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phage formation in staphylococcus muscae cultures. x. the relationship between virus synthesis, the release of bacterial ribonucleic acid, virus liberation, and cellular lysis.1. under a variety of conditions in which cells are infected with one or a few virus particles and the host cells are killed, but no infective particles or virus material is formed as indicated by plaque count, one-step growth curve, or protein or desoxyribonucleic determinations, the cells neither lyse nor release ribonucleic acid into the medium. 2. the "killing" effect of s. muscae phage is separate from its lytic property. 3. the release of ribonucleic acid into the medium is not simply due ...195214898025
two distinct pathways for the formation of hydroxy fa from linoleic acid by lactic acid bacteria.twenty-three of 86 strains of lactic acid bacteria transformed linoleic acid into hydroxy fa. two distinct conversion pathways were in operation. two strains of lactobacillus acidophilus and a strain of pediococcus pentosaceus produced 13(s)-hydroxy-9-octadecenoic acid 113(s)-oh 18:11 and 10,13dihydroxyoctadecanoic acid (10,13-oh 18:0) as main and minor products, respectively, whereas 13 strains, including l. casei subsp. casei, l. paracasei subsp. paracasei, l. rhamnosus, l. lactis subsp. cremo ...200314870930
molecular characterization of lactic acid populations associated with wine spoilage.we have investigated the prevalence of spoilage lactic acid bacteria (lab) in table wines produced in the apulia region. the occurrence of lab was evaluated in wines produced with low sulphur dioxide doses and not supplemented with selected malolactic starters such as oenococcus oeni. about 150 strains were isolated from wine must and a molecular characterization was performed using pcr-based techniques. most of the strains analysed belonged to lactobacillus plantarum species. however, some of t ...200414768022
survival of lactic acid bacteria in seawater: a factorial study.a feasibility study of lactic bacteria as potential probiotics in larval cultures of marine fish was performed by investigating the survival of five strains of lactic bacteria in seawater by readily standardized procedures at different temperatures and salinities. these conditions were chosen in such a way that their combinations define a complete first-order factorial design. depending on the strain and the ambient conditions, the survival adhered to first-order kinetics in some cases, and to t ...200314756536
a rapid turbidometric microplate bioassay for accurate quantification of lactic acid bacteria bacteriocins.a 1 day turbidometric microplate bioassay (tmb) was developed for the rapid, accurate and precise quantification of lactic acid bacteria (lab) bacteriocins (nisin z and pediocin pa-1). parameters such as the concentration of the indicator strains and the incubation time were optimized for each bacteriocin. a high correlation coefficient (r(2)=0.992+/-0.004) was obtained for the exponential regression in the nisin z concentration range of 20-120 ng/ml with 1 x 10(7) cfu indicator strain (pediococ ...200414751683
observations on the succession dynamics of lactic acid bacteria populations in chill-stored vacuum-packaged beef.drip samples were collected at 4-week intervals from 10 vacuum-packaged beef striploins stored for 16 weeks at -1.5 degrees c and assayed for populations of lactic-acid bacteria (lab), ph and spoilage-causing fermentation products. a total of 15 lab species were identified using pulsed-field gel electrophoresis and biochemical analysis. a pattern of succession was observed during storage between strains of carnobacterium, lactobacillus, leuconostoc and pediococcus. acetic acid production was ass ...200414751682
microbiological characterization and probiotic potential of koko and koko sour water, african spontaneously fermented millet porridge and drink.to identify and examine the diversity of predominant lactic acid bacteria (lab) in koko and koko sour water (ksw) from different ghanaian production sites with regard to pattern of fermentation (api 50 chl), genotype, antimicrobial activity, and resistance to low ph and bile salts.200414723700
generation and utilization of polyclonal antibodies to a synthetic c-terminal amino acid fragment of divercin v41, a class iia bacteriocin.polyclonal antibodies have been generated by immunization of rabbits with a chemically synthesized c-terminal part of divercin v41 (dvnct) conjugated to the carrier protein keyhole limpet hemocyanin (klh). the sensitivity and reactivity of the dvnct-klh-generated antibodies were evaluated by enzyme-linked immunosorbent assay (elisa) using supernatant from cultures of 13 representative lactic acid bacterium strains, and specificity was confirmed by western blot analysis. anti-dvnct-klh antibodies ...200414711648
