Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| current and past strategies for bacterial culture in clinical microbiology. | a pure bacterial culture remains essential for the study of its virulence, its antibiotic susceptibility, and its genome sequence in order to facilitate the understanding and treatment of caused diseases. the first culture conditions empirically varied incubation time, nutrients, atmosphere, and temperature; culture was then gradually abandoned in favor of molecular methods. the rebirth of culture in clinical microbiology was prompted by microbiologists specializing in intracellular bacteria. th ... | 2015 | 25567228 |
| interleukin-6 c.-174g>c polymorphism and periodontitis in a brazilian population. | aim. periodontitis is an inflammatory disease that affects the teeth supporting structures, triggered by periodontal pathogens, and is influenced by environmental and genetic factors. genes encoding molecules related to the immune response, such as cytokine, are the main candidates for polymorphisms analysis and may be possibly associated with this pathology. a g/c promoter polymorphism on the il6 gene has been shown to affect basal il-6 levels. the aim of this study was to investigate the assoc ... | 2014 | 25548674 |
| porphyromonas gingivalis and related bacteria: from colonial pigmentation to the type ix secretion system and gliding motility. | porphyromonas gingivalis is a gram-negative, non-motile, anaerobic bacterium implicated as a major pathogen in periodontal disease. p. gingivalis grows as black-pigmented colonies on blood agar, and many bacteriologists have shown interest in this property. studies of colonial pigmentation have revealed a number of important findings, including an association with the highly active extracellular and surface proteinases called gingipains that are found in p. gingivalis. the por secretion system, ... | 2014 | 25546073 |
| porphyromonas gingivalis and related bacteria: from colonial pigmentation to the type ix secretion system and gliding motility. | porphyromonas gingivalis is a gram-negative, non-motile, anaerobic bacterium implicated as a major pathogen in periodontal disease. p. gingivalis grows as black-pigmented colonies on blood agar, and many bacteriologists have shown interest in this property. studies of colonial pigmentation have revealed a number of important findings, including an association with the highly active extracellular and surface proteinases called gingipains that are found in p. gingivalis. the por secretion system, ... | 2014 | 25546073 |
| complete genome sequence for treponema sp. omz 838 (atcc 700772, dsm 16789), isolated from a necrotizing ulcerative gingivitis lesion. | the oral treponeme bacterium treponema sp. omz 838 was originally isolated from a human necrotizing ulcerative gingivitis (nug) lesion. its taxonomic status remains uncertain. the complete genome sequence length was determined to be 2,708,067 bp, with a g+c content of 44.58%, and 2,236 predicted coding dna sequences (cds). | 2014 | 25540346 |
| microbial leakage at the implant-abutment connection due to implant insertion maneuvers: cross-sectional study 5 years postloading in healthy patients. | the aim of this study was to test if stress on the prosthetic connection during insertion maneuvers can induce micro-warping at the implant connection. from september 2011 to july 2013, patients with implants loaded for at least 5 years that were placed with 2 different insertion implant mounters-mp (conventional) and me (mountless)-were selected from all of those who had received dental implant therapy in the past and were attending routine check-up or spontaneous visits during the study period ... | 2015 | 25536224 |
| periodontitis: from microbial immune subversion to systemic inflammation. | periodontitis is a dysbiotic inflammatory disease with an adverse impact on systemic health. recent studies have provided insights into the emergence and persistence of dysbiotic oral microbial communities that can mediate inflammatory pathology at local as well as distant sites. this review discusses the mechanisms of microbial immune subversion that tip the balance from homeostasis to disease in oral or extra-oral sites. | 2015 | 25534621 |
| distribution of 8 periodontal microorganisms in family members of chinese patients with aggressive periodontitis. | to date, no information on the distribution of periodontal microorganisms among family members of chinese patients with aggressive peridontitis (agp) is available. the aim of the present study was to investigate the probability of transmission of eight periodontal microorganisms between patients with aggressive periodontitis and their family members. | 2015 | 25526624 |
| fimbriae-mediated outer membrane vesicle production and invasion of porphyromonas gingivalis. | porphyromonas gingivalis is a keystone periopathogen that plays an essential role in the progress of periodontitis. like other gram-negative bacteria, the ability of p. gingivalis to produce outer membrane vesicles is a strategy used to interact with, and survive within its biological niches. here we compared the protein components associated with vesicles derived from a fimbriated strain (33277) and an afimbriated strain (w83) of p. gingivalis using proteomic analyses. some well-known virulence ... | 2014 | 25524808 |
| fimbriae-mediated outer membrane vesicle production and invasion of porphyromonas gingivalis. | porphyromonas gingivalis is a keystone periopathogen that plays an essential role in the progress of periodontitis. like other gram-negative bacteria, the ability of p. gingivalis to produce outer membrane vesicles is a strategy used to interact with, and survive within its biological niches. here we compared the protein components associated with vesicles derived from a fimbriated strain (33277) and an afimbriated strain (w83) of p. gingivalis using proteomic analyses. some well-known virulence ... | 2014 | 25524808 |
| microbiological effects of periodontal therapy plus azithromycin in patients with diabetes: results from a randomized clinical trial. | current evidence suggests that periodontal infection may aggravate diabetes control. the aim of this study was to determine the changes in the frequency with which porphyromonas gingivalis, tannerella forsythia, treponema denticola and aggregatibacter actinomycetemcomitans were detected in patients with diabetes with the use of non-surgical therapy plus azithromycin in a randomized clinical trial. one hundred and five (105) patients with diabetes and chronic periodontitis were randomly assigned ... | 2014 | 25523961 |
| serum igg antibody levels to periodontal microbiota are associated with incident alzheimer disease. | periodontitis and alzheimer disease (ad) are associated with systemic inflammation. this research studied serum igg to periodontal microbiota as possible predictors of incident ad. | 2014 | 25522313 |
