Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| progress in genetic engineering of peanut (arachis hypogaea l.)--a review. | peanut (arachis hypogaea l.) is a major species of the family, leguminosae, and economically important not only for vegetable oil but as a source of proteins, minerals and vitamins. it is widely grown in the semi-arid tropics and plays a role in the world agricultural economy. peanut production and productivity is constrained by several biotic (insect pests and diseases) and abiotic (drought, salinity, water logging and temperature aberrations) stresses, as a result of which crop experiences ser ... | 2015 | 25626474 |
| regulation of oncogene expression in t-dna-transformed host plant cells. | virulent agrobacterium tumefaciens strains integrate their t-dna into the plant genome where the encoded agrobacterial oncogenes are expressed and cause crown gall disease. essential for crown gall development are iaah (indole-3-acetamide hydrolase), iaam (tryptophan monooxygenase) and ipt (isopentenyl transferase), which encode enzymes for the biosynthesis of auxin (iaah, iaam) and cytokinin (ipt). although these oncogenes are well studied as the tumor-inducing principle, nothing is known about ... | 2015 | 25615824 |
| the phylogenetically-related pattern recognition receptors efr and xa21 recruit similar immune signaling components in monocots and dicots. | during plant immunity, surface-localized pattern recognition receptors (prrs) recognize pathogen-associated molecular patterns (pamps). the transfer of prrs between plant species is a promising strategy for engineering broad-spectrum disease resistance. thus, there is a great interest in understanding the mechanisms of prr-mediated resistance across different plant species. two well-characterized plant prrs are the leucine-rich repeat receptor kinases (lrr-rks) efr and xa21 from arabidopsis thal ... | 2015 | 25607985 |
| enhanced-transient expression of hepatitis c virus core protein in nicotiana tabacum, a protein with potential clinical applications. | hepatitis c virus (hcv) is major cause of liver cirrhosis in humans. hcv capsid (core) protein (hcvcp) is a highly demanded antigen for various diagnostic, immunization and pathogenesis studies. plants are considered as an expression system for producing safe and inexpensive biopharmaceutical proteins. although invention of transgenic (stable) tobacco plants expressing hcvcp with proper antigenic properties was recently reported, no data for "transient-expression" that is currently the method of ... | 2014 | 25598788 |
| snare-rnai results in higher terpene emission from ectopically expressed caryophyllene synthase in nicotiana benthamiana. | plants produce numerous terpenes and much effort has been dedicated to the identification and characterization of the terpene biosynthetic genes. however, little is known about how terpenes are transported within the cell and from the cell into the apoplast. to investigate a putative role of vesicle fusion in this process, we used agrobacterium tumefaciens-mediated transient coexpression in nicotiana benthamiana of an mtvamp721e-rnai construct (vi) with either a caryophyllene synthase or a linal ... | 2015 | 25598143 |
| genomic analyses of metal resistance genes in three plant growth promoting bacteria of legume plants in northwest mine tailings, china. | to better understand the diversity of metal resistance genetic determinant from microbes that survived at metal tailings in northwest of china, a highly elevated level of heavy metal containing region, genomic analyses was conducted using genome sequence of three native metal-resistant plant growth promoting bacteria (pgpb). it shows that: mesorhizobium amorphae ccnwgs0123 contains metal transporters from p-type atpase, cdf (cation diffusion facilitator), hupe/urej and chr (chromate ion transpor ... | 2015 | 25597676 |
| the agrobacterium ti plasmids. | agrobacterium tumefaciens is a plant pathogen with the capacity to deliver a segment of oncogenic dna carried on a large plasmid called the tumor-inducing or ti plasmid to susceptible plant cells. a. tumefaciens belongs to the class alphaproteobacteria, whose members include other plant pathogens (agrobacterium rhizogenes), plant and insect symbionts (rhizobium spp. and wolbachia spp., respectively), human pathogens (brucella spp., bartonella spp., rickettsia spp.), and nonpathogens (caulobacter ... | 2014 | 25593788 |
| chemical composition and antibacterial activity of methanolic extract and essential oil of iranian teucrium polium against some of phytobacteria. | the antibacterial activity of essential oil and methanolic extract of teucrium polium was determined against pseudomonas aeruginosa, pantoea agglomerans, brenneria nigrifluens, rhizobium radiobacter, rhizobium vitis, streptomyces scabies, ralstonia solanacearum, xanthomonas campestris and pectobacterium cartovorum by disc diffusion method. minimum inhibitory concentration and minimum bactericidal concentration were determined by using the serial dilution method. chemical composition of essential ... | 2015 | 25583240 |
| triesterase and promiscuous diesterase activities of a di-co(ii)-containing organophosphate degrading enzyme reaction mechanisms. | the reaction mechanism for the hydrolysis of trimethyl phosphate and of the obtained phosphodiester by the di-co(ii) derivative of organophosphate degrading enzyme from agrobacterium radiobacter p230(opda), have been investigated at density functional level of theory in the framework of the cluster model approach. both mechanisms proceed by a multistep sequence and each catalytic cycle begins with the nucleophilic attack by a metal-bound hydroxide on the phosphorus atom of the substrate, leading ... | 2015 | 25582757 |
| simple screening strategy with only water bath needed for the identification of insect-resistant genetically modified rice. | an informative, with simple instrument needed, rapid and easily updated strategy for the identification of insect-resistant genetically modified (gm) rice has been described. such strategy is based on a parallel series of loop-mediated isothermal amplification (lamp) reactions targeting the rice endogenous gene sucrose phosphate synthase (sps), the top two most frequently used genetic elements (agrobacterium tumefaciens nopaline synthase terminator (nos) and cauliflower mosaic virus 35s promoter ... | 2015 | 25582220 |
| arsenite oxidase also functions as an antimonite oxidase. | arsenic and antimony are toxic metalloids and are considered priority environmental pollutants by the u.s. environmental protection agency. significant advances have been made in understanding microbe-arsenic interactions and how they influence arsenic redox speciation in the environment. however, even the most basic features of how and why a microorganism detects and reacts to antimony remain poorly understood. previous work with agrobacterium tumefaciens strain 5a concluded that oxidation of a ... | 2015 | 25576601 |
| isolation and characterization of a secoiridoid derivative from two-phase olive waste (alperujo). | a secoiridoid derivative was isolated from the ethyl acetate extract of two-phase olive waste (alperujo). the structure of this compound was fully characterized as s-trans-(e)-3-(1-oxobut-2-en-2-yl)glutaric acid. the spectroscopic data, including one- and two-dimensional nuclear magnetic resonance, mass spectrometry, infrared analysis, and ultraviolet spectrum, were showed. the origin of this compound has not been previously studied, although it most likely results from the breakdown of the oleu ... | 2015 | 25574580 |
