Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| estimates of quantitative genetic parameters of immunological traits in the chicken. | three in vivo assays were used to measure the immunocompetence of chickens in two generations of a selection experiment. the obtained data were used to estimate the variance components for sire and dam for antibody production to pasteurella multocida and mycoplasma gallisepticum vaccines, for t-cell-mediated immunity evaluated by a phytohemagglutinin wing web assay, and for clearance of foreign particles from the circulatory system. heritabilities of and genetic correlations among these immunolo ... | 1991 | 1956847 |
| comparison of a modified edward-type medium and a modified sp4-type medium for primary isolation of mycoplasma gallisepticum (mg) from chickens vaccinated with the f strain of mg. | the efficacy of two media, an edward-type medium (epj) and a modified sp4-type medium (sp4-ps), were compared for primary isolation of mycoplasma gallisepticum (mg) from commercial layer chickens (n = 58) vaccinated with the live f strain of mg. three groups of chickens that differed in the interval after vaccinal exposure to the f strain (32, 41, and 102 weeks) were studied at necropsy. mycoplasma isolation was attempted from the trachea, sinus, and cloaca using lavage and swab techniques but w ... | 1991 | 1953581 |
| health survey of backyard poultry and other avian species located within one mile of commercial california meat-turkey flocks. | a survey was conducted to characterize domestic and exotic bird populations, estimate seroprevalence to selected disease agents, and describe health management practices on 62 premises containing "backyard" flocks located within one mile of 22 commercial california meat-turkey flocks participating in national animal health monitoring system (nahms). chickens were present on 56 backyard premises and turkeys on seven. antibodies were identified against mycoplasma gallisepticum, m. synoviae, m. mel ... | 1991 | 1854324 |
| comparison of mycoplasma gallisepticum strains and identification of immunogenic integral membrane proteins with triton x-114 by immunoblotting. | pooled chicken antisera from 33 and 77 days post mycoplasma gallisepticum strain r contact-exposure reacted with cell proteins of 19 m. gallisepticum strains. these pooled antisera reacted with more proteins and with greater intensity to reference strains (r, pg31, s6, and a5969) and nine field strains than they did with six other field strains including three (703, 503, and 730) that have been described as serological variants. following extraction with triton x-114 the majority of immunogenic ... | 1991 | 1771754 |
| an enzyme-linked immunosorbent assay for the detection of specific igg antibody to mycoplasma gallisepticum in sera and tracheobronchial washes. | a sensitive indirect elisa is reported for the detection and quantitation of specific igg to mycoplasma gallisepticum (mg) in sera and tracheobronchial washes (tbw) of mg-infected chickens. the sensitivity of the assay was ensured by the use of mouse monoclonal antibody to chicken igg bound to a prospective anti-mg containing sample that was complexed with mg antigen immobilized on a solid phase. the level of specific igg antibody in a test sample was detected by using peroxidase-conjugated goat ... | 1992 | 1569215 |
| comparison of immunity induced with a mycoplasma gallisepticum bacterin between high- and low-responder lines of chickens. | chickens of the high-responder line gsp and low-responder line bm-c, which had been known to have different antibody responses to mycoplasma gallisepticum (mg) antigen, were immunized by intramuscular injection and by subsequent intratracheal instillation of mg bacterin. they were then challenged with the pathogenic strain sas of mg. the preventive effects of local antibodies detectable in the trachea, saliva, and lacrima were compared between the two lines of chickens. the local antibody respon ... | 1992 | 1567299 |
| combination of immunofluorescence and immunoperoxidase techniques for serotyping mixtures of mycoplasma species. | we describe a method for the simultaneous identification of up to three mycoplasma species by the use of contrast-labeled fluorescent antibodies against two species and peroxidase-labeled antibody against a third species. the procedure enabled the rapid identification of colonies of three artificially mixed avian mycoplasma species on agar blocks and also mixtures of species in cultures from naturally infected chickens. furthermore, it was possible to quantitate the components of a mixture of my ... | 1992 | 1537910 |
| simultaneous treatment of chickens with salinomycin and tiamulin in feed. | laboratory and field experiments involving more than 100,000 birds were performed to assess the effect of simultaneous in-feed medication of chickens with salinomycin and tiamulin at various concentrations. in an artificial infection study with mycoplasma gallisepticum, low levels of tiamulin (10-40 ppm) did not induce signs of ionophore intoxication with salinomycin at 60 ppm in the feed, whereas levels of 50 ppm caused early signs with a mild growth depression. a level of 20 ppm gave a maximum ... | 1992 | 1533113 |