identification of carnobacterium, lactobacillus, leuconostoc and pediococcus by rdna-based techniques.ribosomal dna-based techniques including the analysis of profiles generated by isr amplification, isr restriction and ardra have been evaluated as molecular tools for identifying carnobacterium, lactobacillus, leuconostoc and pediococcus. they have been applied for the molecular characterization of 91 strains with the following identities: eight carnobacterium including the eight type species of the genus; 61 lactobacillus including 40 type strains out of 45 species, 13 leuconostoc, out of them ...200314666983
production of plantaricin nc8 by lactobacillus plantarum nc8 is induced in the presence of different types of gram-positive bacteria.lactobacillus plantarum nc8 was shown to produce plantaricin nc8 (plnc8), a recently purified and genetically characterized inducible class iib bacteriocin, only when it was co-cultured with other gram-positive bacteria. among 82 strains belonging to the genera bacillus, enterococcus, lactobacillus, lactococcus, leuconostoc, listeria, pediococcus, staphylococcus, and streptococcus, 41 were shown to induce plnc8 production in l. plantarum nc8. there was apparently no relationship between the sens ...200414647979
substrate inhibition of pediococcus acidilactici by glucose on a waste medium. simulations and experimental results.the possibility of substrate inhibition by glucose on biomass and pediocin production was studied in cultures of pediococcus acidilactici on a residual medium.200314633105
beer spoilage bacteria and hop resistance.for brewing industry, beer spoilage bacteria have been problematic for centuries. they include some lactic acid bacteria such as lactobacillus brevis, lactobacillus lindneri and pediococcus damnosus, and some gram-negative bacteria such as pectinatus cerevisiiphilus, pectinatus frisingensis and megasphaera cerevisiae. they can spoil beer by turbidity, acidity and the production of unfavorable smell such as diacetyl or hydrogen sulfide. for the microbiological control, many advanced biotechnologi ...200314623377
the effects of using lactic acid bacteria inoculants in maize silage on the formation of biogenic amines.silages from five ripened varieties of silage maize with dry matter contents ranging between 275 and 410 g/kg were prepared in five laboratory experiments. whole-plant maize was fermented at 22 degrees c and silages were then stored at the same temperature for 4 months. spontaneously fermented silages were prepared as control variants and compared with silages inoculated with commercial strains of lactobacillus plantarum, lactobzcillus buchneri and a mixed preparation microsil containing l. plan ...200314620909
construction and use of a computerized dna fingerprint database for lactic acid bacteria from silage.efficient selection of new silage inoculant strains from a collection of over 10,000 isolates of lactic acid bacteria (lab) requires excellent strain discrimination. toward that end, we constructed a gelcompar ii database of dna fingerprint patterns of ethidium bromide-stained ecori fragments of total lab dna separated by conventional agarose gel electrophoresis. we found that the total dna patterns were strain-specific; 56/60 american type culture collection strains of 33 species of lab could b ...200314607400
islpl1 is a functional is30-related insertion element in lactobacillus plantarum that is also found in other lactic acid bacteria.we describe the first functional insertion sequence (is) element in lactobacillus plantarum. islpl1, an is30-related element, was found on the plp3 plasmid in strain fb335. by selection of spontaneous mutants able to grow in the presence of uracil, it was demonstrated that the is had transposed into the uracil phosphoribosyltransferase-encoding gene upp on the fb335 chromosome. the plasmid-carried is element was also sequenced, and a second potential is element was found: islpl2, an is150-relate ...200314532059
16s-ardra, a tool for identification of lactic acid bacteria isolated from grape must and wine.lactic acid bacteria (lab) are found in a great variety of habitats, including grape must and wines. there is a close relationship between the species of lab which develop during fermentation and the eventual quality of the wine. for these reasons analytical techniques allowing fast and reliable identification of wine lab are needed. in this work a simple and accurate protocol for identifying species of lab isolated from grape must and wine is presented. this protocol is based on the amplificati ...200314529184
