Publications

structural and kinetic insights into the mechanism of 5-hydroxyisourate hydrolase from klebsiella pneumoniae.	the stereospecific oxidative degradation of uric acid to (s)-allantoin has recently been demonstrated to proceed via two unstable intermediates and requires three separate enzymatic reactions. the second step of this reaction, the conversion of 5-hydroxyisourate (hiu) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline, is catalyzed by hiu hydrolase (hiuh). the high-resolution crystal structure of hiuh from the opportunistic pathogen klebsiella pneumoniae (kphiuh) has been determined. kphiuh is a h ...	2011	21795808
a call to arms: the imperative for antimicrobial stewardship.	antimicrobial resistance is a major public health crisis. the prevalence of drug-resistant organisms, such as the emerging nap1 strain of clostridium difficile, now highly resistant to fluoroquinolones, acinetobacter species, klebsiella pneumoniae carbapenemase-producing organisms, and methicillin-resistant staphylococcus aureus, is increasing nationwide. the sources of antimicrobial resistance are manifold, but there is a well-documented causal relationship between antimicrobial use and misuse ...	2011	21795727
synthesis and studies of novel 2-(4-cyano-3-trifluoromethylphenyl amino)-4-(quinoline-4-yloxy)-6-(piperazinyl/piperidinyl)-s-triazines as potential antimicrobial, antimycobacterial and anticancer agents.	a series of novel s-triazine analogs were synthesized and characterized by ir, (1)h nmr, (13)c nmr, (19)f nmr spectroscopy and elemental analysis. preliminary screening of target compounds against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneria), four fungi (aspergillus niger, aspergillus fumigatus, aspergillus clavatus, candida albicans) and mycobacterium tuberculosis ...	2011	21794959
contribution of mucoviscosity associated gene a (maga) to virulence in experimental klebsiella pneumoniae endophthalmitis.	purpose: endogenous endophthalmitis secondary to klebsiella pneumoniae liver abscess is a blinding infection that is being reported more frequently in the literature. the k1 capsule and maga contribute to virulence of systemic infection in mice, however, little is known about the role of maga in secondary ocular infections. methods: to assess the role of k. pneumoniae capsule in endophthalmitis, we induced experimental endophthalmitis by direct inoculation of 100 cfu of wild type, maga-deficient ...	2011	21791595
heat shock protein 60 from klebsiella pneumoniae protects mononuclear cells from apoptotic cell death induced by dexamethasone.	aims: bacterial heat shock proteins can have anti-apoptotic effects on human cells. we investigated whether enterobacterial hsp60 can protect peripheral blood mononuclear cells (pbmc) from dxm-induced apoptosis and if this effect requires cytoskeleton participation. main methods: anti-apoptotic effect from enterobacterial hsp60 was analyzed by adding these proteins to peripheral mononuclear cells cultures before dxm induction. percentage of apoptotic cells was determined by subg0 peak and tunel ...	2011	21791241
antibacterial efficacy of lytic bacteriophages against antibiotic-resistant klebsiella species.	bacterial resistance to antibiotics is a leading and highly prevalent problem in the treatment of infectious diseases. bacteriophages (phages) appear to be effective and safe alternatives for the treatment of resistant infections because of their specificity for bacterial species and lack of infectivity in eukaryotic cells. the present study aimed to isolate bacteriophages against klebsiella spp. and evaluate their efficacy against antibiotic-resistant species. seventy-two antibiotic-resistant k ...	2011	21789469
safety of pediatric percutaneous liver biopsy performed by interventional radiologists.	: national data suggest that pediatric percutaneous liver biopsy is increasingly being performed by interventional radiologists rather than pediatric gastroenterologists. the objective of the present report is to describe the safety and effectiveness of percutaneous liver biopsy performed by interventional radiologists in a large cohort of children and to compare the results with the existing literature on biopsies performed by pediatric gastroenterologists.	2011	21788763
further increases in carbapenem-, amikacin-, and fluoroquinolone-resistant isolates of acinetobacter spp. and p. aeruginosa in korea: konsar study 2009.	purpose: the increasing prevalence of antimicrobial resistant bacteria has become a serious worldwide problem. the aim of this study was to analyze antimicrobial resistance data generated in 2009 by hospitals and commercial laboratories participating in the korean nationwide surveillance of antimicrobial resistance program. materials and methods: susceptibility data were collected from 24 hospitals and two commercial laboratories. in the analysis, resistance did not include intermediate suscepti ...	2011	21786445
in vitro activity of 5-(2,4-dimethylbenzyl) pyrrolidin-2-one extracted from marine streptomyces vitsvk5 spp. against fungal and bacterial human pathogens.	background: pharmacological screening and usage of natural products for the treatment of human diseases has had a long history from traditional medicine to modern drugs. the majority of modern drugs are reported to be mostly from natural products. objective: the aim of the present study was to evaluate the inhibitory activity of 5-(2,4-dimethylbenzyl) pyrrolidin-2-one (dmbpo) extracted from marine streptomyces vitsvk5 spp. isolated from sediment samples collected at marakkanam coast of bay of be ...	2011	21782963
design and synthesis of spiro[indole-thiazolidine]spiro[indole-pyrans] as antimicrobial agents.	a series of novel spiro[indole-thiazolidine]spiro[indole-pyran] derivatives were synthesized from n-(bromoalkyl)indol-2,3-diones via monospiro-bisindole intermediates; the two indole nuclei being connected via n-(ch(2))(n)-n linker. synthesized compounds were evaluated for their antimicrobial activities in vitro against three gram-positive bacteria (staphylococcus aureus, bacillus subtilis, and staphylococcus epidermis), four gram-negative bacteria (escherichia coli, pseudomonas aeruginosa, salm ...	2011	21782421
rising incidence of acute prostatitis following prostate biopsy: fluoroquinolone resistance and exposure is a significant risk factor.	objectives: to evaluate the frequency and potential risk factors for infection-related complications after transrectal prostate biopsy and to propose adjustments in current antimicrobial prophylaxis recommendations. methods: during 2008-2010, 107 patients underwent transrectal ultrasound-guided biopsies of the prostate at our institution. charts were reviewed for infection-related complications within 30 days of the procedure. potential risk factors were evaluated, including age, diabetes mellit ...	2011	21782225