| sequential intracoelomic and intrabursal immunization of chickens with inactivated mycoplasma gallisepticum bacterin and iota carrageenan adjuvant. | chickens immunized by sequential intracoelomic (analogous to intraperitoneal route in mammals) and intrabursal (i.c./i.b.) routes with inactivated mycoplasma gallisepticum (mg) bacterin mixed with 0.2% iota carrageenan (icgn) as an adjuvant were resistant to airsacculitis induced by a subsequent aerosol challenge with virulent r strain mg. in contrast, immunization by the i.c./i.b. routes with inactivated bacterin without the adjuvant or with 0.2% icgn did not confer significant protection. chic ... | 1992 | 1523875 |
| mycoplasma gallisepticum vaccination-challenge: an egg-production model. | specific-pathogen-free layer hens in maximum lay were exposed by aerosol to a broth culture of mycoplasma gallisepticum r' strain. egg-production loss of greater than 50% was evident 7-14 days following challenge of unvaccinated chickens, with a gradual recovery during the next several days. various vaccine preparations were tested to determine the effect in the model. all vaccinated chickens exhibited significantly (p < or = 0.05) lower egg-production loss than the unvaccinated controls. the mo ... | 1992 | 1485879 |
| [molecular biological differences between strains of mycoplasma gallisepticum]. | differences in virulence of two mycoplasma gallisepticum strains, s6 and a5969, are confirmed in experiments with chickens. macromolecular discrepancies detected between these two strains are concerning the genomic size, electrophoretic spectra of dna and proteins. cross immunoblotting data with polyclonal and monoclonal antibodies reveal major immunogens of protein nature in both the strains. homologous proteins with different electrophoretic mobility are detected in other four m. gallisepticum ... | 1992 | 1455557 |
| [tubular structures in mycoplasma gallisepticum and the localization of a tubulin-like protein]. | in all the strains of m. gallisepticum investigated, a protein with apparent molecular weight 40 kda was revealed by immunoblotting with polyclonal anti-calf brain tubulin antibodies and monoclonal anti-chicken alpha-tubulin antibodies. in other 8 investigated mycoplasma species no positive reactions with the same antibodies were found. the m. gallisepticum cells were examined under electron microscope on fine serial sections and on some sections going at different angles to the long cell axis. ... | 1992 | 1440927 |
| effects of ascorbic acid on stress and disease in chickens. | white leghorn chickens were given feed containing 100 mg of ascorbic acid (aa)/kg. one day later, treated chickens and a similar group of unmedicated control chickens were chilled for 1 hour at 6 c, exposed to an unusual sound, fasted, or subjected to rough handling. heterophil:lymphocyte (h:l) ratios were determined one day later. the aa-treated birds had significantly lower h:l ratios than untreated controls. chickens that received a diet containing aa had lower h:l ratios than controls (0.86 ... | 1992 | 1417599 |
| the effect of including anti-ig sera in the haemagglutination inhibition test for mycoplasma gallisepticum. | addition of anti-immunoglobulin m (anti-igm), g (anti-igg) and a (anti-iga) sera to the haemagglutination-inhibition (hi) test (anti-ig hi test) for mycoplasma gallisepticum resulted in 2- to 8-fold increases in the hi titres. on investigating the anti-ig hi reaction using igm and igg antibodies separated by affinity chromatography, it was confirmed that, in the enhanced hi titres, specificity existed between the chicken ig classes having antibody activity and the antisera used in the test. four ... | 1992 | 1413478 |
| localization of an immunodominant 64 kda lipoprotein (lp 64) in the membrane of mycoplasma gallisepticum and its role in cytadherence. | a 64 kda lipoprotein (lp 64) haemagglutinin (pi 4.9-5.0) was isolated from the membrane of mycoplasma gallisepticum. triton x-114 phase partitioning has demonstrated that the hydrophobic nature of this haemagglutinin is due to a lipid portion of the molecule. autoradiography of [3h]-palmitate-labelled m. gallisepticum revealed the presence of several additional lipoproteins. immunoelectron microscopy demonstrated the localization of lp 64 to the base of the terminal structure. densitometric scan ... | 1992 | 1406251 |
| evaluation of a mycoplasma gallisepticum strain exhibiting reduced virulence for prevention and control of poultry mycoplasmosis. | two experiments were conducted to evaluate the virulence and vaccination efficacy of a mycoplasma gallisepticum (mg) isolate designated mg intervet 6/85. virulence of the strain was determined by evaluation of airsacculitis scores following aerosol exposure to the isolate before and after 10 sequential passes in either commercial broiler chickens or commercial turkeys. two-week-old specific-pathogen-free chickens were vaccinated by aerosol exposure. the birds were challenged with the r' strain o ... | 1992 | 1385698 |
