Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| no simple association between time elapsed from seroconversion until slaughter and the extent of lung lesions in danish swine. | the association between the extent of pathological lung lesions at slaughter and the time elapsed from seroconversion to slaughter was examined in a longitudinal study including 830 pigs from eight herds. pigs from an age of 3 weeks were bled every fourth week, and the sera were analyzed for the presence of antibodies to mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae serotypes 2, 5, 6, 7 and 12. at slaughter, the extent of mycoplasma-like catharral pneumonia, chronic pleuritis (dor ... | 2001 | 11679172 |
| improved vectors for expression library immunization--application to mycoplasma hyopneumoniae infection in pigs. | expression library immunization (eli) has previously been used in a number of disease models in mice. here, we describe the first example of the application of eli to a large animal model with the immunization of pigs against enzootic pneumonia, a disease caused by mycoplasma hyopneumoniae. the development of new plasmid vectors and library screening methods facilitated the application of eli to this disease by allowing random libraries to be screened for clones expressing recombinant proteins. ... | 2001 | 11567754 |
| a physical and genetic map of the mycoplasma flocculare atcc 27716 chromosome reveals large genomic inversions when compared with that of mycoplasma hyopneumoniae strain j(t). | pulsed-field gel electrophoresis and dna hybridization data were used to construct a chromosomal map of mycoplasma flocculare atcc 27716, a non-pathogenic inhabitant of porcine respiratory tracts. twenty-one genetic markers were placed on the map. comparison of the genetic map with that of the closely related mycoplasma hyopneumoniae strain j(t), the type strain of the causative agent of enzootic pneumonia in pigs, identified three chromosomal inversions that differentiate these genomes. one of ... | 2001 | 11491338 |
| interaction between mycoplasma hyopneumoniae and swine influenza virus. | an experimental respiratory model was used to investigate the interaction between mycoplasma hyopneumoniae and swine influenza virus (siv) in the induction of pneumonia in susceptible swine. previous studies demonstrated that m. hyopneumoniae, which produces a chronic bronchopneumonia in swine, potentiates a viral pneumonia induced by the porcine reproductive and respiratory syndrome virus (prrsv). in this study, pigs were inoculated with m. hyopneumoniae 21 days prior to inoculation with siv. c ... | 2001 | 11427564 |
| differential production of proinflammatory cytokines: in vitro prrsv and mycoplasma hyopneumoniae co-infection model. | an in vitro culture system was developed to investigate the induction of proinflammatory cytokines by mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus (prrsv). m. hyopneumoniae infected porcine tracheal ring explants were co-cultured with prrsv infected pulmonary alveolar macrophages (pams) for 24h to assess the cytokine production of each pathogen alone and the interaction between the two pathogens in vitro. semiquantitative rt-pcr was used to measure interleukin ... | 2001 | 11356254 |
| a recombinant chimera composed of repeat region rr1 of mycoplasma hyopneumoniae adhesin with pseudomonas exotoxin: in vivo evaluation of specific igg response in mice and pigs. | using the binding and translocation domain of pseudomonas exotoxin a [domain iii deleted pe termed pe(deltaiii)] as a vehicle, this study characterized and evaluated a novel application of pe toxin in mycoplasma hyopneumoniae adhesin used as an immunogen. pcr and sequence analysis revealed that 16 copies of aakpv(e) in tandem repeat region 1 (rr1) of m. hyopneumoniae 97kda adhesion were successfully fused to the downstream of pe(deltaiii) to create a subunit vaccine, i.e. pe(deltaiii)-rr1. this ... | 2001 | 11348771 |
| colonisation state and colostral immunity to mycoplasma hyopneumoniae of different parity sows. | 2000 | 11321216 | |
| no overall relationship between average daily weight gain and the serological response to mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae in eight chronically infected danish swine herds. | the association between the average daily weight gain (from approximately 4 to 20 weeks of age) and the serological responses to respiratory infections was examined in a longitudinal study including 825 pigs from eight chronically infected herds. pigs were bled every 4th week (starting from approximately 4 weeks of age), and sera were analyzed for antibodies to mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae serotypes 2, 5-7 and 12.mixed analysis of covariance analyzed the relations ... | 2001 | 11267685 |
| aspects of the transmission of protection against mycoplasma hyopneumoniae from sow to offspring. | the aims of this study were to describe the variation in concentration of antibodies to mycoplasma hyopneumoniae in the serum and colostrum of sows, and to compare the amount of antibodies in colostrum with that obtained in the serum of the smallest piglets in a litter. in addition, the efficacy of the passive immunity in natural conditions was studied. the study was performed in a sow pool herd (600 sows) that was endemically infected with m. hyopneumoniae. blood samples were collected from sow ... | 2001 | 11254099 |
| seroepidemiology of mycoplasma hyopneumoniae in pigs from farrow-to-finish farms. | a prospective study was carried out on three intensive farrow-to-finish farms. the aims were to estimate the incidence of mycoplasma hyopneumoniae infection, to determine when pigs become infected and the pattern of transmission of infection and to verify the relationship between seroconversion and clinical signs. one batch of pigs per farm was followed from farrowing-to-slaughter. blood samples were taken at 10, 27, 70, 94, 125 and 147 days of age, from 44, 48 and 44 pigs per farm. colostrum an ... | 2001 | 11182499 |
| oral immunization of swine with attenuated salmonella typhimurium aroa sl3261 expressing a recombinant antigen of mycoplasma hyopneumoniae (nrdf) primes the immune system for a nrdf specific secretory iga response in the lungs. | salmonella typhimurium sl3261 (aroa mutant) expressing a recombinant mycoplasma hyopneumoniae antigen was used to orally immunize swine against porcine enzootic pneumonia. this construct, designated s. typhimurium aro a sl3261 (pkf1), expressed a recombinant protein containing the carboxy-terminal 11 kda of a 42 kda m. hyopneumoniae nrdf ribonucleotide reductase r2 subunit protein. here we demonstrate that this antigen is present in all seven geographically diverse strains of m. hyopneumoniae te ... | 2001 | 11162190 |
| monitoring antibodies to mycoplasma hyopneumoniae in sow colostrum--a tool to document freedom of infection. | in a survey in finland in 1995, 14,919 colostral whey samples from 530 farrowing herds were analysed by a monoclonal blocking-elisa to detect antibodies to mycoplasma hyopneumoniae (m. hyopneumoniae). antibodies were detected in 274 (1.8%) samples and in 42 herds (7.9%). the median prevalence of sows with antibodies in seropositive herds was 28.2% (range, 2.7-100%). according to clinical and pathological follow-up in finishing herds in 1996, all of the farrowing herds which were seronegative in ... | 2000 | 11126571 |
| evaluation of local and systemic immune responses induced by intramuscular injection of a mycoplasma hyopneumoniae bacterin to pigs. | to evaluate immune responses induced by administration of mycoplasma hyopneumoniae bacterin to pigs. animals-60 healthy 7- to 10-day-old cross-bred boars. | 2000 | 11108184 |