late-onset klebsiella oxytoca flap-margin-related corneal ulcer following laser in situ keratomileusis.	a 45-year-old woman developed a spontaneous flap-margin-related corneal ulcer 11 years after the original laser in situ keratomileusis (lasik) procedure. two enhancements had been performed; the most recent was 3 years prior to presentation. cultures were positive for a heavy growth of klebsiella oxytoca. the ulcer responded clinically to topical treatment with fortified cefazolin. eighteen days after presentation, the infiltrate had resolved and the cornea had fully epithelialized. to our knowl ...	2011	21782101
[clinical and economic expedience of ertapenem therapy of complicated urinary tract infection].	clinical and economic investigation of various antibiotics use in the treatment of complicated urinary tract infection (cuti) was performed under the russian economic environment. the drugs of comparison were ertapenem, ceftriaxone and levofloxacin. direct costs and their structure were shown, and the cost efficiency was calculated. alternative analysis and one-side susceptibility analysis were performed. in complicated urinary tract infections when the major pathogens were escherichia coli, kle ...	2011	21780670
[antimicrobial activity of bacteriocin s760 produced by enterococcus faecium strain lwp760].	antimicrobial activity of bacteriocin s760 (enterocin) produced by enterococcusfaecium strain lwp760 was studied. bacteriocin s760 is a cationic, hydrophobic, and heat stable peptide with the molecular weight of 5.5 kda and pl of 9.8. enterocin s760 is shown to inhibit in vitro the growth both of sensitive and resistant to antibacterials gramnegative and grampositive bacteria of 25 species. mics of the bacteriocin s760 vary between 0.05-1.6 mg/l for escherichia coli 0157:h117, salmonella typhimu ...	2011	21780664
impact of hfq on global gene expression and virulence in klebsiella pneumoniae.	klebsiella pneumoniae is responsible for a wide range of clinical symptoms. how this bacterium adapts itself to ever-changing host milieu is still a mystery. recently, small non-coding rnas (srnas) have received considerable attention for their functions in fine-tuning gene expression at a post-transcriptional level to promote bacterial adaptation. here we demonstrate that hfq, an rna-binding protein, which facilitates interactions between srnas and their mrna targets, is critical for k. pneumon ...	2011	21779404
[a case of emphysematous gastritis in a patient with end-stage renal disease].	emphysematous gastritis is a rare infection of the stomach wall with high mortality rate. it is caused by gas forming organisms and may arise by local spread through the mucosa or hematogenous dissemination from distant focus. clinical manifestation includes acute abdomen with systemic toxicity, and diagnosis is based on radiologic demonstration of gas within the gastric wall. treatment should be aimed to cover gram-negative organisms and anaerobes using wide-spectrum intravenous antibiotics, an ...	2011	21778802
high-mobility group protein n2 (hmgn2) inhibited the internalization of klebsiella pneumoniae into cultured bladder epithelial cells.	since bacterial invasion into host cells is an important step in the infection process, using the agents to interfere with bacterial internalization is an attractive approach to block the infection process. in this work, we describe a new, previously unrecognized role of the human cationic host defense peptide hmgn2 during klebsiella pneumoniae infections. our results revealed that the internalization of k. pneumoniae strain 03183 into cultured bladder epithelial cells (t24) was significantly re ...	2011	21778192
clinical predictors of pseudomonas aeruginosa bacteremia among gram-negative bacterial infections in non-neutropenic patients with solid tumor.	objectives: this study was performed to identify risk factors in pseudomonas aeruginosa bacteremia among gram-negative bacterial infections in non-neutropenic patients with solid tumor. methods: a case-control study was performed to identify clinical predictors for p. aeruginosa bacteremia among non-neutropenic patients with gram-negative bacteremia. each case of p. aeruginosa bacteremia was matched to one or two controls with escherichia coli, klebsiella, enterobacter or citrobacter species in ...	2011	21777616
cryptogenic pyogenic liver abscess as the herald of colon cancer.	background and aim: colonic mucosal defects might be a route for bacterial invasion into the portal system with subsequent hematogenous spread to the liver. we retrospectively investigated the results of colonoscopy and clinical characteristics of patients with pyogenic liver abscess of colonic origin. methods: a total of 230 consecutive patients with pyogenic liver abscess were reviewed between 2003 and 2010. the 230 patients were categorized into three groups [pancreato-biliary (n = 135), cryp ...	2011	21777280
longitudinal survey of carbapenem resistance and resistance mechanisms in enterobacteriaceae and non-fermenters from the usa in 2007-09.	background antibiotic resistance is problematic in enterobacteriaceae, pseudomonas aeruginosa and acinetobacter baumannii, and is often associated with serious infections. carbapenems are often one of the few remaining therapeutic options, so it is important to monitor carbapenem activity against these pathogens and to identify resistance mechanisms. methods carbapenem susceptibilities were determined for 14?359 enterobacteriaceae, 3614 p. aeruginosa and 994 a. baumannii from the usa (2007-09). ...	2011	21775338
crystal structure of new delhi metallo-ß-lactamase reveals molecular basis for antibiotic resistance.	ß-lactams are the most commonly prescribed class of antibiotics and have had an enormous impact on human health. thus, it is disquieting that an enzyme called new delhi metallo-ß-lactamase-1 (ndm-1) can confer enterobacteriaceae with nearly complete resistance to all ß-lactam antibiotics including the carbapenams. we have determined the crystal structure of klebsiella pneumoniae apo-ndm-1 to 2.1-å resolution. from the structure, we see that ndm-1 has an expansive active site with a unique electr ...	2011	21774017
methanolic extracts of aloe secundiflora engl. inhibits in vitro growth of tuberculosis and diarrhea-causing bacteria.	the emergence of resistance to antimicrobials by pathogens has reached crisis levels, calling for identification of alternative means to combat diseases.	2011	21772752
preliminary studies for a new antibiotic from the marine mollusk melo melo (lightfoot, 1786).	to search for new bioactive compounds from marine mollusks melo melo (m. melo).	2011	21771476