| prevalence and quantitative analysis of epstein-barr virus dna and porphyromonas gingivalis associated with japanese chronic periodontitis patients. | a number of studies have recently suggested epstein-barr virus (ebv) involvement in the pathogenesis of periodontitis. in this study, we investigated the association between major periodontopathic bacteria porphyromonas gingivalis (p. gingivalis) and ebv in japanese chronic periodontitis (cp) patients. | 2014 | 25515271 |
| prevalence and quantitative analysis of epstein-barr virus dna and porphyromonas gingivalis associated with japanese chronic periodontitis patients. | a number of studies have recently suggested epstein-barr virus (ebv) involvement in the pathogenesis of periodontitis. in this study, we investigated the association between major periodontopathic bacteria porphyromonas gingivalis (p. gingivalis) and ebv in japanese chronic periodontitis (cp) patients. | 2014 | 25515271 |
| formylglycine, a post-translationally generated residue with unique catalytic capabilities and biotechnology applications. | formylglycine (fgly) is a catalytically essential residue found almost exclusively in the active sites of type i sulfatases. formed by post-translational oxidation of cysteine or serine side chains, this aldehyde-functionalized residue participates in a unique and highly efficient catalytic mechanism for sulfate ester hydrolysis. the enzymes that produce fgly, formylglycine-generating enzyme (fge) and anaerobic sulfatase-maturating enzyme (ansme), are as unique and specialized as fgly itself. fg ... | 2015 | 25514000 |
| antimicrobial effect of ozonated water, sodium hypochlorite and chlorhexidine gluconate in primary molar root canals. | the aim was to determine the antimicrobial effect of ozonated water, ozonated water with ultrasonication, sodium hypochloride and chlorhexidine (chx) in human primary root canals contaminated by enterococcus faecalis (e. faecalis). | 2014 | 25512726 |
| subgingival microbial profile and production of proinflammatory cytokines in chronic periodontitis. | this review examines literature data concerning the bacterial findings in chronic periodontitis depending on pocket depth, and presents the latest published information on the presence of proinflammatory factors in periodontal environment. it has been found that chronic periodontitis affects as much as 80% of the middle-aged population; by comparison, the prevalence of aggressive periodontitis reaches up to 1-1.5%. it is accepted that this social disease is multifactorial in etiology, but the ev ... | 2014 | 25507669 |
| investigate the correlation between clinical sign and symptoms and the presence of p. gingivalis, t. denticola, and t. forsythia individually or as a "red complex" by a multiplex pcr method. | the aim of this study was to investigate the correlation between endodontic clinical signs and symptoms and the presence of porphyromonas gingivalis, treponema denticola, and tannerella forsythia or their association by multiplex polymerase chain reaction assay. | 2014 | 25506144 |
| polymicrobial synergy and dysbiosis in inflammatory disease. | uncontrolled inflammation of the periodontal area may arise when complex microbial communities transition from a commensal to a pathogenic entity. communication among constituent species leads to polymicrobial synergy between metabolically compatible organisms that acquire functional specialization within the developing community. keystone pathogens, even at low abundance, elevate community virulence, and the resulting dysbiotic community targets specific aspects of host immunity to further disa ... | 2014 | 25498392 |
| polymicrobial synergy and dysbiosis in inflammatory disease. | uncontrolled inflammation of the periodontal area may arise when complex microbial communities transition from a commensal to a pathogenic entity. communication among constituent species leads to polymicrobial synergy between metabolically compatible organisms that acquire functional specialization within the developing community. keystone pathogens, even at low abundance, elevate community virulence, and the resulting dysbiotic community targets specific aspects of host immunity to further disa ... | 2014 | 25498392 |
| identification of dipeptidyl-peptidase (dpp)5 and dpp7 in porphyromonas endodontalis, distinct from those in porphyromonas gingivalis. | dipeptidyl peptidases (dpps) that liberate dipeptides from the n-terminal end of oligopeptides are crucial for the growth of porphyromonas species, anaerobic asaccharolytic gram negative rods that utilize amino acids as energy sources. porphyromonas endodontalis is a causative agent of periapical lesions with acute symptoms and asp/glu-specific dpp11 has been solely characterized in this organism. in this study, we identified and characterized two p. endodontalis dpps, dpp5 and dpp7. cell-associ ... | 2014 | 25494328 |
| periodontal disease associates with higher brain amyloid load in normal elderly. | the accumulation of amyloid-β (aβ) plaques is a central feature of alzheimer's disease (ad). first reported in animal models, it remains uncertain if peripheral inflammatory and/or infectious conditions in humans can promote aβ brain accumulation. periodontal disease, a common chronic infection, has been previously reported to be associated with ad. thirty-eight cognitively normal, healthy, and community-residing elderly (mean age, 61 and 68% female) were examined in an alzheimer's disease resea ... | 2014 | 25491073 |
| periodontal disease associates with higher brain amyloid load in normal elderly. | the accumulation of amyloid-β (aβ) plaques is a central feature of alzheimer's disease (ad). first reported in animal models, it remains uncertain if peripheral inflammatory and/or infectious conditions in humans can promote aβ brain accumulation. periodontal disease, a common chronic infection, has been previously reported to be associated with ad. thirty-eight cognitively normal, healthy, and community-residing elderly (mean age, 61 and 68% female) were examined in an alzheimer's disease resea ... | 2014 | 25491073 |
| subgingival microbial profiles of sudanese patients with aggressive periodontitis. | aggressive periodontitis (agp) is prevalent and shows a rapid course in african individuals. although a strong focus has been placed on aggregatibacter actinomycetemcomitans, new methods support the existence of a complex subgingival microflora in agp. the purpose of the present study was to map the subgingival microbiota as well as explore the presence of a. actinomycetemcomitans and the jp2 clone in a group of sudanese individuals with agp, using different analytical methods. | 2014 | 25487558 |
| subgingival microbial profiles of sudanese patients with aggressive periodontitis. | aggressive periodontitis (agp) is prevalent and shows a rapid course in african individuals. although a strong focus has been placed on aggregatibacter actinomycetemcomitans, new methods support the existence of a complex subgingival microflora in agp. the purpose of the present study was to map the subgingival microbiota as well as explore the presence of a. actinomycetemcomitans and the jp2 clone in a group of sudanese individuals with agp, using different analytical methods. | 2014 | 25487558 |