cloning, sequence analysis and expression of the f1f0-atpase beta-subunit from wine lactic acid bacteria.the nucleotide sequences of the genes encoding the f1f0-atpase beta-subunit from oenococcus oeni, leuconostoc mesenteroides subsp. mesenteroides, pediococcus damnosus, pediococcus parvulus, lactobacillus brevis and lactobacillus hilgardii were determined. their deduced amino acid sequences showed homology values of 79-98%. data from the alignment and atpase tree indicated that o. oeni and l. mesenteroides subsp. mesenteroides formed a group well-separated from p. damnosus and p. parvulus and fro ...200314529177
enumeration of probiotic pediococci in animal feed: interlaboratory study.an enumeration method to be used as official control under council directive 70/524/eec for probiotic pediococci used as feed additives was validated for consideration for adoption as comitée européen de normalisation (cen) and iso standards. seventeen laboratories in 11 european countries carried out an interlaboratory study. a spread plate method following bs iso 15214:1998 using 4 different agars [mrs, acidified mrs, mrs with triphenyl tetrazolium chloride (ttc), and a newly developed pedioco ...200314509440
evaluation of several modifications of an ecometric technique for assessment of media performance.recovery of listeria monocytogenes 101m, jonesia denitrificans, salmonellae, and pediococcus sp. nrrl b-2354 across nine media was evaluated with three modified versions of an ecometric method. two approaches involved the use of broth cultures (10(8) to 10(9) cfu/ml) of individual strains and either large (10-microl) or small (1-microl) presterilized plastic loops. the third approach involved precultured slants and the inoculation of media with presterilized plastic inoculating needles (10(4) cf ...200314503736
folic acid derivatives in yeast. 196514342346
relationships between biotin and vitamin b12. effects of biotin and vitamin b12 on folic acid metabolism.1. the effects of dietary biotin compared with vitamin b(12) on the total content and on the distribution of the various folate derivatives in the liver of rats given a biotin-free diet have been studied. the effect of both vitamins on the conversion in vitro of folic acid into citrovorum factor in the same experimental conditions was also examined. 2. in biotin-treated rats as well as in vitamin b(12)-treated rats the total content of folic acid-active substances measured microbiologically by p ...196514340069
silage bacteriology. ii. influence of temperature differences on the composition of the lactic microflora. 196514333082
beer-spoilage bacteria and their control with a phosphoric acid--ammonium persulfate wash. 196514332530
the fate of orally and parenterally administered folates. 196514332350
distribution and characteristics of the catalases of lactobacillaceae.johnston, m. a. (cornell university, ithaca, n.y.), and e. a. delwiche. distribution and characteristics of the catalases of lactobacillaceae. j. bacteriol. 90:347-351. 1965.-certain strains of lactobacilli and pediococci incorporated hematin during growth, with the concomitant formation of cyanide- and azide-sensitive catalase. three of five strains of lactobacilli and five of 25 strains of pediococci were capable of this biosynthesis. the pediococci required the heme component of blood, wherea ...196514329446
role of leuconostoc mesenteroides in leavening the batter of idli, a fermented food of india.the fermentation of the batter of idli, a fermented food of india, was studied. the microorganisms responsible for the characteristic changes in the batter were isolated and identified. although there is a sequential change in the bacterial flora, the predominant microorganism responsible for souring, as well as for gas production, was found to be leuconostoc mesenteroides. in the later stages of fermentation, growth of streptococcus faecalis and, still later, of pediococcus cerevisiae becomes s ...196514325884
[disturbance in folic acid metabolism in vitamin b12 deficiency induced by anti-vitamin b12]. 196414251719
comparative bioautography of folic and folinic acids of erythrocytes and livers of normal ducks and ducks infected with malarial parasites. 196414244807
medium for producing cells of lactic acid bacteria. 196414239585
alterations in circulating folate induced by b12 deficiency. 196414233476
some characteristics of an oleate-requiring, hemolytic pediococcus.deibel, r. h. (st. james hospital, chicago heights, ill.), j. h. silliker, and p. t. fagan. some characteristics of an oleate-requiring, hemolytic pediococcus. j. bacteriol. 88:1078-1083. 1964.-a gram-positive, catalase-negative, tetradforming coccus, isolated from a bronchial aspirate, is described. the organism was classified as a member of the genus pediococcus on the basis of morphological and physiological characteristics. it differed from previously described members of this genus in that ...196414219022
further studies of the genus pediococcus. 196414215438