bla(ctx-m), bla(tem), and bla(shv) in enterobacteriaceae from north-indian tertiary hospital: high occurrence of combination genes.	to delineate the frequency of occurrence of bla(ctx-m), bla(tem), and bla(shv) in enterobacteriaceae from north-indian tertiary hospital.	2011	21771430
isolation and identification of bioactive antibacterial components in leaf extracts of vangueria spinosa (rubiaceae).	the column chromatographic fraction of ethyl acetate (ea1, ea2, ea3, ea4 and ea5) leaf extracts of vangueria spinosa (v. spinosa) were screened for antibacterial activity and phytochemical analysis.	2011	21771412
transmission of extended-spectrum ß-lactamase klebsiella oxytoca via the breathing circuit of a transport ventilator: root cause analysis and infection control recommendations.		2011	21768772
production of kpc-2 carbapenemase by an escherichia coli clinical isolate belonging to the international st131 clone.	the rapid international dissemination of klebsiella pneumoniae carbapenemase (kpc) producing organisms is of major concern.…	2011	21768521
emergence of oxa-48-producing escherichia coli clone st38 in france.	the bla(oxa-48) gene encodes a carbapenem-hydrolyzing class d ß-lactamase that was first identified in klebsiella pneumoniae from turkey in 2003 and then extensively in that country (1, 11).…	2011	21768512
characterization of oxa-181, a carbapenem-hydrolyzing class d {beta}-lactamase from klebsiella pneumoniae.	klebsiella pneumoniae kp3 was isolated from a patient, transferred from india to the sultanate of oman. k. pneumoniae kp3 was resistant to all ß-lactams including carbapenems and expressed the carbapenem-hydrolyzing ß-lactamase oxa-181, which differs from oxa-48 by four amino acid substitutions. compared to oxa-48, oxa-181 possessed a very similar hydrolytic profile. the bla(oxa-181) gene was located on a 7.6-kb cole-type plasmid and was linked to insertion sequence isecp1. the isecp1-mediated o ...	2011	21768505
laboratory evaluation of the chromagar kpc medium for identification of carbapenem-nonsusceptible enterobacteriaceae.	chromagar kpc was evaluated with 53 carbapenem-nonsusceptible strains. kpc-producing klebsiella pneumoniae and enterobacter cloacae grew efficiently and yielded metallic blue colonies (100% sensitivity). poor or no growth or atypical colony color were evident with kpc-producing escherichia coli (71.4% sensitivity) and ertapenem-resistant kpc-nonproducing k. pneumoniae (72.7% sensitivity). chromagar kpc appears applicable for processing carbapenem-resistant enterobacteriaceae surveillance specime ...	2011	21767716
detection of synergy using the combination of polymyxin b with either meropenem or rifampin against carbapenemase-producing klebsiella pneumoniae.	polymyxin b (pb) plus meropenem (mer) or rifampin (rif) was tested by etest® method and time-kill assay (tka) against 14 genetically unique clinical klebsiella pneumoniae carbapenemase-producing k. pneumoniae. pb + mer: etest, 43% synergy; tka, 64% synergy. concordance between methods was 79%. for pb + rif: etest, 21% synergy; tka, 100% synergy. concordance between methods was 21%.	2011	21767715
accelerated production of 1,3-propanediol from glycerol by klebsiella pneumoniae using the method of forced ph fluctuations.	1,3-propanediol (1,3-pd) is a bivalent alcohol, used in a number of chemical syntheses. it could be produced from glycerol in course of microbial fermentation by klebsiella pneumoniae along with more than five minor liquid products. with the purpose to enhance 1,3-pd production and to eliminate by-products formation, principally new ph control on the process was applied. the method, named "forced ph fluctuations" was realized by consecutive raisings of ph with definite ?ph amplitude (ranging fro ...	2011	21766234
functions of some capsular polysaccharide biosynthetic genes in klebsiella pneumoniae ntuh k-2044.	the growing number of klebsiella pneumoniae infections, commonly acquired in hospitals, has drawn great concern. it has been shown that the k1 and k2 capsular serotypes are the most detrimental strains, particularly to those with diabetes. the k1 cps (capsular polysaccharide) locus in the ntuh-2044 strain of the pyogenic liver abscess (pla) k. pneumoniae has been identified recently, but little is known about the functions of the genes therein. here we report characterization of a group of cps g ...	2011	21765903
long-term clinical and economic benefits associated with the management of a nosocomial outbreak resulting from extended-spectrum beta-lactamase-producing klebsiella pneumoniae.	objective:: in 2005, there was an epidemic of infections resulting from extended-spectrum ß-lactamase-producing klebsiella pneumoniae in the intensive care department. the aim of this study was to evaluate the potential long-term clinical and economic benefits resulting from the management of this epidemic and the resulting changes in practices. design:: two periods were defined: the period leading up to and including the epidemic (2003-2005; period i) and the postepidemic period (2006-2008; per ...	2011	21765349
fate of nitrogen-fixing bacteria in crude oil contaminated wetland ultisol.	the effect of crude oil on the growth of legumes (calopogonium muconoides and centrosema pubescens) and fate of nitrogen-fixing bacteria in wetland ultisol was investigated using standard cultural techniques. the results revealed observable effects of oil on soil physico-chemistry, plant growth and nodulation as well as on densities of heterotrophic, hydrocarbonoclastic and nitrogen fixing bacteria. the effects however varied with different levels (0.5%, 1%, 5%, 10%, 15% and 20%) of pollution. a ...	2011	21755289
iliopsoas abscess and spondylodiscitis by staphylococcus aureus: successful clinical treatment.	although pyogenic abscess of the iliopsoas muscle is considered an uncommon condition, its incidence is on the increase. this abscess may be primary if it occurs without a causal factor; more frequently, it is secondary to gastrointestinal or genitourinary affections, or trauma. the main aetiological agents differ among primary (staphylococcus aureus) and secondary (escherichia coli, klebsiella spp, streptococcus spp, bacteroides spp) abscess. we report the case of a 64-year-old man with iliopso ...	2011	21753252
susceptibility of esbl-producing enterobacteriaceae with the new clsi breakpoints.	in 2010 clsi lowered the susceptibility breakpoints of some cephalosporins and aztreonam for enterobacteriaceae and eliminated the need to perform esbl screening and confirmatory tests. the aim of this study was to determine how many esbl-producing strains will test susceptible for three common species of enterobacteriaceae using the new breakpoints. as determined with the clsi screening and confirmatory tests, 382 consecutive esbl-producing strains were collected at our hospital between 2007 an ...	2011	21752977