| cloning and expression of tnf related apoptosis inducing ligand in nicotiana tabacum. | molecular farming has been considered as a secure and economical approach for production of biopharmaceuticals. human tnf related apoptosis inducing ligand (trail) as a promising biopharmaceutical candidate has been produced in different expression hosts. however, little attention has been paid to molecular farming of the trail in spite of numerous advantages of plant expression systems. therefore, in this study the cytoplasmic production of the trail was tackled in nicotiana tabacum using agrob ... | 2015 | 25561925 |
| effect of agrobacterium strain and plasmid copy number on transformation frequency, event quality and usable event quality in an elite maize cultivar. | improving agrobacterium -mediated transformation frequency and event quality by increasing binary plasmid copy number and appropriate strain selection is reported in an elite maize cultivar. agrobacterium-mediated maize transformation is a well-established method for gene testing and for introducing useful traits in a commercial biotech product pipeline. to develop a highly efficient maize transformation system, we investigated the effect of two agrobacterium tumefaciens strains and three differ ... | 2015 | 25558819 |
| plant-agrobacterium interaction mediated by ethylene and super-agrobacterium conferring efficient gene transfer. | agrobacterium tumefaciens has a unique ability to transfer genes into plant genomes. this ability has been utilized for plant genetic engineering. however, the efficiency is not sufficient for all plant species. several studies have shown that ethylene decreased the agrobacterium-mediated transformation frequency. thus, a. tumefaciens with an ability to suppress ethylene evolution would increase the efficiency of agrobacterium-mediated transformation. some studies showed that plant growth-promot ... | 2014 | 25520733 |
| peritonitis in a peritoneal dialysis patient due to rhizobium radiobacter and moraxella osleonsis: case report and literature review. | 2017 | 25520491 | |
| interaction of the agrobacterium tumefaciens virulence protein vird2 with histones. | agrobacterium tumefaciens is a gram-negative soil bacterium that genetically transforms plants and, under laboratory conditions, also transforms non-plant organisms, such as fungi and yeasts. during the transformation process a piece of ssdna (t-strand) is transferred into the host cells via a type iv secretion system. the vird2 relaxase protein, which is covalently attached at the 5' end of the t-strand through tyr29, mediates nuclear entry as it contains a nuclear localization sequence. how th ... | 2015 | 25505187 |
| an efficient plant regeneration and agrobacterium-mediated genetic transformation of tagetes erecta. | tagetes erecta, l. an asteraceous plant of industrial and medicinal value, contains important compounds like pyrethrins, thiophenes and lutein, possessing immense potential for insecticidal, nematicidal and nutraceutical activities. considering the importance and demand for these natural compounds, genetic manipulation of this crop for better productivity of secondary metabolites holds great significance. a rapid and reproducible direct regeneration and genetic transformation system is the prere ... | 2015 | 25504508 |
| insights into the polerovirus-plant interactome revealed by coimmunoprecipitation and mass spectrometry. | identification of host proteins interacting with the aphidborne potato leafroll virus (plrv) from the genus polerovirus, family luteoviridae, is a critical step toward understanding how plrv and related viruses infect plants. however, the tight spatial distribution of plrv to phloem tissues poses challenges. a polyclonal antibody raised against purified plrv virions was used to coimmunoprecipitate virus-host protein complexes from nicotiana benthamiana tissue inoculated with an infectious plrv c ... | 2015 | 25496593 |
| a rapid and stable agrobacterium-mediated transformation method of a medicinal plant chelone glabra l. | transformation approach is a useful tool for the study of gene function, the mechanism of molecular regulation, and increase usefulness of components by reverse genetic approach in plants. in this study, we developed a stable and rapid method for agrobacterium-mediated transformation of a medicinal plant chelone glabra l. using leaf explants. stable transformants were obtained using agrobacterium tumefaciens strains gv2260 and gv3101 that harbored the binary vector pbi121 and contained the neomy ... | 2015 | 25492686 |
| overexpression of the pumpkin (cucurbita maxima) 16 kda phloem protein cmpp16 increases tolerance to water deficit. | the phloem plays an important role in the delivery of nutrients and signals between photosynthetic to heterotrophic tissues. proteins and rnas in the phloem translocation stream may have an important role in maintaining the integrity of the sieve tube system, as well as in long-distance signaling. cmpp16 is a pumpkin phloem protein, which has been shown to bind rna in a non-sequence specific manner, and move it cell-to-cell and conceivably, long-distance. the protein and rna are found in both co ... | 2014 | 25482781 |
| application of sonication in combination with vacuum infiltration enhances the agrobacterium-mediated genetic transformation in indian soybean cultivars. | soybean is a recalcitrant crop to agrobacterium-mediated genetic transformation. development of highly efficient, reproducible, and genotype-independent transformation protocol is highly desirable for soybean genetic improvement. hence, an improved agrobacterium-mediated genetic transformation protocol has been developed for cultivar pk 416 by evaluating various parameters including agrobacterium tumefaciens strains (lba4404, eha101, and eha105 harboring pcambia1304 plasmid), sonication duration ... | 2015 | 25480345 |
| characterisation and microstructure of reduced-fat chicken patties made with a novel polymer from agrobacterium radiobacter k84. | chicken patties elaborated with a novel polymer from agrobacterium radiobacter k84 (arb) were characterised during 60days of frozen storage. after cooking, formulations without arb (f0), with arb 5 g/100 g (f5) and arb 10 g/100 g (f10) presented 4.23%, 2.83% and 0.11% fat, respectively. no differences were observed to water holding capacity, cooking yield and shear force among formulations. microstructural analysis showed formation of meat emulsion for f5 and gel for f10. colour and chicken flav ... | 2015 | 25466137 |
| phylogenetic multilocus sequence analysis of native rhizobia nodulating faba bean (vicia faba l.) in egypt. | the taxonomic diversity of forty-two rhizobium strains, isolated from nodules of faba bean grown in egypt, was studied using 16s rrna sequencing, multilocus sequence analyses (mlsa) of three chromosomal housekeeping loci and one nodulation gene (noda). based on the 16s rrna gene sequences, most of the strains were related to rhizobium leguminosarum, rhizobium etli, and rhizobium radiobacter (syn. agrobacterium tumefaciens). a maximum likelihood (ml) tree built from the concatenated sequences of ... | 2014 | 25458609 |
| structure and function of a decarboxylating agrobacterium tumefaciens keto-deoxy-d-galactarate dehydratase. | agrobacterium tumefaciens (at) strain c58 contains an oxidative enzyme pathway that can function on both d-glucuronic and d-galacturonic acid. the corresponding gene coding for at keto-deoxy-d-galactarate (kdg) dehydratase is located in the same gene cluster as those coding for uronate dehydrogenase (at udh) and galactarolactone cycloisomerase (at gci) which we have previously characterized. here, we present the kinetic characterization and crystal structure of at kdg dehydratase, which catalyze ... | 2014 | 25454257 |