properties of reduced derivatives of aminopterin. 196414213374
blood folic acid studies. vii. purification and properties of the folic acid precursors of human erythrocytes. 196414209970
new fluorometric method for estimation of citrovorum factor. 196414186667
synthesis and antibacterial properties of some tripeptides. 196314184939
meta- and ortho-(n-bis(2-chloroethyl))-aminobenzoylglutamic acids. 196414151990
preparation and growth-inhibitory properties of new polycyclic 2,4-diaminopyrimidines. 196414151862
[on the polyols formed in lactic fermentation of glucides]. 196414146827
substitution of manganese for tomato juice in the cultivation of lactic acid bacteria.tomato juice was separated by chemical and physical methods into various active fractions, as measured by growth response and acid production by numerous lactic acid bacteria. incineration of the treated extracts with little apparent loss in activity established the fact that the stimulatory component was of inorganic composition. of the various cations tested, manganese was the only element that produced biological activity comparable to that of the original extract. of the 71 strains of lactic ...196414133240
the biosynthesis of folic acid. iii. enzymatic formation of dihydrofolic acid from dihydropteroic acid and of tetrahydropteroylpolyglutamic acid compounds from tetrahydrofolic acid. 196414114858
the pteroylglutamate components of american diets as determined by chromatographic fractionation. 196314086780
[study of a bacterium isolated from cheese source of vitamin b12 and other group b vitamins]. 196314081747
enzyme formation in pediococcus pentosaceus treated with mitomycin c. 196314066495
the use of soft agar in the study of conditions affecting the utilization of fermentable substrates by lactic acid bacteria. 196314058987
glucose dehydrogenase activity in pediococcus pentosaceus. 196314051811
pentose utilization by pediococcus pentosaceus.dobrogosz, walter j. (university of illinois, urbana) and ralph d. demoss. pentose utilization by pediococcus pentosaceus. j. bacteriol. 85:1356-1364. 1963.-data are presented which indicate that pentoses are metabolized by pediococcus pentosaceus through the use of an inducible phosphoketolase pathway. the utilization of each of the pentoses (l-arabinose, d-ribose, and d-xylose) appears to involve a different regulatory process. thus, d-ribose was fermented by cells grown on any pentose or gluc ...196314047230
induction and repression of l-arabinose isomerase in pediococcus pentosaceus.dobrogosz, walter j. (university of illinois, urbana) and ralph d. demoss. induction and repression of l-arabinose isomerase in pediococcus pentosaceus. j. bacteriol. 85:1350-1355. 1963.-the inducible l-arabinose isomerase of pediococcus pentosaceus can be rapidly and conveniently measured in whole-cell preparations by use of a standard colorimetric procedure originally developed for studies with cell-free enzyme preparations. the enzyme is measured by its ability to catalyze the isomerization o ...196314047229
folic acid derivatives synthesized during growth of diplococcus pneumonia.sirotnak, f. m. (sloan-kettering institute for cancer research, new york, n.y.), gloria j. donati, and dorris j. hutchison. folic acid derivatives synthesized during growth of diplococcus pneumoniae. j. bacteriol. 85:658-665. 1963.-under cultural conditions permitting synthesis of folic acid in an amount greatly in excess (20- to 30-fold) of that required for maximal growth of diplococcus pneumoniae, 85 to 90% of the growth factor accumulated as polyglutamates. approximately equal amounts of mon ...196314046462
nature of the lipids of some lactic acid bacteria.ikawa, miyoshi (university of california, berkeley). nature of the lipids of some lactic acid bacteria. j. bacteriol. 85:772-781. 1963.-lactobacillus casei, l. plantarum, leuconostoc mesenteroides, pediococcus cerevisiae, and streptococcus faecalis were grown on a lipid-free medium, and the amounts of acetone-soluble lipids, acetone-insoluble phospholipids, and unextracted or bound lipid in the cells were estimated. neutral glycerides appeared to be absent, or present only in traces, in l. casei ...196314044942
interrelationship between temperature and sodium chloride on growth of lactic acid bacteria isolated from meat-curing brines.goldman, manuel (american meat institute foundation, chicago, ill.), r. h. deibel, and c. f. niven, jr. interrelationship between temperature and sodium chloride on growth of lactic acid bacteria isolated from meat-curing brines. j. bacteriol. 85:1017-1021. 1963.-an elevation of the temperature limit for growth of some pediococcus homari (gaffkya homari) and motile lactobacillus strains could be effected by the addition of sodium chloride to the growth medium. at the optimal temperature for grow ...196314043988