| use of an internal control in a nested-pcr assay for mycoplasma hyopneumoniae detection and quantification in tracheobronchiolar washings from pigs. | we have previously reported a nested pcr assay for the detection of mycoplasma hyopneumoniae directly in tracheobronchiolar washings from living pigs in field conditions. here, we describe the construction and use of an internal control to monitor the presence of pcr inhibitors. a pcr modified target dna was constructed by insertion of a small dna fragment into the m. hyopneumoniae specific dna target. we have demonstrated that the internal control failed to be amplified in only three tracheobro ... | 2000 | 11090266 |
| protective effect of vaccination with culture supernate of m. hyopneumoniae against experimental infection in pigs. | the protective activity of mycoplasma hyopneumoniae inactivated vaccine prepared from sedimented whole cells and cell-free culture supernates was evaluated experimentally using hysterectomy-produced, colostrum-deprived pigs in which mycoplasmal pneumonia had been induced. the culture supernate vaccine containing less than 10(1) colour-changing units (ccu)/0.2 ml of m. hyopneumoniae significantly (p < 0.05) reduced the percentage of lung lesions compared to controls (3.2 +/- 3.9 vs. 12.2 +/- 2.2% ... | 2000 | 11048434 |
| varying effects of infections with mycoplasma hyopneumoniae on the weight gain recorded in three different multisource fattening pig herds. | pigs in three specialized fattening herds were studied with respect to the effect of infection with mycoplasma hyopneumoniae on weight gain. individual pigs were weighed four times at 4-week intervals during the fattening period and their daily weight gain over the rearing period was calculated. a blood sample was collected on each weighing occasion and analysed for the presence of antibodies to m. hyopneumoniae. the lungs of the principals were inspected at slaughter and the extent of pneumonic ... | 2000 | 11014068 |
| a physical and genetic map of the mycoplasma hyopneumoniae strain j genome. | a macrorestriction map of the genome of mycoplasma hyopneumoniae strain j, the type strain of the causative agent of enzootic pneumonia in pigs, was constructed using pulsed-field gel electrophoresis (pfge) and dna hybridization. the size of the genome as determined by pfge was approximately 1070 kb. assembly of the m. hyopneumoniae genomic map was facilitated and complimented by the simultaneous construction of an ordered cosmid library. five contigs of overlapping cosmids were assembled, which ... | 2000 | 11006844 |
| comparison of serologic testing and slaughter evaluation for assessing the effects of subclinical infection on growth in pigs. | to compare serologic testing with slaughter evaluation in assessing effects of subclinical infection on average daily weight gain (adg) in pigs. | 2000 | 10997163 |
| correlation between the presence of enzootic pneumonia lesions and detection of mycoplasma hyopneumoniae in bronchial swabs by pcr. | in many diagnostic laboratories the diagnosis of mycoplasmal pneumonia in pigs is based on clinical signs and the presence of gross and histopathological lesions. the objective of this study was to evaluate the nested-pcr technique as an adjunct to the histopathological diagnosis of mycoplasma hyopneumoniae infection. respiratory disease of 184 swine cases submitted to the minnesota veterinary diagnostic laboratory between 1 january and 30 june 1998 were used. bronchial swabs were collected and ... | 2000 | 10973704 |
| field evaluation of efficacy and tolerance of a 2% marbofloxacin injectable solution for the treatment of respiratory disease in fattening pigs. | in a controlled and randomized field trial carried out in three european countries, 219 fattening pigs, from seven farms with respiratory disease problems, were treated intramuscularly for three or five days either with marbofloxacin 2 mg/kg/day, or with amoxicillin 7 mg/kg/day. pigs were monitored daily until d5 (day 5) and again at d21, and were weighed at d0 and d21. pasteurella multocida, actinobacillus pleuropneumoniae, and mycoplasma hyopneumoniae were mainly identified in the pig lungs. t ... | 2000 | 10952441 |
| [effect of the stellamune mycoplasma vaccine on growth, energy conversion, death, and medication use in fattening pigs on a pig farm chronically infected with mycoplasma hyopneumoniae]. | the effect of stellamune mycoplasma vaccine, administered to piglets aged 2-15 days and then 13-15 days later, on daily weight gain, energy conversion, and use of medication was examined in fattening pigs on a chronically mycoplasma hyopneumoniae infected pig farm. half of the piglets were vaccinated and the other half acted as controls. in the study design, half of the pens in the fattening unit were allocated to vaccinated pigs; the other half to non-vaccinated pigs, pen was the experimental u ... | 2000 | 10948597 |
| a nested pcr assay for the detection of mycoplasma hyopneumoniae in tracheobronchiolar washings from pigs. | a nested polymerase chain reaction (pcr) was developed for the detection of mycoplasma hyopneumoniae, the etiological agent of enzootic pneumonia, in tracheobronchiolar washings from live pigs. two nested pairs of oligonucleotide primers were designed from the sequence of a specific dna probe (i 141; accession number u02537). the primer combination was hp1/hp3 for the first step pcr while the nested primers (hp4/hp6) allowed amplification of a 706 bp fragment. all strains of m. hyopneumoniae tes ... | 2000 | 10925039 |
| molecular cloning and characterization of a unique 60 kda/72 kda antigen gene encoding enzyme i of the phosphoenolpyruvate: sugar phosphotransferase system (pts) of mycoplasma hyopneumoniae. | the recombinant clone expressing a 60 kda (p60) antigen was isolated from escherichia coli by screening a lambda embl3 genomic library using rabbit produced antiserum against mycoplasma hyopneumoniae. sequence analysis revealed that an interrupted (by a uga codon) open reading frame coding for a 72 kda protein (p72) may contain the p60 antigen gene. western blot analysis with an anti-p60 monospecific antibody confirmed the presence of a p72 antigen from the total protein of m. hyopneumoniae, and ... | 2000 | 10920262 |
| species-specific monoclonal antibodies to escherichia coli-expressed p36 cytosolic protein of mycoplasma hyopneumoniae. | the p36 protein of mycoplasma hyopneumoniae is a cytosolic protein carrying species-specific antigenic determinants. based on the genomic sequence of the reference strain atcc 25934, primers were designed for pcr amplification of the p36-encoding gene (948 bp). these primers were shown to be specific to m. hyopneumoniae since no dna amplicons could be obtained with other mycoplasma species and pathogenic bacteria that commonly colonize the porcine respiratory tract. the amplified p36 gene was su ... | 2000 | 10882646 |
| immunoblot assays using recombinant antigens for the detection of mycoplasma hyopneumoniae antibodies. | the 36kda l-lactate dehydrogenase (ldh) and a 29kda partial fragment of an abc transporter atp-binding protein analogue/multidrug resistance protein homologue (pr2) of mycoplasma hyopneumoniae were tested for their potential as diagnostic antigens. recombinant ldh was genetically engineered to contain six histidine residues at its c-terminal end, expressed in escherichia coli and purified to a high degree using ni(2+)-chelate affinity chromatography. a partial 262 amino acid segment representing ... | 2000 | 10865156 |
