaic badnavirus isolates infecting citrus jambhiri (rough lemon) and citrus sinensis l. osbeck (sweet orange) from a nursery in india.citrus yellow mosaic badna virus (cmbv), a member of the family caulimoviridae, genus badnavirus is the causative agent of mosaic disease among citrus species in southern india. despite its reported prevalence in several citrus species, complete information on clear functional genomics or functional information of full-length genomes from all the cmbv isolates infecting citrus species are not available in publicly accessible databases. cmbv isolates from rough lemon and sweet orange collected fr ...201222926812
citrus psorosis and mirafiori lettuce big-vein ophiovirus coat proteins localize to the cytoplasm and self interact in vivo.citrus psorosis (cpsv) and mirafiori lettuce big-vein virus (milbvv) belong to the family ophioviridae, plant viruses with filamentous nucleocapsids and segmented genomes of negative polarity, causing the worldwide distributed citrus psorosis and lettuce big-vein diseases, respectively. to gain insight into the replication cycle of these viruses, the subcellular localization of the viral coat proteins (cp) was studied. immunoblot analysis of fractionated extracts derived from natural and experim ...201222921760
tal effectors target the c-terminal domain of rna polymerase ii (ctd) by inhibiting the prolyl-isomerase activity of a ctd-associated cyclophilin.transcriptional activator-like (tal) effectors of plant pathogenic bacteria function as transcription factors in plant cells. however, how tal effectors control transcription in the host is presently unknown. previously, we showed that tal effectors of the citrus canker pathogen xanthomonas citri, named pthas, targeted the citrus protein complex comprising the thioredoxin cstdx, ubiquitin-conjugating enzymes csuev/ubc13 and cyclophilin cscyp. here we show that cscyp complements the function of c ...201222911812
a new strategy employed for identification of sweet orange cultivars with rapd markers.we optimized rapd techniques by increasing the length of rapd primers and performing a strict screening of pcr annealing temperature to distinguish 60 sweet orange cultivars from the research institute of pomology at the chinese academy of agricultural sciences. a new approach called cultivar identification diagram (cid) was used to improve the efficiency of rapd markers for cultivar identification. thirteen effective primers were first screened from 54 rapd arbitrary 11-mer primers based on the ...201222911591
evaluation of bioefficacy of three citrus essential oils against the dengue vector aedes albopictus (diptera: culicidae) in correlation to their components enantiomeric distribution.laboratory experiments were conducted to study the bioefficacy against ae. albopictus of three citrus essential oils, derived from peels of citrus sinensis, citrus limon, and citrus paradise and of their components. chiral gas chromatography analysis revealed the dominant occurrence of r-(+)-limonene and (-)-β-pinene in all three essential oils while in the case of lemon oil γ-terpinene, neral, and geranial detected also among other components. the tested citrus essential oils were toxic against ...201222903418
application of orange essential oil as an antistaphylococcal agent in a dressing model.staphylococcus aureus is the pathogen most often and prevalently involved in skin and soft tissue infections. in recent decades outbreaks of methicillin-resistant s. aureus (mrsa) have created major problems for skin therapy, and burn and wound care units. topical antimicrobials are most important component of wound infection therapy. alternative therapies are being sought for treatment of mrsa and one area of interest is the use of essential oils. with the increasing interest in the use and app ...201222894560
a comparison of the ultrastructure and composition of fruits' cuticular wax from the wild-type 'newhall' navel orange (citrus sinensis [l.] osbeck cv. newhall) and its glossy mutant.the altered ultrastructure and composition of cuticular wax from 'glossy newhall' (mt) fruits lead to its glossy phenotype. a novel mutant derived from the wild-type (wt) 'newhall' navel orange (citrus sinensis [l.] osbeck cv. newhall), named 'glossy newhall' (mt), which produced much more glossy fruits that were easily distinguishable from the wt fruits was characterized in this report. the total wax loads of both wt and mt fruits varied considerably during the fruit development. the most abund ...201222892682
the citrus aba signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydration.the abscisic acid (aba) signalling core in plants include the cytosolic aba receptors (pyr/pyl/rcars), the clade-a type 2c protein phosphatases (pp2cas), and the subclass iii snf1-related protein kinases 2 (snrk2s). the aim of this work was to identify these aba perception system components in sweet orange and to determine the influence of endogenous aba on their transcriptional regulation during fruit development and ripening, taking advantage of the comparative analysis between a wild-type and ...201222888124