| the novel species streptococcus tigurinus and its association with oral infection. | streptococcus tigurinus is a novel species of viridans streptococci, shown to cause severe invasive infections such as infective endocarditis, spondylodiscitis and meningitis. s. tigurinus belongs to the streptococcus mitis group and is most closely related to streptococcus mitis, streptococcus oralis, streptococcus pneumoniae, streptococcus pseudopneumoniae and streptococcus infantis. the presence of s. tigurinus in the human oral cavity has been documented, including in patients with periodont ... | 2014 | 25483862 |
| the novel species streptococcus tigurinus and its association with oral infection. | streptococcus tigurinus is a novel species of viridans streptococci, shown to cause severe invasive infections such as infective endocarditis, spondylodiscitis and meningitis. s. tigurinus belongs to the streptococcus mitis group and is most closely related to streptococcus mitis, streptococcus oralis, streptococcus pneumoniae, streptococcus pseudopneumoniae and streptococcus infantis. the presence of s. tigurinus in the human oral cavity has been documented, including in patients with periodont ... | 2014 | 25483862 |
| bacteriophage resistance mechanisms in the fish pathogen flavobacterium psychrophilum: linking genomic mutations to changes in bacterial virulence factors. | flavobacterium psychrophilum is an important fish pathogen in salmonid aquaculture worldwide. due to increased antibiotic resistance, pathogen control using bacteriophages has been explored as a possible alternative treatment. however, the effective use of bacteriophages in pathogen control requires overcoming the selection for phage resistance in the bacterial populations. here, we analyzed resistance mechanisms in f. psychrophilum after phage exposure using whole-genome sequencing of the ances ... | 2015 | 25480749 |
| s-nitrosoglutathione accelerates recovery from 5-fluorouracil-induced oral mucositis. | mucositis induced by anti-neoplastic drugs is an important, dose-limiting and costly side-effect of cancer therapy. | 2014 | 25478918 |
| rho and rnase play a central role in fmn riboswitch regulation in corynebacterium glutamicum. | riboswitches are rna elements that regulate gene expression in response to their ligand. although these regulations are thought to be performed without any aid of other factors, recent studies suggested the participation of protein factors such as transcriptional termination factor rho and rnase in some riboswitch regulations. however, to what extent these protein factors contribute to the regulation was unclear. here, we studied the regulatory mechanism of the flavin mononucleotide (fmn) ribosw ... | 2014 | 25477389 |
| rho and rnase play a central role in fmn riboswitch regulation in corynebacterium glutamicum. | riboswitches are rna elements that regulate gene expression in response to their ligand. although these regulations are thought to be performed without any aid of other factors, recent studies suggested the participation of protein factors such as transcriptional termination factor rho and rnase in some riboswitch regulations. however, to what extent these protein factors contribute to the regulation was unclear. here, we studied the regulatory mechanism of the flavin mononucleotide (fmn) ribosw ... | 2014 | 25477389 |
| high-resolution taxonomic profiling of the subgingival microbiome for biomarker discovery and periodontitis diagnosis. | the oral microbiome plays a key role for caries, periodontitis, and systemic diseases. a method for rapid, high-resolution, robust taxonomic profiling of subgingival bacterial communities for early detection of periodontitis biomarkers would therefore be a useful tool for individualized medicine. here, we used illumina sequencing of the v1-v2 and v5-v6 hypervariable regions of the 16s rrna gene. a sample stratification pipeline was developed in a pilot study of 19 individuals, 9 of whom had been ... | 2015 | 25452281 |
| electrophoresis of periodontal pathogens in poly(ethyleneoxide) solutions with uncoated capillary. | periodontitis is a prevalent inflammatory disease caused by different species of anaerobic bacteria such as porphyromonas gingivalis (p.g), treponema denticola (t.d), and tannerella forsythia (t.f). we compared the separation result of dna ladders in hydroxyethyl cellulose, poly(ethyleneoxide) (peo), and polyethylene glycol and analyzed the effect of polymer concentration, electric field, and temperature of the background electrolyte on the separation performance. results demonstrated that there ... | 2015 | 25447490 |
| the oral microbiome and the immunobiology of periodontal disease and caries. | the composition of the oral microbiome differs from one intraoral site to another, reflecting in part the host response and immune capacity at each site. by focusing on two major oral infections, periodontal disease and caries, new principles of disease emerge. periodontal disease affects the soft tissues and bone that support the teeth. caries is a unique infection of the dental hard tissues. the initiation of both diseases is marked by an increase in the complexity of the microbiome. in period ... | 2014 | 25447398 |
| a yada-like autotransporter, hag1 in veillonella atypica is a multivalent hemagglutinin involved in adherence to oral streptococci, porphyromonas gingivalis, and human oral buccal cells. | dental biofilm development is a sequential process, and adherence between microbes and the salivary pellicle (adhesion) as well as among different microbes (co-adhesion or coaggregation) plays a critical role in building a biofilm community. the veillonella species are among the most predominant species in the oral cavity and coaggregate with many initial, early, middle, and late colonizers. similar to oral fusobacteria, they are also considered bridging species in biofilm development. however, ... | 2015 | 25440509 |
| the gastrointestinal microbiome and musculoskeletal diseases: a beneficial role for probiotics and prebiotics. | natural medicines are an attractive option for patients diagnosed with common and debilitating musculoskeletal diseases such as osteoarthritis (oa) or rheumatoid arthritis (ra). the high rate of self-medication with natural products is due to (1) lack of an available cure and (2) serious adverse events associated with chronic use of pharmaceutical medications in particular non-steroidal anti-inflammatory drugs (nsaids) and high dose paracetamol. pharmaceuticals to treat pain may disrupt gastroin ... | 2013 | 25437335 |
| from in vitro to in vivo models of bacterial biofilm-related infections. | the influence of microorganisms growing as sessile communities in a large number of human infections has been extensively studied and recognized for 30-40 years, therefore warranting intense scientific and medical research. nonetheless, mimicking the biofilm-life style of bacteria and biofilm-related infections has been an arduous task. models used to study biofilms range from simple in vitro to complex in vivo models of tissues or device-related infections. these different models have progressi ... | 2013 | 25437038 |