| characterization of a major hemagglutinin protein from mycoplasma gallisepticum. | mycoplasma gallisepticum cell membranes were used to immunize mice to produce monoclonal antibodies to cell surface proteins. three monoclonal antibodies were chosen for further characterization. all three reacted in immunoblots with an m. gallisepticum protein band of m(r) approximately 67,000 (designated pmga). by using immunoelectron microscopy, pmga was shown to be located on the cell surface. when m. gallisepticum whole cells were treated with up to 250 micrograms of trypsin per ml for 30 m ... | 1992 | 1379991 |
| successful treatment of mycoplasmosis in layer chickens with single dose therapy. | the efficacy of treatment with single dose administration of 5 drugs at different dosages to layer hens naturally infected with mycoplasma gallisepticum was studied. the drugs were tiamulin, which was administered orally, tylosin (parenterally and orally), spiramycin (orally), long-acting oxytetracycline (parenterally) and tylosindihydrostreptomycin (parenterally). cure was assessed by the absence of nasal discharge. the cure rate was significantly higher (p less than 0.05) in treated hens than ... | 1992 | 1379426 |
| demonstration of the genetic stability of a mycoplasma gallisepticum strain following in vivo passage. | a mycoplasma gallisepticum strain designated 6/85 (mgi) exhibiting reduced virulence for both chickens and turkeys was sequentially passaged 10 times in each species. dna extracted from organisms before passage and those isolated after the third, sixth, and 10th passages was studied by restriction endonuclease dna analysis using bamhi, bglii, ecori, hindiii, and psti endonucleases. the virulent-type strain designated s6 was used as a comparison. comparison of dna fragment patterns of mgi and s6 ... | 1992 | 1329707 |
| clinical, cultural, and serologic observations of avian mycoplasmosis in two chicken breeder flocks. | two chicken breeding flocks from different breeding lines were studied serologically and culturally for mycoplasma gallisepticum (mg) throughout their growing and laying period. infection was proven by successful isolation of mg from both breeders and progeny originating from these two flocks. observations of these flocks which were serologically and culturally negative for mycoplasma synoviae (ms) further disclosed that: 1) negative plate tests of large numbers of day-old progeny may sometimes ... | 1976 | 1259662 |
| a comparison of commercial mycoplasma gallisepticum antigens in the rapid serum agglutination test. | twenty-three chickens, aged 9 weeks, were housed in contact with a similar group of chickens which were infected with m. gallisepticum. the onset and development of the serum agglutinin responses which were detected by 3 commercial antigens using rapid serum agglutination tests up to 20 weeks of age, differed greatly both between the infected and in-contact groups and, within the latter group, between the antigens. few reactions, which were weak and transient, were detected in 2 similar groups o ... | 1975 | 1222006 |
| dissociation of mycoplasma gallisepticum membranes with lithium diiodosalicylate and isolation of glycoprotein. | m. gallisepticum membranes were treated with 0.3m lithium diiodosalicylate (lis) and, on average, 43% of the original membrane proteins were extracted. the extract contained particles with a sedimentation coefficient of 13s and some aggregated proteins. this lis extract was immunogenic, stimulating the production of haemagglutination-inhibiting, growth-inhibiting and precipitating antibodies in rabbits. it was devoid of haemagglutinating (ha) activity for chicken erythrocytes but did inhibit the ... | 1975 | 1211714 |
| interference of mycoplasma gallisepticum with multiplication of newcastle disease virus in chickens. | 1975 | 1164323 | |
| in vivo efficacy of 81.723 hfu, a new pleuromutilin derivative against experimentally induced airsacculitis in chicks and turkey poults. | the efficacy of the pleuromutilin derivative 81.723 hfu was tested in chicks and turkey poults by experimentally infecting them with mycoplasma gallisepticum. the data were treated to obtain the mean effective doses, and these were compared to those obtained with tylosin tartrate. the compounds were administered either by ingluvial catheter or via the drinking water. therapy was started on the day of inoculation or 7 days thereafter, respectively. the experiments showed that the compound 81.723 ... | 1975 | 1147586 |
