Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| fluorescence in situ hybridization analysis of nitrifiers in piggery wastewater treatment reactors. | fluorescence in situ hybridization (fish) was performed to analyze the nitrifying microbial communities in an activated sludge reactor (asr) and a fixed biofilm reactor (fbr) for piggery wastewater treatment. heterotrophic oxidation and nitrification were occurring simultaneously in the asr and the cod and nitrification efficiencies depend on the loads. in the fbr nitrification efficiency also depends on ammonium load to the reactor and nitrite was accumulated when free ammonia concentration was ... | 2004 | 15137442 |
| nitrogen removal from sludge reject water by a two-stage oxygen-limited autotrophic nitrification denitrification process. | nitrogen removal from sludge reject water was obtained by oxygen-limited partial nitritation resulting in nitrite accumulation in a first stage, followed by autotrophic denitrification of nitrite with ammonium as electron donor (similar to anaerobic ammonium oxidation) in a second stage. two membrane-assisted bioreactors (mbrs) were used in series to operate with high sludge ages and subsequent high volumetric loading rates, achieving 1.45 kg n m(-3) day(-1) for the partial nitritation mbr and 1 ... | 2004 | 15137407 |
| ammonium and hydroxylamine uptake and accumulation in nitrosomonas. | starved cells of nitrosomonas europaea and further ammonia oxidizers were able to rapidly accumulate ammonium and hydroxylamine to an internal concentration of about 1 and 0.8 m, respectively. in kinetic studies, the uptake/accumulation rates for ammonium [3.1 mmol (g protein)(-1) min(-1)] and hydroxylamine [4.39 mmol (g protein)(-1) min(-1)] were determined. the uptake and accumulation process of ammonium and hydroxylamine was not coupled to ammonia or hydroxylamine oxidation and nitrite was no ... | 2004 | 15133102 |
| an analysis of nitrification during the aerobic digestion of secondary sludges. | investigations were undertaken to study the occurrence and progress of nitrification during aerobic digestion of activated sludge in a wide range of initial concentrations of total solids (1000 to 80 000 mg litre(-1), initial ph range of 4.5 to 10.4 and digestion temperature range of 5 degrees to 60 degrees c. batch aerobic digestion studies on activated sludge grown on wastewater (enriched with organic solids from human excretal material) indicate that almost complete elimination of the 'biodeg ... | 1989 | 15092439 |
| physiologic and proteomic evidence for a role of nitric oxide in biofilm formation by nitrosomonas europaea and other ammonia oxidizers. | no, a free radical gas, is the signal for nitrosomonas europaea cells to switch between different growth modes. at an no concentration of more than 30 ppm, biofilm formation by n. europaea was induced. no concentrations below 5 ppm led to a reversal of the biofilm formation, and the numbers of motile and planktonic (motile-planktonic) cells increased. in a proteomics approach, the proteins expressed by n. europaea were identified. comparison studies of the protein patterns of motile-planktonic a ... | 2004 | 15090520 |
| use of amob as a new molecular marker for ammonia-oxidizing bacteria. | specific molecular determination and classification of ammonia-oxidizing bacteria have relied on the use of conventional markers such as 16s rdna. however, this gene does not satisfactorily provide a wide vision of all phylogenetic lineages. despite the initial expectations, the use of functional genes as for example amoa has only been useful to corroborate the established taxonomy. ammonia-oxidizing bacteria constitute a physiological group that crosses over principal phylogenetic radiations. t ... | 2004 | 15003690 |
| start-up of autotrophic nitrogen removal reactors via sequential biocatalyst addition. | a procedure for start-up of oxygen-limited autotrophic nitrification-denitrification (oland) in a lab-scale rotating biological contactor (rbc) is presented. in this one-step process, nh4+ is directly converted to n2 without the need for an organic carbon source. the approach is based on a sequential addition of two types of easily available biocatalyst to the reactor during start-up: aerobic nitrifying and anaerobic, granular methanogenic sludge. the first is added as a source of aerobic ammoni ... | 2004 | 14998042 |