| epithelial antimicrobial peptides: guardian of the oral cavity. | gingival epithelium provides first line of defence from the microorganisms present in dental plaque. it not only provides a mechanical barrier but also has an active immune function too. gingival epithelial cells participate in innate immunity by producing a range of antimicrobial peptides to protect the host against oral pathogens. these epithelial antimicrobial peptides (eaps) include the β-defensin family, cathelicidin (ll-37), calprotectin, and adrenomedullin. while some are constitutively e ... | 2014 | 25435884 |
| subgingival microbial profile and production of proinflammatory cytokines in chronic periodontitis. | this review examines literature data concerning the bacterial findings in chronic periodontitis depending on pocket depth, and presents the latest published information on the presence of proinflammatory factors in periodontal environment. it has been found that chronic periodontitis affects as much as 80% of the middle-aged population; by comparison, the prevalence of aggressive periodontitis reaches up to 1-1.5%. it is accepted that this social disease is multifactorial in etiology, but the ev ... | 2014 | 25434071 |
| detection of periodontal pathogens in the patients with aortic aneurysm. | the occurrence and development of aortic aneurysm (aa) are associated with infection. some researchers have detected the dna of periodontal pathogens in aa samples in certain populations. however, it has not been done in chinese population. the objective of this study was to evaluate the prevalence of periodontal pathogens in oral tissue samples and aneurysm samples of aa patients. | 2014 | 25430459 |
| detection rates of presumptive periodontal pathogens in subgingival plaque samples of untreated periodontitis using either four or six pooled samples. | a comparison of the detection frequency and number of periodontal pathogens in patients with aggressive or generalized, severe chronic periodontitis using a gene-probe analysis. | 2010 | 25427269 |
| orthodontic bracket designs and their impact on microbial profile and periodontal disease: a clinical trial. | the aim of the present study was to compare the undisturbed plaque formation on teeth bonded with preadjusted (captain ortho, libral traders, mumbai, india) and begg brackets (captain ortho, libral traders, mumbai, india) with nonbonded control sites via a de novo plaque growth over a period of 7 days. | 2014 | 25426456 |
| strand invasion based amplification (siba®): a novel isothermal dna amplification technology demonstrating high specificity and sensitivity for a single molecule of target analyte. | isothermal nucleic acid amplification technologies offer significant advantages over polymerase chain reaction (pcr) in that they do not require thermal cycling or sophisticated laboratory equipment. however, non-target-dependent amplification has limited the sensitivity of isothermal technologies and complex probes are usually required to distinguish between non-specific and target-dependent amplification. here, we report a novel isothermal nucleic acid amplification technology, strand invasion ... | 2014 | 25419812 |
| shifts in campylobacter species abundance may reflect general microbial community shifts in periodontitis progression. | oral campylobacter species have been found to be associated with periodontitis progression. while the etiological significance of campylobacter rectus is quite established, the association of c. gracilis, c. concisus, and c. curvus with health or disease remains contradictory. | 2014 | 25412608 |
| oral bacterial dna findings in pericardial fluid. | we recently reported that large amounts of oral bacterial dna can be found in thrombus aspirates of myocardial infarction patients. some case reports describe bacterial findings in pericardial fluid, mostly done with conventional culturing and a few with pcr; in purulent pericarditis, nevertheless, bacterial pcr has not been used as a diagnostic method before. | 2014 | 25412607 |
| characterization of treponema denticola mutants defective in the major antigenic proteins, msp and tmpc. | treponema denticola, a gram-negative and anaerobic spirochete, is associated with advancing severity of chronic periodontitis. in this study, we confirmed that two major antigenic proteins were msp and tmpc, and examined their physiological and pathological roles using gene-deletion mutants. msp formed a large complex that localized to the outer membrane, while tmpc existed as a monomer and largely localized to the inner membrane. however, tmpc was also detected in the outer membrane fraction, b ... | 2014 | 25401769 |
| periodontal and peri-implant microbiota in patients with healthy and inflamed periodontal and peri-implant tissues. | to compare the prevalence and levels of six bacterial pathogens within the subgingival/submucosal microbiota at teeth versus implants with various clinical conditions. | 2016 | 25399962 |
| pre-steady-state kinetic and structural analysis of interaction of methionine γ-lyase from citrobacter freundii with inhibitors. | methionine γ-lyase (mgl) catalyzes the γ-elimination of l-methionine and its derivatives as well as the β-elimination of l-cysteine and its analogs. these reactions yield α-keto acids and thiols. the mechanism of chemical conversion of amino acids includes numerous reaction intermediates. the detailed analysis of mgl interaction with glycine, l-alanine, l-norvaline, and l-cycloserine was performed by pre-steady-state stopped-flow kinetics. the structure of side chains of the amino acids is impor ... | 2014 | 25398880 |
| pre-steady-state kinetic and structural analysis of interaction of methionine γ-lyase from citrobacter freundii with inhibitors. | methionine γ-lyase (mgl) catalyzes the γ-elimination of l-methionine and its derivatives as well as the β-elimination of l-cysteine and its analogs. these reactions yield α-keto acids and thiols. the mechanism of chemical conversion of amino acids includes numerous reaction intermediates. the detailed analysis of mgl interaction with glycine, l-alanine, l-norvaline, and l-cycloserine was performed by pre-steady-state stopped-flow kinetics. the structure of side chains of the amino acids is impor ... | 2014 | 25398880 |
| dysbiosis and alterations in predicted functions of the subgingival microbiome in chronic periodontitis. | chronic periodontitis is an inflammatory disease of the periodontium affecting nearly 65 million adults in the united states. changes in subgingival microbiota have long been associated with chronic periodontitis. recent culture-independent molecular studies have revealed the immense richness and complexity of oral microbial communities. however, data sets across studies have not been directly compared, and whether the observed microbial variations are consistent across different studies is not ... | 2015 | 25398868 |
| association between maternal periodontitis and preterm and/or low birth weight infants in normal pregnancies. | to relate the periodontal condition with the presence of periodontal bacteria in pregnant that had babies with preterm delivery or/and low weight at birth (ptlbw). | 2016 | 25394610 |