| herd factors associated with the seroprevalences of four major respiratory pathogens in slaughter pigs from farrow-to-finish pig herds. | the objective of this study was to investigate sero-epidemiological aspects of mycoplasma hyopneumoniae (mh), influenza h1n1 and h3n2 viruses and aujeszky disease virus (adv) in fattening pigs from 150 randomly selected farrow-to-finish pig herds. different herd factors were examined as potential risk indicators for the percentage of pigs with antibodies against the 4 pathogens. the median within-herd seroprevalences of the pathogens were: mh 76%, h1n1 100%, h3n2 40% and adv 53%. there was a pos ... | 2000 | 10863948 |
| lack of effect of aerial ammonia on atrophic rhinitis and pneumonia induced by mycoplasma hyopneumoniae and toxigenic pasteurella multocida. | the objective of this experimental study was to determine the effects of aerial ammonia on disease development and bacterial colonization in weaned pigs inoculated with toxigenic pasteurella multocida and mycoplasma hyopneumoniae. two groups of 10 pigs each were continuously exposed to 50 and 100 p.p.m. ammonia, respectively, and compared to a non-exposed control group of 20 pigs. following aerosol inoculation with m. hyopneumoniae at day 9, all pigs were aerosol-inoculated with toxigenic p. mul ... | 2000 | 10829570 |
| a longitudinal study of serological patterns of respiratory infections in nine infected danish swine herds. | sixteen litters of seven pigs from each of nine danish farrow-to-finish herds were followed to investigate the serological patterns caused by natural infection with mycoplasma hyopneumoniae, pasteurella multocida toxin and actinobacillus pleuropneumoniae serotypes 2, 5-7, 12. in seven of the herds, pigs were followed as two separate cohorts started 4 weeks apart, and in two herds only one cohort was followed.a total of 999 pigs were included in the study. the pigs were blood sampled at weaning a ... | 2000 | 10821962 |
| cytological and immunological changes in bronchoalveolar lavage fluid and histological observation of lung lesions in pigs immunized with mycoplasma hyopneumoniae inactivated vaccine prepared from broth culture supernate. | we have compared the cytology of bronchoalveolar lavage fluid (balf) and the pathology of lung lesions in pigs immunized with/without mycoplasma hyopneumoniae inactivated vaccine prepared from broth culture supernate on experimental infection. numbers of total cells, macrophages, neutrophils and lymphocytes have decreased in balf of vaccinated pigs following infection. the mean percentage of lung lesions, inflammatory cell infiltration into the airways and t cells accumulation around the bronchi ... | 2000 | 10812225 |
| identification of the copper-zinc superoxide dismutase activity in mycoplasma hyopneumoniae. | copper-zinc superoxide dismutase (cu/znsod), a key enzyme in defense against toxic oxygen-free radicals, is widespread in eukaryotes and several species of gram-negative bacteria. the presence of this enzyme in mycoplasma hyopneumoniae (m. hyopneumoniae), the primary pathogen of mycoplasmal pneumonia in pigs, was examined since the polyclonal antibody against bovine cu/znsod was dominantly cross-reactive with the m. hyopneumoniae cu/znsod from whole cellular proteins. in situ activity staining o ... | 2000 | 10781728 |
| r1 region of p97 mediates adherence of mycoplasma hyopneumoniae to swine cilia. | adherence of mycoplasma hyopneumoniae to the swine respiratory tract is mediated by the membrane protein p97. this protein is located on the outer membrane surface, and its role in adherence has been firmly established. the general region of p97 that mediates adherence to swine cilia is thought to be the r1 region near the carboxy terminus of the protein, but it was not clear if this region could mediate adherence to swine cilia independently of other p97 sequences. to examine this in more detai ... | 2000 | 10769015 |
| diagnosis and differentiation of mycoplasma hyopneumoniae and mycoplasma hyorhinis infections in pigs by pcr amplification of the p36 and p46 genes. | the genome of mycoplasma hyopneumoniae encodes several immunodominant proteins, including a cytosolic protein (p36), three membranous proteins (p46, p65, and p74), and an adhesin (p97). cross-reactions with m. flocculare and m. hyorhinis reduce the specificity of conventional serological detection methods. however, certain antigenic determinants of the p36 and p46 proteins have been shown to be specific for m. hyopneumoniae. in the present study, pairs of oligonucleotide primers were designed to ... | 2000 | 10747113 |
| a tissue culture system to study respiratory ciliary epithelial adherence of selected swine mycoplasmas. | an in vitro culture system for swine tracheal epithelial cells was developed to study the adherence of swine mycoplasmas. swine tracheal epithelial cells were isolated by enzymatic digestion and cultured on microporous membranes. growth medium was placed under the membrane support to create air-liquid interface feeding resulting in the cells growing cilia and microvilli on the apical surface. two strains of mycoplasma hyopneumoniae (pathogenic strain 91-3 and non-pathogenic type strain j) and tw ... | 2000 | 10703709 |
| effect of vaccination on the potentiation of porcine reproductive and respiratory syndrome virus (prrsv)-induced pneumonia by mycoplasma hyopneumoniae. | porcine reproductive and respiratory syndrome virus (prrsv) and mycoplasma hyopneumoniae are frequently isolated pathogens from pigs with respiratory disease. a previous study conducted in our laboratory found that infection with m. hyopneumoniae increased the duration and severity of respiratory disease induced by prrsv. the purpose of this experiment was to determine whether vaccination against m. hyopneumoniae and/or prrsv decreased the enhancement of prrsv-induced pneumonia. both m. hyopneum ... | 2000 | 10649626 |
| eradication of mycoplasma hyopneumoniae from infected swine herds joining the lso 2000 health class. | the study was conducted in order to determine if eradication of swine enzootic pneumoniae (sep) had succeeded with different variants of partial depopulation during the eradication programme on swine farrowing farms joining a health class, lso 2000. the farms in the health class need to be free from swine enzootic pneumoniae, swine dysentery, sarcoptic mange and atrophic rhinitis. twenty-one eradication attempts for m. hyopneumoniae were carried out using different variants based on separating a ... | 1999 | 10605141 |
| infections with mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae in fattening pigs. influence of piglet production systems and influence on production parameters. | two hundred and sixty-four feeder pigs from an age-segregated herd (a-pigs) and 264 feeder pigs from a continuous production system (c-pigs) were transferred into identical but separate rooms in a fattening herd employing all-in all-out production. on arrival, none of the a-pigs and 39% of the c-pigs were seropositive to mycoplasma hyopneumoniae (m. hyo). at slaughter 30% of the a-pigs and 99% of the c-pigs were seropositive to m. hyo. pigs with acute swine enzootic pneumonia (sep) at slaughter ... | 1999 | 10574070 |
| evaluation of mycoplasma hyopneumoniae inactivated vaccine in pigs under field conditions. | an inactivated vaccine prepared from broth culture supernatant of mycoplasma hyopneumoniae with an aluminum adjuvant was evaluated in three herds (herd a: specific pathogen-free herd, herd b: high health status herd with no clinical signs of respiratory infection, herd c: low health status herd with serious epidemiological and economical problems). a total of 212 pigs from the three herds were divided into two groups. one group was injected twice with the vaccine at 4-week intervals and the othe ... | 1999 | 10563291 |