| genera-specific immunofluorescence labeling of ammonia oxidizers with polyclonal antibodies recognizing both subunits of the ammonia monooxygenase. | polyclonal antibodies that recognize the two subunits amoa and amob of the ammonia monooxygenase (amo) were applied to identify ammonia-oxidizing bacteria by immunofluorescence (if) labeling in pure, mixed, and enriched cultures. the antibodies against the amoa were produced using a synthetic peptide of the amoa of nitrosomonas eutropha, whereas the antibodies against the amob had been developed previously is against the whole b-subunit of the amo [pinck et al. (2001) appl environ microbiol 67:1 ... | 2004 | 14994172 |
| influence of soil moisture on linear alkylbenzene sulfonate-induced toxicity in ammonia-oxidizing bacteria. | moisture affects bioavailability and fate of pollutants in soil, but very little is known about moisture-induced effects on pollutant toxicity. we here report on a modifying effect of moisture on degradation of linear alkylbenzene sulfonates (lass) and on their toxicity towards ammonia-oxidizing bacteria (aob) in agricultural soil. in soil spiked with two las levels (250 or 1,000 mg/kg) and incubated at four different moisture levels (9-100% of water-holding capacity), degradation was strongly a ... | 2004 | 14982383 |
| a distinctive electrocatalytic response from the cytochrome c peroxidase of nitrosomonas europaea. | here the cytochrome c peroxidase (ccp) from nitrosomonas europaea is examined using the technique of catalytic protein film voltammetry. submonolayers of the bacterial diheme enzyme at a pyrolytic graphite edge electrode give catalytic, reductive signals in the presence of the substrate hydrogen peroxide. the resulting waveshapes indicate that ccp is bound non-covalently in a highly active configuration. the native enzyme has been shown to possess two heme groups of low and high potential (l and ... | 2004 | 14973133 |
| isolation of nitrifying organisms and oxygen supply. | 1952 | 14947885 | |
| inhibition of membrane-bound methane monooxygenase and ammonia monooxygenase by diphenyliodonium: implications for electron transfer. | diphenyliodonium (dpi) is known to irreversibly inactivate flavoproteins. we have found that dpi inhibits both membrane-bound methane monooxygenase (pmmo) from methylococcus capsulatus and ammonia monooxygenase (amo) of nitrosomonas europaea. the effect of dpi on nadh-dependent pmmo activity in vitro is ascribed to inactivation of ndh-2, a flavoprotein which we proposed catalyzes reduction of the quinone pool by nadh. dpi is a potent inhibitor of type 2 nadh:quinone oxidoreductase (ndh-2), with ... | 2004 | 14761987 |
| comparative analysis of genetic diversity and expression of amoa in wastewater treatment processes. | the genetic diversity and expression of amoa of autotrophic ammonia oxidizers in wastewater treatment processes were investigated by rt-pcr and denaturing gradient gel electrophoresis (dgge) in order to identify active components of ammonia-oxidizer populations in a such processes. ammonia oxidizers, evidenced by the presence of amoa mrna, were regarded as metabolically active. the dgge profiles derived from amoa mrna and from its gene, which were amplified by rt-pcr or pcr using samples collect ... | 2004 | 14758520 |
| analysis of size distribution and areal cell density of ammonia-oxidizing bacterial microcolonies in relation to substrate microprofiles in biofilms. | a fine-scale in situ spatial organization of ammonia-oxidizing bacteria (aob) in biofilms was investigated by combining molecular techniques (i.e., fluorescence in situ hybridization (fish) and 16s rdna-cloning analysis) and microelectrode measurements. important parameters of aob microcolonies such as size distribution and areal cell density of the microcolonies were determined and correlated with substrate microprofiles in the biofilms. in situ hybridization with a nested 16s rrna-targeted oli ... | 2004 | 14705015 |