| ecological dynamics and complex interactions of agrobacterium megaplasmids. | as with many pathogenic bacteria, agrobacterial plant pathogens carry most of their virulence functions on a horizontally transmissible genetic element. the tumor-inducing (ti) plasmid encodes the majority of virulence functions for the crown gall agent agrobacterium tumefaciens. this includes the vir genes which drive genetic transformation of host cells and the catabolic genes needed to utilize the opines produced by infected plants. the ti plasmid also encodes, an opine-dependent quorum sensi ... | 2014 | 25452760 |
| dc-analyzer-facilitated combinatorial strategy for rapid directed evolution of functional enzymes with multiple mutagenesis sites. | iterative saturation mutagenesis (ism) has been shown to be a powerful method for directed evolution. in this study, the approach was modified (termed m-ism) by combining the single-site saturation mutagenesis method with a dc-analyzer-facilitated combinatorial strategy, aiming to evolve novel biocatalysts efficiently in the case where multiple sites are targeted simultaneously. initially, all target sites were explored individually by constructing single-site saturation mutagenesis libraries. n ... | 2014 | 25449543 |
| c-terminal domain of cagx is responsible for its interaction with cagt protein of helicobacter pylori type iv secretion system. | helicobacter pylori are the well known human pathogen associated with gastric cancer and peptic ulcer. pathogenesis is mainly due to the presence of 40 kb cagpai (cag pathogenicity island) region that encodes the type iv secretion system (tfss) consisting of a cytoplasmic part, a middle part/core complex (spans from inner membrane to outer membrane), and an outer membrane associated part. cagx and cagt are two important proteins of tfss that have homology with virb9 and virb7 of agrobacterium tu ... | 2015 | 25446105 |
| development and validation of a p-35s, t-nos, t-35s and p-fmv tetraplex real-time pcr screening method to detect regulatory genes of genetically modified organisms in food. | in routine analysis screening methods based on real-time pcr (polymerase chain reaction) are most commonly used for the detection of genetically modified (gm) plant material in food and feed. screening tests are based on sequences frequently used for gm development, allowing the detection of a large number of gmos (genetically modified organisms). here, we describe the development and validation of a tetraplex real-time pcr screening assay comprising detection systems for the regulatory genes ca ... | 2014 | 25437161 |
| overexpression of bacterial mtld gene in peanut improves drought tolerance through accumulation of mannitol. | in the changing global environmental scenarios, water scarcity and recurrent drought impose huge reductions to the peanut (arachis hypogaea l.) crop yield. in plants, osmotic adjustments associated with efficient free radical scavenging ability during abiotic stress are important components of stress tolerance mechanisms. mannitol, a compatible solute, is known to scavenge hydroxyl radicals generated during various abiotic stresses, thereby conferring tolerance to water-deficit stress in many pl ... | 2014 | 25436223 |
| novel compounds that enhance agrobacterium-mediated plant transformation by mitigating oxidative stress. | agrobacterium tumefaciens caused tissue browning leading to subsequent cell death in plant transformation and novel anti-oxidative compounds enhanced agrobacterium -mediated plant transformation by mitigating oxidative stress. browning and death of cells transformed with agrobacterium tumefaciens is a long-standing and high impact problem in plant transformation and the agricultural biotechnology industry, severely limiting the production of transgenic plants. using our tomato cv. microtom trans ... | 2015 | 25429877 |
| progress of cereal transformation technology mediated by agrobacterium tumefaciens. | monocotyledonous plants were believed to be not transformable by the soil bacterium agrobacterium tumefaciens until two decades ago, although convenient protocols for infection of leaf disks and subsequent regeneration of transgenic plants had been well established in a number of dicotyledonous species by then. this belief was reinforced by the fact that monocotyledons are mostly outside the host range of crown gall disease caused by the bacterium and by the failures in trials in monocotyledons ... | 2014 | 25426132 |
| blocking single-stranded transferred dna conversion to double-stranded intermediates by overexpression of yeast dna replication factor a. | agrobacterium tumefaciens delivers its single-stranded transferred dna (t-strand) into the host cell nucleus, where it can be converted into double-stranded molecules. various studies have revealed that double-stranded transfer dna (t-dna) intermediates can serve as substrates by as yet uncharacterized integration machinery. nevertheless, the possibility that t-strands are themselves substrates for integration cannot be ruled out. we attempted to block the conversion of t-strands into double-str ... | 2015 | 25424309 |
| efficient transformation and expression of gfp gene in valsa mali var. mali. | valsa mali var. mali, the causal agent of valsa canker of apple, causes great loss of apple production in apple producing regions. the pathogenic mechanism of the pathogen has not been studied extensively, thus a suitable gene marker for pathogenic invasion analysis and a random insertion of t-dna for mutants are desirable. in this paper, we reported the construction of a binary vector pko1-hph containing a positive selective gene hygromycin phosphotransferase (hph), a reporter gene gfp conferri ... | 2015 | 25423905 |
| global and phylogenetic distribution of quorum sensing signals, acyl homoserine lactones, in the family of vibrionaceae. | bacterial quorum sensing (qs) and the corresponding signals, acyl homoserine lactones (ahls), were first described for a luminescent vibrio species. since then, detailed knowledge has been gained on the functional level of qs; however, the abundance of ahls in the family of vibrionaceae in the environment has remained unclear. three hundred and one vibrionaceae strains were collected on a global research cruise and the prevalence and profile of ahl signals in this global collection were determin ... | 2014 | 25419995 |
| construction of infectious clones of tomato torrado virus and their delivery by agroinfiltration. | the first biologically active infectious clones of tomato torrado virus (totv) were generated and delivered into nicotiana benthamiana and solanum lycopersicum plants via agrobacterium tumefaciens. the engineered constructs consisted of pcr-amplified complementary dnas derived from the totv rna1 and rna2 components, individually inserted into an engineered pgreen binary vector between the camv 35s promoter and nopaline synthase terminator. these constructs were introduced into the plant hosts by ... | 2015 | 25416854 |
| tall fescue (festuca arundinacea schreb.). | tall fescue (festuca arundinacea schreb.) is the predominant cool-season perennial grass in the united states. it is widely used for both forage and turf purposes. this chapter describes a protocol that allows for the generation of a large number of transgenic tall fescue plants by agrobacterium tumefaciens-mediated transformation. embryogenic calli induced from caryopsis are used as explants for inoculation with a. tumefaciens. the agrobacterium strain used is eha105. hygromycin phosphotransfer ... | 2015 | 25416272 |