| amplified-fragment length polymorphism fingerprinting of mycoplasma species. | amplified-fragment length polymorphism (aflp) is a whole-genome fingerprinting method based on selective amplification of restriction fragments. the potential of the method for the characterization of mycoplasmas was investigated in a total of 50 strains of human and animal origin, including mycoplasma genitalium (n = 11), mycoplasma pneumoniae (n = 5), mycoplasma hominis (n = 5), mycoplasma hyopneumoniae (n = 9), myco plasma flocculare (n = 5), mycoplasma hyosynoviae (n = 10), and mycoplasma di ... | 1999 | 10488196 |
| detection and localization of mycoplasma hyopneumoniae dna in lungs from naturally infected pigs by in situ hybridization using a digoxigenin-labeled probe. | mycoplasma hyopneumoniae dna was detected in 20 naturally infected pigs by in situ hybridization using a nonradioactive digoxigenin-labeled dna probe. a 520-base-pair dna probe targeting a reiterative sequence of the m. hyopneumoniae genome was generated by the polymerase chain reaction. all 20 pigs infected with m. hyopneumoniae had distinct and positive hybridization signals without background staining. a strong hybridization signal was detected mainly in the luminal surface of bronchial and b ... | 1999 | 10421097 |
| risk indicators for the seroprevalence of mycoplasma hyopneumoniae, porcine influenza viruses and aujeszky's disease virus in slaughter pigs from fattening pig herds. | epidemiological aspects of mycoplasma hyopneumoniae (mh), influenza h1n1 and h3n2 viruses, and aujeszky's disease virus (adv) were investigated in slaughter pigs from 50 fattening pig herds. herd factors as potential risk indicators for respiratory disease were obtained by means of a questionnaire. at slaughter, blood samples were collected from each herd, and the proportion of seropositive pigs per herd was assessed for each of these pathogens. the median herd-level seroprevalence of the agents ... | 1999 | 10416368 |
| application of a nested polymerase chain reaction assay to detect mycoplasma hyopneumoniae from nasal swabs. | the porcine respiratory disease complex (prdc) is an increasingly important cause of decreased swine productivity and is characterized by slow growth, decreased feed efficiency, anorexia, cough, and dyspnea. mycoplasma hyopneumoniae is among the most prevalent and important infectious agents associated with prdc. understanding of mycoplasmal pneumonia has been hindered by inadequate diagnostic methods. many of the currently available tests are relatively insensitive or nonspecific when used in a ... | 1999 | 10353356 |
| health and growth performance of barrows reared in all-in/all-out or continuous flow facilities with or without a chlortetracycline feed additive. | to compare health and growth performance in barrows reared in all-in/all-out (aiao) or continuous flow (cf) management systems. | 1999 | 10328431 |
| translational infidelity and human cancer: role of the pti-1 oncogene. | several components of the eukaryotic protein synthesis apparatus have been associated with oncogenic transformation of cells. altered expression of translation elongation factor 1 alpha (ef-1 alpha), a core component of protein synthesis and closely related sequences have been linked with transformed phenotypes by several independent studies, in diverse systems. a dominant acting oncogene, prostate tumor inducing gene-1 (pti-1) has provided further evidence for this link. pti-1 appears to be a h ... | 1999 | 10216950 |
| effect of vaccination against mycoplasma hyopneumoniae in pig herds with an all-in/all-out production system. | a multi-site field study was conducted to evaluate an inactivated mycoplasma hyopneumoniae (mh) vaccine in 14 pig herds infected by mh and practising an all-in/all-out production system. in each herd, a vaccinated and control group of 250 pigs each were compared during the growing/finishing period with respect to performance parameters (major variables) and by means of clinical, serological and pathological parameters (ancillary variables). mh vaccination significantly (p < 0.05) improved daily ... | 1999 | 10195611 |
| field efficacy of a combined use of mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae vaccines in growing pigs. | the effectiveness of simultaneous administration of commercial mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae vaccines was tested in an indoor commercial piggery which had experienced continuing respiratory-disease problems confirmed as due to both of these pathogens. piglets were randomly assigned in equal numbers to vaccination and control groups, and each vaccine was administered at a separate site to assigned piglets at two and four weeks of age. live weight of vaccinates immed ... | 1999 | 10081785 |
| an on-farm study of the epidemiology of actinobacillus pleuropneumoniae infection in pigs as part of a vaccine efficacy trial. | thirty cohort pigs were followed from birth to slaughter to study epidemiological patterns of porcine pleuropneumonia caused by actinobacillus pleuropneumoniae. the study was conducted within a larger 380-animal study of vaccination against mycoplasma hyopneumoniae and a. pleuropneumoniae in a 340-sow farrow-to-finish piggery with 4-month weaning, operating a continuous system of intensive production in the north island of new zealand. the cohort pigs were randomly allocated into two equal group ... | 1999 | 10081784 |
| mycoplasma hyopneumoniae potentiation of porcine reproductive and respiratory syndrome virus-induced pneumonia. | an experimental model that demonstrates a mycoplasma species acting to potentiate a viral pneumonia was developed. mycoplasma hyopneumoniae, which produces a chronic, lymphohistiocytic bronchopneumonia in pigs, was found to potentiate the severity and the duration of a virus-induced pneumonia in pigs. pigs were inoculated with m. hyopneumoniae 21 days prior to, simultaneously with, or 10 days after inoculation with porcine reproductive and respiratory syndrome virus (prrsv), which induces an acu ... | 1999 | 9986823 |
| [results of field studies using stellamune mycoplasma vaccine in luxembourg]. | enzootic pneumonia caused by mycoplasma hyopneumoniae is responsible for major economic losses in pig herds worldwide. m. hyopneumoniae can also act as a primary agent of swine respiratory disease followed by bacterial or viral infections. vaccines have been developed to prevent enzootic pneumoniae. in this experiment, an inactivated vaccine against mycoplasma, also sold under the name stellamune mycoplasma (pfizer animal health) was used in 2 farms with a known history of m. hyopneumoniae infec ... | 1998 | 9868832 |
| the effect of vaccination against mycoplasma hypopneumoniae in pig herds with a continuous production system. | an inactivated mycoplasma hyopneumoniae vaccine was evaluated in five pig herds clinically infected with enzootic pneumonia and practising a continuous production system in the growing/finishing unit. in each herd, a vaccinated and control group of approximately 47 pigs each were individually monitored from birth until slaughter. vaccinated pigs received the first dose at about 1 week of age and the second approximately 3 weeks later. during all production stages, an equal number of vaccinated a ... | 1998 | 9820117 |
| rnase p rna structure and cleavage reflect the primary structure of trna genes. | the function of rnase p rna depends on its folding in space. a majority of rnase p rnas from various bacteria show a similar secondary structure to that of escherichia coli (m1 rna). however, there are exceptions as exemplified by the rnase p rna derived from the low gc-content gram-positive bacteria bacillus subtilis and mycoplasma hyopneumoniae (hyo p rna). previous studies using m1 rna and hyo p rna suggest differences both with respect to the kinetics of cleavage as well as to cleavage site ... | 1998 | 9790839 |