antioxidant, electrochemical, thermal, antimicrobial and alkane oxidation properties of tridentate schiff base ligands and their metal complexes.	in this study, two schiff base ligands (hl(1) and hl(2)) and their cu(ii), co(ii), ni(ii), pd(ii) and ru(iii) metal complexes were synthesized and characterized by the analytical and spectroscopic methods. alkane oxidation activities of the metal complexes were studied on cyclohexane as substrate. the ligands and their metal complexes were evaluated for their antimicrobial activity against corynebacterium xerosis, bacillus brevis, bacillus megaterium, bacillus cereus, mycobacterium smegmatis, st ...	2011	21752697
essential oils from moroccan plants as potential chemosensitisers restoring antibiotic activity in resistant gram-negative bacteria.	bacterial drug resistance is a worrying public health problem. antibiotic efflux is a major non-specific resistance mechanism used by bacteria, and efflux pumps are involved in the low-level susceptibility of various important gram-negative pathogens. use of molecules that can block bacterial pumps is an attractive strategy, but several studies report only partial efficacy owing to limits of these molecules (stability, selectivity, bioavailability, toxicity, etc.). the objective of this study wa ...	2011	21752605
impedimetric biosensor based on self-assembled hybrid cystein-gold nanoparticles and cramoll lectin for bacterial lipopolysaccharide recognition.	we report the development of a new selective and specific electrochemical biosensor for bacterial lipolysaccharide (lps). an electrode interface was constructed using a l-cysteine-gold nanoparticle (aunpcys) composite to be immobilized by electrostatic interaction in the network of a poly(vinyl chloride-vinyl acetate maleic acid) (pvm) layer on a gold bare electrode. the impedimetric biosensor is fabricated by self-assembled cramoll lectin on the pvm-aunpcys-modified gold electrode through elect ...	2011	21752390
incidence of esbl producers amongst gram-negative bacilli isolated from intra-abdominal infections across india (based on smart study, 2007 data).	this study was conducted in 9 centers spread over india from january 1 to december 31, 2007 to monitor in vitro susceptibility of gram-negative bacilli to group i carbapenem, ertapenem and other antimicrobials in intra-abdominal infections and to identify early changes in susceptibility pattern of community or hospital acquired organisms, with a focus on esbl producers.	2011	21751604
klebsiella pneumoniae bacteremia and capsular serotypes, taiwan.	capsular serotypes of 225 klebsiella pneumoniae isolates in taiwan were identified by using pcr. patients infected with k1 serotypes (41 isolates) had increased community-onset bacteremia, more nonfatal diseases and liver abscesses, lower pittsburgh bacteremia scores and mortality rates, and fewer urinary tract infections than patients infected with non-k1/k2 serotypes (147 isolates).	2011	21749784
metallo-ß-lactamase-producing enterobacteriaceae isolates at a taiwanese hospital: lack of distinctive phenotypes for screening.	liao i-c, chen h-m, wu j-j, tsai p-f, wang l-r, yan j-j. metallo-ß-lactamase-producing enterobacteriaceae isolates at a taiwanese hospital: lack of distinctive phenotypes for screening. apmis 2011; 119: 543-50. we investigated the prevalence of metallo-ß-lactamases (mbls) among 1,827 enterobacteriaceae isolates collected in 2006 and evaluated the vitek 2 microbiology system, modified hodge test, and 2 combined disk tests as the screening tools for mbls by using these isolates and 77 previously c ...	2011	21749455
extended-spectrum +¦-lactamase (esbl) in omani children: study of prevalence, risk factors and clinical outcomes at sultan qaboos university hospital, sultanate of oman.	antimicrobial resistance is a growing problem worldwide, which imposes difficulties in the selection of appropriate empirical antimicrobial therapy. this study evaluated extended-spectrum +¦-lactamase (esbl) isolates in 2005 in the department of child health at sultan qaboos university hospital (squh), oman.	2008	21748055
first outbreak of a plasmid-mediated carbapenem hydrolyzing oxa-48 {beta}-lactamase in klebsiella pneumoniae in spain.	twenty klebsiella pneumoniae isolates producing oxa-48 were collected from april-2009 to september-2010. strains were clonally related and co-produced a ctx-m-15 ß-lactamase. a conjugative plasmid of circa 70kb carrying bla(oxa-48) was identified. eleven isolates showed low-level resistance to carbapenems, whereas nine showed high-level resistance. decreased expression of ompk36 participated in high-level resistance to carbapenems. this strain belonged to sequence type 101 (st101). this is the f ...	2011	21746954
comparative genomics of klebsiella pneumoniae strains with different antibiotic resistance profiles.	there is a global emergence of multidrug resistant (mdr) strains of klebsiella pneumoniae, a gram-negative enteric bacterium that cause nosocomial and urinary tract infections. while the epidemiology of k. pneumoniae strains and occurrences of specific antibiotic resistance genes, such as plasmid-borne extended spectrum ß-lactamases (esbls), have been extensively studied, only four complete genomes of k. pneumoniae are available. to better understand the mdr factors in k. pneumoniae, we determin ...	2011	21746949
detection of a vim-27-producing klebsiella pneumoniae in a patient following surgical tourism in greece.	very recently papagiannitsis described vim-27, a new metallo-beta-lactamase (mbl) single point variant of vim-1, identified in three klebsiella pneumoniae isolates of sequence type (st) 147 recovered at three different greeks hospitals (6).…	2011	21746946
spread of iscr1 elements containing bladha-1 and multiple antimicrobial resistance genes leading to increase of flomoxef resistance in esbl-producing klebsiella pneumoniae.	increasing resistance to quinolones, aminoglycosides and/or cephamycins in extended-spectrum ß-lactamase (esbl)-producing enterobacteriaceae exacerbates the already limited antibiotic treatment options for infections due to these microbes. in this study, the presence of resistance determinants for these antimicrobial agents was examined by pcr among esbl-producing klebsiella pneumoniae (esbl-kp) isolates that caused bacteremia. pulsed-field gel electrophoresis was used to differentiate the clona ...	2011	21746945
bacterial isolates of early-onset neonatal sepsis and their antibiotic susceptibility pattern between 1998 and 2004: an audit from a center in india.	abstract:	2011	21745376
rapid acquisition of decreased carbapenem susceptibility in a strain of klebsiella pneumoniae arising during meropenem therapy.	clin microbiol infect abstract: a strain of klebsiella pneumoniae (k1) was isolated from a catheterized patient with a urinary tract infection. the patient was subsequently treated with meropenem, after which k. pneumoniae (k2) was once again isolated from the patient's urine. susceptibility testing showed that strain k1 was fully susceptible to carbapenem antibiotics with the exception of ertapenem, to which it exhibited intermediate resistance, whilst k2 was resistant to ertapenem and meropene ...	2011	21745255