effects of foliar application with compost tea and filtrate biogas slurry liquid on yield and fruit quality of washington navel orange (citrus sinenesis osbeck) trees.sixteen-year-old navel orange trees at a private orchard located in kafer el-sheikh governorate, egypt, were used in this study. compost tea (ct) and filtrate biogas slurry liquid (flb) were applied at two different concentrations (50% and 100%); control trees were sprayed with water trees treated with ct at 100% were the highest in yield, fruit weight, and vitamin c, whereas the highest percentage of fruit set, fruit number and soluble solid content (ssc), lowest fruit drop, and highest reducin ...201222866578
potential of moringa oleifera root and citrus sinensis fruit rind extracts in the treatment of ulcerative colitis in mice.the plant moringa oleifera lam (moringaceae), commonly known as the drumstick tree, is an indigenous species in india. this species has been of interest to researchers because traditionally its roots are reported in the treatment of ulcerative colitis (uc). traditionally it is reported that citrus sinensis linn (rutaceae) fruit rind when combined with m. oleifera will increase the efficacy of the plant in the treatment of uc.201222849565
effect of sweet orange aroma on experimental anxiety in humans.the objective of this study was to evaluate the potential anxiolytic effect of sweet orange (citrus sinensis) aroma in healthy volunteers submitted to an anxiogenic situation.201222849536
different transcriptional response to xanthomonas citri subsp. citri between kumquat and sweet orange with contrasting canker tolerance.citrus canker disease caused by xanthomonas citri subsp. citri (xcc) is one of the most devastating biotic stresses affecting the citrus industry. meiwa kumquat (fortunella crassifolia) is canker-resistant, while newhall navel orange (citrus sinensis osbeck) is canker-sensitive. to understand the molecular mechanisms underlying the differences in responses to xcc, transcriptomic profiles of these two genotypes following xcc attack were compared by using the affymetrix citrus genome genechip. a t ...201222848606
comparative transcriptional and anatomical analyses of tolerant rough lemon and susceptible sweet orange in response to 'candidatus liberibacter asiaticus' infection.although there are no known sources of genetic resistance, some citrus spp. are reportedly tolerant to huanglongbing (hlb), presumably caused by 'candidatus liberibacter asiaticus'. time-course transcriptional analysis of tolerant rough lemon (citrus jambhiri) and susceptible sweet orange (c. sinensis) in response to 'ca. l. asiaticus' infection showed more genes differentially expressed in hlb-affected rough lemon than sweet orange at early stages but substantially fewer at late time points, po ...201222809274
the influence of citrus rootstocks on the relationship between the mite brevipalpus phoenicis and citrus leprosis disease.leprosis is one of the most serious citrus plant diseases. leprosis-affected plants, especially sweet orange [citrus sinensis (l.) osbeck], which is the most widely cultivated citrus fruit worldwide, show reduced photosynthetic capacity and severe defoliation. the aim was to evaluate the relationship between the brevipalpus phoenicis (geijskes) vector mite and citrus leprosis disease in pera sweet orange plants grafted on different rootstocks. data were analysed using numerical classification an ...201322807324
gus expression in sweet oranges (citrus sinensis l. osbeck) driven by three different phloem-specific promoters.huanglongbing (hlb) is associated with candidatus liberibacter spp., endogenous, sieve tube-restricted bacteria that are transmitted by citrus psyllid insect vectors. transgenic expression in the phloem of specific genes that might affect ca. liberibacter spp. growth and development may be an adequate strategy to improve citrus resistance to hlb. to study specific phloem gene expression in citrus, we developed three different binary vector constructs with expression cassettes bearing the β-glucu ...201222801867
comparison of antifungal activities of vietnamese citrus essential oils.citrus essential oils (eos) are volatile compounds from citrus peels and widely used in perfumes, cosmetics, soaps and aromatherapy. in this study, inhibition of citrus eos extracted from vietnamese orange (citrus sinensis), mandarin (citrus reticulata blanco), pomelo (citrus grandis osbeck) and lime (citrus aurantifolia swingle) on the growth of plant pathogenic fungi, mucor hiemalis, penicillium expansum and fusarium proliferatum was investigated. the eos of the citrus peels were obtained by c ...201322799453