| metabolism of inorganic n compounds by ammonia-oxidizing bacteria. | ammonia oxidizing bacteria extract energy for growth from the oxidation of ammonia to nitrite. ammonia monooxygenase, which initiates ammonia oxidation, remains enigmatic given the lack of purified preparations. genetic and biochemical studies support a model for the enzyme consisting of three subunits and metal centers of copper and iron. knowledge of hydroxylamine oxidoreductase, which oxidizes hydroxylamine formed by ammonia monooxygenase to nitrite, is informed by a crystal structure and det ... | 2003 | 14695127 |
| nitrification and nitrifying bacteria in the lower seine river and estuary (france). | the achères wastewater treatment plant, located just downstream of paris, discharges its effluents into the lower seine river. the effluents contain large numbers of heterotrophic bacteria, organic matter, and ammonium and are a source of nitrifying bacteria. as a result, degradation of organic matter by heterotrophic bacteria and subsequent oxygen depletion occur immediately downstream of the effluent outlet, whereas nitrifying bacteria apparently need to build up a significant biomass before a ... | 2003 | 14660353 |
| chemolithoorganotrophic growth of nitrosomonas europaea on fructose. | the nitrifying bacterium nitrosomonas europaea can obtain all its carbon for growth from co(2) and all its energy and reductant for growth from the oxidation of nh(3) and is considered an obligate chemolithoautotroph. previous studies have shown that n. europaea can utilize limited amounts of certain organic compounds, including amino acids, pyruvate, and acetate, although no organic compound has been reported to support the growth of n. europaea. the recently completed genomic sequence of n. eu ... | 2003 | 14617645 |
| characterization of nitrifying granules produced in an aerobic upflow fluidized bed reactor. | since nitrification is the rate-determining step in the biological nitrogen removal from wastewater, many research studies have been conducted on the immobilization of nitrifying bacteria. in this research, granulation of nitrifying bacteria in an aerobic upflow fluidized bed (aufb) reactor in a nitrification process for inorganic wastewater containing 500 g/m(3) of nh(4)(+)-n was investigated. it was observed that spherical, pseudocubic and elliptical granules with a diameter of 346 microm were ... | 2003 | 14604643 |
| the isotope array, a new tool that employs substrate-mediated labeling of rrna for determination of microbial community structure and function. | a new microarray method, the isotope array approach, for identifying microorganisms which consume a (14)c-labeled substrate within complex microbial communities was developed. experiments were performed with a small microarray consisting of oligonucleotide probes targeting the 16s rrna of ammonia-oxidizing bacteria (aob). total rna was extracted from a pure culture of nitrosomonas eutropha grown in the presence of [(14)c]bicarbonate. after fluorescence labeling of the rna and microarray hybridiz ... | 2003 | 14602652 |
| molecular analysis of microbial communities in nitrification and denitrification reactors treating high ammonia leachate. | molecular analysis of microbial populations in two bench-scale nitrification and denitrification reactors fed with high ammonia landfill leachate was conducted in this study by using dgge, cloning, and fish techniques in addition to classical efficiency control parameters. nitrification tank was operated with a computer-controlled alkalinity dosing system to supply the alkalinity intermittently as consumed on the basis of on-line ph monitoring. by keeping the ph at 7.0 with this system, 99% nitr ... | 2003 | 14524656 |
| a comparitive study of ammonia-oxidizing bacteria in lab-scale industrial wastewater treatment reactors. | the diversity and community structure of the beta-proteobacterial ammonia oxidising bacteria (aob) in a range of different lab-scale industrial wastewater treatment reactors were compared. three of the reactors treat waste from mixed domestic and industrial sources whereas the other reactor treats waste solely of industrial origin. pcr with aob selective primers was combined with denaturing gradient ge electrophoresis to allow comparative analysis of the dominant aob populations and the phylogen ... | 2003 | 14518850 |