| poinsettia (euphorbia pulcherrima willd. ex klotzsch). | genetic engineering is an important tool for introducing desired genes into poinsettia (euphorbia pulcherrima willd. ex klotzsch). we describe in this chapter an agrobacterium tumefaciens-mediated transformation protocol for poinsettia. a detailed description of genetic transformation, antibiotic selection, subsequent regeneration via somatic embryogenesis, and rooting as well as molecular and morphological analyses is included. the methodology described here could facilitate the future engineer ... | 2015 | 25416270 |
| hemp (cannabis sativa l.). | hemp (cannabis sativa l.) suspension culture cells were transformed with agrobacterium tumefaciens strain eha101 carrying the binary plasmid pnov3635. the plasmid contains a phosphomannose isomerase (pmi) selectable marker gene. cells transformed with pmi are capable of metabolizing the selective agent mannose, whereas cells not expressing the gene are incapable of using the carbon source and will stop growing. callus masses proliferating on selection medium were screened for pmi expression usin ... | 2015 | 25416268 |
| sugarcane (saccharum spp. hybrids). | genetic transformation of sugarcane has a tremendous potential to complement traditional breeding in crop improvement and will likely transform sugarcane into a bio-factory for value-added products. we describe here agrobacterium tumefaciens-mediated transformation of sugarcane. embryogenic callus induced from immature leaf whorls was used as target for transformation with the hypervirulent agrobacterium strain agl1 carrying a constitutive nptii expression cassette in vector ppzp200. selection w ... | 2015 | 25416267 |
| peach (prunus persica l.). | until now, the application of genetic transformation techniques in peach has been limited by the difficulties in developing efficient regeneration and transformation protocols. here we describe an efficient regeneration protocol for the commercial micropropagation of gf677 rootstock (prunus persica × prunus amygdalus). the method is based on the production, via organogenesis, of meristematic bulk tissues characterized by a high competence for shoot regeneration. this protocol has also been used ... | 2015 | 25416260 |
| cherry. | agrobacterium tumefaciens-mediated transformation of sour chgahtvy (prunus cerasus l.) "montmorency" and sweet cherry rootstocks "gisela 6" and "gisela 7" (p. cerasus × p. canescens) is described. briefly, leaf explants from in vitro shoots are cocultivated with a. tumefaciens either directly (for "gisela 6" and "gisela 7") or after pretreatment (for "montmorency") on cocultivation medium; selection and regeneration of transformed shoots are carried out on selection medium containing 50 mg/l kan ... | 2015 | 25416255 |
| blueberry (vaccinium corymbosum l.). | vaccinium consists of approximately 450 species, of which highbush blueberry (vaccinium corymbosum) is one of the three major vaccinium fruit crops (i.e., blueberry, cranberry, and lingonberry) domesticated in the twentieth century. in blueberry the adventitious shoot regeneration using leaf explants has been the most desirable regeneration system to date; agrobacterium tumefaciens-mediated transformation is the major gene delivery method and effective selection has been reported using either th ... | 2015 | 25416254 |
| taro (colocasia esculenta (l.) schott). | genetic engineering of taro is an effective method to improve taro quality and the resistance to various diseases of taro. agrobacterium tumefaciens-mediated transformation of taro is more efficient than the particle bombardment transformation method based on current research. the development of a regeneration system starting from taro shoot tip explants could produce dasheen mosaic virus (dsmv)-free plantlets. highly regenerative calluses could be developed from dsmv-free, in vitro plantlets on ... | 2015 | 25416252 |
| carrot (daucus carota l.). | plants are susceptible to infection by a broad range of fungal pathogens. a range of proteins have been evaluated that can enhance tolerance to these pathogens by heterologous expression in transgenic carrot tissues. the protocols for carrot transformation with arabidopsis npr1 (non-expressor of pathogenesis-related proteins 1) are described in this chapter, using the herbicide resistance gene bar, which encodes phosphinothricin acetyltransferase, as a selectable marker. in this protocol, petiol ... | 2015 | 25416249 |
| sunflower (helianthus annuus l.). | sunflower (helianthus annuus l.) is still considered as a recalcitrant species to in vitro culture and transformation in spite of the publication of different protocols. here we describe a routine transformation system of this crop which requires mature ha89 genotype seeds and agrobacterium tumefaciens eha105 strain for gene delivery, being both easily available. selection of transformed shoots depends on root development in kanamycin-selective media, instead of shoot color, avoiding selection o ... | 2015 | 25416248 |
| jatropha (jatropha curcas l.). | the seed oil of jatropha (jatropha curcas l.) as a source of biodiesel fuel is gaining worldwide importance. commercial-scale exploration of jatropha has not succeeded due to low and unstable seed yield in semiarid lands unsuitable for the food production and infestation to diseases. genetic engineering is promising to improve various agronomic traits in jatropha and to understand the molecular functions of key jatropha genes for molecular breeding. we describe a protocol routinely followed in o ... | 2015 | 25416246 |
| genetic transformation of the tomato pathogen pyrenochaeta lycopersici allowed gene knockout using a split-marker approach. | pyrenochaeta lycopersici, as other soil-transmitted fungal pathogens, generally received little attention compared to the pathogens affecting the aerial parts of the plants, although causing stunt and important fruit yield reduction of agronomic relevant crops. the scope of this study was to develop a system allowing to investigate the functional role of p. lycopersici genes putatively involved in the corky root rot of tomato. a genetic transformation system based on a split-marker approach was ... | 2015 | 25413737 |
| root and shoot parts of strawberry: factories for production of functional human pro-insulin. | diabetes, a disease caused by excessive blood sugar, is caused by the lack of insulin. for commercial production, insulin is made in bacteria or yeast by protein recombinant technology. the focus of this research is evaluating another resource and producing of recombinant insulin protein in as strawberry as this plant has high potential in production of pharmaceutical proteins. strawberry is a suitable bioreactor for production of recombinant proteins especially edible vaccines. in this research ... | 2015 | 25403333 |
| membrane-binding mechanism of a bacterial phospholipid n-methyltransferase. | the membrane lipid phosphatidylcholine (pc) is crucial for stress adaptation and virulence of the plant pathogen agrobacterium tumefaciens. the phospholipid n-methyltransferase pmta catalyzes three successive methylations of phosphatidylethanolamine to yield pc. here, we asked how pmta is recruited to its site of action, the inner leaflet of the membrane. we found that the enzyme attaches to the membrane via electrostatic interactions with anionic lipids, which do not serve as substrate for pmta ... | 2015 | 25403021 |