molecular analysis of population structure and antibiotic resistance of klebsiella isolates from a three-year surveillance program in florence hospitals, italy.	we report the results of a three-year surveillance program of klebsiella spp. in six hospitals in florence (italy). a total of 172 klebsiella isolates were identified and typed by aflp: 122 were k. pneumoniae and 50 were k. oxytoca. most k. pneumoniae (80%) and k. oxytoca (93%) showed unrelated aflp profiles. beside this heterogeneous population structure, we found five small epidemic clonal groups of k. pneumoniae. four of these groups were involved in outbreak events, three of which occurred i ...	2011	21744038
first report of a novel extended-spectrum beta-lactamase koxy-2 producing klebsiella oxytoca that hydrolyses cefotaxime and ceftazidime.	klebsiella oxytoca strains mu946294n and mb193997e were isolated from patients in scotland. strain mu946294n was resistant to pencillins, monbactams and cephalosporins. isolate mb193997e displayed a +¦-lactam resistance phenotype consistent with chromosomal +¦-lactamase overproduction. no bla(tem), bla(shv) or bla(ctx-m) genes could be amplified in either strain; however, amplification by pcr was found with primers for the bla(oxy-2) gene. this +¦-lactamase gene in mu946294n differed by one muta ...	2011	21742579
detection of volatile compounds produced by microbial growth in urine by selected ion flow tube mass spectrometry (sift-ms).	selected ion flow tube-mass spectrometry has been used to measure the volatile compounds occurring in the headspace of urine samples inoculated with common urinary tract infection (uti)-causing microbes escherichia coli, proteus vulgaris, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, klebsiella pneumoniae, enterococcus faecalis, or candida albicans. this technique has the potential to offer rapid and simple diagnosis of the causative agent of utis.	2011	21741416
a new class of 2-(4-cyanophenyl amino)-4-(6-bromo-4-quinolinyloxy)-6-piperazinyl (piperidinyl)-1,3,5-triazine analogues with antimicrobial/antimycobacterial activity.	this study presents the synthesis and in vitro pharmacological evaluations of novel 2-(4-cyanophenyl amino)-4-(6-bromo-4-quinolinyloxy)-6-piperazinyl (piperidinyl)-1,3,5-triazines. the title compounds were assayed for their in vitro antimicrobial activity against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneria) and four fungi (aspergillus niger, aspergillus fumigatus, a ...	2011	21740100
fecal carriage of serotype k1 klebsiella pneumoniae st23 strains closely related to liver abscess isolates in koreans living in korea.	we determined the fecal carriage rate of serotype k1 klebsiella pneumoniae in healthy koreans and studied their genetic relationship with liver abscess isolates. we compared the carriage according to the country of residence. the stool specimens were collected through health promotion programs in korea. k. pneumoniae strains were selected and tested for k1 by pcr. serotype k1 isolates were characterized by multilocus sequence typing and pulsed field gel electrophoresis. a total of 248ôçëk. pneum ...	2011	21739348
purple urine.		2011	21738253
[comparison of two phenotypic methods for detecting the production of extended-spectrum beta-lactamases by hospital klebsiella pneumoniae strains].	in the period 2006-2008, the republican emergence medical care center.isolated 2997 enterobacteria, 48.9% of which belonged to klebsiella pneumoniae. confirming phenotypic methods, such as double disk method (ddm) and double disk synergy test (ddst), were used to examine 64 k. pneumoniae strains that had been regarded as being suspected to produce extended-spectrum beta-lactamases (esbl) during a preliminary screening. of the 64 k. pneumoniae strains, 6 (9.4%) were unsusceptible to clavulanate a ...	2011	21735683
real-time detection of blakpc in clinical samples and surveillance specimens.	a real-time pcr assay was developed targeting the bla(kpc) responsible for klebsiella pneumoniae carbapenemase (kpc)-mediated carbapenem resistance and was validated for testing colonies or enrichment broth cultures. the assay accurately detects kpc-containing strains with high analytical specificity and sensitivity.	2011	21734030
establishment of the first international repository for transfusion-relevant bacteria reference strains: isbt working party transfusion-transmitted infectious diseases (wp-ttid), subgroup on bacteria.	backgroundôçé bacterial contamination of platelet concentrates (pcs) still remains a significant problem in transfusion with potential important clinical consequences, including death. the international society of blood transfusion working party on transfusion-transmitted infectious diseases, subgroup on bacteria, organised an international study on transfusion-relevant bacteria references to be used as a tool for development, validation and comparison of both bacterial screening and pathogen re ...	2011	21732948
antibiotic therapy in neonatal and pediatric septic shock.	severe sepsis accounts for nearly 4,500 deaths (mortality rate 10%), and is responsible for nearly $2 billion annual healthcare expenditure in the united states. early and speedy treatment of critically ill septic patients can halt or reduce the likelihood of physiologic progression to multi-system organ failure. a cornerstone of this therapeutic strategy is antibiotic administration. in this review, we discuss the empirical treatment strategies for the treatment of early and late neonatal sepsi ...	2011	21732046
aetiology of community-acquired urinary tract infection and antimicrobial susceptibility patterns of uropathogens isolated.	antibiotic resistance among uropathogens causing urinary tract infection (uti) is increasing worldwide. in most cases of uti, family physicians can provide empirical treatment without the benefit of a pre-therapy urine culture. knowledge of the aetiology and antimicrobial susceptibility patterns of uropathogens is important in order to determine the best empiric treatment option. this study aimed to determine the aetiology and antimicrobial susceptibility of uropathogens in culture-positive, com ...	2011	21731993
antimicrobial activity of essential oils of physalis angulata. l.	the need for a reduction in drug resistance led to the investigation of argemone mexicana l. as an agent against bacillus subtilis, klebsiella pneumoniae, staphylococcus aureus, pseudomonas aeruginosa, candida albicans, candida stellatoidea and candida torulopsis, using well diffusion and minimum inhibitory concentrations methods. the sensitivity of bacillus subtilis, klebsiella pneumoniae, pseudomonas aeruginosa and staphylococcus aureus to the essential oils of both the aerial and root parts w ...	2010	21731161