larvicidal, pupicidal, repellent and adulticidal activity of citrus sinensis orange peel extract against anopheles stephensi, aedes aegypti and culex quinquefasciatus (diptera: culicidae).mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikunguniya fever, west nile virus and yellow fever. these diseases produce significant morbidity and mortality in humans and livestock around the world. the present study explored the effects of orange peel ethanol extract of citrus sinensis on larvicidal, pupicidal, repellent and adulticidal activity against anopheles stephensi, aedes aegypti and culex quinquefasciatus. the or ...201222797605
field performance of transgenic citrus trees: assessment of the long-term expression of uida and nptii transgenes and its impact on relevant agronomic and phenotypic characteristics.the future of genetic transformation as a tool for the improvement of fruit trees depends on the development of proper systems for the assessment of unintended effects in field-grown gm lines. in this study, we used eight transgenic lines of two different citrus types (sweet orange and citrange) transformed with the marker genes β-glucuronidase (uida) and neomycin phosphotransferase ii (nptii) as model systems to study for the first time in citrus the long-term stability of transgene expression ...201222794278
expression and functional analysis of two lycopene β-cyclases from citrus fruits.in the present study, two lcyb genes (citlcyb1 and citlcyb2) were isolated from satsuma mandarin (citrus unshiu marc.), valencia orange (citrus sinensis osbeck) and lisbon lemon (citrus limon burm.f.) and their functions were analyzed by the color complementation assay in lycopene-accumulating e. coli cells. the results showed that citlcyb1 and citlcyb2 shared high identity at the amino acid level among the three citrus varieties. the n-terminal region of the two proteins encoded by citlcyb1 and ...201222729824
quality assessment of industrially processed fruit juices available in dhaka city, bangladesh.this study was performed to evaluate the nutritional and microbiological quality of industrially processed packed fruit juices of mango (mangifera indica) and orange (citrus sinensis) from nine different manufacturing companies in dhaka city. the highest quantity of total sugar (17.62%) and reducing sugar (9.99%) was recorded in mango juices while the lowest in orange juices (10.41% and 2.24% respectively) of different companies. in this study, protein contents were comparatively higher in mango ...201022691996
phylogeographic structure, outbreeding depression, and reluctant virgin oviposition in the bean thrips, caliothrips fasciatus (pergande) (thysanoptera: thripidae), in california.bean thrips, caliothrips fasciatus, is native to western north america. once considered a pest of several crops in its native area, its pest status has waned over recent decades. however, due to its habit of aggregating in the navel of navel oranges, bean thrips remains economically important because some countries importing oranges from california have designated it a quarantine pest. despite continued propagule pressure, bean thrips has never established outside north america. we examined gene ...201222676974
a lov protein modulates the physiological attributes of xanthomonas axonopodis pv. citri relevant for host plant colonization.recent studies have demonstrated that an appropriate light environment is required for the establishment of efficient vegetal resistance responses in several plant-pathogen interactions. the photoreceptors implicated in such responses are mainly those belonging to the phytochrome family. data obtained from bacterial genome sequences revealed the presence of photosensory proteins of the bluf (blue light sensing using fad), lov (light, oxygen, voltage) and phytochrome families with no known functi ...201222675525
construction of egfp-labeling system for visualizing the infection process of xanthomonas axonopodis pv. citri in planta.xanthomonas axonopodis pv. citri (xac) is the causal agent of citrus bacterial canker, an economically important disease to world citrus industry. to monitor the infection process of xac in different citrus plants, the enhanced green florescent protein (egfp) visualizing system was constructed to visualize the propagation and localization in planta. first, the wild-type xac was isolated from the diseased leaves of susceptible 'bingtang' sweet orange, and then the isolated xac was labeled with eg ...201222674174
genetic diversity and population differentiation of the causal agent of citrus black spot in brazil.one of the most important diseases that affect sweet orange orchards in brazil is the citrus black spot that is caused by the fungus guignardia citricarpa. this disease causes irreparable losses due to the premature falling of fruit, as well as its severe effects on the epidermis of ripe fruit that renders them unacceptable at the fresh fruit markets. despite the fact that the fungus and the disease are well studied, little is known about the genetic diversity and the structure of the fungi popu ...201222666111