| photodynamic therapy as a novel treatment for halitosis in adolescents: study protocol for a randomized controlled trial. | halitosis is a common problem that affects a large portion of the population worldwide. the origin of this condition is oral in 90% and systemic in 10% of cases. the unpleasant odor is mainly the result of volatile sulfur compounds produced by gram-negative bacteria. however, it has recently been found that anaerobic gram-positive bacteria also produce hydrogen sulfide (h2s) in the presence of amino acids, such as cysteine. light, both with and without the use of chemical agents, has been used t ... | 2014 | 25394474 |
| mirolase, a novel subtilisin-like serine protease from the periodontopathogen tannerella forsythia. | the genome of tannerella forsythia, an etiological factor of chronic periodontitis, contains several genes encoding putative proteases. here, we characterized a subtilisin-like serine protease of t. forsythia referred to as mirolase. recombinant full-length latent promirolase [85 kda, without its signal peptide (sp)] processed itself through sequential autoproteolytic cleavages into a mature enzyme of 40 kda. mirolase latency was driven by the n-terminal prodomain (ntp). in stark contrast to alm ... | 2015 | 25391881 |
| miropin, a novel bacterial serpin from the periodontopathogen tannerella forsythia, inhibits a broad range of proteases by using different peptide bonds within the reactive center loop. | all prokaryotic genes encoding putative serpins identified to date are found in environmental and commensal microorganisms, and only very few prokaryotic serpins have been investigated from a mechanistic standpoint. herein, we characterized a novel serpin (miropin) from the human pathogen tannerella forsythia, a bacterium implicated in initiation and progression of human periodontitis. in contrast to other serpins, miropin efficiently inhibited a broad range of proteases (neutrophil and pancreat ... | 2014 | 25389290 |
| miropin, a novel bacterial serpin from the periodontopathogen tannerella forsythia, inhibits a broad range of proteases by using different peptide bonds within the reactive center loop. | all prokaryotic genes encoding putative serpins identified to date are found in environmental and commensal microorganisms, and only very few prokaryotic serpins have been investigated from a mechanistic standpoint. herein, we characterized a novel serpin (miropin) from the human pathogen tannerella forsythia, a bacterium implicated in initiation and progression of human periodontitis. in contrast to other serpins, miropin efficiently inhibited a broad range of proteases (neutrophil and pancreat ... | 2014 | 25389290 |
| prevalence of six periodontal pathogens in subgingival samples of italian patients with chronic periodontitis. | the aim of this study was to investigate the subgingival prevalence of six periodontal pathogens in 352 italian patients with chronic periodontitis. possible correlations with clinical parameters, age, gender and smoking status were also investigated. at first visit a pooled subgingival plaque sample was obtained for each subject by using the paper-point method. the samples were processed and analysed according to a commercially available quantitative real time polymerase chain reaction assay (m ... | 2014 | 25387289 |
| occurrence of socransky red complex in pregnant women with and without periodontal disease. | to verify the presence of socransky red complex (porphyromonas gingivalis, treponema denticola and tannerella forsythia) and p. intermedia using polymerase chain reaction (pcr) in periodontally healthy pregnant women and pregnant women with periodontal disease, as well as its relation to arterial blood pressure and capillary glycaemia. | 2015 | 25386627 |
| distinct composition of the oral indigenous microbiota in south korean and japanese adults. | a comparison of national surveys on oral health suggested that the population of south korea has a better periodontal health status than that of japan, despite their similar inherent backgrounds. here, we investigated differences in oral bacterial assemblages between individuals from those two countries. to exclude potential effects of oral health condition on the microbiota, we selected 52 korean and 88 japanese orally healthy adults (aged 40-79 years) from the participants of two cohort studie ... | 2014 | 25384884 |
| sensitive real-time pcr detection of pathogenic leptospira spp. and a comparison of nucleic acid amplification methods for the diagnosis of leptospirosis. | bacteria of the genus leptospira, the causative agents of leptospirosis, are categorized into pathogenic and non-pathogenic species. however, the benefit of using a clinical diagnostic that is specific for pathogenic species remains unclear. in this study, we present the development of a real-time pcr (rtpcr) for the detection of pathogenic leptospira (the pathogenic rtpcr), and we perform a comparison of the pathogenic rtpcr with a published assay that detects all leptospira species [the undiff ... | 2014 | 25379890 |
| the effect of conventional mechanical periodontal treatment on red complex microorganisms and clinical parameters in down syndrome periodontitis patients: a pilot study. | periodontal disease (pd) is induced by a complex microbiota, such as porphyromonas gingivalis, tannerella forsythia, and treponema denticola (together called the red complex), which triggers intense inflammatory reaction. down syndrome (ds) individuals demonstrate a high prevalence of pd compared with those who are otherwise chromosomally normal (euploids). this pilot study aimed to evaluate the effect of non-surgical periodontal treatment in ds chronic periodontitis patients on clinical and mic ... | 2015 | 25367612 |
| building carbon-carbon bonds using a biocatalytic methanol condensation cycle. | methanol is an important intermediate in the utilization of natural gas for synthesizing other feedstock chemicals. typically, chemical approaches for building c-c bonds from methanol require high temperature and pressure. biological conversion of methanol to longer carbon chain compounds is feasible; however, the natural biological pathways for methanol utilization involve carbon dioxide loss or atp expenditure. here we demonstrated a biocatalytic pathway, termed the methanol condensation cycle ... | 2014 | 25355907 |
| isolation of digital dermatitis treponemes from hoof lesions in wild north american elk (cervus elaphus) in washington state, usa. | since 2008, a large increase in the numbers of cases of lameness have been seen in wild north american elk (cervus elaphus) from washington state, usa. the most recent cases manifested as foot lesions similar both clinically and pathologically to those seen in digital dermatitis (dd) in cattle and sheep, a disease with a bacterial etiopathogenesis. to determine whether the same bacteria considered responsible for dd are associated with elk lameness, lesion samples were subjected to bacterial iso ... | 2014 | 25355757 |