| dynamic response of nitrifying activated sludge batch culture to increased chloride concentration. | dynamic response of nitrifying activated sludge batch cultures to increased chloride concentration was studied in this paper, which focused upon the changes in the specific nitrification rate (snr) and nitrifier population when the chloride level was gradually or stepwise increased to 30,000 mg cl l-1. the dominant species of ammonia-oxidizers and nitriteoxidizers in the population were examined by fluorescent in situ hybridization technique with 16s rrna-targeted oligonucleotide probes. it was ... | 2003 | 14509699 |
| growth rates of nitrifying chemoautotrophs in soil. | 1962 | 14476222 | |
| [morphology of nitrosomonas europaea]. | 1960 | 14439829 | |
| [relation of the process of oxidation of ammonium ion by nitrite bacteria to certain factors of the medium. i. effect of concentrations of ammonium sulfate]. | 1959 | 14418616 | |
| [relation of nh4 ion oxidation processes by nitrite bacteria to certain environmental factors. ii. effect of temperature]. | 1959 | 14418614 | |
| [verification of purity of nitrosomonas cultures]. | 1955 | 14383368 | |
| [myxobacillus accepted as nitrifying bacteria]. | 1955 | 14383367 | |
| [comparative studies on the effect of visible light on nitrosomonas europaea and nitrobacter winogradskyi]. | 1965 | 14347921 | |
| studies on the oxidation of ammonia by nitrosomonas. | 1. free-energy calculations for ph7 showed that the oxidation of ammonia to hydroxylamine is endergonic and that the oxidations of hydroxylamine to nitrite and hydrazine to nitrogen are exergonic. it is suggested that the oxidation of ammonia requires the expenditure of energy. 2. the anaerobic dehydrogenation of hydrazine to nitrogen by extracts of the autotrophic nitrifying micro-organism, nitrosomonas, in the presence of methylene blue as electron acceptor, was less rapid than the anaerobic d ... | 1965 | 14342504 |
| estimation of the nitric oxide formed from hydroxylamine by nitrosomonas. | 1. nitric oxide that was produced by reducing nitrite with an excess of acidified potassium iodide under nitrogen in warburg respirometer flasks was rapidly absorbed by a solution of permanganate in sodium hydroxide held in the side arm. a small amount of nitrous oxide (or nitrogen) that was also produced was not absorbed. 2. by using a quantitative method for the recovery of nitrite from samples of the alkaline permanganate, it was found that the sum of the nitrite n formed and the residual nit ... | 1965 | 14342235 |
| continuous flow method in soil microbiology. v. nitrification. | 1965 | 14332135 | |
| [studies on the influence of temperature on nitrification in the elbe estuary]. | 1964 | 14320216 | |
| the effect of 2-chloro, 6-(trichloromethyl) pyridine on the chemoautotrophic metabolism of nitrifying bacteria. i. ammonia and hydroxylamine oxidation by nitrosomonas. | 1965 | 14315631 | |
| structure of nitrosocystis oceanus and comparison with nitrosomonas and nitrobacter. | murray, r. g. e. (university of western ontario, london, ont., canada), and s. w. watson. structure of nitrosocystis oceanus and comparison with nitrosomonas and nitrobacter. j. bacteriol. 89:1594-1609. 1965.-nitrosocystis oceanus has distinctive features: the cell wall (overall thickness, 250 a) has an inner triplet structure and a dense enveloping layer; between these lie the "cell-wall organelles" (two or more per cell; plaques about 0.5 mu in diameter and 0.1 mu thick) of unknown function an ... | 1965 | 14293026 |
| studies on the formation of nitrogenous gas from hydroxylamine by nitrosomonas. | 1965 | 14292843 | |
| determination of kinetic constants for nitrifying bacteria in mixed culture, with the aid of an electronic computer. | 1965 | 14287204 | |
| hydroxylamine formation by nitrosomonas europaea. | 1964 | 14272491 | |
| [the behavior of nitrifying bacteria towards dissolved oxygen]. | 1964 | 14245150 | |
| biochemistry of the cell wall of nitrosomonas europaea. | 1964 | 14200691 | |
| the synthesis of atp and the incorporation of 32p by cell-free preparations from nitrosomonas europaea. | 1964 | 14192897 | |
| occurrence of cytochrome oxidase and peroxidase in nitrosomonas europaea. | 1964 | 14165498 | |
| the incorporation of labelled co2 into cells and extracts of nitrosomonas europaea. | 1964 | 14123573 | |
| some organic transformation effected by cell-free preparations of nitrosomonas. | 1963 | 14099563 | |