citrus and prunuscopia-like retrotransposons.many of the world's most important citrus cultivars ("washington navel", satsumas, clementines) have arisen through somatic mutation. this phenomenon occurs fairly often in the various species and varieties of the genus.the presence of copia-like retrotransposons has been investigated in fruit trees, especially citrus, by using a pcr assay designed to detect copia-like reverse transcriptase (rt) sequences. amplification products from a genotype of each the following species citrus sinensis, citr ...199922665184
exogenous treatment with salicylic acid attenuates occurrence of citrus canker in susceptible navel orange (citrus sinensis osbeck).citrus canker caused by xanthomonas axonopodis pv. citri (xac) is a devastating bacterial disease threatening the citrus industry. salicylic acid (sa) plays a key role in plant defense response to biotic stress, but information is scarce concerning the application of sa to enhancing xac resistance. in the present research attempts were made to investigate how exogenous application of sa influenced canker disease outbreak in navel orange (citrus sinensis). exogenously applied sa at 0.25 mm signif ...201222658220
use of honey bees (apis mellifera l.) to detect the presence of mediterranean fruit fly (ceratitis capitata wiedemann) larvae in valencia oranges.when fruit deteriorates a characteristic profile of volatile chemicals is produced that is different from that produced by healthy fruits. the identification of such chemicals allows the possibility of monitoring the fruit for early signs of deterioration with biological sensors. the use of honey bees and other insects as biological sensors is well known. this study aimed to identify the volatiles produced by oranges infested with larvae of the mediterranean fruit fly and to test the ability of ...201222653619
changes of peel essential oil composition of four tunisian citrus during fruit maturation.the present work investigates the effect of ripening stage on the chemical composition of essential oil extracted from peel of four citrus: bitter orange (citrus aurantium), lemon (citrus limon), orange maltaise (citrus sinensis), and mandarin (citrus reticulate) and on their antibacterial activity. essential oils yields varied during ripening from 0.46 to 2.70%, where mandarin was found to be the richest. forty volatile compounds were identified. limonene (67.90-90.95%) and 1,8-cineole (tr-14.7 ...201222645427
transcriptional profiling of genes involved in embryogenic, non-embryogenic calluses and somatic embryogenesis of valencia sweet orange by ssh-based microarray.somatic embryogenesis (se) is a most promising technology that is used for in vitro germplasm conservation and genetic improvement via biotechnological approaches in citrus. herein, three suppression subtractive hybridization (ssh) libraries were constructed using calluses of citrus sinensis cv. 'valencia' to explore the molecular mechanisms that underlie the se in citrus. a total of 880 unisequences were identified by microarray screening based on these three ssh libraries. gene ontology analys ...201222622359
identification of differentially expressed genes in a spontaneous altered leaf shape mutant of the navel orange [citrus sinensis (l.) osbeck].most of the economically important citrus cultivars have originated from bud mutations. leaf shape and structure are important factors that impact plant photosynthesis. we found a spontaneous bud mutant exhibiting a narrow leaf phenotype in navel orange [citrus sinensis (l.) osbeck]. to identify and characterize the genes involved in the formation of this trait, we performed suppression subtractive hybridization (ssh) and macroarray analysis. a total of 221 non-redundant differentially expressed ...201222609459
weedy hosts and prevalence of potential leafhopper vectors (hemiptera: cicadellidae) of a phytoplasma (16srix group) associated with huanglongbing symptoms in citrus groves.huanglongbing (hlb) is a severe citrus (citrus spp.) disease associated with the bacteria genus candidatus liberibacter, detected in brazil in 2004. another bacterium was found in association with hlb symptoms and characterized as a phytoplasma belonging to the 16srix group. the objectives of this study were to identify potential leafhopper vectors of the hlb-associated phytoplasma and their host plants. leafhoppers were sampled every other week for 12 mo with sticky yellow cards placed at two h ...201222606800