| isolation of digital dermatitis treponemes from hoof lesions in wild north american elk (cervus elaphus) in washington state, usa. | since 2008, a large increase in the numbers of cases of lameness have been seen in wild north american elk (cervus elaphus) from washington state, usa. the most recent cases manifested as foot lesions similar both clinically and pathologically to those seen in digital dermatitis (dd) in cattle and sheep, a disease with a bacterial etiopathogenesis. to determine whether the same bacteria considered responsible for dd are associated with elk lameness, lesion samples were subjected to bacterial iso ... | 2014 | 25355757 |
| metabolic engineering of methylobacterium extorquens am1 for 1-butanol production. | butanol is a promising next generation fuel and a bulk chemical precursor. although clostridia are the primary industrial microbes for the fermentative production of 1-butanol, alternative engineered hosts have the potential to generate 1-butanol from alternative carbon feedstocks via synthetic metabolic pathways. methylobacterium extorquens am1, a facultative methylotrophic α-proteobacterium, is a model system for assessing the possibility of generating products such as 1-butanol from one-carbo ... | 2014 | 25349627 |
| the association between the upper digestive tract microbiota by homim and oral health in a population-based study in linxian, china. | bacteria affect oral health, but few studies have systematically examined the role of bacterial communities in oral diseases. we examined this relationship in a large population-based chinese cancer screening cohort. | 2014 | 25348940 |
| complete genome sequence of the oral spirochete bacterium treponema putidum strain omz 758t (atcc 700334t). | the oral spirochete bacterium treponema putidum inhabits human periodontal niches. the complete genome sequence of the omz 758(t) (atcc 700334(t)) strain of this species was determined, revealing a 2,796,913-bp chromosome, with a g+c content of 37.30% and a single plasmid (ptpu1; 3,649 bp) identical to pts1 from treponema denticola. | 2014 | 25342686 |
| effect of non-surgical periodontal treatment on short chain fatty acid levels in gingival crevicular fluid of patients with generalized aggressive periodontitis. | short chain fatty acids (scfas) play important roles in periodontal diseases. however, the concentrations of scfas in gingival crevicular fluid of patients with aggressive periodontitis are not known. the aim of this intervention study was to investigate the influences of non-surgical periodontal therapy on levels of scfas in the gingival crevicular fluid of patients with generalized aggressive periodontitis (g-agp), and analyze the concentrations of scfas in sites with or without the detected p ... | 2014 | 25340203 |
| association between the presence of treponema denticola and reduced levels of antiatherogenic high density lipoprotein in periodontitis. | dyslipidemia, a disorder in the levels of cholesterol or lipoproteins in the plasma, is a feature of atherosclerosis, leading to cardiovascular disease. the objective of this study was to evaluate whether tanerella forsythia and treponema denticola are related to reduced levels of antiatherogenic high density lipoprotein (hdl). | 2015 | 25328922 |
| intraindividual variation in core microbiota in peri-implantitis and periodontitis. | the oral microbiota change dramatically with each part of the oral cavity, even within the same mouth. nevertheless, the microbiota associated with peri-implantitis and periodontitis have been considered the same. to improve our knowledge of the different communities of complex oral microbiota, we compared the microbial features between peri-implantitis and periodontitis in 20 patients with both diseases. although the clinical symptoms of peri-implantitis were similar to those of periodontitis, ... | 2014 | 25308100 |
| relationship of clinical and microbiological variables in patients with type 1 diabetes mellitus and periodontitis. | the aim of the study was to analyze how metabolic control of type 1 diabetes is related to clinical and microbiological periodontal parameters. | 2014 | 25294115 |
| influence of highly-active antiretroviral therapy on the subgingival biofilm in hiv-infected patients. | highly-active antiretroviral therapy (haart) has been associated with alterations in subgingival biofilm and periodontal disease. the purpose of the present study was to investigate the association between different haart regimens and the prevalence of periodontal pathogens in hiv-infected patients with chronic periodontitis. | 2016 | 25283543 |
| selective microbial genomic dna isolation using restriction endonucleases. | to improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific dna binding proteins. as an example, we use dpni immobilized on magnetic beads. the ten minute extraction technique allows specific binding of genomes containing the dpni gm6atc motif common in the genomic dna of many bacteria including γ-proteobacteria. using synthetic genome mixtures, we demonstrate 80% recovery of escherichia coli genomic dna even when only femtogram qua ... | 2014 | 25279840 |
| role of porphyromonas gingivalis gingipains in multi-species biofilm formation. | periodontal diseases are polymicrobial diseases that cause the inflammatory destruction of the tooth-supporting (periodontal) tissues. their initiation is attributed to the formation of subgingival biofilms that stimulate a cascade of chronic inflammatory reactions by the affected tissue. the gram-negative anaerobes porphyromonas gingivalis, tannerella forsythia and treponema denticola are commonly found as part of the microbiota of subgingival biofilms, and they are associated with the occurren ... | 2014 | 25270662 |
| screening and development of dna aptamers specific to several oral pathogens. | aptamers are composed of single-stranded oilgonucleotides that can selectively bind desired molecules. it has been reported that rna or dna could act as not only a genetic messenger but also a catalyst in metabolic pathways. rna aptamers (average sizes 40-50 bp) are smaller than antibodies and have strong binding capacities to target molecules, similar to antigen-antibody interactions. once an aptamer was selected, it can be readily produced in large quantities at low cost. the objectives of thi ... | 2015 | 25269812 |
| polymerase chain reaction as a prospect for the early diagnosis and prediction of periodontal diseases in adolescents. | this study was to determine the markers representative of pathogenic periodontal microflora [prevotella intermedia (p.i), tannerella forsythia (t.f) [bacteroides forsythus], treponema denticola (t.d), actinobacillus actinomycetemcomitans (a.a), porphyromonas gingivalis (p.g)] in the dental plaque of adolescents with various degrees of severity of periodontium inflammation. | 2015 | 25266040 |
| lack of cathelicidin processing in papillon-lefèvre syndrome patients reveals essential role of ll-37 in periodontal homeostasis. | loss-of-function point mutations in the cathepsin c gene are the underlying genetic event in patients with papillon-lefèvre syndrome (pls). pls neutrophils lack serine protease activity essential for cathelicidin ll-37 generation from hcap18 precursor. | 2014 | 25260376 |