untreated primary thyroid lymphoma in an elderly woman.		2011	21727773
pharmacodynamics of meropenem in critically ill patients with febrile neutropenia and bacteraemia.	the bactericidal activity of β-lactams is determined by the time that concentrations in tissue and serum are above the minimum inhibitory concentration (t>mic) for the pathogen. the aim of this study was to compare the probability of target attainment (pta) and the cumulative fraction of response (cfr) for meropenem between administration by bolus injection and a 3-h infusion. the study was a randomised, three-way, cross-over design in eight febrile neutropenic patients with bacteraemia. each su ...	2011	21726984
diverse prevalence of 16s rrna methylase genes arma and rmtb amongst clinical multidrug-resistant escherichia coli and klebsiella pneumoniae isolates.	in this study, 112 escherichia coli and 55 klebsiella pneumoniae isolates with a multidrug-resistant (mdr) phenotype were collected from 2007 to 2009. all isolates simultaneously exhibited resistance to cefotaxime (or ceftazidime), ciprofloxacin (or levofloxacin) and amikacin. plasmid-mediated 16s rrna methylases, including arma, rmta, rmtb, rmtc, rmtd, rmte and npma, were detected by polymerase chain reaction (pcr) amplification. common +¦-lactamase genes, including bla(tem), bla(shv), bla(ctx- ...	2011	21724374
preliminary investigation of a mice model of klebsiella pneumoniae subsp. ozaenae induced pneumonia.	in the present study, we comparatively assessed the pathophysiological mechanisms developed during lung infection of balb/c female mice infected by an original wild type klebsiella pneumoniae subsp. ozaenae strain (ch137) or by a referent subspecies k. pneumoniae. subsp. pneumoniae strain (atcc10031). the mice infected with 2.10(6)-ácfu k. p. subsp. pneumoniae (n-á=-á10) showed transient signs of infection and all of them recovered. all of those infected with 1.10(6)-ácfu k. p. subsp. ozaenae (n ...	2011	21723409
klebsiella pneumoniae: development of a mixed population of carbapenem and tigecycline resistance during antimicrobial therapy in a kidney transplant patient.	clin microbiol infect abstract: nine isolates of klebsiella pneumoniae were isolated from a renal transplant patient suffering from recurrent urosepsis over a period of 4ôçâmonths. imipenem resistance was detected after imipenem-ertapenem therapy. when treatment was switched to tigecycline the k.-ápneumoniae developed resistance to tigecycline (micôçâ=ôçâ8ôçâmg/l). the nine isolates were tested by determination of agar dilution mics, phenotypic carbapenemase, extended-spectrum beta-lactamases an ...	2011	21722259
outcome of carbapenem resistant klebsiella pneumoniae bloodstream infections.	the aim of this study was to evaluate the impact of carbapenem-resistant k. pneumoniae bloodstream infections on mortality. during the study period 42, 68 and 120 patients were identified with carbapenem-resistant, extended-spectrum β-lactamase producers (esbl) and susceptible k. pneumoniae bloodstream infections, respectively. patients with carbapenem-resistant k. pneumoniae had higher rates of prior antimicrobial exposure, other nosocomial infections, and use of invasive devices. infection-rel ...	2011	21722257
a novel complex class 1 integron found in a klebsiella pneumoniae isolate from portugal.	klebsiella pneumoniae kp1 carrying a novel complex class 1 integron was isolated from an inanimate surface of a female ward sanitary facility in the hospital infante d. pedro, aveiro, central portugal. the integron consists of two variable regions (vrs); vr1 was previously described in escherichia coli and vibrio cholerae, and vr2 contains an in37-like structure and is located downstream of an iscr1 element. the integron was found on a plasmid of 225 kb. the qnrb10 gene, although present, is not ...	2011	21722254
extended-spectrum beta-lactamase-mediated resistance among bacterial isolates recovered from ocular infections.	purpose: to evaluate resistances mediated by extended-spectrum +¦-lactamases (esbls) and ampc +¦-lactamases among gram-negative bacteria recovered from ocular infections. methods: as per the clinical laboratory standards institute m100-s-16 document, a total of 135 gram-negative bacilli were recovered from ocular specimens and were subjected to phenotypic confirmation for esbl production by the double-disc synergy test, cephalosporin and clavulanate combination disc test and e test, and, for amp ...	2011	21720185
high mobility group nucleosomal binding domain 2 protein protects bladder epithelial cells from klebsiella pneumoniae invasion.	due to the predominance of multiple-antibiotic-resistant klebsiella pneumoniae strains, the search for new approaches for the prevention of k. pneumoniae infections is now under intensive investigation. the objective of the present study was to investigate the effects of high mobility group nucleosomal binding domain 2 (hmgn2) protein, which acts on the bladder epithelial cells t 24, on the invasion of k. pneumoniae 03183 and explore its possible mechanisms. pretreatment with hmgn2 significantly ...	2011	21720014
colonization of the gut with gram negative bacilli, its association with neonatal sepsis and its clinical relevance in a developing country.	this study examines the pattern of colonization of the neonatal gut by aerobic gram negative bacilli (gnb) and evaluates the association between gut colonization and sepsis in the developing world. this deserves attention because of the high incidence of sepsis and the differences in hygienic environment in developing countries compared to the developed world. the study was carried out on neonates in a tertiary care government hospital. the serial gut samples were analyzed (gastric aspirate, ga- ...	2011	21719575
[evolution of susceptibility to antibiotics of escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa and acinetobacter baumanii, in a university hospital center of beirut between 2005-áand 2009.]	introduction: until recently, multiresistant bacteria were only limited to hospitals. however, they are now responsible for community acquired infections, affecting people who have had no contact with the hospital environment. several mechanisms are associated with these resistances. the production of betalactamases is however the predominant mechanism and especially the production of extended spectrum beta-lactamases or esbl by strains of escherichia coli and klebsiella pneumoniae, which mediat ...	2011	21719212