| the prevalence of mycoplasma hyopneumoniae in pig herds in western finland based on the demonstration of antibodies in colostrum by elisa. | swine enzootic pneumonia causes heavy economical losses in fattening herds. the aim of this study was to get an estimate of the prevalence of mycoplasma hyopneumoniae in the province of vaasa in western finland. there were 112 farrowing herds randomly chosen for a survey. farmers were asked to milk colostrum samples from every sow. owners of 22 herds did not send enough samples for a herd diagnosis. altogether 1773 samples (mean 18.1 samples/herd) were analysed in an indirect elisa for antibodie ... | 1998 | 9787495 |
| efficacy of doxycycline in feed for the control of pneumonia caused by pasteurella multocida and mycoplasma hyopneumoniae in fattening pigs. | a multicentre, controlled, randomised and blinded study was carried out in three french pig herds to assess the efficacy of doxycycline administered in the feed for the control of pneumonia. about 20 per cent of 363 pigs from the three fattening units were diseased at the start of the study. pneumonic lesions were found on pigs examined postmortem and pasteurella multocida was isolated from the lungs of pigs in all the herds. mycoplasma hyopneumoniae infection was confirmed either by detection i ... | 1998 | 9787419 |
| identification of the cilium binding epitope of the mycoplasma hyopneumoniae p97 adhesin. | mycoplasma hyopneumoniae colonizes the swine respiratory tract at the level of ciliated cells by attaching specifically to the cilium membrane. this interaction involves an adhesin called p97; the cilium binding activity of this protein was localized to the carboxy terminus, which included two repeat regions, r1 and r2 (t. hsu, s. artiushin, and f. c. minion, j. bacteriol. 179:1317-1323, 1997). to further delineate the molecular mechanisms of m. hyopneumoniae interactions with ciliated epitheliu ... | 1998 | 9746576 |
| molecular analysis of the p97 cilium adhesin operon of mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae causes an economically significant respiratory disease of swine called enzootic pneumonia. the disease process is initiated by adherence of m. hyopneumoniae to the cilia of swine respiratory epithelium through an interaction involving p97, a surface-associated protein, and cilia-specific receptors. binding specificity is associated with a repeat region located near the c-terminus of the p97 protein. further analysis of the dna sequences surrounding the p97 structural gen ... | 1998 | 9729120 |
| reiterated repeat region variability in the ciliary adhesin gene of mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae is a highly prevalent pathogen which colonizes the ciliated epithelial lining of the porcine respiratory tract. expression libraries constructed from genomic dna of the non-pathogenic strain m. hyopneumoniae j were screened with porcine hyperimmune antiserum against m. hyopneumoniae. one clone expressed a 28 kda protein which was also reactive with monospecific antiserum raised against a putative m. hyopneumoniae-specific 94 kda antigen derived from strain j. trypsin dig ... | 1998 | 9695926 |
| identification of a novel adhesin-like glycoprotein from mycoplasma hyopneumoniae. | this study identifies an adhesin-like glycoprotein, which was a 110 kda protein (p110) under hplc-gpc assay. this adhesin consisted of one p54 and two p28 subunits. in addition, n-glycosidase f could cleave all n-linked oligosaccharides on the p54 subunit. experimental results indicated that p110 with native conformations significantly inhibited the adherence of biotin-labeled porcine tracheal epithelial cell extracts to the intact m. hyopneumoniae cells (p < 0.01). furthermore, the biotin-label ... | 1998 | 9695283 |
| detection of mycoplasma hyopneumoniae in bronchoalveolar lavage fluids of pigs by pcr. | in the present investigation we developed a method for the detection of mycoplasma hyopneumoniae in bronchoalveolar lavage fluid (balf) of pigs by pcr with a primer pair flanking a dna fragment of 853 bp specific for m. hyopneumoniae. several methods were tested to eliminate the amplification inhibitors present in balfs. the best results were obtained by the extraction of the dna from the balfs. by the pcr performed with the extracted dna, 10(2) cfu of m. hyopneumoniae could be detected in 1 ml ... | 1998 | 9650949 |
| humoral immune responses to mycoplasma hyopneumoniae in sows and offspring following an outbreak of mycoplasmosis. | previously healthy sows, seropositive to mycoplasma hyopneumoniae, developed clinical signs of mycoplasmosis, as well as increasing amounts of antibodies to m. hyopneumoniae during an outbreak of the disease in a herd. during the early phase of the outbreak, young piglets (2 weeks) with maternal antibodies remained healthy while older seronegative piglets (4-7 weeks) developed the disease. the duration of the maternal antibodies to m. hyopneumoniae varied between litters and was related to the a ... | 1998 | 9646450 |
| relationships between selected climatic factors in fattening units and their influence on the development of respiratory diseases in swine. | the correlation between climatic parameters with one another in fattening units and the influence of environmental factors on lung lesions registered at slaughter were studied in 6 integrated herds with continuous production systems. in addition, the influence of environmental parameters on the spread of mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae (serotypes 2 and 3) and on the productivity was monitored in 2 specialized fattening herds with strict batch production. the outdoor ... | 1998 | 9592945 |
| experimental dual infection of cesarean-derived, colostrum-deprived pigs with mycoplasma hyopneumoniae and pseudorabies virus. | to determine whether pseudorabies virus (prv) infection increases the severity of pneumonia by mycoplasma hyopneumoniae, 18, 10-week-old cesarean-derived, colostrum-deprived pigs were randomly assigned to 3 groups of 6 pigs each. pigs in groups a and c were inoculated intranasally with m. hyopneumoniae at 10-week-old. at 11-week-old, pigs in groups b and c were inoculated intranasally with prv. all pigs were initially seronegative for m. hyopneumoniae and prv. three pigs of each group were eutha ... | 1998 | 9560775 |
| antisense inhibition of the pti-1 oncogene reverses cancer phenotypes. | the genetic alterations and molecular events mediating human prostate cancer development and progression remain to be defined. rapid expression cloning and differential rna display detect a putative oncogene, prostate tumor-inducing gene 1 (pti-1), that is differentially expressed in human prostate (as well as breast, colon, and small cell lung) cancer cell lines, patient-derived prostate carcinomas, and blood from patients with metastatic prostate cancer. pti-1 consists of a unique 5' untransla ... | 1998 | 9465091 |
| detection of mycoplasma hyopneumoniae by air sampling with a nested pcr assay. | this article describes the first successful detection of airborne mycoplasma hyopneumoniae under experimental and field conditions with a new nested pcr assay. air was sampled with polyethersulfone membranes (pore size, 0.2 micron) mounted in filter holders. filters were processed by dissolution and direct extraction of dna for pcr analysis. for the pcr, two nested pairs of oligonucleotide primers were designed by using an m. hyopneumoniae-specific dna sequence of a repeated gene segment. a nest ... | 1998 | 9464391 |