| l-methionase: a therapeutic enzyme to treat malignancies. | cancer is an increasing cause of mortality and morbidity throughout the world. l-methionase has potential application against many types of cancers. l-methionase is an intracellular enzyme in bacterial species, an extracellular enzyme in fungi, and absent in mammals. l-methionase producing bacterial strain(s) can be isolated by 5,5'-dithio-bis-(2-nitrobenzoic acid) as a screening dye. l-methionine plays an important role in tumour cells. these cells become methionine dependent and eventually fol ... | 2014 | 25250324 |
| prevalence of pseudomonas aeruginosa and acinetobacter spp. in subgingival biofilm and saliva of subjects with chronic periodontal infection. | p. aeruginosa and acinetobacter spp. are important pathogens associated with late nosocomial pneumonia in hospitalized and institutionalized individuals. the oral cavity may be a major source of these respiratory pathogens, particularly in the presence of poor oral hygiene and periodontal infection. this study investigated the prevalence of p. aeruginosa and acinetobacter spp. in subgingival biofilm and saliva of subjects with periodontal disease or health. samples were obtained from 55 periodon ... | 2014 | 25242933 |
| next-generation models of human cardiogenesis via genome editing. | cardiogenesis is one of the earliest and most important steps during human development and is orchestrated by discrete families of heart progenitors, which build distinct regions of the fetal heart. for the past decade, a lineage map for the distinct subsets of progenitors that generate the embryonic mammalian heart has begun to lay a foundation for the development of new strategies for rebuilding the adult heart after injury, an unmet clinical need for the vast majority of patients with end-sta ... | 2014 | 25237142 |
| association between polycystic ovary syndrome, oral microbiota and systemic antibody responses. | polycystic ovary syndrome (pcos) is a hormonal disorder of women that not only is the leading cause of infertility but also shows a reciprocal link with oral health. this study aimed to investigate the hypothesis that the levels of putative periodontal pathogens in saliva and their antibody response in serum are elevated in pcos, compared to systemic health. a total of 125 women were included in four groups; 45 women with pcos and healthy periodontium, 35 women with pcos and gingivitis, 25 syste ... | 2014 | 25232962 |
| bacterial community composition of chronic periodontitis and novel oral sampling sites for detecting disease indicators. | periodontitis is an infectious and inflammatory disease of polymicrobial etiology that can lead to the destruction of bones and tissues that support the teeth. the management of chronic periodontitis (cp) relies heavily on elimination or at least control of known pathogenic consortia associated with the disease. until now, microbial plaque obtained from the subgingival (subg) sites has been the primary focus for bacterial community analysis using deep sequencing. in addition to the use of subg p ... | 2014 | 25225610 |
| oral fluid based biomarkers in periodontal disease: part 1. saliva. | traditional clinical measurements such as probing pocket depth, bleeding on probing, clinical attachment loss; plaque index and radiographs used for periodontal diagnosis are often of limited usefulness as they are indicators of previous periodontal disease rather than present disease activity. a literature search was carried out to find out all the available tests that indicate periodontal disease markers in saliva. all major databases were searched to compile the information on published repor ... | 2014 | 25214743 |
| relationship between gingival bleeding and anaerobic periodontal infection assessed by bana (n-benzoyl-dl-arginine-β-napthylamide) assay. | since periodontal diseases are primarily inflammatory in nature, the ability to detect inflammatory lesions in gingival tissue is essential for the diagnosis and monitoring of changes in gingival status. gingival bleeding has been used as a reliable and objective clinical parameter not only for the evaluation of inflammatory conditions of periodontium, but also in the detection of its activity and progression. the gingival bleeding index (loe) is based on the presence or absence of gingival blee ... | 2014 | 25210389 |
| strategies for the inhibition of gingipains for the potential treatment of periodontitis and associated systemic diseases. | gingipains are the major virulence factors of porphyromonas gingivalis, the main periodontopathogen. it is expected that inhibition of gingipain activity in vivo could prevent or slow down the progression of adult periodontitis. to date, several classes of gingipain inhibitors have been recognized. these include gingipain n-terminal prodomains, synthetic compounds, inhibitors from natural sources, antibiotics, antiseptics, antibodies, and bacteria. several synthetic compounds are potent gingipai ... | 2014 | 25206939 |
| [comparison of the effects of ginkgo biloba extract and minocycline hydrochlovide on periodontitis]. | to compare the clinical effect of ginkgo biloba extract gel (ginkgo biloba extract,egb) and minocycline hydrochloride (periocline) on periodontitis and their inhibition on putative periodontal pathogens. | 2014 | 25199931 |
| identification of diversity-generating retroelements in human microbiomes. | diversity-generating retroelements (dgrs) are a unique family of retroelements that confer selective advantages to their hosts by accelerating the evolution of target genes through a specialized, error-prone, reverse transcription process. first identified in a bordetella phage (bpp-1), which mediates the phage tropism specificity by generating variability in an involved gene, dgrs were predicted to be present in a larger collection of viral and bacterial species. a minimal dgr system is compris ... | 2014 | 25196521 |
| definition, etiology, prevention and treatment of peri-implantitis--a review. | peri-implant inflammations represent serious diseases after dental implant treatment, which affect both the surrounding hard and soft tissue. due to prevalence rates up to 56%, peri-implantitis can lead to the loss of the implant without multilateral prevention and therapy concepts. specific continuous check-ups with evaluation and elimination of risk factors (e.g. smoking, systemic diseases and periodontitis) are effective precautions. in addition to aspects of osseointegration, type and struct ... | 2014 | 25185675 |
| a crispr view of development. | the crispr (clustered regularly interspaced short palindromic repeat)-cas9 (crispr-associated nuclease 9) system is poised to transform developmental biology by providing a simple, efficient method to precisely manipulate the genome of virtually any developing organism. this rna-guided nuclease (rgn)-based approach already has been effectively used to induce targeted mutations in multiple genes simultaneously, create conditional alleles, and generate endogenously tagged proteins. illustrating th ... | 2014 | 25184674 |