| single and mixed infections of avian infectious bronchitis virus and mycoplasma gallisepticum. | comparative studies of the clinical signs, pathological changes, multiplication of the pathogens and serological responses were made of groups of chickens infected with either or both avian infectious bronchitis virus and mycoplasma gallisepticum. electron microscopic examination of thin sections of infected tracheas showed that the multiplication of m. gallisepticum was greatly enhanced in the tracheas of chickens which had been previously or simultaneously infected with avian infectious bronch ... | 1975 | 1126565 |
| a field trial, using killed-mycoplasma gallisepticum vaccine to protect against chicken respiratory mycoplasmosis. | in a field trial with chickens, the efficacy of killed mycoplasma gallisepticum vaccine against respiratory mycoplasmosis was determined. broiler chickens were vaccinated by aerosol administration at 3, 14, and 25 days of age, and observations were made of survival rate, body weight gain ratio, macroscopic lesion grade of airsacculitis, isolation of organisms, serologic test results, histopathologic changes, and clinical signs. inhalation of the vaccinal aerosol did not influence livability and ... | 1975 | 1111388 |
| immunization of chickens against mycoplasma gallisepticum infection. | 1975 | 1092221 | |
| further observations on the use of a bivalent bacterin against haemophilus gallinarum. | chickens vaccinated with two doses of a bivalent haemophilus gallinarum bacterin were protected against seven strains of the organism. vaccinated and unvaccinated birds with antibody to mycoplasma gallisepticum had increased hi titers when challenged with h. gallinarum. birds positive for antibody to mycoplasma gallisepticum which were not challenged showed no increase in hi titer. | 1976 | 962761 |
| attachment of mycoplasma gallisepticum to the tracheal epithelium of fowls. | an electron microscopical study of fowl trachea infected with mycoplasma gallisepticum showed slight to moderate changes at the cellular level. mycoplasmas were demonstrated extra-cellularly and showed triple membranes, intracytoplasmic spaces, fibrillar nuclear areas and blebs. blebs were seen attached to the plasma membrane of the epithelial cells. | 1977 | 860098 |
| [identification of mycoplasma gallisepticum with a hemadsorption inhibition test]. | 1977 | 842193 | |
| use of the agar-gel precipitin test to evaluate broiler breeder and commercial layer flocks for mycoplasma gallisepticum infection. | in the absence of mycoplasma gallisepticum (mg) isolation, the importance of agar-gel precipitin (agp) tests along with microhemagglutination-inhibition (mhi) and serum plate agglutination (spa) tests for the evaluation of poultry flocks for mg infection was demonstrated. a good correlation between agp, mhi, and spa tests for the early confirmation of mg in a flock was observed, except for the breeder flocks from which wvu 907 was isolated. the serums of such breeder flocks were positive for spa ... | 1976 | 822817 |
| effect of tiamulin in chickens and turkeys infected experimentally with avian mycoplasma. | tiamulin was compared, under a variety of experimental conditions, with 4 water-soluble antibiotic preparations available commercially for the control and treatment of mycoplasmal infections in poultry. in chickens, tiamulin was more effective than the reference antibiotics in preventing and eradicating airsacculitis caused by mycoplasma gallisepticum and preventing airsacculitis and synovitis caused by m. synoviae. in turkeys, tiamulin and tylosin were equally active in preventing airsacculitis ... | 1978 | 749887 |
| suppression of immunoresponses to haemophilus gallinarum with nonviable mycoplasma gallisepticum in chickens. | the suppressive effect of mycoplasma gallisepticum (mg) on haemophilus gallinarum (hg) immune response was shown. antibody response to hg was highly suppressed when chickens were inoculated intramuscularly with hg-mg combined bacterin. findings were similar in chickens injected intramuscularly with hg and mg bacterin separately at adjacent sites. no immunosuppressive effect was recognized when injections with hg and mg bacterins were in the left and right thigh muscles, respectively, or from int ... | 1978 | 749885 |
| the isolation of lentogenic strains of newcastle disease virus in australia. | twelve isolations of newcastle disease virus were made from 77 clinical samples from chickens from conjunctivitis, respiratory disease, proventriculitis and bursal atrophy. nine of the isolations were made from chickens with conjunctivitis. the viruses were identified as newcastle disease virus by inhibition of their haemagglutinins with specific antiserum to newcastle disease virus. the viruses failed to kill chicken embryos after inoculation into the allantoic cavity and they were judged to be ... | 1978 | 687276 |