| in vitro susceptibilities of recent field isolates of mycoplasma hyopneumoniae and mycoplasma hyosynoviae to valnemulin (econor), tiamulin and enrofloxacin and the in vitro development of resistance to certain antimicrobial agents in mycoplasma hyopneumoniae. | the in vitro activities of valnemulin (econor) and two other antimicrobial agents were determined against recent field strains of mycoplasma hyopneumoniae and mycoplasma hyosynoviae using a broth microdilution method. valnemulin showed exceptional activity against m hyopneumoniae (mic90 0.0005 microgram ml-1) and m hyosynoviae (mic range 0.0001 microgram ml-1 to 0.00025 microgram ml-1) field strains. tiamulin was 100-fold less active (mic90 0.05 microgram ml-1) and enrofloxacin 20-fold less acti ... | 1997 | 9429250 |
| gene amplification (pcr) to detect and differentiate mycoplasmas in porcine mycoplasmal pneumonia. | the causative agent of porcine mycoplasmal pneumonia, mycoplasma hyopneumoniae, is difficult and time-consuming to isolate. serological identification using antibodies induced by the disease is confused by cross-reaction with a closely related organism, myc. flocculare. from pig lungs obtained at slaughter for meat and deemed free of acute disease, it was possible to detect by culture both myc. hyopneumoniae and myc. flocculare. this study has improved on an earlier pcr detection of dna from the ... | 1997 | 9418066 |
| association of tumour necrosis factor and acute phase reactant changes with post arrival disease in swine. | severe weight loss in the absence of respiratory, enteric or systemic clinical disease or gross pathologic lesions is often observed when immunologically naive boars are placed in conventional health swine facilities. affected animals develop this weight loss in spite of receiving pre-entry vaccinations against common swine pathogens, such as haemophilus parasuis or mycoplasma hyopneumoniae. in many cases, the weight loss is non-responsive to long term antibiotic therapy. in order to determine t ... | 1997 | 9323928 |
| microbiology and pathology of fibrinous pericarditis in danish slaughter pigs. | the prevalence of fibrinous pericarditis detected at slaughter in danish slaughter pigs is approximately 0.02%. the microbiology and pathology of this disorder was studied through 46 field cases collected at slaughter from may 1994 to august 1995. mycoplasmas (mycoplasma hyopneumoniae, m. hyosynoviae and m. hyorhinis) were isolated from the pericardium in 38 cases and from the joints in six. m. hyopneumoniae dominated with 33 isolates from the pericardium. actinobacillus pleuropneumoniae was iso ... | 1997 | 9274146 |
| serum and mucosal antibody responses and protection in pigs vaccinated against mycoplasma hyopneumoniae with vaccines containing a denatured membrane antigen pool and adjuvant. | to investigate the protective efficacy of a pool of denatured membrane protein antigens of mycoplasma hyopneumoniae (j strain) in the molecular size range 70 to 85 kda (f3 antigen) in combination with adjuvants for pigs challenged with m hyopneumoniae. | 1997 | 9258425 |
| comparative in vitro activity of doxycycline and oxytetracycline against porcine respiratory pathogens. | minimum inhibitory concentrations of doxycycline and oxytetracycline were determined against 55 pasteurella multocida strains, 59 actinobacillus pleuropneumoniae strains and 26 mycoplasma hyopneumoniae strains isolated from the respiratory tract of pigs. an additional set of 76 p multocida strains isolated from pneumonic pigs was tested for their minimum inhibitory concentrations of doxycycline. the p multocida and a pleuropneumoniae strains were isolated in france and the minimum inhibitory con ... | 1997 | 9253829 |
| swine mycoplasmoses. | mycoplasma hyopneumoniae is the primary agent of enzootic pneumonia in pigs. the lung lesions, generally observed in young pigs, are characterised by a hyperplasia of the epithelial cells and an increased perivascular and peribronchiolar accumulation of mononuclear cells. following m. hyopneumoniae infection, immune reactions are observed and resistance is induced in pigs. laboratory diagnosis is generally performed by an immunofluorescent test and by enzyme-linked immunosorbent assay. antibioti ... | 1996 | 9190026 |
| oral immunization of mice with attenuated salmonella typhimurium aroa expressing a recombinant mycoplasma hyopneumoniae antigen (nrdf). | mycoplasma hyopneumoniae is the etiological agent of porcine enzootic pneumonia, a commercially expensive respiratory disease of swine. salmonella typhimurium sl3261 was used as a live carrier of plasmid pkf1, which encodes a 15-kda recombinant m. hyopneumoniae protein. this expressed recombinant protein consists of the carboxy-terminal 11 kda of a 42-kda m. hyopneumoniae nrdf ribonucleotide reductase r2 subunit protein. rabbit anti-15-kda serum was able to inhibit the growth of viable m. hyopne ... | 1997 | 9169801 |
| molecular cloning and analysis of a hsp (heat shock protein)-like 42 kda antigen gene of mycoplasma hyopneumoniae. | the recombinant clone expressing the 42 kda protein (p42) of mycoplasma hyopneumoniae in escherichia coli was analyzed. the 4.4 kb hindiii-xmal dna fragment expressing the p42 gene product encodes three orfs: p42 and p16 in the forwarding strand, p24 in the reverse strand. sequence comparisons revealed that p42 could be part of a p65 gene, and has 62% identities with mycoplasma genitalium hsp70 gene and 56% identities with bacillus subtilis dnak gene; p16 and p24 genes share 73% and 47% identiti ... | 1997 | 9111943 |
| mycoplasma hyopneumoniae infection in pigs: duration of the disease and evaluation of four diagnostic assays. | 200 spf pigs were infected by aerosol with mycoplasma hyopneumoniae and the development of clinical signs, serological and pathological reactions were studied. mean time to onset of coughing was 13 days. a mean delay of 9 days was observed from onset of coughing until seroconversion against m. hyopneumoniae as measured by elisa. at an individual level, the sensitivity for this elisa was estimated to 98-100% and the specificity to 93-100%. pasteurella multocida was isolated from the majority of t ... | 1997 | 9050168 |
| identification of novel species-specific antigens of mycoplasma hyopneumoniae by preparative sds-page elisa profiling. | mycoplasma hyopneumoniae, m. hyorhinis and m. flocculare are commonly isolated from the respiratory tract of pigs and are phylogenetically related. the identification and characterization of antigens specific for m. hyopneumoniae is crucial for the development of serological reagents and for understanding the mechanisms of pathogenicity of this pathogen. protein and antigen profiles of six strains of m. hyopneumoniae, four strains of m. hyorhinis and a type strain of m. flocculare were compared ... | 1997 | 9043142 |
| characterization of the gene encoding mhp1 from mycoplasma hyopneumoniae and examination of mhp1's vaccine potential. | the gene encoding mhp1, a 124 kda protein from mycoplasma hyopneumoniae, has been cloned, sequenced, and its product characterized. no significant homology to the gene or encoded polypeptide was found in the genbank, nbrf, or pir databases, though this protein appears similar to p97, a putative adhesin of m. hyopneumoniae described by zhang et al. (infect. immun. 63, 1013-1019, 1995). two repeated motifs were identified within the 3' end of the gene and encoded polypeptide. the mhp1 gene was fus ... | 1997 | 9041663 |
| cloning and functional analysis of the p97 swine cilium adhesin gene of mycoplasma hyopneumoniae. | colonization of the swine respiratory tract by mycoplasma hyopneumoniae is accomplished by specific binding to the cilia of the mucosal epithelial cells. previous studies have implicated a 97-kda outer membrane-associated protein, p97, that appeared to mediate this interaction. in order to further define the role of p97 in adherence to porcine cilia, the structural gene was cloned and sequenced, and the recombinant products were analyzed. monoclonal antibodies were used to identify recombinant c ... | 1997 | 9023217 |