| microbial peptidyl-prolyl cis/trans isomerases (ppiases): virulence factors and potential alternative drug targets. | initially discovered in the context of immunomodulation, peptidyl-prolyl cis/trans isomerases (ppiases) were soon identified as enzymes catalyzing the rate-limiting protein folding step at peptidyl bonds preceding proline residues. intense searches revealed that ppiases are a superfamily of proteins consisting of three structurally distinguishable families with representatives in every described species of prokaryote and eukaryote and, recently, even in some giant viruses. despite the clear-cut ... | 2014 | 25184565 |
| a homolog of glyceraldehyde-3-phosphate dehydrogenase from riemerella anatipestifer is an extracellular protein and exhibits biological activity. | riemerella anatipestifer is the causative agent of septicemia anserum exsudativa in ducks. its pathogenesis and virulence factors are still unclear. the glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase (gapdh), an anchorless and multifunctional protein on the surface of several pathogenic microorganisms, is involved in virulence and adhesion. whether homologs of gapdh exist, and display similar characteristics in r. anatipestifer (ragapdh) has not been determined. in our research, the ... | 2014 | 25183032 |
| metabolic engineering of microorganisms for the production of higher alcohols. | due to the increasing concerns about limited fossil resources and environmental problems, there has been much interest in developing biofuels from renewable biomass. ethanol is currently used as a major biofuel, as it can be easily produced by existing fermentation technology, but it is not the best biofuel due to its low energy density, high vapor pressure, hygroscopy, and incompatibility with current infrastructure. higher alcohols, including 1-propanol, 1-butanol, isobutanol, 2-methyl-1-butan ... | 2014 | 25182323 |
| the presence of cariogenic and periodontal pathogens in the oral cavity of one-year-old infants delivered pre-term with very low birthweights: a case control study. | recently, the dental literature has focused mainly on the microbial colonization of healthy full-term infants and their mothers or caretakers. however, oral microbial acquisition by premature infants has not been adequately investigated, and the correlation between pre-term birth and the presence of cariogenic and periodontal pathogens has not been determined. the aim of this study was to identify the presence and quantities of representative cariogenic and periodontal pathogens in the oral cavi ... | 2014 | 25178742 |
| a pathogenic trace of tannerella forsythia - shedding of soluble fully active tumor necrosis factor α from the macrophage surface by karilysin. | tannerella forsythia is implicated as a pathogen causing chronic and aggressive periodontitis. however, its virulence factors, including numerous putative proteases, are mostly uncharacterized. karilysin is a newly described matrix metalloprotease-like enzyme of t. forsythia. since pathogen-derived proteases may affect the host defense system via modulation of the cytokine network, the aim of this study was to determine the influence of karilysin on tumor necrosis factor-α (tnf-α). the results s ... | 2014 | 25175980 |
| periodontal bacteria in human carotid atherothrombosis as a potential trigger for neutrophil activation. | epidemiological, biological and clinical links between periodontal and cardiovascular diseases are now well established. several human studies have detected bacterial dna corresponding to periodontal pathogens in cardiovascular samples. intraplaque hemorrhage has been associated with a higher risk of atherosclerotic plaque rupture, potentially mediated by neutrophil activation. in this study, we hypothesized that plaque composition may be related to periodontal pathogens. | 2014 | 25173070 |
| comparative genome analysis and identification of competitive and cooperative interactions in a polymicrobial disease. | polymicrobial diseases are caused by combinations of multiple bacteria, which can lead to not only mild but also life-threatening illnesses. periodontitis represents a polymicrobial disease; porphyromonas gingivalis, treponema denticola and tannerella forsythia, called 'the red complex', have been recognized as the causative agents of periodontitis. although molecular interactions among the three species could be responsible for progression of periodontitis, the relevant genetic mechanisms are u ... | 2014 | 25171331 |
| comparative genome analysis and identification of competitive and cooperative interactions in a polymicrobial disease. | polymicrobial diseases are caused by combinations of multiple bacteria, which can lead to not only mild but also life-threatening illnesses. periodontitis represents a polymicrobial disease; porphyromonas gingivalis, treponema denticola and tannerella forsythia, called 'the red complex', have been recognized as the causative agents of periodontitis. although molecular interactions among the three species could be responsible for progression of periodontitis, the relevant genetic mechanisms are u ... | 2014 | 25171331 |
| pilot study on the clinical and microbiological effect of subgingival glycine powder air polishing using a cannula-like jet. | to assess the efficacy of subgingival glycine powder air polishing (gpap) during supportive periodontal therapy (spt). | 2015 | 25156125 |
| dentilisin involvement in coaggregation between treponema denticola and tannerella forsythia. | periodontitis arises from a biofilm consisting of gram-negative anaerobic rods and spirochetes. porphyromonas gingivalis, treponema denticola, and tannerella forsythia, termed the red complex, have been co-isolated with high frequency from chronic periodontitis lesions, and these microorganisms are thought to be major pathogens of the disease. coaggregation is an important strategy in the colonization of dental plaque biofilm by these bacteria. in the present study, we investigated the coaggrega ... | 2014 | 25152229 |
| quantification of periodontal pathogens cell counts by capillary electrophoresis. | gingivitis is a highly prevalent periodontal disease around the worldwide. porphyromonas gingivalis (p.g), treponema denticola (t.d) and tannerela forsythia (t.f) were considered to be three important periodontal pathogens related to gingivitis, and research shows that the counts of periodontal pathogen cells in the patients before, during, and after fixed orthodontic appliance therapy were quite different. we proposed a simple method to extract the periodontal pathogens from the periodontal poc ... | 2014 | 25145561 |
| serum monocyte chemoattractant protein-1 is a biomarker in patients with diabetes and periodontitis. | the role of serum monocyte chemoattractant protein-1 (mcp-1) as a biomarker of periodontitis is well documented; however, its role in diabetic patients with periodontitis is unknown. | 2014 | 25143907 |