validation and characterization of citrus sinensis micrornas and their target genes.micrornas play vital role in plant growth and development by changeable expression of their target genes with most plant micrornas having perfect or near-perfect complementarities with their target genes but mirnas in citrus sinensis (csi-mirnas) and their function have not been widely studied.201222583737
inhibition of beef isolates of e. coli o157:h7 by orange oil at various temperatures.plant essential oils have previously been shown to exhibit antimicrobial activities against various microorganisms. in this study, cold pressed terpeneless valencia orange oil (cptvo) was examined at various temperatures (37, 10, and 4 °c) to determine its antimicrobial activity against 3 strains of e. coli o157:h7 recovered from beef products. a micro broth dilution method using 96 well microtiter plates was used with trypticase soy broth with 0.15% agar and 2,3,5 tetrazolium chloride as a grow ...201222583064
carotenoid accumulation in postharvest "cara cara" navel orange (citrus sinensis osbeck) fruits stored at different temperatures was transcriptionally regulated in a tissue-dependent manner.the main objective of this work was to investigate the effect of storage temperature (4 and 20 °c) on carotenoid accumulation and on the expression levels of seven carotenoid biosynthetic genes (psy, pds, zds, lcyb, lcye, hyb and zep) in postharvest 'cara cara' navel orange (c. sinensis osbeck) fruits. storage at 20 °c rapidly increased the carotenoid content in the peel, whereas the content remained unchanged in the pulp before 35 days of storage. by contrast, storage at 4 °c maintained the car ...201222562781
in vitro effects of citrus oils against mycobacterium tuberculosis and non-tuberculous mycobacteria of clinical importance.we evaluated the in vitro activity of citrus oils against mycobacterium tuberculosis and other non-tuberculous mycobacterium species. citrus essential oils were tested against a variety of mycobacterium species and strains using the bactec radiometric growth system. cold pressed terpeneless valencia oil (cpt) was further tested using the wayne model of in vitro latency. exposure of m. tuberculosis and m. bovis bcg to 0.025 % cold pressed terpeneless valencia orange oil (cpt) resulted in a 3-log ...201222560037
the effects of herbal essential oils on the oviposition-deterrent and ovicidal activities of aedes aegypti (linn.), anopheles dirus (peyton and harrison) and culex quinquefasciatus (say).the effect of oviposition-deterrent and ovicidal of seven essential oils were evaluated towards three mosquito vectors, aedes aegypti, anopheles dirus and culex quinquefasciatus. the oviposition activity index (oai) values of six essential oils namely cananga odorata, cymbopogon citratus, cymbopogon nardus, eucalyptus citriodora, ocimum basilicum and syzygium aromaticum indicated that there were more deterrent than the control whereas citrus sinensis oil acted as oviposition attractant. at highe ...201222543614
structure-activity relationship of citrus polymethoxylated flavones and their inhibitory effects on aspergillus niger.citrus peels are rich in polymethoxylated flavones (pmfs) and are potential sources of natural preservatives. six pmfs extracts, isolated and purified from the peels of three mandarins (citrus reticulata) and three sweet oranges (citrus sinensis), were identified and quantitated. their inhibitory effects on aspergillus niger were evaluated using a microbroth dilution assay. the red tangerine variety exhibited the greatest antifungal activity (mic = 0.2 mg/ml), while jincheng showed the lowest ac ...201222500738
[application of hyperspectral fluorescence image technology in detection of early rotten oranges].rottenness is most prevalent and devastating disease that threats citrus fruit. automatic detection of early rottenness can enhance the competitiveness and profitability of the citrus industry. however, there is no efficient automatic detection technology at this time that could detect this disease. the navel orange was selected as research objective. hyperspectral fluorescence imaging was used to detect early rottenness in orange. optimum index factor (oif) method was applied to identify the op ...201222497146
generation, functional analysis and utility of citrus grandis est from a flower-derived cdna library.pummelo (citrus grandis) is one of the most important species found in the genus citrus and one of the ancestors of sweet oranges. we used flower buds at different developmental stages to construct the first cdna library for this species. a total of 3,758 est sequences were generated from the cdna library and clustered into 2,228 unigenes, comprising 451 contigs and 1,777 singletons. among these unigene sequences, 1,266 have significant homology to the non-redundant protein database, from which ...201222477149