oxidative metabolism in pediococcus pentosaceus. ii. factors controlling the formation of oxidative activities.dobrogosz, walter j. (the pennsylvania state university, university park) and robert w. stone. oxidative metabolism in pediococcus pentosaceus. ii. factors controlling the formation of oxidative activities. j. bacteriol. 84:724-729. 1962.-studies were conducted on some factors which regulate the formation of oxidative activities in pediococcus pentosaceus, a homofermentative lactic acid organism. oxidative abilities were defined as the capacities to oxidize glucose and glycerol, and to exhibit c ...196214028240
oxidative metabolism in pediococcus pentosaceus. i. role of oxygen and catalase.dobrogosz, walter j. (pennsylvania state university, university park) and robert w. stone. oxidative metabolism in pediococcus pentosaceus. i. role of oxygen and catalase. j. bacteriol. 84:716-723. 1962.-studies were conducted on the physiological behavior of several strains of pediococcus pentosaceus isolated from alfalfa silages. although these organisms are regarded as homofermentative lactic acid bacteria which metabolize carbohydrates via the classic reactions of glycolysis, this investigat ...196214028239
effects of uracil and thymidine on the development of resistance to 5-fluorouracil in pediococcus cerevisiae.white, p. j. (roswell park memorial institute, buffalo, n.y.) and c. a. nichol. effects of uracil and thymidine on the development of resistance to 5-fluorouracil in pediococcus cerevisiae. j. bacteriol. 85:97-105. 1963.-growth of pediococcus cerevisiae atcc 8081 in a basal semidefined medium was inhibited by 5-fluorouracil (fu) and 5-fluoro-2'-deoxyuridine (fudr). addition of uracil slightly decreased sensitivity to fu, but did not affect inhibition by fudr. addition of thymidine alone increase ...196314000344
a metabolic relationship of spermine to folinic acid and thymidine. 196313994914
3,4-dehydroproline, a proline antagonist. 196213989505
synthesis and antibacterial activity of n4-(l-valyl-l-valyl)-sulfanilamide and analogs. 196313976773
studies on the physiology of rickettsiae. iv. folic acids of coxiella burnetii.mattheis, martha s. (university of kansas, lawrence), m. silverman, and d. paretsky. studies on the physiology of rickettsiae. iv. folic acids of coxiella burnetii. j. bacteriol. 85:37-41. 1963.-yolk, yolk sac, and embryo tissues of uninfected eggs, and those infected with coxiella burnetii, were analyzed for folic acid derivatives by employing diethylaminoethyl (deae)-cellulose column chromatography. infected tissues contained quantitatively less folate, but the elution profiles of both infecte ...196313933852
the cultural and physiological characters of the pediococci. 196113902978
serological characters of the pediococci. 196113902977
the isolation, identification and synthesis of a peptide growth factor for p. cerevisiae. 196213893672
nature of the citrovorum factor requirement of pediococcus cerevisiae. 196013845832
comparative study of gaffkya homari, aerococcus viridans, tetrad-forming cocci from meat curing brines, and the genus pediococcus. 196013815447
catalase of pedicoccus cerevisiae. 196113721713
microbiology of meat curing. iv. a lyophilized pediococcus cerevisiae starter culture for fermented sausage. 196113721283
sparing of folinic acid by thymidine.thymidine, which is inactive when added alone to pediococcus cerevisiae (leuconostoc citrovorum) 8081, was found to increase its growth in the presence of folinic acid. the addition of thymidine increased both the sensitivity of the folinic acid assay and the total growth yield. the mechanism of the synergistic effect of thymidine is briefly discussed.196113709112
folinic acid requirment of strains of the genus pedicoccus. 196113703797
the effect of asparagine on the growth of a gram-positive coccus. 196113703796
reclassification of leuconostoc mesenteroides p. 60 as a pedicoccus. 195913657146
mode of division of pediococci. 195913644244
selection of bacterial mutants of increased sensitivity to aminopterin. 195913632794
catalase activity in pediococcus cerevisiae as related to hydrogen ion activity. 195713481014
the nutrition and physiology of the genus pediococcus. 195413152063
production of catalase by the pediococci. 195313069407
macrolide and lincosamide resistance in the gram-positive nasal and tonsillar flora of pigs.macrolide and lincosamide resistance phenotypes and the presence of the erm(a), erm(b), erm(c), and mef(a) genes were determined in 344 bacterial strains belonging to 34 species and nine genera, isolated from the tonsils and nasal cavities of 2-week- and 6-week-old piglets, derived from four different farms. these piglets had never before been treated with macrolides or lincosamides. macrolide and lincosamide resistance was most frequently present in streptococcus and enterococcus strains, of wh ...200312959408