| economic evaluation of national eradication and control strategies for mycoplasma hyopneumoniae in denmark. | 1996 | 8996888 | |
| restricted b-cell responses to microbial challenge of the respiratory tract. | the local b-cell response in the respiratory tract to infectious challenge has been analyzed in pigs and calves using two techniques: flow cytometry and antibody secreting cell (asc) probes. pneumonia in pigs caused by experimental infection with mycoplasma hyopneumoniae resulted in a 25-fold increase in the b-cell population in bal and lung parenchyma 28 days post infection. asc probes revealed that the b-cell response of immune pigs to a large challenge infection was localized to lung parenchy ... | 1996 | 8988866 |
| human prostatic carcinoma oncogene pti-1 is expressed in human tumor cell lines and prostate carcinoma patient blood samples. | rapid expression cloning and differential rna display identifies a gene, named prostate tumor inducing gene-1 (pti-1), that is differentially expressed in prostate cancer versus normal prostate and benign prostatic hypertrophy. pti-1 encodes a truncated and mutated human elongation factor 1 alpha, and its 5' untranslated region (utr) shares significant homology with the 23s rrna gene of mycoplasma hyopneumoniae. pcr with human genomic dnas, using pti-1 5' utr-specific primers, suggests that this ... | 1997 | 8988032 |
| structure and evolution of ribonuclease p rna in gram-positive bacteria. | the sequences and structures of rnase p rnas of some gram-positive bacteria, e.g. bacillus subtilis, are very different than those of other bacteria. in order to expand our understanding of the structure and evolution of rnase p rna in gram-positive bacteria, gene sequences encoding rnase p rnas from 10 additional species from this evolutionary group have been determined, doubling the number of sequences available for comparative analysis. the enlarged data set allows refinement of the secondary ... | 1996 | 8972865 |
| enzootic pneumonia in pigs. | this article reviews current knowledge concerning enzootic pneumonia. enzootic pneumonia, caused by mycoplasma hyopneumoniae and exacerbated by secondary invaders, occurs worldwide and has been known for many years. the organism, with its typical characteristics, invades the respiratory tract in several successive steps. clinical symptoms and lesion development are the result of the pathogenic capacity of mycoplasma hyopneumoniae and the defence reactions in the lung. the economic relevance of p ... | 1996 | 8903144 |
| suppressive effect of bronchoalveolar lavage fluid from pigs infected with mycoplasma hyopneumoniae on chemiluminescence of porcine peripheral neutrophils. | bronchoalveolar lavage fluid (balf) from pigs experimentally infected with mycoplasma hyopneumoniae suppressed the chemiluminescence (cl) response of porcine polymorphonuclear neutrophils (pmn). the suppressive effect was significantly correlated with the concentration of prostaglandin e2 (pge2) in the balf. furthermore, purified human pge2 suppressed the cl response of porcine pmn. the increased level of pge2 following infection with m. hyopneumoniae may be responsible for the suppression of pm ... | 1996 | 8792569 |
| [comparison of the use of a vaccine and sequential antibiotic treatment in a herd infected with mycoplasma hyopneumoniae]. | the purpose of this study was to compare the efficacy of mycoplasma hyopneumoniae vaccination with pulse medication in a pig herd chronically infected with mycoplasma hyopneumoniae. control groups of pigs were compared to vaccinated or treated groups. medication and vaccination induced a significant reduction of pneumonia score (46.37% and 29.8% respectively, compared to 53.85% for control groups, p < 0.05). the treatment did not induce a significant reduction of the mean range of lung lesions ( ... | 1996 | 8767886 |
| analysis of putative abc transporter genes in mycoplasma hyopneumoniae. | a previously described dna probe specific for mycoplasma hyopneumoniae (i-141) was fully sequenced and found to consist of 1618 bp and to contain two tandemly repeated orfs. the deduced amino acid sequence of the two orfs showed significant homologies with atp-binding cassette (abc) transporter proteins, particularly those of the eukaryotic multidrug resistance (mdr) protein family (up to 21% identity and 47% similarity). a somewhat lower homology was evident with the secretion protein hlyb of t ... | 1996 | 8757749 |
| porcine reproductive and respiratory syndrome virus does not exacerbate mycoplasma hyopneumoniae infection in young pigs. | the purpose of this study was to determine if porcine reproductive and respiratory syndrome (prrs) virus infection altered the severity of acute mycoplasma hyopneumoniae (mh) infection in young pigs. twenty five, 3-week-old male pigs were randomly assigned by litter and weight to one of 3 groups. groups 1 (prrs only, n = 5) and 2 (prrs + mh, n = 10) were inoculated intranasally with prrs virus (in-5 isolate, 10(5) tcid50) and viremia in all pigs was confirmed by virus isolation from serum 3 days ... | 1996 | 8734647 |
| a field trial to evaluate a mycoplasma hyopneumoniae vaccine: effects on lung lesions and growth rates in swine. | a killed mycoplasma hyopneumoniae vaccine was evaluated in a single swine herd in which the farrowing barn and weaner rooms were on one mycoplasma-free farm, while the growing and finishing barn was on a separate farm on which mycoplasma was present. the study was carried out in a cohort of pigs born in a 12-week period. pigs born in 6 of the 12 wk were vaccinated and the rest were left as controls. the vaccine was administered twice at approximately 3 and 6 wk of age. carcass characteristics, l ... | 1996 | 8705975 |
| polymerase chain reaction for mycoplasma hyopneumoniae detection in tracheobronchiolar washings from pigs. | we have used the polymerase chain reaction (pcr) to detect mycoplasma hyopneumoniae in tracheobronchiolar washings collected from experimentally infected piglets. on the basis of the published nucleotide sequence of m. hyopneumoniae i141 probe (accession number u02537), primers were chosen to produce an amplified fragment of 1561 bp. all the m. hyopneumoniae strains tested could be detected by the pcr test. dna from other mycoplasmal and bacterial species currently isolated from respiratory trac ... | 1996 | 8684372 |
| purification of hydrophobic integral membrane proteins from mycoplasma hyopneumoniae by reversed-phase high-performance liquid chromatography. | a general and practical approach for isolating, fractionating and purifying large quantities of outer membrane hydrophobic proteins is described as applied to membrane proteins of mycoplasma hyopneumoniae. outer membrane proteins were extracted with triton x-114 detergent and were precipitated from the detergent phase with 90% ethanol. precipitated proteins were dissolved in 65% formic acid and separated by rp-hplc using a formic acid-acetonitrile gradient. a m(r) 48 000 protein was obtained in ... | 1996 | 8673256 |
| phylogenetic comparative mutational analysis of the base-pairing between rnase p rna and its substrate. | we have studied the base-pairing between the 3'-terminal cca motif of a trna precursor and rnase p rna by a phylogenetic mutational comparative approach. thus, various derivatives of the escherichia coli trna(ser)su1 precursor harboring all possible substitutions at either the first or the second c of the 3'-terminal cca motif were generated. cleavage site selection on these precursors was studied using mutant variants of m1 rna, the catalytic subunit of e. coli rnase p, carrying changes at posi ... | 1996 | 8665413 |