antagonistic effect of lactobacillus strains against gas-producing coliforms isolated from colicky infants.	infantile colic is a common disturb within the first 3 months of life, nevertheless the pathogenesis is incompletely understood and treatment remains an open issue. intestinal gas production is thought to be one of the causes of abdominal discomfort in infants suffering from colic. however, data about the role of the amount of gas produced by infants' colonic microbiota and the correlation with the onset of colic symptoms are scanty. the benefit of supplementation with lactobacilli been recently ...	2011	21718486
molecular analysis of a prolonged spread of klebsiella pneumoniae co-producing dha-1 and shv-12 +¦-lactamases.	the study investigated molecular mechanisms for prolonged nosocomial spread of multidrug-resistant klebsiella pneumoniae co-producing plasmid-mediated ampc +¦-lactamase dha-1 and extended-spectrum +¦-lactamase shv-12. forty-eight clinical isolates of k. pneumonia, resistant to the extended-spectrum cepha-losporins, were collected in a 750-bed university hospital over a year. the isolates were characterized for pcr-based +¦-lactamase genotypes, isoelectric focusing and pulsed-field gel electropho ...	2011	21717319
usefulness of c-reactive protein for evaluating clinical outcomes in cirrhotic patients with bacteremia.	the purpose of this study was to evaluate the value of initial c-reactive protein (crp) as a predictor of clinical outcome and to investigate whether follow-up crp measurement is useful for the prediction of the clinical outcome of bloodstream infections in patients with liver cirrhosis (lc), whose crp production in response to infection may be attenuated.	2011	21716910
contemporary activity of colistin and polymyxin b against a worldwide collection of gram-negative pathogens: results from the sentry antimicrobial surveillance program (2006-09).	objectives to comparatively evaluate the antimicrobial activities of colistin and polymyxin b with those of other antimicrobials against a worldwide collection of 40ôçè625 gram-negative bacilli. methods antimicrobial susceptibility testing was performed and interpreted using the clsi broth microdilution method except for colistin against enterobacteriaceae. results the polymyxins showed potent in vitro activities (mic(90), ôëñ0.5-1 mg/l) against this large collection of clinical isolates, with v ...	2011	21715434
inflammation restraining effects of prostaglandin e2 on natural killer-dendritic cell (nk-dc) interaction are imprinted during dc maturation.	among prostaglandins (pgs), pge2 is abundantly expressed in various malignancies and is probably one of many factors promoting tumor growth by inhibiting tumor immune surveillance(.) in the current study, we report on a novel mechanism by which pge2 inhibits in vitro natural killer-dendritic cell (nk-dc) crosstalk and thereby innate and adaptive immune responses via its effect on nk-dc crosstalk. the presence of pge2 during ifn-+¦/membrane fraction of klebsiella pneumoniae dc maturation inhibits ...	2011	21715307
physicochemical and microbiological assessment of recreational and drinking waters.	the present study was aimed to make an assessment of health risk due to pollution and human pathogenic bacteria associated with the recreational and drinking water sources in twin densely populated holy indian cities ayodhya and faizabad. though physicochemical studies revealed that the water available in the area is under recommended limits for human use, it is unsafe on account of poor microbiological quality of surface and ground water in the region. the most probable number (mpn) test result ...	2011	21713494
[antimicrobial resistance of gram negative bacilli isolated from terciary-care hospitals in colombia].	antimicrobial resistance has been identified as one of the major public health problems worldwide. to facilitate its control, bacterial resistance levels must be monitored permanently by effective surveillance systems.	2010	21713339
evaluations of bacterial contaminated full thickness burn wound healing in sprague dawley rats treated with tualang honey.	the effect of tualang honey on wound healing in bacterial contaminated full-thickness burn wounds was evaluated in 36 male sprague dawley rats.	2011	21713196
changes in acute-phase proteins and cytokines in serum and milk whey from dairy cows with naturally occurring peracute mastitis caused by klebsiella pneumoniae and the relationship to clinical outcome.	this study was conducted to evaluate the changes in acute-phase proteins and cytokine concentrations in dairy cows with naturally occurring peracute klebsiella pneumoniae (k. pneumoniae) mastitis and their association with the outcome of the disease. seventeen holstein cows with k. pneumoniae mastitis from 8 dairy farms were divided on the basis of outcome after local and systemic therapy into 2 groups comprising 8 euthanized cows and 9 that recovered. changes in acute-phase proteins and cytokin ...	2011	21712644
comparison of ctx-m-14- and ctx-m-15-producing escherichia coli and klebsiella pneumoniae isolates from patients with bacteremia.	recently, ctx-m-15-producing enterobacteriaceae has disseminated worldwide. to better understand the success of ctx-m-15-type extended-spectrum +¦-lactamase, we compared the ctx-m-15-producing escherichia coli and klebsiella pneumoniae isolates with ctx-m-14-producing e. coli and k. pneumoniae isolates that had been more prevalent before the recent increase of ctx-m-15 in korea.	2011	21712135
antimicrobial resistance patterns of uropathogens among children in istanbul, turkey.	urinary tract infections are a common cause of end-stage renal disease in turkey. this prospective study investigated the antibiotic resistance patterns of uropathogens in order to recommend appropriate therapeutic protocols for children with urinary tract infections in istanbul, turkey. between october 2007 and october 2008, children presenting with a first episode of urinary tract infection to a pediatric outpatient clinic were enrolled in the study. urine samples were cultured, and antimicrob ...	2011	21710858
changes in bacterial resistance patterns in children with urinary tract infections on antimicrobial prophylaxis at university hospital in split.	we assessed prevalence and resistance of uropathogens on antimicrobial agents (aa) from urine cultures (uc) in children hospitalized with urinary tract infections (uti) at university hospital in split.	2011	21709628
pus in the ventricles of a patient with a lumbar cerebrospinal fluid drain for a thoracoabdominal aneurysm repair.		2011	21709541
in vitro potency of various polymyxin b components.		2011	21709096
in vitro spectrum of activity of finafloxacin, a novel, ph-activated fluoroquinolone, under standard and acidic conditions.	finafloxacin is a novel fluoroquinolone that exhibits enhanced antibacterial activity under acidic conditions. the aim of this study was to define the in vitro ph-activity relationship. finafloxacin exhibited optimal antibacterial activity between ph 5.0 and 6.0 at which mics were 4- to 8-fold lower than those determined at neutral ph. these observations were then confirmed against a larger collection of bacteria. these data suggest that finafloxacin could potentially offer a therapeutic advanta ...	2011	21709094