exopolysaccharide production by pediococcus damnosus 2.6 in a semidefined medium under different growth conditions.ropy pediococcus damnosus (strain 2.6) was used for production of exopolysaccharide (eps) in a semidefined medium. from a kinetic point of view, an experiment conducted in smd medium containing 30 g l(-1) glucose and 5 g l(-1) bacto casamino acids (difco laboratories, detroit, mi), without ph control, showed that eps production took place mainly during the growth phase. the viscosity of the cultures developed in parallel to the eps synthesis until 94 h of incubation; after 200 h of fermentation, ...200312927713
development of specific fluorescent oligonucleotide probes for in situ identification of wine lactic acid bacteria.a rapid method for the identification of lactic acid bacteria (lab) from wine has been developed. this method is based on fluorescence in situ hybridisation (fish), using fluorescent oligonucleotide probes, homologous to 16s rdna of those species of lab commonly found in wines. the protocol for the specific detection of these bacteria was established through the hybridisation of 36 reference strains. the specificity of the probes was evaluated by using pure cultures. probes were used to identify ...200312900029
pediocin production by recombinant lactic acid bacteria.production of the anti-listerial bacteriocin, pediocin, by lactic acid bacteria (lab) transformed with the cloning vector ppc418 (ped+, 9.1 kb) was influenced by composition of media and incubation temperature. maximum pediocin production, tested against listeria innocua, by electrotransformants of lactococcus lactis ssp. lactis was measured in tryptone/lactose/yeast extract medium after 24 h growth at 30 degrees c, while incubation at 40 degrees c was optimum for ped+ transformants of streptoco ...200312882274
prevalence and diversity of tetracycline resistant lactic acid bacteria and their tet genes along the process line of fermented dry sausages.in order to study the prevalence and diversity of tetracycline resistant lactic acid bacteria (tc(r) lab) along the process line of two different fermented dry sausage (fds) types, samples from the raw meat, the meat batter and the fermented end product were analysed quantitatively and qualitatively by using a culture-dependent approach. both the diversity of the tet genes and their bacterial hosts in the different stages of fds production were determined. quantitative analysis showed that all r ...200312866855
influence of ph drop on both nisin and pediocin production by lactococcus lactis and pediococcus acidilactici.to develop a kinetic model for describing the specific effect of ph drop on nisin and pediocin production in whey.200312803556
potential osmoprotectants for the lactic acid bacteria pediococcus pentosaceus and tetragenococcus halophila.the physiological responses of the lactic acid bacteria pediococcus pentosaceus and tetragenococcus halophila (formely known as p. halophila), subjected to osmotic stress in the presence of molecules known to act as osmoprotectants for other bacteria were studied. in a defined medium, glycine betaine, dimethylsulfonioacetate, choline, proline and l-carnitine were able to relieve inhibition of growth at 0.8 m nacl. the five compounds were shown to efficiently compete with glycine betaine transpor ...200312781949
proteolytic activities in togwa, a tanzanian fermented food.proteolytic activities were investigated in sorghum-based togwa prepared by natural fermentation and using starter cultures previously isolated from the native product, i.e., lactobacillus brevis, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus plantarum, pediococcus pentosaceus, and issatchenkia orientalis in coculture with either l. brevis or l. plantarum. both proteinase and aminopeptidase activities were substantially higher in naturally fermented togwa than in those with s ...200312781948
use of starter cultures of lactic acid bacteria and yeasts in the preparation of togwa, a tanzanian fermented food.starter cultures of lactic acid bacteria (lactobacillus brevis, lactobacillus cellobiosus, lactobacillus fermentum, lactobacillus plantarum and pediococcus pentosaceus) and yeasts (candida pelliculosa, candida tropicalis, issatchenkia orientalis and saccharomyes cerevisiae) isolated from native togwa were tested singly or in combination for their ability to ferment maize-sorghum gruel to produce togwa. all species of bacteria showed an ability to ferment the gruel as judged by lowering the ph fr ...200312745235