| protein encoded by oncogene 6b from agrobacterium tumefaciens has a reprogramming potential and histone chaperone-like activity. | crown gall tumors are formed mainly by actions of a group of genes in the t-dna that is transferred from agrobacterium tumefaciens and integrated into the nuclear dna of host plants. these genes encode enzymes for biosynthesis of auxin and cytokinin in plant cells. gene 6b in the t-dna affects tumor morphology and this gene alone is able to induce small tumors on certain plant species. in addition, unorganized calli are induced from leaf disks of tobacco that are incubated on phytohormone-free m ... | 2014 | 25389429 |
| bioactivity of root endophytic freshwater hyphomycetes anguillospora longissima (sacc. & syd.) ingold. | anguillospora longissima, isolated from root as endophytic freshwater hyphomycetes, was evaluated for its bioactivity (antibacterial potential) against five bacterial strains, namely, gram-positive (bacillus subtilis mtcc 121) and gram-negative (agrobacterium tumefaciens mtcc 609, escherichia coli mtcc 40, erwinia chrysanthemum, and xanthomonas pseudomonas). antimicrobial activity was assessed by measuring the zone of inhibition with preliminary and secondary antimicrobial assays. the applied fu ... | 2014 | 25383378 |
| crystal structure of the hiv neutralizing antibody 2g12 in complex with a bacterial oligosaccharide analog of mammalian oligomannose. | human immunodeficiency virus-1 (hiv-1) is a major public health threat that continues to infect millions of people worldwide each year. a prophylactic vaccine remains the most cost-effective way of globally reducing and eliminating the spread of the virus. the hiv envelope spike, which is the target of many vaccine design efforts, is densely mantled with carbohydrate and several potent broadly neutralizing antibodies to hiv-1 recognize carbohydrate on the envelope spike as a major part of their ... | 2014 | 25380763 |
| efficient generation of marker-free transgenic rice plants using an improved transposon-mediated transgene reintegration strategy. | marker-free transgenic plants can be developed through transposon-mediated transgene reintegration, which allows intact transgene insertion with defined boundaries and requires only a few primary transformants. in this study, we improved the selection strategy and validated that the maize (zea mays) activator/dissociation (ds) transposable element can be routinely used to generate marker-free transgenic plants. a ds-based gene of interest was linked to green fluorescent protein in transfer dna ( ... | 2015 | 25371551 |
| quorum sensing in some representative species of halomonadaceae. | cell-to-cell communication, or quorum-sensing (qs), systems are employed by bacteria for promoting collective behaviour within a population. an analysis to detect qs signal molecules in 43 species of the halomonadaceae family revealed that they produced n-acyl homoserine lactones (ahls), which suggests that the qs system is widespread throughout this group of bacteria. thin-layer chromatography (tlc) analysis of crude ahl extracts, using agrobacterium tumefaciens ntl4 (pzlr4) as biosensor strain ... | 2013 | 25371343 |
| cloning of insertion site flanking sequence and construction of transfer dna insert mutant library in stylosanthes colletotrichum. | stylosanthes sp. is the most important forage legume in tropical areas worldwide. stylosanthes anthracnose, which is mainly caused by colletotrichum gloeosporioides, is a globally severe disease in stylo production. little progress has been made in anthracnose molecular pathogenesis research. in this study, agrobacterium tumefaciens-mediated transformation was used to transform stylosanthes colletotrichum strain ch008. the major factors of the genetic transformation system of s. colletotrichum w ... | 2014 | 25361073 |
| multiple enzymatic activities of parb/srx superfamily mediate sexual conflict among conjugative plasmids. | conjugative plasmids are typically locked in intergenomic and sexual conflicts with co-resident rivals, whose translocation they block using fertility inhibition factors (fins). we describe here the first crystal structure of an enigmatic fin osa deployed by the proteobacterial plasmid psa. osa contains a catalytically active version of the parb/sulfiredoxin fold with both atpase and dnase activity, the latter being regulated by an atp-dependent switch. using the agrobacterium tumefaciens virb/d ... | 2014 | 25358815 |
| a novel antibacterial peptide active against peach crown gall (agrobacterium tumefaciens) isolated from cyanide-tolerant actinomycetes g19. | an antimicrobial peptide was extracted from the antagonistic actinomycetes g19. it was designated as g19-f. by using maldi-tof mass spectrometry, the molecular weight of g19-f was determined. the primary structure of the antimicrobial peptide was determined using n-terminal sequencing and mass spectrometry. results showed that the peptide had eleven amino acids, with the sequence d-v-c-d-g-g-d-g-d-e-d, and a calculated molecular mass of 1,096 da. g19-f showed antimicrobial activity against peach ... | 2015 | 25358422 |
| genomic characterization of a temperate phage of the psychrotolerant deep-sea bacterium aurantimonas sp. | a temperate phage (termed amm-1) was identified from the psychrotolerant rhizobiales bacterium aurantimonas sp. c5-1, which was isolated from bathypelagic water (water depth = 1,500 m) in the northwest pacific. the amm-1 genome is 47,800 bp in length and contains 67 coding sequences. although phage amm-1 morphologically belongs to the family myoviridae, its genomic structure, particularly modular genome organization, is similar to that of lambda-type phages of siphoviridae. genetic and phylogene ... | 2015 | 25354565 |
| a comparison of the retention of pathogenic escherichia coli o157 by sprouts, leaves and fruits. | the retention (binding to or association with the plant) of escherichia coli by cut leaves and fruits after vigorous water washing was compared with that by sprouts. retention by fruits and leaves was similar but differed from retention by sprouts in rate, effect of wounding and requirement for poly-β,1-6-n-acetyl-d-glucosamine. escherichia coli was retained by cut ends of lettuce leaves within 5 min while more than 1 h was required for retention by the intact epidermis of leaves and fruits, and ... | 2014 | 25351040 |
| profound impact of hfq on nutrient acquisition, metabolism and motility in the plant pathogen agrobacterium tumefaciens. | as matchmaker between mrna and srna interactions, the rna chaperone hfq plays a key role in riboregulation of many bacteria. often, the global influence of hfq on the transcriptome is reflected by substantially altered proteomes and pleiotropic phenotypes in hfq mutants. using quantitative proteomics and co-immunoprecipitation combined with rna-sequencing (rip-seq) of hfq-bound rnas, we demonstrate the pervasive role of hfq in nutrient acquisition, metabolism and motility of the plant pathogen a ... | 2014 | 25330313 |
| biological activities of the antiviral protein be27 from sugar beet (beta vulgaris l.). | the ribosome inactivating protein be27 displays several biological activities in vitro that could result in a broad action against several types of pathogens. beetin 27 (be27), a ribosome-inactivating protein (rip) from sugar beet (beta vulgaris l.) leaves, is an antiviral protein induced by virus and signaling compounds such as hydrogen peroxide and salicylic acid. its role as a defense protein has been attributed to its rna polynucleotide:adenosine glycosidase activity. here we tested other pu ... | 2015 | 25326773 |
| differentiating indigenous soybean bradyrhizobium and rhizobium spp. of indian soils. | soybean is extensively cultivated worldwide and is the largest source of biologically fixed nitrogen among legumes. it is nodulated by both slow and fast growing rhizobia. indigenous soybean rhizobia in vertisols of central india were assessed for utilization of 35 carbon sources and intrinsic resistance to 19 antibiotics. there was greater utilization of trehalose and raffinose by fast growers (87 and 73 % by fast vs. 35 and 30 % by slow growers); but slow growers had higher ability to utilize ... | 2013 | 25320421 |