| some properties of a hydroxylamine oxidase from nitrosomonas europaea. | 1963 | 14090439 | |
| adenosine triphosphate-dependent reduction of nicotinamide adenine dinucleotide by ferro-cytochrome c in chemoautotrophic bacteria. | 1963 | 14087009 | |
| [bacterial associations and biological alterations of limestone monuments]. | 1963 | 14048726 | |
| phosphorylation by extracts of nitrosomonas europaea. | burge, w. d. (university of california, berkeley), e. malavolta, and c. c. delwiche. phosphorylation by extracts of nitrosomonas europaea. j. bacteriol. 85:106-110. 1963.-cellfree preparations of nitrosomonas europaea are capable of oxidizing hydroxylamine, but not ammonium ion, to nitrite. the quantity of nitrite formed by our preparations was, at most, equivalent to only 70% of the hydroxylamine added. although the preparations had a strong phosphatase activity, resulting in a net loss of orga ... | 1963 | 14016952 |
| autotrophic enzyme systems. i. electron transport systems concerned with hydroxylamine oxidation in nitrosomonas. | 1963 | 14011836 | |
| use of electron paramagnetic resonance spectroscopy in investigations of functional metal components in micro-organisms. | 1962 | 13938326 | |
| [the physiological role of cytochrome in nitrifying bacteria]. | 1962 | 13931334 | |
| [adaptation of nitrosomonas ectotypes to concentrations of organic substances in culture media]. | 1962 | 13923650 | |
| [the effect of light on nitrosomonas europaea win]. | 1962 | 13909041 | |
| nitrohydroxyl-amine: the unknown intermediate in nitrification? | 1962 | 13860225 | |
| enzymatic activity of nitrosomonas extracts. | 1959 | 13820316 | |
| [on the ecology of nitrosomonas]. | 1961 | 13779066 | |
| [nitrifying activity of pure and accumulated nitrosomonas cultures isolated from various natural substrates]. | 1961 | 13779065 | |
| [isolation of pure cultures of nitrosomonas from various natural substrates and their characteristics]. | 1960 | 13779064 | |
| [adaptation of nitrosomonas to conditions of various natural substrates]. | 1961 | 13779063 | |
| [the pigmented form of nitrosomonas europaea]. | 1960 | 13763903 | |
| [is nitrosomonas capable of assimilating organic substances from the air?]. | 1960 | 13726365 | |
| morphology of nitrosomonas europaea and classification of the nitrifying bacteria. | 1961 | 13696807 | |
| [nitrogen metabolism in nitrosomonas europaea]. | 1958 | 13622242 | |
| isolation of nitrosomonas in pure culture. | 1958 | 13598713 | |
| [the behavior of nitrosomonas europaea after dessication]. | 1958 | 13589713 | |
| influence of corn steep liquor on the oxidation of ammonia to nitrate by nitrosomonas europaea. | 1958 | 13575767 | |
| growth and autotrophic metabolism of nitrosomonas europaea. | 1958 | 13563419 | |
| [relation of nitrosomonas to radioactive saccharose and carbonate]. | 1957 | 13483120 | |
| [accumulation of organic substances by nitrosomonas europaea cultures grown on winogradsky's medium]. | 1957 | 13477106 | |
| preservation of nitrosomonas. | 1957 | 13440942 | |
| preservation of nitrosomonas. | 1957 | 13418790 | |
| [possibility of heterotrophic metabolism in nitrifying bacteria]. | 1956 | 13407450 | |
| [acellular nitrification. v. oxidation of hydroxylamine in acellular autolysates of nitrosomnas]. | 1956 | 13369172 | |
| estimation of numbers of nitrosomonas in soil and culture. | 1956 | 13321980 | |
| [relation of nitrosomonas to organic substances]. | 1956 | 13321539 | |
| [acellular nitrification. iv. heat inactivation of ferments of nitrosomonas europea oxidizing ammonia]. | 1956 | 13321524 | |
| [acellular nitrifikation. iii. dynamics of concentration of nitrites]. | 1955 | 13296884 | |
| [acellular nitrification. ii. oxidation of ammonia with autolysates of nitrosomonas]. | 1954 | 13223516 | |
| [acellular nitrification. i. growth of cultures of nitrosomas and formation of cell autolysates]. | 1954 | 13203080 | |
| [isolation of pure cultures of nitrosomonas europae]. | 1954 | 13173366 | |
| [chemistry of nitrification]. | 1954 | 13151064 | |
| [obtaining pure cultures of nitrosomonas]. | 1953 | 13144375 | |