bioprotectives and probiotics for dry sausages.the microbial stability of dry sausages is determined by the combination and timing of different factors referred to as the hurdle-concept. however, the hurdles present in dry sausage are not sufficient to prevent the survival of listeria monocytogenes or enterohemorrhagic escherichia coli o157:h7. recently bioprotective lactic acid bacteria, which in addition to the production of antimicrobial lactic acid, have been found to contribute to the safety of the dry sausage by producing antimicrobial ...200312745229
concomitant septic and gouty arthritis--an analysis of 30 cases.to analyse the clinical features and outcomes of gouty patients with concomitant septic arthritis in a medical centre.200312730521
conformational changes of pediocin in an aqueous medium monitored by fourier transform infrared spectroscopy: a biological implication.fourier transform infrared (ftir) spectroscopy was used to investigate the secondary structure of pediocin pa-1 in different aqueous media in relation to its antimicrobial activity. the experiments were performed at pd (ph meter corrected for deuterium isotope effect) 6, 7, and 8 and during a heating-cooling cycle of 20-80 degrees c. at pd 6, (i.e. pediocin's most active form), the ftir results show that pediocin adopts an unordered structure with a small contribution of beta-turn. after a heati ...200312719125
inhibition of nonproteolytic clostridium botulinum with lactic acid bacteria and their bacteriocins at refrigeration temperatures.nonproteolytic clostridium botulinum (strains 17b, beluga, and 202f) was found to be inhibited by lactobacillus, lactococcus, streptococcus, and pediococcus species in tests by the spot-on-the-lawn simultaneous-antagonism method at 10, 15, and 25 degrees c. c. botulinum 17b was the most resistant strain. inhibition zone size increased with decreasing incubation temperature. six strains of lactobacillus acidophilus and seven strains of bifidobacteria failed to produce an inhibition zone on buffer ...200312696695
bacteriological evaluation of dog and cat diets that claim to contain probiotics.nineteen commercial pet foods claiming to contain probiotics were evaluated. selective bacterial culture was performed to identify organisms that were claimed to be present. twelve diets claimed only to contain specific bacterial fermentation products, which does not necessarily indicate that live growth would be expected, but these products also included the term "probiotic" somewhere on the package, suggesting that live, beneficial organisms were present. no products contained all of the liste ...200312677689
functional replacement of the escherichia coli d-(-)-lactate dehydrogenase gene (ldha) with the l-(+)-lactate dehydrogenase gene (ldhl) from pediococcus acidilactici.the microbial production of l-(+)-lactic acid is rapidly expanding to allow increased production of polylactic acid (pla), a renewable, biodegradable plastic. the physical properties of pla can be tailored for specific applications by controlling the ratio of l-(+) and d-(-) isomers. for most uses of pla, the l-(+) isomer is more abundant. as an approach to reduce costs associated with biocatalysis (complex nutrients, antibiotics, aeration, product purification, and waste disposal), a recombinan ...200312676706
lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces.five strains of thermotolerant lactic acid bacteria (g 12, g 22, g 35t, g 43 and g 44) isolated from chicken faeces were characterized taxonomically. the strains were facultatively anaerobic, gram-positive, catalase-negative, non-motile, non-spore-forming rods. they were heterofermentative lactobacilli that produced dl-lactic acid. growth of the strains occurred at 45 degrees c but not at 15 degrees c. the optimum temperature for growth was 42 degrees c, as determined from the specific growth ra ...200312656183
isolation, selection, and characterization of lactic acid bacteria for a competitive exclusion product to reduce shedding of escherichia coli o157:h7 in cattle.lactic acid bacteria (lab) were selected on the basis of characteristics indicating that they would be good candidates for a competitive exclusion product (cep) that would inhibit escherichia coli o157:h7 in the intestinal tract of live cattle. fecal samples from cattle that were culture negative for e. coli o157:h7 were collected. lab were isolated from cattle feces by repeated plating on deman rogosa sharpe agar and lactobacillus selection agar. six hundred eighty-six pure colonies were isolat ...200312636285
autolytic activity and pediocin-induced lysis in pediococcus acidilactici and pediococcus pentosaceus strains.to evaluate the autolytic phenotype of pediococcus acidilactici and p. pentosaceus, the peptidoglycan hydrolases content and the effect of pediocin ach/pa-1 and autolysins on cell lysis.200312631191