analysis of the networks controlling the antimicrobial-peptide-dependent induction of klebsiella pneumoniae virulence factors.	antimicrobial peptides (aps) impose a threat to the survival of pathogens, and it is reasonable to postulate that bacteria have developed strategies to counteract them. polymyxins are becoming the last resort to treat infections caused by multidrug-resistant gram-negative bacteria and, similar to aps, they interact with the anionic lipopolysaccharide. given that polymyxins and aps share the initial target, it is possible that bacterial defense mechanisms against polymyxins will be also effective ...	2011	21708987
arthritis, osteomyelitis, septicemia and meningitis caused by klebsiella in a low-birth-weight newborn: a case report.	abstract:	2011	21707974
bacterial contamination of saudi "one" riyal paper notes.	fomites are inanimate objects that are capable of absorbing, harboring and transmitting infectious microorganisms. paper currency, an exchangeable fomite, is constantly subjected to contamination. this study investigated bacterial contamination on the saudi one riyal paper note. two hundred one riyal bills (100 4th version and 100 5th version) were collected in the city of jeddah for examination. the notes were examined for bacterial contamination using blood agar and macconkey agar. eighty-eigh ...	2011	21706951
cloning, expression, and enzymatic activity of acinetobacter baumannii and klebsiella pneumoniae acetyl-coenzyme a carboxylases.	pathogenic gram-negative bacteria are a major public health concern because they are causative agents of life-threatening hospital-acquired infections. due to the increasing rates of resistance to available antibiotics, there is an urgent need to develop new drugs. acetyl-coenzyme a carboxylase (accase) is a promising target for the development of novel antibiotics. we describe here the expression, purification, and enzymatic activity of recombinant accases from two clinically relevant gram-nega ...	2011	21704013
molecular detection and epidemiology of extended-spectrum beta-lactamase genes prevalent in clinical isolates of klebsiella pneumoniae and e coli from trinidad and tobago.	the epidemiology of extended-spectrum beta-lactamase (esbl) producing e coli and k pneumoniae is complex and varies among hospitals and countries. this study aimed at describing the molecular detection and epidemiology of esbl subtypes prevalent in clinical isolates of k pneumonia and e coli in trinidad and tobago.	2010	21702229
horizontal transfer of antimicrobial resistance by extended-spectrum +¦ lactamase-producing enterobacteriaceae.	the purpose of this work was to study the acquisition of new antibiotic-resistant genes carried by extended spectrum +¦-lactamase (esbl)-producing enterobacteriaceae via horizontal transfer to understand their rampant spread in the hospitals and in the community.	2011	21701662
lateral buttock congenital dermal sinus tract.	a 6-month-old female presented with purulent discharge from a dimple in the right lateral buttock. a subcutaneous abscess was palpated on the right paravertebral region at the l5-s1 level. she had low-grade fever with laboratory findings of leukocytosis and elevation of c-reactive protein levels. klebsiella and enterococcus species were cultured from the pus. computed tomography (ct) clearly showed a tract traversing the subcutaneous tissue and connecting to the abscess. magnetic resonance (mr) ...	2011	21701114
factors associated with the quality of well water and the prevalence of waterborne diseases in the municipality of abomey-calavi in benin.	the objective of this work is to assess the factors associated with the water quality of household wells, suggest solutions to improve it, and study the prevalence of water-borne diseases in this community. the quality of well water and the prevalence of waterborne diseases in the municipality were studied by analysis of the microbiological indicators currently used to assess drinking-water quality and the retrospective study of waterborne diseases treated in the local health centres. the wells ...	2011	21700557
risk factors associated with short-term post-treatment outcomes of clinical mastitis.	the objectives of this study were to characterize 60-d outcomes after treatment of mild (abnormal milk) and moderate (abnormal milk and abnormal udder) cases of clinical mastitis (cm) occurring in a single quarter of cows on wisconsin farms (n=4) and to determine risk factors associated with those outcomes. duplicate milk samples were collected from the affected quarter of each cow for microbiological analysis at the onset of cm (pre) and 21 d later (post). cows were treated only in the affected ...	2011	21700025
an optimized method for suicide vector-based allelic exchange in klebsiella pneumoniae.	klebsiella pneumoniae is an important and versatile bacterium that can be found in diverse environments and is also a frequent cause of human infections. limited data exists on the mechanisms of interaction between k. pneumoniae and the human host and of adaptations to other environments. coupled with the high genetic diversity of this species, these factors highlight the necessity for substantial further k. pneumoniae-focused molecular genetics studies. in this report we describe a simple and e ...	2011	21699924
antimicrobial activity of liposome encapsulated cyclo(l-tyrosyl-l-prolyl).	various studies have shown the potentially beneficial biological activities of cyclic dipeptides and in particular, cyclo(l-tyrosyl-l-prolyl) (cyclo(tyr-pro)) has shown fair antibacterial activity in vitro. this study aimed to determine if liposome encapsulation would have any significant effects on the antibacterial activity of this compound. the thin-film hydration method with extrusion was used to produce small unilamellar vesicles containing cyclo(tyr-pro) that were shown to have an average ...	2011	21699080
bacterial community composition of biological degreasing systems and health risk assessment for workers.	biological degreasing system is a new technology based on the degradation capabilities of microorganisms to remove oil, grease, or lubricants from metal parts. no data is available about the potential biological health hazards in such system. thus, a health risk assessment linked to the bacterial populations present in this new degreasing technology is, therefore, necessary for workers. we performed both cultural and molecular approaches in several biological degreasing systems for various indus ...	2011	21698403