| dietary polyunsaturated fatty acids modulate responses of pigs to mycoplasma hyopneumoniae infection. | polyunsaturated fatty acids (pufa) are immunomodulators, but few studies have examined how these dietary components influence infectious respiratory disease. groups of nine pigs were fed casein and corn starch-based diets containing 10.5 g/100 g corn oil (co), linseed oil (lo), menhaden oil (mo), linseed + corn oil (lc, 1:1) and menhaden + corn oil (mc, 1:1). as a methodological control, one group of pigs (n = 15) was fed a commercial ration (control diet; c). pigs inoculated intratracheally wit ... | 1996 | 8648426 |
| molecular characterization of a ribonucleotide reductase (nrdf) gene fragment of mycoplasma hyopneumoniae and assessment of the recombinant product as an experimental vaccine for enzootic pneumonia. | a mycoplasma hyopneumoniae clone bank was screened with hyperimmune pig serum. one clone exhibited sequence homology to the prokaryotic r2 subunit of ribonucleotide reductase and was expressed as an 11-kda protein fused to beta-galactosidase. the vaccine potential of the fusion protein was assessed in pig trials. following experimental challenge with a virulent isolate of m. hyopneumoniae, gross lung pathology (mean goodwin lung score) of vaccinated animals, irrespective of adjuvant treatment, w ... | 1996 | 8641761 |
| growth and microbial flora of nonmedicated, segregated, early weaned pigs from a commercial swine operation. | to determine whether segregated, early weaned pigs have better growth performance and different microbial flora than those pigs raised on-site. | 1996 | 8617629 |
| arbitrarily primed pcr analysis of mycoplasma hyopneumoniae field isolates demonstrates genetic heterogeneity. | mycoplasma hyopneumoniae is the primary agent of mycoplasmal pneumonia in swine. in this study we performed an arbitrarily primed pcr (ap-pcr) analysis, in which low-stringency amplification with a single primer was used, to investigate genetic variability in m. hyopneumoniae strains and field isolates. we performed preliminary experiments to examine the efficacy of 40 different 10-mer oligonucleotides for priming an ap-pcr with m. hyopneumoniae jt (t = type strain) chromosomal dna. on the basis ... | 1996 | 8573513 |
| the influence of age and health status on the serum alpha 1-acid glycoprotein level of conventional and specific pathogen-free pigs. | serum alpha-1-acid glycoprotein (alpha 1ag) was measured in 212 landrace white pigs between birth and finishing age. the alpha 1ag level of healthy pigs five to ten months of age was 338 +/- 79 micrograms/ml, and the upper normal limit in mature swine has been established as 500 micrograms/ml. in both specific pathogen-free (spf) and conventional pigs, the alpha 1ag level within one day of birth was 14,263 +/- 2,393 micrograms/ml, 40 times the normal adult value, but rapidly decreased to 699 +/- ... | 1993 | 8490810 |
| incidence of infections in pigs bred for slaughter revealed by elevated serum levels of interferon and development of antibodies to mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae. | pigs in three specialized fattening herds were studied with respect to some immune functions, viral infections revealed by presence of interferon-alpha in serum as well as bacterial infections indicated by antibodies to mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae. pigs from different breeders had signs of varying but low incidence of such infections. after transport to the fattening herds, viral infections were activated and spread during the first month, and a maximum of 19% of ... | 1993 | 8456565 |
| seroepidemiology of mycoplasmal pneumonia of swine in japan as surveyed by an enzyme-linked immunosorbent assay. | antibodies to mycoplasma hyopneumoniae were surveyed by enzyme-linked immunosorbent assay (elisa) on serum, colostrum and milk samples collected from sows and on sera of growing/finishing pigs in japan. only one of 196 specific-pathogen-free sows induced a low elisa value, while 72% of 411 sows from conventional herds were seropositive. a seropositive rate in the conventional sows gradually decreased with an increase in farrowing frequency or with age. in 3267 growing/finishing pigs ranging in a ... | 1993 | 8451831 |
| application of enzyme-linked immunosorbent assay for the surveillance of mycoplasma hyopneumoniae infection in pigs. | a monoclonal antibody-based blocking enzyme-linked immunosorbent assay (elisa) was used for serological surveillance of mycoplasma hyopneumoniae infection in pig herds. a follow-up study was conducted on "herd predictive values" previously reported for this elisa. of those herds giving positive results by this elisa, 42% were subsequently found to be infected, while 100% of herds giving negative results were uninfected. previous reports recorded positive and negative herd predictive values of 39 ... | 1993 | 8400395 |
| control of infections with mycoplasma hyopneumoniae in swine herds by disrupting the chain of infection, disinfection of buildings and strategic medical treatment. | a program with the aim to eliminate infections with mycoplasma hyopneumoniae was applied in 4 herds selling breeding pigs. a "fast" (1 month) variant of the program was applied in one herd selling young gilts (30 kg body weight) and in one herd selling adult breeding stock, and a "slow" (> or = 3.5 months) variant was applied in two corresponding herds. in both variants, the spread of infections was prevented by isolating the breeding stock. these animals were treated with tiamulin before being ... | 1993 | 8342364 |
| mycoplasma hyopneumoniae infection in pigs: duration of the disease and resistance to reinfection. | forty-eight hysterectomy produced piglets reared in a pathogen-free environment were housed in 4 separate units. three groups were intratracheally challenged with m hyopneumoniae at 2 wk, or at 16 wk, or at 2 and 16 wk of age. the fourth group was uninfected and received broth medium. coughing started 2 weeks after the first infection but the booster infection did not induce any coughing. macroscopic lesions, typical of mycoplasmal pneumonia, were noted 1 wk post-infection. after 7-9 wk, piglets ... | 1993 | 8319010 |
| aerosol vaccination of pigs against mycoplasma hyopneumoniae infection. | aerosol vaccination is used effectively to immunize poultry against newcastle disease, but to the authors' knowledge, this vaccination procedure is not well studied in other species. the efficacy of im and aerosol vaccination of pigs against mycoplasma hyopneumoniae infection was evaluated. twenty-one pigs from a mycoplasma-free herd were randomly allotted by litter and body weight into 3 groups. one group was given aerosolized phosphate-buffered saline solution (pbss) by inhalation. the second ... | 1993 | 8291766 |
| increased levels of tumor necrosis factor and interleukin 1 in bronchoalveolar lavage fluids from pigs infected with mycoplasma hyopneumoniae. | we examined the levels of tumor necrosis factor (tnf)-alpha and interleukin-1 (il-1) in bronchoalveolar lavage fluids (balf) from pigs experimentally infected with mycoplasma hyopneumoniae using biological assays with wehi-164 cells and d10.g4.1 cells, respectively. increased tnf-alpha and il-1 in balf were found in infected pigs with gross and/or microscopic lesions. a time-course study suggested tnf-alpha and il-1 to be persistently elevated in infected pigs. their presence in balf would thus ... | 1993 | 8291203 |