strong synergy between a eukaryotic antimicrobial peptide and bacteriocins from lactic acid bacteria.the antimicrobial effect obtained upon combining the prokaryotic antimicrobial peptides (amps; more commonly referred to as bacteriocins) pediocin pa-1, sakacin p, and curvacin a (all produced by lactic acid bacteria [lab]) with the eukaryotic amp pleurocidin (from fish) has been investigated. the three lab amps alone were active against gram-positive listeria ivanovii bacteria at nanomolar concentrations, whereas they were inactive against gram-negative escherichia coli bacteria. pleurocidin al ...200312620872
broad and complex antifungal activity among environmental isolates of lactic acid bacteria.more than 1200 isolates of lactic acid bacteria isolated from different environments were screened for antifungal activity in a dual-culture agar plate assay. approximately 10% of the isolates showed inhibitory activity and 4% showed strong activity against the indicator mould aspergillus fumigatus. the antifungal spectra for 37 isolates with strong activity and five isolates with low or no activity were determined. several of the strains showed strong inhibitory activity against the moulds a. f ...200312594034
identification, isolation and quantification of representative bacteria from fermented cassava dough using an integrated approach of culture-dependent and culture-independent methods.the use of denaturing gradient gel electrophoresis (dgge) and traditional culture-depending methods for examining the bacterial community of traditional cassava starch fermentation were investigated. it appeared that dgge profiles of total dna of cassava dough exhibited 10 distinguishable bands. in contrast, dgge fingerprints of bacteria recovered from enrichment cultures of fermented dough gave variable profiles containing fewer bands. bands corresponding to five bacterial species detected by d ...200312568751
bacteriocins from pediococcus pentosaceus l and s from pork meat.two strains of pediococcus pentosaceus were isolated from refrigerated pork and found to produce antimicrobial substances that may inhibit foodborne pathogens and have potential as natural food preservatives. they were named p. pentosaceus l and s. the antimicrobial substances were purified to electrophoretical homogeneity by chloroform extraction and designated pentocins l and s with molecular masses (m) of 27 and 25 kda, respectively. both pentocins also had broad inhibition spectra and were t ...200312568574
[studies on the lactate oxidase producing conditions by edwardsiella tarda].a bacterium producing lactate oxidase was re-screened from five strains based on previous researches. the lactate oxidase activity was the highest in strain l1 and this stain was chosen as the enzyme source. morphological and physiological studies revealed that the bacterial strain l1 belongs to the edwardsiella tarda biogroup i. this stain is different from the reported strains mycobacterium and pediococcus, which produce lactate oxidase. the enzyme producing conditions were studied in shaking ...199912555418
the lactic acid bacteria: a literature survey.the purpose of this review article on the lactic acid bacteria grew from an early curiosity and a desire to convey and impart the broad scope of literary information on their functions as starter cultures, in the manufacture of fermentation products such as dairy products and alcoholic beverages, as well as their contribution to better health. this review article is an attempt to empower the reader and to circumvent the difficult task in acquiring and elucidating a large body of information. the ...200212546196
phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16s ribosomal dna analysis.a total of 161 low-g+c-content gram-positive bacteria isolated from whole-crop paddy rice silage were classified and subjected to phenotypic and genetic analyses. based on morphological and biochemical characters, these presumptive lactic acid bacterium (lab) isolates were divided into 10 groups that included members of the genera enterococcus, lactobacillus, lactococcus, leuconostoc, pediococcus, and weissella: analysis of the 16s ribosomal dna (rdna) was used to confirm the presence of the pre ...200312514026
purification and genetic characterization of plantaricin nc8, a novel coculture-inducible two-peptide bacteriocin from lactobacillus plantarum nc8.a new, coculture-inducible two-peptide bacteriocin named plantaricin nc8 (plnc8) was isolated from lactobacillus plantarum nc8 cultures which had been induced with lactococcus lactis mg1363 or pediococcus pentosaceus fbb63. this bacteriocin consists of two distinct peptides, named alpha and beta, which were separated by c(2)-c(18) reverse-phase chromatography and whose complementary action is necessary for full plantaricin nc8 activity. n-terminal sequencing of both purified peptides showed 28 a ...200312514019
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