an integrative analysis of transcriptome and proteome provides new insights into carotenoid biosynthesis and regulation in sweet orange fruits.an integrative analysis of transcriptome and proteome was performed to identify differential genes/proteins of a red-flesh sweet orange cara cara in comparison with a common cultivar newhall at ripening stages. at the transcript level, gene expression was measured with massively parallel signature sequencing (mpss), and 629 genes of these two sweet orange cultivars differed by two fold or more (fdr<0.001). at the protein level, a combination of 2de and maldi-tof-tof ms identified 48 protein spot ...201222472342
mistletoes and mutant albino shoots on woody plants as mineral nutrient traps.potassium, sulphur and zinc contents of mistletoe leaves are generally higher than in their hosts. this is attributed to the fact that chemical elements which are cycled between xylem and phloem in the process of phloem loading of sugars are trapped in the mistletoe, because these parasites do not feed their hosts. here it is hypothesized that mutant albino shoots on otherwise green plants should behave similarly, because they lack photosynthesis and thus cannot recycle elements involved in suga ...201222442343
assessment of the genetic diversity of the tunisian citrus rootstock germplasm.citrus represents a substantial income for farmers in the mediterranean basin. however, the mediterranean citrus industry faces increasing biotic and abiotic constraints. therefore the breeding and selection of new rootstocks are now of the utmost importance. in tunisia, in addition to sour orange, the most widespread traditional rootstock of the mediterranean area, other citrus rootstocks and well adapted to local environmental conditions, are traditionally used and should be important genetic ...201222429788
retrotransposons control fruit-specific, cold-dependent accumulation of anthocyanins in blood oranges.traditionally, sicilian blood oranges (citrus sinensis) have been associated with cardiovascular health, and consumption has been shown to prevent obesity in mice fed a high-fat diet. despite increasing consumer interest in these health-promoting attributes, production of blood oranges remains unreliable due largely to a dependency on cold for full color formation. we show that sicilian blood orange arose by insertion of a copia-like retrotransposon adjacent to a gene encoding ruby, a myb transc ...201222427337
chemometric characterization of fruit juices from spanish cultivars according to their phenolic compound contents: i. citrus fruits.the data set composed by phenolic compound profiles of 83 citrus juices (determined by hplc-dad-ms/ms) was evaluated by chemometrics to differentiate them according to citrus species (sweet orange, tangerine, lemon, and grapefruit). cluster analysis (ca) and principal component analysis (pca) showed natural sample grouping among citrus species and even the citrus subclass. most of the information contained in the full data set can be captured if only 15 phenolic compounds (concentration ≥10 mg/l ...201222423971
activity of citrus essential oils against escherichia coli o157:h7 and salmonella spp. and effects on beef subprimal cuts under refrigeration.escherichia coli o157:h7 and salmonella spp. are bacterial pathogens often associated with beef, and cause many cases of foodborne illness each year in the united states. during beef slaughter and processing, these bacteria may spread from the hide or intestines to the carcass. the objective of this research was to investigate the use of naturally occurring compounds citrus essential oils (ceos) extracted from orange peel to reduce or eliminate these pathogens at the chilling stage of processing ...201122417514
effects of cinnamon leaf, oregano and sweet orange essential oils on fermentation and aerobic stability of barley silage.silage additives are marketed with the primary aim of improving the fermentation and/or aerobic stability of silage. the objective of this study was to evaluate the impact of three different essential oils (eos; cinnamon leaf (cin), oregano (ore) and sweet orange (so)) on the fermentation characteristics and stability of barley silage. chopped whole-plant barley (hordem vulgare l.) forage was ensiled either untreated (0 mg kg⁻¹ dry matter (dm)) or treated with cin, ore or so (37.5, 75 and 120 mg ...201222413147
gene expression in citrus sinensis fruit tissues harvested from huanglongbing-infected trees: comparison with girdled fruit.distribution of viable candidatus liberibacter asiaticus (calas) in sweet orange fruit and leaves ('hamlin' and 'valencia') and transcriptomic changes associated with huanglongbing (hlb) infection in fruit tissues are reported. viable calas was present in most fruit tissues tested in hlb trees, with the highest titre detected in vascular tissue near the calyx abscission zone. transcriptomic changes associated with hlb infection were analysed in flavedo (ff), vascular tissue (vt), and juice vesic ...201222407645