| characterization of n-acyl-homoserine lactones (ahls)-deficient clinical isolates of pseudomonas aeruginosa. | pseudomonas aeruginosa is an opportunistic pathogen causing severe respiratory infections. acylated homoserine lactones (ahls) are self-generated diffusible signal molecules that mediate population density dependent gene expression (quorum sensing, qs) in a variety of gram-negative bacteria, and several virulence genes of bacterial pathogens are known to be controlled by qs. hence, fitness mutant of virulent factors is beneficial for natural selection. in this study, strains of p. aeruginosa iso ... | 2014 | 25320416 |
| recombinase polymerase amplification (rpa) of camv-35s promoter and nos terminator for rapid detection of genetically modified crops. | recombinase polymerase amplification (rpa) is a novel isothermal dna amplification and detection technology that enables the amplification of dna within 30 min at a constant temperature of 37-42 °c by simulating in vivo dna recombination. in this study, based on the regulatory sequence of the cauliflower mosaic virus 35s (camv-35s) promoter and the agrobacterium tumefaciens nopaline synthase gene (nos) terminator, which are widely incorporated in genetically modified (gm) crops, we designed two ... | 2014 | 25310647 |
| agrobacterium-mediated genetic transformation of yam (dioscorea rotundata): an important tool for functional study of genes and crop improvement. | although genetic transformation of clonally propagated crops has been widely studied as a tool for crop improvement and as a vital part of the development of functional genomics resources, there has been no report of any existing agrobacterium-mediated transformation of yam (dioscorea spp.) with evidence of stable integration of t-dna. yam is an important crop in the tropics and subtropics providing food security and income to over 300 million people. however, yam production remains constrained ... | 2014 | 25309562 |
| synthesis and quantitative structure activity relationship (qsar) of arylidene (benzimidazol-1-yl)acetohydrazones as potential antibacterial agents. | ethyl (benzimidazol-1-yl)acetate was subjected to hydrazinolysis with hydrazine hydrate to give (benzimidazol-1-yl)acetohydrazide. the latter was reacted with various aromatic aldehydes to give the respective arylidene (1h-benzimidazol-1-yl)acetohydrazones. solutions of the prepared hydrazones were found to contain two geometric isomers. similarly (2-methyl-benzimidazol-1-yl)acetohydrazide was reacted with various aldehydes to give the corresponding hydrazones. the antibacterial activity was eva ... | 2015 | 25304024 |
| the fungal cell-wall integrity mapk cascade is crucial for hyphal network formation and maintenance of restrictive growth of epichloë festucae in symbiosis with lolium perenne. | epichloë festucae is a mutualistic symbiont that systemically colonizes the intercellular spaces of lolium perenne leaves to form a highly structured and interconnected hyphal network. in an agrobacterium tumefaciens t-dna forward genetic screen, we identified a mutant tm1066 that had a severe host interaction phenotype, causing stunting and premature senescence of the host. molecular analysis revealed that the mutation responsible for this phenotype was in the cell-wall integrity (cwi) mitogen- ... | 2015 | 25303335 |
| pepper, sweet (capsicum annuum). | capsicum (pepper) species are economically important crops that are recalcitrant to genetic transformation by agrobacterium (agrobacterium tumefaciens). a number of protocols for pepper transformation have been described but are not routinely applicable. the main bottleneck in pepper transformation is the low frequency of cells that are both susceptible for agrobacterium infection and have the ability to regenerate. here, we describe a protocol for the efficient regeneration of transgenic sweet ... | 2015 | 25300852 |
| soybean [glycine max (l.) merr]. | in this chapter we describe an agrobacterium tumefaciens transformation method of soybean that utilizes mature half seeds and regeneration from the cotyledonary node region. this method results in fertile transformed soybean plants and transgenic seed in approximately 9 months. using mature half seeds as starting material has proven to be a reliable method that does not require additional wounding for infection to occur. we have continued to make improvements in the protocol, resulting in an eff ... | 2015 | 25300848 |
| cowpea [vigna unguiculata (l.) walp]. | agrobacterium tumefaciens-mediated transformation is an efficient method for incorporating genes and recovering stable transgenic plants in cowpea because this method offers several advantages such as the defined integration of transgenes, potentially low copy number, and preferential integration into transcriptional active regions of the chromosome. cotyledonary node explants of cowpea present an attractive target for t-dna delivery followed by regeneration of shoots via axillary proliferation ... | 2015 | 25300846 |
| wheat (triticum aestivum l.) transformation using immature embryos. | wheat may now be transformed very efficiently by agrobacterium tumefaciens. under the protocol hereby described, immature embryos of healthy plants of wheat cultivar fielder grown in a well-conditioned greenhouse were pretreated with centrifuging and cocultivated with a. tumefaciens. transgenic wheat plants were obtained routinely from between 40 and 90 % of the immature embryos, thus infected in our tests. all regenerants were normal in morphology and fully fertile. about half of the transforme ... | 2015 | 25300841 |
| rice, indica (oryza sativa l.). | indica varieties, which are generally recalcitrant to tissue culture and transformation, occupy 80 % of rice cultivation area in the world. therefore, transformation method for indica rice must be improved greatly so that global rice production would take full advantage of cutting-edge biotechnology. an efficient protocol for indica transformation mediated by agrobacterium tumefaciens is hereby described. immature embryos collected from plants in a greenhouse are cocultivated with a. tumefaciens ... | 2015 | 25300838 |
| maize (zea mays l.). | agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. in this chapter, we describe a detailed protocol for genetic transformation of the maize genotype hi ii. our starting plant material is immature embryos cocultivated with an agrobacterium strain carrying a standard binary vector. in addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plants in the gree ... | 2015 | 25300834 |
| barley (hordeum vulgare l.) transformation using immature embryos. | barley is a major crop species, and also has become a genetic model for the small grain temperate cereals. a draft barley genome sequence has recently been completed, opening many opportunities for candidate gene isolation and functionality testing. thanks to the development of customizable endonucleases, also site-directed genome modification recently became feasible for higher plants, which marks the beginning of a new era of genetic engineering. the development of improved binary vectors and ... | 2015 | 25300832 |
| setaria viridis. | interest in plant model systems for genetic, biological, and functional genomics studies stems from advantages they provide in terms of fast generation time, small stature, and simple growth requirements. a model species would be especially advantageous for the studies of c4 photosynthetic grasses, which currently present practical challenges. these include long seed-to-seed generation times, and because of their large size at maturity they require large growing areas. one potential model of int ... | 2015 | 25300831 |