| 16s rrna and amoa-based phylogeny of 12 novel betaproteobacterial ammonia-oxidizing isolates: extension of the dataset and proposal of a new lineage within the nitrosomonads. | the phylogenetic relationship of 12 ammonia-oxidizing isolates (eight nitrosospiras and four nitrosomonads), for which no gene sequence information was available previously, was investigated based on their genes encoding 16s rrna and the active site subunit of ammonia monooxygenase (amoa). almost full-length 16s rrna gene sequences were determined for the 12 isolates. in addition, 16s rrna gene sequences of 15 ammonia-oxidizing bacteria (aob) published previously were completed to allow for a mo ... | 2003 | 13130037 |
| laboratory studies on the kinetics of the growth of nitrosomonas with relation to the nitrification phase of the b.o.d. test. | 1954 | 13125339 | |
| [oxidation of ammonia and synthesis of protein in pure cultures of nitrosomonas europea]. | 1953 | 13116891 | |
| [feeding of nitrosomonas europea on organic substances]. | 1953 | 13116876 | |
| [development of heterotrophes in inorganic medium and role of ammonium]. | 1953 | 13095323 | |
| the biochemistry of the nitrifying organisms. iv. the respiration and intermediary metabolism of nitrosomonas. | 1953 | 13058954 | |
| the biochemistry of the nitrifying organisms. iii. composition of nitrosomonas. | 1953 | 13058873 | |
| composition and cytology of nitrosomonas. | 1953 | 13037653 | |
| iron and the nitrifying bacteria. | 1953 | 13035032 | |
| the intermediary metabolism of nitrosomonas. | 1952 | 13018312 | |
| the respiration of nitrosomonas. | 1952 | 13018311 | |
| the biochemistry of the nitrifying organisms. ii. the free-energy efficiency of nitrosomonas. | 1952 | 13018178 | |
| the biochemistry of the nitrifying organisms. i. the ammonia oxidizing systems of nitrosomonas. | 1952 | 13018177 | |
| [pure cultures of nitrosomonas]. | 1952 | 12998527 | |
| wide distribution of a novel pmoa-like gene copy among type ii methanotrophs, and its expression in methylocystis strain sc2. | experiments were conducted to determine if a novel pmoa-like gene (pmoa2) recently discovered in the methane-oxidizing bacterium methylocystis strain sc2 (p. f. dunfield, m. tchawa yimga, s. d. dedysh, u. berger, w. liesack, and j. heyer, fems microbiol. ecol. 41:17-26, 2002) is present in other methane-oxidizing bacteria (mob), and if it is expressed. a newly developed primer combination (pmoa206f-pmoa703b) allowed a differential detection of pmoa1 and pmoa2. by using this primer combination, w ... | 2003 | 12957949 |
| characterization of microbial communities and composition in constructed dairy wetland wastewater effluent. | constructed wetlands have been recognized as a removal treatment option for high concentrations of contaminants in agricultural waste before land application. the goal of this study was to characterize microbial composition in two constructed wetlands designed to remove contaminants from dairy washwater. water samples were collected weekly for 11 months from two wetlands to determine the efficiency of the treatment system in removal of chemical contaminants and total and fecal coliforms. the red ... | 2003 | 12957887 |
| molecular biological methods (dgge) as a tool to investigate nitrification inhibition in wastewater treatment. | incomplete nitrification at an activated sludge plant for biological pre-treatment of rendering plant effluents led to a detailed investigation on the origin and solution of this problem. preliminary studies revealed that an inhibition of ammonia oxidising microorganisms (aom) by process waters of the rendering plant was responsible for the situation. we were able to show a correlation between the existence of specific aom and nitrification capacity expressed as oxygen uptake rate for maximal ni ... | 2003 | 12906286 |
| linking nitrifying biofilm structure and function through fluorescent in situ hybridization and evaluation of nitrification capacity. | fluorescent in situ hybridization (fish) was used to quantify the ammonia-oxidizing populations within intact biofilm samples collected from a full-scale nitrifying trickling filter (ntf). ammonia, nitrite, and nitrate concentrations were measured for aqueous samples taken in conjunction with biofilm samples at multiple filter depths. the ammonia removal capability of collected biofilms was evaluated by transferring the biofilms to laboratory batch reactors. nitrate plus nitrite generation rates ... | 2003 | 12837026 |