inhibition of acetylcholinesterase activity and fe2+-induced lipid peroxidation in rat brain in vitro by some citrus fruit juices.this study sought to characterize the effects of some citrus fruit juices (shaddock [citrus maxima], grapefruit [citrus paradisii], lemon [citrus limoni], orange [citrus sinensis], and tangerine [citrus reticulata]) on acetylcholinesterase activity in vitro. the total phenolic content, radical scavenging abilities, and inhibition of fe(2+)-induced malondialdehyde (mda) production in rats brain homogenate in vitro were also assessed. orange had significantly (p<.05) higher phenolic content than t ...201222400910
enhanced inhibition of listeria monocytogenes by a combination of cold pressed terpeneless valencia orange oil and antibiotics.this study was designed to evaluate the ability of cold pressed terpeneless valencia orange oil (cptvo) to enhance the effectiveness of antibiotics against 10 strains of listeria monocytogenes. disc diffusion assays were performed to determine the effects of cptvo and two antibiotics with different mechanisms of action (i.e., penicillin and chloramphenicol) individually and in combination with cptvo. cptvo alone produced zones ranging from 16.5 to 19.9 mm. penicillin at 2 or 10 units produced zo ...201222394025
antimicrobial effect and mode of action of terpeneless cold-pressed valencia orange essential oil on methicillin-resistant staphylococcus aureus.the objectives of this study were to evaluate the antistaphylococcal effect and elucidate the mechanism of action of orange essential oil against antibiotic-resistant staphylococcus aureus strains.201222372962
infrared spectroscopy: a potential tool in huanglongbing and citrus variegated chlorosis diagnosis.huanglongbing (hlb) and citrus variegated chlorosis (cvc) are serious threats to citrus production and have caused considerable economic losses worldwide, especially in brazil, which is one of the biggest citrus producers in the world. neither disease has a cure nor an efficient means of control. they are also generally confused with each other in the field since they share similar initial symptoms, e.g., yellowing blotchy leaves. the most efficient tool for detecting these diseases is by polyme ...201222365672
volatile constituents of wild citrus mangshanyegan (citrus nobilis lauriro) peel oil.volatiles of a wild mandarin, mangshanyegan (citrus nobilis lauriro), were characterized by gc-ms, and their aroma active compounds were identified by aroma extract dilution analysis (aeda) and gas chromatography-olfactometry (gc-o). the volatile profile of mangshanyegan was compared with those of other four citrus species, kaopan pummelo (citrus grandis), eureka lemon (citrus limon), huangyanbendizao tangerine (citrus reticulata), and seike navel orange (citrus sinensis). monoterpene hydrocarbo ...201222352344
ozone deposition to an orange orchard: partitioning between stomatal and non-stomatal sinks.orange trees are widely cultivated in regions with high concentrations of tropospheric ozone. citrus absorb ozone through their stomata and emit volatile organic compounds (voc), which, together with soil emissions of no, contribute to non-stomatal ozone removal. in a valencia orange orchard in exeter, california, we used fast sensors and eddy covariance to characterize water and ozone fluxes. we also measured meteorological parameters necessary to model other important sinks of ozone deposition ...201222341155
unravelling molecular responses to moderate dehydration in harvested fruit of sweet orange (citrus sinensis l. osbeck) using a fruit-specific aba-deficient mutant.water stress affects many agronomic traits that may be regulated by the phytohormone abscisic acid (aba). within these traits, loss of fruit quality becomes important in many citrus cultivars that develop peel damage in response to dehydration. to study peel dehydration transcriptional responsiveness in harvested citrus fruit and the putative role of aba in this process, this study performed a comparative large-scale transcriptional analysis of water-stressed fruits of the wild-type navelate ora ...201222315241
differential expression of genes involved in alternative glycolytic pathways, phosphorus scavenging and recycling in response to aluminum and phosphorus interactions in citrus roots.the objective was to determine the possible links between the expression levels of genes involved in alternative glycolytic pathways, phosphorus (p) scavenging and recycling and citrus tolerance to aluminum (al) and/or p-deficiency. 'xuegan' (citrus sinensis) and 'sour pummelo' (citrus grandis) seedlings were irrigated for 18 weeks with nutrient solution containing 0 and 1.2 mm alcl(3)·6h(2)o × 0, 50 and 200 μm kh(2)po(4). c. sinensis displayed more tolerant to al and p-deficiency than c. grandi ...201222307782
Refresh
firstprev
12345678910...
nextlast
Displaying items 501 - 600 of 1248