| brachypodium distachyon. | the small grass brachypodium distachyon has attributes that make it an excellent model for the development and improvement of cereal crops and bioenergy feedstocks. to realize the potential of this system, many tools have been developed (e.g., the complete genome sequence, a large collection of natural accessions, a high density genetic map, bac libraries, est sequences, microarrays, etc.). in this chapter, we describe a high-efficiency transformation system, an essential tool for a modern model ... | 2015 | 25300828 |
| agrobacterium uses a unique ligand-binding mode for trapping opines and acquiring a competitive advantage in the niche construction on plant host. | by modifying the nuclear genome of its host, the plant pathogen agrobacterium tumefaciens induces the development of plant tumours in which it proliferates. the transformed plant tissues accumulate uncommon low molecular weight compounds called opines that are growth substrates for a. tumefaciens. in the pathogen-induced niche (the plant tumour), a selective advantage conferred by opine assimilation has been hypothesized, but not experimentally demonstrated. here, using genetics and structural b ... | 2014 | 25299655 |
| tccys4, a cystatin from cocoa, reduces necrosis triggered by mpnep2 in tobacco plants. | in brazil, most cocoa bean production occurs in southern bahia. witches' broom disease arrived in this area in 1989 and has since caused heavy losses in production. the disease is caused by the basidiomycete fungus moniliophthora perniciosa, a hemibiotrophic fungus that produces the necrosis and ethylene-inducting protein (mpnep2) during infection; this protein can activate cysteine proteases and induce programmed cell death. cysteine proteases can be modulated by cystatin. in this study, we ove ... | 2014 | 25299077 |
| genetic transformation of metroxylon sagu (rottb.) cultures via agrobacterium-mediated and particle bombardment. | sago palm (metroxylon sagu) is a perennial plant native to southeast asia and exploited mainly for the starch content in its trunk. genetic improvement of sago palm is extremely slow when compared to other annual starch crops. urgent attention is needed to improve the sago palm planting material and can be achieved through nonconventional methods. we have previously developed a tissue culture method for sago palm, which is used to provide the planting materials and to develop a genetic transform ... | 2014 | 25295258 |
| microsymbionts of phaseolus vulgaris in acid and alkaline soils of mexico. | in order to investigate bean-nodulating rhizobia in different types of soil, 41 nodule isolates from acid and alkaline soils in mexico were characterized. based upon the phylogenetic studies of 16s rrna, atpd, glnii, reca, rpob, gyrb, nifh and nodc genes, the isolates originating from acid soils were identified as the phaseoli symbiovar of the rhizobium leguminosarum-like group and rhizobium grahamii, whereas the isolates from alkaline soils were defined as ensifer americanum sv. mediterranense ... | 2014 | 25294010 |
| temperature effects on bacterial phytochrome. | bacteriophytochromes (bphps) are light-sensing regulatory proteins encoded in photosynthetic and non-photosynthetic bacteria. this protein class incorporate bilin as their chromophore, with majority of them bearing a light- regulated his kinase or his kinase related module in the c-terminal. we studied the his kinase actives in the temperature range of 5°c to 40°c on two bphps, agp1 from agrobacterium tumefaciens and cph1 from cyanobacterium synechocystis pcc 6803. as reported, the phosphorylati ... | 2014 | 25289638 |
| agrobacterium radiobacter (beijerinck and van delden 1902) conn 1942 has priority over agrobacterium tumefaciens (smith and townsend 1907) conn 1942 when the two are treated as members of the same species based on the principle of priority and rule 23a, note 1 as applied to the corresponding specific epithets. opinion 94. judicial commission of the international committee on systematics of prokaryotes. | the judicial commission affirms that, according to the rules of the international code of nomenclature of bacteria (including changes made to the wording), the combination agrobacterium radiobacter (beijerinck and van delden 1902) conn 1942 has priority over the combination agrobacterium tumefaciens (smith and townsend 1907) conn 1942 when the two are treated as members of the same species based on the principle of priority as applied to the corresponding specific epithets. the type species of t ... | 2014 | 25288664 |
| characterization of the promoter of grapevine vein clearing virus. | grapevine vein clearing virus (gvcv) is a recently discovered dna virus in grapevine that is closely associated with the grapevine vein clearing syndrome observed in vineyards in missouri and surrounding states. the genome sequence of gvcv indicates that it belongs to the genus badnavirus in the family caulimoviridae. to identify the gvcv promoter, we cloned portions of the gvcv large intergenic region in front of a gfp gene present in an agrobacterium tumefaciens binary vector. gfp expression w ... | 2015 | 25281563 |
| modifying fatty acid profiles through a new cytokinin-based plastid transformation system. | the widespread use of herbicides and antibiotics for selection of transgenic plants has not been very successful with regard to commercialization and public acceptance. hence, alternative selection systems are required. in this study, we describe the use of ipt, the bacterial gene encoding the enzyme isopentenyl transferase from agrobacterium tumefaciens, as a positive selectable marker for plastid transformation. a comparison between the traditional spectinomycin-based aada selection system and ... | 2014 | 25280363 |
| new developments of rnai in paracoccidioides brasiliensis: prospects for high-throughput, genome-wide, functional genomics. | the fungal genome initiative of the broad institute, in partnership with the paracoccidioides research community, has recently sequenced the genome of representative isolates of this human-pathogen dimorphic fungus: pb18 (s1), pb03 (ps2) and pb01. the accomplishment of future high-throughput, genome-wide, functional genomics will rely upon appropriate molecular tools and straightforward techniques to streamline the generation of stable loss-of-function phenotypes. in the past decades, rnai has e ... | 2014 | 25275433 |
| ras gtpase activating protein coira1 is involved in infection-related morphogenesis by regulating camp and mapk signaling pathways through coras2 in colletotrichum orbiculare. | colletotrichum orbiculare is the causative agent of anthracnose disease on cucurbitaceous plants. several signaling pathways, including camp-pka and mitogen-activating protein kinase (mapk) pathways are involved in the infection-related morphogenesis and pathogenicity of c. orbiculare. however, upstream regulators of these pathways for this species remain unidentified. in this study, coira1, encoding ras gtpase activating protein, was identified by screening the agrobacterium tumefaciens-mediate ... | 2014 | 25275394 |