| infectious coryza: preventing complicated coryza with haemophilus gallinarum and mycoplasma gallisepticum bacterins. | three types of infectious coryza were produced in unvaccinated chickens by challenge inoculums containing different combinations of haemophilus gallinarum (hg) and mycoplasma gallisepticum (mg). monovalent and combination bacterins of hg and mg were tested to determine their efficacy against chronic complicated infectious coryza. challenge exposure of vaccinates with mg and hg showed protection against the hg component to be immunotype-specific. some protection against complicated coryza resulte ... | 1978 | 646755 |
| adaptation of chickens to their handler, and experimental results. | chickens were adapted to their handler before the experiment. adapted birds produced more antibody, had more blood protein, gained more weight, and were more resistant to mycoplasma gallisepticum than unadapted birds. the antibody response to sheep red blood cells was not reduced for fasting for 48 hours in adapted birds but was in unadapted birds. differences in antibody titers, blood protein, and weight gains between controls and birds fed 80 ppm of deoxycorticosterone could be demonstrated on ... | 1979 | 526204 |
| an ultrastructural study on the interaction of mycoplasma gallisepticum with the chicken tracheal epithelium. | seven-day-old chickens wee intratracheally inoculated with mycoplasma gallisepticum. the tracheas collected 6 and 14 days after chickens were inoculated were subjected to titration of mycoplasma and examination by light and electron microscopy. the mycoplasma organisms grew well; 10(7) to 10(8) color-changing units in a milligram of tissue were determined. tracheal lesions occurred in close association with the presence of mycoplasmas and were characterized by degeneration of the epithelial cell ... | 1979 | 507485 |
| haemadsorption inhibition test for the identification of mycoplasma gallisepticum and mycoplasma synoviae. | colonies of the avian mycoplasma strains mycoplasma gallisepticum s6 and mycoplasma synoviae wvu 1853 and two mycoplasma synoviae isolates from this laboratory were shown to be haemadsorption positive for chicken erythrocytes. three mycoplasma synoviae isolates from this laboratory proved to be haemadsorption and haemagglutination negative. the haemadsorption of the mycoplasma colonies mentioned above was inhibited with specific antisera of either high or low titre. no cross-inhibition was obser ... | 1979 | 473153 |
| isolation and identification of avian mycoplasmas in singapore. | two hundred and forty batches of chickens with chronic respiratory syndrome were tested for mycoplasmas. one hundred and five batches (43.8%) were found to have mycoplasmosis. a total of 110 isolates of mycoplasma was cultured, of which nine isolates were identified as mycoplasma gallisepticum, 48 avian sero-group d, 45 m. gallinarum, one m. iners and seven unclassified. 2. identification of the mycoplasmas isolated was carried out by biochemical and serological tests (disc growth inhibition and ... | 1979 | 455110 |
| identification of avian mycoplasma isolates by the agar-gel precipitin test. | recent isolates of mycoplasma gallisepticum and mycoplasma synoviae were readily typed by the agar-gel precipitin test with antigens prepared by freezing and thawing, sonic vibration, or sodium dodecyl sulfate. specific antisera prepared in rabbits or in foot-pad-inoculated chickens were adequate for culture typing. relatively few sera from chickens and turkeys in naturally infected flocks reacted positively. the precipitin reaction was highly specific, however. | 1977 | 410403 |
| culturing anomalies associated with mycoplasma recovered from the tissues of chicks and turkey poults experimentally infected with mycoplasma gallisepticum or mycoplasma gallinarum. | tissues of mycoplasma infected chicks and turkey poults were cultured and subcultured on mycoplasma agar. usually, colonies which grew on the agar initially inoculated could be subcultured, but sometimes they could not. at other times, colonies were not seen on the agar initially inoculated but appeared on the subcultured plate. | 1978 | 353927 |
| [studies on the specificity of the hemagglutination inhibition test using mycoplasma gallisepticum and mycoplasma synoviae in experimentally inoculated chickens]. | 1977 | 340190 | |
| control of mycoplasma gallisepticum in commercial laying chickens using artificial exposure to connecticut f strain mycoplasma gallisepticum. | 1979 | 298929 | |
| [the "vaccination reaction" syndrome of broilers after vaccination against newcastle disease and infectious bronchitis (author's transl)]. | in a part of the broiler flocks vaccinated against newcastle disease (n.c.d) and infectious bronchitis (i.b.), disease symptoms of lingering nature have been observed, generally in the second half of the rearing period. in a practical investigation with weekly examinations of chickens, supplemented by a serological examination of twenty-four animals per flock at the age of six weeks, it was hoped to establish the factors responsible for this "vaccination reaction". in the district under notice t ... | 1976 | 179162 |