| development of a transformation system for aspergillus sojae based on the agrobacterium tumefaciens-mediated approach. | aspergillus sojae has been an important filamentous fungus in biotechnology due to its use in diverse fermentative processes for the production of various food products. furthermore, this fungus is a common expression system for the production of enzymes and other metabolites. the availability of molecular genetic tools to explore its biology is thus of big interest. in this study, an agrobacterium tumefaciens-mediated transformation (atmt) system for a. sojae was developed and its applicability ... | 2014 | 25253558 |
| stress induced acquisition of somatic embryogenesis in common bean phaseolus vulgaris l. | common bean phaseolus vulgaris l. has been shown to be a recalcitrant plant to induce somatic embryogenesis (se) under in vitro conditions. we used an alternative strategy to induce se in common bean based upon the use of a cytokinin (bap) coupled with osmotic stress adaptation instead of se response that is induced by auxins. explants derived from zygotic embryos of common bean were subjected to osmotic stress (sucrose 12 % w/v, 0.5 m) in the presence of bap 10 mg/l and adenine free base 40 mg/ ... | 2015 | 25252886 |
| the impact of pseudomonas syringae type iii effectors on transient protein expression in tobacco. | the production of recombinant proteins in plants is often achieved by transient expression, e.g. following the injection or vacuum infiltration of agrobacterium tumefaciens into tobacco leaves. we investigated the associated plant defence responses, revealing that callose deposition is triggered by t-dna transfer and that subsets of secondary metabolites accumulate in response to mechanical wounding or the presence of bacteria. we also tested the ability of five co-expressed type iii effector pr ... | 2015 | 25243954 |
| towards understanding the molecular recognition process in prokaryotic zinc-finger domain. | eukaryotic cys2his2 zinc finger domain is one of the most common and important structural motifs involved in protein-dna interaction. the recognition motif is characterized by the tetrahedral coordination of a zinc ion by conserved cysteine and histidine residues. we have characterized the prokaryotic cys2his2 zinc finger motif, included in the dna binding region (ros87) of ros protein from agrobacterium tumefaciens, demonstrating that, although possessing a similar zinc coordination sphere, thi ... | 2015 | 25240418 |
| conversion of bac clones into binary bac (bibac) vectors and their delivery into basidiomycete fungal cells using agrobacterium tumefaciens. | the genetic transformation of certain organisms, required for gene function analysis or complementation, is often not very efficient, especially when dealing with large gene constructs or genomic fragments. we have adapted the natural dna transfer mechanism from the soil pathogenic bacterium agrobacterium tumefaciens, to deliver intact large dna constructs to basidiomycete fungi of the genus ustilago where they stably integrated into their genome. to this end, bacterial artificial chromosome (ba ... | 2015 | 25239747 |
| bioactive spirobisnaphthalenes from the endophytic fungus berkleasmium sp. | nine new spirobisnaphthalenes, palmarumycins b1-b9 (1-9), along with 13 known compounds (10-22), were isolated from cultures of the fungus berkleasmium sp., an endophyte isolated from the medicinal plant dioscorea zingiberensis c. h. wright. the structures of the new compounds were elucidated by analysis of the 1d and 2d nmr and hresims spectra and by comparison with known compounds. compounds 7-9 contain an uncommon 2,3-dihydro-1h-inden-1-one unit. all isolated compounds were evaluated for thei ... | 2014 | 25237727 |
| control of zinc homeostasis in agrobacterium tumefaciens via zur and the zinc uptake genes znuabc and zint. | the agrobacterium tumefaciens zinc uptake regulator (zur) was shown to negatively regulate the zinc uptake genes znuabc, encoding a zinc transport system belonging to the atp-binding cassette (abc) transporter family, and zint, which encodes a periplasmic zinc-binding protein. the expression of znuabc and zint was inducible when cells were grown in medium containing a metal chelator (edta), and this induction was shown to be specific for zinc depletion. the expression of znuabc was reduced in re ... | 2014 | 25227896 |
| delineation of polar localization domains of agrobacterium tumefaciens type iv secretion apparatus proteins virb4 and virb11. | agrobacterium tumefaciens transfers dna and proteins to a plant cell through a type iv secretion apparatus assembled by the virb proteins. all virb proteins localized to a cell pole, although these conclusions are in dispute. to study subcellular location of the virb proteins and to identify determinants of their subcellular location, we tagged two proteins, virb4 and virb11, with the visual marker green fluorescent protein (gfp) and studied localization of the fusion proteins by epifluorescence ... | 2014 | 25220247 |
| over-expression of osptr6 in rice increased plant growth at different nitrogen supplies but decreased nitrogen use efficiency at high ammonium supply. | nitrogen (n) plays a critical role in plant growth and productivity and ptr/nrt1 transporters are critical for rice growth. in this study, osptr6, a ptr/nrt1 transporter, was over-expressed in the nipponbare rice cultivar by agrobacterium tumefaciens transformation using the ubiquitin (ubi) promoter. three single-copy t2 generation transgenic lines, named oe1, oe5 and oe6, were produced and subjected to hydroponic growth experiments in different nitrogen treatments. the results showed the plant ... | 2014 | 25219300 |
| beyond my wildest expectations. | with support from my parents, i fulfilled their and my expectations of graduating from college and becoming a scientist. my scientific career has focused on two organisms, bacillus subtilis and agrobacterium tumefaciens, and two experimental systems, aromatic amino acid synthesis and dna transfer in bacteria and plants. studies on b. subtilis emphasized the genetics and biochemistry of aromatic amino acid synthesis and the characterization of competence in dna transformation. i carried out both ... | 2014 | 25208299 |
| [hair roots induction and culture of withania somnifera and its withanolide a synthesis]. | withanolide a is a biologically active secondary metabolite occuring in roots and leaves of withania somnifera. in the present study, adventitious roots from leaf explants of w. somnifera were induced for the production of withanolide-a by agrobacterium tumefaciens strain c58c1 to obtain hair roots. hair roots induction rate reached 30%. the withanolide a was determined by hplc in different hair roots lines and different parts of w. somnifera. the average content of withanolide a in all hair roo ... | 2014 | 25204166 |
| native mitral valve endocarditis due to rhizobium radiobacter - first case report. | rhizobium radiobacter (rr) has rarely been associated with human infection, mainly sepsis or bacteremia, and an unique case of prosthetic aortic endocarditis has been reported. we present a case of native mitral valve endocarditis to rr, to our knowledge the first clinical report of such infection. | 2014 | 25202754 |
| genomic analysis of agrobacterium radiobacter dsm 30147(t) and emended description of a. radiobacter (beijerinck and van delden 1902) conn 1942 (approved lists 1980) emend. sawada et al. 1993. | agrobacterium radiobacter is the only known non-phytopathogenic species in agrobacterium genus. in this study, the whole-genome sequence of a. radiobacter type strain dsm 30147(t) was described and compared to the other available agrobacterium genomes. this bacterium has a genome size of 7,122,065 bp distributed in 612 contigs, including 6,834 protein-coding genes and 41 rna genes. it harbors a circular chromosome and a linear chromosome but not a tumor-inducing (ti) plasmid. to the best of our ... | 2014 | 25197445 |